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[PMID]: | 26475369 | [Au] Autor: | González-Torres L; Vázquez-Velasco M; Olivero-David R; Bastida S; Benedí J; González RR; González-Muñoz MJ; Sánchez-Muniz FJ |
[Ti] Title: | Glucomannan and glucomannan plus spirulina added to pork significantly block dietary cholesterol effects on lipoproteinemia, arylesterase activity, and CYP7A1 expression in Zucker fa/fa rats. | [So] Source: | J Physiol Biochem;71(4):773-84, 2015 Dec. | [Is] ISSN: | 1877-8755 | [Cp] Country of publication: | Spain | [La] Language: | eng | [Ab] Abstract: | Zucker fa/fa rats easily develop dyslipidemia and obesity. Restructured pork (RP) is a suitable matrix for including functional ingredients. The effects of glucomannan- RP or glucomannan plus spirulina-enriched RP on plasma lipid/lipoprotein levels, cytochrome P450 7A1 (CYP7A1) expression, and arylesterase activity in growing fa/fa rats fed high-energy, high-fat cholesterol-enriched diets were tested. Groups of six rats each received diet containing 15% control-RP (C), 15% glucomannan-RP diet (G), 15% glucomannan + spirulina-RP diet (GS), and same diets enriched with 2.4% cholesterol and 0.49% cholic acid (cholesterol-enriched control (HC), cholesterol-enriched glucomannan (HG), and cholesterol-enriched glucomannan + spirulina (HGS) diets) over a 7-week period. C diet induced obesity, severe hyperglycemia, moderate hypercholesterolemia, and hypertriglyceridemia. Those facts were not significantly modified by G or GS diets. G diet increased CYP7A1 expression but decreased the total cholesterol/high density lipoproteins (HDL)-cholesterol ratio (p < 0.05) vs. C diet. GS vs. G diet increased (p < 0.05) CYP7A1 expression. HC vs. C diet reduced food intake, body weight gain, and plasma glucose (p < 0.01) but increased cholesterolemia (p < 0.01), lipidemia (plasma cholesterol plus triglycerides) (p < 0.001), cholesterol/triglyceride ratio in very low density lipoproteins (VLDL), and HDL (p < 0.05), cholesterol transported by VLDL and intermediate density lipoproteins (IDL) + low density lipoproteins (LDL), total cholesterol/HDL-cholesterol ratio and CYP7A1 expression (at least p < 0.05). HG and HGS diets vs. HC noticeably reduced lipidemia (p < 0.001), normalized VLDL and IDL + LDL lipid composition, and increased CYP7A1 expression (p < 0.01) but did not modify the cholesterol/HDL-cholesterol ratio. HGS vs. HG decreased triglyceridemia, the triglyceride-glucose (TyG) index and increased arylesterase/HDL-cholesterol activity (p < 0.05). In conclusion, G- and GS-RP act as functional foods and notably blocked the dietary cholesterol effects. In addition, HGS-RP improved the glucomannan hypolipidemic effects, increased arylesterase/HDL-cholesterol activity, and decreased insulin resistance. |
[Mh] MeSH terms primary: |
Carboxylic Ester Hydrolases/metabolism Cholesterol 7-alpha-Hydroxylase/metabolism Food, Fortified Hypolipoproteinemias/diet therapy Mannans/administration & dosage Meat
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[Mh] MeSH terms secundary: |
Animals Blood Glucose Cholesterol/blood Cholesterol 7-alpha-Hydroxylase/genetics Diet, High-Fat/adverse effects Gene Expression Hypercholesterolemia/blood Hypercholesterolemia/diet therapy Hypercholesterolemia/enzymology Hypolipoproteinemias/blood Hypolipoproteinemias/enzymology Liver/enzymology Male Rats, Zucker Spirulina/chemistry Sus scrofa Triglycerides/blood
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[Pt] Publication type: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T | [Nm] Name of substance: | 0 (Blood Glucose); 0 (Mannans); 0 (Triglycerides); 36W3E5TAMG ((1-6)-alpha-glucomannan); 97C5T2UQ7J (Cholesterol); EC 1.14.14.23 (CYP7A1 protein, rat); EC 1.14.14.23 (Cholesterol 7-alpha-Hydroxylase); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.2 (arylesterase) | [Em] Entry month: | 1609 | [Cu] Class update date: |
171111 | [Lr] Last revision date: | 171111 | [Js] Journal subset: | IM | [Da] Date of entry for processing: | 151018 | [St] Status: | MEDLINE |
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