Database : MEDLINE
Search on : African and Swine and Fever [Words]
References found : 1417 [refine]
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[PMID]: 29519515
[Au] Autor:Franzoni G; Graham SP; Sanna G; Angioi P; Fiori MS; Anfossi A; Amadori M; Dei Giudici S; Oggiano A
[Ad] Address:Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, 07100, Italy. Electronic address: giulia.franzoni@izs-sardegna.it.
[Ti] Title:Interaction of porcine monocyte-derived dendritic cells with African swine fever viruses of diverse virulence.
[So] Source:Vet Microbiol;216:190-197, 2018 Mar.
[Is] ISSN:1873-2542
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:African swine fever (ASF) is a devastating disease for which there is no vaccine. The ASF virus (ASFV) can infect dendritic cell (DC), but despite the critical role these cells play in induction of adaptive immunity, few studies investigated their response to ASFV infection. We characterized the in vitro interactions of porcine monocyte-derived DCs (moDC) with a virulent (22653/14), a low virulent (NH/P68) and an avirulent (BA71V) ASFV strain. At a high multiplicity of infection (MOI = 1), all three strains infected immature moDC. Maturation of moDC, with IFN-α/TNF-α, increased susceptibility to infection with 22653/14 and other virulent strains, but reduced susceptibility to NH/P68 and BA71V. The reduced moDC susceptibility to BA71V/NH/P68 was IFN-α mediated, whereas increased susceptibility to 22653/14 was induced by TNF-α. Using an MOI of 0.01, we observed that BA71V replicated less efficiently in moDC compared to the other isolates and we detected increased replication of NH/P68 compared to 22653/14. We observed that BA71V and NH/P68, but not 22653/14, downregulated expression of MHC class I on infected cells. All three strains decreased CD16 expression on moDC, whereas ASFV infection resulted in CD80/86 down-regulation and MHC class II DR up-regulation on mature moDC. None of the tested strains induced a strong cytokine response to ASFV and only modest IL-1α was released after BA71V infection. Overall our results revealed differences between strains and suggest that ASFV has evolved mechanisms to replicate covertly in inflammatory DC, which likely impairs the induction of an effective immune response.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:In-Process

  2 / 1417 MEDLINE  
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[PMID]: 29519508
[Au] Autor:Portugal R; Leitão A; Martins C
[Ad] Address:Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Avenida da Universidade Técnica, 1300-477 Lisboa, Portugal. Electronic address: raquel.portugal@pirbright.ac.uk.
[Ti] Title:Modulation of type I interferon signaling by African swine fever virus (ASFV) of different virulence L60 and NHV in macrophage host cells.
[So] Source:Vet Microbiol;216:132-141, 2018 Mar.
[Is] ISSN:1873-2542
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:ASFV causes an important disease of domestic swine and wild boar. Currently no vaccine is available, highlighting the necessity to understand ASFV modulation of innate immune responses in natural host cells. With this aim, macrophage cultures enriched in SWC9 and CD163 differentiation markers were infected in parallel with high virulent ASFV/L60 and low virulent ASFV/NHV, the latter lacking MGF 360 and 505/530 genes associated with type I interferon (IFN I) control. IFN I production and signaling were studied after completion of the viral cycles. None of the viruses increased IFN I production in host cells, and accordingly, didn't cause activation of the central mediator of the pathway IRF3. However, upon stimulation by poly:IC treatment during infections, L60 and NHV similarly inhibited IFN I production. This didn't seem to depend on IRF3 modulation since its activation levels were not significantly decreased in L60 infection and were even increased in NHV's, in comparison to stimulated mock infections. The infections didn't evidently activate JAK-STAT pathway mediators STAT1 and STAT2, but did increase expression of interferon stimulated genes (ISGs), to higher levels in NHV than L60 infection. Interestingly, in presence of IFN-α, L60 but not NHV was able to decrease significantly the expression of some of the ISGs tested. Overall, both L60 and NHV were able to inhibit IFN I production in macrophages, through a mechanism not dependent on IRF3 modulation. The high virulent isolate showed however a more effective control of the downstream ISGs expression pathway.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:In-Process

  3 / 1417 MEDLINE  
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[PMID]: 29327614
[Au] Autor:Weber S; Hakobyan A; Zakaryan H; Doerfler W
[Ad] Address:Institute for Clinical & Molecular Virology, Friedrich-Alexander University Erlangen-Nürnberg, 91054 Erlangen, Germany.
[Ti] Title:Intracellular African swine fever virus DNA remains unmethylated in infected Vero cells.
[So] Source:Epigenomics;10(3):289-299, 2018 Mar.
[Is] ISSN:1750-192X
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:AIM: Sequence-specific CpG methylation of eukaryotic promoters is an important epigenetic signal for long-term gene silencing. We have now studied the methylation status of African swine fever virus (ASFV) DNA at various times after infection of Vero cells in culture. METHODS & RESULTS: ASFV DNA was detectable throughout the infection cycle and was found unmethylated in productively infected Vero cells as documented by bisulfite sequencing of 13 viral DNA segments. CONCLUSION: ASFV DNA does not become de novo methylated in the course of infection in selected segments spread across the entire genome. Thus DNA methylation does not interfere with ASFV genome transcription. Lack of de novo methylation has previously been observed for free intracellular viral DNA in cells permissively infected with human adenoviruses, with human papillomaviruses and others.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[St] Status:In-Data-Review
[do] DOI:10.2217/epi-2017-0131

  4 / 1417 MEDLINE  
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[PMID]: 29246986
[Au] Autor:Gibbens N
[Ti] Title:African swine fever and the risks of feeding food waste to pigs.
[So] Source:Vet Rec;181(24):642, 2017 12 16.
[Is] ISSN:2042-7670
[Cp] Country of publication:England
[La] Language:eng
[Mh] MeSH terms primary: African Swine Fever/epidemiology
Animal Feed/adverse effects
Waste Products/adverse effects
[Mh] MeSH terms secundary: Animals
Risk
Swine
United Kingdom/epidemiology
[Pt] Publication type:NEWS
[Nm] Name of substance:0 (Waste Products)
[Em] Entry month:1803
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[Js] Journal subset:IM
[Da] Date of entry for processing:171217
[St] Status:MEDLINE

  5 / 1417 MEDLINE  
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[PMID]: 29496103
[Au] Autor:Ouma E; Dione M; Birungi R; Lule P; Mayega L; Dizyee K
[Ad] Address:International Livestock Research Institute, c/o Bioversity International P.O. Box 24384 Kampala, Uganda. Electronic address: E.A.Ouma@cgiar.org.
[Ti] Title:African swine fever control and market integration in Ugandan peri-urban smallholder pig value chains: An ex-ante impact assessment of interventions and their interaction.
[So] Source:Prev Vet Med;151:29-39, 2018 Mar 01.
[Is] ISSN:1873-1716
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Pig production in peri-urban smallholder value chains in Uganda is severely constrained by impact of disease, particularly African swine fever (ASF), and the economic consequences of an inefficient pig value chain. Interventions in the form of biosecurity to control ASF disease outbreaks and pig business hub models to better link smallholder farmers to pig markets have the potential to address the constraints. However, there is a dearth of evidence of the effects of the interventions on performance and distribution of outcomes along the pig value chain. An ex-ante impact assessment utilising System Dynamics model was used to assess the impact of the interventions in peri-urban pig value chains in Masaka district. The results showed that although implementation of biosecurity interventions results in reduction of ASF outbreaks, it also leads to a 6.3% reduction in farmer profit margins per year but more than 7% increase in other value chain actors' margins. The pig business hub intervention alone results in positive margins for all value chain actors but minimal reduction in ASF outbreaks. When biosecurity and the pig business hub interventions are implemented together, the interaction effects of the interventions result in positive outcomes for both the control of ASF and improvement in farmers' margins. Farmers may therefore be unwilling to adopt biosecurity practices if implemented alone to control ASF outbreaks unless there is a corresponding financial incentive to compensate for the high costs. This has implications for policy or developing institutions to facilitate cost sharing arrangement among chain actors and/or third party subsidy to provide incentives for producers to adopt biosecurity measures.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180302
[Lr] Last revision date:180302
[St] Status:In-Process

  6 / 1417 MEDLINE  
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[PMID]: 29194985
[Au] Autor:Erickson A; Fisher M; Furukawa-Stoffer T; Ambagala A; Hodko D; Pasick J; King DP; Nfon C; Ortega Polo R; Lung O
[Ad] Address:Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food Inspection Agency, Lethbridge, AB, Canada.
[Ti] Title:A multiplex reverse transcription PCR and automated electronic microarray assay for detection and differentiation of seven viruses affecting swine.
[So] Source:Transbound Emerg Dis;65(2):e272-e283, 2018 Apr.
[Is] ISSN:1865-1682
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Microarray technology can be useful for pathogen detection as it allows simultaneous interrogation of the presence or absence of a large number of genetic signatures. However, most microarray assays are labour-intensive and time-consuming to perform. This study describes the development and initial evaluation of a multiplex reverse transcription (RT)-PCR and novel accompanying automated electronic microarray assay for simultaneous detection and differentiation of seven important viruses that affect swine (foot-and-mouth disease virus [FMDV], swine vesicular disease virus [SVDV], vesicular exanthema of swine virus [VESV], African swine fever virus [ASFV], classical swine fever virus [CSFV], porcine respiratory and reproductive syndrome virus [PRRSV] and porcine circovirus type 2 [PCV2]). The novel electronic microarray assay utilizes a single, user-friendly instrument that integrates and automates capture probe printing, hybridization, washing and reporting on a disposable electronic microarray cartridge with 400 features. This assay accurately detected and identified a total of 68 isolates of the seven targeted virus species including 23 samples of FMDV, representing all seven serotypes, and 10 CSFV strains, representing all three genotypes. The assay successfully detected viruses in clinical samples from the field, experimentally infected animals (as early as 1 day post-infection (dpi) for FMDV and SVDV, 4 dpi for ASFV, 5 dpi for CSFV), as well as in biological material that were spiked with target viruses. The limit of detection was 10 copies/µl for ASFV, PCV2 and PRRSV, 100 copies/µl for SVDV, CSFV, VESV and 1,000 copies/µl for FMDV. The electronic microarray component had reduced analytical sensitivity for several of the target viruses when compared with the multiplex RT-PCR. The integration of capture probe printing allows custom onsite array printing as needed, while electrophoretically driven hybridization generates results faster than conventional microarrays that rely on passive hybridization. With further refinement, this novel, rapid, highly automated microarray technology has potential applications in multipathogen surveillance of livestock diseases.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180302
[Lr] Last revision date:180302
[St] Status:In-Process
[do] DOI:10.1111/tbed.12749

  7 / 1417 MEDLINE  
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[PMID]: 29489860
[Au] Autor:Alkhamis MA; Gallardo C; Jurado C; Soler A; Arias M; Sánchez-Vizcaíno JM
[Ad] Address:Faculty of Public Heath, Health Sciences Centre, Kuwait University, Kuwait City, Kuwait.
[Ti] Title:Phylodynamics and evolutionary epidemiology of African swine fever p72-CVR genes in Eurasia and Africa.
[So] Source:PLoS One;13(2):e0192565, 2018.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:African swine fever (ASF) is a complex infectious disease of swine that constitutes devastating impacts on animal health and the world economy. Here, we investigated the evolutionary epidemiology of ASF virus (ASFV) in Eurasia and Africa using the concatenated gene sequences of the viral protein 72 and the central variable region of isolates collected between 1960 and 2015. We used Bayesian phylodynamic models to reconstruct the evolutionary history of the virus, to identify virus population demographics and to quantify dispersal patterns between host species. Results suggest that ASFV exhibited a significantly high evolutionary rate and population growth through time since its divergence in the 18th century from East Africa, with no signs of decline till recent years. This increase corresponds to the growing pig trade activities between continents during the 19th century, and may be attributed to an evolutionary drift that resulted from either continuous circulation or maintenance of the virus within Africa and Eurasia. Furthermore, results implicate wild suids as the ancestral host species (root state posterior probability = 0.87) for ASFV in the early 1700s in Africa. Moreover, results indicate the transmission cycle between wild suids and pigs is an important cycle for ASFV spread and maintenance in pig populations, while ticks are an important natural reservoir that can facilitate ASFV spread and maintenance in wild swine populations. We illustrated the prospects of phylodynamic methods in improving risk-based surveillance, support of effective animal health policies, and epidemic preparedness in countries at high risk of ASFV incursion.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180228
[Lr] Last revision date:180228
[St] Status:In-Data-Review
[do] DOI:10.1371/journal.pone.0192565

  8 / 1417 MEDLINE  
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[PMID]: 29486878
[Au] Autor:Sánchez-Cordón PJ; Montoya M; Reis AL; Dixon LK
[Ad] Address:The Pirbright Institute, Pirbright, Woking, Surrey GU24 0NF, UK.
[Ti] Title:African swine fever: A re-emerging viral disease threatening the global pig industry.
[So] Source:Vet J;233:41-48, 2018 Mar.
[Is] ISSN:1532-2971
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:African swine fever (ASF) recently has spread beyond sub-Saharan Africa to the Trans-Caucasus region, parts of the Russian Federation and Eastern Europe. In this new epidemiological scenario, the disease has similarities, but also important differences, compared to the situation in Africa, including the substantial involvement of wild boar. A better understanding of this new situation will enable better control and prevent further spread of disease. In this article, these different scenarios are compared, and recent information on the pathogenesis of ASF virus strains, the immune response to infection and prospects for developing vaccines is presented. Knowledge gaps and the prospects for future control are discussed.
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Em] Entry month:1803
[Cu] Class update date: 180228
[Lr] Last revision date:180228
[St] Status:In-Data-Review

  9 / 1417 MEDLINE  
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[PMID]: 29472632
[Au] Autor:Freitas FB; Frouco G; Martins C; Ferreira F
[Ad] Address:Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, 1300-477, Portugal.
[Ti] Title:African swine fever virus encodes for an E2-ubiquitin conjugating enzyme that is mono- and di-ubiquitinated and required for viral replication cycle.
[So] Source:Sci Rep;8(1):3471, 2018 Feb 22.
[Is] ISSN:2045-2322
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:African swine fever virus is the etiological agent of a contagious and fatal acute haemorrhagic viral disease for which there are no vaccines or therapeutic options. The ASFV encodes for a putative E2 ubiquitin conjugating enzyme (ORF I215L) that shows sequence homology with eukaryotic counterparts. In the present study, we showed that pI215L acts as an E2-ubiquitin like enzyme in a large range of pH values and temperatures, after short incubation times. Further experiments revealed that pI215L is polyubiquitinated instead of multi-mono-ubiquitinated and Cys85 residue plays an essential role in the transthioesterification reaction. In infected cells, I215L gene is transcribed from 2 hours post infection and immunoblot analysis confirmed that pI215L is expressed from 4 hpi. Immunofluorescence studies revealed that pI215L is recruited to viral factories from 8 hpi and a diffuse distribution pattern throughout the nucleus and cytoplasm. siRNA studies suggested that pI215L plays a critical role in the transcription of late viral genes and viral DNA replication. Altogether, our results emphasize the potential use of this enzyme as target for drug and vaccine development against ASF.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180227
[Lr] Last revision date:180227
[St] Status:In-Data-Review
[do] DOI:10.1038/s41598-018-21872-2

  10 / 1417 MEDLINE  
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[PMID]: 29453406
[Au] Autor:Borca MV; Holinka LG; Berggren KA; Gladue DP
[Ad] Address:Agricultural Research Service (ARS), Plum Island Animal Disease Center, Greenport, NY, 11944, USA.
[Ti] Title:CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses.
[So] Source:Sci Rep;8(1):3154, 2018 Feb 16.
[Is] ISSN:2045-2322
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:African swine fever virus (ASFV) causes a highly contagious disease called African swine fever. This disease is often lethal for domestic pigs, causing extensive losses for the swine industry. ASFV is a large and complex double stranded DNA virus. Currently there is no commercially available treatment or vaccine to prevent this devastating disease. Development of recombinant ASFV for producing live-attenuated vaccines or studying the involvement of specific genes in virus virulence has relied on the relatively rare event of homologous recombination in primary swine macrophages, causing difficulty to purify the recombinant virus from the wild-type parental ASFV. Here we present the use of the CRISPR-Cas9 gene editing system as a more robust and efficient system to produce recombinant ASFVs. Using CRISPR-Cas9 a recombinant virus was efficiently developed by deleting the non-essential gene 8-DR from the genome of the highly virulent field strain Georgia07 using swine macrophages as cell substrate.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180222
[Lr] Last revision date:180222
[St] Status:In-Data-Review
[do] DOI:10.1038/s41598-018-21575-8


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