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  1 / 167320 MEDLINE  
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[PMID]:29278028
[Au] Autor:Borda B; Németh T; Ottlakan A; Keresztes C; Kemény É; Lázár G
[Ad] Dirección:1 Faculty of Medicine, Department of Surgery, University of Szeged , Szeged, Hungary.
[Ti] Título:Post-transplantation morphological and functional changes in kidneys from expanded criteria donors.
[So] Fuente:Physiol Int;104(4):329-333, 2017 Dec 01.
[Is] ISSN:2498-602X
[Cp] País de publicación:Hungary
[La] Idioma:eng
[Ab] Resumen:Introduction Despite an increase in the number of cadaver donors and overall organ transplantations, the dramatic increase in the waiting list makes it necessary to reconsider donor criteria. The authors wanted to examine whether differences could exist in the function and/or morphology of transplanted kidneys originated from expanded criteria donors (ECDs) and ideal donors 1 and 5 years after transplantation. Methods Kidney function and histopathologic findings were analyzed and compared 1 and 5 years after transplantation in 97 patients having ECD kidneys and in 178 patients who received ideal donor kidneys (IDK). Results Serum creatinine level was significantly higher (p = 0.001) and estimated glomerular filtration rate was significantly lower (p = 0.003) in patients having ECD kidneys as compared with those with IDK 5 years after transplantation. Morphological changes in the transplanted kidneys, such as tubulitis (p = 0.025) and interstitial inflammation (p = 0.002), were significantly more frequently present in patients with ECD kidneys than in those with IDK 1 year after transplantation. Conclusion Despite an absence of differences in kidney function 1 year after kidney transplantation between patients having ECD and IDK, morphological differences in the transplanted kidneys can be detected between the two groups of patients.
[Mh] Términos MeSH primario: Supervivencia de Injerto/fisiología
Fallo Renal Crónico/patología
Fallo Renal Crónico/fisiopatología
Trasplante de Riñón
Riñón/patología
Riñón/cirugía
Donantes de Tejidos
[Mh] Términos MeSH secundario: Adulto
Anciano
Femenino
Tasa de Filtración Glomerular
Seres Humanos
Fallo Renal Crónico/cirugía
Masculino
Mediana Edad
Tamaño de los Órganos
Obtención de Tejidos y Órganos/métodos
Resultado del Tratamiento
[Pt] Tipo de publicación:CLINICAL TRIAL; JOURNAL ARTICLE
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171227
[St] Status:MEDLINE
[do] DOI:10.1556/2060.104.2017.4.4


  2 / 167320 MEDLINE  
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[PMID]:29211700
[Au] Autor:Kim MJ; Kim JH; Jeon HS; Wee WR; Hyon JY
[Ad] Dirección:*Department of Ophthalmology, Seoul National University College of Medicine, Seoul, Korea; and†Department of Ophthalmology, Seoul National University Bundang Hospital, Seongnam, Korea.
[Ti] Título:Effect of Histocompatibility Y Antigen Matching on Graft Survival in Primary Penetrating Keratoplasty.
[So] Fuente:Cornea;37(1):33-38, 2018 Jan.
[Is] ISSN:1536-4798
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:PURPOSE: To investigate the influence of histocompatibility Y (H-Y) antigen matching on corneal graft survival in primary penetrating keratoplasty (PK). METHODS: Medical records of patients who underwent primary PK at Seoul National University Bundang Hospital between June 2005 and October 2015 were retrospectively analyzed. The eyes were classified into 2 groups: H-Y-compatible (115 eyes) and H-Y-incompatible (23 eyes). The H-Y-compatible group included donor/recipient combinations of male/male (57 eyes), female/male (44 eyes), and female/female (14 eyes). The H-Y-incompatible group included the male/female (23 eyes) combination alone. A subgroup analysis of low- and high-risk patients according to preoperative diagnoses was also performed. Survival analysis was conducted using the Kaplan-Meier method; differences between groups were assessed with a log-rank test. RESULTS: A total of 138 eyes from 136 patients (age: 58 ± 18 years) were enrolled. Rejection-free graft survival and graft survival were not significantly different between H-Y-compatible and H-Y-incompatible groups (χ = 0.4, P = 0.548; χ = 1.9; P = 0.17, respectively). Preoperative diagnoses of high-risk cases included those with corneal perforation or thinning (8.7%) and infectious keratitis (7.2%). Low-risk cases included corneal opacity (50.0%), bullous keratopathy (25.4%), keratoconus (5.8%), and corneal dystrophy (2.9%). In the high-risk group, rejection-free graft survival rate was significantly higher in the H-Y-compatible group (χ = 3.9, P = 0.049). CONCLUSIONS: H-Y antigen matching does not influence graft rejection and failure in cases of primary PK. However, matching the H-Y antigen could help reduce graft rejection, especially in preoperatively high-risk patients.
[Mh] Términos MeSH primario: Enfermedades de la Córnea/cirugía
Supervivencia de Injerto/inmunología
Antígeno H-Y/inmunología
Queratoplastia Penetrante
[Mh] Términos MeSH secundario: Anciano
Aloinjertos
Femenino
Rechazo de Injerto/diagnóstico
Prueba de Histocompatibilidad
Seres Humanos
Masculino
Mediana Edad
Estudios Retrospectivos
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (H-Y Antigen)
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171207
[St] Status:MEDLINE
[do] DOI:10.1097/ICO.0000000000001394


  3 / 167320 MEDLINE  
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[PMID]:28470444
[Au] Autor:Lim PN; Feng J; Wang Z; Chong M; Konishi T; Tan LG; Chan J; Thian ES
[Ad] Dirección:Department of Mechanical Engineering, National University of Singapore, Singapore, 117 576, Singapore.
[Ti] Título:In-vivo evaluation of subcutaneously implanted cell-loaded apatite microcarriers for osteogenic potency.
[So] Fuente:J Mater Sci Mater Med;28(6):86, 2017 Jun.
[Is] ISSN:1573-4838
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Cell-loaded apatite microcarriers present as potential scaffolds for direct in-vivo delivery of cells post-expansion to promote bone regeneration. The objective of this study was to evaluate the osteogenic potency of human foetal mesenchymal stem cells (hfMSC)-loaded apatite microcarriers when implanted subcutaneously in a mouse model. This was done by examining for ectopic bone formation at 2 weeks, 1 month and 2 months, which were intended to coincide with the inflammation, healing and remodelling phases, respectively. Three histological examinations including haematoxylin and eosin staining to examine general tissue morphology, Masson's trichrome staining to identify tissue type, and Von Kossa staining to examine extent of tissue mineralisation were performed. In addition, immunohistochemistry assay of osteopontin was conducted to confirm active bone formation by the seeded hfMSCs. Results showed a high level of tissue organisation and new bone formation, with active bone remodelling being observed at the end of 2 months, and an increase in tissue density, organisation, and mineralisation could also be observed for hfMSC-loaded apatite microcarriers. Various cell morphology resembling that of osteoblasts and osteoclasts could be seen on the surfaces of the hfMSC-loaded apatite microcarriers, with presence of woven bone tissue formation being observed at the intergranular space. These observations were consistent with evidence of ectopic bone formation, which were absent in group containing apatite microcarriers only. Overall, results suggested that hfMSC-loaded apatite microcarriers retained their osteogenic potency after implantation, and provided an effective platform for bone tissue regeneration.
[Mh] Términos MeSH primario: Apatitas/química
Trasplante de Células Madre Mesenquimatosas/métodos
Células del Estroma Mesenquimatoso/fisiología
Osteogénesis/fisiología
[Mh] Términos MeSH secundario: Animales
Diferenciación Celular
Seres Humanos
Ensayo de Materiales
Ratones
Ingeniería de Tejidos/métodos
Andamios del Tejido
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Apatites)
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180309
[Lr] Fecha última revisión:180309
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s10856-017-5897-4


  4 / 167320 MEDLINE  
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[PMID]:29211672
[Au] Autor:Stevens DL; Bryant AE
[Ad] Dirección:From the Veterans Affairs Medical Center, Boise, ID; and the University of Washington School of Medicine, Seattle.
[Ti] Título:Necrotizing Soft-Tissue Infections.
[So] Fuente:N Engl J Med;377(23):2253-2265, 2017 Dec 07.
[Is] ISSN:1533-4406
[Cp] País de publicación:United States
[La] Idioma:eng
[Mh] Términos MeSH primario: Antibacterianos/uso terapéutico
Desbridamiento
Fascitis Necrotizante
[Mh] Términos MeSH secundario: Antiinflamatorios no Esteroideos/efectos adversos
Biomarcadores/sangre
Biopsia
Proteína C-Reactiva/análisis
Enfermedad Crítica
Fascitis Necrotizante/diagnóstico
Fascitis Necrotizante/etiología
Fascitis Necrotizante/patología
Fascitis Necrotizante/terapia
Gangrena Gaseosa
Seres Humanos
Oxigenación Hiperbárica
Inmunoglobulinas Intravenosas/uso terapéutico
Infecciones de los Tejidos Blandos
Infecciones Estreptocócicas/inducido químicamente
Streptococcus pyogenes
[Pt] Tipo de publicación:JOURNAL ARTICLE; REVIEW
[Nm] Nombre de substancia:
0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Biomarkers); 0 (Immunoglobulins, Intravenous); 9007-41-4 (C-Reactive Protein)
[Em] Mes de ingreso:1712
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:171207
[St] Status:MEDLINE
[do] DOI:10.1056/NEJMra1600673


  5 / 167320 MEDLINE  
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[PMID]:29205969
[Au] Autor:Zhang W
[Ad] Dirección:Department of Forensic Medicine, National Police University of China, Shenyang 110035, China.
[Ti] Título:[Forensic Analysis of the Characteristics of Pelvic Fracture in 65 Road Traffic Accident Death Cases].
[So] Fuente:Fa Yi Xue Za Zhi;32(6):428-430, 2016 Dec.
[Is] ISSN:1004-5619
[Cp] País de publicación:China
[La] Idioma:chi
[Ab] Resumen:OBJECTIVES: To analyze the characteristics and mechanisms of pelvic fractures in the cases of road traffic accident deaths. METHODS: Total 65 cases of road traffic accident deaths with pelvic fracture were collected, and the sites, characteristics and injury mechanisms of pelvic fracture were statistically analyzed. RESULTS: Among the 65 cases of pelvic fracture, 38 cases of dislocation of sacroiliac joint were found, and most combined with pubis symphysis separation or fracture of pubis. In the fractures of pubis, ischium and acetabulum, linear fractures were most common, while comminuted fractures were most common in sacrum and coccyx fractures. There were 54 cases combined with pelvic soft tissue injury, and 8 cases with pelvic organ injury and 44 cases with abdominal organ injury. In the types of pelvic ring injury, 32 cases were separation, 49.32%, followed by compression, 26.15% and only one case was verticality, 1.54%. CONCLUSIONS: Detailed and comprehensive examination of the body and determination of the pelvic fracture type contribute to analyze the mechanisms of injury.
[Mh] Términos MeSH primario: Accidentes de Tránsito
Fracturas Óseas/diagnóstico
Huesos Pélvicos/lesiones
[Mh] Términos MeSH secundario: Acetábulo/lesiones
Muerte
Patologia Forense
Fracturas Conminutas/diagnóstico
Seres Humanos
Isquion/lesiones
Traumatismos de los Tejidos Blandos/diagnóstico
Fracturas de la Columna Vertebral/diagnóstico
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1802
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:171206
[St] Status:MEDLINE
[do] DOI:10.3969/j.issn.1004-5619.2016.06.008


  6 / 167320 MEDLINE  
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[PMID]:28954302
[Au] Autor:Fidler MM; Reulen RC; Winter DL; Allodji RS; Bagnasco F; Bárdi E; Bautz A; Bright CJ; Byrne J; Feijen EAM; Garwicz S; Grabow D; Gudmundsdottir T; Guha J; Haddy N; Jankovic M; Kaatsch P; Kaiser M; Kuonen R; Linge H; Maule M; Merletti F; Øfstaas H; Ronckers CM; Skinner R; Teepen J; Terenziani M; Vu-Bezin G; Wesenberg F; Wiebe T; Jakab Z; Haupt R; Lähteenmäki P; Zaletel LZ; Kuehni CE; Winther JF; de Vathaire F; Kremer LC; Hjorth L; Hawkins MM
[Ad] Dirección:Centre for Childhood Cancer Survivor Studies, Institute of Applied Health Research, University of Birmingham, Birmingham, UK; Section of Cancer Surveillance, International Agency for Research on Cancer, Lyon, France; Cancer and Radiation Team, U1018 INSERM, Villejuif, France; Epidemiology and Biosta
[Ti] Título:Risk of Subsequent Bone Cancers Among 69 460 Five-Year Survivors of Childhood and Adolescent Cancer in Europe.
[So] Fuente:J Natl Cancer Inst;110(2), 2018 Feb 01.
[Is] ISSN:1460-2105
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Introduction: We investigate the risks of subsequent primary bone cancers after childhood and adolescent cancer in 12 European countries. For the first time, we satisfactorily address the risks beyond 40 years from diagnosis and beyond 40 years of age among all survivors. Methods: This largest-ever assembled cohort comprises 69 460 five-year survivors of cancer diagnosed before age 20 years. Standardized incidence ratios, absolute excess risks, and multivariable-adjusted relative risks and relative excess risks were calculated. All statistical tests were two-sided. Results: Overall, survivors were 21.65 times (95% confidence interval = 18.97 to 24.60 times) more likely to be diagnosed with a subsequent primary bone cancer than expected from the general population. The greatest excess numbers of bone cancers were observed after retinoblastoma, bone sarcoma, and soft tissue sarcoma. The excess number of bone cancers declined linearly with both years since diagnosis and attained age (all P < .05). Beyond 40 years from diagnosis and age 40 years, there were at most 0.45 excess bone cancers among all survivors per 10 000 person-years at risk; beyond 30 years from diagnosis and age 30 years, there were at most 5.02 excess bone cancers after each of retinoblastoma, bone sarcoma, and soft tissue sarcoma, per 10 000 person-years at risk. Conclusions: For all survivors combined and the cancer groups with the greatest excess number of bone cancers, the excess numbers observed declined with both age and years from diagnosis. These results provide novel, reliable, and unbiased information about risks and risk factors among long-term survivors of childhood and adolescent cancer.
[Mh] Términos MeSH primario: Neoplasias Óseas/epidemiología
Neoplasias Primarias Secundarias/epidemiología
Sobrevivientes/estadística & datos numéricos
[Mh] Términos MeSH secundario: Adolescente
Adulto
Niño
Estudios de Cohortes
Europa (Continente)/epidemiología
Femenino
Estudios de Seguimiento
Seres Humanos
Masculino
Osteosarcoma/epidemiología
Retinoblastoma/epidemiología
Sarcoma/epidemiología
Adulto Joven
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1710
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170928
[St] Status:MEDLINE
[do] DOI:10.1093/jnci/djx165


  7 / 167320 MEDLINE  
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[PMID]:28463576
[Au] Autor:Li K; Zhang C; Qiu L; Gao L; Zhang X
[Ad] Dirección:Tianjin Key Laboratory of Design and Intelligent Control of the Advanced Mechatronical System, School of Mechanical Engineering, Tianjin University of Technology , Tianjin, China .
[Ti] Título:Advances in Application of Mechanical Stimuli in Bioreactors for Cartilage Tissue Engineering.
[So] Fuente:Tissue Eng Part B Rev;23(4):399-411, 2017 Aug.
[Is] ISSN:1937-3376
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Articular cartilage (AC) is the weight-bearing tissue in diarthroses. It lacks the capacity for self-healing once there are injuries or diseases due to its avascularity. With the development of tissue engineering, repairing cartilage defects through transplantation of engineered cartilage that closely matches properties of native cartilage has become a new option for curing cartilage diseases. The main hurdle for clinical application of engineered cartilage is how to develop functional cartilage constructs for mass production in a credible way. Recently, impressive hyaline cartilage that may have the potential to provide capabilities for treating large cartilage lesions in the future has been produced in laboratories. The key to functional cartilage construction in vitro is to identify appropriate mechanical stimuli. First, they should ensure the function of metabolism because mechanical stimuli play the role of blood vessels in the metabolism of AC, for example, acquiring nutrition and removing wastes. Second, they should mimic the movement of synovial joints and produce phenotypically correct tissues to achieve the adaptive development between the micro- and macrostructure and function. In this article, we divide mechanical stimuli into three types according to forces transmitted by different media in bioreactors, namely forces transmitted through the liquid medium, solid medium, or other media, then we review and summarize the research status of bioreactors for cartilage tissue engineering (CTE), mainly focusing on the effects of diverse mechanical stimuli on engineered cartilage. Based on current researches, there are several motion patterns in knee joints; but compression, tension, shear, fluid shear, or hydrostatic pressure each only partially reflects the mechanical condition in vivo. In this study, we propose that rolling-sliding-compression load consists of various stimuli that will represent better mechanical environment in CTE. In addition, engineers often ignore the importance of biochemical factors to the growth and development of engineered cartilage. In our point of view, only by fully considering synergistic effects of mechanical and biochemical factors can we find appropriate culture conditions for functional cartilage constructs. Once again, rolling-sliding-compression load under appropriate biochemical conditions may be conductive to realize the adaptive development between the structure and function of engineered cartilage in vitro.
[Mh] Términos MeSH primario: Reactores Biológicos
[Mh] Términos MeSH secundario: Cartílago
Cartílago Articular
Condrocitos
Estrés Mecánico
Ingeniería de Tejidos
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Em] Mes de ingreso:1803
[Cu] Fecha actualización por clase:180307
[Lr] Fecha última revisión:180307
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170503
[St] Status:MEDLINE
[do] DOI:10.1089/ten.TEB.2016.0427


  8 / 167320 MEDLINE  
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[PMID]:28453842
[Au] Autor:Lipowski D; Popiel M; Perlejewski K; Nakamura S; Bukowska-Osko I; Rzadkiewicz E; Dzieciatkowski T; Milecka A; Wenski W; Ciszek M; Debska-Slizien A; Ignacak E; Cortes KC; Pawelczyk A; Horban A; Radkowski M; Laskus T
[Ad] Dirección:Department of Infectious Diseases, Warsaw Medical University, Warsaw, Poland.
[Ti] Título:A Cluster of Fatal Tick-borne Encephalitis Virus Infection in Organ Transplant Setting.
[So] Fuente:J Infect Dis;215(6):896-901, 2017 03 15.
[Is] ISSN:1537-6613
[Cp] País de publicación:United States
[La] Idioma:eng
[Ab] Resumen:Background: Tick-borne encephalitis virus (TBEV) infection has become a major health problem in Europe and is currently a common cause of viral brain infection in many countries. Encephalitis in transplant recipients, althrough rare, is becoming a recognized complication. Our study provides the first description of transmission of TBEV through transplantation of solid organs. Methods: Three patients who received solid organ transplants from a single donor (2 received kidney, and 1 received liver) developed encephalitis 17-49 days after transplantation and subsequently died. Blood and autopsy tissue samples were tested by next-generation sequencing (NGS) and reverse transcription polymerase chain reaction (RT-PCR). Results: All 3 recipients were first analyzed in autopsy brain tissue samples and/or cerebrospinal fluid by NGS, which yielded 24-52 million sequences per sample and 9-988 matched TBEV sequences in each patient. The presence of TBEV was confirmed by RT-PCR in all recipients and in the donor, and direct sequencing of amplification products corroborated the presence of the same viral strain. Conclusions: We demonstrated transmission of TBEV by transplantation of solid organs. In such a setting, TBEV infection may be fatal, probably due to pharmacological immunosuppression. Organ donors should be screened for TBEV when coming from or visiting endemic areas.
[Mh] Términos MeSH primario: Encéfalo/virología
Virus de la Encefalitis Transmitidos por Garrapatas/aislamiento & purificación
Encefalitis Transmitida por Garrapatas/transmisión
Trasplante de Órganos/efectos adversos
Donantes de Tejidos
[Mh] Términos MeSH secundario: Adulto
Autopsia
Selección de Donante
Encefalitis Transmitida por Garrapatas/etiología
Resultado Fatal
Seres Humanos
Masculino
Mediana Edad
Polonia
Complicaciones Posoperatorias/etiología
ARN Viral/sangre
Análisis de Secuencia de ARN
[Pt] Tipo de publicación:CASE REPORTS; JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (RNA, Viral)
[Em] Mes de ingreso:1706
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:AIM; IM
[Da] Fecha de ingreso para procesamiento:170429
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix040


  9 / 167320 MEDLINE  
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[PMID]:28452328
[Au] Autor:Golafshan N; Gharibi H; Kharaziha M; Fathi M
[Ad] Dirección:Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
[Ti] Título:A facile one-step strategy for development of a double network fibrous scaffold for nerve tissue engineering.
[So] Fuente:Biofabrication;9(2):025008, 2017 04 28.
[Is] ISSN:1758-5090
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:The aim of this study was to develop a novel double network scaffold composed of polycaprolactone fumarate (PCLF) and eggshell membrane (ESM) (ESM:PCLF) by using the vacuum infiltration method. Compared to ESM, the mechanical properties of double network scaffold were significantly improved, depending on the solvents applied for double network scaffold formation; acetic acid and dichloromethane. Noticeably, the toughness and strength of double network scaffold prepared using acetic acid were significantly improved compared to ESM (26.6 and 25 times, respectively) attributed to the existence of hydrophilic functional groups in acetic acid which made ESM flexible to absorb further PCLF solution. To assess the effect of double network formation on the biological behavior of ESM, the attachment, proliferation and spreading of PC12 cells cultured on the ESM:PCLF scaffolds were evaluated. Results revealed that the number of cells attached on double network ESM:PCLF scaffold were nearly similar to ESM and significantly higher than that of on the tissue culture plate (2.6 times) and PCLF film (1.7 times). It is envisioned that the offered ESM:PCLF double network scaffold might have great potential to develop the constructs for nerve regeneration.
[Mh] Términos MeSH primario: Tejido Nervioso/fisiología
Ingeniería de Tejidos
Andamios del Tejido/química
[Mh] Términos MeSH secundario: Animales
Materiales Biocompatibles/síntesis química
Materiales Biocompatibles/química
Materiales Biocompatibles/farmacología
Membrana Celular/química
Movimiento Celular/efectos de los fármacos
Proliferación Celular/efectos de los fármacos
Supervivencia Celular/efectos de los fármacos
Cáscara de Huevo
Interacciones Hidrofóbicas e Hidrofílicas
Microscopía Electrónica de Rastreo
Regeneración Nerviosa/efectos de los fármacos
Células PC12
Poliésteres/química
Ratas
Espectroscopía Infrarroja por Transformada de Fourier
Rayos Ultravioleta
[Pt] Tipo de publicación:JOURNAL ARTICLE
[Nm] Nombre de substancia:
0 (Biocompatible Materials); 0 (Polyesters); 0 (poly(caprolactone fumarate))
[Em] Mes de ingreso:1802
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:170429
[St] Status:MEDLINE
[do] DOI:10.1088/1758-5090/aa68ed


  10 / 167320 MEDLINE  
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[PMID]:27775923
[Au] Autor:Ortuño-Lizarán I; Vilariño-Feltrer G; Martínez-Ramos C; Pradas MM; Vallés-Lluch A
[Ad] Dirección:Centre for Biomaterials and Tissue Engineering, Universitat Politècnica de València, Cno. de Vera s/n, E-46022, Valencia, Spain. Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Spain.
[Ti] Título:Influence of synthesis parameters on hyaluronic acid hydrogels intended as nerve conduits.
[So] Fuente:Biofabrication;8(4):045011, 2016 10 24.
[Is] ISSN:1758-5090
[Cp] País de publicación:England
[La] Idioma:eng
[Ab] Resumen:Hydrogels have widely been proposed lately as strategies for neural tissue regeneration, but there are still some issues to be solved before their efficient use in tissue engineering of trauma, stroke or the idiopathic degeneration of the nervous system. In a previous work of the authors a novel Schwann-cell structure with the shape of a hollow cylinder was obtained using a three-dimensional conduit based in crosslinked hyaluronic acid as template. This original engineered tissue of tightly joined Schwann cells obtained in a conduit lumen having 400 µm in diameter is a consequence of specific cell-material interactions. In the present work we analyze the influence of the hydrogel concentration and of the drying process on the physicochemical and biological performance of the resulting tubular scaffolds, and prove that the cylinder-like cell sheath obtains also in scaffolds of a larger inner diameter. The diffusion of glucose and of the protein BSA through the scaffolds is studied and characterized, as well as the enzymatic degradation kinetics of the lyophilized conduits. This can be modulated from a couple of weeks to several months by varying the concentration of hyaluronic acid in the starting solution. These findings allow to improve the performance of hyaluronan intended for neural conduits, and open the way to scaffolds with tunable degradation rate adapted to the site and severity of the injury.
[Mh] Términos MeSH primario: Ácido Hialurónico/química
Hidrogeles/química
Andamios del Tejido/química
[Mh] Términos MeSH secundario: Animales
Bovinos
Línea Celular
Proliferación Celular
Supervivencia Celular
Difusión
Glucosa/metabolismo
Hidrogeles/síntesis química
Regeneración Nerviosa
Porosidad
Ratas
Células de Schwann/citología
Células de Schwann/metabolismo
Células de Schwann/patología
Albúmina Sérica Bovina/metabolismo
Ingeniería de Tejidos
[Pt] Tipo de publicación:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nombre de substancia:
0 (Hydrogels); 27432CM55Q (Serum Albumin, Bovine); 9004-61-9 (Hyaluronic Acid); IY9XDZ35W2 (Glucose)
[Em] Mes de ingreso:1711
[Cu] Fecha actualización por clase:180308
[Lr] Fecha última revisión:180308
[Sb] Subgrupo de revista:IM
[Da] Fecha de ingreso para procesamiento:161025
[St] Status:MEDLINE



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