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[PMID]: 29524794
[Au] Autor:Kubiasová K; Mik V; Nisler J; Hönig M; Husicková A; Spíchal L; Pekná Z; Samajová O; Dolezal K; Plíhal O; Benková E; Strnad M; Plíhalová L
[Ad] Address:Department of Molecular Biology, Centre of the Region Haná for Biotechnological and Agricultural Research, Slechtitelu 27, Olomouc 783 71, Czech Republic.
[Ti] Title:Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins.
[So] Source:Phytochemistry;150:1-11, 2018 Mar 07.
[Is] ISSN:1873-3700
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Isoprenoid cytokinins play a number of crucial roles in the regulation of plant growth and development. To study cytokinin receptor properties in plants, we designed and prepared fluorescent derivatives of 6-[(3-methylbut-2-en-1-yl)amino]purine (N -isopentenyladenine, iP) with several fluorescent labels attached to the C2 or N9 atom of the purine moiety via a 2- or 6-carbon linker. The fluorescent labels included dansyl (DS), fluorescein (FC), 7-nitrobenzofurazan (NBD), rhodamine B (RhoB), coumarin (Cou), 7-(diethylamino)coumarin (DEAC) and cyanine 5 dye (Cy5). All prepared compounds were screened for affinity for the Arabidopsis thaliana cytokinin receptor (CRE1/AHK4). Although the attachment of the fluorescent labels to iP via the linkers mostly disrupted binding to the receptor, several fluorescent derivatives interacted well. For this reason, three derivatives, two rhodamine B and one 4-chloro-7-nitrobenzofurazan labeled iP were tested for their interaction with CRE1/AHK4 and Zea mays cytokinin receptors in detail. We further showed that the three derivatives were able to activate transcription of cytokinin response regulator ARR5 in Arabidopsis seedlings. The activity of fluorescently labeled cytokinins was compared with corresponding 6-dimethylaminopurine fluorescently labeled negative controls. Selected rhodamine B C2-labeled compounds 17, 18 and 4-chloro-7-nitrobenzofurazan N9-labeled compound 28 and their respective negative controls (19, 20 and 29, respectively) were used for in planta staining experiments in Arabidopsis thaliana cell suspension culture using live cell confocal microscopy.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  2 / 63319 MEDLINE  
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[PMID]: 29524407
[Au] Autor:Yu CY; Nguyen VC; Chuang L; Kanehara K
[Ad] Address:Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan; Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica and National Chung Hsing University, Taipei 11529, Taiwan; Graduate Institute of Biotechnology, National Chu
[Ti] Title:Membrane glycerolipid equilibrium under endoplasmic reticulum stress in Arabidopsis thaliana.
[So] Source:Biochem Biophys Res Commun;, 2018 Mar 07.
[Is] ISSN:1090-2104
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Eendoplasmic reticulum (ER) is an indispensable organelle for secretory protein synthesis as well as metabolism of phospholipids and their derivatives in eukaryotic cells. Various external and internal factors may cause an accumulation of aberrant proteins in the ER, which causes ER stress and activates cellular ER stress responses to cope with the stress. In animal research, molecular mechanisms for protein quality control upon ER stress are well documented, but how cells maintain lipid homeostasis under ER stress is an emerging issue. The ratio of phosphatidylcholine (PC) to phosphatidylethanolamine (PE), two major phospholipid classes, is important under ER stress in animal cells. However, no study of plants has reported on the changes in membrane lipid content under ER stress, although a number of physiologically important environmental stresses, such as heat and salinity, induce ER stress in plants. Here, we investigated membrane glycerolipid metabolism under ER stress in Arabidopsis. ER stress transcriptionally affected PC and PE biosynthesis pathways differentially, with no significant changes in membrane glycerolipid content. Our results suggest that higher plants maintain membrane lipid equilibrium during active transcription of biosynthetic genes under ER stress.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  3 / 63319 MEDLINE  
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[PMID]: 29510200
[Au] Autor:Zhang ZW; Li MX; Huang B; Feng LY; Wu F; Fu YF; Zheng XJ; Peng HQ; Chen YE; Yang HN; Wu LT; Yuan M; Yuan S
[Ad] Address:College of Resources, Sichuan Agricultural University, Chengdu 611130, China.
[Ti] Title:Nitric oxide regulates chlorophyllide biosynthesis and singlet oxygen generation differently between Arabidopsis and barley.
[So] Source:Nitric Oxide;76:6-15, 2018 Mar 03.
[Is] ISSN:1089-8611
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Nitric oxide (NO) has a general inhibitory effects on chlorophyll biosynthesis, especially to the step of 5-aminolevulinic acid (ALA) biosynthesis and protochlorophyllide (Pchlide) to chlorophyllide (Chlide) conversion (responsible by the NADPH:Pchlide oxidoreductase POR). Previous study suggested that barley large POR aggregates may be generated by dithiol oxidation of cysteines of two POR monomers, which can be disconnected by some reducing agents. POR aggregate assembly may be correlated with seedling greening in barley, but not in Arabidopsis. Thus, NO may affect POR activity and seedling greening differently between Arabidopsis and barley. We proved this assumption by non-denaturing gel-analysis and reactive oxygen species (ROS) monitoring during the greening. NO treatments cause S-nitrosylation to POR cysteine residues and disassembly of POR aggregates. This modification reduces POR activity and induces Pchlide accumulation and singlet oxygen generation upon dark-to-high-light shift (and therefore inducing photobleaching lesions) in barley leaf apex, but not in Arabidopsis seedlings. ROS staining and ROS-related-gene expression detection confirmed that superoxide anion and singlet oxygen accumulated in barley etiolated seedlings after the NO treatments, when exposed to a fluctuating light. The data suggest that POR aggregate assembly may be correlated with barley chlorophyll biosynthesis and redox homeostasis during greening. Cysteine S-nitrosylation may be one of the key reasons for the NO-induced inhibition to chlorophyll biosynthetic enzymes.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  4 / 63319 MEDLINE  
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[PMID]: 29499229
[Au] Autor:Chumová J; Trögelová L; Kourová H; Volc J; Sulimenko V; Halada P; Kucera O; Benada O; Kucharová A; Klebanovych A; Dráber P; Daniel G; Binarová P
[Ad] Address:Institute of Microbiology of the Czech Academy of Sciences, Vídenská 1083, 142 20 Prague 4, Czech Republic.
[Ti] Title:γ-Tubulin has a conserved intrinsic property of self-polymerization into double stranded filaments and fibrillar networks.
[So] Source:Biochim Biophys Acta;1865(5):734-748, 2018 Feb 27.
[Is] ISSN:0006-3002
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:γ-Tubulin is essential for microtubule nucleation and also plays less understood roles in nuclear and cell-cycle-related functions. High abundancy of γ-tubulin in acentrosomal Arabidopsis cells facilitated purification and biochemical characterization of large molecular species of γ-tubulin. TEM, fluorescence, and atomic force microscopy of purified high molecular γ-tubulin forms revealed the presence of linear filaments with a double protofilament substructure, filament bundles and aggregates. Filament formation from highly purified γ-tubulin free of γ-tubulin complex proteins (GCPs) was demonstrated for both plant and human γ-tubulin. Moreover, γ-tubulin associated with porcine brain microtubules formed oligomers. Experimental evidence on the intrinsic ability of γ-tubulin to oligomerize/polymerize was supported by conservation of α- and ß-tubulin interfaces for longitudinal and lateral interactions for γ-tubulins. STED (stimulated emission depletion) microscopy of Arabidopsis cells revealed fine, short γ-tubulin fibrillar structures enriched on mitotic microtubular arrays that accumulated at polar regions of acentrosomal spindles and the outer nuclear envelope before mitosis, and were also present in nuclei. Fine fibrillar structures of γ-tubulin representing assemblies of higher order were localized in cell-cycle-dependent manner at sites of dispersed γ-tubulin location in acentrosomal plant cells as well as at sites of local γ-tubulin enrichment after drug treatment. Our findings that γ-tubulin preserves the capability of prokaryotic tubulins to self-organize into filaments assembling by lateral interaction into bundles/clusters help understanding of the relationship between structure and multiple cellular functions of this protein species and suggest that besides microtubule nucleation and organization, γ-tubulin may also have scaffolding or sequestration functions.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  5 / 63319 MEDLINE  
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[PMID]: 29482161
[Au] Autor:Yang Q; Wang L; He J; Yang Z; Huang X
[Ad] Address:National and Local Joint Engineering Research Center of Biomedical Functional Materials, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Sciences, Nanjing Normal University, Nanjing 210046, China.
[Ti] Title:Direct imaging of how lanthanides break the normal evolution of plants.
[So] Source:J Inorg Biochem;182:158-169, 2018 Feb 10.
[Is] ISSN:1873-3344
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:After rare earth elements [REE(III)] are anchored outside of the plasma membrane, REE(III) break plant evolution to initiate leaf cell endocytosis, which finally affects plant growth. However, the molecule for anchoring REE(III) in the acidic environment outside of the plasma membrane is not clear, which is crucial for exploring the mechanism of REE(III) breaking plant evolution. Here, lanthanum(III) [La(III)] and terbium(III) [Tb(III)] were respectively served as a representative of REE(III) without and with f electrons, and Arabidopsis was served as a representative of plants, cellular and molecular basis for arabinogalactan proteins (AGP) anchoring REE(III) outside of the plasma membrane was investigated. By using interdisciplinary methods, when REE(III) initiated leaf cell phagocytosis, we observed the increase in the expression of AGP and their migration to the outside of the plasma membrane. In the acidic environment outside of the plasma membrane, Tb(III) formed more stable Lewis acid-base [REE(III)-AGP] complexes with a higher apparent binding constant (1.51 × 10 ) than La(III) (1.24 × 10 ). In REE(III)-AGP complexes, the bond lengths of REE(III)-O were in normal range and H-bonds were strong H-bonds. The formation of REE(III)-AGP complexes sequentially disturbed the secondary and tertiary structure of AGP, which were enhanced with increasing the concentration of REE(III), and Tb(III) caused stronger structural changes than La(III). Hence, AGP could be molecules for anchoring REE(III) outside of the plasma membrane. The results of this study are direct imaging of how lanthanides break the normal evolution of plants, and can serve as an important guidance for investigating mechanism of lanthanides in organisms.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  6 / 63319 MEDLINE  
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[PMID]: 29523961
[Au] Autor:Peng F; Wang C; Zhu J; Zeng J; Kang H; Fan X; Sha L; Zhang H; Zhou Y; Wang Y
[Ad] Address:Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Sichuan, China.
[Ti] Title:Expression of TpNRAMP5, a metal transporter from Polish wheat (Triticum polonicum L.), enhances the accumulation of Cd, Co and Mn in transgenic Arabidopsis plants.
[So] Source:Planta;, 2018 Mar 09.
[Is] ISSN:1432-2048
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:MAIN CONCLUSION: TpRNAMP5 is mainly expressed in the plasma membrane of roots and basal stems. It functions as a metal transporter for Cd, Mn and Co accumulation. Numerous natural resistance-associated macrophage proteins (NRAMPs) have been functionally identified in various plant species, including Arabidopsis, rice, soybean and tobacco, but no information is available on NRAMP genes in wheat. In this study, we isolated a TpNRAMP5 from dwarf Polish wheat (DPW, Triticum polonicum L.), a species with high tolerance to Cd and Zn. Expression pattern analysis revealed that TpNRAMP5 is mainly expressed in roots and basal stems of DPW. TpNRAMP5 was localized at the plasma membrane of Arabidopsis leaf protoplast. Expression of TpNRAMP5 in yeast significantly increased yeast sensitivity to Cd and Co, but not Zn, and enhanced Cd and Co concentrations. Expression of TpNRAMP5 in Arabidopsis significantly increased Cd, Co and Mn concentrations in roots, shoots and whole plants, but had no effect on Fe and Zn concentrations. These results indicate that TpNRAMP5 is a metal transporter enhancing the accumulation of Cd, Co and Mn, but not Zn and Fe. Genetic manipulation of TpNRAMP5 can be applied in the future to limit the transfer of Cd from soil to wheat grains, thereby protecting human health.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher
[do] DOI:10.1007/s00425-018-2872-3

  7 / 63319 MEDLINE  
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[PMID]: 29523715
[Au] Autor:Kumar M; Mishra L; Carr P; Pilling M; Gardner P; Mansfield SD; Turner SR
[Ad] Address:University of Manchester CITY: Manchester United Kingdom [GB].
[Ti] Title:Exploiting CELLULOSE SYNTHASE (CESA) class-specificity to probe cellulose microfibril biosynthesis.
[So] Source:Plant Physiol;, 2018 Mar 09.
[Is] ISSN:1532-2548
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Cellulose microfibrils are the basic units of cellulose in plants. The structure of these microfibrils is at least partly determined by the structure of the cellulose synthase complex. In higher plants, this complex is composed of 18 to 24 catalytic subunits known as CELLULOSE SYNTHASE A (CESA) proteins. Three different classes of CESA proteins are required for cellulose synthesis and for secondary cell wall cellulose biosynthesis, which include CESA4, CESA7, and CESA8. To probe the relationship between CESA proteins and microfibril structure, we created mutant cesa proteins that lack catalytic activity but retain sufficient structural integrity to allow assembly of the cellulose synthase complex. Using a series of Arabidopsis mutants and genetic backgrounds, we found consistent differences in the ability of these mutant CESA proteins to complement the cellulose-deficient phenotype of the cesa null mutants. The best complementation was observed with catalytically inactive cesa4 while the equivalent mutation in cesa8 exhibited significantly lower levels of complementation. Using a variety of biophysical techniques, including ssNMR and FTIR, to study these mutant plants we found evidence for changes in cellulose microfibril structure, but these changes largely correlated with cellulose content and reflected differences in the relative proportion of primary and secondary cell walls. Our results suggest that individual CESA classes have similar roles in determining cellulose microfibril structure, and it is likely that the different effects of mutating members of different CESA classes is a consequence of their different catalytic activity and their influence on the overall rate of cellulose synthesis.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  8 / 63319 MEDLINE  
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[PMID]: 29523714
[Au] Autor:Höhner R; Marques JV; Ito T; Amakura Y; Budgeon AD; Weitz K; Hixson KK; Davin LB; Kirchhoff H; Lewis NG
[Ad] Address:Washington State University CITY: Pullman STATE: Washington United States Of America [US].
[Ti] Title:Reduced Arogenate Dehydratase Expression: Ramifications for Photosynthesis and Metabolism.
[So] Source:Plant Physiol;, 2018 Mar 09.
[Is] ISSN:1532-2548
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Arogenate dehydratase (ADT) catalyzes the final step of phenylalanine (Phe) biosynthesis. Previous work showed that ADT-deficient Arabidopsis thaliana mutants had significantly reduced lignin contents, with stronger reductions in lines that had deficiencies in more ADT isoforms. Here, by analyzing Arabidopsis ADT mutants using our phenomics facility and UPLC-MS based metabolomics, we describe the effects of modulation of ADT on photosynthetic parameters and secondary metabolism. Our data indicate that a reduced carbon flux into Phe biosynthesis in ADT mutants impairs the consumption of photosynthetically produced ATP leading to an increased ATP/ADP ratio, the over-accumulation of transitory starch, and lower electron transport rates. The effect on electron transport rates is caused by an increase in proton motive force across the thylakoid membrane that down-regulates Photosystem II activity by the high energy quenching mechanism. Furthermore, quantitation of secondary metabolites in ADT mutants revealed reduced flavonoid, phenylpropanoid, lignan, and glucosinolate contents, including glucosinolates that are not derived from aromatic amino acids, and significantly increased contents of putative galactolipids and apocarotenoids. Additionally, we used real-time atmospheric monitoring mass spectrometry to compare respiration and carbon fixation rates between wild-type and adt3/4/5/6, our most extreme ADT knock-out mutant, which revealed no significant difference in both night- and day-adapted plants. Overall, these data reveal the profound effects of altered ADT activity and Phe metabolism on secondary metabolites and photosynthesis with implications for plant improvement.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  9 / 63319 MEDLINE  
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[PMID]: 29523713
[Au] Autor:Law SR; Chrobok D; Juvany M; Delhomme N; Lindén P; Brouwer B; Ahad A; Moritz T; Jansson S; Gardestrom P; Keech O
[Ad] Address:Umeå Plant Science Centre, Umeå University CITY: Umeå Sweden [SE].
[Ti] Title:Darkened leaves use different metabolic strategies for senescence and survival.
[So] Source:Plant Physiol;, 2018 Mar 09.
[Is] ISSN:1532-2548
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:In plants, an individually darkened leaf (IDL) initiates senescence much more rapidly than a leaf from a whole darkened plant (DP). Combining transcriptomic and metabolomic approaches in Arabidopsis thaliana, we present an overview of the metabolic strategies that are employed in response to different darkening treatments. Under DP conditions, the perception of carbon starvation drove a profound metabolic readjustment in which lipids, branched-chain amino acids, and potentially monosaccharides released from cell wall loosening became important substrates for maintaining minimal ATP production. Concomitantly, the increased accumulation of amino acids with a high nitrogen:carbon ratio may provide a safety mechanism for the storage of metabolically-derived cytotoxic ammonium and a pool of nitrogen for use upon returning to typical growth conditions. Conversely, in IDL, the metabolic profiling that followed our 13C enrichment assays revealed a temporal and differential exchange of metabolites, including sugars and amino acids, between the darkened leaf and the rest of the plant. This active transport could be the basis for a progressive metabolic shift in the substrates fuelling mitochondrial activities, which are central to the catabolic reactions facilitating the retrieval of nutrients from the senescing leaf. We propose a model illustrating the specific metabolic strategies employed by leaves in response to these two darkening treatments, which either support rapid senescence or a strong capacity for survival.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  10 / 63319 MEDLINE  
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[PMID]: 29523712
[Au] Autor:Feng QN; Liang X; Li S; Zhang Y
[Ad] Address:State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University CITY: Tai'an China [CN].
[Ti] Title:The ADAPTOR PROTEIN-3 complex mediates pollen tube growth by coordinating vacuolar targeting and organization.
[So] Source:Plant Physiol;, 2018 Mar 09.
[Is] ISSN:1532-2548
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Pollen tube growth is an essential step for successful plant reproduction. Vacuolar trafficking and dynamic organization are important for pollen tube growth; however, the key proteins involved in these processes are not well understood. Here, we report that the ADAPTOR PROTEIN-3 (AP-3) complex and its tonoplast cargo PROTEIN S-ACYL TRANSFERASE10 (PAT10) are critical for pollen tube growth in Arabidopsis (Arabidopsis thaliana). AP-3 is a heterotetrameric protein complex consisting of four subunits, δ, ß, µ, and σ. AP-3 regulates tonoplast targeting of several cargoes, such as PAT10. We show that functional loss of any of the four AP-3 subunits reduces plant fertility. In ap-3 mutants, pollen development was normal but pollen tube growth was compromised, leading to reduced male transmission. Functional loss of PAT10 caused a similar reduction in pollen tube growth, suggesting that the tonoplast association of PAT10 mediated by AP-3 is crucial for this process. Indeed, Ca2+ gradient during pollen tube growth was significantly reduced due to AP-3 loss-of-function, consistent with the abnormal targeting of Calcinuerin B-like2 (CBL2) and CBL3, whose tonoplast association depends on PAT10. Further, we show that the pollen tubes of ap-3 mutants have vacuoles with simplified tubules and bulbous structures, indicating that AP-3 affects vacuolar organization. Our results demonstrate a role for AP-3 in plant reproduction and provide insights into the role of vacuoles in polarized cell growth.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher


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