Base de datos : MEDLINE
Búsqueda : Bacterias [Palabras]
Referencias encontradas : 958648 [refinar]
Mostrando: 1 .. 10   en el formato [Detallado]

página 1 de 95865 va a la página                         

  1 / 958648 MEDLINE  
              next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29073176
[Au] Autor:Vatter T; Maurer A; Kopahnke D; Perovic D; Ordon F; Pillen K
[Ad] Dirección:Institute for Resistance Research and Stress Tolerance, Julius Kuehn-Institute, Federal Research Centre for Cultivated Plants, Quedlinburg, Germany.
[Ti] Título:A nested association mapping population identifies multiple small effect QTL conferring resistance against net blotch (Pyrenophora teres f. teres) in wild barley.
[So] Fuente:PLoS One;12(10):e0186803, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:The net form of net blotch caused by the necrotrophic fungus Pyrenophora teres f. teres is a major disease of barley, causing high yield losses and reduced malting and feed quality. Exploiting the allelic richness of wild barley proved to be a valuable tool to broaden the genetic base of resistance of modern elite cultivars. In this study, a SNP-based nested association mapping (NAM) study was conducted to map QTL for P. teres resistance in the barley population HEB-25 comprising 1,420 lines derived from BC1S3 generation. By scoring the percentage of infected leaf area followed by calculation of the average ordinate (AO) and scoring of the reaction type (RT) in two-year field trials a large variability of net blotch resistance across and within families of HEB-25 was observed. Genotype response to net blotch infection showed a range of 48.2% for AO (0.9-49.1%) and 6.4 for RT (2.2-8.6). NAM based on 5,715 informative SNPs resulted in the identification of 24 QTL for resistance against net blotch. Out of these, six QTL are considered novel showing no correspondence to previously reported QTL for net blotch resistance. Overall, variation of net blotch resistance in HEB-25 turned out to be controlled by small effect QTL. Results indicate the presence of alleles in HEB-25 differing in their effect on net blotch resistance. Results provide valuable information regarding the genetic architecture of the complex barley-P. teres f. teres interaction as well as for the improvement of net blotch resistance of elite barley cultivars.
[Mh] Términos MeSH primario: Ascomicetos
Resistencia a la Enfermedad/genética
Genotipo
Hordeum/genética
Enfermedades de las Plantas/genética
Polimorfismo de Nucleótido Simple
Sitios de Carácter Cuantitativo
[Mh] Términos MeSH secundario: Hordeum/microbiología
Enfermedades de las Plantas/microbiología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171026
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186803


  2 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29073145
[Au] Autor:Fitzpatrick C; Asiedu K; Sands A; Gonzalez Pena T; Marks M; Mitja O; Meheus F; Van der Stuyft P
[Ad] Dirección:Department of Control of Neglected Tropical Diseases, World Health Organization, Geneva, Switzerland.
[Ti] Título:The cost and cost-effectiveness of rapid testing strategies for yaws diagnosis and surveillance.
[So] Fuente:PLoS Negl Trop Dis;11(10):e0005985, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:BACKGROUND: Yaws is a non-venereal treponemal infection caused by Treponema pallidum subspecies pertenue. The disease is targeted by WHO for eradication by 2020. Rapid diagnostic tests (RDTs) are envisaged for confirmation of clinical cases during treatment campaigns and for certification of the interruption of transmission. Yaws testing requires both treponemal (trep) and non-treponemal (non-trep) assays for diagnosis of current infection. We evaluate a sequential testing strategy (using a treponemal RDT before a trep/non-trep RDT) in terms of cost and cost-effectiveness, relative to a single-assay combined testing strategy (using the trep/non-trep RDT alone), for two use cases: individual diagnosis and community surveillance. METHODS: We use cohort decision analysis to examine the diagnostic and cost outcomes. We estimate cost and cost-effectiveness of the alternative testing strategies at different levels of prevalence of past/current infection and current infection under each use case. We take the perspective of the global yaws eradication programme. We calculate the total number of correct diagnoses for each strategy over a range of plausible prevalences. We employ probabilistic sensitivity analysis (PSA) to account for uncertainty and report 95% intervals. RESULTS: At current prices of the treponemal and trep/non-trep RDTs, the sequential strategy is cost-saving for individual diagnosis at prevalence of past/current infection less than 85% (81-90); it is cost-saving for surveillance at less than 100%. The threshold price of the trep/non-trep RDT (below which the sequential strategy would no longer be cost-saving) is US$ 1.08 (1.02-1.14) for individual diagnosis at high prevalence of past/current infection (51%) and US$ 0.54 (0.52-0.56) for community surveillance at low prevalence (15%). DISCUSSION: We find that the sequential strategy is cost-saving for both diagnosis and surveillance in most relevant settings. In the absence of evidence assessing relative performance (sensitivity and specificity), cost-effectiveness is uncertain. However, the conditions under which the combined test only strategy might be more cost-effective than the sequential strategy are limited. A cheaper trep/non-trep RDT is needed, costing no more than US$ 0.50-1.00, depending on the use case. Our results will help enhance the cost-effectiveness of yaws programmes in the 13 countries known to be currently endemic. It will also inform efforts in the much larger group of 71 countries with a history of yaws, many of which will have to undertake surveillance to confirm the interruption of transmission.
[Mh] Términos MeSH primario: Técnicas y Procedimientos Diagnósticos/economía
Buba/diagnóstico
Buba/economía
[Mh] Términos MeSH secundario: Estudios de Cohortes
Análisis Costo-Beneficio
Técnicas y Procedimientos Diagnósticos/instrumentación
Técnicas y Procedimientos Diagnósticos/estadística & datos numéricos
Erradicación de la Enfermedad/economía
Monitoreo Epidemiológico
Humanos
Prevalencia
Sensibilidad y Especificidad
Treponema pallidum/aislamiento & purificación
Buba/epidemiología
Buba/microbiología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171026
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005985


  3 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29069031
[Au] Autor:Zhu J; Jiang Y; Shi Y; Zheng B; Xu Z; Jia W
[Ad] Dirección:aDepartment of Ophthalmology, The Affiliated Guangren Hospital of Xi'an Jiaotong University College of Medicine bDepartment of Otorhinolaryngology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
[Ti] Título:Clinical manifestations and treatment outcomes of syphilitic uveitis in HIV-negative patients in China: A retrospective case study.
[So] Fuente:Medicine (Baltimore);96(43):e8376, 2017 Oct.
[Is] ISSN:1536-5964
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Syphilitic chorioretinitis should be included in differential diagnosis of any form of ocular inflammation. A significantly higher proportion of human immunodeficiency virus (HIV)-positive patients with ocular syphilis as compared to HIV-negative cases have been reported in published studies. However, the clinical signs and symptoms are more insidious in HIV-negative patients who are easily misdiagnosed. We report a series of cases of ocular syphilis and describe the clinical manifestations and treatment outcomes of syphilitic chorioretinitis in HIV-negative patients in China.This was a retrospective case series study. The clinical records of patients with syphilis chorioretinitis were reviewed. Demographic information and findings of fundus fluorescein angiography (FFA), indocyanine green angiography (ICGA), and spectral domain optical coherence tomography (SD-OCT) were analyzed. All patients received the standard treatment. Ophthalmology examination and laboratory evaluation were repeated every 3 months. All changes were recorded. The treatment was considered successful if the patients had no inflammation in both eyes and rapid plasma reagin titer was negative after therapy.The study examined 41 eyes of 28 HIV-negative patients. The main complaints were blurry vision, floaters, and visual field defect. Twenty-seven eyes presented with panuveitis, and all had posterior involvement, including uveitis, vasculitis, chorioretinitis, and optic neuritis. The most common manifestations were uveitis and retinal vasculitis. Disc hyperfluorescence and persistent dark spots were the most common findings on FFA and ICGA. The ill-defined inner segment/outer segment junction was the most frequent manifestation on SD-OCT. Patients were diagnosed with syphilitic uveitis based on positive serological tests. Best-corrected visual acuity (BCVA) was improved in 34 eyes after treatment. Eleven patients were misdiagnosed before serological tests were performed. The delay in treatment led to long-standing cystoid macular edema and optic neuropathy, which were associated with poor BCVA (P = .037).The common manifestations of syphilitic chorioretinitis were uveitis, retinal vasculitis, and optic neuritis. Further diagnosis should be prompted by FFA, ICGA, and SD-OCT when ocular manifestation is suspected. The standard treatment for neurosyphilis was effective. If patients are presumed to be in low-risk groups such as HIV-negative, delays in diagnosis, and therapy may be likely. It is necessary to reiterate the importance of including syphilis uveitis as a differential diagnosis for any form of ocular inflammations, especially posterior uveitis and optic neuropathy.
[Mh] Términos MeSH primario: Coriorretinitis/microbiología
Infecciones Bacterianas del Ojo/complicaciones
Sífilis/complicaciones
Uveítis/microbiología
[Mh] Términos MeSH secundario: Adulto
Anciano
Antibacterianos/uso terapéutico
China
Coriorretinitis/quimioterapia
Infecciones Bacterianas del Ojo/quimioterapia
Infecciones Bacterianas del Ojo/microbiología
Femenino
Angiografía con Fluoresceína
Humanos
Masculino
Mediana Edad
Neuritis Óptica/quimioterapia
Neuritis Óptica/microbiología
Vasculitis Retiniana/quimioterapia
Vasculitis Retiniana/microbiología
Estudios Retrospectivos
Sífilis/quimioterapia
Sífilis/microbiología
Tomografía de Coherencia Óptica
Resultado del Tratamiento
Uveítis/quimioterapia
Agudeza Visual
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Anti-Bacterial Agents)
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:171025
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008376


  4 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29069028
[Au] Autor:Ruiz-Mesa JD; Marquez-Gomez I; Sena G; Buonaiuto VA; Ordoñez JM; Salido M; Ciézar AP; Santis LV; Mediavilla C; Colmenero JD
[Ad] Dirección:aInfectious Diseases Department bMicrobiology Department cCritical Care and Emergency Departments, Regional University Hospital dInstituto de Investigación Biomedica de Málaga (IBIMA), Malaga, Spain.
[Ti] Título:Factors associated with severe sepsis or septic shock in complicated pyelonephritis.
[So] Fuente:Medicine (Baltimore);96(43):e8371, 2017 Oct.
[Is] ISSN:1536-5964
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Severe sepsis or septic shock are the main factors influencing the prognosis of acute pyelonephritis (APN). Our aim was to analyze factors associated with the development of severe sepsis or septic shock in a large sample of patients with acute complicated pyelonephritis (ACPN).This prospective observational study comprised 1507 consecutive patients aged 14 years or older who were admitted to a tertiary care hospital because of ACPN between 1997 and 2015. Covariates associated in univariate analysis with severe sepsis or septic shock were then analyzed by multivariate logistic regression.Of the 1507 patients, 423 (28.1%) fulfilled the criteria for severe sepsis or septic shock at the time of admission. Crude and attributable mortality at 30 days were 17.7% and 11.7% in patients with severe sepsis or septic shock versus 1.7% and 0.6% in patients without severe sepsis or septic shock, P < .0001 and P < .0005, respectively. An age > 65 years, urinary instrumentation in the previous 2 weeks, the lack of mictional syndrome or costovertebral tenderness, an ectasia ≥ grade II, and bacteremia were independent risk factors associated with severe sepsis or septic shock.The prevalence of severe sepsis and septic shock in patients with ACPN is high. Some factors associated with severe sepsis are easy to identify in any emergency department. The information provided here could be useful when deciding which patients should be admitted to receive immediate treatment.
[Mh] Términos MeSH primario: Pielonefritis/microbiología
Sepsis/mortalidad
Choque Séptico/mortalidad
[Mh] Términos MeSH secundario: Enfermedad Aguda
Anciano
Femenino
Hospitalización
Humanos
Modelos Logísticos
Masculino
Mediana Edad
Análisis Multivariante
Prevalencia
Estudios Prospectivos
Pielonefritis/mortalidad
Factores de Riesgo
Sepsis/microbiología
Choque Séptico/microbiología
[Pt] Tipo de publicación:JOURNAL ARTICLE; OBSERVATIONAL STUDY
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:171025
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008371


  5 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29069025
[Au] Autor:Lai SW; Lin CL; Liao KF
[Ad] Dirección:aCollege of Medicine, China Medical University, Taichung bDepartment of Family Medicine, China Medical University Hospital, Taichung cManagement Office for Health Data, China Medical University Hospital, Taichung dCollege of Medicine, Tzu Chi University, Hualien eDepartment of Internal Medicine, Taichung Tzu Chi General Hospital, Taichung, Taiwan.
[Ti] Título:Head and neck cancer associated with increased rate of pulmonary tuberculosis in a population-based cohort study.
[So] Fuente:Medicine (Baltimore);96(43):e8366, 2017 Oct.
[Is] ISSN:1536-5964
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:The objective of this study was to examine the incidence and hazard ratio (HR) of pulmonary tuberculosis in patients with head and neck cancer in Taiwan.This population-based retrospective cohort study was conducted to analyze the database of the Taiwan National Health Insurance Program. There were 2522 subjects aged 20 to 84 years with newly diagnosed head and neck cancer as the head and neck cancer group between 2000 and 2012, and 10,064 randomly selected sex- and age-matched subjects without any cancer as the noncancer group. The incidence of pulmonary tuberculosis at the end of 2013 was estimated in both groups. A multivariable Cox proportional hazards regression model was used to estimate the HR and 95% confidence interval (CI) for pulmonary tuberculosis being associated with head and neck cancer.The overall incidence of pulmonary tuberculosis was 2.86-fold greater in the head and neck cancer group than that in the noncancer group (4.70 vs 1.64 per 1000 person-years, 95% CI, 2.53-3.24). After adjusting for confounding factors, the adjusted HR of pulmonary tuberculosis became 2.90 for the head and neck cancer group (95% CI, 2.11-3.99), compared with the noncancer group. In addition, male (adjusted HR 2.27, 95% CI, 1.29-4.00) and age (increase for 1 year, adjusted HR 1.06, 95% CI, 1.05-1.08) were associated with pulmonary tuberculosis.Head and neck cancer is significantly associated with 2.90-fold increased hazard of pulmonary tuberculosis in Taiwan, compared with the general population.
[Mh] Términos MeSH primario: Neoplasias de Cabeza y Cuello/microbiología
Tuberculosis Pulmonar/epidemiología
[Mh] Términos MeSH secundario: Adulto
Factores de Edad
Bases de Datos Factuales
Femenino
Humanos
Incidencia
Masculino
Mediana Edad
Modelos de Riesgos Proporcionales
Estudios Retrospectivos
Factores de Riesgo
Factores Sexuales
Taiwán/epidemiología
Tuberculosis Pulmonar/etiología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:171025
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000008366


  6 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29054229
[Au] Autor:Kayawake H; Chen-Yoshikawa TF; Oda H; Motoyama H; Hamaji M; Menju T; Aoyama A; Sato T; Sonobe M; Date H
[Ad] Dirección:Department of Thoracic Surgery, Kyoto University, Kyoto, Japan.
[Ti] Título:Complications of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration.
[So] Fuente:Ann Thorac Surg;104(5):e363-e365, 2017 Nov.
[Is] ISSN:1552-6259
[Cp] País de publicación:Netherlands
[La] Idioma:eng
[Ab] Resumen:Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is considered useful for the staging and diagnosis of lung cancer or thoracic lymph node enlargement; however, little is known about its complications. Between July 2009 and November 2016, 413 patients underwent EBUS-TBNA, and four complications (0.97%) occurred. Here we report four cases involving complications of EBUS-TBNA, including mediastinitis (n = 2), obstructive pneumonia (n = 1), and airway obstruction requiring admission to the intensive care unit (n = 1). All patients recovered with appropriate medical treatment. Despite their low incidence, the complications associated with EBUS-TBNA can be serious.
[Mh] Términos MeSH primario: Biopsia por Aspiración con Aguja Fina Guiada por Ultrasonido Endoscópico/efectos adversos
Neoplasias Pulmonares/patología
Ganglios Linfáticos/patología
Mediastinitis/etiología
[Mh] Términos MeSH secundario: Anciano
Obstrucción de las Vías Aéreas/diagnóstico por imagen
Obstrucción de las Vías Aéreas/etiología
Obstrucción de las Vías Aéreas/terapia
Estudios de Cohortes
Biopsia por Aspiración con Aguja Fina Guiada por Ultrasonido Endoscópico/métodos
Endosonografía/efectos adversos
Endosonografía/métodos
Femenino
Humanos
Neoplasias Pulmonares/cirugía
Ganglios Linfáticos/cirugía
Masculino
Mediastinitis/diagnóstico por imagen
Mediastinitis/terapia
Mediana Edad
Invasividad Neoplásica/patología
Estadificación de Neoplasias
Neumonía Bacteriana/etiología
Neumonía Bacteriana/microbiología
Neumonía Bacteriana/terapia
Pronóstico
Radiografía Torácica/métodos
Estudios Retrospectivos
Medición de Riesgo
Tomografía Computarizada por Rayos X/métodos
Resultado del Tratamiento
[Pt] Tipo de publicación:CASE REPORTS; JOURNAL ARTICLE
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:171021
[St] Status:MEDLINE


  7 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29049365
[Au] Autor:Kader M; Alaoui-El-Azher M; Vorhauer J; Kode BB; Wells JZ; Stolz D; Michalopoulos G; Wells A; Scott M; Ismail N
[Ad] Dirección:Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America.
[Ti] Título:MyD88-dependent inflammasome activation and autophagy inhibition contributes to Ehrlichia-induced liver injury and toxic shock.
[So] Fuente:PLoS Pathog;13(10):e1006644, 2017 Oct.
[Is] ISSN:1553-7374
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Severe hepatic inflammation is a common cause of acute liver injury following systemic infection with Ehrlichia, obligate Gram-negative intracellular bacteria that lack lipopolysaccharide (LPS). We have previously shown that type I IFN (IFN-I) and inflammasome activation are key host-pathogenic mediators that promote excessive inflammation and liver damage following fatal Ehrlichia infection. However, the underlying signals and mechanisms that regulate protective immunity and immunopathology during Ehrlichia infection are not well understood. To address this issue, we compared susceptibility to lethal Ixodes ovatus Ehrlichia (IOE) infection between wild type (WT) and MyD88-deficient (MyD88-/-) mice. We show here that MyD88-/- mice exhibited decreased inflammasome activation, attenuated liver injury, and were more resistant to lethal infection than WT mice, despite suppressed protective immunity and increased bacterial burden in the liver. MyD88-dependent inflammasome activation was also dependent on activation of the metabolic checkpoint kinase mammalian target of rapamycin complex 1 (mTORC1), inhibition of autophagic flux, and defective mitophagy in macrophages. Blocking mTORC1 signaling in infected WT mice and primary macrophages enhanced bacterial replication and attenuated inflammasome activation, suggesting autophagy promotes bacterial replication while inhibiting inflammasome activation. Finally, our data suggest TLR9 and IFN-I are upstream signaling mechanisms triggering MyD88-mediated mTORC1 and inflammasome activation in macrophages following Ehrlichia infection. This study reveals that Ehrlichia-induced liver injury and toxic shock are mediated by MyD88-dependent inflammasome activation and autophagy inhibition.
[Mh] Términos MeSH primario: Ehrlichiosis/inmunología
Inflamasomas/metabolismo
Fallo Hepático Agudo/microbiología
Factor 88 de Diferenciación Mieloide/metabolismo
Choque Séptico/metabolismo
[Mh] Términos MeSH secundario: Animales
Autofagia/inmunología
Western Blotting
Modelos Animales de Enfermedad
Ehrlichia/inmunología
Ehrlichiosis/metabolismo
Ensayo de Inmunoadsorción Enzimática
Femenino
Citometría de Flujo
Técnica del Anticuerpo Fluorescente
Procesamiento de Imagen Asistida por Computador
Etiquetado Corte-Fin in Situ
Inflamasomas/inmunología
Fallo Hepático Agudo/inmunología
Fallo Hepático Agudo/metabolismo
Ratones
Ratones Consanguíneos C57BL
Ratones Noqueados
Microscopía Confocal
Microscopía Electrónica de Transmisión
Factor 88 de Diferenciación Mieloide/inmunología
Reacción en Cadena en Tiempo Real de la Polimerasa
Choque Séptico/inmunología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Inflammasomes); 0 (Myd88 protein, mouse); 0 (Myeloid Differentiation Factor 88)
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171019
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006644


  8 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29040128
[Au] Autor:Hegde V; Dworsky EM; Stavrakis AI; Loftin AH; Zoller SD; Park HY; Richman S; Johansen D; Hu Y; Taylor JA; Hamad CD; Chun RF; Xi W; Adams JS; Bernthal NM
[Ad] Dirección:1Department of Orthopaedic Surgery, David Geffen School of Medicine at the University of California, Los Angeles, Los Angeles, California.
[Ti] Título:Single-Dose, Preoperative Vitamin-D Supplementation Decreases Infection in a Mouse Model of Periprosthetic Joint Infection.
[So] Fuente:J Bone Joint Surg Am;99(20):1737-1744, 2017 Oct 18.
[Is] ISSN:1535-1386
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:BACKGROUND: Despite recent advances, infection remains the most common etiology of arthroplasty failure. Recent work suggests that 25-hydroxyvitamin D (25D) deficiency correlates with the frequency of periprosthetic joint infection (PJI). We endeavored to examine whether 25D3 deficiency leads to increased bacterial burden in vivo in an established mouse model of PJI and, if so, whether this effect can be reversed by preoperative 25D3 supplementation. METHODS: Mice (lys-EGFP) possessing fluorescent neutrophils were fed a vitamin D3-sufficient (n = 20) or deficient (n = 40) diet for 6 weeks. A group of 25D3-deficient mice (n = 20) were "rescued" with 1 intraperitoneal dose of 25D3 at 3 days before surgery. A stainless steel implant was inserted into the knee joint and the joint space was inoculated with bioluminescent Staphylococcus aureus (1 × 10 colony forming units [CFUs]). In vivo imaging was used to monitor bacterial burden and neutrophil infiltration. Blood was drawn to confirm 25D3 levels 3 days before surgery and on postoperative days (PODs) 0 and 14. Mice were killed at POD 21, and CFUs were quantified after culture. Myeloperoxidase (MPO) and ß-N-acetylglucosaminidase (NAG) were assayed to look at neutrophil infiltration and activated tissue macrophage recruitment, respectively. RESULTS: Serum values confirmed 25D3 deficiency and repletion of the 25D3-rescued group. Bacterial bioluminescence and neutrophil fluorescence were significantly greater (p < 0.05) in the 25D3-deficient group. CFU counts from the joint tissue and implant were also significantly greater in this group (p < 0.05). Rescue treatment significantly decreased bacterial burden and neutrophil infiltration (p < 0.05). Compared with the 25D3-sufficient and 25D3-rescued groups, MPO activity was higher (p < 0.02) and NAG activity was lower (p < 0.03) in the 25D3-deficient group. CONCLUSIONS: This study demonstrated in vivo in a mouse model of PJI that (1) 25D3 deficiency results in increased bacterial burden and neutrophil infiltration, and (2) this effect can be reversed with preoperative repletion of 25D3. CLINICAL RELEVANCE: Considering that >65% of patients undergoing arthroplasty have insufficient or low levels of total 25D and that 25D levels can be replenished with ease using a U.S. Food and Drug Administration (FDA)-approved, oral 25D3 product, 25D deficiency may be an important modifiable risk factor in humans undergoing joint replacement.
[Mh] Términos MeSH primario: Suplementos Dietéticos
Prótesis de la Rodilla/efectos adversos
Infecciones Relacionadas con Prótesis/prevención & control
Infecciones Estafilocócicas/prevención & control
Deficiencia de Vitamina D/quimioterapia
Vitamina D/análogos & derivados
Vitaminas/uso terapéutico
[Mh] Términos MeSH secundario: Animales
Artroplastia de Reemplazo de Rodilla
Carga Bacteriana
Biomarcadores/sangre
Esquema de Medicación
Inyecciones Intraperitoneales
Masculino
Ratones
Infiltración Neutrófila
Cuidados Preoperatorios/métodos
Infecciones Relacionadas con Prótesis/etiología
Infecciones Relacionadas con Prótesis/microbiología
Distribución Aleatoria
Factores de Riesgo
Infecciones Estafilocócicas/etiología
Infecciones Estafilocócicas/microbiología
Vitamina D/sangre
Vitamina D/uso terapéutico
Deficiencia de Vitamina D/complicaciones
Deficiencia de Vitamina D/diagnóstico
Deficiencia de Vitamina D/microbiología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Biomarkers); 0 (Vitamins); 1406-16-2 (Vitamin D); 64719-49-9 (25-hydroxyvitamin D)
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:171017
[St] Status:MEDLINE
[do] DOI:10.2106/JBJS.16.01598


  9 / 958648 MEDLINE  
              first record previous record next record last record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29038320
[Au] Autor:Sadarangani M; Sell T; Iro MA; Snape MD; Voysey M; Finn A; Heath PT; Bona G; Esposito S; Diez-Domingo J; Prymula R; Odueyungbo A; Toneatto D; Pollard AJ; European MenB Vaccine Study Group
[Ad] Dirección:Oxford Vaccine Group, Department of Paediatrics (Sadarangani, Sell, Iro, Snape, Voysey, Pollard), University of Oxford, and the NIHR Oxford Biomedical Research Centre, Oxford, UK; Vaccine Evaluation Center (Sadarangani), BC Children's Hospital Research Institute, The University of British Columbia,
[Ti] Título:Persistence of immunity after vaccination with a capsular group B meningococcal vaccine in 3 different toddler schedules.
[So] Fuente:CMAJ;189(41):E1276-E1285, 2017 Oct 16.
[Is] ISSN:1488-2329
[Cp] País de publicación:Canada
[La] Idioma:eng
[Ab] Resumen:BACKGROUND: One schedule for the capsular group B meningococcal vaccine 4CMenB is 2 doses that are administered 2 months apart for children aged 12-23 months, with a booster dose 12-24 months later. Our objective was to provide data on persistence of human serum bactericidal antibody (hSBA) titres in children up to 4 years of age after initial doses at 12-24 months, and immunogenicity of a booster dose at 48 months of age compared with vaccine-naive children. METHODS: Children previously immunized, as part of a randomized controlled trial, with 2 doses of 4CMenB vaccine at 12-24 months of age received a booster at 4 years of age. Vaccine-naive age-matched toddlers received 2 doses of 4CMenB. Human serum bactericidal antibody titres against reference strains H44/76, 5/99, NZ98/254 and M10713 were evaluated before and after innoculation with 4CMenB vaccine in 4-year-old children. RESULTS: Of 332 children in the study, 123 had previously received 4CMenB and 209 were vaccine-naive controls. Before the booster, the proportions of participants (previously vaccinated groups compared with controls) with hSBA titres of 1:5 or more were as follows: 9%-11% v. 1% (H44/76), 84%-100% v. 4% (5/99), 0%-18% v. 0% (NZ98/254) and 59%-60% v. 60% (M10713). After 1 dose of 4CMenB in previously immunized children, the proportions of participants achieving hSBA titres of 1:5 or more were 100% (H44/76 and 5/99), 70%-100% (NZ98/254) and 90%-100% (M10713). INTERPRETATION: We found that waning of hSBA titres by 4 years of age occurred after 2 doses of 4CMenB vaccine administered at 12-24 months, and doses at 12-24 months have a priming effect on the immune system. A booster may be necessary to maintain hSBA titres of 1:5 or more among those children with increased disease risk. : ClinicalTrials.gov, no. NCT01717638.
[Mh] Términos MeSH primario: Meningitis Meningocócica/prevención & control
Vacunas Meningococicas/administración & dosificación
Neisseria meningitidis Serogrupo B/inmunología
Vacunación/métodos
[Mh] Términos MeSH secundario: Anticuerpos Antibacterianos/sangre
Preescolar
Relación Dosis-Respuesta a Droga
Femenino
Estudios de Seguimiento
Humanos
Lactante
Masculino
Meningitis Meningocócica/microbiología
Estudios Retrospectivos
Resultado del Tratamiento
[Pt] Tipo de publicación:JOURNAL ARTICLE; MULTICENTER STUDY; RANDOMIZED CONTROLLED TRIAL
[Nm] Nombre de substancia:
0 (4CMenB vaccine); 0 (Antibodies, Bacterial); 0 (Meningococcal Vaccines)
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:171017
[St] Status:MEDLINE
[do] DOI:10.1503/cmaj.161288


  10 / 958648 MEDLINE  
              first record previous record
selecciona
para imprimir
Fotocopia
Texto completo
[PMID]:29028799
[Au] Autor:Kracalik IT; Kenu E; Ayamdooh EN; Allegye-Cudjoe E; Polkuu PN; Frimpong JA; Nyarko KM; Bower WA; Traxler R; Blackburn JK
[Ad] Dirección:Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, FL, United States of America.
[Ti] Título:Modeling the environmental suitability of anthrax in Ghana and estimating populations at risk: Implications for vaccination and control.
[So] Fuente:PLoS Negl Trop Dis;11(10):e0005885, 2017 Oct.
[Is] ISSN:1935-2735
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Anthrax is hyper-endemic in West Africa. Despite the effectiveness of livestock vaccines in controlling anthrax, underreporting, logistics, and limited resources makes implementing vaccination campaigns difficult. To better understand the geographic limits of anthrax, elucidate environmental factors related to its occurrence, and identify human and livestock populations at risk, we developed predictive models of the environmental suitability of anthrax in Ghana. We obtained data on the location and date of livestock anthrax from veterinary and outbreak response records in Ghana during 2005-2016, as well as livestock vaccination registers and population estimates of characteristically high-risk groups. To predict the environmental suitability of anthrax, we used an ensemble of random forest (RF) models built using a combination of climatic and environmental factors. From 2005 through the first six months of 2016, there were 67 anthrax outbreaks (851 cases) in livestock; outbreaks showed a seasonal peak during February through April and primarily involved cattle. There was a median of 19,709 vaccine doses [range: 0-175 thousand] administered annually. Results from the RF model suggest a marked ecological divide separating the broad areas of environmental suitability in northern Ghana from the southern part of the country. Increasing alkaline soil pH was associated with a higher probability of anthrax occurrence. We estimated 2.2 (95% CI: 2.0, 2.5) million livestock and 805 (95% CI: 519, 890) thousand low income rural livestock keepers were located in anthrax risk areas. Based on our estimates, the current anthrax vaccination efforts in Ghana cover a fraction of the livestock potentially at risk, thus control efforts should be focused on improving vaccine coverage among high risk groups.
[Mh] Términos MeSH primario: Vacunas contra el Carbunco
Carbunco/epidemiología
Carbunco/veterinaria
Brotes de Enfermedades/veterinaria
Ganado
[Mh] Términos MeSH secundario: Algoritmos
Animales
Carbunco/microbiología
Carbunco/prevención & control
Bacillus anthracis/aislamiento & purificación
Bovinos
Enfermedades de los Bovinos/microbiología
Enfermedades de los Bovinos/prevención & control
Clima
Simulación por Computador
Ambiente
Métodos Epidemiológicos
Ghana/epidemiología
Humanos
Concentración de Iones de Hidrógeno
Ganado/microbiología
Factores de Riesgo
Suelo/química
Vacunación
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Anthrax Vaccines); 0 (Soil)
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:171102
[Lr] Fecha última revisión:171102
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171013
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0005885



página 1 de 95865 va a la página                         
   


Refinar la búsqueda
  Base de datos : MEDLINE Formulario avanzado   

    Buscar en el campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPS/OMS - Centro Latinoamericano y del Caribe de Información en Ciencias de la Salud