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[PMID]: 29408624
[Au] Autor:Wang X; Li W; Xu X; Wang W; He K; Fan H
[Ad] Address:Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China; College of Veterinary Medicine, Nanjing
[Ti] Title:Phylogenetic analysis of two goat-origin PCV2 isolates in China.
[So] Source:Gene;651:57-61, 2018 Apr 20.
[Is] ISSN:1879-0038
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Complete genome characterization of non-porcine origin Porcine circovirus type 2 (PCV2) was first described in 2014 in China. In the present study, we first identified PCV2 nucleotides in goat samples and the prevalence of PCV2 in goat was 6.15%. However, only two new strains, Goat2014-4 and Goat2014-5, could be completely sequenced. The genome of the strain Goat2014-4, which collected from the goat infected with PPRV, contains 1766 nt; strain Goat2014-5, which originated from a healthy goat, is comprised of 1767 nt. The results showed that they shared the highest nucleotide identity with BDH and the lowest similarity with DK1980PMWSfree strain and they belonged only to genotype PCV2d. Meanwhile, they shared higher homology with porcine-origin PCV2 strains than others. Moreover, a detailed analysis of the capsid amino acid sequences revealed that there were distinct differences for goat2014-4 (708 bp) and goat2014-5 (705 bp); strain Goat2014-4 showed an elongation of two amino acids, and strains Goat2014-5 showed an elongation of one amino acid compared with other reference strains. This is the first report of the genetic analysis of goat-origin PCV2 isolates. It also provides an additional supported evidence for cross-species transmission of PCV2.
[Mh] MeSH terms primary: Circoviridae Infections/veterinary
Circovirus/classification
Circovirus/isolation & purification
Goat Diseases/virology
[Mh] MeSH terms secundary: Animals
Capsid Proteins/genetics
China
Circoviridae Infections/virology
Gene Frequency
Genome, Viral
Goats
Phylogeny
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Capsid Proteins)
[Em] Entry month:1803
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[Js] Journal subset:IM
[Da] Date of entry for processing:180207
[St] Status:MEDLINE

  2 / 9537 MEDLINE  
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[PMID]: 29246990
[Ti] Title:Mortality due to pulpy kidney in beef calves and goats.
[So] Source:Vet Rec;181(24):646-650, 2017 12 16.
[Is] ISSN:2042-7670
[Cp] Country of publication:England
[La] Language:eng
[Mh] MeSH terms primary: Animal Diseases/epidemiology
Disease Outbreaks/veterinary
Sentinel Surveillance/veterinary
[Mh] MeSH terms secundary: Animals
Bird Diseases/epidemiology
Birds
Cattle
Cattle Diseases/epidemiology
Cattle Diseases/mortality
Female
Goat Diseases/epidemiology
Goat Diseases/mortality
Goats
Kidney Diseases/mortality
Kidney Diseases/veterinary
Poultry
Pregnancy
Scotland/epidemiology
Sheep
Sheep Diseases/epidemiology
Swine
Swine Diseases/epidemiology
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[Js] Journal subset:IM
[Da] Date of entry for processing:171217
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j5822

  3 / 9537 MEDLINE  
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[PMID]: 29246991
[Ti] Title: type D enterotoxaemia in cattle, goats and sheep.
[So] Source:Vet Rec;181(24):648-649, 2017 12 16.
[Is] ISSN:2042-7670
[Cp] Country of publication:England
[La] Language:eng
[Mh] MeSH terms primary: Cattle Diseases/diagnosis
Clostridium perfringens/isolation & purification
Enterotoxemia/diagnosis
Goat Diseases/diagnosis
Sheep Diseases/diagnosis
[Mh] MeSH terms secundary: Animals
Bacterial Toxins/isolation & purification
Cattle
Cattle Diseases/microbiology
Clostridium perfringens/classification
Enterotoxemia/microbiology
Gastrointestinal Contents
Goat Diseases/microbiology
Goats
Sentinel Surveillance/veterinary
Sheep
Sheep Diseases/microbiology
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Bacterial Toxins)
[Em] Entry month:1803
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[Js] Journal subset:IM
[Da] Date of entry for processing:171217
[St] Status:MEDLINE
[do] DOI:10.1136/vr.j5823

  4 / 9537 MEDLINE  
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[PMID]: 29256284
[Au] Autor:Fbin R; Kovcs A; Stger V; Frank K; Egerszegi I; Olh J; Bod S
[Ad] Address:1 National Agricultural Research and Innovation Centre , Szent-Gyrgyi Albert u. 4, H-2100 Gdllo , Hungary.
[Ti] Title:X- and Y-chromosome-specific variants of the amelogenin gene allow non-invasive sex diagnosis for the detection of pseudohermaphrodite goats.
[So] Source:Acta Vet Hung;65(4):500-504, 2017 12.
[Is] ISSN:0236-6290
[Cp] Country of publication:Hungary
[La] Language:eng
[Ab] Abstract:The Polled Intersex Syndrome (PIS) is responsible for the absence of horns in homozygous and heterozygous goats causing a female-to-male sex reversal in the homozygous polled genotypic female (XX) goats. A simple and efficient non-invasive method was elaborated to detect the genotypic sex from hair and faecal samples using a pair of primers to amplify the X- and Y-linked alleles of the amelogenin gene. The PCR products were easily distinguishable using agarose gel electrophoresis: we detected an X-specific single band in samples originating from healthy phenotypic females and double (X- and Y-) bands in samples from males. The new PCR method is applicable for diagnosing the sex of PIS-affected animals already as newborn kids, in contrast with the phenotypic findings appearing only after puberty, and thus it may replace the cumbersome chromosome investigations.
[Mh] MeSH terms primary: Amelogenin/metabolism
Disorders of Sex Development/veterinary
Goat Diseases/genetics
X Chromosome/genetics
Y Chromosome/genetics
[Mh] MeSH terms secundary: Amelogenin/genetics
Animals
DNA/chemistry
DNA/genetics
Disorders of Sex Development/diagnosis
Disorders of Sex Development/genetics
Feces/chemistry
Female
Gene Expression Regulation
Genotype
Goat Diseases/diagnosis
Goats
Hair/chemistry
Male
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Amelogenin); 9007-49-2 (DNA)
[Em] Entry month:1803
[Cu] Class update date: 180305
[Lr] Last revision date:180305
[Js] Journal subset:IM
[Da] Date of entry for processing:171220
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.047

  5 / 9537 MEDLINE  
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[PMID]: 29243405
[Au] Autor:Brard E; Schulz C; Sailleau C; Bernelin-Cottet C; Viarouge C; Vitour D; Guillaume B; Caignard G; Gorlier A; Attoui H; Gallois M; Hoffmann B; Zientara S; Beer M
[Ad] Address:Laboratoire de Sant Animale d'Alfort, Universit Paris Est, ANSES, ENVA, INRA, UMR 1161 VIROLOGIE, Maisons-Alfort, France.
[Ti] Title:Bluetongue virus serotype 27: Experimental infection of goats, sheep and cattle with three BTV-27 variants reveal atypical characteristics and likely direct contact transmission BTV-27 between goats.
[So] Source:Transbound Emerg Dis;65(2):e251-e263, 2018 Apr.
[Is] ISSN:1865-1682
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Bluetongue virus (BTV) hitherto consisted of 26 recognized serotypes, of which all except BTV-26 are primarily transmitted by certain species of Culicoides biting midges. Three variants of an additional 27th bluetongue virus serotype (BTV-27v01-v03) were recently detected in asymptomatic goats in Corsica, France, 2014-2015. Molecular characterization revealed genetic differences between the three variants. Therefore, invivo characteristics were investigated by experimental infection of a total of 15 goats, 11 sheep and 4 cattle with any one of the three variants in separated animal trials. In goat trials, BTV-nave animals of the same species were kept in a facility where direct contact was unhindered. Of the 15 inoculated goats, 13 and 14 animals were found positive for BTV-RNA and antibodies (Ab), respectively, until the end of the experiments. Surprisingly, BTV-Ab levels as measured with ELISA and neutralization test (SNT) were remarkably low in all seropositive goats. Virus isolation from whole-blood was possible at the peak of viremia until 49dpi. Moreover, detection of BTV-27v02-RNA and Ab in one contact goat indicated that-similar to BTV-26-at least one of three BTV-27 variants may be transmitted by contact between goats. In the field, BTV-27 RNA can be detected up to 6months in the whole-blood of BTV-27-infected Corsican goats. In contrast, BTV RNA was not detected in the blood of cattle or sheep. In addition, BTV-27 Abs were not detected in cattle and only a transient increase in Ab levels was observed in some sheep. None of the 30 animals showed obvious BT-like clinical signs. In summary, the phenotypes observed for BTV-27v01-v03 phenotypes correspond to a mixture of characteristics known for BTV-25 and 26.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180302
[Lr] Last revision date:180302
[St] Status:In-Process
[do] DOI:10.1111/tbed.12780

  6 / 9537 MEDLINE  
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[PMID]: 29460417
[Au] Autor:Peck ME; Chanachai K; Jenpanich C; Amonsin A; Alexander BH; Bender JB
[Ad] Address:Environmental Health Sciences, University of Minnesota School of Public Health, Minneapolis, MN, USA.
[Ti] Title:Seroprevalence of brucellosis in goats and sheep in Thailand: Results from the Thai National Brucellosis Surveillance System from 2013 to 2015.
[So] Source:Transbound Emerg Dis;, 2018 Feb 19.
[Is] ISSN:1865-1682
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:In Thailand, brucellosis re-emerged in humans in 2003 and is considered a public health risk to goat farmers as the disease is endemic in small ruminants. The Thai Department of Livestock Development (DLD) established a nationwide surveillance system for brucellosis in goats and sheep in 1997. Using data from this surveillance system, we describe the seroprevalence of brucellosis from 2013 to 2015 in small ruminants and the spatial distribution of the disease throughout Thailand. Surveillance data collected included the number of animals and herds tested, the province of the animal and herd and the laboratory results. Seroprevalence was estimated at both the animal and herd levels. During the 3-year period, 443,561 goats and sheep were tested for brucellosis by the DLD throughout Thailand using the Rose Bengal PlateTest (RBPT) and the enzyme-linked immunosorbent assay test for Brucella. Among the 3years, 2013 had the highest proportion of herds that tested positive for brucellosis at 13.80% (95% CI, 12.52, 15.16). Overall, this study found that brucellosis seroprevalence in small ruminants is decreasing throughout Thailand. However, there is variability in the spread of the disease with provinces in the eastern and western regions of Thailand having higher proportions of animals and herds testing positive. Overall provinces in the south had the lowest proportion of animals and herds testing positive for brucellosis. Periodic review of surveillance data documents the impact of the current brucellosis control programme and supports a targeted response in higher prevalence regions when there are limited financial resources for control measures.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180227
[Lr] Last revision date:180227
[St] Status:Publisher
[do] DOI:10.1111/tbed.12826

  7 / 9537 MEDLINE  
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[PMID]: 29476407
[Au] Autor:Chaka H; Aboset G; Garoma A; Gumi B; Thys E
[Ad] Address:National Animal Health Diagnostic and Investigation Centre (NAHDIC), P.O. Box 34, Sebeta, Ethiopia. hasscha@yahoo.com.
[Ti] Title:Cross-sectional survey of brucellosis and associated risk factors in the livestock-wildlife interface area of Nechisar National Park, Ethiopia.
[So] Source:Trop Anim Health Prod;, 2018 Feb 24.
[Is] ISSN:1573-7438
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:A cross-sectional survey was carried out to investigate the seroprevalence of ovine and bovine brucellosis in the livestock-wildlife interface area of Nechisar National Park, Ethiopia. Furthermore, producer's knowledge about brucellosis and its zoonotic potential was assessed using a structured questionnaire. A total of 268 cattle and 246 goat sera were collected from 50 herds and 46 flocks and subjected to Rose Bengal test (RBT) and enzyme-linked immunosorbent assay (ELISA) in parallel to detect anti-Brucella species antibodies. Positive reactions were further confirmed with compliment fixation test (CFT). Flock and herd level seroprevalence rate was 12.8% (95% CI 4.8-25.7) and 32.0% (95% CI 19.5-46.7) in goats and cattle, respectively. An overall animal-level seroprevalence of 4.5% (95% CI 2.25-7.86) and 9.7% (95% CI 6.44-13.89) was recorded for goats and cattle, respectively. Seroprevalence showed an increasing trend with age, where adult cattle > 2years. Goats (> 1year) recorded relatively higher seroprevalence, but the differences were not statistically significant. Similarly, female cattle and goats recorded a relatively higher seroprevalence, 11 and 5.6%, respectively, compared to males but the difference was not significant. However, a significant (P< 0.01) variation of seroprevalence was noted for parity (bovine), higher in animals in second parity, and abortion history, in both species, higher in animals that experienced abortion. Interviews revealed lack of awareness about brucellosis and food safety related to the zoonotic potential from consuming raw animal products (milk and meat). Ninety-eight percent of respondents did not consider handling abortion material is risky, and only a very low proportion (8%, n = 50) was able to mention limited zoonotic diseases (anthrax and Taenia cysticercosis) could be transmissible to people. The study indicated that brucellosis is endemic in domestic animals in the interface area and calls for further broad epidemiological investigation of the disease in livestock, human and wildlife following 'one health' unified research approaches beside enhancing public awareness.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180224
[Lr] Last revision date:180224
[St] Status:Publisher
[do] DOI:10.1007/s11250-018-1528-4

  8 / 9537 MEDLINE  
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[PMID]: 29469370
[Au] Autor:Ning T; Qiang C; Chao-Pin L
[Ad] Address:Department of Medical Parasitology, Wannan Medical College, Wuhu 241002, China.
[Ti] Title:[ found in Huainan area in Anhui Province].
[So] Source:Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi;29(5):647, 2017 Mar 02.
[Is] ISSN:1005-6661
[Cp] Country of publication:China
[La] Language:chi
[Ab] Abstract:OBJECTIVE: To study the morphological characteristics of in order to provide the evidence for future research. METHODS: A group of goats were randomly selected and checked one by one, and the ticks were collected. RESULTS: A hard tick was found on the surface of a goat, and it was identified as . CONCLUSIONS: has been found in Huainan area in Anhui Province, confirming that Huainan area is a new breeding place of . This finding may contribute to the epidemiological research of tick-borne diseases.
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180222
[Lr] Last revision date:180222
[St] Status:In-Process
[do] DOI:10.16250/j.32.1374.2016173

  9 / 9537 MEDLINE  
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[PMID]: 29460862
[Au] Autor:Zhang C; Shi G; Cheng P; Liu L; Gong M
[Ad] Address:Department of Medical Entomology, Vector Biology Key Laboratory of Medicine and Health Shandong Province, Shandong Institute of Parasitic Diseases, Shandong Academy of Medical Sciences, Jining; Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medica
[Ti] Title:Host preferences and feeding patterns of Wiedemann in three sites of Shandong province, China.
[So] Source:J Vector Borne Dis;54(4):328-333, 2017 Oct-Dec.
[Is] ISSN:0972-9062
[Cp] Country of publication:India
[La] Language:eng
[Ab] Abstract:Background & objectives: Anopheles sinensis Wiedemann is a major vector of malaria and is among the dominant species in Shandong province of China. Knowledge of the blood-feeding patterns of mosquitoes is crucial for elimination of malaria vectors. However, little information is available on the blood-feeding behaviour of An. sinensis mosquitoes in Shandong province. This study was carried out to compare the blood-feeding behaviour of An. sinensis in malaria-endemic areas of Shandong province China. Methods: Adult Anopheles mosquitoes were collected from three malaria-endemic areas (Jimo, Yinan and Shanxian), during the peak months of mosquito population (August and September) from 2014 to 2015. Indoor-resting mosquitoes and outdoor-resting blood-fed females were sampled in the morning hours (0600 to 0900 hrs) from 10 randomly selected houses using pyrethrum spray catch method, and sweeping with an insect net. ELISA was used for the identification of blood meal. The blood meal of each mosquito was tested against antisera specific to human, pig, dog, cow, goat, horse (mule) and fowl. Results: At all indoor study locations of Jimo, Yinan and Shanxian, 59.4, 68.1 and 98.8% blood-engorged female An. sinensis collected from cattle sheds fed almost exclusively on bovines, respectively. For outdoor locations, at Jimo site, 27.27 and 49.55% An. sinensis fed on cattle and pigs; at Yinan, 30.42% fed on cattle and 36.88% fed both on cattle and goats, while no pig antibodies were detected. At Shanxian, percent of An. sinensis that fed on cattle, pigs and cattle-goat was 20.72, 27.62 and 21.78%, respectively. Interpretation & conclusion: The analysis of An. sinensis blood meals in all the three studied areas from human houses, cattle sheds, pig sheds and mixed dwellings revealed that An. sinensis prefers cattle hosts, and can feed on other available animal hosts if the cattle hosts are absent, and the mosquitoes readily feed on humans when domestic animals (cattle and pigs) are not nearby for feeding. The analysis of blood meal revealed that An. sinensis follow opportunistic feeding in Shandong province, China.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180220
[Lr] Last revision date:180220
[St] Status:In-Process
[do] DOI:10.4103/0972-9062.225837

  10 / 9537 MEDLINE  
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[PMID]: 29408555
[Au] Autor:Paulsamy M; Ponnusamy C; Palanisami M; Nackeeran G; Paramasivam S; Sugumaran A; Kandasamy R; Natesan S; Palanichamy R
[Ad] Address:Department of BioTechnology, University College of Engineering, BIT Campus, Anna University, Tiruchirappalli, Tamil Nadu, India.
[Ti] Title:Nepafenac loaded silica nanoparticles dispersed in-situ gel systems: Development and characterization.
[So] Source:Int J Biol Macromol;, 2018 Jan 31.
[Is] ISSN:1879-0003
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:This research was aimed to develop and evaluate nepafenac loaded silica nanoparticles dispersed in-situ gel system for the improved treatment of ocular diseases. The blank silica nanoparticles prepared by stober's process showed the particle size of 151 nm to 285 nm with the zeta potential of -19.6 to -31.9 mV. The nepafenac loaded silica nanoparticles were spherical in shape with smooth outer surface. The nepafenac loaded silica nanoparticles dispersed in poloxamer - chitosan in-situ gelling system showed gelling temperature of 32 C with sustained release of nepafenac and higher permeation (58.79 g) across the goat cornea than poloxamer - poloxamer (21.18 g) in-situ gelling system. Hence the developed in-situ gelling system containing nepafenac loaded silica nanoparticle could be a promising tool for the topical delivery of drugs to the eye.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180217
[Lr] Last revision date:180217
[St] Status:Publisher


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