Database : MEDLINE
Search on : Cyclosporiasis [Words]
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[PMID]: 29274312
[Au] Autor:Guo Y; Li N; Ortega YR; Zhang L; Roellig DM; Feng Y; Xiao L
[Ad] Address:College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong, 510642, China; Division of Foodborne, Waterborne and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, 30329, USA.
[Ti] Title:Population genetic characterization of Cyclospora cayetanensis from discrete geographical regions.
[So] Source:Exp Parasitol;184:121-127, 2018 Jan.
[Is] ISSN:1090-2449
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Cyclospora cayetanensis is an emerging pathogen that is endemic in developing countries and responsible for many large foodborne cyclosporiasis outbreaks in North America since 1990s. Because of the lack of typing targets, the genetic diversity and population genetics of C. cayetanensis have not been investigated. In this study, we undertook a population genetic analysis of multilocus sequence typing data we recently collected from 64 C. cayetanensis specimens. Despite the extensive genetic heterogeneity in the overall C. cayetanensis population, there were significant intra- and inter-genic linkage disequilibria (LD). A disappearance of LD was observed when only multilocus genotypes were included in the population genetic analysis, indicative of an epidemic nature of C. cayetanensis. Geographical segregation-associated sub-structuring was observed between specimens from China and those from Peru and the United States. The two subpopulations had reduced LD, indicating the likely occurrence of genetic exchange among isolates in endemic areas. Further analyses of specimens from other geographical regions are necessary to fully understand the population genetics of C. cayetanensis.
[Mh] MeSH terms primary: Cyclospora/genetics
Genetics, Population
[Mh] MeSH terms secundary: Alleles
China
Cyclospora/classification
Cyclosporiasis/parasitology
Genetic Variation
Humans
Multilocus Sequence Typing
Nepal
Peru
Polymorphism, Genetic
Spain
United States
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180205
[Lr] Last revision date:180205
[Js] Journal subset:IM
[Da] Date of entry for processing:171224
[St] Status:MEDLINE

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[PMID]: 29234679
[Au] Autor:Bilung LM; Tahar AS; Yunos NE; Apun K; Lim YA; Nillian E; Hashim HF
[Ad] Address:Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia.
[Ti] Title:Detection of and Oocysts from Environmental Water for Drinking and Recreational Activities in Sarawak, Malaysia.
[So] Source:Biomed Res Int;2017:4636420, 2017.
[Is] ISSN:2314-6141
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Cryptosporidiosis and cyclosporiasis are caused by waterborne coccidian protozoan parasites of the genera and respectively. This study was conducted to detect and oocysts from environmental water abstracted by drinking water treatment plants and recreational activities in Sarawak, Malaysia. Water samples (12 each) were collected from Sungai Sarawak Kanan in Bau and Sungai Sarawak Kiri in Batu Kitang, respectively. In addition, 6 water samples each were collected from Ranchan Recreational Park and UNIMAS Lake at Universiti Malaysia Sarawak, Kota Samarahan, respectively. Water physicochemical parameters were also recorded. All samples were concentrated by the iron sulfate flocculation method followed by the sucrose floatation technique. and were detected by modified Ziehl-Neelsen technique. Correlation of the parasites distribution with water physicochemical parameters was analysed using bivariate Pearson correlation. Based on the 24 total samples of environmental water abstracted by drinking water treatment plants, all the samples (24/24; 100%) were positive with , and only 2 samples (2/24; 8.33%) were positive with . Based on the 12 total samples of water for recreational activities, 4 samples (4/12; 33%) were positive with , while 2 samples (2/12; 17%) were positive with . oocysts were negatively correlated with dissolved oxygen (DO).
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 171220
[Lr] Last revision date:171220
[St] Status:In-Process
[do] DOI:10.1155/2017/4636420

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[PMID]: 29113617
[Au] Autor:Qvarnstrom Y; Benedict T; Marcet PL; Wiegand RE; Herwaldt BL; da Silva AJ
[Ad] Address:Division of Parasitic Diseases and Malaria,Center for Global Health, Centers for Disease Control and Prevention,1600 Clifton Rd, Atlanta, GA 30329,USA.
[Ti] Title:Molecular detection of Cyclospora cayetanensis in human stool specimens using UNEX-based DNA extraction and real-time PCR.
[So] Source:Parasitology;:1-6, 2017 Nov 08.
[Is] ISSN:1469-8161
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Cyclospora cayetanensis is a coccidian parasite associated with diarrheal illness. In the USA, foodborne outbreaks of cyclosporiasis have been documented almost every year since the mid-1990s. The typical approach used to identify this parasite in human stools is an examination of acid-fast-stained smears under bright-field microscopy. UV fluorescence microscopy of wet mounts is more sensitive and specific than acid-fast staining but requires a fluorescence microscope with a special filter not commonly available in diagnostic laboratories. In this study, we evaluated a new DNA extraction method based on the Universal Nucleic Acid Extraction (UNEX) buffer and compared the performances of four published real-time polymerase chain reaction (PCR) assays for the specific detection of C. cayetanensis in stool. The UNEX-based method had an improved capability to recover DNA from oocysts compared with the FastDNA stool extraction method. The best-performing real-time PCR assay was a C. cayetanensis-specific TaqMan PCR that targets the 18S ribosomal RNA gene. This new testing algorithm should be useful for detection of C. cayetanensis in human stool samples.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1711
[Cu] Class update date: 171108
[Lr] Last revision date:171108
[St] Status:Publisher
[do] DOI:10.1017/S0031182017001925

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[PMID]: 28876133
[Au] Autor:Whitfield Y; Johnson K; Hanson H; Huneault D
[Ad] Address:1 Public Health Ontario, 480 University Avenue, Suite 300, Toronto, Ontario, Canada M5G 1V2 (ORCID: http://orcid.org/000-0002-4361-1640 [Y.W.]).
[Ti] Title:2015 Outbreak of Cyclosporiasis Linked to the Consumption of Imported Sugar Snap Peas in Ontario, Canada.
[So] Source:J Food Prot;80(10):1666-1669, 2017 Oct.
[Is] ISSN:1944-9097
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:An outbreak of cyclosporiasis in Ontario, Canada, was investigated in the fall of 2015. Thirty-five confirmed and 10 probable cases were linked to the investigation. Epidemiological and food safety evidence implicated fresh sugar snap peas imported from Guatemala as the source of the outbreak. We describe here the first documented cyclosporiasis outbreak in Canada involving the consumption of sugar snap peas.
[Mh] MeSH terms primary: Cyclosporiasis/epidemiology
Peas/parasitology
[Mh] MeSH terms secundary: Cyclospora
Disease Outbreaks
Guatemala
Humans
Ontario/epidemiology
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1711
[Cu] Class update date: 171102
[Lr] Last revision date:171102
[Js] Journal subset:IM
[Da] Date of entry for processing:170907
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-17-084

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[PMID]: 28816651
[Au] Autor:Marques DFP; Alexander CL; Chalmers RM; Elson R; Freedman J; Hawkins G; Lo J; Robinson G; Russell K; Smith-Palmer A; Kirkbride H
[Ad] Address:Health Protection Scotland (HPS), Glasgow, United Kingdom.
[Ti] Title:Cyclosporiasis in travellers returning to the United Kingdom from Mexico in summer 2017: lessons from the recent past to inform the future.
[So] Source:Euro Surveill;22(32), 2017 Aug 03.
[Is] ISSN:1560-7917
[Cp] Country of publication:Sweden
[La] Language:eng
[Ab] Abstract:During the summers of 2015 and 2016, the United Kingdom experienced large outbreaks of cyclosporiasis in travellers returning from Mexico. As the source of the outbreaks was not identified, there is the potential for a similar outbreak to occur in 2017; indeed 78 cases had already been reported as at 27 July 2017. Early communication and international collaboration is essential to provide a better understanding of the source and extent of this recurring situation.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1708
[Cu] Class update date: 170817
[Lr] Last revision date:170817
[St] Status:In-Process

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[PMID]: 28716461
[Au] Autor:Thapa SS; Basnyat B
[Ad] Address:Department of Internal Medicine, Saint Vincent Hospital, University Of Massachusetts Medical School,Worcester. Electronic address: simant.thapa@stvincenthospital.com.
[Ti] Title:Chronic Diarrhea in a Traveler: Cyclosporiasis.
[So] Source:Am J Med;, 2017 Jul 14.
[Is] ISSN:1555-7162
[Cp] Country of publication:United States
[La] Language:eng
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1707
[Cu] Class update date: 170820
[Lr] Last revision date:170820
[St] Status:Publisher

  7 / 272 MEDLINE  
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[PMID]: 28697813
[Au] Autor:Li J; Chang Y; Shi KE; Wang R; Fu K; Li S; Xu J; Jia L; Guo Z; Zhang L
[Ad] Address:College of Animal Science and Veterinary Medicine, Henan Agricultural University,Zhengzhou 450002,China.
[Ti] Title:Multilocus sequence typing and clonal population genetic structure of Cyclospora cayetanensis in humans.
[So] Source:Parasitology;144(14):1890-1897, 2017 Dec.
[Is] ISSN:1469-8161
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:To investigate the prevalence of Cyclospora cayetanensis in a longitudinal study and to conduct a population genetic analysis, fecal specimens from 6579 patients were collected during the cyclosporiasis - prevalent seasons in two urban areas of central China in 2011-2015. The overall incidence of C. cayetanensis infection was 1·2% (76/6579): 1·6% (50/3173) in Zhengzhou and 0·8% (26/3406) in Kaifeng (P 0·05). All the isolates clustered in the C. cayetanensis clade based on the small subunit ribosomal RNA gene sequence phylogenetic analysis. There were 45 specimens positive for all the five C. cayetanensis microsatellite loci, and formed 29 multilocus genotypes (MLGs). The phylogenetic relationships of 54 distinct MLGs (including 25 known reference MLGs), based on the concatenated multilocus sequences, formed three main clusters. A population structure analysis showed that the 79 isolates (including 34 known reference isolates) of C. cayetanensis produced three distinct subpopulations based on allelic profile data. In conclusion, we determined the frequency of C. cayetanensis infection in humans in Henan Province. The clonal population structure of the human C. cayetanensis isolates showed linkage disequilibrium and three distinct subpopulations.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1707
[Cu] Class update date: 171101
[Lr] Last revision date:171101
[St] Status:In-Process
[do] DOI:10.1017/S0031182017001299

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[PMID]: 28682780
[Au] Autor:Kludkowska M; Pielok L; Frackowiak K; Paul M
[Ad] Address:.
[Ti] Title:Intestinal coccidian parasites as an underestimated cause of travellers' diarrhoea in Polish immunocompetent patients.
[So] Source:Acta Parasitol;62(3):630-638, 2017 Sep 26.
[Is] ISSN:1896-1851
[Cp] Country of publication:Poland
[La] Language:eng
[Ab] Abstract:Intestinal coccidian parasites are intracellular protozoa most frequently transmitted during food-borne and water-borne infections. This group of parasites is responsible for acute diarrhoeal illnesses especially among immunocompromised patients. However, they are more frequently detected in immunocompetent individuals including travellers, and they should also be considered as important etiologic factors of travellers' diarrhoea. We examined 221 immunocompetent patients hospitalized due to acute or chronic diarrhoea and other gastrointestinal symptoms after returning from international journeys to hot climates. A basic microscopical examination and acid - fast staining of stool samples was performed. Each patient was also a part of the epidemiological investigation to define potential risk factors of tropical gastrointestinal infections. Intestinal coccidiosis was confirmed in 12 out of 221 successively hospitalized patients (5.4%). The most common coccidian parasite was Cryptosporidium spp., detected in nine Polish travellers (4.1%). Cyclospora spp. was diagnosed in three cases (1.4%), including two mixed infections with Cryptosporidium spp., and Cystoisospora spp. in two other cases (0.9%). The study has revealed that intestinal coccidian parasites are a significant threat to immunocompetent travellers and should be always considered in the differential diagnosis of gastrointestinal disorders. Therefore, it is necessary to perform specialized diagnostic methods for the detection of Cryptosporidium spp., Cystoisospora spp., and Cyclospora spp. oocysts in reference parasitology laboratories. Clinical observations demonstrated simultaneously an insufficient level of knowledge in Polish tourists concerning the main risk factors of intestinal parasitic diseases during international travels, particularly to developing countries with lower economic and sanitary conditions.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1707
[Cu] Class update date: 170706
[Lr] Last revision date:170706
[St] Status:In-Process

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[PMID]: 28682770
[Au] Autor:Uysal HK; Adas GT; Atalik K; Altiparmak S; Akgul O; Saribas S; Gurcan M; Yuksel P; Yildirmak T; Kocazeybek B; Ziver T; Oner YA
[Ad] Address:.
[Ti] Title:The Prevalence of Cyclospora cayetanensis and Cryptosporidium spp. in Turkish patients infected with HIV-1.
[So] Source:Acta Parasitol;62(3):557-564, 2017 Sep 26.
[Is] ISSN:1896-1851
[Cp] Country of publication:Poland
[La] Language:eng
[Ab] Abstract:Opportunistic infections such as cryptosporidiosis and cyclosporiasis are commonly encountered in patients with acquired immunodeficiency syndrome (AIDS). We investigated the existence of opportunistic protozoans that significantly affect the quality of life in HIV-1 infected patients using conventional and molecular methods. The study group comprised 115 HIV-1 positive patients. In the identification of Cyclospora cayetanensis and Cryptosporidium, the formol-ether precipitation method was used and smears were evaluated in optical microscope by staining modified Ziehl-Neelsen (ZN). The primers and probes used for PCR were Heat shock protein 70 for C. cayetanensis and the oocysts wall protein for Cryptosporidium spp.. Cyclospora and Cryptosporidium spp. oocysts were detected in one and two patients, respectively, by staining, whereas we detected C. cayetanensis in three patients out of 115 (2.6%) by PCR, and Cryptosporidium spp. in a further three patients (2.6%). C. cayetensis was detected in patients with CD4 counts of 64 cells/µm, 182 cells/µm and 287 cells/µm, respectively. Cryptosporidium spp. was detected in patients with CD4 counts of 176 cells/µm, 241 cells/µm and 669 cells/µm. As conclusion, PCR method is faster and more sensitive than microscopic methods and to screen intestinal pathogens routinely in patients infected with HIV should not be neglected in developing countries like Turkey.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1707
[Cu] Class update date: 170706
[Lr] Last revision date:170706
[St] Status:In-Process

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[PMID]: 28590822
[Au] Autor:Murphy HR; Lee S; da Silva AJ
[Ad] Address:U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Division of Food and Environmental Microbiology, Laurel, Maryland 20708, USA.
[Ti] Title:Evaluation of an Improved U.S. Food and Drug Administration Method for the Detection of Cyclospora cayetanensis in Produce Using Real-Time PCR.
[So] Source:J Food Prot;80(7):1133-1144, 2017 Jul.
[Is] ISSN:1944-9097
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Cyclospora cayetanensis is a protozoan parasite that causes human diarrheal disease associated with the consumption of fresh produce or water contaminated with C. cayetanensis oocysts. In the United States, foodborne outbreaks of cyclosporiasis have been linked to various types of imported fresh produce, including cilantro and raspberries. An improved method was developed for identification of C. cayetanensis in produce at the U.S. Food and Drug Administration. The method relies on a 0.1% Alconox produce wash solution for efficient recovery of oocysts, a commercial kit for DNA template preparation, and an optimized TaqMan real-time PCR assay with an internal amplification control for molecular detection of the parasite. A single laboratory validation study was performed to assess the method's performance and compare the optimized TaqMan real-time PCR assay and a reference nested PCR assay by examining 128 samples. The samples consisted of 25 g of cilantro or 50 g of raspberries seeded with 0, 5, 10, or 200 C. cayetanensis oocysts. Detection rates for cilantro seeded with 5 and 10 oocysts were 50.0 and 87.5%, respectively, with the real-time PCR assay and 43.7 and 94.8%, respectively, with the nested PCR assay. Detection rates for raspberries seeded with 5 and 10 oocysts were 25.0 and 75.0%, respectively, with the real-time PCR assay and 18.8 and 68.8%, respectively, with the nested PCR assay. All unseeded samples were negative, and all samples seeded with 200 oocysts were positive. Detection rates using the two PCR methods were statistically similar, but the real-time PCR assay is less laborious and less prone to amplicon contamination and allows monitoring of amplification and analysis of results, making it more attractive to diagnostic testing laboratories. The improved sample preparation steps and the TaqMan real-time PCR assay provide a robust, streamlined, and rapid analytical procedure for surveillance, outbreak response, and regulatory testing of foods for detection of C. cayetanensis.
[Mh] MeSH terms primary: Cyclospora/isolation & purification
Food Contamination/analysis
Real-Time Polymerase Chain Reaction/methods
United States Food and Drug Administration
[Mh] MeSH terms secundary: Animals
Cyclosporiasis
Humans
Oocysts
United States
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1710
[Cu] Class update date: 171016
[Lr] Last revision date:171016
[Js] Journal subset:IM
[Da] Date of entry for processing:170608
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-492


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