Database : MEDLINE
Search on : Ciliophora and Infections [Words]
References found : 465 [refine]
Displaying: 1 .. 10   in format [Detailed]

page 1 of 47 go to page                         

  1 / 465 MEDLINE  
              next record last record
select
to print
Photocopy
Full text

[PMID]: 29256279
[Au] Autor:Herczeg D; Sipos D; Dán Á; Loy C; Kallert DM; Eszterbauer E
[Ad] Address:1 Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences , Hungária krt. 21, H-1143 Budapest , Hungary.
[Ti] Title:The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus carpio) to the white spot parasite, Ichthyophthirius multifiliis.
[So] Source:Acta Vet Hung;65(4):517-530, 2017 12.
[Is] ISSN:0236-6290
[Cp] Country of publication:Hungary
[La] Language:eng
[Ab] Abstract:One of the main obstacles in freshwater aquaculture is the parasitic ciliate Ichthyophthirius multifiliis (Ich), the causative agent of white spot disease. The use of immunostimulants as feed additives may be a promising approach to control Ich infection. In the present study, we tested the prophylactic effect of orally administered ß-1,3/1,6-glucan and propolis extract E50 against Ich infection in common carp. In total, 122 fish were separated into three experimental groups fed with a control, 3% ß-glucan and 1% propolis diet for 40 consecutive days, respectively. On day 40, 16 fish per group were individually exposed to Ich theronts and the number of trophonts was counted 5 days post exposure. Relative gene expression of interleukin 1-ß (IL-1-ß) in common carp liver was examined by qPCR. Compared to control, the mean infection intensity was lower in the ß-glucan- and propolis-fed groups; however, the difference was not statistically significant. The relative expression of IL-1-ß significantly decreased in the propolis-fed group at day 10. In the ß-glucan-fed group, a significant IL-1-ß decrease was detected at day 15 compared to control. Although the Ich infection intensity was slightly decreased in both treated groups, and IL-1-ß was moderately down-regulated in the liver of common carp, our results suggest that the applied feeding regime is insufficient to prevent Ich outbreaks in common carp.
[Mh] MeSH terms primary: Adjuvants, Immunologic/administration & dosage
Carps
Ciliophora Infections/veterinary
Ciliophora
Dietary Supplements
Fish Diseases/parasitology
[Mh] MeSH terms secundary: Animals
Ciliophora/genetics
Ciliophora/isolation & purification
Ciliophora Infections/parasitology
Ciliophora Infections/prevention & control
RNA/genetics
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Adjuvants, Immunologic); 63231-63-0 (RNA)
[Em] Entry month:1803
[Cu] Class update date: 180305
[Lr] Last revision date:180305
[Js] Journal subset:IM
[Da] Date of entry for processing:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.050

  2 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28528516
[Au] Autor:Fu YW; Wang B; Zhang QZ; Xu DH; Lin DJ; Yang XY; Zhu SQ; Pan JY; Deng Q; Liu YM; Zhou SY
[Ad] Address:Institute of Hydrobiology, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Key Laboratory of Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, People's Republic of China.
[Ti] Title:Combined effects of Chinese medicine feed and ginger extract bath on co-infection of Ichthyophthirius multifiliis and Dactylogyrus ctenopharyngodonid in grass carp.
[So] Source:Parasitol Res;116(7):2017-2025, 2017 Jul.
[Is] ISSN:1432-1955
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Dactylogyrus ctenopharyngodonid and Ichthyophthirius multifiliis are two important ectoparasites of freshwater fish. Co-infection by the two parasites leads to high fish mortality and results in heavy economic losses. This study aimed to evaluate the efficacy of medicated feed and a ginger extract bath against D. ctenopharyngodonid and I. multifiliis on grass carp and investigate the hematological response of grass carp co-infected by the two parasites. These results demonstrated that red blood cell (RBC) and thrombocyte percentage among leucocytes significantly decreased after grass carp were co-infected by D. ctenopharyngodonid and I. multifiliis. The monocyte and neutrophil percentages significantly increased with the increment of parasite mean intensities, while the lymphocyte percentage decreased. The activities of serum acid phosphatase (ACP), alkaline phosphatase (AKP), lysozyme (LZM), and superoxide dismutase (SOD) significantly increased after co-infection. When grass carp treated with medicated feed containing 4% of Astragalus membranaceus, Allium sativum, Morus alba, and Glycyrrhiza uralensis, the activities of ACP, AKP, LZM, and SOD were significantly enhanced, and the mean intensities of D. ctenopharyngodonid and I. multifiliis were significantly decreased. When grass carp was treated with medicated feed and a 4-mg/L ginger extract bath, all parasites were eliminated during 28 days. The bath of ginger extract at a concentration of 4 mg/L kept a low mean intensity of I. multifiliis and D. ctenopharyngodonid, then the two parasites were eliminated by oral administration of the medicated feed with an immunostimulant (Chinese medicine compound).
[Mh] MeSH terms primary: Carps/parasitology
Ciliophora Infections/veterinary
Drugs, Chinese Herbal/therapeutic use
Fish Diseases/parasitology
Hymenostomatida
Trematode Infections/veterinary
[Mh] MeSH terms secundary: Animal Feed
Animals
Ciliophora Infections/drug therapy
Coinfection
Garlic
Ginger
Hymenostomatida/drug effects
Trematoda
Trematode Infections/drug therapy
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Drugs, Chinese Herbal)
[Em] Entry month:1711
[Cu] Class update date: 171101
[Lr] Last revision date:171101
[Js] Journal subset:IM
[Da] Date of entry for processing:170522
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5507-x

  3 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28443822
[Au] Autor:Kvapil P; Kastelic M; Dovc A; Bartova E; Cizek P; Lima N; Strus S
[Ad] Address:Zoo Ljubljana, Ljubljana, Slovenia.
[Ti] Title:An eight-year survey of the intestinal parasites of carnivores, hoofed mammals, primates, ratites and reptiles in the Ljubljana zoo in Slovenia.
[So] Source:Folia Parasitol (Praha);64, 2017 Apr 21.
[Is] ISSN:0015-5683
[Cp] Country of publication:Czech Republic
[La] Language:eng
[Ab] Abstract:Problems with parasitic infections and their interspecies transmissions are common in zoological gardens and could pose serious health damage to captive animals. This study presents results of eight-year monitoring of intestinal parasites in animals from Zoo Ljubljana, Slovenia. A total of 741 faecal samples from 40 animal species were collected two to four times per year and examined microscopically. Intestinal parasites were detected in 45% of samples, with detection of helminths (Cestoda, Nematoda - Ascaridida, Enoplida, Strongylida, Oxyurida, Rhabditida and Trichurida) and protists (Apicomplexa and Ciliophora) in 25% and 13% of samples, respectively; mixed infection was found in 7% of samples. The mostly infected were ungulates (61%), followed by reptiles (44%), ratites (29%), primates (22%) and carnivores (7%). During the observation period, the number of infected animal species increased from 8 to 25. This is the first long-term monitoring study of intestinal parasites in zoo animals from Slovenia. Routine monitoring of parasitic infection and regular deworming and hygienic measures are necessary to prevent gastrointestinal infections in captive animals.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1704
[Cu] Class update date: 170426
[Lr] Last revision date:170426
[St] Status:In-Data-Review

  4 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28428147
[Au] Autor:Chi H; Taik P; Foley EJ; Racicot AC; Gray HM; Guzzetta KE; Lin HY; Song YL; Tung CH; Zenke K; Yoshinaga T; Cheng CY; Chang WJ; Gong H
[Ad] Address:Biotechnology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China.
[Ti] Title:High genetic diversities between isolates of the fish parasite Cryptocaryon irritans (Ciliophora) suggest multiple cryptic species.
[So] Source:Mol Phylogenet Evol;112:47-52, 2017 Jul.
[Is] ISSN:1095-9513
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The ciliate protozoan Cryptocaryon irritans parasitizes marine fish and causes lethal white spot disease. Sporadic infections as well as large-scale outbreaks have been reported globally and the parasite's broad host range poses particular threat to the aquaculture and ornamental fish markets. In order to better understand C. irritans' population structure, we sequenced and compared mitochondrial cox-1, SSU rRNA, and ITS-1 sequences from 8 new isolates of C. irritans collected in China, Japan, and Taiwan. We detected two SSU rRNA haplotypes, which differ at three positions, separating the isolates into two main groups (I and II). Cox-1 sequences also support the division into two groups, and the cox-1 divergence between these two groups is unexpectedly high (9.28% for 1582 nucleotide positions). The divergence is much greater than that detected in Ichthyophthirius multifiliis, the ciliate protozoan causing freshwater white spot disease in fish, where intraspecies divergence on cox-1 sequence is only 1.95%. ITS-1 sequences derived from these eight isolates and from all other C. irritans isolates (deposited in the GenBank) not only support the two groups, but further suggest the presence of a third group with even greater sequence divergence. Finally, a small Ka/Ks ratio estimated from cox-1 sequences suggests that this gene in C. irritans remains under strong purifying selection. Taken together, the C. irritans species may consists of many subspecies and/or syngens. Further work is needed to determine if there is reproductive isolation between the groups we have defined.
[Mh] MeSH terms primary: Genetic Variation
Hymenostomatida/genetics
[Mh] MeSH terms secundary: Animals
Aquaculture
China
Fishes/parasitology
Genetic Speciation
Japan
Phylogeny
Taiwan
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1710
[Cu] Class update date: 171018
[Lr] Last revision date:171018
[Js] Journal subset:IM
[Da] Date of entry for processing:170422
[St] Status:MEDLINE

  5 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28291600
[Au] Autor:Bastos Gomes G; Miller TL; Vaughan DB; Jerry DR; McCowan C; Bradley TL; Hutson KS
[Ad] Address:Marine and Aquaculture Sciences, College of Science and Engineering and Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, 4811, QLD, Australia. Electronic address: giana.bastosgomes@my.jcu.edu.au.
[Ti] Title:Evidence of multiple species of Chilodonella (Protozoa, Ciliophora) infecting Australian farmed freshwater fishes.
[So] Source:Vet Parasitol;237:8-16, 2017 Apr 15.
[Is] ISSN:1873-2550
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Parasitic Chilodonella species, Chilodonella piscicola and Chilodonella hexasticha, cause considerable economic losses globally to freshwater farmed fish production. Some genetic studies of Chilodonella spp. have indicated that many species within the genus may form cryptic species complexes. To understand the diversity of Chilodonella spp. infecting Australian freshwater farmed fish, specimens were isolated from infected barramundi (Lates calcarifer) and Murray cod (Maccullochella peelii) from fish farms in tropical north Queensland (QLD), temperate Victoria (Vic) and New South Wales (NSW) for genetic and morphological analysis. Parasites were stained and measured for morphological description and comparative phylogenetic analyses were performed using the mitochondrial small subunit (mtSSU) rDNA marker. Morphological analyses revealed four distinct morphotypes of Chilodonella infecting farmed barramundi and Murray Cod. Three putative species were isolated from barramundi (Chilodonella hexasticha, C. acuta and C. uncinata) and one from Murray cod (C. piscicola). However, phylogenetic analyses detected only three distinct genotypes, with the putative C. hexasticha and C. piscicola sharing 100% sequence identity. This suggests that Australian isolates of C. hexasticha and C. piscicola could represent the same species and may exhibit phenotypic plasticity. Further molecular analysis, including isolates from the type localities, should be performed to support or refute the synonymy of these species.
[Mh] MeSH terms primary: Ciliophora Infections/veterinary
Fish Diseases/parasitology
Hymenostomatida/classification
[Mh] MeSH terms secundary: Animals
Ciliophora Infections/parasitology
Coinfection/veterinary
DNA, Mitochondrial/chemistry
DNA, Mitochondrial/genetics
DNA, Protozoan/chemistry
DNA, Protozoan/genetics
DNA, Ribosomal/chemistry
DNA, Ribosomal/genetics
Hymenostomatida/genetics
Hymenostomatida/isolation & purification
Hymenostomatida/ultrastructure
New South Wales
Perciformes
Phylogeny
Queensland
Victoria
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Mitochondrial); 0 (DNA, Protozoan); 0 (DNA, Ribosomal)
[Em] Entry month:1709
[Cu] Class update date: 170926
[Lr] Last revision date:170926
[Js] Journal subset:IM
[Da] Date of entry for processing:170315
[St] Status:MEDLINE

  6 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28288756
[Au] Autor:Liu YM; Zhang QZ; Xu DH; Fu YW; Lin DJ; Zhou SY; Li JP
[Ad] Address:Key Laboratory of Aquatic Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Institute of Hydrobiology, Jinan University, West 601 Huangpu Avenue, Tianhe Distri
[Ti] Title:Antiparasitic efficacy of curcumin from Curcuma longa against Ichthyophthirius multifiliis in grass carp.
[So] Source:Vet Parasitol;236:128-136, 2017 Mar 15.
[Is] ISSN:1873-2550
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Ichthyophthirius multifiliis is a ciliated parasite that elicits great economic losses in aquaculture. In the present study, a polyphenol compound, curcumin, was obtained from the rhizome of Curcuma longa by bioassay-guided isolation based on the efficacy of anti-I. multifiliis theronts. Anti-I. multifiliis efficacy of curcumin was evaluated in vitro and in vivo. Curcumin resulted in 100% mortality of I. multifiliis theronts at a concentration of 1mg/L within 21.7±1.2min and killed all tomonts at 8mg/L within 31.0±1.0min. Curcumin at 4mg/L for 16h exposure can completely terminate the reproduction of tomonts. The pretreatment with curcumin at concentrations of 0.5, 0.25, and 0.125mg/L for 2h significantly reduced the infectivity of I. multifiliis theronts. Curcumin at 4mg/L completely cured the infected grass carp and protected naive fish from I. multifiliis infection after 10days exposure. The 4h median effective concentration (EC ) of curcumin to I. multifiliis theronts and the 5h EC of curcumin to I. multifiliis tomonts were 0.303mg/L and 2.891mg/L, respectively. The 96h median lethal concentration (LC ) of curcumin to grass carp was 56.8mg/L, which was approximately 187.4 times EC of curcumin to theronts and 19.6 times EC of curcumin to tomonts. The results demonstrated that curcumin has the potential to be a safe and effective therapeutant for controlling ichthyophthiriasis in aquaculture.
[Mh] MeSH terms primary: Antiprotozoal Agents/pharmacology
Carps
Ciliophora Infections/veterinary
Curcumin/pharmacology
Fish Diseases/drug therapy
Hymenostomatida/drug effects
[Mh] MeSH terms secundary: Animals
Antiprotozoal Agents/adverse effects
Ciliophora Infections/drug therapy
Ciliophora Infections/parasitology
Curcumin/adverse effects
Dose-Response Relationship, Drug
Fish Diseases/parasitology
Hymenostomatida/physiology
Random Allocation
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antiprotozoal Agents); IT942ZTH98 (Curcumin)
[Em] Entry month:1709
[Cu] Class update date: 170908
[Lr] Last revision date:170908
[Js] Journal subset:IM
[Da] Date of entry for processing:170315
[St] Status:MEDLINE

  7 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28286257
[Au] Autor:Li ZX; Li YW; Xu S; Xu Y; Mo ZQ; Dan XM; Luo XC
[Ad] Address:School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China.
[Ti] Title:Grouper (Epinephelus coioides) TCR signaling pathway was involved in response against Cryptocaryon irritans infection.
[So] Source:Fish Shellfish Immunol;64:176-184, 2017 May.
[Is] ISSN:1095-9947
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:T cell activation is a complicated process accompanying with the activation of T cell receptor (TCR) signaling pathway, which is not well described in teleost fish. The initiation of this pathway depends on the interaction of membrane TCR co-receptors (e.g. CD4/8, CD3 and CD45) and a series of cytoplasmic protein tyrosine kinases (e.g. Lck, Fyn and ZAP70). Cyptocaryon irritans is a ciliate pathogen of marine fish white spot disease causing huge economic lost in marine aquaculture. This parasite can infect fish gill and skin and is considered to be a good pathogen model for fish gill and skin mucosal immunity. Our previous studies showed the locally mucosal antibody response was important for fish defense against this parasite. While how TCR signaling pathway involved in T cell activation to help B cell activation in C. irritans infected fish is still not known. In the present study, we cloned a grouper TCR co-receptor gene EcCD3ε (537 bp) and its three kinase genes, including EcLck (1512 bp), EcFyn (1605 bp) and EcZAP70 (1893 bp). Homology analysis showed that they all shared the highest identity with corresponding genes from Takifugu rubripes (EcCD3ε 41%, EcLck 88%, EcFyn 98% and EcZAP70 93%), and their conserved motifs involved in the signaling transduction were analyzed. The tissue distribution analysis showed these four genes were high expressed in thymus, and it is interesting to find their comparative high expression in skin, gill and midgut mucosal immune tissues. In C. irritans infected grouper, the expression of three TCR co-receptors (EcCD4-1, EcCD3ε and EcCD45) and three kinases (EcLck, EcFyn and EcZAP70) was tested in skin, gill, head kidney and spleen at 0, 12 h, 24 h, 2 d, 3 d, 5 d and 7 d. All six genes were significantly up-regulated in skin at most tested time points, which indicate the possibility of skin local T cell activation to support the local antibody response. Compared to three TCR co-receptors, significantly up-regulation of three kinases were seen in the spleen, and the spleen fold changes of these three kinases were much higher than head kidney, which indicates spleen maybe the major systematic immune organs for T cell activation in C. irritans infected fish.
[Mh] MeSH terms primary: Bass
Ciliophora Infections/veterinary
Fish Diseases/genetics
Fish Proteins/genetics
Receptors, Antigen, T-Cell/genetics
Signal Transduction
[Mh] MeSH terms secundary: Animals
Ciliophora/physiology
Ciliophora Infections/genetics
Ciliophora Infections/immunology
Ciliophora Infections/parasitology
Cloning, Molecular
DNA, Complementary/genetics
DNA, Complementary/metabolism
Fish Diseases/immunology
Fish Diseases/parasitology
Fish Proteins/metabolism
Immunity, Mucosal
Phylogeny
RNA, Messenger/genetics
RNA, Messenger/metabolism
Receptors, Antigen, T-Cell/metabolism
Sequence Analysis, DNA/veterinary
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Complementary); 0 (Fish Proteins); 0 (RNA, Messenger); 0 (Receptors, Antigen, T-Cell)
[Em] Entry month:1706
[Cu] Class update date: 170629
[Lr] Last revision date:170629
[Js] Journal subset:IM
[Da] Date of entry for processing:170314
[St] Status:MEDLINE

  8 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28159696
[Au] Autor:Bai JS; Li YW; Deng Y; Huang YQ; He SH; Dai J; Zhao SZ; Dan XM; Luo XC
[Ad] Address:Guangzhou Airport Extry-Exit Inspection and Quarantine Bureau, Guangzhou 510470, Guangdong Province, PR China. Electronic address: bjslinyi@qq.com.
[Ti] Title:Molecular identification and expression analysis of TLR5M and TLR5S from orange-spotted grouper (Epinepheluscoioides).
[So] Source:Fish Shellfish Immunol;63:97-102, 2017 Apr.
[Is] ISSN:1095-9947
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Toll-like receptor 5 (TLR5) is an important receptor that interacts with bacterial flagellin and regulates host immune response in mammal. Recent studies demonstrate that piscine contains two types of TLR5, namely membrane form of TLR5 (TLR5M) and soluble form of TLR5 (TLR5S), and both of which perform crucial role in flagellin response. In the present study, a TLR5M and a TLR5S sequence was cloned from orange-spotted grouper (Epinepheluscoioides), and their ORFs are respectively 2466 bp (821 aas) and 1935 bp (644 aas). EcTLR5M has the typical TLR structure of a LRR domain, a transmembrane region and a TIR domain, while EcTLR5S only contains a LRR domain like other species' TLR5S. Both molecules have 23 LRR motifs, a LRR-NT and a LRR-CT in the LRR domain, similar to those of other species. Phylogenetic and sequence alignment indicated that both EcTLR5s respectively displayed closer relationship and higher sequence identity with those in other fish species. In healthy grouper, EcTLR5M was highly expressed in the skin, head kidney and spleen, while EcTLR5S was mainly detected in the liver. Ciliate Cryptocaryon irritans infection could significantly up-regulate the expression level of EcTLR5s in the gill and spleen from day 1 to day 3, and higher expression fold change was observed in the spleen. Taken together, the present studies contributed to understanding the function of piscine TLR5M/S and clarify their possible role in fish immune response against ciliate infection.
[Mh] MeSH terms primary: Bass/genetics
Bass/immunology
Fish Proteins/genetics
Gene Expression Regulation
Toll-Like Receptor 5/genetics
[Mh] MeSH terms secundary: Animals
Bass/classification
Ciliophora/immunology
Ciliophora Infections/immunology
Ciliophora Infections/parasitology
Ciliophora Infections/veterinary
DNA, Complementary/genetics
DNA, Complementary/metabolism
Expressed Sequence Tags
Fish Diseases/immunology
Fish Diseases/parasitology
Fish Proteins/metabolism
Gene Expression Profiling
Immunity, Innate/genetics
Phylogeny
RNA, Messenger/genetics
RNA, Messenger/metabolism
Sequence Analysis, DNA/veterinary
Toll-Like Receptor 5/metabolism
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Complementary); 0 (Fish Proteins); 0 (RNA, Messenger); 0 (Toll-Like Receptor 5)
[Em] Entry month:1705
[Cu] Class update date: 170503
[Lr] Last revision date:170503
[Js] Journal subset:IM
[Da] Date of entry for processing:170205
[St] Status:MEDLINE

  9 / 465 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 27864673
[Au] Autor:Yao JY; Xu Y; Yuan XM; Yin WL; Yang GL; Lin LY; Pan XY; Wang CF; Shen JY
[Ad] Address:Agriculture Ministry Key Laboratory of Healthy Freshwater Aquaculture, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou, 313001, China.
[Ti] Title:Proteomic analysis of differentially expressed proteins in the two developmental stages of Ichthyophthirius multifiliis.
[So] Source:Parasitol Res;116(2):637-646, 2017 Feb.
[Is] ISSN:1432-1955
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Ichthyophthirius is a severe disease of farmed freshwater fish caused by the parasitic ciliate Ichthyophthirius multifiliis (Ich). This disease can lead to considerable economic loss, but the protein profiles in different developmental stages of the parasite remain unknown. In the present study, proteins from trophonts and theronts of Ich were identified by isobaric tags for relative and absolute quantitation (iTRAQ). A total of 2300 proteins were identified in the two developmental stages, of which 1520 proteins were differentially expressed. Among them, 84 proteins were uniquely expressed in the theronts stage, while 656 proteins were expressed only in trophonts. The differentially expressed proteins were catalogued (assorted) to various functions of Ich life cycle, including biological process, cellular component, and molecular function that occur at distinct stages. Using a 1.5-fold change in expression as a physiologically significant benchmark, a lot of differentially expressed proteins were reliably quantified by iTRAQ analysis. Two hundred forty upregulated and 57 downregulated proteins in the trophonts stage were identified as compared with theronts. The identified proteins were involved in various functions of the I. multifiliis life cycle, including binding, catalytic activity, structural molecule activity, and transporter activity. Further investigation of the transcriptional levels of periplasmic immunogenic protein, transketolase, zinc finger, isocitrate dehydrogenase, etc., from the different protein profiles using quantitative RT-PCR showed identical results to the iTRAQ analysis. This work provides an effective resource to further our understanding of Ich biology, and lays the groundwork for the identification of potential drug targets and vaccines candidates for the control of this devastating fish pathogen.
[Mh] MeSH terms primary: Hymenostomatida/growth & development
Hymenostomatida/metabolism
Proteomics/methods
[Mh] MeSH terms secundary: Animals
Carps/parasitology
Ciliophora Infections/parasitology
Fish Diseases/parasitology
Gene Expression Regulation
Life Cycle Stages
Tandem Mass Spectrometry/methods
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1705
[Cu] Class update date: 171007
[Lr] Last revision date:171007
[Js] Journal subset:IM
[Da] Date of entry for processing:161120
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-016-5328-3

  10 / 465 MEDLINE  
              first record previous record
select
to print
Photocopy
Full text

[PMID]: 27801984
[Au] Autor:Moreira GS; Shoemaker CA; Zhang D; Xu DH
[Ad] Address:Laboratory of Parasitology, College of Animal Science and Food Engineering, University of São Paulo (USP), Pirassununga, Brazil.
[Ti] Title:Expression of immune genes in skin of channel catfish immunized with live theronts of Ichthyophthirius multifiliis.
[So] Source:Parasite Immunol;39(1), 2017 Jan.
[Is] ISSN:1365-3024
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:The objective of this study was to evaluate differential expression of innate and adaptive immune genes, including immunoglobulin, immune cell receptor, cytokine, inflammatory protein, toll-like receptors (TLR) and recombination-activating gene (RAG) in skin from channel catfish, Ictalurus punctatus after immunization with live theronts of Ichthyophthirius multifiliis (Ich) by intraperitoneal injection. The immunized catfish showed significantly higher survival rate (95%) than those of mock-immunized control fish (0% survival) after the theront challenge. The gene expression of innate immune system, such as cytokines (IL-1ß type a, IL-1ß type b, IFN-γ, TGF1-ß and TNF-α) and inflammatory proteins (NF-kB and iNOS 2), showed significant upregulation at day 1 (D1) post-immunization. Expression of TLR genes exhibited a rapid increase from hour 4 (h4) to D10 post-immunization. Genes of the adaptive response, such as the cell receptor MHC I, CD8 , CD4 and TCR-α, showed upregulation at D1, D6 and D10. The TCR-ß expression increased rapidly at h4 and remained upregulated until D10. Immunoglobulin IgM upregulation was detected from h4 until D2 while IgD expression was increased from D1 until D10. Rapid upregulation of innate and adaptive immune genes in skin of catfish following live theront vaccination was demonstrated in this study ultimately resulting in significant protection against Ich infection.
[Mh] MeSH terms primary: Ciliophora Infections/veterinary
Fish Diseases/immunology
Hymenostomatida/immunology
Ictaluridae/immunology
Ictaluridae/parasitology
Skin/immunology
[Mh] MeSH terms secundary: Animals
Antibodies, Protozoan/immunology
Ciliophora Infections/immunology
Fish Diseases/parasitology
Immunization/veterinary
Immunoglobulin M
NF-kappa B
Tumor Necrosis Factor-alpha
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antibodies, Protozoan); 0 (Immunoglobulin M); 0 (NF-kappa B); 0 (Tumor Necrosis Factor-alpha)
[Em] Entry month:1709
[Cu] Class update date: 170912
[Lr] Last revision date:170912
[Js] Journal subset:IM
[Da] Date of entry for processing:161102
[St] Status:MEDLINE
[do] DOI:10.1111/pim.12397


page 1 of 47 go to page                         
   


Refine the search
  Database : MEDLINE Advanced form   

    Search in field  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/PAHO/WHO - Latin American and Caribbean Center on Health Sciences Information