Database : MEDLINE
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[PMID]: 23544593
[Au] Autor:Simas-Tosin FF; de Souza LM; Wagner R; Pereira GC; Barraza RR; Wendel CF; Sassaki GL; Iacomini M; Gorin PA
[Ad] Address:Departamento de Bioquímica e Biologia Molecular, UFPR, CP 19046, CEP 81531-990, Curitiba, PR, Brazil.
[Ti] Title:Structural characterization of a glucuronoarabinoxylan from pineapple (Ananas comosus (L.) Merrill) gum exudate.
[So] Source:Carbohydr Polym;94(1):704-11, 2013 Apr 15.
[Is] ISSN:1879-1344
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Native polysaccharide from pineapple gum (PANP) was obtained following alkaline extraction of gum and fractionation with cetylpyridinium chloride. It was characterized as a glucuronoarabinoxylan using NMR, methylation data, controlled Smith degradation, carboxy-reduction, and ESI-MS of oligosaccharides produced on mild acid hydrolysis of PANP. HSPEC-MALLS-RI of carboxy-reduced fraction showed homogeneous profile (Mw 1.943×10(5) g/mol). PANP was composed of Ara, Xyl, Gal, and GlcpA (40:23:7:30 molar ratio). Its main chain presented (1→4)-linked ß-xylan, highly substituted at O-2 and O-3 by side chains of 3-O- and 3,5-di-O-linked α-Araf, 2-O- and 4-O-linked α-GlcpA, and nonreducing end-units of α-Araf, ß-Arap, ß-Galp, and α-GlcpA. ESI-MS of a mixture of oligosaccharides formed on the mild acid hydrolysis of PANP was consistent with repetitive structures of α-GlcpA O-3 linked at ß-Xylp units, whereas in others glucuronoarabinoxylan-type gum exudates, α-GlcpA units had been previously found to be linked at O-2.
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1304
[Js] Journal subset:IM
[St] Status:In-Process

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[PMID]: 23011793
[Au] Autor:Ilaria B; Marco E; Katia L; Maria Vittoria GA
[Ad] Address:Dept. for Innovation in Biological, Agro-food and Forest systems (DIBAF), Tuscia University, via S. Camillo de Lellis, Viterbo, Italy. ilaria.be@tiscali.it
[Ti] Title:Pineapple stem bromelain immobilized on different supports: catalytic properties in model wine.
[So] Source:Biotechnol Prog;28(6):1472-7, 2012 Nov-Dec.
[Is] ISSN:1520-6033
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Bromelain from pineapple stem has been covalently immobilized on different supports to select the more efficient biocatalyst that should be applied toward unstable proteins in real white wine. In this preliminary study, catalytic properties of different immobilized bromelain forms were compared under wine-like conditions, against a synthetic substrate (Bz-Phe-Val-Arg-pNA).Covalent immobilization affected protease kinetic properties, even if all immobilized forms presented both a better substrate affinity and higher half-life (with the exception of a few procedures) with respect to the free enzyme. Stem bromelain was successfully immobilized on chitosan beads without glutaraldehyde thus yielding a food-safe and promising biocatalyst for unstable real wine future application.
[Mh] MeSH terms primary: Ananas/enzymology
Bioreactors
Bromelains/metabolism
Enzymes, Immobilized/metabolism
Wine
[Mh] MeSH terms secundary: Bromelains/chemistry
Chitosan/chemistry
Enzymes, Immobilized/chemistry
Hydrogen-Ion Concentration
Kinetics
Models, Chemical
Oligopeptides/chemistry
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Enzymes, Immobilized); 0 (Oligopeptides); 9001-00-7 (Bromelains); 9012-76-4 (Chitosan); EC 3.4.22.32 (stem bromelain)
[Em] Entry month:1305
[Js] Journal subset:IM
[Da] Date of entry for processing:121204
[St] Status:MEDLINE
[do] DOI:10.1002/btpr.1639

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[PMID]: 23064284
[Au] Autor:Kadir MF; Bin Sayeed MS; Mia MM
[Ad] Address:Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Dhaka, Dhaka 1000, Bangladesh. fakaphdu@gmail.com
[Ti] Title:Ethnopharmacological survey of medicinal plants used by indigenous and tribal people in Rangamati, Bangladesh.
[So] Source:J Ethnopharmacol;144(3):627-37, 2012 Dec 18.
[Is] ISSN:1872-7573
[Cp] Country of publication:Ireland
[La] Language:eng
[Ab] Abstract:ETHNOPHARMACOLOGICAL RELEVANCE: There is very limited information regarding plants used by traditional healers in Rangamati, Bangladesh, for treating general ailments. Current study provides significant ethnopharmacological information, both qualitative and quantitative on medical plants in Rangamati. AIM OF THE STUDY: This study aimed to collect, analyze and evaluate the rich ethnopharmacologic knowledge on medicinal plants in Rangamati and attempted to identify the important species used in traditional medicine. Further analysis was done by comparison of the traditional medicinal use with the available scientific literature data. MATERIALS AND METHODS: The field survey was carried out in a period of about one year in Rangamati, Bangladesh. A total of 152 people were interviewed, including Traditional Health Practitioners (THPs) and indigenous people through open-ended and semistructured questionnaire. The collected data were analyzed qualitatively and quantitatively. This ethnomedicinal knowledge was compared against the literature for reports of related uses and studies of phytochemical compounds responsible for respective ailments. RESULTS: A total of 144 species of plants, mostly trees, belonging to 52 families were identified for the treatment of more than 90 types of ailments. These ailments were categorized into 25 categories. Leaves were the most frequently used plant parts and decoction is the mode of preparation of major portions of the plant species. The most common mode of administration was oral ingestion and topical application. Informant consensus factor (Fic) values of the present study reflected the high agreement in the use of plants in the treatment of gastro-intestinal complaints and respiratory problems among the informants. Gastro-intestinal complaint had highest use-reports and 3 species of plants, namely Aegle marmelos (L.) Corr., Ananas comosus (L.) Merr., and Terminalia chebula (Gaertn.) Retz., had the highest fidelity level (FL) of 100%. Asparagus racemosus Willd. and Azadirachta indica A. Juss. showed the highest relative importance (RI) value of 1.86. According to use value (UV) the most important species were Azadirachta indica A. Juss. (2.48) and Ocimum sanctum L. (2.45). CONCLUSION: As a result of the present study, we recommend giving priority for further phytochemical investigation to plants that scored highest FL, Fic, UV or RI values, as such values could be considered as good indicator of prospective plants for discovering new drugs. Also counseling of THPs should be taken into consideration in order to smooth continuation and extension of traditional medical knowledge and practice for ensuring safe and effective therapy.
[Mh] MeSH terms primary: Medicine, Traditional
Plants, Medicinal/classification
[Mh] MeSH terms secundary: Adult
Bangladesh
Conservation of Natural Resources
Ethnopharmacology
Female
Health Personnel
Humans
Male
Middle Aged
Population Groups
Questionnaires
Young Adult
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1305
[Js] Journal subset:IM
[Da] Date of entry for processing:121130
[St] Status:MEDLINE

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[PMID]: 23036284
[Au] Autor:Weerahewa J; Rajapakse C; Pushpakumara G
[Ad] Address:Department of Agricultural Economics and Business Management, Faculty of Agriculture, University of Peradeniya, Sri Lanka. jeevikaw@pdn.ac.lk
[Ti] Title:An analysis of consumer demand for fruits in Sri Lanka. 1981-2010.
[So] Source:Appetite;60(1):252-8, 2013 Jan.
[Is] ISSN:1095-8304
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Micronutrient deficiency has become a serious health concern in many countries and Sri Lanka is no exception. Inclusion of vegetables and fruits, which are rich in micronutrients, in the diet is considered as one of the most cost effective measures to alleviate such deficiencies. The purpose of this paper is to analyze consumer demand for fruits in Sri Lanka. The specific objectives are to: (i) describe the patterns of fruit consumption across various households groups (ii) document the changes that have taken place in the local fruit supply for meeting the changes in demand, and (iii) to estimate price and income effects of changes in fruit consumption during 1981-2010. The study was conducted using secondary data which were extracted from government publications. The analysis shows that banana, papaw, mangoes and pineapple are the major fruits consumed and the consumption levels of such fruits have been rising over the years. The urban households and the households in high-income deciles are found to be allocating a relatively higher proportion of their food expenditure on fruits. The country is self sufficient in most of the fruits and only a small portion is traded. The results of econometric estimations reveal large income effects and relatively small price effects. A larger role for income based interventions as opposed to price based interventions to improve fruit consumption in Sri Lanka is evident from the results.
[Mh] MeSH terms primary: Diet/economics
Fruit/economics
Fruit/supply & distribution
Vegetables/economics
Vegetables/supply & distribution
[Mh] MeSH terms secundary: Ananas
Choice Behavior
Family Characteristics
Feeding Behavior
Female
Food Preferences
Food Supply/economics
Humans
Male
Mangifera
Micronutrients
Models, Econometric
Musa
Nutrition Surveys
Socioeconomic Factors
Sri Lanka
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Micronutrients)
[Em] Entry month:1305
[Js] Journal subset:IM
[Da] Date of entry for processing:121130
[St] Status:MEDLINE

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[PMID]: 22449714
[Au] Autor:Ahmed M; Laing MD; Nsahlai IV
[Ad] Address:Department of Animal Science, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, South Africa.
[Ti] Title:In vitro anthelmintic activity of crude extracts of selected medicinal plants against Haemonchus contortus from sheep.
[So] Source:J Helminthol;87(2):174-9, 2013 Jun.
[Is] ISSN:1475-2697
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Ethanol extracts of 25 plant species were screened for anthelmintic effects against Haemonchus contortus. Ethanol extracts of each plant were used at various concentrations (10, 20 and 30%) to treat 10-day faecal cultures, incubated at 27°C with control cultures which were treated with ethanol for 48 h. Five plants with high efficacies (Ananas comosus, Aloe ferox, Allium sativum, Lespedeza cuneata and Warburgia salutaris) were selected from the first screening for further investigation using ethanol, dichloromethane and water extracts at four concentrations (2.5, 5, 10 and 20%). Ethanol was the most effective solvent. Larval counts decreased with increasing extract concentrations, of which 10 and 20% had similar effects. Lespedeza cuneata caused more than 70% mortality at all concentrations. However, there remains a need to assess in vivo efficacy of these plants.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1305
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1017/S0022149X1200020X

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[PMID]: 23179718
[Au] Autor:Mhatre M
[Ad] Address:Plant Cell Culture Technology Section, Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai, India. minalmhatre@yahoo.com
[Ti] Title:Agrobacterium-mediated genetic transformation of pineapple (Ananas comosus L., Merr.).
[So] Source:Methods Mol Biol;11013:435-53, 2013.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Pineapple (Ananas comosus L., Merr.) is a commercially important crop, grown in the tropical and subtropical regions. However, the crop is faced with postharvest damage and poor varietal and nutritional improvement. Being a vegetatively propagated crop, conventional breeding programs take longer time for genetic improvement, which may not necessarily successfully develop an improved cultivar. Hence, the genetic modification of pineapple is an alternative handy approach to improve pineapple. We have established an Agrobacterium-mediated transformation system using leaf bases from in vitro-grown pineapple plants. Being a monocot, acetosyringone is added to the culture medium for overnight growth of Agrobacterium and transformation to transfer a gene of interest MSI99 soybean ferritin. Leaf bases isolated from in vitro shoot cultures are treated with Agrobacterium suspension at two dilutions, 10× and 20×, for 30 min. Explants are subsequently blot dried and cultured on gelrite solidified hormone-free Pin1 medium for 2 days (cocultivation). Periodic transfer is first done to the regeneration medium (Pin1) containing cefotaxime for the suppression of Agrobacterium growth. The transformants are selected by culturing on Pin1 medium containing cefotaxime and kanamycin. Multiple shoots, regenerated in leaf bases, are further multiplied and individually rooted in the liquid RM medium amended with antibiotics to recover plants. Putative transformants are analyzed for transgene integration and expression using standard molecular biological methods of PCR, RT-PCR, and genomic Southern.
[Mh] MeSH terms primary: Agrobacterium/genetics
Ananas/genetics
Genetic Engineering/methods
Transformation, Genetic
[Mh] MeSH terms secundary: Anti-Bacterial Agents/chemistry
Blotting, Southern
Culture Media/chemistry
DNA, Plant/genetics
DNA, Plant/isolation & purification
Ferritins/genetics
Genetic Vectors/genetics
Plasmids/genetics
Reverse Transcriptase Polymerase Chain Reaction
Soybeans/genetics
Sterilization
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Anti-Bacterial Agents); 0 (Culture Media); 0 (DNA, Plant); 9007-73-2 (Ferritins)
[Em] Entry month:1305
[Js] Journal subset:IM
[Da] Date of entry for processing:121126
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-62703-074-8_33

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[PMID]: 23122373
[Au] Autor:Kouotou EA; Defo D; Zoung-Kanyi Bissek AC; Ndjitoyap Ndam EC; Beylot-Barry M
[Ad] Address:Service de dermatologie-vénérologie, hôpital général de Yaoundé, faculté de médecine et des sciences biomédicales, BP 7132, Yaoundé, Cameroun. kouotoea@yahoo.fr
[Ti] Title:Syndrome de Stevens-Johnson après ingestion de poudre à canon. [Stevens-Johnson's syndrome following ingestion of gunpowder].
[So] Source:Ann Dermatol Venereol;139(10):617-20, 2012 Oct.
[Is] ISSN:0151-9638
[Cp] Country of publication:France
[La] Language:fre
[Ab] Abstract:BACKGROUND: A cutaneous adverse drug reaction is a skin eruption secondary to the intake of a drug, be it prescribed by a medical practitioner or taken as auto-medication for a given ailment. In this document we present an original case of Stevens-Johnson's syndrome secondary to the ingestion of gunpowder. PATIENTS AND METHODS: A 22-year-old female student was hospitalised for diffuse and painful skin eruptions for the previous three days. She had complained six days earlier of an allergic reaction to pineapples, an allergy she had presented for quite a long time. In an attempt to remedy the situation, her mother made her drink a solution made of gunpowder bought at a market mixed with some water. On the third day of this "treatment", the patient noticed eruptions on her skin. These were initially maculopapular, later becoming erosive, and she had a mild fever. Later, a variety of eruptions appeared on the skin, from hyper-pigmented macular papules to blisters and erosive lesions with no Nicolsky sign. These lesions spared the palms of the hands and the soles of the feet. The mucosa of the conjunctivae, nose, buccal cavity, vulva, vagina and anus were severely affected. This clinical presentation was typical of Steven Johnson syndrome. The patient had stopped taking the "treatment" when she noticed the first lesions. On therapy, the outcome was favourable, except for severe complications such as synechiae with diffuse dyschromia. CONCLUSION: To the best of our knowledge this is the first time gunpowder has been incriminated in Stevens-Johnson's syndrome.
[Mh] MeSH terms primary: Charcoal/toxicity
Drug Eruptions/diagnosis
Explosive Agents/toxicity
Nitrates/toxicity
Potassium Compounds/toxicity
Stevens-Johnson Syndrome/chemically induced
Sulfur/toxicity
[Mh] MeSH terms secundary: Administration, Oral
Ananas
Charcoal/administration & dosage
Drug Combinations
Drug Eruptions/pathology
Explosive Agents/administration & dosage
Female
Food Hypersensitivity/drug therapy
Humans
Nitrates/administration & dosage
Nostrums/administration & dosage
Nostrums/toxicity
Potassium Compounds/administration & dosage
Self Medication
Skin/drug effects
Skin/pathology
Stevens-Johnson Syndrome/diagnosis
Stevens-Johnson Syndrome/pathology
Sulfur/administration & dosage
Young Adult
[Pt] Publication type:CASE REPORTS; ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Name of substance:0 (Drug Combinations); 0 (Explosive Agents); 0 (Nitrates); 0 (Nostrums); 0 (Potassium Compounds); 16291-96-6 (Charcoal); 7704-34-9 (Sulfur); RU45X2JN0Z (potassium nitrate)
[Em] Entry month:1305
[Js] Journal subset:IM
[Da] Date of entry for processing:121105
[St] Status:MEDLINE

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[PMID]: 23040047
[Au] Autor:Echeverría-Sáenz S; Mena F; Pinnock M; Ruepert C; Solano K; de la Cruz E; Campos B; Sánchez-Avila J; Lacorte S; Barata C
[Ad] Address:Central American Institute for Studies on Toxic Substances/Instituto Regional de Estudios en Sustancias Tóxicas (IRET), Universidad Nacional, Campus Omar Dengo, Heredia, Costa Rica.
[Ti] Title:Environmental hazards of pesticides from pineapple crop production in the Río Jiménez watershed (Caribbean Coast, Costa Rica).
[So] Source:Sci Total Environ;440:106-14, 2012 Dec 1.
[Is] ISSN:1879-1026
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:This study aimed to characterize environmental hazards of pesticides from pineapple production in riparian communities along the Jiménez River watershed. To achieve our objectives riparian ecological quality indices on riparian habitat and macroinvertebrate assemblages were combined with toxicity assays, fish biomarkers, physico-chemical water analysis and pesticide environmental hazards. During two consecutive years and two periods (July and October), three reference and four impacted sites were monitored. The ecological quality of benthic macroinvertebrates and of riparian habitats deteriorated from the reference sites downstream to the polluted reaches along the Jiménez River area affected by pineapple plantations. The toxicity of water to Daphnia magna also increased towards downstream reaches. Biomarkers of fish of the species Poecilia gillii and Bryconamericus scleroparius transplanted across the studied sites evidenced a clear anticholinergic effect towards downstream sites as well as increased levels of lipid peroxidation. Different pesticide residues were frequently detected in water samples collected across the Jiménez River watershed with herbicides (ametryn, bromacil, diuron), organophosphorus insecticides (diazinon and ethoprophos) and triazole fungicides being the greatest reaching levels above 1 µg L(-1) in downstream sites. Principal component and environmental hazard analysis of physico-chemical and biological responses established clear relationships among habitat deterioration and the ecological quality of macroinvertebrate communities, high levels of herbicides and poor plant growth, high levels of organophosphorus insecticides in water and anticholinesterase effects on fish, D. magna mortality and deterioration of macroinvertebrate communities. Fungicide and herbicide residue levels were also related with high levels of lipid peroxidation and high activities of glutathione S transferase in fish liver, respectively. These results indicated, thus, that riparian habitat deterioration due to deforestation and land use for agriculture and pesticide contamination are affecting river ecosystems.
[Mh] MeSH terms primary: Environmental Exposure
Pesticide Residues/toxicity
Pesticides/toxicity
Water Pollutants, Chemical/toxicity
[Mh] MeSH terms secundary: Agriculture
Ananas
Animals
Cholinesterases/metabolism
Chromatography, Liquid
Costa Rica
Daphnia/drug effects
Environmental Monitoring
Fishes/metabolism
Gas Chromatography-Mass Spectrometry
Glutathione Transferase/metabolism
Lipid Peroxidation
Pesticide Residues/analysis
Pesticides/analysis
Plants/drug effects
Rivers
Seasons
Solid Phase Extraction
Tandem Mass Spectrometry
Water Pollutants, Chemical/analysis
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Pesticide Residues); 0 (Pesticides); 0 (Water Pollutants, Chemical); EC 2.5.1.18 (Glutathione Transferase); EC 3.1.1.8 (Cholinesterases)
[Em] Entry month:1304
[Js] Journal subset:IM
[Da] Date of entry for processing:121112
[St] Status:MEDLINE

  9 / 323 MEDLINE  
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[PMID]: 23139757
[Au] Autor:Irene D; Chung TY; Chen BJ; Liu TH; Li FY; Tzen JT; Wang CI; Chyan CL
[Ad] Address:Department of Chemistry, National Dong Hwa University, Hualien, Taiwan, Republic of China.
[Ti] Title:Solution structure of a phytocystatin from Ananas comosus and its molecular interaction with papain.
[So] Source:PLoS One;7(11):e47865, 2012.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The structure of a recombinant pineapple cystatin (AcCYS) was determined by NMR with the RMSD of backbone and heavy atoms of twenty lowest energy structures of 0.56 and 1.11 Å, respectively. It reveals an unstructured N-terminal extension and a compact inhibitory domain comprising a four-stranded antiparallel ß-sheet wrapped around a central α-helix. The three structural motifs (G(45), Q(89)XVXG, and W(120)) putatively responsible for the interaction with papain-like proteases are located in one side of AcCYS. Significant chemical shift perturbations in two loop regions, residues 45 to 48 (GIYD) and residues 89 to 91 (QVV), of AcCYS strongly suggest their involvement in the binding to papain, consistent with studies on other members of the cystatin family. However, the highly conserved W120 appears not to be involved in the binding with papain as no chemical shift perturbation was observed. Chemical shift index analysis further indicates that the length of the α-helix is shortened upon association with papain. Collectively, our data suggest that AcCYS undergoes local secondary structural rearrangements when papain is brought into close contact. A molecular model of AcCYS/papain complex is proposed to illustrate the interaction between AcCYS and papain, indicating a complete blockade of the catalytic triad by AcCYS.
[Mh] MeSH terms primary: Ananas/metabolism
Cystatins/chemistry
Cystatins/metabolism
Papain/metabolism
[Mh] MeSH terms secundary: Amino Acid Sequence
Circular Dichroism
Deuterium Exchange Measurement
Hydrophobic and Hydrophilic Interactions
Kinetics
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Structure, Secondary
Protein Unfolding
Sequence Alignment
Solutions
Thermodynamics
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Cystatins); 0 (Solutions); EC 3.4.22.2 (Papain)
[Em] Entry month:1304
[Js] Journal subset:IM
[Da] Date of entry for processing:121109
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0047865

  10 / 323 MEDLINE  
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[PMID]: 23263857
[Au] Autor:Koia J; Moyle R; Hendry C; Lim L; Botella JR
[Ad] Address:University of Queensland, Brisbane, 4072, Australia.
[Ti] Title:Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana.
[So] Source:Plant Mol Biol;81(4-5):327-36, 2013 Mar.
[Is] ISSN:1573-5028
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:The availability of a variety of promoter sequences is necessary for the genetic engineering of plants, in basic research studies and for the development of transgenic crops. In this study, the promoter and 5' untranslated regions of the evolutionally conserved protein translation factor SUI1 gene and ribosomal protein L36 gene were isolated from pineapple and sequenced. Each promoter was translationally fused to the GUS reporter gene and transformed into the heterologous plant system Arabidopsis thaliana. Both the pineapple SUI1 and L36 promoters drove GUS expression in all tissues of Arabidopsis at levels comparable to the CaMV35S promoter. Transient assays determined that the pineapple SUI1 promoter also drove GUS expression in a variety of climacteric and non-climacteric fruit species. Thus the pineapple SUI1 and L36 promoters demonstrate the potential for using translation factor and ribosomal protein genes as a source of promoter sequences that can drive constitutive transgene expression patterns.
[Mh] MeSH terms primary: Ananas/genetics
Arabidopsis/genetics
Gene Expression Regulation, Plant
Plant Proteins/genetics
Promoter Regions, Genetic/genetics
Transgenes/genetics
[Mh] MeSH terms secundary: Ananas/metabolism
Base Sequence
Eukaryotic Initiation Factors/genetics
Eukaryotic Initiation Factors/metabolism
Fluorometry
Fruit/genetics
Glucuronidase/metabolism
Plant Proteins/metabolism
Plants, Genetically Modified
Real-Time Polymerase Chain Reaction
Ribosomal Proteins/genetics
Ribosomal Proteins/metabolism
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Eukaryotic Initiation Factors); 0 (Plant Proteins); 0 (Ribosomal Proteins); EC 3.2.1.31 (Glucuronidase)
[Em] Entry month:1304
[Js] Journal subset:IM
[Da] Date of entry for processing:130225
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-012-0002-3


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