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  1 / 2315 MEDLINE  
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[PMID]:29272280
[Au] Autor:Yin H; Zhao L; Jiang X; Li S; Huo H; Chen H
[Ad] Dirección:State Key Laboratory of Veterinary Biotechnology, Heilongjiang Provincial Key Laboratory of Laboratory Animal and Comparative Medicine, Harbin Veterinary Research Institute, the Chinese Academy of Agriculture Science, Harbin, P. R. China.
[Ti] Título:DEV induce autophagy via the endoplasmic reticulum stress related unfolded protein response.
[So] Fuente:PLoS One;12(12):e0189704, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Duck enteritis virus (DEV) can infect ducks, geese, and many other poultry species and leads to acute, septic and highly fatal infectious disease. Autophagy is an evolutionarily ancient pathway that plays an important role in many viral infections. We previously reported that DEV infection induces autophagy for its own benefit, but how this occurs remains unclear. In this study, endoplasmic reticulum (ER) stress was triggered by DEV infection, as demonstrated by the increased expression of the ER stress marker glucose-regulated protein 78 (GRP78) and the dilated morphology of the ER. Pathways associated with the unfolded protein response (UPR), including the PKR-like ER protein kinase (PERK) and inositol-requiring enzyme 1 (IRE1) pathways, but not the activating transcription factor 6 (ATF6) pathway, were activated in DEV-infected duck embryo fibroblast (DEF) cells. In addition, the knockdown of both PERK and IRE1 by small interfering RNAs (siRNAs) reduced the level of LC3-II and viral yields, which suggested that the PERK-eukaryotic initiation factor 2α (eIF2α) and IRE1-x-box protein1 (XBP1) pathways may contribute to DEV-induced autophagy. Collectively, these data offer new insight into the mechanisms of DEV -induced autophagy through activation of the ER stress-related UPR pathway.
[Mh] Términos MeSH primario: Autofagia/fisiología
Estrés del Retículo Endoplásmico
Mardivirus/fisiología
Respuesta de Proteína Desplegada
[Mh] Términos MeSH secundario: Animales
Western Blotting
Células Cultivadas
Patos
Electroforesis en Gel de Poliacrilamida
Microscopía Electrónica de Transmisión
Interferencia de ARN
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mes de ingreso:1801
[Cu] Fecha actualización por clase:180116
[Lr] Fecha última revisión:180116
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171223
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189704


  2 / 2315 MEDLINE  
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[PMID]:29267390
[Au] Autor:Gurung A; Kamble N; Kaufer BB; Pathan A; Behboudi S
[Ad] Dirección:The Pirbright Institute, Ash Road, Woking, United Kingdom.
[Ti] Título:Association of Marek's Disease induced immunosuppression with activation of a novel regulatory T cells in chickens.
[So] Fuente:PLoS Pathog;13(12):e1006745, 2017 12.
[Is] ISSN:1553-7374
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Marek's Disease Virus (MDV) is an alphaherpesvirus that infects chickens, transforms CD4+ T cells and causes deadly lymphomas. In addition, MDV induces immunosuppression early during infection by inducing cell death of the infected lymphocytes, and potentially due to activation of regulatory T (Treg)-cells. Furthermore, immunosuppression also occurs during the transformation phase of the disease; however, it is still unknown how the disease can suppress immune response prior or after lymphoma formation. Here, we demonstrated that chicken TGF-beta+ Treg cells are found in different lymphoid tissues, with the highest levels found in the gut-associated lymphoid tissue (cecal tonsil: CT), fostering an immune-privileged microenvironment exerted by TGF-beta. Surprisingly, significantly higher frequencies of TGF-beta+ Treg cells are found in the spleens of MDV-susceptible chicken lines compared to the resistant line, suggesting an association between TGF-beta+ Treg cells and host susceptibility to lymphoma formation. Experimental infection with a virulent MDV elevated the levels of TGF-beta+ Treg cells in the lungs as early as 4 days post infection, and during the transformation phase of the disease in the spleens. In contrast to TGF-beta+ Treg cells, the levels of CD4+CD25+ T cells remained unchanged during the infection and transformation phase of the disease. Furthermore, our results demonstrate that the induction of TGF-beta+ Treg cells is associated with pathogenesis of the disease, as the vaccine strain of MDV did not induce TGF-beta+ Treg cells. Similar to human haematopoietic malignant cells, MDV-induced lymphoma cells expressed high levels of TGF-beta but very low levels of TGF-beta receptor I and II genes. The results confirm that COX-2/ PGE2 pathway is involved in immunosuppression induced by MDV-lymphoma cells. Taken together, our results revealed a novel TGF-beta+ Treg subset in chickens that is activated during MDV infection and tumour formation.
[Mh] Términos MeSH primario: Tolerancia Inmunológica/inmunología
Activación de Linfocitos/inmunología
Enfermedad de Marek/inmunología
Subgrupos de Linfocitos T/inmunología
Linfocitos T Reguladores/inmunología
[Mh] Términos MeSH secundario: Animales
Transformación Celular Viral/fisiología
Pollos
Factor de Crecimiento Transformador beta/inmunología
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nombre de substancia:
0 (Transforming Growth Factor beta)
[Em] Mes de ingreso:1801
[Cu] Fecha actualización por clase:180111
[Lr] Fecha última revisión:180111
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171222
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006745


  3 / 2315 MEDLINE  
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[PMID]:28730521
[Au] Autor:Yang C; Zhang B; Li J; Huang X; Liu D; Hou L; Li Q; Li H
[Ad] Dirección:China Institute of Veterinary Drug Control, No. 8 Zhongguancun South Street, Haidian District, Beijing, 100081, People's Republic of China. ychenghuai@163.com.
[Ti] Título:Complete genomic sequence of a duck enteritis virus attenuated via serial passage in chick embryos.
[So] Fuente:Arch Virol;162(11):3549-3550, 2017 Nov.
[Is] ISSN:1432-8798
[Cp] País de publicación:Austria
[La] Idioma:eng
[Ab] Resumen:Here, we present the complete genomic sequence of an attenuated duck enteritis virus (DEV). The Chinese standard challenge strain of DEV (DEV CSC) was serially passaged 20 times in chick embryo fibroblasts and then 85 times in chick embryos. The virus was attenuated and was avirulent to 2-month-old ducks. The attenuated DEV genome is 162,131 base pairs (bp) in length and as long as the parental genomic sequence. There are only 22 nucleotide substitutions, resulting in single amino acid changes in open reading frames LORF5, LORF4, UL41, UL39, UL32, UL13, UL10, UL3, US3, US4 and US7. The genome sequence has been deposited in the GenBank database under accession number KU216226. This study provides genetic information about DEV attenuation and further advances our understanding of the molecular basis of DEV pathogenesis.
[Mh] Términos MeSH primario: Patos/virología
Genoma Viral
Mardivirus/fisiología
[Mh] Términos MeSH secundario: Animales
Células Cultivadas
Embrión de Pollo
ADN Viral/genética
Enteritis/veterinaria
Enteritis/virología
Fibroblastos/fisiología
Mardivirus/patogenicidad
Enfermedades de las Aves de Corral/virología
Virulencia
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (DNA, Viral)
[Em] Mes de ingreso:1710
[Cu] Fecha actualización por clase:171025
[Lr] Fecha última revisión:171025
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170722
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3491-1


  4 / 2315 MEDLINE  
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[PMID]:28678686
[Au] Autor:Chen S; Zhang W; Zhou Q; Wang A; Sun L; Wang M; Jia R; Zhu D; Liu M; Sun K; Yang Q; Wu Y; Chen X; Cheng A
[Ad] Dirección:3​Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, PR China 1​Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China 2​Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agr
[Ti] Título:Cross-species antiviral activity of goose interferon lambda against duck plague virus is related to its positive self-regulatory feedback loop.
[So] Fuente:J Gen Virol;98(6):1455-1466, 2017 Jun.
[Is] ISSN:1465-2099
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:Duck plague virus (DPV) is a virus of the Herpesviridae family that leads to acute disease with a high mortality rate in ducks. Control of the disease contributes to the development of poultry breeding. Type III IFN family (IFN-λs) is a novel member of the IFN family, and goose IFN-λ (goIFN-λ) is a newly identified gene whose antiviral function has only been investigated to a limited extent. Here, the cross-species antiviral activity of goIFN-λ against DPV in duck embryo fibroblasts (DEFs) was studied. We found that pre-treatment with goIFN-λ greatly increased the expression of IFN-λ in both heterologous DEFs and homologous goose embryo fibroblasts (GEFs), while differentially inducing IFNα- and IFN-stimulated genes. Additionally, a positive self-regulatory feedback loop of goIFN-λ was blocked by a mouse anti-goIFN-λ polyclonal antibody, which was confirmed in both homologous GEFs and goose peripheral blood mononuclear cells (PBMCs). The suppression of the BAC-DPV-EGFP by goIFN-λ in DEFs was confirmed by fluorescence microscopy, flow cytometry (FCM) analysis, viral copies and titre detection, which can be rescued by mouse anti-goIFN-λ polyclonal antibody incubation. Finally, reporter gene assays indicated that the cross-species antiviral activity of goIFN-λ against BAC-DPV-EGFP is related to its positive self-regulatory feedback loop and subsequent ISG induction. Our data shed light on the fundamental mechanisms of goIFN-λ antiviral function in vitro and extend the considerable range of therapeutic applications in multiple-poultry disease.
[Mh] Términos MeSH primario: Antivirales/metabolismo
Interleucinas/metabolismo
Mardivirus/inmunología
[Mh] Términos MeSH secundario: Animales
Células Cultivadas
Patos
Fibroblastos/virología
Gansos
Leucocitos Mononucleares/virología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Antiviral Agents); 0 (Interleukins)
[Em] Mes de ingreso:1707
[Cu] Fecha actualización por clase:170717
[Lr] Fecha última revisión:170717
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170706
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000788


  5 / 2315 MEDLINE  
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[PMID]:28665725
[Au] Autor:Kennedy DA; Cairns C; Jones MJ; Bell AS; Salathé RM; Baigent SJ; Nair VK; Dunn PA; Read AF
[Ad] Dirección:A Center for Infectious Disease Dynamics, Departments of Biology and Entomology, The Pennsylvania State University, University Park, PA 16802.
[Ti] Título:Industry-Wide Surveillance of Marek's Disease Virus on Commercial Poultry Farms.
[So] Fuente:Avian Dis;61(2):153-164, 2017 Jun.
[Is] ISSN:1938-4351
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Marek's disease virus is a herpesvirus of chickens that costs the worldwide poultry industry more than US$1 billion annually. Two generations of Marek's disease vaccines have shown reduced efficacy over the last half century due to evolution of the virus. Understanding where the virus is present may give insight into whether continued reductions in efficacy are likely. We conducted a 3-yr surveillance study to assess the prevalence of Marek's disease virus on commercial poultry farms, determine the effect of various factors on virus prevalence, and document virus dynamics in broiler chicken houses over short (weeks) and long (years) timescales. We extracted DNA from dust samples collected from commercial chicken and egg production facilities in Pennsylvania, USA. Quantitative PCR was used to assess wild-type virus detectability and concentration. Using data from 1018 dust samples with Bayesian generalized linear mixed effects models, we determined the factors that correlated with virus prevalence across farms. Maximum likelihood and autocorrelation function estimation on 3727 additional dust samples were used to document and characterize virus concentrations within houses over time. Overall, wild-type virus was detectable at least once on 36 of 104 farms at rates that varied substantially between farms. Virus was detected in one of three broiler-breeder operations (companies), four of five broiler operations, and three of five egg layer operations. Marek's disease virus detectability differed by production type, bird age, day of the year, operation (company), farm, house, flock, and sample. Operation (company) was the most important factor, accounting for between 12% and 63.4% of the variation in virus detectability. Within individual houses, virus concentration often dropped below detectable levels and reemerged later. These data characterize Marek's disease virus dynamics, which are potentially important to the evolution of the virus.
[Mh] Términos MeSH primario: Herpesvirus Gallináceo 2/aislamiento & purificación
Enfermedad de Marek/virología
Enfermedades de las Aves de Corral/virología
Vigilancia de Guardia/veterinaria
[Mh] Términos MeSH secundario: Crianza de Animales Domésticos/economía
Animales
Pollos
Granjas
Genotipo
Herpesvirus Gallináceo 2/clasificación
Herpesvirus Gallináceo 2/genética
Enfermedad de Marek/economía
Enfermedad de Marek/epidemiología
Pennsylvania
Enfermedades de las Aves de Corral/economía
Enfermedades de las Aves de Corral/epidemiología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1708
[Cu] Fecha actualización por clase:170823
[Lr] Fecha última revisión:170823
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170701
[St] Status:MEDLINE
[do] DOI:10.1637/11525-110216-Reg.1


  6 / 2315 MEDLINE  
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[PMID]:28591220
[Au] Autor:Dong K; Chang S; Xie Q; Black-Pyrkosz A; Zhang H
[Ad] Dirección:USDA, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan, United States of America.
[Ti] Título:Comparative transcriptomics of genetically divergent lines of chickens in response to Marek's disease virus challenge at cytolytic phase.
[So] Fuente:PLoS One;12(6):e0178923, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Marek's disease (MD), caused by Marek's disease virus (MDV), remains an economically significant threat to the poultry industry worldwide. Genetic resistance to MD is a promising alternative strategy to augment current control measures (vaccination and management). However, only a few functional genes reportedly conferring MD resistance have been identified. Here, we performed a comparative transcriptomics analysis of two highly inbred yet genetically divergent lines of chickens (line 63 and 72) that are resistant and susceptible to MD, respectively, in response to a very virulent plus strain of MDV (vv+MDV) challenge at cytolytic phase. A total of 203 DEGs in response to MDV challenge were identified in the two lines. Of these, 96 DEGs were in common for both lines, in addition to 36 and 71 DEGs that were specific for line 63 and 72, respectively. Functional enrichment analysis results showed the DEGs were significantly enriched in GO terms and pathways associated with immune response. Especially, the four DEGs, FGA, ALB, FN1, and F13A1 that reportedly facilitate virus invasion or immunosuppression, were found to be significantly up-regulated in the susceptible line 72 but down-regulated in the resistant line 63 birds. These results provide new resources for future studies to further elucidate the genetic mechanism conferring MD resistance.
[Mh] Términos MeSH primario: Pollos/genética
Pollos/virología
Perfilación de la Expresión Génica
Variación Genética
Herpesvirus Gallináceo 2/fisiología
Enfermedad de Marek/genética
Enfermedad de Marek/virología
[Mh] Términos MeSH secundario: Animales
Reacción en Cadena en Tiempo Real de la Polimerasa
Reproducibilidad de los Resultados
Análisis de Secuencia de ARN
[Pt] Tipo de publicación:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mes de ingreso:1709
[Cu] Fecha actualización por clase:170918
[Lr] Fecha última revisión:170918
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170608
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178923


  7 / 2315 MEDLINE  
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[PMID]:28548038
[Au] Autor:Chakraborty P; Vervelde L; Dalziel RG; Wasson PS; Nair V; Dutia BM; Kaiser P
[Ad] Dirección:1​The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK †â€‹Present address: Chittagong Veterinary and Animal Sciences University, Khulshi, Chittagong 4225, Bangladesh.
[Ti] Título:Marek's disease virus infection of phagocytes: a de novo in vitro infection model.
[So] Fuente:J Gen Virol;98(5):1080-1088, 2017 May.
[Is] ISSN:1465-2099
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:Marek's disease virus (MDV) is an alphaherpesvirus that induces T-cell lymphomas in chickens. Natural infections in vivo are caused by the inhalation of infected poultry house dust and it is presumed that MDV infection is initiated in the macrophages from where the infection is passed to B cells and activated T cells. Virus can be detected in B and T cells and macrophages in vivo, and both B and T cells can be infected in vitro. However, attempts to infect macrophages in vitro have not been successful. The aim of this study was to develop a model for infecting phagocytes [macrophages and dendritic cells (DCs)] with MDV in vitro and to characterize the infected cells. Chicken bone marrow cells were cultured with chicken CSF-1 or chicken IL-4 and chicken CSF-2 for 4 days to produce macrophages and DCs, respectively, and then co-cultured with FACS-sorted chicken embryo fibroblasts (CEFs) infected with recombinant MDV expressing EGFP. Infected phagocytes were identified and sorted by FACS using EGFP expression and phagocyte-specific mAbs. Detection of MDV-specific transcripts of ICP4 (immediate early), pp38 (early), gB (late) and Meq by RT-PCR provided evidence for MDV replication in the infected phagocytes. Time-lapse confocal microscopy was also used to demonstrate MDV spread in these cells. Subsequent co-culture of infected macrophages with CEFs suggests that productive virus infection may occur in these cell types. This is the first report of in vitro infection of phagocytic cells by MDV.
[Mh] Términos MeSH primario: Herpesvirus Gallináceo 2/fisiología
Fagocitos/virología
Replicación Viral
[Mh] Términos MeSH secundario: Animales
Células Cultivadas
Pollos
Técnicas de Cocultivo
Enfermedad de Marek/virología
Modelos Biológicos
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1707
[Cu] Fecha actualización por clase:171115
[Lr] Fecha última revisión:171115
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170527
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000763


  8 / 2315 MEDLINE  
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[PMID]:28510513
[Au] Autor:Teng M; Yu ZH; Zhao P; Zhuang GQ; Wu ZX; Dang L; Li HZ; Ma SM; Cui ZZ; Zhang GP; Wu R; Luo J
[Ad] Dirección:1​College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, PR China 2​Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450002, PR China.
[Ti] Título:Putative roles as oncogene or tumour suppressor of the Mid-clustered microRNAs in Gallid alphaherpesvirus 2 (GaHV2) induced Marek's disease lymphomagenesis.
[So] Fuente:J Gen Virol;98(5):1097-1112, 2017 May.
[Is] ISSN:1465-2099
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:In the last decade, numerous microRNAs (miRNAs) have been identified in diverse virus families, particularly in herpesviruses. Gallid alphaherpesvirus 2 (GaHV2) is a representative oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in its natural hosts, namely Marek's disease (MD). In the GaHV2 genome there are 26 mature miRNAs derived from 14 precursors assembled into three clusters, namely the Meq-cluster, Mid-cluster and LAT-cluster. Several GaHV2 miRNAs, especially those in the Meq-cluster (e.g. miR-M4-5p), have been demonstrated to be critical in MD pathogenesis and/or tumorigenesis. Interestingly the downstream Mid-cluster is regulated and transcribed by the same promoter as the Meq-cluster in the latent phase of the infection, but the role of these Mid-clustered miRNAs in GaHV2 biology remains unclear. We have generated the deletion mutants of the Mid-cluster and of its associated individual miRNAs in GX0101 virus, a very virulent GaHV2 strain, and demonstrated that the Mid-clustered miRNAs are not essential for virus replication. Using GaHV2-infected chickens as an animal model, we found that, compared with parental GX0101 virus, the individual deletion of miR-M31 decreased the mortality and gross tumour incidence of infected chickens while the deletion individually of miR-M1 or miR-M11 unexpectedly increased viral pathogenicity or oncogenicity, similarly to the deletion of the entire Mid-cluster region. More importantly, our data further confirm that miR-M11-5p, the miR-M11-derived mature miRNA, targets the viral oncogene meq and suppresses its expression in GaHV2 infection. We report here that members of the Mid-clustered miRNAs, miR-M31-3p and miR-M11-5p, potentially act either as oncogene or tumour suppressor in MD lymphomagenesis.
[Mh] Términos MeSH primario: Carcinógenos
Genes Supresores de Tumor
Interacciones Huésped-Patógeno
Linfoma de Células T
Mardivirus/fisiología
Enfermedad de Marek/complicaciones
MicroARNs/metabolismo
[Mh] Términos MeSH secundario: Experimentación Animal
Animales
Carcinogénesis
Eliminación de Gen
Mardivirus/genética
Enfermedad de Marek/patología
MicroARNs/genética
Análisis de Supervivencia
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Carcinogens); 0 (MicroRNAs)
[Em] Mes de ingreso:1707
[Cu] Fecha actualización por clase:171115
[Lr] Fecha última revisión:171115
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170517
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000786


  9 / 2315 MEDLINE  
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[PMID]:28475033
[Au] Autor:Mwangi WN; Vasoya D; Kgosana LB; Watson M; Nair V
[Ad] Dirección:1​Avian Viral Diseases Programme, UK-China Centre of Excellence on Avian Disease Research, The Pirbright Institute, Pirbright, Surrey, UK.
[Ti] Título:Differentially expressed genes during spontaneous lytic switch of Marek's disease virus in lymphoblastoid cell lines determined by global gene expression profiling.
[So] Fuente:J Gen Virol;98(4):779-790, 2017 Apr.
[Is] ISSN:1465-2099
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:Marek's disease virus (MDV), an alphaherpesvirus of poultry, causes Marek's disease and is characterized by visceral CD4+TCRαß+ T-cell lymphomas in susceptible hosts. Immortal cell lines harbouring the viral genome have been generated from ex vivo cultures of MD tumours. As readily available sources of large numbers of cells, MDV-transformed lymphoblastoid cell lines (LCLs) are extremely valuable for studies of virus-host interaction. While the viral genome in most cells is held in a latent state, minor populations of cells display spontaneous reactivation identifiable by the expression of lytic viral genes. Spontaneous reactivation in these cells presents an opportunity to investigate the biological processes involved in the virus reactivation. For detailed characterization of the molecular events associated with reactivation, we used two lymphoblastoid cell lines derived from lymphomas induced by pRB1B-UL47eGFP, a recombinant MDV engineered to express enhanced green fluorescent protein (EGFP) fused with the UL47. We used fluorescence-activated cell sorting to purify the low-frequency EGFP-positive cells with a spontaneously activating viral genome from the majority EGFP-negative cells and analysed their gene expression profiles by RNA-seq using Illumina HiSeq2500. Ingenuity pathway analysis on more than 2000 differentially expressed genes between the lytically infected (EGFP-positive) and latently infected (EGFP-negative) cell populations identified the biological pathways involved in the reactivation. Virus-reactivating cells exhibited differential expression of a significant number of viral genes, with hierarchical differences in expression levels. Downregulation of a number of host genes including those directly involved in T-cell activation, such as CD3, CD28, ICOS and phospholipase C, was also noticed in the LCL undergoing lytic switch.
[Mh] Términos MeSH primario: Perfilación de la Expresión Génica
Herpesvirus Gallináceo 2/genética
Enfermedad de Marek/virología
Enfermedades de las Aves de Corral/virología
Proteínas Virales/genética
[Mh] Términos MeSH secundario: Animales
Línea Celular Tumoral
Pollos
Regulación Viral de la Expresión Génica
Herpesvirus Gallináceo 2/fisiología
Linfoma/virología
Proteínas Virales/metabolismo
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Viral Proteins)
[Em] Mes de ingreso:1706
[Cu] Fecha actualización por clase:171115
[Lr] Fecha última revisión:171115
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170506
[St] Status:MEDLINE
[do] DOI:10.1099/jgv.0.000744


  10 / 2315 MEDLINE  
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[PMID]:28413051
[Au] Autor:Li K; Liu Y; Zhang Y; Gao L; Liu C; Cui H; Qi X; Gao Y; Zhong L; Wang X
[Ad] Dirección:Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China.
[Ti] Título:Protective efficacy of a novel recombinant Marek's disease virus vector vaccine against infectious bursal disease in chickens with or without maternal antibodies.
[So] Fuente:Vet Immunol Immunopathol;186:55-59, 2017 Apr.
[Is] ISSN:1873-2534
[Cp] País de publicación:Netherlands
[La] Idioma:eng
[Ab] Resumen:Infectious bursal disease (IBD) causes significant clinical and economic losses to the poultry industry worldwide. Current vaccine programs using live attenuated and inactivated vaccines have numerous drawbacks. As an alternative solution to control IBD, a Marek's disease virus (MDV) vector vaccine (rMDV-VP2) expressing the VP2 gene of infectious bursal disease virus (IBDV) has been developed. In this study, the protective efficacy of rMDV-VP2 was evaluated in a dose-related experiment which showed that a single dose of 1000 PFU was sufficient to fully protect chickens against IBDV infection. Chickens inoculated with lower doses of rMDV-VP2 (250 or 500 PFU) conferred 80 and 90% protection against IBDV. Next, rMDV-VP2 vaccine provided 90% protection against IBDV in commercial layer chickens with maternal antibodies, which was higher than the protective efficacy using the B87 live vaccine of IBDV. Additionally, rMDV-VP2 conferred effective protection against very virulent MDV challenge in chickens (95% for chickens vaccinated with 250 or 500 PFU and 100% for chickens vaccinated with 1000 or 2000 PFU). These results demonstrated that rMDV-VP2 may be a novel bivalent vaccine against IBD and Marek's disease in chickens.
[Mh] Términos MeSH primario: Anticuerpos Antivirales/inmunología
Infecciones por Birnaviridae/veterinaria
Pollos
Virus de la Enfermedad Infecciosa de la Bolsa
Vacunas contra la Enfermedad de Marek/administración & dosificación
Enfermedades de las Aves de Corral/prevención & control
Proteínas Estructurales Virales/inmunología
[Mh] Términos MeSH secundario: Animales
Infecciones por Birnaviridae/inmunología
Infecciones por Birnaviridae/prevención & control
Inmunidad Materno-Adquirida
Virus de la Enfermedad Infecciosa de la Bolsa/genética
Vacunas contra la Enfermedad de Marek/genética
Vacunas contra la Enfermedad de Marek/inmunología
Enfermedades de las Aves de Corral/inmunología
Vacunas Sintéticas/genética
Vacunas Sintéticas/inmunología
Proteínas Estructurales Virales/genética
[Pt] Tipo de publicación:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nombre de substancia:
0 (Antibodies, Viral); 0 (Marek Disease Vaccines); 0 (VP2 protein, infectious bursal disease virus); 0 (Vaccines, Synthetic); 0 (Viral Structural Proteins)
[Em] Mes de ingreso:1708
[Cu] Fecha actualización por clase:170821
[Lr] Fecha última revisión:170821
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170418
[St] Status:MEDLINE



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