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[PMID]:29305450
[Au] Autor:Kono K; Tomita T; Futai K; Yamazaki T; Tanaka S; Yoshikawa H; Sugamoto K
[Ad] Dirección:Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan and Department of Orthopaedic Biomaterial Science, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan.
[Ti] Título: three-dimensional kinematics of normal knees during different high-flexion activities.
[So] Fuente:Bone Joint J;100-B(1):50-55, 2018 Jan.
[Is] ISSN:2049-4408
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:AIMS: In Asia and the Middle-East, people often flex their knees deeply in order to perform activities of daily living. The purpose of this study was to investigate the 3D kinematics of normal knees during high-flexion activities. Our hypothesis was that the femorotibial rotation, varus-valgus angle, translations, and kinematic pathway of normal knees during high-flexion activities, varied according to activity. MATERIALS AND METHODS: We investigated the kinematics of eight normal knees in four male volunteers (mean age 41.8 years; 37 to 53) using 2D and 3D registration technique, and modelled the knees with a computer aided design program. Each subject squatted, kneeled, and sat cross-legged. We evaluated the femoral rotation and varus-valgus angle relative to the tibia and anteroposterior translation of the medial and lateral side, using the transepicodylar axis as our femoral reference relative to the perpendicular projection on to the tibial plateau. This method evaluates the femur medially from what has elsewhere been described as the extension facet centre, and differs from the method classically applied. RESULTS: During squatting and kneeling, the knees displayed femoral external rotation. When sitting cross-legged, femurs displayed internal rotation from 10° to 100°. From 100°, femoral external rotation was observed. No significant difference in varus-valgus angle was seen between squatting and kneeling, whereas a varus position was observed from 140° when sitting cross-legged. The measure kinematic pathway using our methodology found during squatting a medial pivoting pattern from 0° to 40° and bicondylar rollback from 40° to 150°. During kneeling, a medial pivot pattern was evident. When sitting cross-legged, a lateral pivot pattern was seen from 0° to 100°, and a medial pivot pattern beyond 100°. CONCLUSION: The kinematics of normal knees during high flexion are variable according to activity. Nevertheless, our study was limited to a small number of male patients using a different technique to report the kinematics than previous publications. Accordingly, caution should be observed in generalizing our findings. Cite this article: 2018;100-B:50-5.
[Mh] Términos MeSH primario: Fenómenos Biomecánicos/fisiología
Articulación de la Rodilla/fisiología
[Mh] Términos MeSH secundario: Actividades Cotidianas
Adulto
Simulación por Computador
Diseño Asistido por Computador
Fluoroscopía
Seres Humanos
Imagen Tridimensional/métodos
Articulación de la Rodilla/diagnóstico por imagen
Masculino
Mediana Edad
Modelos Anatómicos
Rango del Movimiento Articular/fisiología
Rotación
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1801
[Cu] Fecha actualización por clase:180311
[Lr] Fecha última revisión:180311
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:180107
[St] Status:MEDLINE
[do] DOI:10.1302/0301-620X.100B1.BJJ-2017-0553.R2


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[PMID]:29381747
[Au] Autor:Devyatko D; Pastukhov A
[Ad] Dirección:Laboratory for Cognitive Research, National Research University Higher School of Economics, Moscow, Russia.
[Ti] Título:Extrinsic grouping factors in motion-induced blindness.
[So] Fuente:PLoS One;13(1):e0192133, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:We investigated how various grouping factors altered subjective disappearances of the individual targets in the motion-induced blindness display. The latter relies on a moving mask to render highly salient static targets temporarily subjectively invisible. Specifically, we employed two extrinsic grouping factors, the connectedness and the common region, and examined whether their presence would make targets more resilient against the suppression. In addition, we investigated whether the presence of an illusory Kanizsa triangle would affect the suppression of the inducing Pac-Man elements. We quantified the perceptual dynamics using the proportion of the disappearance time (this indicates whether targets became more resilient against the suppression), and the proportion of simultaneous disappearance and reappearance events (characterizes the tendency for the targets to disappear or reappear as a group). We report that a single mask that encompassed all targets (a common region grouping) significantly increased the proportion of simultaneous disappearance and reappearance events, but had no effect on the proportion of the disappearance time. In contrast, a line that connected two targets significantly decreased the total invisibility time, but had no impact on the simultaneity of the disappearance and reappearance events. We found no statistically significant effect of the presence of the illusory Kanizsa triangle on either measure. Finally, we found no interaction either between the common region and the connectedness or between the common region and the presence of the illusory Kanizsa triangle. Our results indicate that extrinsic grouping factors might influence the perception differently than the intrinsic ones and highlight the importance of using several measures to characterize the perceptual dynamics, as various grouping factors might affect it differentially.
[Mh] Términos MeSH primario: Ceguera
Percepción de Movimiento
[Mh] Términos MeSH secundario: Adolescente
Adulto
Femenino
Seres Humanos
Masculino
Adulto Joven
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0192133


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[PMID]:29377950
[Au] Autor:Wang M; Zhou S; Yan W
[Ad] Dirección:College of Meteorology and Oceanography, National University of Defense Technology, Jiangsu Province, PR of China.
[Ti] Título:Blurred image restoration using knife-edge function and optimal window Wiener filtering.
[So] Fuente:PLoS One;13(1):e0191833, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Motion blur in images is usually modeled as the convolution of a point spread function (PSF) and the original image represented as pixel intensities. The knife-edge function can be used to model various types of motion-blurs, and hence it allows for the construction of a PSF and accurate estimation of the degradation function without knowledge of the specific degradation model. This paper addresses the problem of image restoration using a knife-edge function and optimal window Wiener filtering. In the proposed method, we first calculate the motion-blur parameters and construct the optimal window. Then, we use the detected knife-edge function to obtain the system degradation function. Finally, we perform Wiener filtering to obtain the restored image. Experiments show that the restored image has improved resolution and contrast parameters with clear details and no discernible ringing effects.
[Mh] Términos MeSH primario: Aumento de la Imagen/métodos
Modelos Teóricos
Fotografía
[Mh] Términos MeSH secundario: Movimiento (Física)
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:180130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191833


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[PMID]:29366479
[Au] Autor:Xia E; Bhandari A; Shen Y; Zhou X; Sindan N; Xiang J; Guan Y; Yang F; Wang O
[Ad] Dirección:Department of Thyroid & Breast Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, PR China.
[Ti] Título:LncRNA CCND2-AS1 promotes proliferation, migration, and invasion in papillary thyroid carcinoma.
[So] Fuente:Biochem Biophys Res Commun;496(2):628-632, 2018 02 05.
[Is] ISSN:1090-2104
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:In decades, a lot of long non-coding RNAs (LncRNAs) have been proven to exert influences on tumorigenesis in vitro and in vivo. Many lncRNAs have been reported as effective therapeutic targets and biomarkers in various cancers. However, whether LncRNAs are associated with the progression of PTC remains largely unknown. In this study, we measured the expression of CCND2-AS1 in PTC cell lines by quantitative real-time polymerase chain reaction (qRT-PCR).We found that CCND2-AS1 expression was significantly over-expressed in PTC cell lines compared to normal thyroid epithelial cells. Gain-and loss-of-function experiments were performed to investigate the role of CCND2-AS1 in PTC cells. In vitro experiments, we proved that CCND2-AS1 knockdown in TPC1 significantly suppressed cell proliferation, migration, and invasion, while CCND2-AS1 overexpression in BCPAP had the opposite effects. Meanwhile, we also found that CCND2-AS1 could regulate N-cadherin and Vimentin expression, which may influence invasion and migration. Our findings indicate that the lncRNA CCND2-AS1 is a gene associated with PTC and might become a potential therapeutic target.
[Mh] Términos MeSH primario: Carcinoma Papilar/genética
Regulación Neoplásica de la Expresión Génica
Invasividad Neoplásica/genética
ARN Largo no Codificante/genética
Neoplasias de la Tiroides/genética
[Mh] Términos MeSH secundario: Carcinoma Papilar/patología
Línea Celular Tumoral
Movimiento Celular
Proliferación Celular
Seres Humanos
Invasividad Neoplásica/patología
Glándula Tiroides/patología
Neoplasias de la Tiroides/patología
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nombre de substancia:
0 (RNA, Long Noncoding)
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:180126
[St] Status:MEDLINE


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[PMID]:29364932
[Au] Autor:Kang BK; Kim JS; Ryun S; Chung CK
[Ad] Dirección:Human Brain Function Laboratory, Department of Neurosurgery, Seoul National University Hospital, Seoul, South Korea.
[Ti] Título:Prediction of movement intention using connectivity within motor-related network: An electrocorticography study.
[So] Fuente:PLoS One;13(1):e0191480, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Most brain-machine interface (BMI) studies have focused only on the active state of which a BMI user performs specific movement tasks. Therefore, models developed for predicting movements were optimized only for the active state. The models may not be suitable in the idle state during resting. This potential maladaptation could lead to a sudden accident or unintended movement resulting from prediction error. Prediction of movement intention is important to develop a more efficient and reasonable BMI system which could be selectively operated depending on the user's intention. Physical movement is performed through the serial change of brain states: idle, planning, execution, and recovery. The motor networks in the primary motor cortex and the dorsolateral prefrontal cortex are involved in these movement states. Neuronal communication differs between the states. Therefore, connectivity may change depending on the states. In this study, we investigated the temporal dynamics of connectivity in dorsolateral prefrontal cortex and primary motor cortex to predict movement intention. Movement intention was successfully predicted by connectivity dynamics which may reflect changes in movement states. Furthermore, dorsolateral prefrontal cortex is crucial in predicting movement intention to which primary motor cortex contributes. These results suggest that brain connectivity is an excellent approach in predicting movement intention.
[Mh] Términos MeSH primario: Electrocorticografía/métodos
Movimiento
[Mh] Términos MeSH secundario: Adulto
Mapeo Encefálico
Epilepsia/diagnóstico por imagen
Epilepsia/fisiopatología
Femenino
Seres Humanos
Masculino
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:180125
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191480


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[PMID]:28456536
[Au] Autor:More Bayona JA; Karuppannan AK; Barreda DR
[Ad] Dirección:Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
[Ti] Título:Contribution of leukocytes to the induction and resolution of the acute inflammatory response in chickens.
[So] Fuente:Dev Comp Immunol;74:167-177, 2017 Sep.
[Is] ISSN:1879-0089
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:A successful immune response against invading pathogens relies on the efficient activation of host defense mechanisms and a timely return to immune homeostasis. Despite their importance, these mechanisms remain ill-defined in most animal groups. This study focuses on the acute inflammatory response of chickens, important both as an avian model with a unique position in evolution as well as an increasingly notable target of infectious zoonotic diseases. We took advantage of an in vivo self-resolving intra-abdominal challenge model to provide an integrative view of leukocyte responses during the induction and resolution phases of acute inflammation. Our results showed rapid leukocyte infiltration into the abdominal cavity post zymosan challenge (significant increase as early as 4 h), which was dominated by heterophils. Peak leukocyte infiltration and ROS production reached maximum levels at 12 h post challenge, which was significantly earlier than comparative studies in teleost fish and mice. Both heterophils and monocyte/macrophages contributed to ROS production. Local leukocyte infiltration was preceded by an increase in peripheral leukocytes and a drop in the number of bone marrow leukocytes. The proportion of apoptotic leukocytes increased following peak of acute inflammation, rising to significant levels within the abdominal cavity by 48 h, consistent with other indicators for the resolution of inflammation. Importantly, comparison of chicken phagocytic responses with those previously shown in agnathan, teleost and murine models suggested a progressive evolutionary shift towards an increased sensitivity to pro-inflammatory pathogen-derived particles and decreased sensitivity towards homeostatic stimuli. Thus, while significant conservation can be noted across the immune systems of endotherms, this study highlights additional unique features that govern the induction and resolution of acute inflammation in the avian system, which may be relevant to disease susceptibility and performance.
[Mh] Términos MeSH primario: Enfermedades de las Aves/inmunología
Pollos/inmunología
Inflamación/inmunología
Leucocitos/inmunología
Peritoneo/fisiología
Zoonosis/inmunología
[Mh] Términos MeSH secundario: Enfermedad Aguda
Animales
Apoptosis
Evolución Biológica
Movimiento Celular
Proliferación Celular
Peces
Seres Humanos
Inmunidad Innata
Ratones
Fagocitosis
Fisiología Comparada
Especies Reactivas de Oxígeno/metabolismo
Zimosan/inmunología
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Reactive Oxygen Species); 9010-72-4 (Zymosan)
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170501
[St] Status:MEDLINE


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[PMID]:28452702
[Au] Autor:Shin JM; Kang JH; Lee SA; Park IH; Lee HM
[Ad] Dirección:Department of Otorhinolaryngology-Head and Neck Surgery, Seoul, South Korea.
[Ti] Título:Effect of doxycycline on epithelial-mesenchymal transition the p38/Smad pathway in respiratory epithelial cells.
[So] Fuente:Am J Rhinol Allergy;31(2):71-77, 2017 Mar 01.
[Is] ISSN:1945-8932
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:PURPOSE: Doxycycline has antibacterial and anti-inflammatory effects, and it also suppresses collagen biosynthesis. This study aimed to confirm the effects and mechanism of doxycycline on transforming growth factor (TGF) beta 1 induced epithelial-mesenchymal transition and cell migration in A549 and primary nasal epithelial cells. METHODS: A 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide assay and phalloidin-fluorescein isothiocyanate staining were used to evaluate cytotoxicity and cellular morphologic changes. Western blot and immunofluorescence staining were used to determine the expression levels of E-cadherin, vimentin, alpha-smooth muscle actin, fibronectin, phosphorylated Smad2/3, and mitogen-activated protein kinases. Scratch and transwell migration assays were used to assess cellular migration ability. RESULTS: Doxycycline (0-10 µg/mL) had no significant cytotoxic effects in A549 and primary nasal epithelial cells. Increased expression of mesenchymal markers, including vimentin, alpha-smooth muscle actin, and fibronectin in TGF beta 1 induced A549 cells were downregulated by doxycycline treatment. In contrast, E-cadherin expression was upregulated in TGF beta 1 induced A549 cells. An in vitro cell migration assay showed that doxycycline also inhibited the ability of TGF beta 1 induced migration. Doxycycline treatment suppressed the activation of Smad2/3 and p38, whereas its inhibitory effects were similar to each element-specific inhibitor in A549 and primary nasal epithelial cells. CONCLUSION: Doxycycline inhibited TGF beta 1 induced epithelial-to-mesenchymal transition and migration by targeting Smad2/3 and p38 signal pathways in respiratory epithelial cells.
[Mh] Términos MeSH primario: Antineoplásicos/farmacología
Doxiciclina/farmacología
Transición Epitelial-Mesenquimal/efectos de los fármacos
Mucosa Respiratoria/efectos de los fármacos
[Mh] Términos MeSH secundario: Células A549
Cadherinas/metabolismo
Movimiento Celular/efectos de los fármacos
Regulación de la Expresión Génica
Seres Humanos
Sistema de Señalización de MAP Quinasas
Cultivo Primario de Células
Mucosa Respiratoria/patología
Proteína Smad2/metabolismo
Proteína smad3/metabolismo
Factor de Crecimiento Transformador beta/metabolismo
Vimentina/metabolismo
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Antineoplastic Agents); 0 (Cadherins); 0 (Smad2 Protein); 0 (Smad3 Protein); 0 (Transforming Growth Factor beta); 0 (Vimentin); N12000U13O (Doxycycline)
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170429
[St] Status:MEDLINE
[do] DOI:10.2500/ajra.2017.31.4410


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[PMID]:29364828
[Au] Autor:Shetty P; Naik R; Patil A
[Ad] Dirección:Department of Orthodontics and Dentofacial Orthopedics, SDM College of Dental Sciences and Hospital, Sattur, Dharwad, Karnataka 580009, India. pratikshashetty1617@gmail.com.
[Ti] Título:Multipurpose ligation technique for elastomeric chain.
[So] Fuente:J Clin Orthod;51(12):821-822, 2017 Dec.
[Is] ISSN:0022-3875
[Cp] País de publicación:United States
[La] Idioma:eng
[Mh] Términos MeSH primario: Aparatos Ortodóncicos
Técnicas de Movimiento Dental/instrumentación
Técnicas de Movimiento Dental/métodos
[Mh] Términos MeSH secundario: Seres Humanos
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:D
[Da] Fecha de ingreso para procesamiento:180125
[St] Status:MEDLINE


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[PMID]:29270670
[Au] Autor:Lv C; Wang H; Tong Y; Yin H; Wang D; Yan Z; Liang Y; Wu D; Su Q
[Ad] Dirección:Department of General Surgery, Shengjing Hospital Affiliated to China Medical University, Shenyang City, Liaoning Province, 110004, People's Republic of China.
[Ti] Título:The function of BTG3 in colorectal cancer cells and its possible signaling pathway.
[So] Fuente:J Cancer Res Clin Oncol;144(2):295-308, 2018 Feb.
[Is] ISSN:1432-1335
[Cp] País de publicación:Germany
[La] Idioma:eng
[Ab] Resumen:PURPOSE: B-cell translocation gene 3 (BTG3) has been identified as a candidate driver gene for various cancers, but its specific role in colorectal cancer (CRC) is poorly understood. We aimed to investigate the relationship between expression of BTG3 and clinicopathological features and prognosis, as well as to explore the effects and the role of a possible BTG3 molecular mechanism on aggressive colorectal cancer behavior. METHODS: BTG3 expression was assessed by immunohistochemistry (IHC) on specimens from 140 patients with CRC. The association of BTG3 expression with clinicopathological features was examined. To confirm the biological role of BTG3 in CRC, two CRC cell lines expressing BTG3 were used and BTG3 expression was knocked down by shRNA. CCK-8, cell cycle, apoptosis, migration, and invasion assays were performed. The influence of BTG3 knockdown was further investigated by genomic microarray to uncover the potential molecular mechanisms underlying BTG3-mediated CRC development and progression. RESULTS: BTG3 was downregulated in colorectal cancer tissues and positively correlated with pathological classification (p = 0.037), depth of invasion (p = 0.016), distant metastasis (p = 0.024), TNM stage (p = 0.007), and overall survival (OS) and disease-free survival (DFS). BTG3 knockdown promoted cell proliferation, migration, invasion, relieved G2 arrest, and inhibited apoptosis in HCT116 and LoVo cells. A genomic microarray analysis showed that numerous tumor-associated signaling pathways and oncogenes were altered by BTG3 knockdown. At the mRNA level, nine genes referred to the extracellular-regulated kinase/mitogen-activated protein kinase pathway were differentially expressed. Western blotting revealed that BTG3 knockdown upregulated PAK2, RPS6KA5, YWHAB, and signal transducer and activator of transcription (STAT)3 protein levels, but downregulated RAP1A, DUSP6, and STAT1 protein expression, which was consistent with the genomic microarray data. CONCLUSIONS: BTG3 expression might contribute to CRC carcinogenesis. BTG3 knockdown might strengthen the aggressive colorectal cancer behavior.
[Mh] Términos MeSH primario: Neoplasias Colorrectales/metabolismo
Proteínas/metabolismo
[Mh] Términos MeSH secundario: Línea Celular Tumoral
Movimiento Celular/fisiología
Proliferación Celular/fisiología
Neoplasias Colorrectales/genética
Neoplasias Colorrectales/patología
Regulación hacia Abajo
Femenino
Técnicas de Silenciamiento del Gen
Células HCT116
Células HT29
Seres Humanos
Inmunohistoquímica
Masculino
Mediana Edad
Adhesión en Parafina
Proteínas/genética
Transducción de Señales
Células Tumorales Cultivadas
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (BTG3 protein, human); 0 (Proteins)
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171223
[St] Status:MEDLINE
[do] DOI:10.1007/s00432-017-2561-9


  10 / 275838 MEDLINE  
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[PMID]:29442033
[Au] Autor:Xia M; Wei J; Tong K
[Ti] Título:MiR-224 promotes proliferation and migration of gastric cancer cells through targeting PAK4.
[So] Fuente:Pharmazie;71(8):460-464, 2016 Aug 01.
[Is] ISSN:0031-7144
[Cp] País de publicación:Germany
[La] Idioma:eng
[Ab] Resumen:Although recent studies have shown the important role and overexpression of miR-224 in several tumors, its function in gastric cancer has not yet been defined. In the present study, we tried to confirm the result of microRNAs microarray and further investigated the functions of miR-224 in gastric cancer, and tried to find new downstream targets of miR-224. In this study, the level of miR-224 was measured in gastric cancer cells with the normal human gastric epithelial cell. The effects of miR-224 of on proliferation, migration, and target protein expression were evaluated by CCK8 assay, colony assay, transwell migration assay, western blotting. In addition, luciferase reporter plasmid was constructed to demonstrate the direct target of miR-224. Overexpression of miR-224 was detected in the gastric cancer cells, especially in SCG-7901. Exogenous miR-224 expression promoted the proliferation and migration of gastric cells and abrogating expression of miR-224 suppressed proliferation, and migration of SCG-7901 cells in vitro. Luciferase assays revealed that miR-224 directly targeted the 3'UTR of p21-activated kinase 4 (PAK4). The present study provides an experimental foundation for miR-224 as a potential tumor suppressor that may decrease PAK4 expression to inhibit gastric cancer cells and that in the future, targeting of this miRNA may provide a novel strategy for the diagnosis and treatment of patients with this lethal disease.
[Mh] Términos MeSH primario: Movimiento Celular/efectos de los fármacos
Proliferación Celular/efectos de los fármacos
MicroARNs/uso terapéutico
Neoplasias Gástricas/patología
Quinasas p21 Activadas/efectos de los fármacos
[Mh] Términos MeSH secundario: Línea Celular Tumoral
Células Epiteliales/efectos de los fármacos
Regulación Neoplásica de la Expresión Génica
Genes Reporteros/genética
Seres Humanos
MicroARNs/genética
MicroARNs/farmacología
Plásmidos/genética
Regulación hacia Arriba
Cicatrización de Heridas/efectos de los fármacos
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (MIRN224 microRNA, human); 0 (MicroRNAs); EC 2.7.1.11 (PAK4 protein, human); EC 2.7.11.1 (p21-Activated Kinases)
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180307
[Lr] Fecha última revisión:180307
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:180215
[St] Status:MEDLINE
[do] DOI:10.1691/ph.2016.6580



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BIREME/OPS/OMS - Centro Latinoamericano y del Caribe de Información en Ciencias de la Salud