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[PMID]: 25372640
[Au] Autor:Wu JQ; Shi J; Dong JF; Jiang YX; Zhuang SH; Yang J
[Ad] Address:Clinical Laboratory,Jinhua Hospital of Zhejiang University,Jinhua 321000,China....
[Ti] Title:[Construction of three HLA-A*0201-peptide tetramers and their preliminary application in detection of vitiligo-specific cytotoxic T lymphocytes].
[So] Source:Zhejiang Da Xue Xue Bao Yi Xue Ban;43(5):553-8, 2014 Sep 25.
[Is] ISSN:1008-9292
[Cp] Country of publication:China
[La] Language:chi
[Ab] Abstract:OBJECTIVE: To construct vitiligo-specific HLA-A*0201-peptide tetramers and to apply the constructed tetramers in detection of vitiligo-specific cytotoxic T lymphocytes (CTL). METHODS:Proteins HLA-A0201*-BSP and 2M were obtained by effective prokaryotic expression. The purified proteins were refolded with vitiligo antigen peptides MelanA 26-35, gp100 209-217, and tyrosinase 1-9, respectively to form HLA-A*0201-peptide complex. The complex was biotinylated by BirA enzyme and purified by gel-filtration chromatography. The tetramers were generated by mixing the complex with phycoerythrin (PE)-streptavidin at a ratio of 4∶1 and identified by Dot-blot assay. The capacity of tetramer to detect vitiligo-specific CTL was analyzed by flow cytometry. RESULTS:The biotinylation of vitiligo-specific HLA-A*0201-peptide tetramers were successfully performed by Dot-blot. Flow cytometry analysis indicated that the tetramer effectively bound to specific CTL from peripheral blood of patients with vitiligo. CONCLUSION:Three kinds of biotinylated vitiligo-specific HLA-A*0201-peptide tetramers have been constructed successfully. The tetramer can detect antigen specific CTL from patients with vitiligo.
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Entry month:1411
[Js] Journal subset:IM
[St] Status:In-Data-Review

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[PMID]: 25374074
[Au] Autor:Hu D; Yang X; Wang W; Xing Y; Wang W; DU Z; Yiu Q; Zhang L; Chen L; Wu J; Zhang R
[Ad] Address:Department of Immunology and Laboratory Medicine, Medical School, Institute of Infection and Immunology, Anhui University of Science and Technology, Huainan 232001, China....
[Ti] Title:[Immunoprotective effect of a plasmid DNA vaccine pCMV-LC3-Ag85B against Mycobaterium tuberculosis].
[So] Source:Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi;30(11):1133-6, 2014 Nov.
[Is] ISSN:1007-8738
[Cp] Country of publication:China
[La] Language:chi
[Ab] Abstract:Objective To construct an autophagy-targeted vaccine harboring the genes encoding Ag85B and microtubule-associated protein light chain-3 (LC3) and to explore its immunoprotection against Mycobacterium tuberculosis (MTB). Methods The pCMV-LC3-Ag85B plasmid was constructed and used to transfect RAW264.7 cells. The level of LC3-Ag85B was detected using Western blotting. Then, BALB/c mice were immunized with pCMV, pCMV-Ag85B and pCMV-LC3-Ag85B plasmid, respectively. In vitro, two weeks after the last immunization, the secretion of IL-2, IFN-γ, IL-4 and IL-10 from Ag85B-stimulated lymphocytes was measured by ELISA. Three months after the last immunization, all mice were challenged with MTB H37Rv via the tail vein and the bacterial loads in their spleens and lungs were determined by colony formation assay. Results The LC3-Ag85B fusion protein was expressed in RAW264.7 cells that had been transfected with pCMV-LC3-Ag85B and the expression level was in a dose-dependent manner. Compared with the pCMV-Ag85B treatment group, pCMV-LC3-Ag85B-immunized mice showed a significant increase of IL-2 and IFN-γ levels and the lower loads of MTB in the spleens and lungs. Conclusion pCMV-LC3-Ag85B can enhance a specific Th1-predominant immunity and a superior immunoprotection against MTB, which may provide a new practical strategy for the development of improved vaccines against MTB.
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Entry month:1411
[Js] Journal subset:IM
[St] Status:In-Data-Review

  3 / 444588 MEDLINE  
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[PMID]: 25372468
[Au] Autor:Ni XJ; Zhang XL; Ou-Yang QW; Qian GW; Wang L; Chen S; Jiang YZ; Zuo WJ; Wu J; Hu X; Shao ZM
[Ad] Address:Department of Breast Surgery, Key Laboratory of Breast Cancer in Shanghai, Fudan University Shanghai Cancer Center, Fudan University, Shanghai, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China....
[Ti] Title:An Elevated Peripheral Blood Lymphocyte-to-Monocyte Ratio Predicts Favorable Response and Prognosis in Locally Advanced Breast Cancer following Neoadjuvant Chemotherapy.
[So] Source:PLoS One;9(11):e111886, 2014.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PURPOSE: Neoadjuvant chemotherapy (NCT) is a standard treatment option for locally advanced breast cancer. However, the lack of an efficient method to predict treatment response and patient prognosis hampers the clinical evaluation of patient eligibility for NCT. An elevated lymphocyte-to-monocyte ratio (LMR) has been reported to be associated with a favorable prognosis for certain hematologic malignancies and for nasopharyngeal carcinoma; however, this association has not been investigated in breast cancer. The purpose of this study was to evaluate whether pre-NCT LMR analysis could predict the prognosis of patients with locally advanced breast cancer. METHODS: A retrospective cohort of 542 locally advanced breast cancer patients (T3/T4 and/or N2/N3 disease) receiving NCT followed by radical surgery was recruited between May 2002 and August 2011 at the Fudan University Shanghai Cancer Center. Counts for pre-NCT peripheral absolute lymphocytes and monocytes were obtained and used to calculate the LMR. RESULTS: Univariate and multivariate analysis revealed that higher LMR levels (≥4.25) were significantly associated with favorable DFS (P = 0.009 and P = 0.011, respectively). Additionally, univariate analysis revealed that a higher lymphocyte count (≥1.5109/L) showed borderline significance for improved DFS (P = 0.054), while a lower monocyte count (<0.4109/L) was associated with a significantly better DFS (P = 0.010). CONCLUSIONS: An elevated pre-NCT peripheral LMR level was a significantly favorable factor for locally advanced breast cancer patient prognosis. This easily obtained variable may serve as a valuable marker to predict the outcomes of locally advanced breast cancer.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1411
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1371/journal.pone.0111886

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[PMID]: 25374650
[Au] Autor:Kiang JG; Zhai M; Liao PJ; Elliott TB; Gorbunov NV
[Ad] Address:Radiation Combined Injury Program, Armed Forces Radiobiology Research Institute, Bethesda, MD 20889, USA ; Department of Radiation Biology, Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA ; Department of Medicine, Uniformed Services University of the Health Sciences, Be...
[Ti] Title:Ghrelin therapy improves survival after whole-body ionizing irradiation or combined with burn or wound: amelioration of leukocytopenia, thrombocytopenia, splenomegaly, and bone marrow injury.
[So] Source:Oxid Med Cell Longev;2014:215858, 2014.
[Is] ISSN:1942-0994
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Exposure to ionizing radiation alone (RI) or combined with traumatic tissue injury (CI) is a crucial life-threatening factor in nuclear and radiological events. In our laboratory, mice exposed to (60)Co-γ-photon radiation (9.5 Gy, 0.4 Gy/min, bilateral) followed by 15% total-body-surface-area skin wounds (R-W CI) or burns (R-B CI) experienced an increment of ≥18% higher mortality over a 30-day observation period compared to RI alone. CI was accompanied by severe leukocytopenia, thrombocytopenia, erythropenia, and anemia. At the 30th day after injury, numbers of WBC and platelets still remained very low in surviving RI and CI mice. In contrast, their RBC, hemoglobin, and hematocrit were recovered towards preirradiation levels. Only RI induced splenomegaly. RI and CI resulted in bone-marrow cell depletion. In R-W CI mice, ghrelin (a hunger-stimulating peptide) therapy increased survival, mitigated body-weight loss, accelerated wound healing, and increased hematocrit. In R-B CI mice, ghrelin therapy increased survival and numbers of neutrophils, lymphocytes, and platelets and ameliorated bone-marrow cell depletion. In RI mice, this treatment increased survival, hemoglobin, and hematocrit and inhibited splenomegaly. Our novel results are the first to suggest that ghrelin therapy effectively improved survival by mitigating CI-induced leukocytopenia, thrombocytopenia, and bone-marrow injury or the RI-induced decreased hemoglobin and hematocrit.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1411
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1155/2014/215858

  5 / 444588 MEDLINE  
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[PMID]: 25338957
[Au] Autor:Wang K; Wu H; Chi M; Zhang J; Wang G; Li H
[Ad] Address:Department of Anesthesiology, The Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China....
[Ti] Title:Electroacupuncture inhibits apoptosis of splenic lymphocytes in traumatized rats through modulation of the TNF-α/NF-κB signaling pathway.
[So] Source:Mol Med Rep;11(1):237-41, 2015 Jan.
[Is] ISSN:1791-3004
[Cp] Country of publication:Greece
[La] Language:eng
[Ab] Abstract:Surgical trauma leads to a severe deterioration of the immune system. Electroacupuncture(EA) may improve the immunodeficiency that occurs following surgery; however, the underlying signaling mechanisms require further study. In the present study, 40 rats were equally randomized into four groups: Control; Control+EA; Trauma; Trauma+EA. EA was applied at the 'Zusanli'(ST36) and 'Lanwei'(Extra37) acupoints, immediately following surgery. The splenicTcells were isolated from the rats 24h after surgery. The apoptotic rate of the lymphocytes was measured by flow cytometric analysis, and western blotting was used to determine the protein expression levels of caspase3, caspase8, tumor necrosis factor(TNF)α and TNF receptor1 (TNFR1). The DNA binding activity of nuclear factor(NF)κB was determined using TransAM ELISAbased kits. The results of the present study showed that surgical trauma induced apoptosis of splenic lymphocytes, and significantly increased the protein expression levels of caspase3 and caspase8. This was accompanied by increased expression levels of TNFα and TNFR1, and a marked reduction in the activity of NFκB in splenicTcells. Administration of EA significantly decreased the expression levels of caspase3, caspase8, TNFα and TNFR1, elevated the activity of NFκB, and suppressed the apoptotic rate of the lymphocytes. The data suggests that EA may inhibit the apoptosis of splenic lymphocytes induced by surgical trauma, and ameliorate the postoperative immunosuppression. This may be mediated by the downregulation of TNFα expression levels and upregulation of the activity of NFκB.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1411
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.3892/mmr.2014.2740

  6 / 444588 MEDLINE  
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[PMID]: 24874580
[Au] Autor:Horvath G; Reglodi D; Brubel R; Halasz M; Barakonyi A; Tamas A; Fabian E; Opper B; Toth G; Cohen M; Szereday L
[Ad] Address:Department of Anatomy, MTA-PTE "Lendulet" PACAP Research Team, University of Pecs, Szigeti u 12, 7624, Pecs, Hungary.
[Ti] Title:Investigation of the Possible Functions of PACAP in Human Trophoblast Cells.
[So] Source:J Mol Neurosci;54(3):320-30, 2014 Nov.
[Is] ISSN:1559-1166
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Pituitary adenylate cyclase activating polypeptide (PACAP) is an endogenous neuropeptide having a widespread distribution both in the nervous system and peripheral organs including the female reproductive system. Both the peptide and its receptors have been shown in the placenta but its role in placental growth, especially its human aspects, remains unknown. The aim of the present study was to investigate the effects of PACAP on invasion, proliferation, cell survival, and angiogenesis of trophoblast cells. Furthermore, cytokine production was investigated in human decidual and peripheral blood mononuclear cells. For in vitro studies, human invasive proliferative extravillous cytotrophoblast (HIPEC) cells and HTR-8/SVneo human trophoblast cells were used. Both cell types were used for testing the effects of PACAP on invasion and cell survival in order to investigate whether the effects of PACAP in trophoblasts depend on the examined cell type. Invasion was studied by standardized invasion assay. PACAP increased proliferation in HIPEC cells, but not in HTR-8 cells. Cell viability was examined using MTT test, WST-1 assay, and annexin V/propidium iodide flow cytometry assay. Survival of HTR-8/SVneo cells was studied under oxidative stress conditions induced by hydrogen peroxide. PACAP as pretreatment, but not as co-treatment, significantly increased the number of surviving HTR-8 cells. Viability of HIPEC cells was investigated using methotrexate (MTX) toxicity, but PACAP1-38 could not counteract its toxic effect. Angiogenic molecules were determined both in the supernatant and the cell lysate by angiogenesis array. In the supernatant, we found that PACAP decreased the secretion of various angiogenic markers, such as angiopoietin, angiogenin, activin, endoglin, ADAMTS-1, and VEGF. For the cytokine assay, human decidual and peripheral blood lymphocytes were separated and treated with PACAP1-38. Th1 and Th2 cytokines were analyzed with CBA assay and the results showed that there were no significant differences in control and PACAP-treated cells. In summary, PACAP seems to play various roles in human trophoblast cells, depending on the cell type and microenvironmental influences.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1411
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1007/s12031-014-0337-0

  7 / 444588 MEDLINE  
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[PMID]: 25374602
[Au] Autor:Fallahi P; Ferrari SM; Politti U; Giuggioli D; Ferri C; Antonelli A
[Ad] Address:Department of Clinical and Experimental Medicine, University of Pisa, Via Savi 10, 56126 Pisa, Italy....
[Ti] Title:Autoimmune and neoplastic thyroid diseases associated with hepatitis C chronic infection.
[So] Source:Int J Endocrinol;2014:935131, 2014.
[Is] ISSN:1687-8337
[Cp] Country of publication:Egypt
[La] Language:eng
[Ab] Abstract:Frequently, patients with hepatitis C virus (HCV) chronic infection have high levels of serum anti-thyroperoxidase and/or anti-thyroglobulin autoantibodies, ultrasonographic signs of chronic autoimmune thyroiditis, and subclinical hypothyroidism, in female gender versus healthy controls, or hepatitis B virus infected patients. In patients with "HCV-associated mixed cryoglobulinemia" (MC + HCV), a higher prevalence of thyroid autoimmune disorders was shown not only compared to controls, but also versus HCV patients without cryoglobulinemia. Patients with MC + HCV or HCV chronic infection show a higher prevalence of papillary thyroid cancer than controls, in particular in patients with autoimmune thyroiditis. Patients with HCV chronic infection, or with MC + HCV, in presence of autoimmune thyroiditis, show higher serum levels of T-helper (Th)1 (C-X-C motif) ligand 10 (CXCL10) chemokine, but normal levels of Th2 (C-C motif) ligand 2 chemokine, than patients without thyroiditis. HCV thyroid infection could act by upregulating CXCL10 gene expression and secretion in thyrocytes recruiting Th1 lymphocytes that secrete interferon-γ and tumor necrosis factor-α. These cytokines might induce a further CXCL10 secretion by thyrocytes, thus perpetuating the immune cascade, which may lead to the appearance of autoimmune thyroid disorders in genetically predisposed subjects. A careful monitoring of thyroid function, particularly where nodules occur, is recommended in HCV patients.
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Em] Entry month:1411
[Da] Date of entry for processing:141106
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1155/2014/935131

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[PMID]: 25374567
[Au] Autor:Piotti G; Palmisano A; Maggiore U; Buzio C
[Ad] Address:Kidney and Pancreas Transplantation Unit, Department of Clinical Medicine, Nephrology and Health Sciences, University Hospital of Parma , Parma , Italy....
[Ti] Title:Vascular endothelium as a target of immune response in renal transplant rejection.
[So] Source:Front Immunol;5:505, 2014.
[Is] ISSN:1664-3224
[Cp] Country of publication:Switzerland
[La] Language:eng
[Ab] Abstract:This review of clinical and experimental studies aims at analyzing the interplay between graft endothelium and host immune system in renal transplantation, and how it affects the survival of the graft. Graft endothelium is indeed the first barrier between self and non-self that is encountered by host lymphocytes upon reperfusion of vascularized solid transplants. Endothelial cells (EC) express all the major sets of antigens (Ag) that elicit host immune response, and therefore represent a preferential target in organ rejection. Some of the Ag expressed by EC are target of the antibody-mediated response, such as the AB0 blood group system, the human leukocyte antigens (HLA), and MHC class I related chain A antigens (MICA) systems, and the endothelial cell-restricted Ag; for each of these systems, the mechanisms of interaction and damage of both preformed and de novo donor-specific antibodies are reviewed along with their impact on renal graft survival. Moreover, the rejection process can force injured EC to expose cryptic self-Ag, toward which an autoimmune response mounts, overlapping to the allo-immune response in the damaging of the graft. Not only are EC a passive target of the host immune response but also an active player in lymphocyte activation; therefore, their interaction with allogenic T-cells is analyzed on the basis of experimental in vitro and in vivo studies, according to the patterns of expression of the HLA class I and II and the co-stimulatory molecules specific for cytotoxic and helper T-cells. Finally, as the response that follows transplantation has proven to be not necessarily destructive, the factors that foster graft endothelium functioning in spite of rejection, and how they could be therapeutically harnessed to promote long-term graft acceptance, are described: accommodation that is resistance of EC to donor-specific antibodies, and endothelial cell ability to induce Foxp3+ regulatory T-cells, that are crucial mediators of tolerance.
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Em] Entry month:1411
[Da] Date of entry for processing:141106
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.3389/fimmu.2014.00505

  9 / 444588 MEDLINE  
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[PMID]: 25374665
[Au] Autor:Watzl C; Urlaub D; Fasbender F; Claus M
[Ad] Address:IfADo - Leibniz Research Centre for Working Environment and Human Factors Ardeystrasse 67, 44139 Dortmund Germany....
[Ti] Title:Natural killer cell regulation - beyond the receptors.
[So] Source:F1000Prime Rep;6:87, 2014.
[Is] ISSN:2051-7599
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Natural killer (NK) cells are lymphocytes that are important for early and effective immune responses against infections and cancer. In the last 40 years, many receptors, their corresponding ligands and signaling pathways that regulate NK cell functions have been identified. However, we now know that additional processes, such as NK cell education, differentiation and also the formation of NK cell memory, have a great impact on the reactivity of these cells. Here, we summarize the current knowledge about these modulatory processes.
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Em] Entry month:1411
[Da] Date of entry for processing:141106
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.12703/P6-87

  10 / 444588 MEDLINE  
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[PMID]: 25310240
[Au] Autor:Yu X; Wang Y; Deng M; Li Y; Ruhn KA; Zhang CC; Hooper LV
[Ad] Address:Department of Immunology, University of Texas Southwestern Medical Center, Dallas, United States....
[Ti] Title:The basic leucine zipper transcription factor NFIL3 directs the development of a common innate lymphoid cell precursor.
[So] Source:Elife;3, 2014.
[Is] ISSN:2050-084X
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Innate lymphoid cells (ILCs) are recently identified lymphocytes that limit infection and promote tissue repair at mucosal surfaces. However, the pathways underlying ILC development remain unclear. Here we show that the transcription factor NFIL3 directs the development of a committed bone marrow precursor that differentiates into all known ILC lineages. NFIL3 was required in the common lymphoid progenitor (CLP), and was essential for the differentiation of αLP, a bone marrow cell population that gives rise to all known ILC lineages. Clonal differentiation studies revealed that CXCR6(+) cells within the αLP population differentiate into all ILC lineages but not T- and B-cells. We further show that NFIL3 governs ILC development by directly regulating expression of the transcription factor TOX. These findings establish that NFIL3 directs the differentiation of a committed ILC precursor that gives rise to all ILC lineages and provide insight into the defining role of NFIL3 in ILC development.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1411
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.7554/eLife.04406


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