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| [PMID]: | 23544593 | | [Au] Autor: | Simas-Tosin FF; de Souza LM; Wagner R; Pereira GC; Barraza RR; Wendel CF; Sassaki GL; Iacomini M; Gorin PA |
| [Ad] Address: | Departamento de Bioquímica e Biologia Molecular, UFPR, CP 19046, CEP 81531-990, Curitiba, PR, Brazil. | | [Ti] Title: | Structural characterization of a glucuronoarabinoxylan from pineapple (Ananas comosus (L.) Merrill) gum exudate. | | [So] Source: | Carbohydr Polym;94(1):704-11, 2013 Apr 15. | | [Is] ISSN: | 1879-1344 | | [Cp] Country of publication: | England | | [La] Language: | eng | | [Ab] Abstract: | Native polysaccharide from pineapple gum (PANP) was obtained following alkaline extraction of gum and fractionation with cetylpyridinium chloride. It was characterized as a glucuronoarabinoxylan using NMR, methylation data, controlled Smith degradation, carboxy-reduction, and ESI-MS of oligosaccharides produced on mild acid hydrolysis of PANP. HSPEC-MALLS-RI of carboxy-reduced fraction showed homogeneous profile (Mw 1.943×10(5) g/mol). PANP was composed of Ara, Xyl, Gal, and GlcpA (40:23:7:30 molar ratio). Its main chain presented (1→4)-linked ß-xylan, highly substituted at O-2 and O-3 by side chains of 3-O- and 3,5-di-O-linked α-Araf, 2-O- and 4-O-linked α-GlcpA, and nonreducing end-units of α-Araf, ß-Arap, ß-Galp, and α-GlcpA. ESI-MS of a mixture of oligosaccharides formed on the mild acid hydrolysis of PANP was consistent with repetitive structures of α-GlcpA O-3 linked at ß-Xylp units, whereas in others glucuronoarabinoxylan-type gum exudates, α-GlcpA units had been previously found to be linked at O-2. | | [Pt] Publication type: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T | | [Em] Entry month: | 1304 | | [Js] Journal subset: | IM | | [St] Status: | In-Process |
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