Database : MEDLINE Search on : Femoral and Artery [Words] References found : 33939 [refine] Displaying: 1 .. 10   in format [Detailed]

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[PMID]: 23537403 [Au] Autor: Karimi A; de Boer SW; van den Heuvel DA; Fioole B; Vroegindeweij D; Heyligers JM; Lohle PN; Elgersma O; Nolthenius RP; Vos JA; de Vries JP [Ad] Address: Department of Vascular Surgery, St, Antonius Hospital, PO box 2500, Nieuwegein, EM 3430, The Netherlands. a.karimi@antoniusziekenhuis.nl. [Ti] Title: Randomized trial of Legflow® paclitaxel eluting balloon and stenting versus standard percutaneous transluminal angioplasty and stenting for the treatment of intermediate and long lesions of the superficial femoral artery (RAPID trial): study protocol for a randomized controlled trial. [So] Source: Trials;14(1):87, 2013. [Is] ISSN: 1745-6215 [Cp] Country of publication: England [La] Language: eng [Ab] Abstract: BACKGROUND: Restenosis after percutaneous transluminal angioplasty (PTA) of the superficial femoral artery (SFA) may occur in 45% of patients at 2 years follow-up. Paclitaxel-coated balloons have been found to reduce neointimal hyperplasia, and thus reduce restenosis. Recently, the Legflow® paclitaxel-coated balloon (Cardionovum Sp.z.o.o., Warsaw, Poland) (LPEB) has been introduced. This balloon is covered with shellac, a Food and Drug Administration (FDA) approved natural resin, to obtain an equally distributed tissue concentration of paclitaxel. The RAPID trial is designed to assess restenosis after PTA using the Legflow balloon combined with nitinol stenting versus uncoated balloons with nitinol stenting in SFA lesions >5 cm. METHODS/DESIGN: A total of 176 adult patients with Rutherford class 2 to class 6 symptoms due to intermediate (5-15 cm) or long (>15 cm) atherosclerotic lesions in the SFA will be randomly allocated for treatment with LPEB with nitinol stenting or uncoated balloon angioplasty with stenting. Stenting will be performed using the Supera® stent in both groups (IDEV Technologies Inc., Webster, TX). The primary endpoint is the absence of binary restenosis of the treated SFA segment. Secondary outcomes are target lesion revascularization (TLR), clinical and hemodynamic outcome, amputation rate, mortality rate, adverse events, and device-specific adverse events. Follow up consists of four visits in which ankle-brachial indices (ABI), toe pressure measurements, and duplex ultrasound (DUS) will be performed. Furthermore, a peripheral artery questionnaire (PAQ) will be completed by the patients at each follow-up. In the event that DUS reveals a symptomatic >50% restenosis, or a >75% asymptomatic restenosis, additional digital subtraction angiography will be performed with any necessary re-intervention. DISCUSSION: The RAPID trial is a multicenter randomized controlled patient blind trial that will provide evidence concerning whether the use of the Legflow paclitaxel/shellac coated balloons with nitinol stenting significantly reduces the frequency of restenosis in intermediate and long SFA lesions compared to standard PTA and stenting. TRIAL REGISTRATION: ISRCTN47846578. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1305 [Js] Journal subset: IM [St] Status: In-Data-Review [do] DOI: 10.1186/1745-6215-14-87

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[PMID]: 23667791 [Au] Autor: Liang CP; Wu Y; Schmitt J; Bigeleisen PE; Slavin J; Jafri MS; Tang CM; Chen Y [Ad] Address: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA. [Ti] Title: Coherence-gated Doppler: a fiber sensor for precise localization of blood flow. [So] Source: Biomed Opt Express;4(5):760-71, 2013 May 1. [Is] ISSN: 2156-7085 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Miniature optical sensors that can detect blood vessels in front of advancing instruments will significantly benefit many interventional procedures. Towards this end, we developed a thin and flexible coherence-gated Doppler (CGD) fiber probe (O.D. = 0.125 mm) that can be integrated with minimally-invasive tools to provide real-time audio feedback of blood flow at precise locations in front of the probe. Coherence-gated Doppler (CGD) is a hybrid technology with features of laser Doppler flowmetry (LDF) and Doppler optical coherence tomography (DOCT). Because of its confocal optical design and coherence-gating capabilities, CGD provides higher spatial resolution than LDF. And compared to DOCT imaging systems, CGD is simpler and less costly to produce. In vivo studies of rat femoral vessels using CGD demonstrate its ability to distinguish between artery, vein and bulk movement of the surrounding soft tissue. Finally, by placing the CGD probe inside a 30-gauge needle and advancing it into the brain of an anesthetized sheep, we demonstrate that it is capable of detecting vessels in front of advancing probes during simulated stereotactic neurosurgical procedures. Using simultaneous ultrasound (US) monitoring from the surface of the brain we show that CGD can detect at-risk blood vessels up to 3 mm in front of the advancing probe. The improved spatial resolution afforded by coherence gating combined with the simplicity, minute size and robustness of the CGD probe suggest it may benefit many minimally invasive procedures and enable it to be embedded into a variety of surgical instruments. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1305 [Da] Date of entry for processing: 130513 [St] Status: PubMed-not-MEDLINE [do] DOI: 10.1364/BOE.4.000760

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[PMID]: 23658704 [Au] Autor: Zhang JM; Wang Y; Miao YJ; Zhang Y; Wu YN; Jia LX; Qi YF; Du J [Ad] Address: Beijing An Zhen Hospital, Capital Medical University; The Key Laboratory of Remodeling-related Cardiovascular Diseases, Ministry of Education, Institute of Heart Lung and Blood Vessel Diseases, Beijing, China. [Ti] Title: Knockout of CD8 Delays Reendothelialization and Accelerates Neointima Formation in Injured Arteries of Mouse via TNF-α Inhibiting the Endothelial Cells Migration. [So] Source: PLoS One;8(5):e62001, 2013. [Is] ISSN: 1932-6203 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: OBJECTIVE: Delayed or impaired reendothelialization is a major cause of stent thrombosis in the interventional treatment of coronary heart disease. T cells are involved in neointima formation of injured arteries. However, the regulated mechanism of reendothelialization and the role of CD8 T cell in reendothelialization are unclear. METHODS AND RESULTS: Immunofluorescence staining showed that CD8 positive cells were increased in wire injured femoral artery of mice. On day 21 after injury, elastin staining showed that knockout of CD8 (CD8(-/-)) significantly increased intimal thickness and a ratio of intima to media by 1.8 folds and 1.9 folds respectively in injured arteries. Evans blue staining showed that knockout of CD8 delayed the reendothelialization area on day 7 after injury (18.8±0.5% versus 42.1±5.6%, p<0.05). In vitro, a migration assay revealed that CD8(-/-) T cells co-cultured with WT macrophages significantly inhibited the migration of the endothelial cells (ECs); compared to CD4(+) T cells, and CD8(+) T cells could promote the ECs migration. Furthermore, real-time PCR analysis showed that knockout of CD8 increased the level of tumor necrosis factor α (TNF-α) in injured arteries and cytometric bead cytokine array showed that TNF-α was elevated in cultured CD8(-/-) T cells. Finally, a wound-healing assay showed that recombinant TNF-α significantly inhibited the migration of ECs. CONCLUSION: Our study suggested that CD8(+) T cells could promote the reendothelialization and inhibit the neointima formation after the artery wire injury, and this effect is at least partly dependent on decreasing TNF-α production promoting ECs migration. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1305 [Js] Journal subset: IM [St] Status: In-Data-Review [do] DOI: 10.1371/journal.pone.0062001

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[PMID]: 23076403 [Au] Autor: Gurovich AN; Braith RW [Ad] Address: Center for Exercise Science, Department of Applied Physiology and Kinesiology, College of Health and Human Performance, University of Florida, Gainesville, FL, USA. Alvaro.Gurovich@indstate.edu [Ti] Title: Enhanced external counterpulsation creates acute blood flow patterns responsible for improved flow-mediated dilation in humans. [So] Source: Hypertens Res;36(4):297-305, 2013 Apr. [Is] ISSN: 1348-4214 [Cp] Country of publication: England [La] Language: eng [Ab] Abstract: Enhanced external counterpulsation (EECP) is a FDA-approved treatment for patients with coronary artery disease and unstable angina. Although beneficial effects of EECP have been linked to central/cardiac adaptations, recent findings have shown peripheral/vascular effects. Here, we sought to determine EECP-induced blood flow patterns and their association with vascular function. The present study was designed to investigate endothelium-mediated arterial vasodilation changes after one 45-min session of either EECP or Sham EECP in 18 randomly assigned apparently healthy, young men (25±4 years). Brachial (b) and femoral (f) flow-mediated dilation (FMD) were assessed before and within 10 min after completing EECP or Sham. After 20 min of EECP, peak blood flow velocity (V) and brachial and femoral artery diameters (D) were recorded live for 2 min. In addition, a blood sample was drawn from the earlobe to determine hematocrit and then to calculate blood viscosity (µ) and density (Ï�), Reynolds number (Re=V*D*Ï�/µ), and endothelial shear stress (ESS=2µ*V/D). EECP increased retrograde shear stress and retrograde-turbulent blood flow in the femoral artery and antegrade-laminar shear stress in the brachial artery. fFMD was increased after EECP compared with Sham and baseline (fFMD=13.1±3.7 vs. 7.9±4.6% and 7.8±4.5%, respectively, P<0.05) and bFMD was increased after EECP compared with baseline (bFMD=10.6±4.8 vs. 7.0±3.5%, P<0.05), despite different blood flow patterns. These results provide novel evidence that a single session of EECP-induced blood flow patterns improve endothelial function in peripheral muscular conduit arteries. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1304 [Js] Journal subset: IM [St] Status: In-Process [do] DOI: 10.1038/hr.2012.169

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[PMID]: 23557564 [Au] Autor: Lu G; Cui SJ; Geng X; Ye L; Chen B; Feng ZG; Zhang J; Li ZZ [Ad] Address: Department of Pediatric Cardiac Surgery, Beijing Children Hospital, Capital Medical University, Beijing 100045, China. [Ti] Title: Design and preparation of polyurethane-collagen/heparin-conjugated polycaprolactone double-layer bionic small-diameter vascular graft and its preliminary animal tests. [So] Source: Chin Med J (Engl);126(7):1310-6, 2013 Apr. [Is] ISSN: 0366-6999 [Cp] Country of publication: China [La] Language: eng [Ab] Abstract: BACKGROUND: People recently realized that it is important for artificial vascular biodegradable graft to bionically mimic the functions of the native vessel. In order to overcome the high risk of thrombosis and keep the patency in the clinical small-diameter vascular graft (SDVG) transplantation, a double-layer bionic scaffold, which can offer anticoagulation and mechanical strength simultaneously, was designed and fabricated via electrospinning technique. METHODS: Heparin-conjugated polycaprolactone (hPCL) and polyurethane (PU)-collagen type I composite was used as the inner and outer layers, respectively. The porosity and the burst pressure of SDVG were evaluated. Its biocompatibility was demonstrated by the 3-(4,5-dimethyl-2-thiazol)-2,5-diphenyl-2H tetrazolium bromide (MTT) test in vitro and subcutaneous implants in vivo respectively. The grafts of diameter 2.5 mm and length 4.0 cm were implanted to replace the femoral artery in Beagle dog model. Then, angiography was performed in the Beagle dogs to investigate the patency and aneurysm of grafts at 2, 4, and 8 weeks post-transplantation. After angiography, the patent grafts were explanted for histological analysis. RESULTS: The double-layer bionic SDVG meet the clinical mechanical demand. Its good biocompatibility was proven by cytotoxicity experiment (the cell's relative growth rates (RGR) of PU-collagen outer layer were 102.8%, 109.2% and 103.5%, while the RGR of hPCL inner layer were 99.0%, 100.0% and 98.0%, on days 1, 3, and 5, respectively) and the subdermal implants experiment in the Beagle dog. Arteriography showed that all the implanted SDVGs were patent without any aneurismal dilatation or obvious anastomotic stenosis at the 2nd, 4th, and 8th week after the operation, except one SDVG that failed at the 2nd week. Histological analysis and SEM showed that the inner layer was covered by new endothelial-like cells. CONCLUSION: The double-layer bionic SDVG is a promising candidate as a replacement of native small-diameter vascular graft. [Pt] Publication type: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Entry month: 1304 [Js] Journal subset: IM [St] Status: In-Process

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[PMID]: 23558988 [Au] Autor: Rodrigues FA; Chianca-Jr DA; Gonçalves Fernandes L [Ad] Address: Nucleus of Biological Research, Federal University of Ouro Preto UFOP, Ouro Preto, MG, Brazil. [Ti] Title: Malnutrition affects the pressor response to microinjection of L-glutamate into the RVLM of awake rats. [So] Source: Biol Res;45(4):337-43, 2012. [Is] ISSN: 0717-6287 [Cp] Country of publication: Chile [La] Language: eng [Ab] Abstract: Post-weaning protein malnutrition is often related to the development of cardiovascular and metabolic diseases in humans, as well to changed content of neurotransmitters in the central nervous system under experimental conditions. The rostral ventrolateral medulla (RVLM) is a bulbar region that contains sympathetic premotor neurons; the excitatory amino acid L-glutamate seems to be the main neurotransmitter at this level. The aim of the present study was to evaluate the possible change in the L-glutamate sensitivity of the RVLM neurons of malnourished animals. Male Fischer rats were divided into two groups: control (n = 15) and malnourished (n = 19). Four days before the experiments, guide cannulas were implanted bilaterally in direction of the RVLM for microinjection of L-glutamate. Twenty-four hours before the experiments, the femoral artery was cannulated for cardiovascular recordings. The results showed that the baseline heart rate increased in malnourished compared to control animals (412.18 ± 16.03 bpm vs. 370.74 ± 9.59 bpm, respectively). Malnourished animals presented a dissimilar concentration-dependent pressor response curve to L-glutamate and an attenuated baroreflex gain. Our results suggest that post-weaning protein restriction affects glutamatergic neurotransmission of the baroreflex at the RVLM level. [Pt] Publication type: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Entry month: 1304 [Js] Journal subset: IM [St] Status: In-Process

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[PMID]: 23536941 [Au] Autor: Noskov SM; Zavodchikov AA; Evgen'eva AV; Lavrukhina AA; Chamorovskii AN; Prokopenko ON; Poletaeva VS [Ti] Title: [Relationship of subclinical atherosclerosis indicators to exercise tolerance in patients with coronary heart disease]. [So] Source: Ter Arkh;85(1):20-4, 2013. [Is] ISSN: 0040-3660 [Cp] Country of publication: Russia (Federation) [La] Language: rus [Ab] Abstract: AIM: To study the spread of a number of indicators of subclinical atherosclerosis in patients with coronary heart disease (CHD) and the pattern of their relationship to their functional state and muscle mass. SUBJECTS AND METHODS: Twenty patients with chronic forms of CHD were examined. Vascular wall stiffness was judged from pulse wave velocity (PWV) along elastic-type vessels, by calculating the carotid-femoral index (CFI). Endothelial function was estimated by endothelium-dependent vasodilation (EDV) in a reactive hyperemia test. Ultrasound scanning of the common carotid artery was used to estimate intima-media thickness. All the patients underwent bicycle ergometry; their exercise tolerance (ET) was determined by the amount of consumed oxygen when the metabolic equivalent (MET) was calculated; the percentages of active cell mass (ACM%) and lean mass (LM%) of total body weight were determined; the levels of total cholesterol, high- and low-density lipoprotein cholesterol, and triglycerides were estimated. A control group comprised 20 matched persons without CHD. RESULTS: A prognostically unfavorable increase of >12 m/sec in CFI was revealed in 20% of the patients with CHD and in 10% of the controls; endothelial vasomotor dysfunction (EDV < 10%) in 65 and 50%; a more than 0.9-mm increase in 55 and 15%, respectively. A low ET was stated in the majority of patients with abnormal vascular stiffness and endothelial vasomotor dysfunction. The CHD patients with a low ET were found to have significant correlations of CFI with ACM% and LM%, EDV and ACM% and LM%, CFI and EDV. The control patients with a low ET also showed correlations of EDV and ACM% and LM%. There was a correlation of ACM% and LM% with MET in both groups. CONCLUSION: EDV and PWV reflect a more decrease in physical activity and dysadapted functional status than in the degree of subclinical atherosclerosis. [Mh] MeSH terms primary: Atherosclerosis/physiopathology Coronary Disease/physiopathology Exercise Tolerance/physiology Vascular Stiffness/physiology Vasodilation/physiology [Mh] MeSH terms secundary: Atherosclerosis/diagnosis Brachial Artery/physiopathology Brachial Artery/ultrasonography Carotid Arteries/physiopathology Carotid Arteries/ultrasonography Carotid Intima-Media Thickness Coronary Disease/diagnosis Female Humans Male Middle Aged [Pt] Publication type: COMPARATIVE STUDY; ENGLISH ABSTRACT; JOURNAL ARTICLE [Em] Entry month: 1305 [Js] Journal subset: IM [Da] Date of entry for processing: 130328 [St] Status: MEDLINE

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[PMID]: 23185266 [Au] Autor: Prisby RD; Dominguez JM; Muller-Delp J; Allen MR; Delp MD [Ad] Address: Department of Kinesiology and Applied Physiology, University of Delaware, Newark, DE, USA. [Ti] Title: Aging and estrogen status: a possible endothelium-dependent vascular coupling mechanism in bone remodeling. [So] Source: PLoS One;7(11):e48564, 2012. [Is] ISSN: 1932-6203 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Bone loss with aging and menopause may be linked to vascular endothelial dysfunction. The purpose of the study was to determine whether putative modifications in endothelium-dependent vasodilation of the principal nutrient artery (PNA) of the femur are associated with changes in trabecular bone volume (BV/TV) with altered estrogen status in young (6 mon) and old (24 mon) female Fischer-344 rats. Animals were divided into 6 groups: 1) young intact, 2) old intact, 3) young ovariectomized (OVX), 4) old OVX, 5) young OVX plus estrogen replacement (OVX+E2), and 6) old OVX+E2. PNA endothelium-dependent vasodilation was assessed in vitro using acetylcholine. Trabecular bone volume of the distal femoral metaphysis was determined by microCT. In young rats, vasodilation was diminished by OVX and restored with estrogen replacement (intact, 82±7; OVX, 61±9; OVX+E2, 90±4%), which corresponded with similar modifications in BV/TV (intact, 28.7±1.6; OVX, 16.3±0.9; OVX+E2, 25.7±1.4%). In old animals, vasodilation was unaffected by OVX but enhanced with estrogen replacement (intact, 55±8; OVX, 59±7; OVX+E2, 92±4%). Likewise, modifications in BV/TV followed the same pattern (intact, 33.1±1.6; OVX, 34.4±3.7; OVX+E2, 42.4±2.1%). Furthermore, in old animals with low endogenous estrogen (i.e., intact and old OVX), vasodilation was correlated with BV/TV (R(2) = 0.630; P<0.001). These data demonstrate parallel effects of estrogen on vascular endothelial function and BV/TV, and provide for a possible coupling mechanism linking endothelium-dependent vasodilation to bone remodeling. [Mh] MeSH terms primary: Aging/metabolism Bone Remodeling/physiology Endothelium, Vascular/physiology Estrogens/metabolism [Mh] MeSH terms secundary: Aging/blood Animals Arteries/drug effects Arteries/physiology Bone Remodeling/drug effects Bone and Bones/drug effects Bone and Bones/physiology Cyclooxygenase Inhibitors/pharmacology Endothelium, Vascular/drug effects Enzyme Inhibitors/pharmacology Estrogen Replacement Therapy Estrogens/blood Female Hydrazines/pharmacology NG-Nitroarginine Methyl Ester/pharmacology Nitric Oxide Synthase/antagonists & inhibitors Nitric Oxide Synthase/metabolism Organ Size/drug effects Ovariectomy Rats Rats, Inbred F344 Regression Analysis Vasodilator Agents/pharmacology [Pt] Publication type: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S. [Nm] Name of substance: 0 (Cyclooxygenase Inhibitors); 0 (Enzyme Inhibitors); 0 (Estrogens); 0 (Hydrazines); 0 (Vasodilator Agents); 50903-99-6 (NG-Nitroarginine Methyl Ester); 86831-65-4 (1,1-diethyl-2-hydroxy-2-nitrosohydrazine); EC 1.14.13.39 (Nitric Oxide Synthase) [Em] Entry month: 1305 [Js] Journal subset: IM [Da] Date of entry for processing: 121127 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0048564

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[PMID]: 22874889 [Au] Autor: Girerd N; Legedz L; Paget V; Rabilloud M; Milon H; Bricca G; Lantelme P [Ad] Address: Department of Cardiology, ESH Hypertension Excellence Center, Croix-Rousse Hospital, Hospices Civils de Lyon, France. nicolas_girerd@yahoo.com [Ti] Title: Outcome associations of carotid-femoral pulse wave velocity vary with different measurement methods. [So] Source: Am J Hypertens;25(12):1264-70, 2012 Dec. [Is] ISSN: 1941-7225 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: BACKGROUND: The impact of various methods of travel distance estimation on the prognostic value of pulse wave velocity (PWV) and on the adequacy of cut-offs has never been addressed within a single population of hypertensive patients. METHODS: Four carotid-femoral PWVs were calculated from four different travel distances (Direct, Real, Subtracted, and Estimated) divided by the same travel time in 426 hypertensives (mean age 51.2 ± 13.8 years, mean systolic blood pressure 155.6 ± 21.1 mm Hg). The incidence of death from any cause and major cardiovascular events was studied. PWV predictive accuracies were determined using C-index analysis. Hazard ratios (HRs) associated with specific values of PWV were determined with Cox model analyses using cubic splines. RESULTS: Mean PWV ranged from 8.3 ± 2.3 m/s for the Subtracted one to 11.6 ± 3.0 m/s for the Direct one (P < 0.001). When included as continuous variables in a Cox model, the four PWVs were significantly associated with outcome (all P < 0.001), and had similar C-index (0.608-0.617). In multivariable analysis, the HR calculated for a Direct PWV of 12 m/s was neutral (HR = 1.02). In contrast, the same analysis provided HR ranging from 1.79 to 2.90 with the other PWVs. CONCLUSIONS: Different travel distances markedly impact PWV values and prognostic cut-offs. PWV cut-offs should consequently be ascertained jointly with the method of measurement used. There is an urgent need for standardization of PWV assessment before implementing this parameter in the routine management of hypertensives. [Mh] MeSH terms primary: Arterial Pressure Carotid Arteries/physiopathology Femoral Artery/physiopathology Hypertension/diagnosis Pulse Wave Analysis/methods Pulse [Mh] MeSH terms secundary: Adult Aged Cause of Death Female Humans Hypertension/mortality Hypertension/physiopathology Kaplan-Meier Estimate Male Middle Aged Multivariate Analysis Predictive Value of Tests Prognosis Proportional Hazards Models Pulse Wave Analysis/standards Risk Factors [Pt] Publication type: COMPARATIVE STUDY; JOURNAL ARTICLE [Em] Entry month: 1305 [Js] Journal subset: IM [Da] Date of entry for processing: 121115 [St] Status: MEDLINE [do] DOI: 10.1038/ajh.2012.114

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[PMID]: 22445924 [Au] Autor: Song M; Shen HT; Cui JJ; Zhou XG; Zhong X; Peng CH; Liu HY; Tian Y [Ad] Address: Department of Cardiology, First Affiliated Hospital, Harbin Medical University, Harbin, 150001, Heilongjiang, China. [Ti] Title: A novel model of intimal hyperplasia with graded hypoosmotic damage. [So] Source: Cardiovasc Pathol;21(6):490-8, 2012 Nov-Dec. [Is] ISSN: 1879-1336 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: BACKGROUND: The purpose was to develop a rabbit model of intimal hyperplasia with controllable lesion. METHODS: Following 1 week of a 2% cholesterol diet, 32 New Zealand White male rabbits underwent right femoral arteries surgical perfusion with distilled water for 1, 3, 5, or 7 min (n=8/group). After a further 4 weeks of the same diet, serum total cholesterol, triglyceride, low-density lipoprotein, and high-density lipoprotein were measured in all rabbits. Intimal hyperplasia in histological sections of arteries were assessed by intimal proliferation ratio. Macrophage numbers and levels of proteins matrix metalloproteinase 9, tissue inhibitor of metalloproteinase 2, and alpha smooth muscle actin in lesions were analyzed by immunohistochemistry. RESULTS: Serum lipids levels showed no statistical difference between experimental groups. Intimal proliferation ratio increased gradually with perfusion time, and a positive linear correlation was calculated between intimal proliferation ratio and duration of distilled water perfusion. Similarly, number of macrophages and levels of matrix metalloproteinase 9, tissue inhibitor of metalloproteinase 2, and alpha smooth muscle actin in lesions increased with perfusion time. CONCLUSIONS: A novel model of intimal hyperplasia was established by intravascular distilled water perfusion in high-cholesterol-fed rabbits. Importantly, this model exhibits time-dependent neointimal proliferation lesions that can be readily controlled in terms of extent, thus providing an avenue for further studies. [Mh] MeSH terms primary: Diet, Atherogenic Disease Models, Animal Femoral Artery/pathology Tunica Intima/pathology [Mh] MeSH terms secundary: Actins/metabolism Animals Cell Count Cell Proliferation/drug effects Cholesterol, Dietary/adverse effects Femoral Artery/drug effects Hyperplasia/chemically induced Hyperplasia/pathology Macrophages/drug effects Macrophages/metabolism Macrophages/pathology Male Matrix Metalloproteinase 9/metabolism Osmotic Pressure/physiology Rabbits Time Factors Tissue Inhibitor of Metalloproteinase-2/metabolism Tunica Intima/drug effects [Pt] Publication type: JOURNAL ARTICLE [Nm] Name of substance: 0 (Actins); 0 (Cholesterol, Dietary); 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2); EC 3.4.24.35 (Matrix Metalloproteinase 9) [Em] Entry month: 1305 [Js] Journal subset: IM [Da] Date of entry for processing: 121109 [St] Status: MEDLINE

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