| [PMID]: | 22687618 |
| [Au] Autor: | Weigand LA; Ford AP; Undem BJ |
| [Ad] Dirección: | Johns Hopkins University School of Medicine, The Johns Hopkins Asthma and Allergy Center, 5501 Hopkins Bayview Circle 1A62, Baltimore, MD21224, USA. |
| [Ti] Título: | A role for ATP in bronchoconstriction-induced activation of guinea pig vagal intrapulmonary C-fibres. |
| [So] Fuente: | J Physiol;590(Pt 16):4109-20, 2012 Aug 15. |
| [Is] ISSN: | 1469-7793 |
| [Cp] País de publicación: | England |
| [La] Idioma: | eng |
| [Ab] Resumen: | Activation of vagal afferent sensory C-fibres in the lungs leads to reflex responses that produce many of the symptoms associated with airway allergy. There are two subtypes of respiratory C-fibres whose cell bodies reside within two distinct ganglia, the nodose and jugular, and whose properties allow for differing responses to stimuli. We here used extracellular recording of action potentials in an ex vivo isolated, perfused lung-nerve preparation to study the electrical activity of nodose C-fibres in response to bronchoconstriction. We found that treatment with both histamine and methacholine caused strong increases in tracheal perfusion pressure that were accompanied by action potential discharge in nodose, but not in jugular C-fibres. Both the increase in tracheal perfusion pressure and action potential discharge in response to histamine were significantly reduced by functionally antagonizing the smooth muscle contraction with isoproterenol, or by blocking myosin light chain kinase with ML-7. We further found that pretreatment with AF-353 or 2',3'-O-(2,4,6-Trinitrophenyl)-adenosine-5'-triphosphate (TNP-ATP), structurally distinct P2X3 and P2X2/3 purinoceptor antagonists, blocked the bronchoconstriction-induced nodose C-fibre discharge. Likewise, treatment with the ATPase apyrase, in the presence of the adenosine A1 and A2 receptor antagonists 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) and SCH 58261, blocked the C-fibre response to histamine, without inhibiting the bronchoconstriction. These results suggest that ATP released within the tissues in response to bronchoconstriction plays a pivotal role in the mechanical activation of nodose C-fibres. |
| [Mh] Términos MeSH primario: |
Adenosina Trifosfato/metabolismo
Espasmo Bronquial/inducido químicamente
Nervio Vago/fisiología
|
| [Mh] Términos MeSH secundario: |
Potenciales de Acción
Antagonistas del Receptor de Adenosina A1/farmacología
Antagonistas del Receptor de Adenosina A2/farmacología
Animales
Apirasa
Espasmo Bronquial/metabolismo
Cobayas
Histamina/farmacología
Masculino
Cloruro de Metacolina/farmacología
Ganglio Nudoso/citología
Ganglio Nudoso/fisiología
Antagonistas del Receptor Purinérgico P2X
Receptor de Adenosina A1/metabolismo
Receptores de Adenosina A2/metabolismo
Receptores Purinérgicos P2X/metabolismo
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
Técnicas de Cultivo de Tejidos
|
| [Pt] Tipo de publicación: | JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL |
[Nm] Nombre de substancia:
| 0 (Adenosine A1 Receptor Antagonists); 0 (Adenosine A2 Receptor Antagonists); 0 (Purinergic P2X Receptor Antagonists); 0 (Receptor, Adenosine A1); 0 (Receptors, Adenosine A2); 0 (Receptors, Purinergic P2X); 51-45-6 (Histamine); 56-65-5 (Adenosine Triphosphate); 62-51-1 (Methacholine Chloride); EC 3.6.1.5 (Apyrase) |
| [Em] Mes de ingreso: | 1301 |
| [Cu] Fecha actualización por clase: | 130503 |
| [Lr] Fecha última revisión: | 130503 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Fecha de ingreso para procesamiento: | 120820 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.1113/jphysiol.2012.233460 |
