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[PMID]: 25336340
[Au] Autor:Auletta JJ; Eid SK; Wuttisarnwattana P; Silva I; Metheny L; Keller MD; Guardia-Wolff R; Liu C; Wang F; Bowen T; Lee Z; Solchaga LA; Ganguly S; Tyler M; Wilson DL; Cooke KR
[Ad] Address:Host Defense Program, Hematology/Oncology/BMT and Infectious Diseases, Nationwide Children's Hospital, Columbus, Ohio, USA; Department of Pediatrics, The Ohio State University, Columbus, Ohio, USA.
[Ti] Title:Human mesenchymal stromal cells attenuate graft-versus-host disease and maintain graft-versus-leukemia activity following experimental allogeneic bone marrow transplantation.
[So] Source:Stem Cells;33(2):601-14, 2015 Feb.
[Is] ISSN:1549-4918
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:We sought to define the effects and underlying mechanisms of human, marrow-derived mesenchymal stromal cells (hMSCs) on graft-versus-host disease (GvHD) and graft-versus-leukemia (GvL) activity. Irradiated B6D2F1 mice given C57BL/6 BM and splenic T cells and treated with hMSCs had reduced systemic GvHD, donor T-cell expansion, and serum TNFα and IFNγ levels. Bioluminescence imaging demonstrated that hMSCs redistributed from lungs to abdominal organs within 72 hours, and target tissues harvested from hMSC-treated allogeneic BMT (alloBMT) mice had less GvHD than untreated controls. Cryoimaging more precisely revealed that hMSCs preferentially distributed to splenic marginal zones and regulated T-cell expansion in the white pulp. Importantly, hMSCs had no effect on in vitro cytotoxic T-cell activity and preserved potent GvL effects in vivo. Mixed leukocyte cultures containing hMSCs exhibited decreased T-cell proliferation, reduced TNFα, IFNγ, and IL-10 but increased PGE2 levels. Indomethacin and E-prostanoid 2 (EP2) receptor antagonisms both reversed while EP2 agonism restored hMSC-mediated in vitro T-cell suppression, confirming the role for PGE2 . Furthermore, cyclo-oxygenase inhibition following alloBMT abrogated the protective effects of hMSCs. Together, our data show that hMSCs preserve GvL activity and attenuate GvHD and reveal that hMSC biodistribute to secondary lymphoid organs wherein they attenuate alloreactive T-cell proliferation likely through PGE2 induction. Stem Cells 2015;33:601-614.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1002/stem.1867

  2 / 316055 MEDLINE  
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[PMID]: 25331517
[Au] Autor:Akkiraju H; Bonor J; Olli K; Bowen C; Bragdon B; Coombs H; Donahue LR; Duncan R; Nohe A
[Ad] Address:Department of Biological Sciences, University of Delaware, Newark, Delaware.
[Ti] Title:Systemic Injection of CK2.3, a Novel Peptide Acting Downstream of Bone Morphogenetic Protein Receptor BMPRIa, Leads to Increased Trabecular Bone Mass.
[So] Source:J Orthop Res;33(2):208-15, 2015 Feb.
[Is] ISSN:1554-527X
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Bone Morphogenetic Protein 2 (BMP2) regulates bone integrity by driving both osteogenesis and osteoclastogenesis. However, BMP2 as a therapeutic has significant drawbacks. We have designed a novel peptide CK2.3 that blocks the interaction of Casein Kinase 2 (CK2) with Bone Morphogenetic Protein Receptor type Ia (BMPRIa), thereby activating BMP signaling pathways in the absence of ligand. Here, we show that CK2.3 induced mineralization in primary osteoblast cultures isolated from calvaria and bone marrow stromal cells (BMSCs) of 8 week old mice. Further, systemic tail vein injections of CK2.3 in 8 week old mice resulted in increased bone mineral density (BMD) and mineral apposition rate (MAR). In situ immunohistochemistry of the femur found that CK2.3 injection induced phosphorylation of extracellular signal-related kinase (ERK), but not Smad in osteocytes and osteoblasts, suggesting that CK2.3 signaling occurred through Smad independent pathway. Finally mice injected with CK2.3 exhibited decreased osteoclast differentiation and osteoclast activity. These data indicate that the novel mimetic peptide CK2.3 activated BMPRIa downstream signaling to enhance bone formation without the increase in osteoclast activity that accompanies BMP 2 stimulation. 2014 Orthopaedic Research Society. 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:208-215, 2015.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1002/jor.22752

  3 / 316055 MEDLINE  
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[PMID]: 25610471
[Au] Autor:Qi C; Xiaofeng X; Xiaoguang W
[Ad] Address:Department of Orthopedics, Affiliated Hospital of Jiangsu University, 438 Jiefang Road, Zhenjiang, Jiangsu 212000, China.
[Ti] Title:Effects of toll-like receptors 3 and 4 in the osteogenesis of stem cells.
[So] Source:Stem Cells Int;2014:917168, 2014.
[Is] ISSN:1687-966X
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Objective. To investigate the effects of Toll-like receptors in stem cell osteogenesis. Methods. Bone marrow mesenchymal stem cells (BMSCs) were divided into the blank group, the TLR-3 activated group, and the TLR-4 activated group. After 10 days' osteogenic-promoting culture, expression of type I collagen and osteocalcin was determined by Western blot. Osteoblasts (OBs) were also divided into three groups mentioned above. Alkaline phosphatase (ALP) and alizarin red staining were performed after 10 days' ossification-inducing culture. The expression of -catenin was investigated by Western blot. Results. Both the TLR-3 and TLR-4 activated groups had increased expression of type I collagen and osteocalcin; the effect of TLR-4 was stronger. The intensity of alizarin red and ALP staining was strongest in the TLR-3 activated group and weakest in the TLR-4 activated group. Activation of TLR-4 decreased the expression of -catenin, whilst activation of TLR-3 did not affect the expression of -catenin. Discussion. This study suggested that both TLR-3 and -4 promoted differentiation of BMSCs to OBs. TLR-3 had an inducing effect on the ossification of OBs to osteocytes, whilst the effect of TLR-4 was the opposite because of its inhibitory effect on the Wnt signaling pathway.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Da] Date of entry for processing:150122
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1155/2014/917168

  4 / 316055 MEDLINE  
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[PMID]: 25609962
[Au] Autor:Lai GJ; Shalumon K; Chen JP
[Ad] Address:Department of Chemical and Materials Engineering, Chang Gung University of Science and Technology, Taoyuan, Taiwan, Republic of China.
[Ti] Title:Response of human mesenchymal stem cells to intrafibrillar nanohydroxyapatite content and extrafibrillar nanohydroxyapatite in biomimetic chitosan/silk fibroin/nanohydroxyapatite nanofibrous membrane scaffolds.
[So] Source:Int J Nanomedicine;10:567-84, 2015.
[Is] ISSN:1178-2013
[Cp] Country of publication:New Zealand
[La] Language:eng
[Ab] Abstract:Incorporation of nanohydroxyapatite (nHAP) within a chitosan (CS)/silk fibroin (SF) nanofibrous membrane scaffold (NMS) may provide a favorable microenvironment that more closely mimics the natural bone tissue physiology and facilitates enhanced osteogensis of the implanted cell population. In this study, we prepared pristine CS/SF NMS, composite CS/SF/nHAP NMS containing intrafibrillar nHAP by in situ blending of 10% or 30% nHAP before the electrospinning step, and composite CS/SF/nHAP NMS containing extrafibrillar nHAP by depositing 30% nHAP through alternative soaking surface mineralization. We investigated the effect of the incorporation of HAP nanoparticles on the physicochemical properties of pristine and composite NMS. We confirmed the presence of ~30 nm nHAP in the composite nanofibrous membranes by thermogravimetry analysis (TGA), X-ray diffraction (XRD), and scanning electron microscopy (SEM), either embedded in or exposed on the nanofiber. Nonetheless, the alternative soaking surface mineralization method drastically influenced the mechanical properties of the NMS with 88% and 94% drop in Young's modulus and ultimate maximum stress. Using in vitro cell culture experiments, we investigated the effects of nHAP content and location on proliferation and osteogenic differentiation of human bone marrow mesenchymal stem cells (hMSCs). The proliferation of hMSCs showed no significant difference among pristine and composite NMS. However, the extent of osteogenic differentiation of hMSCs was found to be positively correlated with the content of nHAP in the NMS, while its location within the nanofiber played a less significant role. In vivo experiments were carried out with hMSCs seeded in CS/SF/30%nHAP NMS prepared by in situ blending and subcutaneous implantation in nude mice. Micro-computed tomography images as well as histological and immunohistochemical analysis of the retrieved hMSCs/NMS construct 1 and 2 months postimplantation indicated that NMS had the potential for bone regeneration and can be suggested as a promising scaffold for bone tissue engineering.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.2147/IJN.S73780

  5 / 316055 MEDLINE  
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[PMID]: 25610688
[Au] Autor:akan M; Ko A; Cerit K; Bozkurt S; Ergelen R; Vural I
[Ad] Address:Department of Pediatric Hematology and Oncology, Faculty of Medicine, Marmara University, Mimar Sinan Caddesi No. 41, Pendik, 34899 Istanbul, Turkey....
[Ti] Title:A Case of Acute Myeloid Leukemia (FAB M2) with Inversion 16 Who Presented with Pelvic Myeloid Sarcoma.
[So] Source:Case Rep Pediatr;2014:246169, 2014.
[Is] ISSN:2090-6803
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Acute leukemias are the most common childhood cancer in all age groups. Acute myeloid leukemias (AML) constitute about 15-20% of acute leukemias. Fatigability, pallor, fever, and bleeding are the most common presenting symptoms of AML. Hepatosplenomegaly and lymphadenopathy are commonly encountered during physical examination. In rare instances eruptions due to skin involvement and localized tumor masses (myeloid sarcoma) may be found. Myeloid sarcoma is especially seen in AML-M2 subtype. By cytogenetic analysis, in AML-M2 subtype t(8;21) is often seen and it is more probable to find inversion 16 in AML-M4Eos subtype. Herein, we present a 15-year-old girl whose initial symptom was abdominal pain for three days and her pathological sign was a large abdominal mass which was verified by imaging studies and diagnosed as myeloid sarcoma by biopsy. On bone marrow examination, she had diagnosis of AML-M2 and by cytogenetic analysis inversion 16 was positive. She was treated with AML-BFM 2004 protocol and she is being followed up in remission on her ninth month of the maintenance therapy.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Da] Date of entry for processing:150122
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1155/2014/246169

  6 / 316055 MEDLINE  
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[PMID]: 25610666
[Au] Autor:Koruyucu M; Barlak P; Seymen F
[Ad] Address:Faculty of Dentistry, Department of Pedodontics, Istanbul University, 34093 Istanbul, Turkey.
[Ti] Title:Oral and dental findings of dyskeratosis congenita.
[So] Source:Case Rep Dent;2014:454128, 2014.
[Is] ISSN:2090-6447
[Cp] Country of publication:Egypt
[La] Language:eng
[Ab] Abstract:Dyskeratosis congenital (DC) is a rare condition characterized by reticulate skin hyperpigmentation, mucosal leukoplakia, and nail dystrophy. More serious features are bone marrow involvement with pancytopenia and a predisposition to malignancy. The purpose of this case report is to describe the oral and dental findings in children with DC syndrome. A 10-year-old male diagnosed with DC was admitted because of extensive caries and toothache. Inadequate oral hygiene and extensive caries were observed in oral examination of the patient. Plaque accumulation was seen in gingival border of maxillary teeth. Papillary atrophy on the tongue was observed. Short and blunted roots of mandible incisors and upper and lower molars were determined on the radiographic examination. Dryness on the lips and commisuras, ectropion on his eyes, and epiphora were observed. Hematologic tests were performed and showed aplastic anemia at the age of 2. At the age of 4, the bone marrow transplantation was performed. Dermatological findings occurred after the bone marrow transplantation. The skin of the patient was thin, dry, and wrinkled in some areas. He had palmoplantar hyperkeratosis and syndactylia on his fingers. Endodontic treatment procedures were applied and other extensive caries are still being restored. The patient will be given full preventive care during regular follow-up. Oral hygiene was improved to the optimum level.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Da] Date of entry for processing:150122
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1155/2014/454128

  7 / 316055 MEDLINE  
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[PMID]: 25610653
[Au] Autor:Laupheimer M; Skorska A; Groe J; Tiedemann G; Steinhoff G; David R; Lux CA
[Ad] Address:Reference and Translation Center for Cardiac Stem Cell Therapy, University of Rostock, 18057 Rostock, Germany....
[Ti] Title:Selective Migration of Subpopulations of Bone Marrow Cells along an SDF-1α and ATP Gradient.
[So] Source:Bone Marrow Res;2014:182645, 2014.
[Is] ISSN:2090-2999
[Cp] Country of publication:Egypt
[La] Language:eng
[Ab] Abstract:Both stem cell chemokine stromal cell-derived factor-1α (SDF-1α) and extracellular nucleotides such as adenosine triphosphate (ATP) are increased in ischemic myocardium. Since ATP has been reported to influence cell migration, we analysed the migratory response of bone marrow cells towards a combination of SDF-1 and ATP. Total nucleated cells (BM-TNCs) were isolated from bone marrow of cardiac surgery patients. Migration assays were performed in vitro. Subsequently, migrated cells were subjected to multicolor flow cytometric analysis of CD133, CD34, CD117, CD184, CD309, and CD14 expression. BM-TNCs migrated significantly towards a combination of SDF-1 and ATP. The proportions of CD34+ cells as well as subpopulations coexpressing multiple stem cell markers were selectively enhanced after migration towards SDF-1 or SDF-1 + ATP. After spontaneous migration, significantly fewer stem cells and CD184+ cells were detected. Direct incubation with SDF-1 led to a reduction of CD184+ but not stem cell marker-positive cells, while incubation with ATP significantly increased CD14+ percentage. In summary, we found that while a combination of SDF-1 and ATP elicited strong migration of BM-TNCs in vitro, only SDF-1 was responsible for selective attraction of hematopoietic stem cells. Meanwhile, spontaneous migration of stem cells was lower compared to BM-TNCs or monocytes.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Da] Date of entry for processing:150122
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1155/2014/182645

  8 / 316055 MEDLINE  
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[PMID]: 25524774
[Au] Autor:Meiler S; Baumer Y; McCurdy S; Lee BH; Kitamoto S; Boisvert WA
[Ad] Address:From the Center for Cardiovascular Research, John A. Burns School of Medicine, University of Hawaii, Honolulu (S.M., Y.B., S.M., W.A.B.); Department of Food and Nutrition, School of Food Science and Technology, Chung-Ang University, Seoul, Korea (B.-H.L.); Departments of Cardiovascular Medicine and ...
[Ti] Title:Cluster of differentiation 43 deficiency in leukocytes leads to reduced atherosclerosis-brief report.
[So] Source:Arterioscler Thromb Vasc Biol;35(2):309-11, 2015 Feb.
[Is] ISSN:1524-4636
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:OBJECTIVE: The aim of this study was to investigate the role of cluster of differentiation 43 (CD43), an integral membrane glycoprotein with both proadhesive and antiadhesive activities, in atherosclerosis. APPROACH AND RESULTS: Low-density lipoprotein receptor-deficient mice were lethally irradiated and reconstituted with either bone marrow from CD43(-/-) mice or from wild-type controls. We found that mice lacking the CD43 on their leukocytes had significantly less severe atherosclerosis and that, contrary to our expectation, macrophage infiltration into the vessel wall was not affected by the lack of CD43 in the leukocytes. However, we found that CD43 mediates cholesterol homeostasis in macrophages by facilitating cholesterol efflux. This resulted in a significant reduction in storage of cholesterol in the aorta of mice lacking CD43 in the leukocytes. CONCLUSIONS: CD43 may be an important mediator of macrophage lipid homeostasis, thereby affecting macrophage foam cell formation and ultimately atherosclerotic plaque development.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1161/ATVBAHA.114.304513

  9 / 316055 MEDLINE  
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[PMID]: 25477345
[Au] Autor:Jiang H; Yazdanyar A; Lou B; Chen Y; Zhao X; Li R; Hoang Bui H; Kuo MS; Navab M; Qin S; Li Z; Jin W; Jiang XC
[Ad] Address:From the Department of Cell Biology, State University of New York, Downstate Medical Center, Brooklyn (H.J., A.Y., Y.C., X.Z., R.L., Z.L., W.J., X.C.J.); Fudan University, Shanghai, China (B.L., Y.C.); Molecular and Cellular Cardiology Program, VA New York Harbor Healthcare System, New York (Z.L., X...
[Ti] Title:Adipocyte phospholipid transfer protein and lipoprotein metabolism.
[So] Source:Arterioscler Thromb Vasc Biol;35(2):316-22, 2015 Feb.
[Is] ISSN:1524-4636
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:OBJECTIVE: Phospholipid transfer protein (PLTP) is highly expressed in adipose tissues. Thus, the effect of adipose tissue PLTP on plasma lipoprotein metabolism was examined. APPROACH AND RESULTS: We crossed PLTP-Flox-ΔNeo and adipocyte protein 2 (aP2)-Cre recombinase (Cre) transgenic mice to create PLTP-Flox-ΔNeo/aP2-Cre mice that have a 90 and a 60% reduction in PLTP mRNA in adipose tissue and macrophages, respectively. PLTP ablation resulted in a significant reduction in plasma PLTP activity (22%), high-density lipoprotein-cholesterol (21%), high-density lipoprotein-phospholipid (20%), and apolipoprotein A-I (33%) levels, but had no effect on nonhigh-density lipoprotein levels in comparison with those of PLTP-Flox-ΔNeo controls. To eliminate possible effects of PLTP ablation by macrophages, we lethally irradiated PLTP-Flox-ΔNeo/aP2-Cre mice and PLTP-Flox-ΔNeo mice, and then transplanted wild-type mouse bone marrow into them to create wild-type→PLTP-Flox-ΔNeo/aP2-Cre and wild-type→PLTP-Flox-ΔNeo mice. Thus, we constructed a mouse model (wild-type→PLTP-Flox-ΔNeo/aP2-Cre) with PLTP deficiency in adipocytes but not in macrophages. These knockout mice also showed significant decreases in plasma PLTP activity (19%) and cholesterol (18%), phospholipid (17%), and apolipoprotein A-I (26%) levels. To further investigate the mechanisms behind the reduction in plasma apolipoprotein A-I and high-density lipoprotein lipids, we measured apolipoprotein A-I-mediated cholesterol efflux in adipose tissue explants and found that endogenous and exogenous PLTP significantly increased cholesterol efflux from the explants. CONCLUSIONS: Adipocyte PLTP plays a small but significant role in plasma PLTP activity and promotes cholesterol efflux from adipose tissues.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1161/ATVBAHA.114.303764

  10 / 316055 MEDLINE  
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[PMID]: 25606057
[Au] Autor:Kim HG; Jang JH; Koh EH
[Ad] Address:Departments of Internal Medicine, Gyeongsang National University School of Medicine, 79 Gangnam-ro, Jinju, Korea.
[Ti] Title:TRIP11-PDGFRB fusion in a patient with a therapy-related myeloid neoplasm with t(5;14)(q33;q32) after treatment for acute promyelocytic leukemia.
[So] Source:Mol Cytogenet;7(1):103, 2014.
[Is] ISSN:1755-8166
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: Therapy-related myeloid neoplasm after treatment for acute promyelocytic leukemia (APL) is a relatively infrequent but severe complication. Most therapy-related myeloid neoplasms after treatment for APL are classified as therapy-related myelodysplastic syndrome or therapy-related acute myeloid leukemia. Translocation of 5q31-33, PDGFRB occur rarely in therapy-related myeloid neoplasm and there has been two identified PDGFRB partner genes located at 14q32, TRIP11 and KIAA1509. RESULTS: The TRIP11-PDGFRB fusion was identified in a patient with therapy-related myeloid neoplasm with t(5;14)(q33;q32) after treatment of APL using conventional cytogenetics, fluorescence in situ hybridization (FISH) and molecular methods. Cytogenetic analysis of the bone marrow aspirate revealed 46, XY, t(5;14)(q33;q32) in all 20 analyzed cells. No other cytogenetic abnormalities were observed. Break-apart FISH analysis demonstrated that rearrangement of PDGFRB at 5q33 was positive in 460 of 500 cells analyzed, while the PML-RARA rearrangement remained undetectable by RT-PCR. Sequencing of RT-PCR products revealed fusion between exon 16 of TRIP11 and exon 11 of PDGFRB. However, the KIAA1509-PDGFRB fusion was not detected by RT-PCR. CONCLUSION: We firstly demonstrated that therapy-related myeloid neoplasm with TRIP11-PDGFRB fusion was identified after treatment of APL.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1501
[Cu] Class update date: 150124
[Lr] Last revision date:150124
[Da] Date of entry for processing:150121
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1186/s13039-014-0103-6


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