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Pesquisa : Meningite [Descritor de assunto] OR Mening$ [Palavras]
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Id: 798444 LILACS-Express
Autor: Sander Andrade Portella Júnior, Caio; Rehem Machado, Marcelle; Chaves Lelis, Lucas; Fonseca Dias, Jefferson; Eduardo Ribeiro Wanderley Filho, Luiz; Antonio Guimarães Bastos, Carlos.
Título: Criptococoma cerebral e pulmonar em paciente imunocompetente: relato de caso / Cerebral and Pulmonary Cryptococcoma in an Immunocompetent Pacient: Case Report
Fonte: Arq. bras. neurocir;34(4):337-337, dez.2015.
Idioma: pt.
Resumo: Neurocriptococose é uma infecção fúngica do sistema nervoso central e sua principal apresentação clínica é a meningoencefalite. Lesões sólidas com aspecto tumoral, denominadas criptococoma, podem manifestar-se principalmente em imunodeprimidos. É relatado caso de paciente jovem, imunocompetente, com lesão tumoral frontal direita com grande efeito de massa e lesão menor em lobo occipital esquerdo, assim como lesão tumoral em pulmão direito. A paciente e seus cuidadores concordaram e autorizaram a publicação do caso. Foi feita revisão da literatura quanto à epidemiologia, tratamento e prognóstico. A paciente foi tratada comanfotericina B lipossomal e submetida à ressecção cirúrgica das lesões cerebrais e pulmonar, em tempos distintos, com bom desfecho clínico. A criptococose é infecção comum que pode acometer indivíduos tanto imunocomprometidos quanto imunocompetentes. Embora a meningoencefalite seja a forma mais comum de acometimento do SNC, existem outras formas mais raras como criptococoma cerebral. Há evidência de que a abordagem cirúrgica das lesões expansivas é benéfica e recomendada.

Neurocryptococcosis is a fungal infection of the central nervous system and its major clinical manifestation is meningoencephalitis. Solid tumor-like lesions, referred as cryptococcoma, usually occurs in immunocompromised patients. This paper reports a young immunocompetent female with a tumor-like lesion on the right frontal region and another one on the left occipital lobe, as well as a tumor lesion on her right lung. The patient and her caregivers have agreed and authorized the publishing of the case. Literature review wasmade regarding the epidemiology, treatment and prognosis. The patient was treated with anfotericine B and underwent surgical resection of the brain and lung lesions, at different times, with good clinical outcome. C. neoformans is a common fungal infection that can affect both immunocompetent and immunocompromised individuals. Although meningoencephalitis is themost common presentation of CNS involvement, there are other rarer forms such as cerebral cryptococcoma. There is evidence that the surgical treatment of mass lesions is beneficial and recommended.
Responsável: BR840


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Id: 798211 LILACS-Express
Autor: Davalos, Long; Terrazas, Yessica; Quintana, Ana; Egoavil, Martha; Sedano, Katherine; Castillo, María E.; Reyes, Isabel; Chaparro, Eduardo; Silva, Wilda; Campos, Francisco; Saenz, Andrés; Hernandez, Roger; Águila, Olguita del; Pinto, Daniel Guillén; Ochoa, Theresa J..
Título: Características epidemiológicas, clínicas y bacteriológicas de meningitis neumocócica en pacientes pediátricos de Lima, Perú / Epidemiologic, clinical and bacteriologic characteristics of pneumococcal meningitis in pediatric patients from Lima, Peru
Fonte: Rev. peru. med. exp. salud publica;33(3):425-431, jul.-sep. 2016. tab.
Idioma: es.
Resumo: RESUMEN Objetivos. Describir las características clínicas, letalidad, susceptibilidad antibiótica y distribución de serotipos de meningitis neumocócica en pacientes pediátricos de Lima, Perú. Materiales y Métodos. Serie de casos de meningitis neumocócica en niños menores de 16 años. Los datos fueron obtenidos de dos estudios multicéntricos prospectivos, de vigilancia pasiva de enfermedad neumocócica invasiva realizados en Lima-Perú desde los años 2006 al 2008, y del 2009 al 2011. Resultados. Reportamos 44 episodios de meningitis neumocócica; 68,2% fueron en niños menores de 2 años. La tasa de letalidad fue 32,6; y 92,9% de los casos letales ocurrieron en niños menores de dos años (p<0,05). La desnutrición estuvo asociada a los casos letales (p<0,05). El 64,3% de los casos fatales murieron dentro de los 2 primeros días. El 41,9% de los cultivos con neumococo fueron resistentes a la penicilina, 23,3% mostró resistencia intermedia a ceftriaxona (ninguno mostró resistencia completa) y 9,3% mostró resistencia a cloranfenicol. Los serotipos más frecuentes fueron 6B, 14, 19F y 23F, los cuales constituyeron el 68,3% de todas las cepas; 84,1% de las cepas encontradas están incluidas en los serotipos de la vacuna 13 valente. Conclusiones. La meningitis neumocócica continúa siendo una enfermedad letal, especialmente en niños menores de 2 años. Dado que aproximadamente dos tercios de los casos letales fallecen en las primeras 48 h, es crítico un diagnóstico y tratamiento oportuno, así como asegurar el cumplimiento de la inmunización con la vacuna neumocócica.(AU)

ABSTRACT Objectives. To describe the clinical characteristics, lethality, antibiotic susceptibility, and serotype distribution of pneumococcal meningitis in pediatric patients in Lima, Peru. Materials and Methods. A case series of pneumococcal meningitis in children less than 16 years of age from two prospective, multicenter, passive surveillance studies of invasive pneumococcal diseases held in Lima-Peru from 2006 to 2008 and 2009 to 2011. Results. We report 44 pneumococcal meningitis episodes; 68.2% of them were in children less than 2 years old. The overall case fatality rate was 32.6%; 92.9% of fatal cases were in children less than 2 years of age (p<0.05). Malnutrition was associated with fatal cases (p<0.05). 64.3% of fatal cases died within the first two days. 41.9% of pneumococcal isolates were resistant to penicillin, 23.3% were intermediate resistant to ceftriaxone (none were highly resistant) and 9.3% were resistant to chloramphenicol. The most common serotypes were 6B, 14, 19F and 23F, which accounted for 68.3% of all strains; 84.1% of strains were PCV13 serotypes. Conclusions. Pneumococcal meningitis continues to be a lethal disease, especially in children less than 2 years of age. Since almost two third of lethal cases lead to death within the first 48 hours, prompt diagnosis and management is critical, as well as assurance of immunization with pneumococcal vaccine.(AU)
Responsável: BR1.1 - BIREME


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Colombo, Silvia
Id: 798124 LILACS-Express
Autor: Prata, Juliana Anacleto Cabral; Souza, Celso Eduardo de; Angerami, Rodrigo Nogueira; Barbosa, Taíse Marongio Cotrim de Moraes; Santos, Fabiana Cristina Pereira dos; Colombo, Silvia; Guercio, Vânia Martins Fontes Del; Donalísio, Maria Rita.
Título: Antibodies for Rickettsia spp. in patients with negative serology for dengue virus, leptospirosis, and meningococcal disease in municipalities of São Paulo State, Brazil
Fonte: Rev. Soc. Bras. Med. Trop;49(5):567-571, Sept.-Oct. 2016. tab.
Idioma: en.
Resumo: Abstract INTRODUCTION Brazilian spotted fever is an infectious disease with a high mortality rate if not treated early. Differential diagnosis is difficult, as the first clinical signs are non-specific and can be confused with other diseases. The aim of the study was to investigate evidence of infection with Rickettsia rickettsii and Rickettsia parkeri in negative sera samples, collected in 2014, from patients with suspected leptospirosis, dengue fever, and meningococcal disease in Atibaia and Bragança Paulista municipalities of the State of São Paulo. METHODS The samples stored at the Institute Adolfo Lutz in Campinas were tested using an indirect immunofluorescence assay (IFA) with IgG and IgM against R. rickettsii and R. parkeri. Real-time polymerase chain reaction (PCR) testing was performed for the sera samples of patients who died (n = 3), those with initial suspicion of meningococcal disease (n = 6), and those with positive IFA results. RESULTS Of 258 samples from Bragança Paulista, 4 (1.6%) were positive, with IgG titers of 1:64 and 1:128 against R. rickettsii and R. parkeri, respectively. Of 155 samples from Atibaia, 2 (1.3%) were positive, with IgG titers of 1:64 and 1:128 against R. rickettsii and R. parkeri, respectively. No sample showed positive PCR results. CONCLUSIONS This serological investigation suggests there is evidence of exposure to Rickettsia spp. in residents of areas that have environmental conditions favorable to the spread of bacteria, in which Brazilian spotted fever incidence was not previously confirmed.(AU)
Responsável: BR1.1 - BIREME


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Id: 797742 LILACS-Express
Autor: Pérez Torga, Jesús Ernesto; Román Rubio, Pedro Antonio; García Sánchez, Iraimis; Castillo Arocha, Ivette; Bencomo Rodríguez, Llimia; Marcos Gutiérrez, Yamilé.
Título: Meningocele sacro anterior que simula quiste de ovario tabicado / Anterior sacral meningocele simulating ovarian cyst baffled
Fonte: Rev. cuba. obstet. ginecol;42(2):214-215, abr.-jun. 2016. ilus.
Idioma: es.
Resumo: El meningocele sacro anterior es una rara anomalía genética asociada a enfermedades del tejido conectivo como el síndrome de Marfán, síndrome de Loeys-Dietz, la neurofibromatosis tipo 1 y otras. El objetivo del presente trabajo es presentar el caso de un feto con meningocele sacro anterior, inicialmente diagnosticado como un gran quiste de ovario multilobulado, en una paciente con diagnóstico de síndrome de Marfan. Se trata de una herniación del saco dural, a través de agujeros sacros amplios y deformados por ectasia dural, ocupada mayormente por líquido cefalorraquídeo. La punción de estas estructuras o su rotura en el acto operatorio se ha asociado a complicaciones graves e incluso la muerte.(AU)

The anterior sacral meningocele is a rare genetic anomaly associated with connective tissue diseases such as Marfan syndrome, Loeys-Dietz syndrome, neurofibromatosis type 1 and others. The aim of this paper is to present the case of a fetus with anterior sacral meningocele, initially diagnosed as a large multilobulated ovarian cyst in a patient diagnosed with Marfan syndrome It is a herniation of the dural sac, through large sacral foramina, and deformed by dural ectasia, occupying mostly by cerebrospinal fluid. Puncture or rupture these structures during surgery has been associated with serious complications and even death.(AU)
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: 797598 LILACS-Express
Autor: Varona Rodríguez, Francisco; García Sánchez, José; Pérez Cruz, Beatriz; Avilés Álvarez, Adela.
Título: Diagnóstico etiológico presuntivo de las meningoencefalitis agudas en niños entre 1 y 14 años / Presumptive etiologic diagnosis of acute meningoencephalitis in children from 1 to 4 years old
Fonte: Arch. méd. Camaguey;6(6):623-631, nov.-dic. 2002.
Idioma: es.
Resumo: Se estudiaron 324 niños entre 1 y 14 años atendidos en 1999 en el servicio de urgencias del Hospital Pediátrico Provincial Eduardo Agramonte Piña, para determinar los elementos clínicos y de laboratorio sobre los que se basa el diagnóstico etiológico presuntivo de las meningoencefalitis agudas. Se encontró que la edad, el tiempo entre el inicio de los síntomas y la atención médica, así como el estado general, no permiten discriminar con precisión si se trata de una etiología viral o bacteriana. Los elementos de laboratorio descritos como típicos de las meningitis bacterianas aparecen en un escaso número de pacientes, en los cuales el predominio de leucocitos en sangre y líquido cefalorraquídeo fue el principal parámetro que se tuvo en cuenta para definir la supuesta causa del proceso. La tinción de Gram demostró presencia de gérmenes en sólo un 17, 9 %, y el cultivo del fluido espinal corroboró el diagnóstico inicial en un 66, 1 % de los pacientes, lo que evidenció un sobrediagnóstico de la causa bacteriana en nuestro medio.(AU)

324 children aged 1 to 14 years seen in 1999 in the service studied Provincial Emergency Eduardo Agramonte Piña Pediatric Hospital, to determine the clinical and laboratory elements on which based presumptive etiologic diagnosis of meningoencephalitis acute. It was found that age, the time between the onset of symptoms and health care, as well as the general state and not allow discrimination precisely if it is a viral or bacterial etiology. The laboratory elements described as typical of meningitis bacterial appear in a small number of patients, in which the predominance of leukocytes in blood and cerebrospinal fluid was main parameter to be taken into account to define the suspected cause of process. Gram stain showed the presence of germs in just a 17, 9%, and spinal fluid cultivation confirmed the initial diagnosis 66, 1% of patients, which showed an overdiagnosis of bacterial cause in our midst.(AU)
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: 797580 LILACS-Express
Autor: Rivero García, Cristina; Méndez, Magalys; Tamayo, Maria Elena.
Título: La tomografía axial computarizada en los procesos expansivos intraorbitarios / Axial Computed Tomography (ACT) for the intraorbitary expansive processess
Fonte: Arch. méd. Camaguey;6(5):492-500, sep.-oct. 2002.
Idioma: es.
Resumo: Se realizó un estudio analítico transversal de las TAC de órbitas realizadas en el período de diciembre de 1998 a diciembre del 2000 en el Hospital Provincial Manuel Ascunce Domenech de Camagüey con sospecha clara de procesos expansivos intraorbitarios, a los que se logró confirmación diagnóstica. El exoftalmos fue observado en la generalidad de los pacientes y la causa inflamatoria asociada a sinusitis fue la más frecuente, seguida por el aumento de volumen de estructuras musculares e incremento del tejido adiposo en la endocrinopatía tiroidea. Entre los procesos malignos se encontró el linfoma en el adulto, el glioma del nervio óptico y el reticoblastoma en el niño como entidades más frecuentes. Otros procesos observados fueron el carcinoma de senos maxilares, el meningioma, carcinoma de la glándula lagrimal, metástasis.(AU)

An analytical cross-sectional study of the axial computed tomography (ACT) of orbit carried out within the period from december 1998 to december 2000 at Manuel Ascunce Domenech provincial hospital of Camagüey, with clear assumption of expansive intraorbitary processes to which a diagnostic confirmation was achieved . Exophtalmus was observed in the majority of cases and the inflamatory cause associated to sinusitis was the frequent followed by the augmentation of the falty tissue in the thyroid endocrinopathy. Among malignant processes, adult lymphoma, optic nerve glyoma and retinoblastoma in the child were found as the most frequent entities. Other processes observed were maxillary sinuses carcinoma, melanoma metastasis in adults and the rabdoyosarcoma in children. As well as osteoma, inflamatory pseudomotor, dermoid tumor, hematomas and arteriovenous fistula in adults, and infant osseous displasias. The helicoid tomography computed axial with multipolar reconstructions contributed to the diagnosis and space location and to the extension of process in all cases.(AU)
Responsável: CU1.1 - Biblioteca Médica Nacional


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Id: 797449 LILACS-Express
Autor: Ropero Vera, Ana Rosa; Rodríguez, Jorge Martín; Farfán, Guillermo.
Título: Mortalidad por meningitis por Pasteurella canis: Oportunidades de aprendizaje / Mortality meningitis Pasteurella canis: Learning opportunities
Fonte: Salud UNINORTE;32(1):179-184, ene.-abr. 2016.
Idioma: es.
Resumo: La meningitis bacteriana es una enfermedad importante de distribución mundial, causa mayor y sustancial de mortalidad y morbilidad en países en desarrollo. La Organización Mundial de la Salud (OMS) sostiene que la meningitis es una de las diez afecciones principales del ser humano y debe ser considerada como una emergencia infectológica; por eso es fundamental reconocer que esta enfermedad es causa de muerte en niños de todo el mundo, sin distinción de raza, nivel económico o sociocultural. Se realizó una investigación de caso en menor de 53 días de nacido, que cumplía con los criterios clínicos y de laboratorio compatible con meningitis bacteriana, con el propósito de analizar y fortalecer la toma de decisiones en salud pública por parte de la secretaría local de salud del municipio de Valledupar (Colombia). Entre los hallazgos se encontró antecedentes infecciosos en el menor, coloración de Gram y cultivo de LCR, en el que se identificó cocobacilos Gram negativos, que fueron aislados como agente causal Pasteurella canis. Este estudio pretende sensibilizar a los prestadores de salud para que cuenten con personal altamente capacitado para brindar tratamientos adecuados y prevenir complicaciones en la meningitis bacteriana en niños, y así disminuir la posibilidad de secuelas o muerte, tanto en pacientes con compromiso inmunológico o sin este.(AU)

Bacterial meningitis is an important substantial worldwide disease, and a major cause of mortality and morbidity in developing countries. The World Health Organization (WHO) says that meningitis is one of the top ten diseases that affect humans and should be considered as an infectious diseases specialist emergency, it is essential to recognize that this disease causes death in children worldwide regardless of race, economic status or social and cultural. An investigation of the case in less than 53 days old, who met the clinical criteria and compatible laboratory with bacterial meningitis, in order to analyze and strengthen decision making in public health by the local health department came forward the municipality of Valledupar (Colombia). Among the findings infectious history was found in the lower, Gram stain and culture of CSF where Gram negative coccobacillary identified, isolated as a causative agent Pasteurella canis. This study aims to sensitize health care providers to have highly trained staff to provide adequate treatment and prevent complications of bacterial meningitis in children and lessen the possibility of sequelae or death, both in patients with and without immune compromise.(AU)
Responsável: CO332 - Facultad de Medicina


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Id: 797297 LILACS-Express
Autor: Real D., Raúl Emilio; Valenzuela F., Alejandro Jesús.
Título: HIPONATREMIA EN PACIENTES ADULTOS CON LESIÓN CEREBRAL AGUDAS / HYPONATREMIA IN ADULT PATIENTS WITH ACUTE BRAIN INJURY
Fonte: Rev. cientif. cienc. med;19(1):22-28, 2016. ilus.
Idioma: es.
Resumo: Las afecciones agudas del sistema nervioso central como los accidentes cerebrovasculares (ACV) y traumatismos de cráneo producen alteraciones en la homeostasis del sodio por diversos mecanismos. La hiponatremia es un fenómeno frecuente en la práctica clínica, constituye un desafío debido a la diversa etiología, las complicaciones de su evolución natural llegan a ser muy severas y hasta fatales. Objetivo: determinar la frecuencia y causas de hiponatremia en pacientes adultos con daño cerebral agudo internados en el Hospital Nacional. Materiales y metodos: estudio descriptivo, retrospectivo de corte transversal que incluyó 115 pacientes internados en el Hospital Nacional (Itauguá, Paraguay) en 2015. Se consideró como hiponatremia en cerebro agudo a los portadores de accidente cerebral vascular (ACV) hemorrágico (intra parenquimatoso, subaracnoideo o cerebro meníngeo), ACV isquémico o traumatismo de cráneo corroborados por tomografía axial computada de cráneo o resonancia magnética de menos de 10 días de evolución y hayan presentado Na sérico ≤ 135 mEq/L. Resultados: la prevalencia de hiponatremia fue 34%, con predominio del sexo masculino (54%). El diagnóstico más frecuente fue el ACV hemorrágico y la principal comorbilidad asociada fue la hipertensión arterial. Se diagnosticaron 15 pacientes con Síndrome de secreción inadecuada de hormona antidiurética y 6 con depleción cerebral de sal que representan 38,4% y 15,4% respectivamente. Conclusión: se halló alta prevalencia de hiponatremia en pacientes con daño cerebral agudo. El Síndrome de secreción inadecuada de hormona antidiurética y depleción cerebral de sal describen y explican con mayor frecuencia la presencia de hiponatremia asociada a natriuresis aumentada.(AU)

The acute afections of center sistem nervous as the stroke(ACV) and head trauma produce alterations in the Sodio Homeostasis For various mechanisms.The Hiponatremia is a frecuently event in our enviroment, It is a Desafy because to diverse etiology, the complications of their natural evolution become severity and to fatalytis. Objective: To determine the frequency and causes of hyponatremia in adult patients with acute brain injury admitted to the National Hospital. Materials and methods: descriptive, cross-sectional prospective study that included 1 15 patients in the National Hospital (Itauguá, Paraguay) in 2015. It was considered as hyponatremia in acute brain carriers of hemorrhagic stroke (intracerebral, subarachnoid), ischemic stroke or head injury confirmed by computed tomography or magnetic resonance image in patients submitted less than 10 days and serum Na + ≤ 135 mEq/L. Results: The prevalence of hyponatremia was 34%, with a predominance of males (54%). The most common diagnosis was hemorrhagic stroke and the most common comorbidity was arterial hypertension. There were diagnosed 15 patients with Syndrome of inappropriate ADH secretion and 6 with Cerebral salt wasting syndrome representing 38.4% and 15.4% respectively. Conclusion: we found high prevalence of hyponatremia in patients with acute brain damage. The Syndrome of inappropriate ADH secretion and Cerebral salt wasting syndrome are described as the 2 syndromes that more frequently explain the presence of hyponatremia associated with increased natriuresis.(AU)
Responsável: BO132.1 - Dirección General de Servicios de Salud


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Id: 797104
Autor: Nunes, Patricia Renck; Oliveira, Marianne Schrader.
Título: Prevalência de Streptococcus agalactiae em gestantes da Grande Porto Alegre, RS: relato de caso / Prevalence of Streptococcus agalactiae in pregnant women of Porto Alegre, RS: case report
Fonte: Rev. bras. anal. clin;47(4):178-180, 2015. graf.
Idioma: pt.
Resumo: O Streptococcus agalactiae é um coco Gram-positivo, beta-hemolítico que faz parte da microbiota de membranas mucosas, colonizando, principalmente, o trato intestinal e o genitourinário. A identificação no trato anogenital das gestantes é de importância para asaúde da mulher e do feto, visto que esse microrganismo pode causar infecções neonatais graves, septicemia, pneumonia e meningite neonatal, assim como causar infecção no organismo materno e comprometer a evolução da gestação. Cento e quarenta e quatrogestantes foram submetidas à coleta de secreção vaginal e perianal para cultura em meio Stuart. A amostra da secreção vaginal foi inoculada em agar sangue e a amostra da secreção anal em meio azida, as quais foram incubadas a 37°C, por 24 horas, seguindo-se o teste de CAMP. Foi possível verificar a alta frequência de colonização por S.agalactiae, neste estudo (40%), principalmente em pacientes entre a 34ª e a 37ª semanas de gestação (64%), o que ressalta a importância da inclusão da cultura de secreção vaginal e perianal para pesquisa desse microrganismo nos exames pré-natais...(AU)
Descritores: Streptococcus agalactiae
Prevalência
Gravidez
Membranas Extraembrionárias
Mortalidade Infantil
Limites: Humanos
Feminino
Gravidez
Adolescente
Adulto Jovem
Adulto
Meia-Idade
Tipo de Publ: Relatos de Casos
Responsável: BR408.1 - Biblioteca Setorial


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Almeida, Sérgio Monteiro de
Id: 796838 LILACS-Express
Autor: Almeida, Sérgio Monteiro de; Raboni, Sônia Mara; Nogueira, Meri Bordignon; Vidal, Luine R. Renaud.
Título: Red blood cells in cerebrospinal fluid as possible inhibitory factor for enterovirus RT-PCR / Presença de hemácias no líquido cefalorraquidiano como possível fator inibitório da RT-PCR para detecção de enterovirus
Fonte: Arq. neuropsiquiatr;74(10):810-815, Oct. 2016. tab, graf.
Idioma: en.
Resumo: ABSTRACT The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics of viral meningitis were assessed for EV by RT-PCR. Viral RNA extracted with guanidine isothyocianate buffer and virus detection was performed by in-house nested PCR. Positivity for EV RT-PCR was higher in CSF samples without RBCs than in samples with RBCs: 13(26%) and 36(9.2%), p = 0.001. In the group with positive EV RT-PCR, the mean + SD CSF RBC was 37 ± 183 cell/mm3; the group with negative results had 580 + 2,890 cell/mm3 (p = 0.007). The acceptable upper limit for CSF RBCs that could not influence RT-PCR was 108 cells/mm3. CSF samples with negative results for EV RT-PCR have more erythrocytes.(AU)

RESUMO A presença de hemoglobina em amostras de fluidos corporais é considerada um fator inibitório importante da reação em cadeia da polimerase (PCR). O objetivo deste estudo era examinar a influencia de hemácias no líquido cefalorraquidiano (LCR) como um fator inibitório da RT-PCR para enterovirus (EV). Quatrocentos e quarenta amostras de LCR de pacientes com características de meningite viral foram avaliados para enterovirus por RT-PCR. RNA viral foi extraído com tampão de isotiocianato de guanidina e a detecção viral foi feita com nested PCR in-house. A positividade do EV RT-PCR no LCR foi maior nas amostras de LCR sem hemácias do que as amostras com hemácias: 13 (26%) e 36 (9,2%), respectivamente (p = 0,001). No grupo com resultados EV RT-PCR positivo, a media ± DP do número de hemácias no LCR foi 37 ± 183 cell/mm3 e no grupo com resultados negativos foi 580 ± 2.890 cell/mm3 (p = 0,007). O limite superior aceitável de hemácias no LCR para não inibir o resultado do PCR foi 108 cells/mm3. As amostras de LCR com resultados negativos para RT-PCR EV tem mais eritrócitos em comparação com amostras com resultados positivos.(AU)
Responsável: BR1.1 - BIREME



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