Database : MEDLINE
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[PMID]: 29516044
[Au] Autor:Stanila DM; Florea AM; Stanila A; Panga AA
[Ad] Address:Department of Ophthalmology, Clinical Emergency Hospital Sibiu, Sibiu, Romania.
[Ti] Title:Endothelial cells loss to the hyperopic pacients during phacoemulsification.
[So] Source:Rom J Ophthalmol;61(4):256-260, 2017 Oct-Dec.
[Is] ISSN:2457-4325
[Cp] Country of publication:Romania
[La] Language:eng
[Ab] Abstract:Introduction: The phacoemulsification cataract surgery is the most frequently performed surgery and it generally improves vision in over 90% of the patients. Hyperopic patients are a challenge during phacoemulsification especially because of their short eyeball and shallow anterior chamber. A shallow anterior chamber is associated with overall reduction of the safe zone, which may lead to difficulty in creating the corneal incisions, harder capsulorhexis performing, or endothelial complications. Purpose: The aim of the study was to present the endothelial cells loss after the phacoemulsification procedure in the hyperopic patients. Material and Methods: A number of 1775 patients operated in the Ophthalmology Department of the Clinical Hospital Sibiu from January 11, 2011 to December 20, 2013 have been included in our study; 595 cases with emmetropia and the rest of the 1180 patients had the following refraction errors: 216 - myopia and 964 - hypermetropia. From the total cases of the hypermetropia, we selected 72 patients to measure the endothelial cells density and the corneal thickness by using specular microscopy, one day before and 7-14 days after surgery. Results and discussions: The preexisting hypermetropia might modify the intraoperative and postoperative cataract surgery evolution. Endothelial cell loss is potentially higher from surgical trauma so that the endothelium must be protected with viscoelastics. The loss of endothelial cells in hyperopic eyes occurred with an average of 267 cell/ mm² and the thickness of the cornea increased by 13 µm. Conclusion: The phacoemulsification surgery in the presence of hypermetropia requires more attention. The biometry and the specular microscopy are very important tasks for the preoperative assessment, surgery, and postoperative care. The protection of the corneal endothelium with viscoelastics leads to an insignificant modification of the endothelial cells in hyperopic patients compared to an anterior study of the patients with all ametropies.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180311
[Lr] Last revision date:180311
[St] Status:In-Process

  2 / 2047 MEDLINE  
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[PMID]: 29504956
[Au] Autor:Bhadange Y; Lautert J; Li S; Lawando E; Kim ET; Soper MC; Price FW; Price MO; Bonanno JA
[Ad] Address:Cornea Research Foundation of America, Indianapolis, IN.
[Ti] Title:Hypoxia and the Prolyl Hydroxylase Inhibitor FG-4592 Protect Corneal Endothelial Cells From Mechanical and Perioperative Surgical Stress.
[So] Source:Cornea;37(4):501-507, 2018 Apr.
[Is] ISSN:1536-4798
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PURPOSE: To determine whether hypoxia preconditioning can protect corneal endothelial cells from mechanical stress and perioperative procedures mimicking Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: Preconditioning was delivered by 2 hours of 0.5% oxygen incubation in a hypoxia chamber or by exposure to the prolyl hydroxylase inhibitor FG-4592, which prevents hypoxia-inducible factor-1 alpha degradation. Damage to whole corneas was produced by brief sonication. To mimic use with DSAEK, FG-4592-preconditioned and control donor corneas were dissected with a microkeratome, and the posterior donor button was pulled through a transplant insertion device (Busin glide). The area of endothelial damage was determined by trypan blue staining. RESULTS: In all cases, hypoxia preconditioning or incubation with FG-4592 protected corneal endothelial cells from death by mechanical stress. Hypoxia-preconditioned human and rabbit corneas showed 19% and 29% less cell loss, respectively, relative to controls, which were both significant at P < 0.05. FG-4592 preconditioning reduced endothelial cell loss associated with preparation and insertion of DSAEK grafts by 23% relative to the control (P < 0.01). CONCLUSIONS: These results support the hypothesis that preconditioning by hypoxia or exposure to FG-4592 improves corneal endothelial cell survival and may also provide protection during surgical trauma.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:In-Process
[do] DOI:10.1097/ICO.0000000000001430

  3 / 2047 MEDLINE  
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[PMID]: 29498188
[Au] Autor:Peng RM; Guo YX; Qiu Y; Hong J; Xiao GG; Qu HQ
[Ad] Address:Department of Ophthalmology, Peking University Third Hospital, Beijing, China.
[Ti] Title:Clinical outcomes after Descemet's stripping endothelial keratoplasty using donor corneas from children younger than 3 years.
[So] Source:Clin Exp Ophthalmol;, 2018 Mar 02.
[Is] ISSN:1442-9071
[Cp] Country of publication:Australia
[La] Language:eng
[Ab] Abstract:IMPORTANCE: There is limited literature on paediatric donors in endothelial keratoplasty. BACKGROUND: This study investigated the efficacy of and appropriate paediatric donor age for Descemet's stripping endothelial keratoplasty (DSEK). DESIGN: Retrospective, observational case series. PARTICIPANTS: Thirty-eight consecutive patients underwent DSEK with paediatric donor corneas. METHODS: The age of the donors ranged from 32 weeks gestation (premature neonate) to 3 years old. All donor consents were obtained from the parents. The causes of donor death included traffic accident, congenital heart disease, and neonatal respiratory distress syndrome. MAIN OUTCOME MEASURES: The outcome measures included best-corrected visual acuity (BCVA), endothelial cell loss (ECL) and complications. RESULTS: BCVA at last follow-up was >20/40 in 28 of 38 eyes (73.7%). The mean preoperative endothelial cell density (ECD) of donor corneas was 4682±520 cells/mm . The mean ECD of grafts was 3977±556 cells/mm at 18 months postoperatively. Three lenticules from premature neonate donors exhibited severe contraction postoperatively. The edge of 6 lenticules from donors <1-year-old exhibited contraction in the early postoperative period and gradually flattened spontaneously. Graft detachment occurred in one patient. CONCLUSION AND RELEVANCE: DSEK with paediatric donor corneas can achieve good clinical outcomes. The corneal lenticules from 1- to 3-year- old donors are suitable for DSEK while those from donors <1-year-old are less suitable due to the possibility of severe postoperative graft contraction.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180302
[Lr] Last revision date:180302
[St] Status:Publisher
[do] DOI:10.1111/ceo.13186

  4 / 2047 MEDLINE  
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[PMID]: 29483081
[Au] Autor:Lee WH; Tey A; Gales K; Mudhar HS; Figueiredo FC
[Ad] Address:Suite 17 Eye Clinic, Hermitage Medical Clinic, Dublin, Ireland.
[Ti] Title:Progressive idiopathic unilateral corneal endothelial failure of unknown aetiology in phakic eyes.
[So] Source:Br J Ophthalmol;, 2018 Feb 26.
[Is] ISSN:1468-2079
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:AIM: To describe a cohort of patients with irreversible unilateral bullous keratopathy (BK) of undetermined aetiology. METHOD: Retrospective, single-centre case series in a tertiary corneal referral centre. RESULTS: Eleven consecutive patients (nine females; mean age 71.7 years) presented from 1999 to 2009 with acute onset unilateral visual loss. At presentation, the best-corrected visual acuity of the affected eyes was 6/9 or worse with mean central corneal thickness (CCT) of 684 (SD 66) µm. Specular microscopy was not possible in the affected eyes. There was no other ocular pathology in the affected eye. The fellow eye remained normal throughout the study (mean endothelial cell density (ECD) of 1980 (SD 736) cells/mm and CCT of 536 (SD 34) µm). Topical steroid, antiviral treatments (both topical and systemic) or a combination of both did not yield any improvement. After a mean follow-up of 82.2 months, eight eyes had penetrating keratoplasty (PK). One required two regrafts. Histology showed typical BK features, with endothelial cell (EC) loss and thickened Descemet's membrane (DM). Transmission electron microscopy revealed DM abnormalities in a non-consistent pattern, featuring variable collagen deposition posterior to the non-banded zone. The ECs were degenerated, reduced or absent. Neither viruses nor pseudoexfoliation material was identified. CONCLUSION: While medical treatment is not beneficial, PK appears to offer good results. Non-guttate Fuchs' corneal endothelial dystrophy merits consideration but it would be unusual to see an exclusively unilateral presentation. DM thickening is reflective of a chronic EC loss but the cause of this loss remains elusive.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180227
[Lr] Last revision date:180227
[St] Status:Publisher

  5 / 2047 MEDLINE  
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[PMID]: 29422758
[Au] Autor:Liu TS; Meskin S
[Ad] Address:Department of Ophthalmology and Visual Science, Yale School of Medicine, New Haven, CT, USA.
[Ti] Title:Localized Trichiasis Causing Focal Full-Thickness Corneal Edema, Endothelial Cell Loss, and Corneal Scarring Requiring Penetrating Keratoplasty.
[So] Source:Middle East Afr J Ophthalmol;24(4):216-218, 2017 Oct-Dec.
[Is] ISSN:0975-1599
[Cp] Country of publication:India
[La] Language:eng
[Ab] Abstract:The purpose of the study was to report a case of focal trichiasis causing full-thickness corneal edema, scarring, and endothelial cell loss requiring penetrating keratoplasty (PK). A 66-year-old male was referred for trichiasis of the right upper eyelid corresponding to an area of full-thickness corneal edema. No keratic precipitates or guttata was noted. Specular microscopy showed diffuse endothelial cell loss. He was treated with topical steroids and acyclovir with epilation of lashes. Anterior chamber paracentesis was negative for varicella-zoster virus, cytomegalovirus, and herpes simplex virus. The patient developed diffuse stromal scarring with a decrease in vision and ultimately underwent PK with preceding eyelid repair. To the best of our knowledge, we present the first reported case of chronic trichiasis causing full-thickness corneal edema, scarring, and endothelial cell loss requiring PK.
[Mh] MeSH terms primary: Corneal Edema/etiology
Corneal Endothelial Cell Loss/etiology
Corneal Injuries/etiology
Keratoplasty, Penetrating
Trichiasis/complications
[Mh] MeSH terms secundary: Aged
Corneal Edema/surgery
Corneal Endothelial Cell Loss/surgery
Corneal Injuries/surgery
Humans
Male
[Pt] Publication type:CASE REPORTS; JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180223
[Lr] Last revision date:180223
[Js] Journal subset:IM
[Da] Date of entry for processing:180210
[St] Status:MEDLINE
[do] DOI:10.4103/meajo.MEAJO_304_16

  6 / 2047 MEDLINE  
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[PMID]: 29422754
[Au] Autor:Sahu PK; Das GK; Agrawal S; Kumar S; Kumar N
[Ad] Address:Department of Ophthalmology, UCMS and GTB Hospital, New Delhi, India.
[Ti] Title:Comparative Evaluation of Corneal Endothelium in Diabetic Patients Undergoing Phacoemulsification.
[So] Source:Middle East Afr J Ophthalmol;24(4):195-201, 2017 Oct-Dec.
[Is] ISSN:0975-1599
[Cp] Country of publication:India
[La] Language:eng
[Ab] Abstract:PURPOSE: To determine if phacoemulsification with intraocular lens implantation has a greater impact on the corneal endothelium of type 2 diabetic patients as compared to nondiabetics. MATERIALS AND METHODS: This study compared the endothelial status in 60 diabetics with good glycemic control and 60 nondiabetics before and after uneventful phacoemulsification. Central corneal endothelial cell density, percentage hexagonality and percentage coefficient of variation (%CV) were measured using a specular microscope. RESULTS: Data were age and sex matched. Diabetics showed significantly higher loss in endothelial cell count as compared to nondiabetics. At the end of 3 months, there was a decline of 157 endothelial cell (standard deviation [SD] 90) in the diabetic group and 121 (SD 50) in the control group. This was statistically significant ( = 0.008). Furthermore, the diabetics showed a slower recovery trend in the endothelial healing response as evidenced by lower change in the %CV. The change in %CV was 4.5 in the control group and 3.1 in diabetic group which was statistically significant ( = 0.016). Significant correlation was found between energy used and change in endothelial count as well as %CV in nondiabetics only. CONCLUSION: In spite of good glycemic control, diabetics have significantly more endothelial damage in comparison to nondiabetics with similar nuclear grading and phaco energy used. This warrants a more careful use of phaco energy in diabetics.
[Mh] MeSH terms primary: Corneal Endothelial Cell Loss/diagnosis
Diabetes Mellitus, Type 2/complications
Endothelium, Corneal/pathology
Lens Implantation, Intraocular
Phacoemulsification
Postoperative Complications
[Mh] MeSH terms secundary: Aged
Blood Glucose/metabolism
Cell Count
Corneal Endothelial Cell Loss/etiology
Female
Glycated Hemoglobin A/metabolism
Humans
Male
Middle Aged
Prospective Studies
[Pt] Publication type:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Name of substance:0 (Blood Glucose); 0 (Glycated Hemoglobin A); 0 (hemoglobin A1c protein, human)
[Em] Entry month:1802
[Cu] Class update date: 180223
[Lr] Last revision date:180223
[Js] Journal subset:IM
[Da] Date of entry for processing:180210
[St] Status:MEDLINE
[do] DOI:10.4103/meajo.MEAJO_212_16

  7 / 2047 MEDLINE  
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[PMID]: 29380761
[Au] Autor:Kapur N; Gunda S; Dixit S; Chauhan L; Acharya M; Mathur U
[Ad] Address:Dr. Shroff 's Charity Eye Hospital, New Delhi, India.
[Ti] Title:Effect of transfer of donor corneal tissue from McCarey-Kaufmann medium to Optisol-GS on corneal endothelium.
[So] Source:Indian J Ophthalmol;66(2):219-222, 2018 Feb.
[Is] ISSN:1998-3689
[Cp] Country of publication:India
[La] Language:eng
[Ab] Abstract:PURPOSE: The purpose of this study is to evaluate the effect of transfer of donor corneal tissue from McCarey-Kaufmann (MK) medium to Optisol-GS on corneal endothelium. METHODS: This was a prospective, randomized comparative study. Twenty paired human donor corneal tissues of optical quality were retrieved. One tissue of the pair was preserved in Optisol-GS preservative medium (Group A) and other tissue of the pair in MK medium (Group B) at the time of corneoscleral disc excision. Within 12 h of retrieval, each cornea was evaluated using slit-lamp biomicroscopic examination and specular microscopic analysis. Group B corneas were transferred to Optisol-GS medium within 48-53 h of retrieval. Specular analysis of the paired corneas was repeated 3 h after transferring to Optisol-GS. On day 7 of storage, specular analysis of both the tissues was repeated. RESULTS: The average age of the donor at the time of death was 29 years (16-68 years). The reduction in endothelial cell count, from baseline, in Groups A and B was 5.5% and 5.8% (P = 0.938) on the 3rd day and 8.2% and 12.6% (P = 0.025) on the 7th day, respectively, postretrieval. The coefficient of variation (CV) increased by 36% (P = 0.021) and hexagonality reduced by 19% (P = 0.007) on day 7. All tissues retained an endothelial cell density higher than the accepted critical level for penetrating keratoplasty. CONCLUSION: Significant endothelial cell loss was noted while transferring tissues from one medium to another, necessitating the need for reevaluation of transferred tissues before utilization.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180223
[Lr] Last revision date:180223
[St] Status:In-Data-Review
[do] DOI:10.4103/ijo.IJO_677_17

  8 / 2047 MEDLINE  
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[PMID]: 29450332
[Au] Autor:Módis LJ; Szalai E; Flaskó Z; Németh G
[Ad] Address:Department of Ophthalmology, Clinical Center, University of Debrecen, Debrecen, Hungary.
[Ti] Title:Corneal endothelial morphology and function after torsional and longitudinal ultrasound mode phacoemulsification.
[So] Source:Rom J Ophthalmol;60(2):109-115, 2016 Apr-Jun.
[Is] ISSN:2457-4325
[Cp] Country of publication:Romania
[La] Language:eng
[Ab] Abstract:PURPOSE: To study the endothelial cell morphology and corneal thickness changes after phacoemulsification by using the OZil torsional and longitudinal ultrasound techniques (Infiniti Vision System, Alcon Laboratories). SETTING: Department of Ophthalmology, Clinical Center, University of Debrecen, Debrecen, Hungary. METHODS: 52 patients with cataract were randomly assigned to longitudinal ultrasound and torsional mode group. All surgeries were performed through a 2.2 mm clear corneal incision, the method employed being divide and conquer. The endothelial morphometry such as cell density (ECD), mean cell area, coefficient of variation of cell area, and central corneal thickness were examined with specular microscopy (EM-1000, Tomey) preoperatively and 4, 8 weeks postoperatively. RESULTS: ECD values decreased significantly in both surgical groups (P < .001, repeated- mesures ANOVA), the postoperative endothelial cell loss was higher in the longitudinal ultrasound mode group (3.5% and 6.5%, at 4 and 8 weeks after surgery) than in the torsional group (3.3% and 5.5%, at 4 and 8 weeks after surgery), the difference not being significant between the two groups (P = .164 and P = .479, at 4 and 8 weeks after surgery, Mann-Whitney test). There was no statistically significant difference in any of the assessed parameters between the two surgical groups (P > .05). No significant correlation was found between the endothelial cell loss and the nucleus density. CONCLUSIONS: Both phacoemulsification techniques were safe and effective. The torsional handpiece performs oscillatory movements and delivers less energy into the eye than the longitudinal ultrasound technique, therefore providing more favorable energy and thermal safety profile.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180222
[Lr] Last revision date:180222
[St] Status:In-Process

  9 / 2047 MEDLINE  
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[PMID]: 29252045
[Au] Autor:Li S; Chen X; Zhao J; Xu M; Yu Z
[Ad] Address:a Aier School of Ophthalmology, Central South University , Changsha , China.
[Ti] Title:Isolated Capsulorhexis Flap Technique in Femtosecond Laser-Assisted Cataract Surgery to Protect the Corneal Endothelial Cells.
[So] Source:J Invest Surg;:1-4, 2017 Dec 18.
[Is] ISSN:1521-0553
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:PURPOSE: To present a novel technique that using capsulorhexis flap to protect corneal endothelial cells during femtosecond laser-assisted cataract surgery. METHODS: During the procedure, an isolated capsulorhexis flap was used to lift it up carefully by using OVD until the flap attached to the corneal inner layer. It kept steady during the phaco phase, and been removed by I/A tip after intraocular lens implantation. RESULTS: In our pilot study, the damage to the corneal endothelial cells from the phacoemulsification procedure were no more than the traditional phaco procedure, even femtosecond laser assisted cataract surgery. CONCLUSION: Isolated capsulorhexis flap technique initiate a new approach to protect the endothelial cell. It is a simple technique with a short learning curve, but still need a further investigation to prove the protection of corneal endothelial cells.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180221
[Lr] Last revision date:180221
[St] Status:Publisher
[do] DOI:10.1080/08941939.2017.1372537

  10 / 2047 MEDLINE  
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[PMID]: 29294389
[Au] Autor:Katikireddy KR; White TL; Miyajima T; Vasanth S; Raoof D; Chen Y; Price MO; Price FW; Jurkunas UV
[Ad] Address:Schepens Eye Research Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, 20 Staniford Street, Boston, MA 02114, USA.
[Ti] Title:NQO1 downregulation potentiates menadione-induced endothelial-mesenchymal transition during rosette formation in Fuchs endothelial corneal dystrophy.
[So] Source:Free Radic Biol Med;116:19-30, 2018 Feb 20.
[Is] ISSN:1873-4596
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Fuchs endothelial corneal dystrophy (FECD) is a genetic and oxidative stress disorder of post-mitotic human corneal endothelial cells (HCEnCs), which normally exhibit hexagonal shape and form a compact monolayer compatible with normal corneal functioning and clear vision. FECD is associated with increased DNA damage, which in turn leads to HCEnC loss, resulting in the formation rosettes and aberrant extracellular matrix (ECM) deposition in the form of pro-fibrotic guttae. Since the mechanism of ECM deposition in FECD is currently unknown, we aimed to investigate the role of endothelial-mesenchymal transition (EMT) in FECD using a previously established cellular in vitro model that recapitulates the characteristic rosette formation, by employing menadione (MN)-induced oxidative stress. We demonstrate that MN treatment alone, or a combination of MN and TGF-ß1 induces reactive oxygen species (ROS), cell death, and EMT in HCEnCs during rosette formation, resulting in upregulation of EMT- and FECD-associated markers such as Snail1, N-cadherin, ZEB1, and transforming growth factor-beta-induced (TGFßI), respectively. Additionally, FECD ex vivo specimens displayed a loss of organized junctional staining of plasma membrane-bound N-cadherin, with corresponding increase in fibronectin and Snail1 compared to ex vivo controls. Addition of N-acetylcysteine (NAC) downregulated all EMT markers and abolished rosette formation. Loss of NQO1, a metabolizing enzyme of MN, led to greater increase in intracellular ROS levels as well as a significant upregulation of Snail1, fibronectin, and N-cadherin compared to normal cells, indicating that NQO1 regulates Snail1-mediated EMT. This study provides first line evidence that MN-induced oxidative stress leads to EMT in corneal endothelial cells, and the effect of which is further potentiated when redox cycling activity of MN is enhanced by the absence of NQO1. Given that NAC inhibits Snail-mediated EMT, this may be a potential therapeutic intervention for FECD.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180220
[Lr] Last revision date:180220
[St] Status:In-Data-Review


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