Database : MEDLINE
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[PMID]: 29339117
[Au] Autor:Feijoo-Siota L; Rama JLR; Sánchez-Pérez A; Villa TG
[Ad] Address:Department of Microbiology, Biotechnology Unit, University of Santiago de Compostela 15706, Spain.
[Ti] Title:Expression, activation and processing of a novel plant milk-clotting aspartic protease in Pichia pastoris.
[So] Source:J Biotechnol;268:28-39, 2018 Feb 20.
[Is] ISSN:1873-4863
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Galium verum, also known as Lady's Bedstraw or Cheese Rennet, is an herbaceous perennial plant traditionally used in cheese-making. We used RACE PCR to isolate novel enzymes from Galium verum with the ability to clot milk. This approach generated two cDNA sequences (named preprogaline A and B) encoding proteins displaying the typical plant aspartic protease primary structure. Preprogaline B was expressed in the yeast Pichia pastoris, after deleting and replacing its original signal peptide with the yeast α-factor signal peptide from Saccharomyces cerevisiae. The secreted recombinant protein was obtained by growing P. pastoris in YPD medium and had the ability to clot milk. The mature form of progaline B is a heterodimeric glycosylated enzyme, with a molecular weight of approximately 48 kDa, that contains a heavy (30.7 kDa) and a light (13.5 kDa) polypeptide chains linked by disulfide bonds. Western blot analysis revealed that progaline B is activated by the acidification of the yeast culture medium and that enzymatic activation requires two steps. First the precursor protein is cleaved into two polypeptide chains by partial removal of the plant-specific insert (PSI) present in plant aspartic proteases; this is later followed by propeptide removal. By altering the pH of the P. pastoris culture medium, we were able to obtain either active or inactive forms of the enzyme. Recombinant progaline B displayed a κ-casein hydrolysis pattern analogous to those produced by the animal and microbial coagulants currently used in the dairy industry, but it exhibited a different digestion profile on α- and ß-caseins. The plant protease progaline B displays milk-clotting activities suitable for the production of novel dairy products.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180206
[Lr] Last revision date:180206
[St] Status:In-Process

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[PMID]: 29305666
[Au] Autor:de Souza Albernaz M; Toma SH; Clanton J; Araki K; Santos-Oliveira R
[Ad] Address:Radiopharmacy Service, University Hospital Clementino Fraga Filho, Rio de Janeiro, Brazil.
[Ti] Title:Decorated Superparamagnetic Iron Oxide Nanoparticles with Monoclonal Antibody and Diethylene-Triamine-Pentaacetic Acid Labeled with Thechnetium-99m and Galium-68 for Breast Cancer Imaging.
[So] Source:Pharm Res;35(1):24, 2018 01 05.
[Is] ISSN:1573-904X
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PURPOSE: In this study we developed and tested an iron oxide nanoparticle conjugated with DTPA and Trastuzumab, which can efficiently be radiolabeled with 99m-Tc and Ga-68, generating a nanoradiopharmaceutical agent to be used for SPECT and PET imaging. METHODS: The production of iron oxide nanoparticle conjugated with DTPA and Trastuzumab was made using phosphorylethanolamine (PEA) surface modification. Both radiolabeling process was made by the direct radiolabeling of the nanoparticles. The in vivo assay was done in female Balb/c nude mice xenografted with breast cancer. Also a planar imaging using the radiolabeled nanoparticle was performed. RESULTS: No thrombus and immune response leading to unwanted interaction and incorporation of nanoparticles by endothelium and organs, except filtration by the kidneys, was observed. In fact, more than 80% of Tc-DTPA-TZMB@Fe O nanoparticles seems to be cleared by the renal pathway but the implanted tumor whose seems to increase the expression of HER2 receptors enhancing the uptake by all other organs. CONCLUSION: However, even in this unfavorable situation the tumor bioconcentrated much larger amounts of the nano-agent than normal tissues giving clear enough contrast for breast cancer imaging for diagnostics purpose by both SPECT and PET technique. Graphical Abstract á…Ÿ.
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1801
[Cu] Class update date: 180202
[Lr] Last revision date:180202
[St] Status:In-Process
[do] DOI:10.1007/s11095-017-2320-2

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[PMID]: 29124423
[Au] Autor:Lu Q; Li J; Chen F; Liao M; Lin L; Tang Y; Liang D; Xia H; Lai Y; Wang X; Chen C; Ren W
[Ad] Address:College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
[Ti] Title:Effects of mutual intercropping on the cadmium accumulation in accumulator plants Stellaria media, Malachium aquaticum, and Galium aparine.
[So] Source:Environ Monit Assess;189(12):622, 2017 Nov 09.
[Is] ISSN:1573-2959
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Intercropping affects heavy-metal uptake in plants. In this study, three cadmium (Cd)-accumulator species (Stellaria media, Malachium aquaticum, and Galium aparine) were intercropped together in Cd-contaminated soil to study the effects of intercropping on co-remediation. Mutual intercropping significantly increased the biomasses of S. media, M. aquaticum, and G. aparine compared with their respective monocultures. The photosynthetic pigment contents of three species were not significantly affected by mutual intercropping. Mutual intercropping did not increase the Cd contents in roots and shoots of G. aparine, but it increased the Cd contents in roots of S. media and M. aquaticum. It also decreased the Cd contents in shoots of S. media and M. aquaticum. Only G. aparine intercropped with M. aquaticum and three-species intercropping increased Cd-accumulation levels in whole plants of each species. Only S. media intercropped with M. aquaticum and three-species intercropping increased the Cd accumulation at the whole plant level in every plant in a single pot, with S. media intercropped with M. aquaticum showing the greatest increase. Therefore, S. media intercropped with M. aquaticum and three-species intercropping may improve the efficiency of phytoremediation of Cd-contaminated soil, with S. media intercropped with M. aquaticum representing the best combination.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1711
[Cu] Class update date: 171114
[Lr] Last revision date:171114
[St] Status:In-Process
[do] DOI:10.1007/s10661-017-6322-7

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[PMID]: 28874171
[Au] Autor:Carello R; Ricottini L; Miranda V; Panei P; Rocchi L; Arcieri R; Galli E
[Ad] Address:Department of Pediatric Allergy, San Pietro Hospital Fatebenefratelli, Via Cassia, 600, Rome, Italy. carellorossella@gmail.com.
[Ti] Title:Long-term treatment with low-dose medicine in chronic childhood eczema: a double-blind two-stage randomized control trial.
[So] Source:Ital J Pediatr;43(1):78, 2017 Sep 06.
[Is] ISSN:1824-7288
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: The efficacy of low-dose medicine (LDM) in childhood mild/moderate eczema is not known. We conducted a double-blind, two-stage, randomized, placebo-controlled clinical trial, lasting 23 months, to address this issue. METHOD: Eighty children with chronic mild/moderate eczema were randomly allocated to Group A (placebo) or Group B (treatment group; Galium-Heel®, a low-dose multicomponent medicine based upon natural substances; Guna-Interleukin 12 and Guna-Interferon-γ administered twice a day for six non-consecutive months for each stage). LDM is characterized by the use of biological molecules, such as cytokines, neuropeptides, growth factors, hormones at very low concentrations, which correspond to physiological levels within the human body. The dosage of the cytokines used in this trial (IFN-γ and IL-12) is 10 fg/ml. The SCORAD index was evaluated by the same operator: subjects with a SCORAD index below 20 were considered to have mild eczema (61/80; mean: 10.79), whereas a SCORAD index between 20-50 indicated moderate eczema (19/80; mean: 26.84). The data of 66/80 children were analyzed in stage 1 and those of 62/66 children in stage 2. The primary outcome measure was reduction of eczema severity assessed by the SCORAD index. Secondary outcomes were disease-free interval, and treatment safety and tolerability. RESULTS: The decrease in disease severity was greater in Group B than in Group A already in stage 1 (a decrease 63.9% versus 53.2%), but the difference was not significant (p = 0.16). Moreover, subjective symptoms (itching and sleep disturbances) initially decreased and then worsened in Group A, whereas itching decreased linearly and sleep disturbances decreased significantly (p=0.049) in Group B. CONCLUSIONS: Preliminary evidence suggests potential benefit, but further work is needed to validate this approach. TRIAL REGISTRATION: The trial was registered with EudraCT number 2010-018640-13 through the database of the National Clinical Trials Monitoring Centre Database (Osservatorio delle Sperimentazioni Cliniche, OsSC) of the Italian Medicines Agency.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1709
[Cu] Class update date: 170910
[Lr] Last revision date:170910
[St] Status:In-Process
[do] DOI:10.1186/s13052-017-0393-5

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[PMID]: 28850863
[Au] Autor:Stajic A; Andelkovic M; Dikic N; Rasic J; Vukasinovic-Vesic M; Ivanovic D; Jancic-Stojanovic B
[Ad] Address:Department of Drug Analysis, University of Belgrade - Faculty of Pharmacy, Vojvode Stepe 450, Belgrade, Serbia.
[Ti] Title:Determination of higenamine in dietary supplements by UHPLC/MS/MS method.
[So] Source:J Pharm Biomed Anal;146:48-52, 2017 Nov 30.
[Is] ISSN:1873-264X
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:From 1st January 2017 higenamine was added on the WADA (World Anti-doping Agency) Prohibited list under S3 group beta-2 agonists as at all times banned substance for the athletes. The main origine of higenamine (or norcoclaurine) are different plants including Nandina domestica, Aconitum carmichaelii, Asarum heterotropioides, Galium divaricatum, Annona squamosa, Nelumbo nucifera etc. Higenamine main use is related to weight loss and it could be found (un)labeled in different dietary supplements. The objective of this study was development of sensitive and reliable UHPLC/MS/MS method for determination of higenamine in various dietary supplement samples. In order to obtain high method sensitivity, hydrophilic interaction liquid chromatography (HILIC) mode was applied. Separation was carried out on UHPLC Acquity BEH HILIC analytical column (2.1mm×100mm, 1.7µm particle size). Mobile phase consisted of 0.1% formic acid in water and acetonitrile, respectively, was mixed in ratio of 30:70, v/v. Flow rate was set at 0.2mLmin . Quercetin was used as an internal standard. ESI (+) source ionization mode using multi reaction monitoring (MRM) mode was utilized and three ion transitions of higenamine were followed 272.08→107.01, 272.08→161.07 and 272.08→77.08. Developed method was fully validated and applied for identification and quantification of higenamine in different dietary supplements. According to the results, the most of investigated supplements were free of higenamine, and on the other hand, presence of higenamine was confirmed in some samples while it was not declared on the label.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1708
[Cu] Class update date: 171007
[Lr] Last revision date:171007
[St] Status:In-Process

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[PMID]: 28817656
[Au] Autor:Heußer T; Mann P; Rank CM; Schäfer M; Dimitrakopoulou-Strauss A; Schlemmer HP; Hadaschik BA; Kopka K; Bachert P; Kachelrieß M; Freitag MT
[Ad] Address:Medical Physics in Radiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
[Ti] Title:Investigation of the halo-artifact in 68Ga-PSMA-11-PET/MRI.
[So] Source:PLoS One;12(8):e0183329, 2017.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:OBJECTIVES: Combined positron emission tomography (PET) and magnetic resonance imaging (MRI) targeting the prostate-specific membrane antigen (PSMA) with a 68Ga-labelled PSMA-analog (68Ga-PSMA-11) is discussed as a promising diagnostic method for patients with suspicion or history of prostate cancer. One potential drawback of this method are severe photopenic (halo-) artifacts surrounding the bladder and the kidneys in the scatter-corrected PET images, which have been reported to occur frequently in clinical practice. The goal of this work was to investigate the occurrence and impact of these artifacts and, secondly, to evaluate variants of the standard scatter correction method with regard to halo-artifact suppression. METHODS: Experiments using a dedicated pelvis phantom were conducted to investigate whether the halo-artifact is modality-, tracer-, and/or concentration-dependent. Furthermore, 31 patients with history of prostate cancer were selected from an ongoing 68Ga-PSMA-11-PET/MRI study. For each patient, PET raw data were reconstructed employing six different variants of PET scatter correction: absolute scatter scaling, relative scatter scaling, and relative scatter scaling combined with prompt gamma correction, each of which was combined with a maximum scatter fraction (MaxSF) of MaxSF = 75% or MaxSF = 40%. Evaluation of the reconstructed images with regard to halo-artifact suppression was performed both quantitatively using statistical analysis and qualitatively by two independent readers. RESULTS: The phantom experiments did not reveal any modality-dependency (PET/MRI vs. PET/CT) or tracer-dependency (68Ga vs. 18F-FDG). Patient- and phantom-based data indicated that halo-artifacts derive from high organ-to-background activity ratios (OBR) between bladder/kidneys and surrounding soft tissue, with a positive correlation between OBR and halo size. Comparing different variants of scatter correction, reducing the maximum scatter fraction from the default value MaxSF = 75% to MaxSF = 40% was found to efficiently suppress halo-artifacts in both phantom and patient data. In 1 of 31 patients, reducing the maximum scatter fraction provided new PET-based information changing the patient's diagnosis. CONCLUSION: Halo-artifacts are particularly observed for 68Ga-PSMA-11-PET/MRI due to 1) the biodistribution of the PSMA-11-tracer resulting in large OBRs for bladder and kidneys and 2) inaccurate scatter correction methods currently used in clinical routine, which tend to overestimate the scatter contribution. If not compensated for, 68Ga-PSMA-11 uptake pathologies may be masked by halo-artifacts leading to false-negative diagnoses. Reducing the maximum scatter fraction was found to efficiently suppress halo-artifacts.
[Mh] MeSH terms primary: Artifacts
Galium/chemistry
Magnetic Resonance Imaging
Positron-Emission Tomography
[Mh] MeSH terms secundary: Humans
Male
Middle Aged
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1710
[Cu] Class update date: 171016
[Lr] Last revision date:171016
[Js] Journal subset:IM
[Da] Date of entry for processing:170818
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183329

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[PMID]: 28606843
[Au] Autor:Cheng C; Zhang H; Li Y; Zhou Y; Lu W; Yao L
[Ad] Address:College of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150000, China.
[Ti] Title:The effect of Diosmin on the blood proteome in a rat model of venous thrombosis.
[So] Source:Int J Biol Macromol;104(Pt A):778-787, 2017 Nov.
[Is] ISSN:1879-0003
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Diosmin is the aglycone moiety of diosmetin (3',5,7 trihydroxy-4'methoxy radicals flavonoids), a naturally occurring flavone glycoside, whose antithrombotic effect was studied in rats. This study was designed to find the protein changes of venous thrombosis in Wistar rats comparing conditions with and without Diosmin treatment by two-dimensional gel electrophoresis (2-DE), and investigate the effect of a crucial protein known as CEP350 on human vascular endothelial cell growth. Through prior chromatographic purification with macroporous absorption resin (AB-8) and polyamide, Diosmin was isolated from Galium verum L. by solvent extraction, then purified to 98% purity using HPLC. Wistar rats were divided into control group, model group, and prevention group. And their venous thrombosis tissue segments were dissected and prepared for histopathological examination and detection of plasma protein C (PC). Next, proteomic analysis was performed with the samples. Low-abundance proteins of the three groups were separated by two-dimensional gel electrophoresis (2-DE). 2-DE analysis revealed that 191 protein spots were differentially expressed among those three groups. For protein identification, we selected six spots to use matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) detection, and then do the homology search in NCBI database. Considering characteristics of these proteins, we proposed CEP350 is related to spindle assembly. Furthermore, we used Lipofectamine 2000 to transfect HUVECs with CEP350 siRNA and evaluated the extent of silencing using real time-polymerase chain reaction (RT-PCR). Cells were stained for immunofluorescence with tubulin-tracker red, and structural changes were analyzed using laser scanning confocal microscope. We concluded that CEP350 depletion decreased microtubule stability. Dosmin could modulate the assemble of spindle from unevenly distributing and protect body from varicose veins by regulating CEP350.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1706
[Cu] Class update date: 170906
[Lr] Last revision date:170906
[St] Status:In-Process

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[PMID]: 28534675
[Au] Autor:Parravicini J; Acciarri M; Lomuscio A; Murabito M; Le Donne A; Gasparotto A; Binetti S
[Ad] Address:1 Dipartimento di Scienza dei Materiali and MIBSOLAR, Università di Milano-Bicocca, Milano, Italy.
[Ti] Title:Gallium In-Depth Profile in Bromine- Etched Copper-Indium-Galium-(Di)selenide (CIGS) Thin Films Inspected Using Raman Spectroscopy.
[So] Source:Appl Spectrosc;71(6):1334-1339, 2017 Jun.
[Is] ISSN:1943-3530
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:In the thin film solar cells domain, copper indium galium (di)selenide (CIGS) is a material with well-established photovoltaic purpose. Here the presence of a suitable [Ga]/([Ga]+[In]) (GGI) in-depth profile has proved to play a key role in the performance of cells. The implementation of a routine method based on reliable but easily available experimental techniques is mandatory to obtain information on the GGI profile of any CIGS layer, in order to achieve high efficiency chalcogenide layers. In this vein, we here propose and systematically test a simple method for the GGI profile determination based on repeated bromine etching of CIGS thin films followed by Raman analysis of the A peak position. The reliability of the proposed approach is verified using a methodical comparison with energy-dispersive X-ray spectroscopy (EDS) analysis and secondary ion mass spectroscopy (SIMS) profiles, showing a good agreement with the GGI in-depth profiles determined using Raman analysis on bromine etched samples.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1705
[Cu] Class update date: 170523
[Lr] Last revision date:170523
[St] Status:In-Data-Review
[do] DOI:10.1177/0003702816681568

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[PMID]: 28109206
[Au] Autor:Aydogan EL; Busse HJ; Moser G; Müller C; Kämpfer P; Glaeser SP
[Ad] Address:1​Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
[Ti] Title:Proposal of Mucilaginibacter galii sp. nov. isolated from leaves of Galium album.
[So] Source:Int J Syst Evol Microbiol;67(5):1318-1326, 2017 May.
[Is] ISSN:1466-5034
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:A pale-pink-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterial strain, PP-F2F-G47T, was isolated from the phyllosphere of the herbaceous plant Galium album. Phylogenetic analysis based on the nearly full-length 16S rRNA gene sequence revealed highest sequence similarity to the type strains of Mucilaginibacter daejeonensis (96.2 %), Mucilaginibacter dorajii (95.7 %) and Mucilaginibacter phyllosphaerae (95.5 %). 16S rRNA gene sequence similarities to all other type strains were below 95.5 %. The predominant cellular fatty acids of the strain were C16 : 1ω7c/iso-C15 : 0 2-OH (measured as summed feature 3) and iso-C15 : 0. The major compound in the polyamine pattern was sym-homospermidine and major quinone was menaquinone MK-7. The polar lipid profile was composed of phosphatidylethanolamine and several unidentified aminolipipids, phospholipids, aminophospholipids and lipids without a functional group. A sphingophospholipid could not be detected but a ninhydrin-positive alkaline-stable lipid was visible. The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid. Based on phylogenetic, chemotaxonomic and phenotypic analyses a novel species is proposed, Mucilaginibacter galii sp. nov., with PP-F2F-G47T (=CCM 8711T=CIP 111182T=LMG 29767T) as the type strain.
[Mh] MeSH terms primary: Bacteroidetes/classification
Galium/microbiology
Phylogeny
[Mh] MeSH terms secundary: Bacterial Typing Techniques
Bacteroidetes/genetics
Bacteroidetes/isolation & purification
Base Composition
DNA, Bacterial/genetics
Diaminopimelic Acid/chemistry
Fatty Acids/chemistry
Germany
Phospholipids/chemistry
Plant Leaves/microbiology
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA
Spermidine/analogs & derivatives
Spermidine/chemistry
Vitamin K 2/analogs & derivatives
Vitamin K 2/chemistry
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 0IJ25X1H4R (sym-homospermidine); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 8427BML8NY (vitamin MK 7); U87FK77H25 (Spermidine)
[Em] Entry month:1708
[Cu] Class update date: 170825
[Lr] Last revision date:170825
[Js] Journal subset:IM
[Da] Date of entry for processing:170122
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.001808

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[PMID]: 28879756
[Au] Autor:Liu HL; Cheng ME; Peng DY
[Ad] Address:Chinese Materia Medica Resource Center, Anhui University of Chinese Medicine, Hefei 230012, China.
[Ti] Title:[New records of distribution of medical plants in Anhui province].
[So] Source:Zhongguo Zhong Yao Za Zhi;41(7):1358-1360, 2016 Apr.
[Is] ISSN:1001-5302
[Cp] Country of publication:China
[La] Language:chi
[Ab] Abstract:During the fourth national survey of Chinese material medica resources inventory, 9 species of medical plants in Huangshan area of Anhui Province were newly recorded, including Microlepia calvescens, Dryopteris hangchowensis, Fatoua pilosa, Girardinia chingiana, Lecanthus peduncularis, Galium kamtschaticum, Carpesium minus,Cirsium racemiforme, Globba racemosa, which belong to seven families and nine genera. Among these, 3 genera (Girardinia, Lecanthus, Globba) are new geographical distribution in Anhui Province. All of voucher specimens are preserved in ACM. These discoveries enrich the content of flora in Anhui and provide fundamental materials for studying the plants of Anhui.
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Entry month:1709
[Cu] Class update date: 170907
[Lr] Last revision date:170907
[St] Status:In-Process
[do] DOI:10.4268/cjcmm20160732


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