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[PMID]: 29306808
[Au] Autor:Zhao S; Zhou T; Zhu L; Wang B; Li Z; Yang L; Liu L
[Ad] Address:Key Laboratory of Industrial Ecology and Environmental Engineering (MOE), School of Food and Environment, Dalian University of Technology, Panjin, Liaoning 124221, PR China. Electronic address: zhaoshuyan@dlut.edu.cn.
[Ti] Title:Uptake, translocation and biotransformation of N-ethyl perfluorooctanesulfonamide (N-EtFOSA) by hydroponically grown plants.
[So] Source:Environ Pollut;235:404-410, 2018 Apr.
[Is] ISSN:1873-6424
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:N-ethyl perfluorooctane sulfonamide (N-EtFOSA) is an important perfluorooctanesulfonate (PFOS) precursor (PreFOS) which is used in sulfluramid. The present work studied the uptake, translocation and metabolism of N-EtFOSA in wheat (Triticum aestivum L.), soybean (Glycine max L. Merrill) and pumpkin (Cucurbita maxima L.) by hydroponic exposure. Except for parent N-EtFOSA, its metabolites of perfluorooctane sulfonamide acetate (FOSAA), perfluorooctane sulfonamide (PFOSA), PFOS, perfluorohexane sulfonate (PFHxS) and perfluorobutane sulfonate (PFBS) were detected in the roots and shoots of all the three plant species examined. This suggested that plant roots could take up N-EtFOSA from solutions efficiently, and translocate to shoots. A positive correlation was found between root concentration factors (RCFs) of N-EtFOSA and root lipid content. Much higher proportion of N-EtFOSA transformation products in plant tissues than in the solutions suggested that N-EtFOSA could be in vivo metabolized in plant roots and shoots to FOSAA, PFOSA and PFOS, and other additional shorter-chain perfluoroalkane sulfonates (PFSAs), including PFHxS and PFBS. The results suggested that plants had biotransformation pathways to N-EtFOSA that were different than those from microorganisms and animals. This study provides important information about the uptake and metabolism of PreFOSs in plants.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180302
[Lr] Last revision date:180302
[St] Status:In-Process

  2 / 1728 MEDLINE  
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[PMID]: 29473382
[Au] Autor:Vecchioli-Scaldazza C; Morosetti C; Maruccia S; Casellato S; Rociola W; Illiano E; Garofalo F
[Ad] Address:Uroginecology Unit, ASUR n° 2 Jesi (AN). cascave@alice.it.
[Ti] Title:A randomized, multicenter, controlled study, comparing efficacy and safety of a new complementary and alternative medicine (CAM) versus Solifenacin Succinate in women with overactive bladder syndrome.
[So] Source:Arch Ital Urol Androl;89(4):296-300, 2017 Dec 31.
[Is] ISSN:1124-3562
[Cp] Country of publication:Italy
[La] Language:eng
[Ab] Abstract:INTRODUCTION: To assess efficacy and tolerability of a new complementary and alternative medicine (CAM) consisting of vitamins (C and D), herbal products (cucurbita maxima, capsicum annum, polygonum capsicatum) and amino acid L-Glutammina, in the treatment of female Overactive Bladder syndrome (OAB). MATERIALS AND METHODS: 90 consecutive women with OAB symptoms were enrolled in this prospective, randomized, controlled study. Women were divided randomly into two groups of 45 patients each. In group A, women received Solifenacin Succinate (SS), 5 mg. once a day for 12 weeks. In group B, women received CAM, 930 mg, twice daily for 12 weeks. Women were assessed with 3-day micturition diary, Patient Perception of Intensity of Urgency Scale (PPIUS), Overactive Bladder questionnaire Short Form (OAB-q SF) and Patient Global Impression of Improvement questionnaire (PGI-I). RESULTS: 8 patients in group A and 1 patient in group B dropped out from therapy because of side effects. A reduction in the number of daily micturitions, nocturia and episodes of urge incontinence was present with both SS and CAM with statistically highly significant differences, but CAM was significantly more effective than SS. PPIUS and OAB-q SF showed improvements with both SS and CAM with a more significant efficacy of CAM. PGI-I, demonstrated improvements in the two groups of patients with a greater satisfaction expressed by patients treated with CAM. CONCLUSIONS: the small number of patients does not permit definitive conclusions; however, the results of the research showed the greater effectiveness and tolerability of CAM.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180223
[Lr] Last revision date:180223
[St] Status:In-Data-Review
[do] DOI:10.4081/aiua.2017.4.296

  3 / 1728 MEDLINE  
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[PMID]: 29196817
[Au] Autor:Perotto MC; Pozzi EA; Celli MG; Luciani CE; Mitidieri MS; Conci VC
[Ad] Address:Instituto de Patología Vegetal (IPAVE-CIAP-INTA), Camino 60 cuadras km 5,5, Córdoba, X5020ICA, Argentina. perotto.cecilia@inta.gob.ar.
[Ti] Title:Identification and characterization of a new potyvirus infecting cucurbits.
[So] Source:Arch Virol;163(3):719-724, 2018 Mar.
[Is] ISSN:1432-8798
[Cp] Country of publication:Austria
[La] Language:eng
[Ab] Abstract:A new potyvirus, tentatively named cucurbit vein banding virus (CVBV), was identified in crops of cucurbits in San Pedro (Buenos Aires, Argentina). The complete genome sequences of two isolates of CVBV were obtained by next-generation sequencing (Illumina). The genomic RNA consisted of 9968 and 9813 nucleotides, respectively, and displayed typical potyvirus organization. The percentage identity for these two genome sequences, using BLASTn, was 77% to sweet potato virus c and 73% to tomato necrotic stunt virus. BLASTx analysis of the complete polyprotein showed that the most closely related virus is plum pox virus, with 48% amino acid sequence identity for both isolates. Sequence comparisons and phylogenetic analyses indicate that CVBV belongs to a previously undescribed species in genus Potyvirus.
[Mh] MeSH terms primary: Cucurbita/virology
Genome, Viral
Phylogeny
Potyvirus/genetics
RNA, Viral/genetics
[Mh] MeSH terms secundary: Argentina
Base Sequence
High-Throughput Nucleotide Sequencing
Open Reading Frames
Plant Diseases/virology
Potyvirus/classification
Potyvirus/isolation & purification
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (RNA, Viral)
[Em] Entry month:1802
[Cu] Class update date: 180220
[Lr] Last revision date:180220
[Js] Journal subset:IM
[Da] Date of entry for processing:171203
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3660-2

  4 / 1728 MEDLINE  
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[PMID]: 29458586
[Au] Autor:Iyer RI; Panda T
[Ad] Address:Biochemical Engineering Laboratory, Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600036, Tamil Nadu, India.
[Ti] Title:Biosynthesis of Gold and Silver Nanoparticles Using Extracts of Callus Cultures of Pumpkin ( ).
[So] Source:J Nanosci Nanotechnol;18(8):5341-5353, 2018 Aug 01.
[Is] ISSN:1533-4880
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The potential of callus cultures and field-grown organs of pumpkin (Cucurbita maxima) for the biosynthesis of nanoparticles of the noble metals gold and silver has been investigated. Biosynthesis of AuNPs (gold nanoparticles) and AgNPs (silver nanoparticles) was obtained with flowers of C. maxima but not with pulp and seeds. With callus cultures established in MS-based medium the biogenesis of both AuNPs and AgNPs could be obtained. At 65 °C the biogenesis of AuNPs and AgNPs by callus extracts was enhanced. The AuNPs and AgNPs have been characterized by UV-visible spectroscopy, TEM, DLS and XRD. Well-dispersed nanoparticles, which exhibited a remarkable diversity in size and shape, could be visualized by TEM. Gold nanoparticles were found to be of various shapes, viz., rods, triangles, star-shaped particles, spheres, hexagons, bipyramids, discoid particles, nanotrapezoids, prisms, cuboids. Silver nanoparticles were also of diverse shapes, viz., discoid, spherical, elliptical, triangle-like, belt-like, rod-shaped forms and cuboids. EDX analysis indicated that the AuNPs and AgNPs had a high degree of purity. The surface charges of the generated AuNPs and AgNPs were highly negative as indicated by zeta potential measurements. The AuNPs and AgNPs exhibited remarkable stability in solution for more than four months. FTIR studies indicated that biomolecules in the callus extracts were associated with the biosynthesis and stabilisation of the nanoparticles. The synthesized AgNPs could catalyse degradation of methylene blue and exhibited anti-bacterial activity against E. coli DH5α. There is no earlier report of the biosynthesis of nanoparticles by this plant species. Callus cultures of Cucurbita maxima are effective alternative resources of biomass for synthesis of nanoparticles.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180220
[Lr] Last revision date:180220
[St] Status:In-Process
[do] DOI:10.1166/jnn.2018.15378

  5 / 1728 MEDLINE  
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[PMID]: 29262705
[Au] Autor:Bohm V; Fekete D; Balázs G; Gáspár L; Kappel N
[Ti] Title:Salinity tolerance of grafted watermelon seedlings.
[So] Source:Acta Biol Hung;68(4):412-427, 2017 Dec.
[Is] ISSN:0236-5383
[Cp] Country of publication:Hungary
[La] Language:eng
[Ab] Abstract:In order to evaluate the salinity tolerance of grafted watermelon, two sets of experiments were conducted in a growing chamber where 'Esmeralda' varieties were grafted onto interspecific squash (Cucurbita maxima Duch. × Cucurbita moschata Duch.) and Lagenaria siceraria rootstocks. Both non-grafted and self-garfted plants were used for control. For salt stress, 2.85 and 4.28 mM/l substrate doses of NaCl were added with each irrigation in 2 day intervals for a duration of 23 days. Interspecific-grafted plants showed the highest salinity tolerance as plant biomass and leaf area were not decreased but improved by salinity in most cases. Furthermore, transpiration and photosynthesis activity did not decrease as much as it did in the case of other grafting combinations. Interspecific and Lagenaria rootstocks showed sodium retention, as elevation of Na content in the leaves of these grafting combinations was negligible compared to self-grafted and non-grafted ones. Presumably abiotic stress tolerance can be enhanced by grafting per se considering measured parameters of self-grafted plants did not decrease as much as seen in non-grafted ones.
[Mh] MeSH terms primary: Citrullus/metabolism
Photosynthesis/physiology
Salt-Tolerance/physiology
Seedlings/metabolism
[Mh] MeSH terms secundary: Biomass
Plant Leaves/metabolism
Plant Roots/metabolism
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180216
[Lr] Last revision date:180216
[Js] Journal subset:IM
[Da] Date of entry for processing:171222
[St] Status:MEDLINE
[do] DOI:10.1556/018.68.2017.4.7

  6 / 1728 MEDLINE  
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[PMID]: 29415652
[Au] Autor:Carvajal F; Rosales R; Palma F; Manzano S; Cañizares J; Jamilena M; Garrido D
[Ad] Address:Department of Plant Physiology, Facultad de Ciencias, University of Granada, Fuentenueva s/n, 18071, Granada, Spain.
[Ti] Title:Transcriptomic changes in Cucurbita pepo fruit after cold storage: differential response between two cultivars contrasting in chilling sensitivity.
[So] Source:BMC Genomics;19(1):125, 2018 02 07.
[Is] ISSN:1471-2164
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: Zucchini fruit is susceptible to chilling injury (CI), but the response to low storage temperature is cultivar dependent. Previous reports about the response of zucchini fruit to chilling storage have been focused on the physiology and biochemistry of this process, with little information about the molecular mechanisms underlying it. In this work, we present a comprehensive analysis of transcriptomic changes that take place after cold storage in zucchini fruit of two commercial cultivars with contrasting response to chilling stress. RESULTS: RNA-Seq analysis was conducted in exocarp of fruit at harvest and after 14 days of storage at 4 and 20 °C. Differential expressed genes (DEGs) were obtained comparing fruit stored at 4 °C with their control at 20 °C, and then specific and common up and down-regulated DEGs of each cultivar were identified. Functional analysis of these DEGs identified similarities between the response of zucchini fruit to low temperature and other stresses, with an important number of GO terms related to biotic and abiotic stresses overrepresented in both cultivars. This study also revealed several molecular mechanisms that could be related to chilling tolerance, since they were up-regulated in cv. Natura (CI tolerant) or down-regulated in cv. Sinatra (CI sensitive). These mechanisms were mainly those related to carbohydrate and energy metabolism, transcription, signal transduction, and protein transport and degradation. Among DEGs belonging to these pathways, we selected candidate genes that could regulate or promote chilling tolerance in zucchini fruit including the transcription factors MYB76-like, ZAT10-like, DELLA protein GAIP, and AP2/ERF domain-containing protein. CONCLUSIONS: This study provides a broader understanding of the important mechanisms and processes related to coping with low temperature stress in zucchini fruit and allowed the identification of some candidate genes that may be involved in the acquisition of chilling tolerance in this crop. These genes will be the basis of future studies aimed to identify markers involved in cold tolerance and aid in zucchini breeding programs.
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1802
[Cu] Class update date: 180216
[Lr] Last revision date:180216
[St] Status:In-Process
[do] DOI:10.1186/s12864-018-4500-9

  7 / 1728 MEDLINE  
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[PMID]: 29320569
[Au] Autor:Guo WL; Chen BH; Chen XJ; Guo YY; Yang HL; Li XZ; Wang GY
[Ad] Address:School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xin Xiang, China.
[Ti] Title:Transcriptome profiling of pumpkin (Cucurbita moschata Duch.) leaves infected with powdery mildew.
[So] Source:PLoS One;13(1):e0190175, 2018.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Cucurbit powdery mildew (PM) is one of the most severe fungal diseases, but the molecular mechanisms underlying PM resistance remain largely unknown, especially in pumpkin (Cucurbita moschata Duch.). The goal of this study was to identify gene expression differences in PM-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line "112-2" using RNA sequencing (RNA-Seq). The inbred line "112-2" has been purified over 8 consecutive generations of self-pollination and shows high resistance to PM. More than 7600 transcripts were examined in pumpkin leaves, and 3129 and 3080 differentially expressed genes (DEGs) were identified in inbred line "112-2" at 24 and 48 hours post inoculation (hpi), respectively. Based on the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway database and GO (Gene Ontology) database, a complex regulatory network for PM resistance that may involve hormone signal transduction pathways, transcription factors and defense responses was revealed at the transcription level. In addition, the expression profiles of 16 selected genes were analyzed using quantitative RT-PCR. Among these genes, the transcript levels of 6 DEGs, including bHLH87 (Basic Helix-loop-helix transcription factor), ERF014 (Ethylene response factor), WRKY21 (WRKY domain), HSF (heat stress transcription factor A), MLO3 (Mildew Locus O), and SGT1 (Suppressor of G-Two Allele of Skp1), in PM-resistant "112-2" were found to be significantly up- or down-regulated both before 9 hpi and at 24 hpi or 48 hpi; this behavior differed from that observed in the PM-susceptible material (cultivar "Jiujiangjiaoding"). The transcriptome data provide novel insights into the response of Cucurbita moschata to PM stress and are expected to be highly useful for dissecting PM defense mechanisms in this major vegetable and for improving pumpkin breeding with enhanced resistance to PM.
[Mh] MeSH terms primary: Ascomycota/physiology
Cucurbita/genetics
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
Plant Diseases/genetics
Plant Leaves/metabolism
[Mh] MeSH terms secundary: Disease Resistance
Gene Library
Gene Ontology
Metabolic Networks and Pathways/genetics
Photosynthesis/genetics
Plant Growth Regulators/physiology
Plant Leaves/microbiology
RNA, Plant/biosynthesis
RNA, Plant/genetics
Sequence Analysis, RNA
Signal Transduction/genetics
Transcription Factors/genetics
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Plant Growth Regulators); 0 (RNA, Plant); 0 (Transcription Factors)
[Em] Entry month:1802
[Cu] Class update date: 180214
[Lr] Last revision date:180214
[Js] Journal subset:IM
[Da] Date of entry for processing:180111
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190175

  8 / 1728 MEDLINE  
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[PMID]: 29253565
[Au] Autor:Lee W; Ko KR; Kim HK; Lim S; Kim S
[Ad] Address:Department of Biological Sciences, Seoul National University, Seoul 151-742, South Korea; ViroMed Co., Ltd., Seoul 151-747, South Korea.
[Ti] Title:Dehydrodiconiferyl alcohol promotes BMP-2-induced osteoblastogenesis through its agonistic effects on estrogen receptor.
[So] Source:Biochem Biophys Res Commun;495(3):2242-2248, 2018 01 15.
[Is] ISSN:1090-2104
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Estrogen deficiency results in an imbalance between the levels of bone-resorping osteoclasts and bone-forming osteoblasts, eventually leading to overall bone loss. Dehydrodiconiferyl alcohol (DHCA), a lignan compound originally isolated from Cucurbita moschata, has been shown to bind to estrogen receptor, and indeed exhibits various activities of estrogen, such as anti-inflammatory and anti-oxidative stress effects. In this study, we tested whether synthetic DHCA could affect the BMP-2-induced osteoblastogenesis in vitro. In MC3T3-E1 cells, DHCA promoted BMP-2-induced differentiation of osteoblasts. Consistently, the expression of three osteoblastogenic genes known to be induced by BMP-2, ALP, osteocalcin and OPG, was up-regulated by DHCA treatment. DHCA was also shown to activate the production of RUNX2 by activating Smad1/5/9 and AMPK. Data from transient transfection assays suggested that DHCA might activate the estrogen receptor signaling pathway. Effects of DHCA on BMP-2-induced osteoblastogenesis were reduced when cells were treated with a specific siRNA to ERα or ERß. Taken together, our results suggest that DHCA may be developed as an efficient therapeutic for osteoporosis by regulating osteoblastogenesis through its estrogenic effects.
[Mh] MeSH terms primary: Bone Morphogenetic Protein 2/metabolism
Estrogen Receptor Modulators/administration & dosage
Estrogens/metabolism
Osteoblasts/physiology
Osteogenesis/physiology
Phenols/administration & dosage
Receptors, Estrogen/metabolism
[Mh] MeSH terms secundary: 3T3 Cells
Animals
Cell Differentiation/drug effects
Cell Differentiation/physiology
Dose-Response Relationship, Drug
Mice
Osteoblasts/cytology
Osteoblasts/drug effects
Osteogenesis/drug effects
Receptors, Estrogen/drug effects
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Bmp2 protein, mouse); 0 (Bone Morphogenetic Protein 2); 0 (Estrogen Receptor Modulators); 0 (Estrogens); 0 (Phenols); 0 (Receptors, Estrogen); 4263-87-0 (dehydrodiconiferyl alcohol)
[Em] Entry month:1802
[Cu] Class update date: 180214
[Lr] Last revision date:180214
[Js] Journal subset:IM
[Da] Date of entry for processing:171219
[St] Status:MEDLINE

  9 / 1728 MEDLINE  
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[PMID]: 29438476
[Au] Autor:Buchanan AL; Hooks CRR
[Ad] Address:Department of Entomology, University of Maryland, College Park, MD.
[Ti] Title:Influence of Winter Cover Crop Mulch on Arthropods in a Reduced Tillage Cucurbit System.
[So] Source:Environ Entomol;, 2018 Feb 09.
[Is] ISSN:1938-2936
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Winter cover crop mulches can diversify agricultural habitats and provide a range of benefits for crop production and pest management. Here we report the influence of strip tilled winter cover crop mulches on arthropod abundance in organic vegetable plots. Crookneck squash (Cucurbita pepo L.; Cucurbitales: Cucurbitaceae) was direct seeded into mowed and strip tilled barley (Hordeum vulgare L.; Poales: Poaceae), crimson clover (Trifolium incarnatum L.; Fabales: Fabaceae), a barley + crimson clover mixture, or a no-cover crop control. Arthropods on squash plants were assessed weekly using visual counts. Seed predation was assessed using weed seed arenas. In 2013, mixed species cover crops produced the most ground cover, fewest weeds, and largest squash plants, but herbivore and predator abundance were not correlated with any of those factors. In 2014, mixed species cover crops again produced the most ground cover and fewest weeds, but the largest squash plants were found in no-cover crop control plots, which also had the highest herbivore abundance per plant. Predator and herbivore abundance were positively correlated with squash plant size in 2014. There were no differences in seed predation across treatments. Differences in ground cover biomass and weed presence between the 2 yr may have contributed to differences in squash plant quality and subsequent herbivore abundance between seasons. Results suggest that arthropods on plants responded largely indirectly to cover crops through host plant quality. Results are interpreted in light of overall costs and benefits of cover cropping.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180213
[Lr] Last revision date:180213
[St] Status:Publisher
[do] DOI:10.1093/ee/nvy004

  10 / 1728 MEDLINE  
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[PMID]: 29401224
[Au] Autor:O'Hearn JS; Walsh DB
[Ad] Address:Department of Entomology, Washington State University's Irrigated Agriculture Research and Extension Center, Prosser, WA.
[Ti] Title:Pumpkin as an Alternate Host Plant for Laboratory Colonies of Grape Mealybug.
[So] Source:J Econ Entomol;, 2018 Feb 01.
[Is] ISSN:1938-291X
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:The grape mealybug, Pseudococcus maritimus (Ehrhorn; Hemiptera: Pseudococcidae), is the primary vector of Grapevine Leafroll associated Viruses (GLRaVs) in Washington State vineyards. Rearing laboratory colonies of grape mealybug has proven difficult. Several host plants were tested to determine their suitability for use as an alternate host plants for laboratory colonies of grape mealybug. Of the plants tested, colonies of grape mealybug were successful on pumpkin (Cucurbita pepo cv 'Connecticut field') leaves and vines. Mealybugs were able to develop from the crawler stage, through developmental instars, and adults were able to reproduce viable offspring. To date this is the only successful study to raise grape mealybugs on a cucurbit. Pumpkin appears to be a viable alternate host plant for laboratory colonies of the grape mealybug.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180205
[Lr] Last revision date:180205
[St] Status:Publisher
[do] DOI:10.1093/jee/toy003


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