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[PMID]: 28381682
[Au] Autor:Fu Y; Shi T; Xu L; Wei W; Lu F; Zhang X; Yuan X; Li J; Lv J; Fang W
[Ad] Address:Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China.
[Ti] Title:Identification of a novel Hemoplasma species from pigs in Zhejiang province, China.
[So] Source:J Vet Med Sci;79(5):864-870, 2017 May 18.
[Is] ISSN:1347-7439
[Cp] Country of publication:Japan
[La] Language:eng
[Ab] Abstract:Hemoplasmas belong to Mycoplasmataceae (Mollicutes: Mycoplasmatales) and are able to infect a broad range of mammalian species. We investigated prevalence of hemotropic mycoplasma species in pig farms in the region of Zhejiang by a PCR scheme using universal primers targeting 16S rRNA and RNase P RNA gene (rnpB). Representative positive samples from different farms were selected for sequencing of 16S rRNA and the 219bp rnpB gene fragments for phylogenetic analysis. Sequencing analysis of PCR products from first samples identified a novel hemoplasma species present in several pig farms in the region with highest nucleotide identity of 92% to Candidatus Mycoplasma turicensis. A duplex PCR assay was then designed for differential detection of the novel hemoplasma from Mycoplasma parvum/M. suis in field samples. Of 324 blood samples from clinically healthy pigs, 26.5% was positive for this novel hemoplasma species and 50% positive for M. suis/M. parvum, indicating that the novel hemotropic mycoplasma species were of considerably high prevalence in Zhejiang province, China.
[Mh] MeSH terms primary: Mycoplasmataceae/isolation & purification
Mycoplasmatales Infections/veterinary
Swine Diseases/microbiology
[Mh] MeSH terms secundary: Animals
China
Mycoplasmataceae/classification
Mycoplasmatales Infections/microbiology
Phylogeny
Polymerase Chain Reaction/veterinary
RNA, Ribosomal, 16S
Swine
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (RNA, Ribosomal, 16S)
[Em] Entry month:1710
[Cu] Class update date: 171031
[Lr] Last revision date:171031
[Js] Journal subset:IM
[Da] Date of entry for processing:170407
[St] Status:MEDLINE
[do] DOI:10.1292/jvms.16-0545

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[PMID]: 25892209
[Au] Autor:Takanashi M; Ito S; Kaneto H; Tanahashi Y; Kitanohara M; Yanagihara A; Nakazima H; Yasuda M
[Ad] Address:Infectious Disease Testing Department, LSI Medience Corporation, Tokyo, Japan. Electronic address: takanashi.masaki@ms.medience.co.jp.
[Ti] Title:Development and clinical application of an InvaderPlus® assay for the detection of genital mycoplasmas.
[So] Source:J Infect Chemother;21(7):516-9, 2015 Jul.
[Is] ISSN:1437-7780
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:We developed a PCR-based assay involving Invader® technology for detection of the genital mycoplasmas of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum. We compared its performance with that of a PCR-microtiter plate hybridization assay, which we developed previously, in detecting genital mycoplasmas in first-voided urine (FVU) specimens from men with non-gonococcal urethritis. The tests targeting each of the genital mycoplasmas were specific for the respective species and could detect as few as 10 copies of the plasmids containing the target genes of each of the genital mycoplasmas per reaction. The assay using the InvaderPlus® method (InvaderPlus® assay) showed very similar performance to that of the PCR-microtiter plate hybridization assay for detecting the genital mycoplasmas in the FVU specimens. In addition, the PCR and endonuclease reaction in the InvaderPlus® assay were carried out simultaneously in one procedure, thus simplifying the assay, leading to time- and labor-savings and a decrease in the risk of specimen contamination. The InvaderPlus® assay could be useful in diagnosing genitourinary tract infections caused by the genital mycoplasmas.
[Mh] MeSH terms primary: Male Urogenital Diseases/microbiology
Molecular Typing/methods
Mycoplasmataceae/genetics
Mycoplasmatales Infections/microbiology
[Mh] MeSH terms secundary: DNA, Bacterial/analysis
DNA, Bacterial/genetics
Humans
Male
Male Urogenital Diseases/diagnosis
Mycoplasmatales Infections/diagnosis
Polymerase Chain Reaction/methods
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Bacterial)
[Em] Entry month:1603
[Cu] Class update date: 150615
[Lr] Last revision date:150615
[Js] Journal subset:IM
[Da] Date of entry for processing:150421
[St] Status:MEDLINE

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[PMID]: 24849820
[Au] Autor:Vouga M; Greub G; Prod'hom G; Durussel C; Roth-Kleiner M; Vasilevsky S; Baud D
[Ad] Address:Materno-fetal and Obstetrics Research Unit, Department of Obstetrics and Gynaecology, Maternity, University Hospital, Lausanne, Switzerland.
[Ti] Title:Treatment of genital mycoplasma in colonized pregnant women in late pregnancy is associated with a lower rate of premature labour and neonatal complications.
[So] Source:Clin Microbiol Infect;20(10):1074-9, 2014 Oct.
[Is] ISSN:1469-0691
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Mycoplasma hominis and Ureaplasma spp. may colonize the human genital tract and have been associated with adverse pregnancy outcomes such as preterm labour and preterm premature rupture of membranes. However, as these bacteria can reside in the normal vaginal flora, there are controversies regarding their true role during pregnancy and so the need to treat these organisms. We therefore conducted a retrospective analysis to evaluate the treatment of genital mycoplasma in 5377 pregnant patients showing symptoms of potential obstetric complications at 25-37 weeks of gestation. Women presenting with symptoms were routinely screened by culture for the presence of these bacteria and treated with clindamycin when positive. Compared with uninfected untreated patients, women treated for genital mycoplasma demonstrated lower rates of premature labour. Indeed preterm birth rates were, respectively, 40.9% and 37.7% in women colonized with Ureaplasma spp. and M. hominis, compared with 44.1% in uncolonized women (Ureaplasma spp., p 0.024; M. hominis, p 0.001). Moreover, a reduction of neonatal complications rates was observed, with 10.9% of newborns developing respiratory diseases in case of Ureaplasma spp. colonization and 5.9% in the presence of M. hominis, compared with 12.8% in the absence of those bacteria (Ureaplasma spp., p 0.050; M. hominis, p <0.001). Microbiological screening of Ureaplasma spp. and/or M. hominis and pre-emptive antibiotic therapy of symptomatic pregnant women in late pregnancy might represent a beneficial strategy to reduce premature labour and neonatal complications.
[Mh] MeSH terms primary: Anti-Bacterial Agents/therapeutic use
Clindamycin/therapeutic use
Mycoplasmatales Infections/drug therapy
Pregnancy Complications, Infectious/drug therapy
Premature Birth/prevention & control
Reproductive Tract Infections/drug therapy
Respiratory Distress Syndrome, Newborn/prevention & control
[Mh] MeSH terms secundary: Adult
Female
Humans
Infant, Newborn
Middle Aged
Mycoplasma/growth & development
Mycoplasmatales Infections/microbiology
Pregnancy
Pregnancy Complications, Infectious/microbiology
Pregnancy Outcome
Premature Birth/epidemiology
Premature Birth/etiology
Reproductive Tract Infections/microbiology
Respiratory Distress Syndrome, Newborn/complications
Respiratory Distress Syndrome, Newborn/epidemiology
Respiratory Distress Syndrome, Newborn/etiology
Retrospective Studies
Ureaplasma/growth & development
Young Adult
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Anti-Bacterial Agents); 3U02EL437C (Clindamycin)
[Em] Entry month:1507
[Cu] Class update date: 141203
[Lr] Last revision date:141203
[Js] Journal subset:IM
[Da] Date of entry for processing:140523
[St] Status:MEDLINE
[do] DOI:10.1111/1469-0691.12686

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[PMID]: 24679107
[Au] Autor:Redelinghuys MJ; Ehlers MM; Dreyer AW; Lombaard HA; Kock MM
[Ad] Address:Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa. shanered72@gmail.com.
[Ti] Title:Antimicrobial susceptibility patterns of Ureaplasma species and Mycoplasma hominis in pregnant women.
[So] Source:BMC Infect Dis;14:171, 2014 Mar 28.
[Is] ISSN:1471-2334
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: Genital mycoplasmas colonise up to 80% of sexually mature women and may invade the amniotic cavity during pregnancy and cause complications. Tetracyclines and fluoroquinolones are contraindicated in pregnancy and erythromycin is often used to treat patients. However, increasing resistance to common antimicrobial agents is widely reported. The purpose of this study was to investigate antimicrobial susceptibility patterns of genital mycoplasmas in pregnant women. METHODS: Self-collected vaginal swabs were obtained from 96 pregnant women attending an antenatal clinic in Gauteng, South Africa. Specimens were screened with the Mycofast Revolution assay for the presence of Ureaplasma species and Mycoplasma hominis. The antimicrobial susceptibility to levofloxacin, moxifloxacin, erythromycin, clindamycin and tetracycline were determined at various breakpoints. A multiplex polymerase chain reaction assay was used to speciate Ureaplasma positive specimens as either U. parvum or U. urealyticum. RESULTS: Seventy-six percent (73/96) of specimens contained Ureaplasma spp., while 39.7% (29/73) of Ureaplasma positive specimens were also positive for M. hominis. Susceptibilities of Ureaplasma spp. to levofloxacin and moxifloxacin were 59% (26/44) and 98% (43/44) respectively. Mixed isolates (Ureaplasma species and M. hominis) were highly resistant to erythromycin and tetracycline (both 97% resistance). Resistance of Ureaplasma spp. to erythromycin was 80% (35/44) and tetracycline resistance was detected in 73% (32/44) of Ureaplasma spp. Speciation indicated that U. parvum was the predominant Ureaplasma spp. conferring antimicrobial resistance. CONCLUSIONS: Treatment options for genital mycoplasma infections are becoming limited. More elaborative studies are needed to elucidate the diverse antimicrobial susceptibility patterns found in this study when compared to similar studies. To prevent complications in pregnant women, the foetus and the neonate, routine screening for the presence of genital mycoplasmas is recommended. In addition, it is recommended that antimicrobial susceptibility patterns are determined.
[Mh] MeSH terms primary: Anti-Bacterial Agents/pharmacology
Mycoplasma hominis/drug effects
Mycoplasma hominis/isolation & purification
Mycoplasmatales Infections/microbiology
Pregnancy Complications, Infectious/microbiology
Ureaplasma/drug effects
Ureaplasma/isolation & purification
[Mh] MeSH terms secundary: Adult
Female
Humans
Microbial Sensitivity Tests
Pregnancy
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Anti-Bacterial Agents)
[Em] Entry month:1501
[Cu] Class update date: 170220
[Lr] Last revision date:170220
[Js] Journal subset:IM
[Da] Date of entry for processing:140401
[St] Status:MEDLINE
[do] DOI:10.1186/1471-2334-14-171

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Timenetsky, Jorge
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[PMID]: 23677636
[Au] Autor:Neto RL; Marques LM; Guimarães AM; Yamaguti M; Oliveira RC; Gaetti-Jardim E; Medina AO; Sanfilippo LF; Timenetsky J
[Ad] Address:Instituto de Ciências Biomédicas II, Universidade de São Paulo, Rua Professor Lineu Prestes, São Paulo, SP, Brazil.
[Ti] Title:Frequency of different human mollicutes species in the mucosa of the oropharynx, conjunctiva, and genitalia of free-ranging and captive capuchin monkeys (Cebus spp.).
[So] Source:Am J Primatol;75(10):973-8, 2013 Oct.
[Is] ISSN:1098-2345
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:This study is the first to evaluate the occurrence of several Mollicutes species in Brazilian capuchin monkeys (Cebus spp.). Mollicutes were detected by culture and polymerase chain reaction (PCR) in samples of the oropharyngeal, conjuctiva, and genital mucosae of 58 monkeys. In the oropharynx, Mollicutes in general (generic PCR to the Class), and those of the genus Ureaplasma (genus PCR), were detected in 72.4% and 43.0% of the samples, respectively. The identified species in this site included: Mycoplasma arginini (43.1%), M. salivarium (41.4%), and M. pneumoniae (19.0%). Both Ureaplasma and Mycoplasma are genera of the order Mycoplasmatales. In the preputial/vaginal mucosa, PCR detected Mollicutes in general in 27.58% of the samples, the genus Ureaplasma in 32.7%, the species M. arginini in 8.6%, and Acholeplasma laidlawii of the order Acholeplasmatales in 1.7% In the conjunctiva, Mollicutes in general were detected in 29.3% of the samples, with 1.7% being identified as A. laidlawii. Culturing was difficult due to contamination, but two isolates were successfully obtained. The Mollicutes species of this study provided new insights into these bacteria in Brazilian Cebus. Studies are lacking of the actual risk of Mollicutes infection or the frequency at which primates serve as permanent or temporary reservoirs for Mollicutes. In the present study, the samples were collected from monkeys without clinical signs of infection. The mere presence of Mollicutes, particularly those also found in humans, nevertheless signals a need for studies to evaluate the impact of these microorganisms on the health of non-human primates (NHPs) and the possibility of cross-species transmission between NHPs and humans.
[Mh] MeSH terms primary: Cebus/microbiology
Tenericutes/isolation & purification
Zoonoses/microbiology
[Mh] MeSH terms secundary: Animals
Brazil/epidemiology
Conjunctiva/microbiology
DNA, Bacterial/chemistry
DNA, Bacterial/genetics
Female
Genitalia/microbiology
Humans
Male
Oropharynx/microbiology
Polymerase Chain Reaction/veterinary
RNA, Ribosomal, 16S/chemistry
RNA, Ribosomal, 16S/genetics
Statistics, Nonparametric
Tenericutes/genetics
Zoonoses/epidemiology
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Entry month:1403
[Cu] Class update date: 130821
[Lr] Last revision date:130821
[Js] Journal subset:IM
[Da] Date of entry for processing:130517
[St] Status:MEDLINE
[do] DOI:10.1002/ajp.22164

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[PMID]: 22930310
[Au] Autor:Wang B; Wu JR; Guo HJ; Yang HT; Ai J; Hui M; Chan CY
[Ad] Address:School of Public Health, Southeast University, Nanjing, Jiangsu 210009, People's Republic of China. wangbeilxb@seu.edu.cn
[Ti] Title:The prevalence of six species of Mycoplasmataceae in an HIV/AIDS population in Jiangsu Province, China.
[So] Source:Int J STD AIDS;23(8):e7-10, 2012 Aug.
[Is] ISSN:1758-1052
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:This study employed culture and polymerase chain reaction (PCR) to examine the prevalence of Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium, Mycoplasma fermentans, Mycoplasma penetrans and Mycoplasma pirum in 210 HIV/AIDS patients, 455 sexually transmitted infection (STI) clinic attendees and 245 healthy volunteers from first-void urine specimens for men and endocervical swabs for women. U. urealyticum and M. hominis were detected in 107 (51.0%) and 69 (32.9%) patients in the HIV/AIDS group. At least one of the other four organisms was detected in 34 (16.2%) HIV/AIDS patients, 29 (6.4%) STI clinic attendees and six (2.5%) healthy volunteers. This study showed that U. urealyticum, M. hominis and M. fermentans were significantly more prevalent in HIV/AIDS patients, as were other mycoplasmas. Our results suggest a possible role for co-infection.
[Mh] MeSH terms primary: AIDS-Related Opportunistic Infections/epidemiology
Mycoplasmataceae
Mycoplasmatales Infections/epidemiology
[Mh] MeSH terms secundary: Adolescent
Adult
Aged
Child
Child, Preschool
China/epidemiology
Female
Humans
Male
Middle Aged
Mycoplasma
Mycoplasma Infections/epidemiology
Mycoplasma Infections/microbiology
Mycoplasma fermentans
Mycoplasma genitalium
Mycoplasma hominis
Mycoplasma penetrans
Mycoplasmatales Infections/microbiology
Polymerase Chain Reaction
Prevalence
Ureaplasma Infections/epidemiology
Ureaplasma Infections/microbiology
Ureaplasma urealyticum
Young Adult
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1211
[Cu] Class update date: 120829
[Lr] Last revision date:120829
[Js] Journal subset:IM; X
[Da] Date of entry for processing:120830
[St] Status:MEDLINE
[do] DOI:10.1258/ijsa.2009.009396

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[PMID]: 22252268
[Au] Autor:Lee SJ; Park DC; Lee DS; Choe HS; Cho YH
[Ad] Address:Department of Urology, St. Vincent's Hospital, The Catholic University of Korea College of Medicine, Suwon, Korea.
[Ti] Title:Evaluation of Seeplex® STD6 ACE Detection kit for the diagnosis of six bacterial sexually transmitted infections.
[So] Source:J Infect Chemother;18(4):494-500, 2012 Aug.
[Is] ISSN:1437-7780
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Traditionally, the diagnosis of bacterial sexually transmitted infection (STI) has been dependent on the isolation of the causative pathogens by culturing endocervical or urethral swab specimens on selective media. While such procedures typically provide excellent diagnostic accuracy, they are often time-consuming and expensive. A multiplex polymerase chain reaction (PCR) assay, based on a semi-automated detection system, was evaluated for the detection of six STI causative organisms. The Seeplex(®) STD6 ACE (auto-capillary electrophoresis) Detection assay employed six pairs of dual priming oligonucleotide (DPO™) primers specifically targeted to unique genes of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Ureaplasma urealyticum, Mycoplasma hominis, and Trichomonas vaginalis. A total of 739 specimens (304 cervical swabs and 435 urine samples) collected for 4 months were tested, and results were compared to those obtained with a combined monoplex PCR. The concordance between the multiplex PCR and monoplex PCR assay was 100% for both sensitivity and specificity. We also tested for the presence of two pathogenic bacteria (C. trachomatis and N. gonorrhoeae) and compared the results obtained with the multiplex PCR and BD ProbeTec duplex strand displacement amplification (SDA). The results of the multiplex PCR and duplex SDA were 99.7% concordant for C. trachomatis and 100% concordant for N. gonorrhoeae. The multiplex PCR assay using the Seeplex(®) STD6 ACE Detection kit proved to be a novel cost-effective and fast diagnostic tool with high sensitivity and specificity for the simultaneous detection of six STI pathogens.
[Mh] MeSH terms primary: Polymerase Chain Reaction/methods
Reagent Kits, Diagnostic
Sexually Transmitted Diseases/diagnosis
Sexually Transmitted Diseases/microbiology
[Mh] MeSH terms secundary: Adult
Cervix Uteri/microbiology
Cervix Uteri/parasitology
Chlamydia trachomatis/genetics
Chlamydia trachomatis/isolation & purification
DNA Primers/genetics
Female
Humans
Male
Middle Aged
Mycoplasmataceae/genetics
Mycoplasmataceae/isolation & purification
Mycoplasmatales Infections/diagnosis
Mycoplasmatales Infections/microbiology
Neisseria gonorrhoeae/genetics
Neisseria gonorrhoeae/isolation & purification
Sensitivity and Specificity
Sexually Transmitted Diseases/parasitology
Sexually Transmitted Diseases/urine
Trichomonas Vaginitis/diagnosis
Trichomonas Vaginitis/parasitology
Trichomonas vaginalis/genetics
Trichomonas vaginalis/isolation & purification
Urine/microbiology
Urine/parasitology
[Pt] Publication type:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA Primers); 0 (Reagent Kits, Diagnostic)
[Em] Entry month:1303
[Cu] Class update date: 120813
[Lr] Last revision date:120813
[Js] Journal subset:IM
[Da] Date of entry for processing:120119
[St] Status:MEDLINE
[do] DOI:10.1007/s10156-011-0362-7

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[PMID]: 22006584
[Au] Autor:Humburg J; Frei R; Wight E; Troeger C
[Ad] Address:Frauenklinik, Kantonsspital Bruderholz, 4001 Bruderholz, Switzerland. joerg.humburg@ksbh.ch
[Ti] Title:Accuracy of urethral swab and urine analysis for the detection of Mycoplasma hominis and Ureaplasma urealyticum in women with lower urinary tract symptoms.
[So] Source:Arch Gynecol Obstet;285(4):1049-53, 2012 Apr.
[Is] ISSN:1432-0711
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:OBJECTIVES: Our objective was to evaluate and compare the accuracy of urethral swabs and urine specimens in the detection of Mycoplasmas in women with lower urinary tract symptoms (LUTS). METHODS: During a urogynecological work-up, including cystometry, we obtained first-void urine, urethral and vaginal swabs in 207 consecutive women at our urogynecological division. Mycoplasma hominis and Ureaplasma urealyticum as well as other microorganisms were detected by standard culture methods. RESULTS: 131 of 207 women reported LUTS. The other 76 formed the controls. Of 207 women 50 (24.2%) had positive cultures for Mycoplasmas. The prevalence of Mycoplasmas in women with LUTS (30.3%) was statistically significant and higher in the group without LUTS (14.5%) (p = 0.011). The detection of M. hominis was most accurate using urethral swab (Specificity 99.9%, PPV 99.6%) compared to the urine specimen (96%, 75%) and vaginal swab (95.1%, 67%). Similar results could be achieved for U. urealyticum (urethral swab: specificity 98.7%, PPV 96.3%; urine specimen: 86.8%, 72%; vaginal swab: 80.5%, 65.2%). CONCLUSION: In the subgroup of women less than 50 years an (detectable) infection due to Mycoplasma or Ureaplasma leads typically to LUTS with normal filling cystometry, whereas no such findings were relevant for the elderly women.
[Mh] MeSH terms primary: Lower Urinary Tract Symptoms/microbiology
Mycoplasma hominis/isolation & purification
Mycoplasmatales Infections/diagnosis
Ureaplasma urealyticum/isolation & purification
Urethra/microbiology
Urine/microbiology
[Mh] MeSH terms secundary: Adult
Female
Humans
Middle Aged
Mycoplasma Infections/complications
Mycoplasma Infections/diagnosis
Mycoplasmatales Infections/complications
Ureaplasma Infections/complications
Ureaplasma Infections/diagnosis
[Pt] Publication type:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Entry month:1207
[Cu] Class update date: 120314
[Lr] Last revision date:120314
[Js] Journal subset:IM
[Da] Date of entry for processing:111019
[St] Status:MEDLINE
[do] DOI:10.1007/s00404-011-2109-1

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[PMID]: 15070999
[Au] Autor:Stellrecht KA; Woron AM; Mishrik NG; Venezia RA
[Ad] Address:Department of Pathology and Laboratory Medicine, Albany Medical Center, Albany, New York 12208, USA. stellrk@mail.amc.edu
[Ti] Title:Comparison of multiplex PCR assay with culture for detection of genital mycoplasmas.
[So] Source:J Clin Microbiol;42(4):1528-33, 2004 Apr.
[Is] ISSN:0095-1137
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Ureaplasma, spp. Mycoplasma genitalium, and Mycoplasma hominis are associated with infection of the genitourinary tract, reproductive failure, and neonatal morbidity and mortality. We have developed a multiplex PCR for the detection of these Mycoplasmatales in a single amplification reaction. The analytical sensitivities of this assay were 10.8, 10.8, and 8.8 CFU for each organism, respectively. This multiplex PCR was compared to culture on 26 cervical swabs, 2 vaginal swabs, 4 female urine specimens, 49 semen samples, 2 male urine specimens, and 1 nonspecified sample. A total of 21 specimens were culture positive (25%); 17 of these were PCR positive. An additional 11 specimens were PCR positive but culture negative. Of the 21 culture-positive specimens, 17 (81%) grew Ureaplasma spp. and 4 (19%) grew Mycoplasma spp. Of the 28 PCR-positive specimens, Ureaplasma spp. was detected in 23 (82%), M. hominis was detected in 3 (11%), and both were detected in 2 (7%). In a confirmatory analysis, all samples were tested by amplification of a second target of the ureaplasma genome. True-positive cases were defined as a positive result by culture or by both amplification assays. The multiplex PCR detected organisms in 26 of the 30 true-positive specimens, as well as in 2 other specimens. Based on a 36% prevalence of infection, the sensitivity, specificity, and positive and negative predictive values of multiplex PCR analyses were 87, 96, 94, and 93%, respectively. Multiplex PCR offers a rapid, sensitive, and easy method to detect genital mycoplasmas.
[Mh] MeSH terms primary: Genital Diseases, Female/microbiology
Genital Diseases, Male/microbiology
Mycoplasmataceae/isolation & purification
Mycoplasmatales Infections/microbiology
Polymerase Chain Reaction/methods
[Mh] MeSH terms secundary: Culture Media
DNA, Bacterial/analysis
Female
Humans
Male
Mycoplasmataceae/classification
Mycoplasmataceae/genetics
Mycoplasmataceae/growth & development
Predictive Value of Tests
Sensitivity and Specificity
[Pt] Publication type:COMPARATIVE STUDY; EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Name of substance:0 (Culture Media); 0 (DNA, Bacterial)
[Em] Entry month:0407
[Cu] Class update date: 140609
[Lr] Last revision date:140609
[Js] Journal subset:IM
[Da] Date of entry for processing:040409
[St] Status:MEDLINE

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[PMID]: 12931378
[Au] Autor:Jiang Y; Wang JY; Zhang SW
[Ad] Address:Department of Andrology, General Hospital, Panzhihua Steel & Iron (Group) Co., Panzhihua, Sichuan 617023, China.
[Ti] Title:[Clinical application research of feilinjian peroral liquid in the treatment of Chlamydia and Mycoplasma infectious prostatitis].
[So] Source:Zhonghua Nan Ke Xue;9(4):309-11, 315, 2003.
[Is] ISSN:1009-3591
[Cp] Country of publication:China
[La] Language:chi
[Ab] Abstract:OBJECTIVES: To evaluate the validity and security of Feilinjian peroral liquid in the treatment of chlamydia trachomatis (CT), mycoplasma hominis (Mh) and Ureaplasma urealyticum (UU) infectious prostatitis. METHODS: Two hundred and three patients of CT, Mh or UU infectious prostatitis diagnosed by strict orientation examination were randomly divided into two groups, one given feilinjian peroral liquid, and the other azithromycin perorally, for one month as treatment period. RESULTS: The analytical results showed that 54 (42.86%) were clinically fully recovered, 32(32.39%) were significantly effective, and 13 (10.31%) were effective in feilinjian peroral liquid group, with total significantly effective rate 68.25% and total effective rate 78.57%, both obviously higher than those in the control group(50.64% and 57.14% respectively). CONCLUSIONS: Feilinjian peroral liquid is significantly better than azithromycin in ameliorating self-conscious symptoms, symptoms scoring of patients with CT, Mh and UU infection, with little side-effects.
[Mh] MeSH terms primary: Chlamydia Infections/drug therapy
Drugs, Chinese Herbal/therapeutic use
Mycoplasmatales Infections/drug therapy
Phytotherapy
Prostatitis/drug therapy
[Mh] MeSH terms secundary: Adult
Chlamydia trachomatis
Humans
Male
Middle Aged
Mycoplasma hominis
Prostatitis/microbiology
Ureaplasma urealyticum
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Name of substance:0 (Drugs, Chinese Herbal)
[Em] Entry month:0702
[Cu] Class update date: 030822
[Lr] Last revision date:030822
[Js] Journal subset:IM
[Da] Date of entry for processing:030823
[St] Status:MEDLINE


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