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  1 / 281736 MEDLINE  
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[PMID]:25692336
[Au] Autor:Rachlis B; Ochieng D; Geng E; Rotich E; Ochieng V; Maritim B; Ndege S; Naanyu V; Martin JN; Keter A; Ayuo P; Diero L; Nyambura M; Braitstein P
[Ad] Dirección:*Academic Model Providing Access To Healthcare, Eldoret, Kenya; †Division of Epidemiology, Dalla Lana School of Public Health, University of Toronto, Toronto, Ontario, Canada; ‡Department of Epidemiology and Biostatistics, University of California, San Francisco, San Francisco, CA; §Moi University School of Public Health, Kenya; Departments of ‖Behavioral Sciences; and ¶Medicine, School of Medicine, College of Health Sciences, Moi University, Eldoret, Kenya.
[Ti] Título:Implementation and operational research: evaluating outcomes of patients lost to follow-up in a large comprehensive care treatment program in western Kenya.
[So] Fuente:J Acquir Immune Defic Syndr;68(4):e46-55, 2015 Apr 1.
[Is] ISSN:1944-7884
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:BACKGROUND: Academic Model Providing Access To Healthcare (AMPATH) program provides comprehensive HIV care and treatment services. Approximately, 30% of patients have become lost to follow-up (LTFU). We sought to actively trace and identify outcomes for a sample of these patients. METHODS: LTFU was defined as missing a scheduled visit by ≥3 months. A randomly selected sample of 17% of patients identified as LTFU between January 2009 and June 2011 was generated, with sample stratification on age, antiretroviral therapy (ART) status at last visit, and facility. Chart reviews were conducted followed by active tracing. Tracing was completed by trained HIV-positive outreach workers July 2011 to February 2012. Outcomes were compared between adults and children and by ART status. RESULTS: Of 14,811 LTFU patients, 2540 were randomly selected for tracing (2179 adults, 1071 on ART). The chart reviews indicated that 326 (12.8%) patients were not actually LTFU. Outcomes for 71% of sampled patients were determined including 85% of those physically traced. Of those with known outcomes, 21% had died, whereas 29% had disengaged from care for various reasons. The remaining patients had moved away (n = 458, 25%) or were still receiving HIV care (n = 443 total, 25%). CONCLUSIONS: Our findings demonstrate the feasibility of a large-scale sampling-based approach. A significant proportion of patients were found not to be LTFU, and further, high numbers of patients who were LTFU could not be located. Over a quarter of patients disengaged from care for various reasons including access challenges and familial influences.
[Mh] Términos MeSH primario: Infecciones por VIH/quimioterapia
Perdida de Seguimiento
[Mh] Términos MeSH secundario: Adolescente
Adulto
Niño
Preescolar
Femenino
Humanos
Lactante
Recién Nacido
Kenia
Masculino
Mediana Edad
Adulto Joven
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mes de ingreso:1504
[Cu] Fecha actualización por clase:150516
[Lr] Fecha última revisión:150516
[Sb] Subgrupo de revista:IM; X
[Da] Fecha de ingreso para procesamiento:150219
[St] Status:MEDLINE
[do] DOI:10.1097/QAI.0000000000000492


  2 / 281736 MEDLINE  
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[PMID]:25394191
[Au] Autor:Abler L; Sikkema KJ; Watt MH; Pitpitan EV; Kalichman SC; Skinner D; Pieterse D
[Ad] Dirección:*Duke Global Health Institute, Duke University, Durham, NC; †Department of Psychology and Neuroscience, Duke University, Durham, NC; ‡Division of Global Public Health, Department of Medicine, University of California San Diego, San Diego, CA; §Department of Psychology, University of Connecticut, Storrs, CT; and ‖Unit for Research on Health and Society, Stellenbosch University, Tygerberg, South Africa.
[Ti] Título:Traumatic stress and the mediating role of alcohol use on HIV-related sexual risk behavior: results from a longitudinal cohort of South African women who attend alcohol-serving venues.
[So] Fuente:J Acquir Immune Defic Syndr;68(3):322-8, 2015 Mar 1.
[Is] ISSN:1944-7884
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:BACKGROUND: In South Africa, alcohol contributes to the HIV epidemic, in part, by influencing sexual behaviors. For some, high levels of alcohol consumption may be driven by previous traumatic experiences that result in traumatic stress. The purpose of this study was to quantify the longitudinal association between traumatic stress and unprotected sex among women who attend drinking venues and to assess whether this association was explained by mediation through alcohol use. METHODS: Data were collected in 4 waves over a year from a prospective cohort of 560 women who regularly attended alcohol-serving venues in a Cape Town township. Longitudinal mixed models examined (1) the relationship between traumatic stress and counts of unprotected sex and (2) whether alcohol use mediated the association between traumatic stress and unprotected sex. RESULTS: Most women reported elevated traumatic stress (80%) and hazardous alcohol use (88%) at least once during the study period. In models adjusted for covariates, traumatic stress was associated with unprotected sex (b = 0.28, SE = 0.06, t = 4.82, P < 0.001). In addition, traumatic stress was associated with alcohol use (b = 0.27, SE = 0.02, t = 14.25, P < 0.001) and was also associated with unprotected sex (b = 0.20, SE = 0.06, t = 3.27, P < 0.01) while controlling for alcohol use (b = 0.28, SE = 0.07, t = 4.25, P < 0.001). The test for the mediated effect established that alcohol use was a significant mediator, accounting for 27% of the total effect of traumatic stress on unprotected sex. CONCLUSIONS: These results highlight the need to address traumatic stress among female venue patrons as an important precursor of HIV risk due to alcohol use.
[Mh] Términos MeSH primario: Consumo de Bebidas Alcohólicas
Infecciones por VIH/psicología
Asunción de Riesgos
Estrés Psicológico
Sexo Inseguro/fisiología
[Mh] Términos MeSH secundario: Adolescente
Adulto
Estudios de Cohortes
Femenino
Humanos
Estudios Longitudinales
Mediana Edad
Estudios Prospectivos
Sudáfrica
Adulto Joven
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mes de ingreso:1504
[Cu] Fecha actualización por clase:150516
[Lr] Fecha última revisión:150516
[Sb] Subgrupo de revista:IM; X
[Da] Fecha de ingreso para procesamiento:150214
[St] Status:MEDLINE
[do] DOI:10.1097/QAI.0000000000000433


  3 / 281736 MEDLINE  
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[PMID]:25623449
[Au] Autor:Yang D; Zhao H; Gao G; Wei K; Zhang L; Han N; Xiao J; Li X; Wang F; Liang H; Zhang W; Wu L
[Ad] Dirección:The Infectious Diseases Center, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China....
[Ti] Título:[Relationship between CD4(+) T lymphocyte cell count and the prognosis (including the healing of the incision wound) of HIV/AIDS patients who had undergone surgical operation].
[So] Fuente:Zhonghua Liu Xing Bing Xue Za Zhi;35(12):1333-6, 2014 Dec.
[Is] ISSN:0254-6450
[Cp] País de publicación:China
[La] Idioma:chi
[Ab] Resumen:OBJECTIVE: To explore the relationship between CD4(+) T lymphocyte cell count and prognosis as well as healing of the surgical incision in HIV/AIDS patients who had received operation. METHODS: Data were collected and analysed retrospectively from 234 HIV/AIDS patients hospitalized at the Beijing Ditan hospital who underwent operation between January 2008 and December 2012. Following factors were taken into consideration that including:age, gender, time and where that anti-HIV(+) was diagnosed, CD4(+)T lymphocyte cell count at the time of operation, part of the body that being operated, typology of incision, different levels of healing on the surgical incision, infection at the incision site, post-operative complications and the prognosis, etc. Wilcoxon rank sum test, χ(2) test, Kruskal-Wallis H test and Spearman rank correlation were used for statistical analysis to compare the different levels on healing of the incision in relation to the different CD4(+)T lymphocyte cell counts. Rates of level A healing under different CD4(+)T cell counts were also compared. RESULTS: 1) Among the 234 patients including 125 males and 109 females, the average age was 36.17±11.56 years old. Time after discovery of anti-HIV(+)was between 0 and 204 months. The medium CD4(+)T cell count was 388.5 cell/µl; 23.93% of the patients having CD4(+)T lymphocyte cell counts as <200 cell/µl. 2) 7.26% of the operations were emergent. There were 23 different organs affected at the time of operation, due to 48 different kinds of illness. 21.37% of the operations belonged to class I incision, 49.57% was class II incision and 29.06% was class III incision. 86.32% of the incisions resulted in level A healing, 12.51% resulted in level B and 1.71% in level C. 4.27% of the patients developed post-operative complications. Differences between level A healing and level B or C healing in terms of CD4(+)T lymphocyte cell count were not significant (P > 0.05). There was no statistically significant difference on the CD4(+) T lymphocyte count in patients with or without postoperative complications. Difference of the HIV infection time was also not statistically significant between the two groups of patients. Rate of level A healing for the different CD4(+)T lymphocyte cell count was not significant (P > 0.05). Healing of the incision did not show significant correlation with CD4(+) T lymphocyte cell count, duration of antiretroviral therapy or the time that HIV infection was discovered (P > 0.05). CONCLUSION: As long as both the in/exclusion criteria were strictly followed, prognosis for operation on HIV/AIDS seemed to be generally good. Low CD4(+)T lymphocyte cell count should not be taken as a exclusion criteria for operation on HIV/AIDS patients.
[Mh] Términos MeSH primario: Síndrome de Inmunodeficiencia Adquirida/inmunología
Recuento de Linfocito CD4
Procedimientos Quirúrgicos Operativos
[Mh] Términos MeSH secundario: Adulto
Enfermedades Transmisibles
Femenino
Hospitales
Humanos
Masculino
Mediana Edad
Pronóstico
Estudios Retrospectivos
Linfocitos T
Cicatrización de Heridas
Adulto Joven
[Pt] Tipo de publicación:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Mes de ingreso:1505
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:150127
[St] Status:MEDLINE


  4 / 281736 MEDLINE  
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[PMID]:25623448
[Au] Autor:Zhang G; Gong Y; Wang Q; Liao Q; Yu G; Wang J; Wang K; Yin B; Xiao L; Li Y; Liu Z
[Ad] Dirección:National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China....
[Ti] Título:[Survival analysis on AIDS patients undergoing antiretroviral treatment, Liangshan prefecture, Sichuan province].
[So] Fuente:Zhonghua Liu Xing Bing Xue Za Zhi;35(12):1329-32, 2014 Dec.
[Is] ISSN:0254-6450
[Cp] País de publicación:China
[La] Idioma:chi
[Ab] Resumen:OBJECTIVE: To analyze the survival time and its impact factors among AIDS patients who initially received antiretroviral treatment (ART) in Liangshan prefecture during 2004-2012. METHODS: A retrospective cohort study was conducted to analyze the information of AIDS patients who initially received ART. Data on patients was collected from the Chinese AIDS Antiretroviral Therapy DATAFax Information System. Life table was applied to calculate the survival proportion, and Cox proportion hazard regression model was used to identify impact factors that were related to the time of survival. RESULTS: Among 5 525 AIDS patients who initially received ART, the median age was (34.5± 9.0) year old, with 73.9% being males, 65.8% were infected through injecting drug use, time from HIV tested HIV positive to starting ART was (23.0±20.1) months. 287 cases died of AIDS related diseases, and their median time of receiving ART was (12.7±10.6) months, and 32.8% of them died within the first 6 months of treatment. Cumulative survival rates of the patients who had received ART in 1, 2, 3, 4, 5 years were 97%, 93%, 89%, 88%, 84%, respectively. Results from multivariate Cox regression showed that female patients who received ART were at a lower risk to the death of AIDS related diseases(HR = 0.556, 95%CI:0.367-0.872), when compared to the males. Patients infected with HIV through injecting drug use were at a higher risk to death (HR = 1.569, 95% CI:1.061-2.321) when compared to those who were infected through heterosexual transmission. Patients with baseline CD4(+) T cells counts at <50 cells/mm(3) (HR = 11.996, 95% CI: 6.714-21.435) or 50-200 cells/mm(3) (HR = 2.481, 95%CI:1.620-3.798) were at a higher risk to death than those with CD4(+)T cell counts ≥350 cells/mm(3). Patients without pulmonary tuberculosis were at a lower risk to death(HR = 0.511, 95% CI:0.330-0.791)when compared to those with pulmonary tuberculosis a year before starting the treatment. CONCLUSION: Antiretroviral treatment could prolong the survival time of AIDS patients and with a better rate on survival. Programs on follow-up and CD4(+)T cell counts for AIDS patients should be conducted regularly, as well as timely initiated the antiretroviral therapy.
[Mh] Términos MeSH primario: Síndrome de Inmunodeficiencia Adquirida/quimioterapia
Síndrome de Inmunodeficiencia Adquirida/mortalidad
[Mh] Términos MeSH secundario: Adulto
Fármacos Anti-VIH
Recuento de Linfocito CD4
Estudios de Cohortes
Femenino
Humanos
Masculino
Modelos de Riesgos Proporcionales
Estudios Retrospectivos
Riesgo
Análisis de Supervivencia
Tasa de Supervivencia
Adulto Joven
[Pt] Tipo de publicación:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Anti-HIV Agents)
[Em] Mes de ingreso:1505
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:150127
[St] Status:MEDLINE


  5 / 281736 MEDLINE  
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[PMID]:25623447
[Au] Autor:Yao S; Ye R; Yang Y; Xiang L; Wang J; Du B; Han W; Nie Y; Yang Z; Li W; He N; Duan S
[Ad] Dirección:Dehong Prefecture Center for Disease Control and Prevention, Yunnan 678400, China....
[Ti] Título:[Proportion and related influencing factors of HIV-infected individuals that rejecting the antiretroviral therapy among all the HIV infections, Dehong prefecture, Yunnan province].
[So] Fuente:Zhonghua Liu Xing Bing Xue Za Zhi;35(12):1324-8, 2014 Dec.
[Is] ISSN:0254-6450
[Cp] País de publicación:China
[La] Idioma:chi
[Ab] Resumen:OBJECTIVE: To examine the proportion and influencing factors on HIV-infected individuals who rejecting the antiretroviral therapy among all the HIV positives, in Dehong prefecture, Yunnan province. METHODS: A cross-sectional analysis was conducted on all the local HIV-infected survivals aged over 16 year old who refused to receive antiretroviral therapy (ART) by the end of 2013 in Dehong prefecture. RESULTS: The proportion of those rejecting the ART among HIV-infected survivals and aged over 16 years old in Dehong prefecture, was 7.4% (605/8 136). Factors related to the 'rejection' among the 605 refusals would include: being male (72.9%), aged 31-45 years (57.2%), peasants (75.4%), married (52.2%), with minor ethnicity (41.3%), illiterate or only having primary school education (58.7%), infected through sexual contacts (61.2%), and with CD4(+)T cell counts >350 cells/mm(3) (66.6%). Data from the multiple logistic regression analysis indicated that rejecting the ART was significantly associated with areas, gender, age, ethnicity and CD4(+)T cell counts of the HIV patients. Those who were from Yingjiang county, female, aged 31-45 years old had lower proportions of ART refusals than those who were from Ruili city, male, aged ≤30 year old. Those who were of Dai minority and had no records on CD4(+)T cell counts, had higher proportions of ART refusals than those who were of Han ethnicity and had CD4(+)T cell counts ≤350 cells/mm(3). Reasons for the 605 HIV-infected patients with rejection to the ART would include fear of disclosure of HIV infection status (84, 13.9%), misunderstandings of the effectiveness and side effects of ART (111, 18.3%), self-realized wellness(340, 56.2%) and others (70, 11.6%). Of them, reasons for the 181 patients with CD4(+)T cell counts ≤350 cells/mm(3) that rejecting ART would include fearfulness on the disclosure of HIV infection status(40, 22.1%), misunderstandings of the effectiveness and side effects of ART (36, 19.9%), self-realized wellness (84, 46.4%) and others (21, 11.6%). Among those who rejected ART, reasons for that would vary by areas, gender, age, marital status and routes of HIV transmission, according to the results from Chi-squared tests. CONCLUSION: A substantial proportion of HIV-infected individuals rejected ART in Dehong prefecture of Yunnan province. It was urgently needed to enhance health education programs of ART tailored for those HIV-infected patients, according to different characteristics and reasons for rejection, so as to promote the ART in this prefecture.
[Mh] Términos MeSH primario: Antirretrovirales/uso terapéutico
Infecciones por VIH/quimioterapia
Negativa del Paciente al Tratamiento
[Mh] Términos MeSH secundario: Adulto
China
Estudios Transversales
Grupos Étnicos
Femenino
Educación en Salud
Necesidades y Demandas de Servicios de Salud
Humanos
Masculino
Estado Civil
Matrimonio
Mediana Edad
Grupos Minoritarios
Conducta Sexual
[Pt] Tipo de publicación:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Anti-Retroviral Agents)
[Em] Mes de ingreso:1505
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:150127
[St] Status:MEDLINE


  6 / 281736 MEDLINE  
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[PMID]:25623446
[Au] Autor:Qiu T; Ding P; Liu X; Xu J; Guo H; Fu G; Xu X; Huan X
[Ad] Dirección:Department of HIV/STD Prevention and Control, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China....
[Ti] Título:[Epidemiological characteristics of HIV/AIDS patients newly received highly active antiretroviral therapy during 2005-2013 in Jiangsu province].
[So] Fuente:Zhonghua Liu Xing Bing Xue Za Zhi;35(12):1320-3, 2014 Dec.
[Is] ISSN:0254-6450
[Cp] País de publicación:China
[La] Idioma:chi
[Ab] Resumen:OBJECTIVE: To analyze the epidemiological characteristics of the HIV/AIDS patients newly received highly active antiretroviral therapy (HAART) during 2005-2013 in Jiangsu province. METHODS: According to the baseline data of HIV/AIDS patients newly received HAART during 2005-2013, an Excel database was established and statistical analysis was performed using SPSS 16.0 software. RESULTS: There were 5 788 HIV/AIDS patients newly received HAART during 2005-2013 and the number increased annually. 79.7% of these patients were from the local province, with the ratio of male to female as 4.69 : 1, mean age as 39.9 years old, 55.8% of them married, 52.0% of them were treated at CDC. 91.9% of them were infected through sexual contacts. Mean baseline CD4(+)T cell count of cases was 187 cells/µl. Median of the interval between the time of HIV confirmation and HAART initiation was 4.0 months. Proportion of the HIV/AIDS patients that were being male, elderly, unmarried, not local resident, treated in hospitals and with higher baseline CD4(+)T count etc. showed a trend of increase. CONCLUSION: HIV/AIDS patients had the opportunity to start the HAART at early stage of the disease. However, many of them started the treafment late as the results of being diagnosed late. Programs related to both earlier detection and diagnosis on HIV cases should be strengthened.
[Mh] Términos MeSH primario: Síndrome de Inmunodeficiencia Adquirida/quimioterapia
Síndrome de Inmunodeficiencia Adquirida/epidemiología
Terapia Antirretroviral Altamente Activa
[Mh] Términos MeSH secundario: Adulto
Recuento de Linfocito CD4
China/epidemiología
Enfermedades Transmisibles
Femenino
Infecciones por VIH
Humanos
Masculino
[Pt] Tipo de publicación:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Mes de ingreso:1505
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:150127
[St] Status:MEDLINE


  7 / 281736 MEDLINE  
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[PMID]:25392219
[Au] Autor:Woodward CL; Cheng SN; Jensen GJ
[Ad] Dirección:Division of Biology, California Institute of Technology, Pasadena, California, USA.
[Ti] Título:Electron cryotomography studies of maturing HIV-1 particles reveal the assembly pathway of the viral core.
[So] Fuente:J Virol;89(2):1267-77, 2015 Jan 15.
[Is] ISSN:1098-5514
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:UNLABELLED: To better characterize the assembly of the HIV-1 core, we have used electron cryotomography (ECT) to image infected cells and the viral particles cryopreserved next to them. We observed progressive stages of virus assembly and egress, including flower-like flat Gag lattice assemblies, hemispherical budding profiles, and virus buds linked to the plasma membrane via a thin membrane neck. The population of budded viral particles contains immature, maturation-intermediate, and mature core morphologies. Structural characteristics of the maturation intermediates suggest that the core assembly pathway involves the formation of a CA sheet that associates with the condensed ribonucleoprotein (RNP) complex. Our analysis also reveals a correlation between RNP localization within the viral particle and the formation of conical cores, suggesting that the RNP helps drive conical core assembly. Our findings support an assembly pathway for the HIV-1 core that begins with a small CA sheet that associates with the RNP to form the core base, followed by polymerization of the CA sheet along one side of the conical core toward the tip, and then closure around the body of the cone. IMPORTANCE: During HIV-1 assembly and release, the Gag polyprotein is organized into a signature hexagonal lattice, termed the immature lattice. To become infectious, the newly budded virus must disassemble the immature lattice by proteolyzing Gag and then reassemble the key proteolytic product, the structural protein p24 (CA), into a distinct, mature hexagonal lattice during a process termed maturation. The mature HIV-1 virus contains a conical capsid that encloses the condensed viral genome at its wide base. Mutations or small molecules that interfere with viral maturation also disrupt viral infectivity. Little is known about the assembly pathway that results in the conical core and genome encapsidation. Here, we have used electron cryotomography to structurally characterize HIV-1 particles that are actively maturing. Based on the morphologies of core assembly intermediates, we propose that CA forms a sheet-like structure that associates with the condensed viral genome to produce the mature infectious conical core.
[Mh] Términos MeSH primario: Microscopía por Crioelectrón
Tomografía con Microscopio Electrónico
VIH-1/fisiología
VIH-1/ultraestructura
Ensamble de Virus
[Mh] Términos MeSH secundario: Membrana Celular/virología
Células Cultivadas
Células Endoteliales/virología
Humanos
Virión/ultraestructura
Liberación del Virus
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mes de ingreso:1504
[Cu] Fecha actualización por clase:150516
[Lr] Fecha última revisión:150516
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:150105
[St] Status:MEDLINE
[do] DOI:10.1128/JVI.02997-14


  8 / 281736 MEDLINE  
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[PMID]:25518861
[Au] Autor:Bhattacharya A; Alam SL; Fricke T; Zadrozny K; Sedzicki J; Taylor AB; Demeler B; Pornillos O; Ganser-Pornillos BK; Diaz-Griffero F; Ivanov DN; Yeager M
[Ad] Dirección:Department of Biochemistry and Cancer Therapy and Research Center, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229;...
[Ti] Título:Structural basis of HIV-1 capsid recognition by PF74 and CPSF6.
[So] Fuente:Proc Natl Acad Sci U S A;111(52):18625-30, 2014 Dec 30.
[Is] ISSN:1091-6490
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Upon infection of susceptible cells by HIV-1, the conical capsid formed by ∼250 hexamers and 12 pentamers of the CA protein is delivered to the cytoplasm. The capsid shields the RNA genome and proteins required for reverse transcription. In addition, the surface of the capsid mediates numerous host-virus interactions, which either promote infection or enable viral restriction by innate immune responses. In the intact capsid, there is an intermolecular interface between the N-terminal domain (NTD) of one subunit and the C-terminal domain (CTD) of the adjacent subunit within the same hexameric ring. The NTD-CTD interface is critical for capsid assembly, both as an architectural element of the CA hexamer and pentamer and as a mechanistic element for generating lattice curvature. Here we report biochemical experiments showing that PF-3450074 (PF74), a drug that inhibits HIV-1 infection, as well as host proteins cleavage and polyadenylation specific factor 6 (CPSF6) and nucleoporin 153 kDa (NUP153), bind to the CA hexamer with at least 10-fold higher affinities compared with nonassembled CA or isolated CA domains. The crystal structure of PF74 in complex with the CA hexamer reveals that PF74 binds in a preformed pocket encompassing the NTD-CTD interface, suggesting that the principal inhibitory target of PF74 is the assembled capsid. Likewise, CPSF6 binds in the same pocket. Given that the NTD-CTD interface is a specific molecular signature of assembled hexamers in the capsid, binding of NUP153 at this site suggests that key features of capsid architecture remain intact upon delivery of the preintegration complex to the nucleus.
[Mh] Términos MeSH primario: Cápsida/química
VIH-1/química
Indoles/química
Fenilalanina/análogos & derivados
Factores de Escisión y Poliadenilación de ARNm/química
[Mh] Términos MeSH secundario: Cápsida/metabolismo
Cristalografía por Rayos X
Infecciones por VIH
VIH-1/metabolismo
Indoles/farmacología
Proteínas de Complejo Poro Nuclear/química
Proteínas de Complejo Poro Nuclear/metabolismo
Fenilalanina/química
Fenilalanina/farmacología
Unión Proteica
Factores de Escisión y Poliadenilación de ARNm/metabolismo
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nombre de substancia:
0 (Indoles); 0 (NUP153 protein, human); 0 (Nuclear Pore Complex Proteins); 0 (PF-3450074); 0 (cleavage factor Im, human); 0 (mRNA Cleavage and Polyadenylation Factors); 47E5O17Y3R (Phenylalanine)
[Em] Mes de ingreso:1504
[Cu] Fecha actualización por clase:150516
[Lr] Fecha última revisión:150516
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:141231
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.1419945112


  9 / 281736 MEDLINE  
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[PMID]:25216073
[Au] Autor:Azbel L; Wickersham JA; Grishaev Y; Dvoryak S; Altice FL
[Ad] Dirección:Ukrainian Institute on Public Health Policy, Kyiv, Ukraine; Department of Internal Medicine, Section of Infectious Diseases, AIDS Program, Yale University School of Medicine, New Haven, CT, USA; lyuba.azbel@yale.edu....
[Ti] Título:Correlates of HIV infection and being unaware of HIV status among soon-to-be-released Ukrainian prisoners.
[So] Fuente:J Int AIDS Soc;17:19005, 2014.
[Is] ISSN:1758-2652
[Cp] País de publicación:Switzerland
[La] Idioma:eng
[Ab] Resumen:INTRODUCTION: Prisoners bear a disproportionate burden of Ukraine's volatile and transitional HIV epidemic, yet little is known in Eastern Europe about HIV testing, treatment and HIV-related risk among prisoners. METHODS: A nationally representative biobehavioural health survey linked with serological testing was conducted among soon-to-be released prisoners in 13 Ukrainian prisons from June to November 2011. RESULTS: Among 402 participants, 78 (19.4%) tested HIV seropositive of whom 38 (50.7%) were previously unaware of their HIV status. Independent correlates of HIV infection included drug injection (AOR=4.26; 95% CI: 2.23-8.15), female gender (AOR=2.00; 95% CI: 1.06-3.78), previous incarceration (AOR=1.99; 95% CI: 1.07-3.70) and being from Southern Ukraine (AOR=5.46; 95% CI: 2.21-13.46). Those aware of being HIV-positive reported significantly more pre-incarceration sex- and drug-related HIV risk behaviours than those who were unaware. CONCLUSIONS: Routine rather than risk-based HIV testing and expansion of opioid substitution and antiretroviral therapy among prisoners is urgently needed to reduce HIV transmission in volatile transitional HIV epidemics.
[Mh] Términos MeSH primario: Infecciones por VIH/diagnóstico
Infecciones por VIH/epidemiología
Prisioneros
[Mh] Términos MeSH secundario: Adolescente
Adulto
Anciano
Estudios Transversales
Femenino
Humanos
Masculino
Mediana Edad
Factores de Riesgo
Ucrania/epidemiología
Adulto Joven
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mes de ingreso:1503
[Cu] Fecha actualización por clase:150516
[Lr] Fecha última revisión:150516
[Sb] Subgrupo de revista:IM; X
[Da] Fecha de ingreso para procesamiento:140913
[St] Status:MEDLINE
[do] DOI:10.7448/IAS.17.1.19005


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[PMID]:25066863
[Au] Autor:Goodman-Meza D; Pitpitan EV; Semple SJ; Wagner KD; Chavarin CV; Strathdee SA; Patterson TL
[Ad] Dirección:Division of Global Public Health, Department of Medicine, University of California at San Diego, La Jolla, California.
[Ti] Título:Hazardous drinking and HIV-risk-related behavior among male clients of female sex workers in Tijuana, Mexico.
[So] Fuente:Am J Addict;23(5):502-9, 2014 Sep-Oct.
[Is] ISSN:1521-0391
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:BACKGROUND AND OBJECTIVES: Male clients of female sex workers (FSWs) are at high risk for HIV. Whereas the HIV risks of alcohol use are well understood, less is known about hazardous alcohol use among male clients of FSWs, particularly in Mexico. We sought to identify risk factors for hazardous alcohol use and test associations between hazardous alcohol use and HIV risk behavior among male clients in Tijuana. METHOD: Male clients of FSWs in Tijuana (n = 400) completed a quantitative interview in 2008. The AUDIT was used to characterize hazardous alcohol use. Multivariate logistic regression was used to determine independent associations of demographic and HIV risk variables with hazardous alcohol use (vs. non-hazardous). RESULTS: Forty percent of our sample met criteria for hazardous alcohol use. Variables independently associated with hazardous drinking were reporting any sexually transmitted infection (STI), having sex with a FSW while under the influence of alcohol, being younger than 36 years of age, living in Tijuana, and ever having been jailed. Hazardous drinkers were less likely ever to have been deported or to have shared injection drugs. DISCUSSION AND CONCLUSIONS: Hazardous alcohol use is associated with HIV risk, including engaging in sex with FSWs while intoxicated and having an STI among male clients of FSWs in Tijuana. SCIENTIFIC SIGNIFICANCE: We systematically described patterns and correlates of hazardous alcohol use among male clients of FSWs in Tijuana, Mexico. The results suggest that HIV/STI risk reduction interventions must target hazardous alcohol users, and be tailored to address alcohol use.
[Mh] Términos MeSH primario: Consumo de Bebidas Alcohólicas/psicología
Infecciones por VIH/psicología
Trabajadores Sexuales
Sexo Inseguro/psicología
[Mh] Términos MeSH secundario: Adulto
Consumo de Bebidas Alcohólicas/epidemiología
Estudios Transversales
Infecciones por VIH/epidemiología
Encuestas Epidemiológicas
Humanos
Masculino
México/epidemiología
Factores de Riesgo
Enfermedades de Transmisión Sexual/epidemiología
Enfermedades de Transmisión Sexual/psicología
Adulto Joven
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL
[Em] Mes de ingreso:1505
[Cu] Fecha actualización por clase:150516
[Lr] Fecha última revisión:150516
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:140819
[St] Status:MEDLINE
[do] DOI:10.1111/j.1521-0391.2014.12138.x



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