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  1 / 27 MEDLINE  
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[PMID]: 28492177
[Au] Autor:López JR; Lorenzo L; Alcantara R; Navas JI
[Ad] Address:IFAPA Centro Agua del Pino, Junta de Andalucía, Carretera El Rompido-Punta Umbría km 3.8, CP21450 Cartaya, Huelva, Spain.
[Ti] Title:Characterization of Aliivibrio fischeri strains associated with disease outbreak in brill Scophthalmus rhombus.
[So] Source:Dis Aquat Organ;124(3):215-222, 2017 05 11.
[Is] ISSN:0177-5103
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Three bacterial isolates were recovered from a disease outbreak with high mortality affecting brill Scophthalmus rhombus (Linnaeus, 1758). Moribund fish showed no external signs of disease, but plentiful haemorrhages were observed in liver. On the basis of phenotypic and genotypic characterization, the isolates were identified as Aliivibrio fischeri. The phenotypic profile of the isolates was basically similar to that of the type strain of this species, although some discrepancies were observed, mainly in the BIOLOG GN profile. The main cellular fatty acids of strain a591 were also consistent with this species. The highest 16S rDNA sequence similarities were recorded with the type strain of A. fischeri (99.07%); other Aliivibrio species showed similarity values below 96%. The highest sequence similarities with gyrB, rpoD and recA genes were also recorded with A. fischeri type strain (99.31, 98.99 and 95.29% similarity, respectively). DNA-DNA hybridization assays confirmed that these isolates belong to A. fischeri; levels of DNA relatedness were 73.5 to 86.2% with isolate a591 (reciprocal values of 86.9 to 99.04%). Finally, a virulence evaluation of the isolates using Senegalese sole fry was also performed; significant mortalities (100% mortality within 5 d) were recorded by intraperitoneal injection, but only with high doses of bacteria (2 × 106 cfu g-1 body weight).
[Mh] MeSH terms primary: Aliivibrio fischeri/genetics
Disease Outbreaks/veterinary
Fish Diseases/microbiology
Flatfishes/microbiology
Gram-Negative Bacterial Infections/veterinary
[Mh] MeSH terms secundary: Animals
Aquaculture
DNA, Bacterial/genetics
Gram-Negative Bacterial Infections/microbiology
Phylogeny
Polymerase Chain Reaction
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (DNA, Bacterial)
[Em] Entry month:1708
[Cu] Class update date: 170803
[Lr] Last revision date:170803
[Js] Journal subset:IM
[Da] Date of entry for processing:170512
[St] Status:MEDLINE
[do] DOI:10.3354/dao03123

  2 / 27 MEDLINE  
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[PMID]: 27376368
[Au] Autor:Caianelo M; Rodrigues-Silva C; Maniero MG; Guimarães JR
[Ad] Address:School of Civil Engineering, Architecture and Urban Design, University of Campinas, P.O. Box 6143, 13083-889, Campinas, Brazil.
[Ti] Title:Antimicrobial activity against Gram-positive and Gram-negative bacteria during gatifloxacin degradation by hydroxyl radicals.
[So] Source:Environ Sci Pollut Res Int;24(7):6288-6298, 2017 Mar.
[Is] ISSN:1614-7499
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Gatifloxacin, an antimicrobial drug belonging to the fluoroquinolone family, is active against Gram-positive and Gram-negative bacteria and is extensively used for the control of infections in humans. The presence of the drug in environmental matrices has already been reported. This study investigated the degradation of gatifloxacin in water by hydroxyl radicals generated by the UV /H O process ([Formula: see text] 0.4-2.4 mmol L ) and evaluated the capacity of the radicals to reduce the antimicrobial activity against Gram-positive and Gram-negative bacteria. Acute toxicity assays were performed with Vibrio fischeri, and the degradation products were proposed. The hydroxyl radicals formed in the processes were able to degrade the fluoroquinolone and remove the antimicrobial activity from the aqueous solution. Approximately 97 % gatifloxacin degradation was observed after applying 2.4 mmol L of initial H O concentration and 20 min of UVC irradiation (130 J s ). The acute toxicity assays showed that the toxicity of the treated solution for V. fischeri increased as the gatifloxacin concentration in the solution decreased.
[Mh] MeSH terms primary: Anti-Bacterial Agents/chemistry
Anti-Bacterial Agents/pharmacology
Fluoroquinolones/chemistry
Fluoroquinolones/pharmacology
Gram-Negative Bacteria/drug effects
Gram-Positive Bacteria/drug effects
Hydroxyl Radical/chemistry
[Mh] MeSH terms secundary: Aliivibrio fischeri/drug effects
Anti-Bacterial Agents/toxicity
Fluoroquinolones/toxicity
Humans
Hydrogen Peroxide/chemistry
Microbial Sensitivity Tests
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Anti-Bacterial Agents); 0 (Fluoroquinolones); 3352-57-6 (Hydroxyl Radical); BBX060AN9V (Hydrogen Peroxide); L4618BD7KJ (gatifloxacin)
[Em] Entry month:1705
[Cu] Class update date: 171104
[Lr] Last revision date:171104
[Js] Journal subset:IM
[Da] Date of entry for processing:160705
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-6972-y

  3 / 27 MEDLINE  
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[PMID]: 27072873
[Au] Autor:Kashulin A; Seredkina N; Sørum H
[Ad] Address:Department of Food Safety and Infection Biology, Norwegian University of Life Sciences, Oslo, Norway.
[Ti] Title:Cold-water vibriosis. The current status of knowledge.
[So] Source:J Fish Dis;40(1):119-126, 2017 Jan.
[Is] ISSN:1365-2761
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:The current review for the first time summarizes the findings of the 30 years of research on cold-water vibriosis (CWV). The diseased caused by Aliivibrio salmonicida (earlier known as Vibrio salmonicida) was for the first time described in 1986 and became one of the most important bacterial diseases in salmon aquaculture. The lack of appropriate vaccine hampered development of Atlantic salmon aquaculture until the late 1980s when a novel vaccine allowed dramatic increase in the Atlantic salmon farming. In December 2007, the genus Vibrio was split into two genera and several bacterial species including V. salmonicida were transferred to genus Aliivibrio. The change of the names create significant difficulties with the designation of the CWV disease agent since its abbreviation A. salmonicida became similar to another well-known salmon pathogen Aeromonas salmonicida (A. salmonicida). The disease was considered as controlled by vaccination, but reappeared at Atlantic salmon farms in 2011, this time affecting vaccinated Atlantic salmon. The current review summarizes the knowledge on pathogenesis, vaccination and treatment of CWV and proposes further directions for studying the disease.
[Mh] MeSH terms primary: Fish Diseases/microbiology
Fish Diseases/prevention & control
Vaccination/veterinary
Vibrio Infections/veterinary
Vibrio/physiology
[Mh] MeSH terms secundary: Animals
Aquaculture
Vibrio Infections/microbiology
Vibrio Infections/prevention & control
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Em] Entry month:1705
[Cu] Class update date: 170503
[Lr] Last revision date:170503
[Js] Journal subset:IM
[Da] Date of entry for processing:160414
[St] Status:MEDLINE
[do] DOI:10.1111/jfd.12465

  4 / 27 MEDLINE  
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[PMID]: 26542048
[Au] Autor:Boyd EF; Carpenter MR; Chowdhury N; Cohen AL; Haines-Menges BL; Kalburge SS; Kingston JJ; Lubin JB; Ongagna-Yhombi SY; Whitaker WB
[Ti] Title:Post-Genomic Analysis of Members of the Family Vibrionaceae.
[So] Source:Microbiol Spectr;3(5), 2015 Oct.
[Is] ISSN:2165-0497
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Similar to other genera and species of bacteria, whole genomic sequencing has revolutionized how we think about and address questions of basic Vibrio biology. In this review we examined 36 completely sequenced and annotated members of the Vibrionaceae family, encompassing 12 different species of the genera Vibrio, Aliivibrio, and Photobacterium. We reconstructed the phylogenetic relationships among representatives of this group of bacteria by using three housekeeping genes and 16S rRNA sequences. With an evolutionary framework in place, we describe the occurrence and distribution of primary and alternative sigma factors, global regulators present in all bacteria. Among Vibrio we show that the number and function of many of these sigma factors differs from species to species. We also describe the role of the Vibrio-specific regulator ToxRS in fitness and survival. Examination of the biochemical capabilities was and still is the foundation of classifying and identifying new Vibrio species. Using comparative genomics, we examine the distribution of carbon utilization patterns among Vibrio species as a possible marker for understanding bacteria-host interactions. Finally, we discuss the significant role that horizontal gene transfer, specifically, the distribution and structure of integrons, has played in Vibrio evolution.
[Mh] MeSH terms primary: Aliivibrio/classification
Genetic Variation
Genome, Bacterial
Photobacterium/classification
Phylogeny
Vibrio/classification
[Mh] MeSH terms secundary: Aliivibrio/genetics
Animals
Bacterial Typing Techniques
DNA, Bacterial/chemistry
DNA, Bacterial/genetics
DNA, Ribosomal/chemistry
DNA, Ribosomal/genetics
Evolution, Molecular
Gene Transfer, Horizontal
Genes, Essential
Genes, Regulator
Gram-Negative Bacterial Infections/microbiology
Gram-Negative Bacterial Infections/veterinary
Host-Pathogen Interactions
Humans
Photobacterium/genetics
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Sigma Factor/genetics
Vibrio/genetics
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.; REVIEW
[Nm] Name of substance:0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S); 0 (Sigma Factor)
[Em] Entry month:1608
[Cu] Class update date: 151106
[Lr] Last revision date:151106
[Js] Journal subset:IM
[Da] Date of entry for processing:151107
[St] Status:MEDLINE
[do] DOI:10.1128/microbiolspec.VE-0009-2014

  5 / 27 MEDLINE  
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[PMID]: 25979894
[Au] Autor:Chu T; Huang Y; Hou M; Wang Q; Xiao J; Liu Q; Zhang Y
[Ad] Address:State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.
[Ti] Title:In Vivo Programmed Gene Expression Based on Artificial Quorum Networks.
[So] Source:Appl Environ Microbiol;81(15):4984-92, 2015 Aug.
[Is] ISSN:1098-5336
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The quorum sensing (QS) system, as a well-functioning population-dependent gene switch, has been widely applied in many gene circuits in synthetic biology. In our work, an efficient cell density-controlled expression system (QS) was established via engineering of the Vibrio fischeri luxI-luxR quorum sensing system. In order to achieve in vivo programmed gene expression, a synthetic binary regulation circuit (araQS) was constructed by assembling multiple genetic components, including the quorum quenching protein AiiA and the arabinose promoter ParaBAD, into the QS system. In vitro expression assays verified that the araQS system was initiated only in the absence of arabinose in the medium at a high cell density. In vivo expression assays confirmed that the araQS system presented an in vivo-triggered and cell density-dependent expression pattern. Furthermore, the araQS system was demonstrated to function well in different bacteria, indicating a wide range of bacterial hosts for use. To explore its potential applications in vivo, the araQS system was used to control the production of a heterologous protective antigen in an attenuated Edwardsiella tarda strain, which successfully evoked efficient immune protection in a fish model. This work suggested that the araQS system could program bacterial expression in vivo and might have potential uses, including, but not limited to, bacterial vector vaccines.
[Mh] MeSH terms primary: Aliivibrio fischeri/drug effects
Aliivibrio fischeri/physiology
Arabinose/metabolism
Gene Expression Regulation, Bacterial/drug effects
Quorum Sensing
[Mh] MeSH terms secundary: Aliivibrio fischeri/genetics
Animals
Antigens, Bacterial/biosynthesis
Antigens, Bacterial/genetics
Antigens, Bacterial/immunology
Bacterial Infections/immunology
Bacterial Infections/prevention & control
Bacterial Infections/veterinary
Culture Media/chemistry
Edwardsiella tarda/genetics
Edwardsiella tarda/immunology
Edwardsiella tarda/metabolism
Fish Diseases/immunology
Fish Diseases/prevention & control
Fishes
Gene Expression Profiling
Models, Theoretical
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Antigens, Bacterial); 0 (Culture Media); B40ROO395Z (Arabinose)
[Em] Entry month:1603
[Cu] Class update date: 170220
[Lr] Last revision date:170220
[Js] Journal subset:IM
[Da] Date of entry for processing:150517
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01113-15

  6 / 27 MEDLINE  
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[PMID]: 25672848
[Au] Autor:Gutiérrez-Barranquero JA; Reen FJ; McCarthy RR; O'Gara F
[Ad] Address:BIOMERIT Research Centre, School of Microbiology, University College Cork, National University of Ireland, Cork, Ireland.
[Ti] Title:Deciphering the role of coumarin as a novel quorum sensing inhibitor suppressing virulence phenotypes in bacterial pathogens.
[So] Source:Appl Microbiol Biotechnol;99(7):3303-16, 2015 Apr.
[Is] ISSN:1432-0614
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:The rapid unchecked rise in antibiotic resistance over the last few decades has led to an increased focus on the need for alternative therapeutic strategies for the treatment and clinical management of microbial infections. In particular, small molecules that can suppress microbial virulence systems independent of any impact on growth are receiving increased attention. Quorum sensing (QS) is a cell-to-cell signalling communication system that controls the virulence behaviour of a broad spectrum of bacterial pathogens. QS systems have been proposed as an effective target, particularly as they control biofilm formation in pathogens, a key driver of antibiotic ineffectiveness. In this study, we identified coumarin, a natural plant phenolic compound, as a novel QS inhibitor, with potent anti-virulence activity in a broad spectrum of pathogens. Using a range of biosensor systems, coumarin was active against short, medium and long chain N-acyl-homoserine lactones, independent of any effect on growth. To determine if this suppression was linked to anti-virulence activity, key virulence systems were studied in the nosocomial pathogen Pseudomonas aeruginosa. Consistent with suppression of QS, coumarin inhibited biofilm, the production of phenazines and swarming motility in this organism potentially linked to reduced expression of the rhlI and pqsA quorum sensing genes. Furthermore, coumarin significantly inhibited biofilm formation and protease activity in other bacterial pathogens and inhibited bioluminescence in Aliivibrio fischeri. In light of these findings, coumarin would appear to have potential as a novel quorum sensing inhibitor with a broad spectrum of action.
[Mh] MeSH terms primary: Coumarins/pharmacology
Gram-Negative Bacteria/drug effects
Gram-Positive Bacteria/drug effects
Quorum Sensing/drug effects
[Mh] MeSH terms secundary: Acyl-Butyrolactones
Anti-Bacterial Agents/pharmacology
Biofilms/drug effects
Gram-Negative Bacteria/pathogenicity
Gram-Positive Bacteria/pathogenicity
Gram-Positive Bacteria/physiology
Phenotype
Pseudomonas aeruginosa/drug effects
Pseudomonas aeruginosa/pathogenicity
Pseudomonas aeruginosa/physiology
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Acyl-Butyrolactones); 0 (Anti-Bacterial Agents); 0 (Coumarins); A4VZ22K1WT (coumarin)
[Em] Entry month:1605
[Cu] Class update date: 150319
[Lr] Last revision date:150319
[Js] Journal subset:IM
[Da] Date of entry for processing:150213
[St] Status:MEDLINE
[do] DOI:10.1007/s00253-015-6436-1

  7 / 27 MEDLINE  
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[PMID]: 25586643
[Au] Autor:Norsworthy AN; Visick KL
[Ad] Address:Department of Microbiology and Immunology, Loyola University Medical Center, 2160 S. First Ave., Maywood, IL, 60153, USA.
[Ti] Title:Signaling between two interacting sensor kinases promotes biofilms and colonization by a bacterial symbiont.
[So] Source:Mol Microbiol;96(2):233-48, 2015 Apr.
[Is] ISSN:1365-2958
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Cells acclimate to fluctuating environments by utilizing sensory circuits. One common sensory pathway used by bacteria is two-component signaling (TCS), composed of an environmental sensor [the sensor kinase (SK)] and a cognate, intracellular effector [the response regulator (RR)]. The squid symbiont Vibrio fischeri uses an elaborate TCS phosphorelay containing a hybrid SK, RscS, and two RRs, SypE and SypG, to control biofilm formation and host colonization. Here, we found that another hybrid SK, SypF, was essential for biofilms by functioning downstream of RscS to directly control SypE and SypG. Surprisingly, although wild-type SypF functioned as an SK in vitro, this activity was dispensable for colonization. In fact, only a single non-enzymatic domain within SypF, the HPt domain, was critical in vivo. Remarkably, this domain within SypF interacted with RscS to permit a bypass of RscS's own HPt domain and SypF's enzymatic function. This represents the first in vivo example of a functional SK that exploits the enzymatic activity of another SK, an adaptation that demonstrates the elegant plasticity in the arrangement of TCS regulators.
[Mh] MeSH terms primary: Aliivibrio Infections/veterinary
Aliivibrio fischeri/enzymology
Aliivibrio fischeri/growth & development
Bacterial Proteins/metabolism
Biofilms
Decapodiformes/microbiology
Protein Kinases/metabolism
[Mh] MeSH terms secundary: Aliivibrio Infections/microbiology
Aliivibrio fischeri/genetics
Aliivibrio fischeri/physiology
Animals
Bacterial Proteins/genetics
Gene Expression Regulation, Bacterial
Protein Kinases/genetics
Signal Transduction
Symbiosis
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Bacterial Proteins); EC 2.7.- (Protein Kinases)
[Em] Entry month:1601
[Cu] Class update date: 170220
[Lr] Last revision date:170220
[Js] Journal subset:IM
[Da] Date of entry for processing:150115
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.12932

  8 / 27 MEDLINE  
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[PMID]: 25189803
[Au] Autor:Wagil M; Bialk-Bielinska A; Puckowski A; Wychodnik K; Maszkowska J; Mulkiewicz E; Kumirska J; Stepnowski P; Stolte S
[Ad] Address:Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, ul. Wita Stwosza 63, 80-308, Gdansk, Poland.
[Ti] Title:Toxicity of anthelmintic drugs (fenbendazole and flubendazole) to aquatic organisms.
[So] Source:Environ Sci Pollut Res Int;22(4):2566-73, 2015 Feb.
[Is] ISSN:1614-7499
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Flubendazole (FLU) and fenbendazole (FEN) belong to benzimidazoles-pharmaceuticals widely used in veterinary and human medicine for the treatment of intestinal parasites as well as for the treatment of systemic worm infections. In recent years, usage of these drugs increased, which resulted in a larger contamination of the environment and possible negative effects on biota. Hence, in our research, we investigated an aquatic ecotoxicity of these pharmaceuticals towards: marine bacteria (Vibrio fischeri), green algae (Scenedesmus vacuolatus), duckweed (Lemna minor) and crustacean (Daphnia magna). Ecotoxicity tests were combined with chemical analysis in order to investigate the actual exposure concentration of the compounds used in the experiment as well as to stability and adsorption studies. As a result, study evaluating sensitivity of different aquatic organisms to these compounds and new ecotoxicological data is presented. The strongest negative impact of FLU and FEN was observed to D. magna.
[Mh] MeSH terms primary: Anthelmintics/toxicity
Fenbendazole/toxicity
Mebendazole/analogs & derivatives
Water Pollutants, Chemical/toxicity
[Mh] MeSH terms secundary: Aliivibrio fischeri/drug effects
Aliivibrio fischeri/metabolism
Animals
Araceae/drug effects
Araceae/growth & development
Daphnia/drug effects
Daphnia/growth & development
Mebendazole/toxicity
Scenedesmus/drug effects
Scenedesmus/physiology
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Anthelmintics); 0 (Water Pollutants, Chemical); 621BVT9M36 (Fenbendazole); 81G6I5V05I (Mebendazole); R8M46911LR (flubendazole)
[Em] Entry month:1506
[Cu] Class update date: 150804
[Lr] Last revision date:150804
[Js] Journal subset:IM
[Da] Date of entry for processing:140906
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-014-3497-0

  9 / 27 MEDLINE  
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[PMID]: 25027277
[Au] Autor:Hatje E; Neuman C; Stevenson H; Bowman JP; Katouli M
[Ad] Address:Genecology Research Centre, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore DC, QLD, 4558, Australia.
[Ti] Title:Population dynamics of Vibrio and Pseudomonas species isolated from farmed Tasmanian Atlantic salmon (Salmo salar L.): a seasonal study.
[So] Source:Microb Ecol;68(4):679-87, 2014 Nov.
[Is] ISSN:1432-184X
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Vibrio and Pseudomonas species have been shown to be part of the normal microbiota of Atlantic salmon (Salmo salar L.), with some strains causing disease in fish. The factors affecting their prevalence and persistence in the salmon gut, however, have not been well studied. In this study, we collected 340 Vibrio and 150 Pseudomonas isolates from the hindgut of farmed Tasmanian Atlantic salmon, fed with two commercially available diets. Samples were collected every 6-8 weeks between July 2011 and May 2012. Isolates from selective agar were initially identified using biochemical tests and confirmed using genus-specific primers and 16S ribosomal RNA (16S rRNA) sequencing. Random amplified polymorphic DNA (RAPD) PCR was used to type both Pseudomonas and Vibrio; the latter was further typed using a biochemical fingerprinting method (PhP-RV plates). We observed low species diversity with strains comprising Vibrio ichthyoenteri/Vibrio scophthalmi, Vibrio crassostreae/Vibrio splendidus, Aliivibrio finisterrensis, Photobacterium phosphoreum and Pseudomonas fragi. Out of 340 Vibrio isolates, 238 (70 %) belonged to 21 clonal types and were found predominantly during summer when water temperatures reached 15 to 21 °C. Of these, the four major clonal types were found in multiple samples (70 %). P. fragi, on the other hand, was only found during the colder water temperatures and belonged to 18 clonal types. The presence of both groups of bacteria and their clonal types were independent of the fish diets used, suggesting that the water temperature was the main factor of the prevalence and persistence of these bacteria in the gut of Atlantic salmon.
[Mh] MeSH terms primary: Fish Diseases/microbiology
Pseudomonas Infections/veterinary
Pseudomonas/isolation & purification
Salmo salar
Seasons
Vibrio Infections/veterinary
Vibrio/isolation & purification
[Mh] MeSH terms secundary: Animals
Aquaculture
Fish Diseases/epidemiology
Molecular Sequence Data
Polymerase Chain Reaction/veterinary
Population Dynamics
Pseudomonas/classification
Pseudomonas Infections/epidemiology
Pseudomonas Infections/microbiology
RNA, Ribosomal, 16S/genetics
Random Amplified Polymorphic DNA Technique/veterinary
Sequence Analysis, DNA/veterinary
Tasmania/epidemiology
Vibrio/classification
Vibrio Infections/epidemiology
Vibrio Infections/microbiology
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (RNA, Ribosomal, 16S)
[Em] Entry month:1510
[Cu] Class update date: 170922
[Lr] Last revision date:170922
[Js] Journal subset:IM
[Da] Date of entry for processing:140717
[St] Status:MEDLINE
[do] DOI:10.1007/s00248-014-0462-x

  10 / 27 MEDLINE  
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[PMID]: 24973072
[Au] Autor:Hansen H; Bjelland AM; Ronessen M; Robertsen E; Willassen NP
[Ad] Address:The Molecular Biosystems Research Group, Department of Chemistry, Faculty of Science and Technology, UiT-The Arctic University of Norway, Tromsø, Norway Norwegian Structural Biology Centre, Faculty of Science and Technology, UiT-The Arctic University of Norway, Tromsø, Norway hilde.hansen@uit.no.
[Ti] Title:LitR is a repressor of syp genes and has a temperature-sensitive regulatory effect on biofilm formation and colony morphology in Vibrio (Aliivibrio) salmonicida.
[So] Source:Appl Environ Microbiol;80(17):5530-41, 2014 Sep.
[Is] ISSN:1098-5336
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Vibrio (Aliivibrio) salmonicida is the etiological agent of cold water vibriosis, a disease in farmed Atlantic salmon (Salmo salar) that is kept under control due to an effective vaccine. A seawater temperature below 12°C is normally required for disease development. Quorum sensing (QS) is a cell density-regulated communication system that bacteria use to coordinate activities involved in colonization and pathogenesis, and we have previously shown that inactivation of the QS master regulator LitR attenuates the V. salmonicida strain LFI1238 in a fish model. We show here that strain LFI1238 and a panel of naturally occurring V. salmonicida strains are poor biofilm producers. Inactivation of litR in the LFI1238 strain enhances medium- and temperature-dependent adhesion, rugose colony morphology, and biofilm formation. Chemical treatment and electron microscopy of the biofilm identified an extracellular matrix consisting mainly of a fibrous network, proteins, and polysaccharides. Further, by microarray analysis of planktonic and biofilm cells, we identified a number of genes regulated by LitR and, among these, were homologues of the Vibrio fischeri symbiosis polysaccharide (syp) genes. The syp genes were regulated by LitR in both planktonic and biofilm lifestyle analyses. Disruption of syp genes in the V. salmonicida ΔlitR mutant alleviated adhesion, rugose colony morphology, and biofilm formation. Hence, LitR is a repressor of syp transcription that is necessary for expression of the phenotypes examined. The regulatory effect of LitR on colony morphology and biofilm formation is temperature sensitive and weak or absent at temperatures above the bacterium's upper threshold for pathogenicity.
[Mh] MeSH terms primary: Aliivibrio salmonicida/physiology
Biofilms/growth & development
Gene Expression Regulation, Bacterial
Repressor Proteins/metabolism
[Mh] MeSH terms secundary: Aliivibrio Infections/microbiology
Aliivibrio Infections/veterinary
Aliivibrio salmonicida/genetics
Aliivibrio salmonicida/growth & development
Aliivibrio salmonicida/radiation effects
Animals
Biofilms/radiation effects
DNA, Bacterial/chemistry
DNA, Bacterial/genetics
Fish Diseases/microbiology
Gene Deletion
Gene Expression Profiling
Hemorrhagic Septicemia/microbiology
Hemorrhagic Septicemia/veterinary
Molecular Sequence Data
Polysaccharides, Bacterial/biosynthesis
Repressor Proteins/genetics
Salmo salar
Sequence Analysis, DNA
Temperature
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (DNA, Bacterial); 0 (Polysaccharides, Bacterial); 0 (Repressor Proteins)
[Em] Entry month:1504
[Cu] Class update date: 170220
[Lr] Last revision date:170220
[Js] Journal subset:IM
[Da] Date of entry for processing:140629
[St] Status:MEDLINE
[do] DOI:10.1128/AEM.01239-14


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