Database : MEDLINE Search on : Caroli and Disease [Words] References found : 1147 [refine] Displaying: 1 .. 10   in format [Detailed]

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[PMID]: 23490563 [Au] Autor: Caroli A; Cardillo MT; Galea R; Biasucci LM [Ad] Address: Institute of Cardiology, Catholic University of the Sacred Heart, Rome, Italy. [Ti] Title: Potential therapeutic role of microRNAs in ischemic heart disease. [So] Source: J Cardiol;61(5):315-20, 2013 May. [Is] ISSN: 1876-4738 [Cp] Country of publication: Netherlands [La] Language: eng [Ab] Abstract: Cardiovascular disease (CVD) is the most important cause of death and illness in the western world. Atherosclerosis constitutes the single most important contributor to CVD. miRNAs are small ribonucleic acids (RNAs) that negatively regulate gene expression on the post-transcriptional level by inhibiting mRNA translation or promoting mRNA degradation. Several studies demonstrated that miRNAs dysregulation have a key role in the disease process and, focusing on atherosclerotic disease, in every step of plaque formation and destabilization. These data suggest a possible therapeutic application of miRNA modulation, in particular dysregulated miRNAs can be modulated in disease process antagonizing miRNAs up-regulated and increasing miRNAs down-regulated. In this review we summarize the miRNA therapeutic techniques (antimiR, mimics, sponges, masking, and erasers) underlining their therapeutic advantages and evaluating their risks and challenges. In particular, the use of miRNA modulators as a therapeutic approach opens a novel and fascinating area of intervention in the therapy of ischemic heart disease. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1305 [Js] Journal subset: IM [St] Status: In-Data-Review

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[PMID]: 23390179 [Au] Autor: Prestia A; Caroli A; van der Flier WM; Ossenkoppele R; Van Berckel B; Barkhof F; Teunissen CE; Wall AE; Carter SF; Schöll M; Choo IH; Nordberg A; Scheltens P; Frisoni GB [Ad] Address: LENITEM-Laboratory of Epidemiology Neuroimaging and Telemedicine, IRCCS Centro San Giovanni di Dio FBF, Brescia, Italy. [Ti] Title: Prediction of dementia in MCI patients based on core diagnostic markers for Alzheimer disease. [So] Source: Neurology;80(11):1048-56, 2013 Mar 12. [Is] ISSN: 1526-632X [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: OBJECTIVES: The current model of Alzheimer disease (AD) stipulates that brain amyloidosis biomarkers turn abnormal earliest, followed by cortical hypometabolism, and finally brain atrophy ones. The aim of this study is to provide clinical evidence of the model in patients with mild cognitive impairment (MCI). METHODS: A total of 73 patients with MCI from 3 European memory clinics were included. Brain amyloidosis was assessed by CSF Aß42 concentration, cortical metabolism by an index of temporoparietal hypometabolism on FDG-PET, and brain atrophy by automated hippocampal volume. Patients were divided into groups based on biomarker positivity: 1) Aß42- FDG-PET- Hippo-, 2) Aß42+ FDG-PET- Hippo-, 3) Aß42 + FDG-PET + Hippo-, 4) Aß42 + FDG-PET+ Hippo+, and 5) any other combination not in line with the model. Measures of validity were prevalence of group 5, increasing incidence of progression to dementia with increasing biological severity, and decreasing conversion time. RESULTS: When patients with MCI underwent clinical follow-up, 29 progressed to dementia, while 44 remained stable. A total of 26% of patients were in group 5. Incident dementia was increasing with greater biological severity in groups 1 to 5 from 4% to 27%, 64%, and 100% (p for trend < 0.0001), and occurred increasingly earlier (p for trend = 0.024). CONCLUSIONS: The core biomarker pattern is in line with the current pathophysiologic model of AD. Fully normal and fully abnormal pattern is associated with exceptional and universal development of dementia. Cases not in line might be due to atypical neurobiology or inaccurate thresholds for biomarker (ab)normality. [Mh] MeSH terms primary: Alzheimer Disease/diagnosis Alzheimer Disease/pathology Mild Cognitive Impairment/diagnosis Mild Cognitive Impairment/pathology [Mh] MeSH terms secundary: Aged Aged, 80 and over Alzheimer Disease/cerebrospinal fluid Amyloid beta-Peptides/cerebrospinal fluid Atrophy Biological Markers/cerebrospinal fluid Brain/pathology Dementia/diagnosis Dementia/pathology Disease Progression Female Follow-Up Studies Humans Male Middle Aged Mild Cognitive Impairment/cerebrospinal fluid Peptide Fragments/cerebrospinal fluid Predictive Value of Tests tau Proteins/cerebrospinal fluid [Pt] Publication type: JOURNAL ARTICLE; MULTICENTER STUDY; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Name of substance: 0 (Amyloid beta-Peptides); 0 (Biological Markers); 0 (Peptide Fragments); 0 (amyloid beta-protein (1-42)); 0 (tau Proteins) [Em] Entry month: 1304 [Js] Journal subset: AIM; IM [Da] Date of entry for processing: 130312 [St] Status: MEDLINE [do] DOI: 10.1212/WNL.0b013e3182872830

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[PMID]: 22518039 [Au] Autor: Cassanelli N; Caroli G; Dolci G; Dell'Amore A; Luciano G; Bini A; Stella F [Ad] Address: Department of Thoracic Surgery, Azienda Ospedaliero-Universitaria Sant'Orsola-Malpighi Hospital, Bologna, Italy. cassanelli.nicola@gmail.com [Ti] Title: Accuracy of transthoracic ultrasound for the detection of pleural adhesions. [So] Source: Eur J Cardiothorac Surg;42(5):813-8; discussion 818, 2012 Nov. [Is] ISSN: 1873-734X [Cp] Country of publication: Germany [La] Language: eng [Ab] Abstract: OBJECTIVES: In the era of minimally invasive surgery, preoperative detection of pleural adhesions can be very useful for the assessment of surgical approach, because pleural adhesions are the main contraindication to video-assisted thoracoscopy. The aim of this study was to assess the sensitivity and specificity of transthoracic ultrasound in the detection of pleural adhesions prior to thoracic surgery. METHODS: From February 2010 to January 2011, 142 consecutive patients (male, 98; female, 44; age range, 36-83 years, mean age, 63.4 years) undergoing surgical thoracic intervention (except for pneumothorax) were preoperatively scanned by two different surgeons. According to thoracic wall projections of lung segments, we created a nine-region topographic map, in which every pulmonary area was scanned to assess the presence or the absence of 'gliding sign' (lesion-by-lesion analysis). During operations the surgeon, blinded to the prediction, confirmed or excluded each suspected adhesion or documented other adhesions not previously identified. RESULTS: A total of 1192 predictions were made. Ultrasound predictions were confirmed 1124 times and refuted 68 times. Sensitivity was 80.6% (95% confidence interval, 0.740-0.872) and specificity 96.1% (95% confidence interval, 0.949-0.973). The positive predictive value was 73.2% and the negative predictive value was 97.4%. CONCLUSIONS: Transthoracic ultrasound is an effective method for predicting pleural adhesions before thoracic surgery in experienced hands. Its safety, feasibility and low cost make it a useful method for the planning of minimally invasive surgical interventions. [Mh] MeSH terms primary: Esophageal Neoplasms/surgery Lung Diseases/surgery Pleural Diseases/ultrasonography Preoperative Care/methods Surgical Procedures, Elective Thoracic Surgery, Video-Assisted/contraindications Thoracotomy [Mh] MeSH terms secundary: Adult Aged Aged, 80 and over Double-Blind Method Esophageal Neoplasms/complications Female Humans Lung Diseases/complications Male Middle Aged Pleural Diseases/complications Predictive Value of Tests Prospective Studies Sensitivity and Specificity Surgical Procedures, Elective/contraindications Tissue Adhesions/ultrasonography [Pt] Publication type: CLINICAL TRIAL; JOURNAL ARTICLE [Em] Entry month: 1304 [Js] Journal subset: IM [Da] Date of entry for processing: 121022 [St] Status: MEDLINE [do] DOI: 10.1093/ejcts/ezs144

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[PMID]: 23401780 [Au] Autor: Silvani A; Caroli M; Gaviani P; Fetoni V; Merli R; Riva M; De Rossi M; Imbesi F; Salmaggi A [Ad] Address: Fondazione I.R.C.C.S. Istituto Neurologico C. Besta, Milano, Italy. [Ti] Title: Neoplastic meningitis from solid tumors: a prospective clinical study in lombardia and a literature review on therapeutic approaches. [So] Source: J Drug Deliv;2013:147325, 2013. [Is] ISSN: 2090-3014 [Cp] Country of publication: Egypt [La] Language: eng [Ab] Abstract: Neoplastic dissemination to the leptomeninges is an increasingly common occurrence in patients with both haematological and solid tumors arising outside the central nervous system. Both refinement of diagnostic techniques (Magnetic resonance imaging) and increased survival in patients treated with targeted therapies for systemic tumors account for this increased frequency. Cerebrospinal fluid cytological analysis and MRI confirm clinical diagnosis based on multifocal central nervous system signs/symptoms in a patient with known malignancy. Overall survival in patients with leptomeningeal neoplastic dissemination from solid tumors is short, rarely exceeding 3-4 months. However, selected patients may benefit from aggressive therapies, Apart from symptomatic treatment, intrathecal chemotherapy is used, with both free (methotrexate, Thiotepa, AraC) and liposomal antitumor agents (liposomal AraC). Palliative radiotherapy is indicated only in cases of symptomatic bulky disease, surgery is limited to positioning of Ommaya recervoirs or C5F shunting. We report clinical data on a cohort of 26 prospectively followed patients with neoplastic leptomeningitis followed in Lombardia, Italy, in 2011. Prognostic factors and pattern of care are reported. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1302 [Cu] Class update date: 130418 [Lr] Last revision date: 130418 [Da] Date of entry for processing: 130212 [St] Status: PubMed-not-MEDLINE [do] DOI: 10.1155/2013/147325

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[PMID]: 23178392 [Au] Autor: Dell'Amore A; Monteverde M; Martucci N; Sanna S; Caroli G; Stella F; Dell'Amore D; Rocco G [Ad] Address: Thoracic Surgery Operative Unit, S. Orsola Malpighi University Hospital, University of Bologna, Bologna, Italy. [Ti] Title: Early and long-term results of pulmonary resection for non-small-cell lung cancer in patients over 75 years of age: a multi-institutional study. [So] Source: Interact Cardiovasc Thorac Surg;16(3):250-6, 2013 Mar. [Is] ISSN: 1569-9285 [Cp] Country of publication: England [La] Language: eng [Ab] Abstract: OBJECTIVES: Older lung cancer patients with multiple morbidities are increasingly referred to thoracic surgery departments. The aim of this multicenter study was to analyse the prognostic factors for in-hospital morbidity and mortality and to elucidate the predictors of long-term survival and oncological outcomes. METHODS: We identified 319 patients aged ≥ 75 years who underwent intended curative lung resection for lung cancer in three different thoracic surgery departments between January 2000 and December 2010. RESULTS: Seventy-one patients underwent limited resection, 202 had lobectomy, 16 had bilobectomy and 30 had pneumonectomy. The in-hospital mortality was 6.6%. Chronic renal failure, low respiratory reserve and pneumonectomy were predictors of in-hospital mortality. The mean follow-up time was 3.9 years, ranging from 1 month to 10.4 years. The disease-free survivals at 1, 3 and 5 years were 82, 60 and 47%, respectively. The overall survivals at 1, 3 and 5 years were 86, 59 and 38%, respectively. The long-term overall survival was negatively influenced by pneumonectomy, extended resection, N(1-2) subgroups and pathological TNM stage. CONCLUSIONS: Nowadays, we can consider surgery a safe and justifiable option for elderly patients. Careful preoperative work-up and selection are mandatory to gain satisfactory results. Good long-term results were achieved in elderly patients with early stage who underwent lobar or sublobar lung resection. The role of surgery or other alternative therapies, in patients with advanced stages, extensive nodal involvement and/or requiring extensive surgical resection for curative intent, is still unclear and further studies are certainly needed. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1302 [Js] Journal subset: IM [St] Status: In-Process [do] DOI: 10.1093/icvts/ivs473

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[PMID]: 22882197 [Au] Autor: Jarufe N; Figueroa E; Muñoz C; Moisan F; Varas J; Valbuena JR; Bambs C; Martínez J; Pimentel F [Ad] Address: Department of Digestive Surgery, Pontifical Catholic University of Chile, Santiago, Chile. njarufe@med.puc.cl [Ti] Title: Anatomic hepatectomy as a definitive treatment for hepatolithiasis: a cohort study. [So] Source: HPB (Oxford);14(9):604-10, 2012 Sep. [Is] ISSN: 1477-2574 [Cp] Country of publication: England [La] Language: eng [Ab] Abstract: BACKGROUND: Treatment requirements in hepatolithiasis may vary and may involve a multidisciplinary approach. Surgical resection has been proposed as a definitive treatment. OBJECTIVES: This study aimed to evaluate the clinical results of anatomic liver resection among Chilean patients with hepatolithiasis. METHODS: An historical cohort study was conducted. Patients who underwent hepatectomy as a definitive treatment for hepatolithiasis from January 1990 to December 2010 were included. Patients with a preoperative diagnosis of cholangiocarcinoma were excluded. Preoperative, operative and postoperative variables were evaluated. RESULTS: A total of 52 patients underwent hepatectomy for hepatolithiasis. The mean ± standard deviation patient age was 49.8 ± 11.8 years (range: 24-78 years); 65.4% of study subjects were female. A total of 75.0% of subjects had a history of previous cholecystectomy. The main presenting symptom was abdominal pain (82.7%). Hepatic involvement was noted in the left lobe in 57.7%, the right lobe in 34.6% and bilaterally in 7.7% of subjects. The rate of postoperative clearance of the biliary tree was 90.4%. Postoperative morbidity was 30.8% and there were no postoperative deaths. Three patients had recurrence of hepatolithiasis, which was associated with Caroli's disease in two of them. Overall 5-year survival was 94.5%. CONCLUSIONS: Anatomic liver resection is an effective treatment in selected patients with hepatolithiasis and is associated with low morbidity and no mortality. At longterm follow-up, anatomic hepatectomy in these patients was associated with a lower rate of recurrence. [Mh] MeSH terms primary: Bile Ducts, Intrahepatic/surgery Cholelithiasis/surgery Hepatectomy/methods [Mh] MeSH terms secundary: Adult Aged Biopsy Caroli Disease/complications Chi-Square Distribution Chile Cholecystectomy/adverse effects Cholelithiasis/diagnosis Cholelithiasis/etiology Cholelithiasis/mortality Cohort Studies Female Hepatectomy/adverse effects Hepatectomy/mortality Humans Kaplan-Meier Estimate Logistic Models Male Middle Aged Multivariate Analysis Postoperative Complications/etiology Recurrence Risk Assessment Risk Factors Time Factors Treatment Outcome Young Adult [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1212 [Cu] Class update date: 130416 [Lr] Last revision date: 130416 [Js] Journal subset: IM [Da] Date of entry for processing: 120813 [St] Status: MEDLINE [do] DOI: 10.1111/j.1477-2574.2012.00488.x

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[PMID]: 22884436 [Au] Autor: Dell'amore A; Nizar A; Dolci G; Cassanelli N; Caroli G; Luciano G; Greco D; Bini A; Stella F [Ad] Address: Thoracic Surgery Unit, S.Orsola-Malpighi Hospital, University of Bologna, Italy. dellamore76@libero.it [Ti] Title: Sternal resection and reconstruction for local recurrence of breast cancer using the sternal allograft transplantation technique. [So] Source: Heart Lung Circ;22(3):234-8, 2013 Mar. [Is] ISSN: 1444-2892 [Cp] Country of publication: Australia [La] Language: eng [Ab] Abstract: Sternal involvement in patients with breast cancer is relatively rare and its treatment is still controversial. Surgery is usually indicated in cases of single metastases in a multimodality protocol. Partial or complete sternectomy associated or not with the resection of surrounding tissues is the technique of choice to obtain safety margins and radical treatment of the disease. The most challenging part of the operation is the reconstruction of the anterior chest wall in order to avoid secondary complications and respiratory failure. In the last few years, different techniques and materials have been used to reconstruct the sternum. We report our experience in two patients with recurrent breast cancer using the sternal allograft technique to replace the sternum after partial sternectomy. The use of a sternal-allograft provides excellent functional and cosmetic results without complications during the follow-up period. The implantation technique is simple and reproducible. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1303 [Js] Journal subset: IM [St] Status: In-Process

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[PMID]: 22824346 [Au] Autor: Stella F; Dell'amore A; Nizar A; Cassanelli N; Caroli G; Luciano G; Greco D; Dolci G; Pirini G; Bini A [Ad] Address: Thoracic Surgery Operative Unit, S. Orsola-Malpighi University Hospital, Bologna, Italy. [Ti] Title: Desmoid tumour of the thoracic outlet in a 70 year-old man successfully removed through cervico-thoracic dartevelle approach. [So] Source: Heart Lung Circ;22(3):224-8, 2013 Mar. [Is] ISSN: 1444-2892 [Cp] Country of publication: Australia [La] Language: eng [Ab] Abstract: Desmoid tumours have a strong tendency for local invasion and recurrence. A 70 year-old male presented with cervical and left shoulder pain associated with a supraclavicular mass. The computed-tomography showed an expansive lesion measuring 10 cm × 6 cm × 5.5 cm in the left supraclavicular space. At magnetic resonance imaging the subclavian vessels and the brachial plexus were dislocated anteriorly but not infiltrated. An incisional biopsy suggested a desmoid tumour. An anterior cervicothoracic approach was used to remove the tumour. The chest wall was reconstructed with titanium bars and a polytetrafluoroethylene-patch. The clavicle was fixed using a titanium clip. The post-operative course was uneventful. The patient was treated with adjuvant radiation therapy. After six months the patient is in good clinical condition free from disease recurrence. In conclusion, desmoid tumour of the thoracic outlet is a challenging situation. Wide radical resection should be attempted whenever possible. The Dartevelle approach gives an optimal surgical field with direct control of vessels and nerve roots facilitating tumour dissection and radical resection en-bloc with the chest wall. The chest wall reconstruction with titanium bars and clips is a simple and effective method to guarantee good respiratory function and to stabilise the shoulder girdle. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1303 [Js] Journal subset: IM [St] Status: In-Process

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[PMID]: 23097114 [Au] Autor: Nakanuma Y; Sato Y; Harada K [Ad] Address: Department of Human Pathology, Kanazawa University Graduate School of Medicine, Kanazawa, Japan. pbcpsc@kenroku.kanazawa-u.ac.jp [Ti] Title: Tissue culture correlational study of genetic cholangiopathy of autosomal recessive polycystic kidney disease. [So] Source: Methods Mol Biol;945:303-18, 2013. [Is] ISSN: 1940-6029 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Cholangiocytes are epithelial cells that line the biliary tract and are also known as biliary epithelial cells (BECs). In vitro culture studies of BECs in correlation with tissue section examination may give us a comprehensive analysis of biliary tract diseases. Herein, we discuss genetic cholangiopathy of autosomal recessive polycystic kidney disease (ARPKD), mainly using a polycystic kidney (PCK) rat, an animal model of ARPKD. The hepatobiliary lesions in ARPKD patients (Caroli's disease and congenital hepatic fibrosis) and in PCK rats are speculated to be related to mutations to polycystic kidney and hepatic disease 1 (PKHD1) which have been recently demonstrated, though the exact causal relation between these mutations and hepatobiliary pathology remain to be clarified. Recently we clarified that BECs of PCK rat showed increased cell proliferation followed by irregular dilatation of intrahepatic bile ducts. We also identified the essential involvement of the MEK5-ERK5 pathway in the abnormal proliferation of BECs in the PCK rat. The degradation of laminin and type IV collagen (basal membrane components of bile ducts) was closely related to the biliary dysgenesis and cystogenesis in the PCK rats. BECs also showed mesenchymal phenotype followed by progressive portal tract fibrosis, indicating TGF-ß1 may be involved in this acquisition of mesenchymal phenotype. Detailed tissue culture correlation studies of ARPKD and PCK rats are mandatory to evaluate the pathogenesis of this genetic cholangiopathy. [Mh] MeSH terms primary: Polycystic Kidney, Autosomal Recessive/pathology Tissue Culture Techniques/methods [Mh] MeSH terms secundary: Animals Basement Membrane/drug effects Basement Membrane/metabolism Bile Ducts/pathology Blotting, Western Cell Culture Techniques Cell Proliferation/drug effects Collagen Type IV/metabolism Epithelial Cells/drug effects Epithelial Cells/metabolism Epithelial Cells/pathology Fibrinolysin/antagonists & inhibitors Humans Matrix Metalloproteinases/genetics Matrix Metalloproteinases/metabolism Phenotype Polycystic Kidney, Autosomal Recessive/metabolism Quinazolines/pharmacology Rats Transforming Growth Factor beta1/pharmacology [Pt] Publication type: JOURNAL ARTICLE [Nm] Name of substance: 0 (Collagen Type IV); 0 (Quinazolines); 0 (Transforming Growth Factor beta1); 184475-35-2 (gefitinib); EC 3.4.21.7 (Fibrinolysin); EC 3.4.24.- (Matrix Metalloproteinases) [Em] Entry month: 1303 [Js] Journal subset: IM [Da] Date of entry for processing: 121025 [St] Status: MEDLINE [do] DOI: 10.1007/978-1-62703-125-7_18

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[PMID]: 22365486 [Au] Autor: Morbelli S; Drzezga A; Perneczky R; Frisoni GB; Caroli A; van Berckel BN; Ossenkoppele R; Guedj E; Didic M; Brugnolo A; Sambuceti G; Pagani M; Salmon E; Nobili F [Ad] Address: Nuclear Medicine Unit, Department of Internal Medicine, San Martino University Hospital, Genoa, Italy. silviadaniela.morbelli@hsanmartino.it [Ti] Title: Resting metabolic connectivity in prodromal Alzheimer's disease. A European Alzheimer Disease Consortium (EADC) project. [So] Source: Neurobiol Aging;33(11):2533-50, 2012 Nov. [Is] ISSN: 1558-1497 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: We explored resting-state metabolic connectivity in prodromal Alzheimer's disease (pAD) patients and in healthy controls (CTR), through a voxel-wise interregional correlation analysis of 18F-fluorodeoxyglucose (FDG)-positron emission tomography (PET) by means of statistical parametric mapping. Baseline 18F-fluorodeoxyglucose-positron emission tomography of 36 patients with amnestic mild cognitive impairment who converted to Alzheimer's disease (AD) dementia after an average time of 2 years (pAD) and of 105 CTR were processed. The area of hypometabolism in pAD showed less metabolic connectivity in patients than in CTR (autocorrelation and correlation with large temporal and frontal areas, respectively). pAD patients showed limited correlation even in selected nonhypometabolic areas, including the hippocampi and the dorsolateral prefrontal cortex (DLFC). On the contrary, in CTR group correlation was highlighted between hippocampi and precuneus/posterior cingulate and frontal cortex, and between dorsolateral prefrontal cortex and caudate nuclei and parietal cortex. The reduced metabolic connections both in hypometabolic and nonhypometabolic areas in pAD patients suggest that metabolic disconnection (reflecting early diaschisis) may antedate remote hypometabolism (early sign of synaptic degeneration). [Mh] MeSH terms primary: Alzheimer Disease/metabolism Cognition Disorders/metabolism [Mh] MeSH terms secundary: Aged Alzheimer Disease/diagnosis Alzheimer Disease/radionuclide imaging Brain Mapping Cognition Disorders/diagnosis Female Fluorodeoxyglucose F18/diagnostic use Follow-Up Studies Hippocampus/metabolism Humans Image Interpretation, Computer-Assisted/methods Male Middle Aged Positron-Emission Tomography/methods Rest [Pt] Publication type: JOURNAL ARTICLE [Nm] Name of substance: 63503-12-8 (Fluorodeoxyglucose F18) [Em] Entry month: 1303 [Js] Journal subset: IM [Da] Date of entry for processing: 120903 [St] Status: MEDLINE [do] DOI: 10.1016/j.neurobiolaging.2012.01.005

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