Database : MEDLINE
Search on : Melioidosis [Words]
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[PMID]: 29474381
[Au] Autor:Sengyee S; Yoon SH; Paksanont S; Yimthin T; Wuthiekanun V; Limmathurotsakul D; West TE; Ernst RK; Chantratita N
[Ad] Address:Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
[Ti] Title:Comprehensive analysis of clinical Burkholderia pseudomallei isolates demonstrates conservation of unique lipid A structure and TLR4-dependent innate immune activation.
[So] Source:PLoS Negl Trop Dis;12(2):e0006287, 2018 Feb.
[Is] ISSN:1935-2735
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Burkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high mortality rates. Lipopolysaccharide (LPS) of B. pseudomallei is a potent immunostimulatory molecule comprised of lipid A, core, and O-polysaccharide (OPS) components. Four B. pseudomallei LPS types have been described based on SDS-PAGE patterns that represent the difference of OPS-type A, type B, type B2 and rough LPS. The majority of B. pseudomallei isolates are type A. We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) followed by electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QqTOF MS) and gas chromatography to characterize the lipid A of B. pseudomallei within LPS type A isolates. We determined that B. pseudomallei lipid A is represented by penta- and tetra-acylated species modified with 4-amino-4-deoxy-arabinose (Ara4N). The MALDI-TOF profiles from 171 clinical B. pseudomallei isolates, including 68 paired primary and relapse isolates and 35 within-host isolates were similar. We did not observe lipid A structural changes when the bacteria were cultured in different growth conditions. Dose-dependent NF-κB activation in HEK cells expressing TLR4 was observed using multiple heat-killed B. pseudomallei isolates and corresponding purified LPS. We demonstrated that TLR4-dependent NF-κB activation induced by heat-killed bacteria or LPS prepared from OPS deficient mutant was significantly greater than those induced by wild type B. pseudomallei. These findings suggest that the structure of B. pseudomallei lipid A is highly conserved in a wide variety of clinical and environmental circumstances but that the presence of OPS may modulate LPS-driven innate immune responses in melioidosis.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[St] Status:In-Data-Review
[do] DOI:10.1371/journal.pntd.0006287

  2 / 2492 MEDLINE  
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[PMID]: 29394337
[Au] Autor:Sarovich DS; Webb JR; Pitman MC; Viberg LT; Mayo M; Baird RW; Robson JM; Currie BJ; Price EP
[Ad] Address:Global and Tropical Health Division, Menzies School of Health Research, Tiwi, Northern Territory, Australia.
[Ti] Title:Raising the stakes: Loss of efflux-pump regulation decreases meropenem susceptibility in Burkholderia pseudomallei.
[So] Source:Clin Infect Dis;, 2018 Jan 31.
[Is] ISSN:1537-6591
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Background: Burkholderia pseudomallei, the causative agent of the high-mortality disease melioidosis, is a Gram-negative bacterium that is naturally resistant to many antibiotics. There is no vaccine for melioidosis, and effective eradication is reliant on biphasic and prolonged antibiotic administration. The carbapenem drug, meropenem, is the current gold-standard option for treating severe melioidosis. Intrinsic B. pseudomallei resistance towards meropenem has not yet been documented; however, resistance could conceivably develop over the course of infection, leading to prolonged sepsis and treatment failure. Methods: We examined our 30-year clinical collection of melioidosis cases to identify B. pseudomallei isolates with reduced meropenem susceptibility. Isolates were subjected to minimum inhibitory concentration (MIC) testing towards meropenem. Paired isolates from patients that had evolved decreased susceptibility were subjected to whole-genome sequencing. Select Agent-compliant genetic manipulation was carried out to confirm the molecular mechanisms conferring resistance. Results: We identified 11 melioidosis cases where B. pseudomallei isolates developed decreased susceptibility towards meropenem during treatment, including two cases not treated with this antibiotic. Meropenem MICs increased from 0.5-0.75 to 3-8 µg/mL. Comparative genomics identified multiple mutations affecting multidrug resistance-nodulation-division (RND) efflux pump regulators, with concomitant over-expression of their corresponding pumps. All cases were refractory to treatment despite aggressive, targeted therapy, and two were associated with a fatal outcome. Conclusions: This study confirms the role of RND efflux pumps in decreased meropenem susceptibility in B. pseudomallei. These findings have important ramifications for the diagnosis, treatment and management of life-threatening melioidosis cases.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[St] Status:Publisher
[do] DOI:10.1093/cid/ciy069

  3 / 2492 MEDLINE  
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[PMID]: 29363975
[Au] Autor:Ruiz SI; Bowen LE; Bailey MM; Berkland C
[Ad] Address:United States Army Medical Research Institute of Infectious Diseases , Fort Detrick , Maryland 21702 , United States.
[Ti] Title:Pulmonary Delivery of Ceftazidime for the Treatment of Melioidosis in a Murine Model.
[So] Source:Mol Pharm;15(3):1371-1376, 2018 Mar 05.
[Is] ISSN:1543-8392
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Burkholderia pseudomallei, the etiological agent responsible for melioidosis, exhibits a great public health toll in its endemic regions. The elevation of B. pseudomallei to a Tier I select agent underscores the urgent need for effective therapeutics and preventatives. The current treatment regimen for melioidosis is suboptimal, requiring an intensive phase of intravenous antibiotic followed by months of oral antibiotics. Inhaled antibiotics are a promising avenue to pursue for pulmonary diseases, including melioidosis, since this mode of delivery mimics the likely exposure route and can provide high drug doses directly to the infected tissue. Ceftazidime was delivered via a nose-only system to BALB/c mice challenged with B. pseudomallei. Mice treated with nebulized ceftazidime became symptomatic but survived until study end, which was comparable to those treated intraperitoneally. Upon necropsy, bacteria remained within the spleens of the majority of the experimental animals. The effectiveness of nebulized ceftazidime warrants additional studies to improve the treatment regimen and to test as a prophylactic therapy against B. pseudomallei.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180305
[Lr] Last revision date:180305
[St] Status:In-Data-Review
[do] DOI:10.1021/acs.molpharmaceut.7b00938

  4 / 2492 MEDLINE  
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[PMID]: 29208262
[Au] Autor:Chou DW; Lin YW
[Ad] Address:Department of Critical Care Medicine, Tainan Municipal Hospital, Tainan, Taiwan. Electronic address: choudw@gmail.com.
[Ti] Title:Splenic Infarction and Rupture Due to Melioidosis.
[So] Source:Am J Med Sci;354(6):633-634, 2017 12.
[Is] ISSN:1538-2990
[Cp] Country of publication:United States
[La] Language:eng
[Mh] MeSH terms primary: Melioidosis/diagnostic imaging
Rupture, Spontaneous/diagnostic imaging
Splenic Infarction/diagnostic imaging
Splenic Rupture/diagnostic imaging
[Mh] MeSH terms secundary: Diabetes Complications/diagnostic imaging
Female
Humans
Melioidosis/complications
Middle Aged
Rupture, Spontaneous/etiology
Splenic Infarction/etiology
Splenic Rupture/etiology
[Pt] Publication type:CASE REPORTS; JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180301
[Lr] Last revision date:180301
[Js] Journal subset:AIM; IM
[Da] Date of entry for processing:171207
[St] Status:MEDLINE

  5 / 2492 MEDLINE  
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[PMID]: 29364892
[Au] Autor:Hantrakun V; Thaipadungpanit J; Rongkard P; Srilohasin P; Amornchai P; Langla S; Mukaka M; Chantratita N; Wuthiekanun V; Dance DAB; Day NPJ; Peacock SJ; Limmathurotsakul D
[Ad] Address:Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
[Ti] Title:Presence of B. thailandensis and B. thailandensis expressing B. pseudomallei-like capsular polysaccharide in Thailand, and their associations with serological response to B. pseudomallei.
[So] Source:PLoS Negl Trop Dis;12(1):e0006193, 2018 Jan.
[Is] ISSN:1935-2735
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Burkholderia pseudomallei is an environmental Gram-negative bacillus and the cause of melioidosis. B. thailandensis, some strains of which express a B. pseudomallei-like capsular polysaccharide (BTCV), is also commonly found in the environment in Southeast Asia but is considered non-pathogenic. The aim of the study was to determine the distribution of B. thailandensis and its capsular variant in Thailand and investigate whether its presence is associated with a serological response to B. pseudomallei. METHODOLOGY/PRINCIPAL FINDINGS: We evaluated the presence of B. pseudomallei and B. thailandensis in 61 rice fields in Northeast (n = 21), East (n = 19) and Central (n = 21) Thailand. We found BTCV in rice fields in East and Central but not Northeast Thailand. Fourteen fields were culture positive for B. pseudomallei alone, 8 for B. thailandensis alone, 11 for both B. pseudomallei and B. thailandensis, 6 for both B. thailandensis and BTCV, and 5 for B. pseudomallei, B. thailandensis and BTCV. Serological testing using the indirect hemagglutination assay (IHA) of 96 farmers who worked in the study fields demonstrated that farmers who worked in B. pseudomallei-positive fields had higher IHA titers than those who worked in B. pseudomallei-negative fields (median 1:40 [range: <1:10-1:640] vs. <1:10 [range: <1:10-1:320], p = 0.002). In a multivariable ordered logistic regression model, IHA titers were significantly associated with the presence of B. pseudomallei (aOR = 3.7; 95% CI 1.8-7.8, p = 0.001) but were not associated with presence of B. thailandensis (p = 0.32) or BTCV (p = 0.32). One sequence type (696) was identified for the 27 BTCV isolates tested. CONCLUSIONS/SIGNIFICANCE: This is the first report of BTCV in Thailand. The presence of B. pseudomallei and B. thailandensis in the same field was not uncommon. Our findings suggest that IHA positivity of healthy rice farmers in Thailand is associated with the presence of B. pseudomallei in rice fields rather than B. thailandensis or BTCV.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180301
[Lr] Last revision date:180301
[St] Status:In-Data-Review
[do] DOI:10.1371/journal.pntd.0006193

  6 / 2492 MEDLINE  
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[PMID]: 29315931
[Au] Autor:García-Jiménez WL; Salguero FJ; D'Elia RV
[Ad] Address:Department of Pathology and Infectious Diseases, School of Veterinary Medicine, University of Surrey, Guildford, UK.
[Ti] Title:Histopathological and immunohistochemical characterization of Burkholderia pseudomallei lesions in an acute model of infection with BALB/c mice.
[So] Source:Int J Exp Pathol;98(6):347-355, 2017 Dec.
[Is] ISSN:1365-2613
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Organ tissue damage is a key contributor to host morbidity and mortality following infection with microbial agents. Severe immune responses, excessive cellular recruitment and necrosis of cells all play a role in disease pathology. Understanding the pathogenesis of disease can aid in identifying potential new therapeutic targets or simply act as a diagnostic tool. Burkholderia pseudomallei is a Gram-negative bacterium that can cause acute and chronic diseases. The BALB/c mouse has been shown to be highly susceptible to aerosol challenge with B. pseudomallei and hence acts as a good model to study the acute and potentially lethal form of the disease melioidosis. In our study, BALB/c mice were challenged and culled at predetermined time points to generate a pathological time course of infection. Lung, liver and spleen were subjected to pathological and immunohistochemical analysis. The number and type of microscopic lesions within each organ, as well as the location and the mean percentage of neutrophils, B cells, T cells and Burkholderia capsule antigen within the lesions, were all characterized during the time course. Neutrophils were determined as the key player in tissue pathology and generation of lesions, with B cells playing an insignificant role. This detailed pathological assessment increases our understanding of B. pseudomallei disease.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180301
[Lr] Last revision date:180301
[St] Status:In-Data-Review
[do] DOI:10.1111/iep.12258

  7 / 2492 MEDLINE  
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[PMID]: 29483111
[Au] Autor:Chan E; Martelli P; Hui SW; Teng JLL; Lau SKP; Woo PCY
[Ad] Address:Department of Microbiology, The University of Hong Kong, Hong Kong, China.
[Ti] Title: susceptibility of ceftolozane-tazobactam against .
[So] Source:Antimicrob Agents Chemother;, 2018 Feb 26.
[Is] ISSN:1098-6596
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:is the cause of melioidosis, a potentially serious and fatal disease characterized by community-acquired pneumonia and/or sepsis mainly in Southeast Asia and Northern Australia, and high case-fatality rates of up to 19% are observed in endemic areas (1).
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180227
[Lr] Last revision date:180227
[St] Status:Publisher

  8 / 2492 MEDLINE  
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[PMID]: 29462444
[Au] Autor:Chen LH; Leder K; Barbre KA; Schlagenhauf P; Libman M; Keystone J; Mendelson M; Gautret P; Schwartz E; Shaw M; MacDonald S; McCarthy A; Connor BA; Esposito DH; Hamer D; Wilson ME; GeoSentinel Surveillance Network
[Ad] Address:Mount Auburn Hospital, Cambridge, Massachusetts, and Harvard Medical School, Boston, MA, USA.
[Ti] Title:Business travel-associated illness: a GeoSentinel analysis.
[So] Source:J Travel Med;25(1), 2018 Jan 01.
[Is] ISSN:1708-8305
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Background: Analysis of a large cohort of business travelers will help clinicians focus on frequent and serious illnesses. We aimed to describe travel-related health problems in business travelers. Methods: GeoSentinel Surveillance Network consists of 64 travel and tropical medicine clinics in 29 countries; descriptive analysis was performed on ill business travelers, defined as persons traveling for work, evaluated after international travel 1 January 1997 through 31 December 2014. Results: Among 12 203 business travelers seen 1997-2014 (14 045 eligible diagnoses), the majority (97%) were adults aged 20-64 years; most (74%) reported from Western Europe or North America; two-thirds were male. Most (86%) were outpatients. Fewer than half (45%) reported a pre-travel healthcare encounter. Frequent regions of exposure were sub-Saharan Africa (37%), Southeast Asia (15%) and South Central Asia (14%). The most frequent diagnoses were malaria (9%), acute unspecified diarrhea (8%), viral syndrome (6%), acute bacterial diarrhea (5%) and chronic diarrhea (4%). Species was reported for 973 (90%) of 1079 patients with malaria, predominantly Plasmodium falciparum acquired in sub-Saharan Africa. Of 584 (54%) with malaria chemoprophylaxis information, 92% took none or incomplete courses. Thirteen deaths were reported, over half of which were due to malaria; others succumbed to pneumonia, typhoid fever, rabies, melioidosis and pyogenic abscess. Conclusions: Diarrheal illness was a major cause of morbidity. Malaria contributed substantial morbidity and mortality, particularly among business travelers to sub-Saharan Africa. Underuse or non-use of chemoprophylaxis contributed to malaria cases. Deaths in business travelers could be reduced by improving adherence to malaria chemoprophylaxis and targeted vaccination for vaccine-preventable diseases. Pre-travel advice is indicated for business travelers and is currently under-utilized and needs improvement.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180227
[Lr] Last revision date:180227
[St] Status:In-Data-Review
[do] DOI:10.1093/jtm/tax097

  9 / 2492 MEDLINE  
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[PMID]: 29368689
[Au] Autor:Nielsen AD; Ellekvist P
[Ad] Address:ariella.nielsen@gmail.com.
[Ti] Title:[Melioidosis is a rare diagnosis among returning travellers with fever].
[So] Source:Ugeskr Laeger;180(2), 2018 01 22.
[Is] ISSN:1603-6824
[Cp] Country of publication:Denmark
[La] Language:dan
[Ab] Abstract:The causative organism of melioidosis is Burkholderia pseudomallei, a Gram-negative bacterium endemic to Southeast Asia and Northern Australia. The clinical presentation varies, ranging from asymptomatic cases to rapidly progressive septicaemia and shock. Abscesses may be found in any internal organ. We describe an infrequent presentation of melioidosis with various symptoms, including haematuria and rectal pain. Perirectal and prostatic abscesses were found, the causative organism was identified and relevant treatment administered, resulting in a full recovery.
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180227
[Lr] Last revision date:180227
[St] Status:In-Process

  10 / 2492 MEDLINE  
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[PMID]: 29393577
[Au] Autor:Mariappan V; Thimma J; Vellasamy KM; Shankar EM; Vadivelu J
[Ad] Address:Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
[Ti] Title:Adhesion and invasion attributes of Burkholderia pseudomallei are dependent on airway surface liquid and glucose concentrations in lung epithelial cells.
[So] Source:Environ Microbiol Rep;, 2018 Feb 02.
[Is] ISSN:1758-2229
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Physiological constituents in airway surface liquids (ASL) appear to impact the adherence and invasion potentials of Burkholderia pseudomallei contributing to recrudescent melioidosis. Here, we investigated the factors present in ASL that is likely to influence bacterial adhesion and invasion leading to improved understanding of bacterial pathogenesis. Six B. pseudomallei clinical isolates from different origins were used to investigate the ability of the bacteria to adhere and invade A549 human lung epithelial cells using a system that mimics the physiological ASL with different pH, NaCl, KCl, CaCl and glucose concentrations. These parameters resulted in markedly differential adherence and invasion abilities of B. pseudomallei to the lung epithelial cells. The concentration of 20 mM glucose dramatically increased adherence and invasion by increasing the rate of pili formation in depiliated bacteria. Glucose significantly increased adherence and invasion of B. pseudomallei to A549 cells, and presence of NaCl, KCl and CaCl markedly ablated the effect despite the presence of glucose. Our data established a link between glucose, enhanced adhesion and invasion potentials of B. pseudomallei, hinting increased susceptibility of individuals with diabetes mellitus to clinical melioidosis.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180223
[Lr] Last revision date:180223
[St] Status:Publisher
[do] DOI:10.1111/1758-2229.12624


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