|
|
| [PMID]: | 22876746 |
| [Au] Autor: | van Beek N; Rentzsch K; Probst C; Komorowski L; Kasperkiewicz M; Fechner K; Bloecker IM; Zillikens D; Stöcker W; Schmidt E |
| [Ad] Dirección: | Department of Dermatology, University of Luebeck, Luebeck, Germany. |
| [Ti] Título: | Serological diagnosis of autoimmune bullous skin diseases: prospective comparison of the BIOCHIP mosaic-based indirect immunofluorescence technique with the conventional multi-step single test strategy. |
| [So] Fuente: | Orphanet J Rare Dis;7:49, 2012. | | [Is] ISSN: | 1750-1172 |
| [Cp] País de publicación: | England |
| [La] Idioma: | eng |
| [Ab] Resumen: | BACKGROUND: Various antigen-specific immunoassays are available for the serological diagnosis of autoimmune bullous diseases. However, a spectrum of different tissue-based and monovalent antigen-specific assays is required to establish the diagnosis. BIOCHIP mosaics consisting of different antigen substrates allow polyvalent immunofluorescence (IF) tests and provide antibody profiles in a single incubation. METHODS: Slides for indirect IF were prepared, containing BIOCHIPS with the following test substrates in each reaction field: monkey esophagus, primate salt-split skin, antigen dots of tetrameric BP180-NC16A as well as desmoglein 1-, desmoglein 3-, and BP230gC-expressing human HEK293 cells. This BIOCHIP mosaic was probed using a large panel of sera from patients with pemphigus vulgaris (PV, n=65), pemphigus foliaceus (PF, n=50), bullous pemphigoid (BP, n=42), and non-inflammatory skin diseases (n=97) as well as from healthy blood donors (n=100). Furthermore, to evaluate the usability in routine diagnostics, 454 consecutive sera from patients with suspected immunobullous disorders were prospectively analyzed in parallel using a) the IF BIOCHIP mosaic and b) a panel of single antibody assays as commonly used by specialized centers. RESULTS: Using the BIOCHIP mosaic, sensitivities of the desmoglein 1-, desmoglein 3-, and NC16A-specific substrates were 90%, 98.5% and 100%, respectively. BP230 was recognized by 54% of the BP sera. Specificities ranged from 98.2% to 100% for all substrates. In the prospective study, a high agreement was found between the results obtained by the BIOCHIP mosaic and the single test panel for the diagnosis of BP, PV, PF, and sera without serum autoantibodies (Cohen's κ between 0.88 and 0.97). CONCLUSIONS: The BIOCHIP mosaic contains sensitive and specific substrates for the indirect IF diagnosis of BP, PF, and PV. Its diagnostic accuracy is comparable with the conventional multi-step approach. The highly standardized and practical BIOCHIP mosaic will facilitate the serological diagnosis of autoimmune blistering diseases. |
| [Mh] Términos MeSH primario: |
Enfermedades Autoinmunes/diagnóstico
Técnica del Anticuerpo Fluorescente Indirecta/métodos
Enfermedades Cutáneas Vesiculoampollosas/diagnóstico
|
| [Mh] Términos MeSH secundario: |
Secuencia de Bases
Cartilla de ADN
Células HEK293
Humanos
Estudios Prospectivos
|
| [Pt] Tipo de publicación: | COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; VALIDATION STUDIES |
[Nm] Nombre de substancia:
| 0 (DNA Primers) |
| [Em] Mes de ingreso: | 1304 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Fecha de ingreso para procesamiento: | 130101 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.1186/1750-1172-7-49 |
|
