Database : MEDLINE
Search on : Ephemeral and Fever [Words]
References found : 267 [refine]
Displaying: 1 .. 10   in format [Detailed]

page 1 of 27 go to page                         

  1 / 267 MEDLINE  
              next record last record
select
to print
Photocopy
Full text

[PMID]: 29301517
[Au] Autor:Hou P; Zhao G; He C; Wang H; He H
[Ad] Address:Key Laboratory of Animal Resistant Biology of Shandong, Ruminant Disease Research Center, College of Life Sciences, Shandong Normal University, No. 88 East Wenhua Road, Jinan City, Shandong Province, China.
[Ti] Title:Biopanning of polypeptides binding to bovine ephemeral fever virus G protein from phage display peptide library.
[So] Source:BMC Vet Res;14(1):3, 2018 Jan 04.
[Is] ISSN:1746-6148
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: The bovine ephemeral fever virus (BEFV) glycoprotein neutralization site 1 (also referred as G protein), is a critical protein responsible for virus infectivity and eliciting immune-protection, however, binding peptides of BEFV G protein are still unclear. Thus, the aim of the present study was to screen specific polypeptides, which bind BEFV G protein with high-affinity and inhibit BEFV replication. METHODS: The purified BEFV G was coated and then reacted with the M13-based Ph.D.-7 phage random display library. The peptides for target binding were automated sequenced after four rounds of enrichment biopanning. The amino acid sequences of polypeptide displayed on positive clones were deduced and the affinity of positive polypeptides with BEFV G was assayed by ELISA. Then the roles of specific G -binding peptides in the context of BEFV infection were analyzed. RESULTS: The results showed that 27 specific peptide ligands displaying 11 different amino acid sequences were obtained, and the T18 and T25 clone had a higher affinity to G protein than the other clones. Then their antiviral roles of two phage clones (T25 and T18) showed that both phage polypeptide T25 and T18 exerted inhibition on BEFV replication compared to control group. Moreover, synthetic peptide based on T18 (HSIRYDF) and T25 (YSLRSDY) alone or combined use on BEFV replication showed that the synthetic peptides could effectively inhibit the formation of cytopathic plaque and significantly inhibit BEFV RNA replication in a dose-dependent manner. CONCLUSION: Two antiviral peptide ligands binding to bovine ephemeral fever virus G protein from phage display peptide library were identified, which may provide a potential research tool for diagnostic reagents and novel antiviral agents.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180210
[Lr] Last revision date:180210
[St] Status:In-Process
[do] DOI:10.1186/s12917-017-1315-x

  2 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 29288049
[Au] Autor:Hou P; Zhao G; Wang H; He C; Huan Y; He H
[Ad] Address:Key Laboratory of Animal Resistant Biology of Shandong, Ruminant Diseases Research Center, College of Life Sciences, Shandong Normal University, Jinan, China. Electronic address: apeilihou@163.com.
[Ti] Title:Development of a recombinase polymerase amplification combined with lateral-flow dipstick assay for detection of bovine ephemeral fever virus.
[So] Source:Mol Cell Probes;, 2017 Dec 26.
[Is] ISSN:1096-1194
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Bovine ephemeral fever virus (BEFV), identified as the causative pathogen of bovine ephemeral fever (BEF), is responsible for increasing numbers of epidemics/outbreaks and has a significant harmful effect on the livestock industry. Therefore, a rapid detection assay is imperative for BEFV diagnosis. In this study, we described the development of lateral-flow dipstick isothermal recombinase polymerase amplification (LFD-RPA) assays for detection of BEFV. RPA primers and LF probes were designed by targeting the specific G gene, and the amplification product can be visualized on a simple lateral flow dipstick with the naked eyes. The amplification reaction was performed at 38 °C for 20 min and LFD incubation time within 5 min. The detection limit of this assay was 8 copies per reaction, and there was no cross-reactivity with other bovine infectious viruses such as bovine viral diarrhea virus, infectious bovine rhinotracheitis virus, bovine respiratory syncytial virus, bovine coronavirus, bovine parainfluenza virus type 3, bovine vesicular stomatitis virus. In addition, the assay was performed with total 128 clinical specimens and the diagnostic results were compared with conventional RT-PCR, real-time quantative(q) PCR. The result showed that the coincidence rate of BEFV LFD-RPA and real-time qPCR was 96.09% (123/128), which was higher than conventional RT-PCR. The RPA combined with LFD assay probably provides a rapid and sensitive alternative for diagnosis of BEFV infections outbreak.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180107
[Lr] Last revision date:180107
[St] Status:Publisher

  3 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 29085608
[Au] Autor:Yazdani F; Bakhshesh M; Esmaelizad M; Sadigh ZA
[Ad] Address:Department of Animal Virology, Research and Diagnosis, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Organization Extension (AREEO), Karaj, Iran.
[Ti] Title:Expression of G1- epitope of bovine ephemeral fever virus in : A novel candidate to develop ELISA kit.
[So] Source:Vet Res Forum;8(3):209-213, 2017.
[Is] ISSN:2008-8140
[Cp] Country of publication:Iran
[La] Language:eng
[Ab] Abstract:Bovine ephemeral fever is an acute and arthropod-borne viral disease of cattle and water buffalo which occurs seasonally in most of the world tropical and subtropical regions. The epizootic feature of the disease has been reported in Iran with serious economic consequences. The surface glycoprotein G of bovine ephemeral fever virus (BEFV) is composed of 4 antigenic sites (G1-G4) and plays the main role for eliciting neutralizing antibodies and protective immunity. The G1 - epitope is a linear antigenic site and conserved among BEFV strains. In order to develop an ELISA test based on G1-epitope as coating antigen, this study was carried out to express the recombinant G1-epitope of BEFV in prokaryotic system. Using PCR and specific primers, a length of 88 amino acid of the G glycoprotein of BEFV including G1- epitope was amplified and cloned into the expression vector pGEX-4T-1, with the GST moiety. The recombinant plasmid (pGEX-4T-1-G1) was then transformed into BL and expression of fusion protein was induced by 0.10 mM IPTG. The maximum expression of the fusion protein was obtained at 16 hr post induction as verified by SDS-PAGE electrophoresis, and it was also confirmed that this protein bearing G1- epitope is sufficiently biologically active to bind to anti-BEFV serum in western blot experiment.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1711
[Cu] Class update date: 171102
[Lr] Last revision date:171102
[St] Status:PubMed-not-MEDLINE

  4 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28808793
[Au] Autor:Gao S; Du J; Tian Z; Niu Q; Zheng F; Huang D; Kang B; Luo J; Liu G; Yin H
[Ad] Address:State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, 730046, Gansu, People's Republic of China.
[Ti] Title:Complete genome sequence of a bovine ephemeral fever virus JT02L strain in mainland China.
[So] Source:Arch Virol;162(11):3555-3558, 2017 Nov.
[Is] ISSN:1432-8798
[Cp] Country of publication:Austria
[La] Language:eng
[Ab] Abstract:In this study, we report the complete genome sequence of bovine ephemeral fever virus (BEFV) JT02L, which has been used in our laboratory, in mainland China, for more than a decade. The genome is 14941 nucleotide (nt), comprising a leader sequence of 50 nt, nucleoprotein (N) gene of 1328 nt, phosphoprotein (P) gene of 858 nt, matrix protein (M) gene of 691 nt, glycoprotein (G) gene of 1897 nt, non-structural glycoprotein (G ) gene of 1785 nt, α1α2 gene of 638 nt, ß gene of 460 nt, γ gene of 400 nt, large multi-functional enzyme (L) gene of 6470 nt and a trailer sequence of 73 nt. Individual genes are separated by intergenic regions (IGRs) of 26, 44, 47, 51, 37, 39, 68 and -21 nt respectively. The overall organization is similar to an Australian BEFV isolate BB7721 but demonstrates some distinctive features including longer α3 and ß open reading frames, intact termination/polyadenylation (TTP) sequence downstream of the ß open reading frame and a longer ß-γ IGR integrated with a 38 nt AT-rich fragment. To our knowledge, this is the first report describing the complete genome of a BEFV strain of East Asian lineage, which may facilitate studies on genomic diversity among geographic strains of BEFV in China and the world.
[Mh] MeSH terms primary: Ephemeral Fever Virus, Bovine/genetics
Ephemeral Fever/virology
Genome, Viral
[Mh] MeSH terms secundary: Animals
Base Sequence
Cattle
China/epidemiology
Ephemeral Fever/epidemiology
Phylogeny
RNA, Viral/genetics
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (RNA, Viral)
[Em] Entry month:1710
[Cu] Class update date: 171025
[Lr] Last revision date:171025
[Js] Journal subset:IM
[Da] Date of entry for processing:170816
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3520-0

  5 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28674863
[Au] Autor:Abayli H; Tonbak S; Azkur AK; Bulut H
[Ad] Address:Department of Virology, Faculty of Veterinary Medicine, Firat University, 23110, Elazig, Turkey.
[Ti] Title:Complete genome analysis of highly pathogenic bovine ephemeral fever virus isolated in Turkey in 2012.
[So] Source:Arch Virol;, 2017 Jul 03.
[Is] ISSN:1432-8798
[Cp] Country of publication:Austria
[La] Language:eng
[Ab] Abstract:Relatively high prevalence and mortality rates of bovine ephemeral fever (BEF) have been reported in recent epidemics in some countries, including Turkey, when compared with previous outbreaks. A limited number of complete genome sequences of BEF virus (BEFV) are available in the GenBank Database. In this study, the complete genome of highly pathogenic BEFV isolated during an outbreak in Turkey in 2012 was analyzed for genetic characterization. The complete genome of the Turkish BEFV isolate was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced. It was found that the complete genome of the Turkish BEFV isolate was 14,901 nt in length. The complete genome sequence obtained from the study showed 91-92% identity at nucleotide level to Australian (BB7721) and Chinese (Bovine/China/Henan1/2012) BEFV isolates. Phylogenetic analysis of the glycoprotein gene of the Turkish BEFV isolate also showed that Turkish isolates were closely related to Israeli isolates. Because of the limited number of complete BEFV genome sequences, the results from this study will be useful for understanding the global molecular epidemiology and geodynamics of BEF.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1707
[Cu] Class update date: 170704
[Lr] Last revision date:170704
[St] Status:Publisher
[do] DOI:10.1007/s00705-017-3470-6

  6 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28392506
[Au] Autor:Hirashima Y; Nojiri M; Ohtsuka Y; Kato T; Shirafuji H; Kurazono M; Imafuji T; Yanase T
[Ad] Address:Kagoshima Central Livestock Hygiene Service Center, 1678 Yuda, Higashiichiki, Hioki, Kagoshima 899-2201, Japan.
[Ti] Title:Resurgence of bovine ephemeral fever in mainland Japan in 2015 after a 23-year absence.
[So] Source:J Vet Med Sci;79(5):904-911, 2017 May 18.
[Is] ISSN:1347-7439
[Cp] Country of publication:Japan
[La] Language:eng
[Ab] Abstract:In September and October 2015, suspected cases of bovine ephemeral fever (BEF) were reported in the mainland region of Kagoshima Prefecture and on Tanegashima Island. The genome of the BEF virus (BEFV) was detected in the diseased cows and the cows that had recovered. The serum obtained from the affected cows contained high titers of BEFV-neutralizing antibody. In total, 18 affected cows were demonstrated to be infected with BEFV during the outbreak. Our findings showed evidence that BEF occurred in mainland Japan after a 23-year absence. Phylogenetic analysis based on the surface glycoprotein (G) gene revealed that BEFVs detected in the affected cows were genetically distinct from previous Japanese BEFVs, but were close to BEFVs circulating in Taiwan and mainland China in recent years. Amino acid substitution in the neutralizing epitope domains of the G protein was limited between the detected viruses and the vaccine strain (YHL isolate), and high titers of the neutralizing antibody against the YHL isolate were induced in the infected cattle during the disease occurrences. Therefore, current BEF vaccines probably elicit protective immunity against the BEFVs detected in 2015, although their effectiveness should be assessed. Since the BEFV vaccination rates are estimated to be low, a BEF outbreak should be considered a possibility in mainland Japan.
[Mh] MeSH terms primary: Ephemeral Fever/diagnosis
[Mh] MeSH terms secundary: Animals
Antibodies, Neutralizing/blood
Antibodies, Viral/blood
Cattle
Disease Outbreaks/prevention & control
Disease Outbreaks/veterinary
Ephemeral Fever/epidemiology
Ephemeral Fever/prevention & control
Ephemeral Fever/virology
Ephemeral Fever Virus, Bovine/classification
Ephemeral Fever Virus, Bovine/genetics
Ephemeral Fever Virus, Bovine/isolation & purification
Female
Insect Vectors/virology
Japan/epidemiology
Phylogeny
Viral Vaccines/administration & dosage
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Viral Vaccines)
[Em] Entry month:1710
[Cu] Class update date: 171031
[Lr] Last revision date:171031
[Js] Journal subset:IM
[Da] Date of entry for processing:170411
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0345

  7 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28124418
[Au] Autor:Barigye R; Davis S; Hunt R; Hunt N; Walsh S; Elliott N; Dyrting K; Weir R; Melville LF
[Ad] Address:Berrimah Veterinary Laboratories, Department of Primary Industry & Fisheries, Darwin, Northern Territory, Australia.
[Ti] Title:Post-viraemic detection of bovine ephemeral fever virus by use of autogenous lymphoid tissue-derived bovine primary cell cultures.
[So] Source:Aust Vet J;95(1-2):49-52, 2017 Jan.
[Is] ISSN:1751-0813
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: The potential tissue replication sites and specific cell types that support in vivo virus survival beyond the acute phase of bovine ephemeral fever virus (BEFV) infection have not been fully defined in cattle. To clarify the knowledge gap, tissue specimens were tested after collection from an adult steer necropsied 1 week after acute BEF. CASE REPORT: Significant necropsy findings included fibrinoproliferative synovitis in the stifle joints and fibrin clot-laden fluid in serous body cavities. Moderate numbers of infiltrating neutrophils were demonstrated in sections of the prefemoral lymph nodes and haemal node, and lymphoid hyperplasia in the spleen, haemal node and prefemoral lymph nodes. Viral RNA was detected by qRT-PCR in fresh spleen, haemal node, prefemoral lymph node, synovial fluid and in several spleen-derived cell cultures. BEFV was isolated from autogenously derived splenic primary cell cultures 6 days after cessation of viraemia, and characteristic bullet-shaped virions were confirmed by electron microscopy of an ultrathin haemal node section. In sections of the spleen, haemal node and other tissues, immunohistochemistry demonstrated BEFV antigens that were intracellularly associated with probable histiocytic cells. CONCLUSION: BEFV has preferential tropism for bovine lymphoid tissues and the spleen and haemal node may be potential sites for post-viraemic virus replication.
[Pt] Publication type:CASE REPORTS
[Em] Entry month:1701
[Cu] Class update date: 170126
[Lr] Last revision date:170126
[St] Status:In-Process
[do] DOI:10.1111/avj.12551

  8 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 28124421
[Au] Autor:Jackson AE
[Ti] Title:In this issue - January/February 2017: Experimental Hendra virus infection of dogs · Brucella suis in dogs · Cytokines in stored packed red blood cells · Transitional lumbosacral vertebrae in Labradors · Chemotherapy for histiocytic sarcomas · Pyothorax and oesophageal foreign bodies · Osteochondroma in a horse · Detection of bovine ephemeral fever virus.
[So] Source:Aust Vet J;95(1-2):1-3, 2017 Jan.
[Is] ISSN:1751-0813
[Cp] Country of publication:England
[La] Language:eng
[Pt] Publication type:EDITORIAL
[Em] Entry month:1701
[Cu] Class update date: 170126
[Lr] Last revision date:170126
[St] Status:In-Process
[do] DOI:10.1111/avj.12555

  9 / 267 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 27916572
[Au] Autor:Erster O; Stram R; Menasherow S; Rubistein-Giuni M; Sharir B; Kchinich E; Stram Y
[Ad] Address:Molecular Virology Unit, Virology Division, Kimron Veterinary Institute, Bet-Dagan, 50250, Israel.
[Ti] Title:High-resolution melting (HRM) for genotyping bovine ephemeral fever virus (BEFV).
[So] Source:Virus Res;229:1-8, 2017 Feb 02.
[Is] ISSN:1872-7492
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:In recent years there have been several major outbreaks of bovine ephemeral disease in the Middle East, including Israel. Such occurrences raise the need for quick identification of the viruses responsible for the outbreaks, in order to rapidly identify the entry of viruses that do not belong to the Middle-East BEFV lineage. This challenge was met by the development of a high-resolution melt (HRM) assay. The assay is based on the viral G gene sequence and generation of an algorithm that calculates and evaluates the GC content of various fragments. The algorithm was designed to scan 50- to 200-base-long segments in a sliding-window manner, compare and rank them using an Order of Technique of Preference by Similarity to Ideal Solution (TOPSIS) the technique for order preference by similarity to ideal solution technique, according to the differences in GC content of homologous fragments. Two fragments were selected, based on a match to the analysis criteria, in terms of size and GC content. These fragments were successfully used in the analysis to differentiate between different virus lineages, thus facilitating assignment of the viruses' geographical origins. Moreover, the assay could be used for differentiating infected from vaccinated animales (DIVA). The new algorithm may therefore be useful for development of improved genotyping studies for other viruses and possibly other microorganisms.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1612
[Cu] Class update date: 170115
[Lr] Last revision date:170115
[St] Status:In-Process

  10 / 267 MEDLINE  
              first record previous record
select
to print
Photocopy
Full text

[PMID]: 27757685
[Au] Autor:Zhang M; Ge J; Wen Z; Chen W; Wang X; Liu R; Bu Z
[Ad] Address:State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, People's Republic of China.
[Ti] Title:Characterization of a recombinant Newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus.
[So] Source:Arch Virol;162(2):359-367, 2017 Feb.
[Is] ISSN:1432-8798
[Cp] Country of publication:Austria
[La] Language:eng
[Ab] Abstract:Bovine ephemeral fever (BEF) is caused by the arthropod-borne bovine ephemeral fever virus (BEFV), which is a member of the family Rhabdoviridae and the genus Ephemerovirus. BEFV causes an acute febrile infection in cattle and water buffalo. In this study, a recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of BEFV (rL-BEFV-G) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. BEFV G enabled NDV to spread from cell to cell. rL-BEFV-G remained nonvirulent in poultry and mice compared with vector LaSota virus. rL-BEFV-G triggered a high titer of neutralizing antibodies against BEFV in mice and cattle. These results suggest that rL-BEFV-G might be a suitable candidate vaccine against BEF.
[Mh] MeSH terms primary: Antibodies, Neutralizing/biosynthesis
Antibodies, Viral/biosynthesis
Ephemeral Fever Virus, Bovine/genetics
Ephemeral Fever/prevention & control
Newcastle disease virus/genetics
Viral Vaccines/genetics
[Mh] MeSH terms secundary: Animals
Cattle
Chick Embryo
Cricetinae
Dogs
Ephemeral Fever/immunology
Ephemeral Fever/virology
Ephemeral Fever Virus, Bovine/drug effects
Ephemeral Fever Virus, Bovine/immunology
Epithelial Cells/virology
Gene Expression
Genetic Vectors/chemistry
Genetic Vectors/immunology
Glycoproteins/administration & dosage
Glycoproteins/genetics
Glycoproteins/immunology
Immunization
Madin Darby Canine Kidney Cells
Mice
Mice, Inbred BALB C
Newcastle disease virus/immunology
Reassortant Viruses/genetics
Reassortant Viruses/immunology
Vaccines, Synthetic
Viral Proteins/administration & dosage
Viral Proteins/genetics
Viral Proteins/immunology
Viral Vaccines/administration & dosage
Viral Vaccines/immunology
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antibodies, Neutralizing); 0 (Antibodies, Viral); 0 (Glycoproteins); 0 (Vaccines, Synthetic); 0 (Viral Proteins); 0 (Viral Vaccines)
[Em] Entry month:1702
[Cu] Class update date: 170226
[Lr] Last revision date:170226
[Js] Journal subset:IM
[Da] Date of entry for processing:161021
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3078-2


page 1 of 27 go to page                         
   


Refine the search
  Database : MEDLINE Advanced form   

    Search in field  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/PAHO/WHO - Latin American and Caribbean Center on Health Sciences Information