Database : MEDLINE
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[PMID]: 29395441
[Au] Autor:Lee E; Jeong K; Lee J; Lee S
[Ad] Address:Department of Pediatrics, Ajou University School of Medicine, Suwon, Republic of Korea.
[Ti] Title:Pine nut allergy in Korean children: Clinical characteristics and diagnostic values of specific IgE against pine nuts.
[So] Source:Allergol Immunopathol (Madr);, 2018 Jan 27.
[Is] ISSN:1578-1267
[Cp] Country of publication:Spain
[La] Language:eng
[Ab] Abstract:BACKGROUND: Hypersensitivity reactions to pine nuts in children have been occasionally encountered recently, although reports on pine nut allergy cases are rare worldwide. The study aimed to feature clinical and laboratory findings pertaining to pine nut allergy in Korean children. METHODS: Forty-two subjects were enrolled through a retrospective review of medical records, from September 2010 to December 2015, at the Department of Pediatrics in Ajou University Hospital. The demographic profiles, clinical characteristics, and laboratory findings were evaluated. RESULTS: Twenty-four patients showed immediate-type reactions after exposure to pine nuts (the allergic group), while the remaining 18 were atopic controls, who exhibited no allergic symptoms (the tolerant group). The median age of the subjects in the allergic group was three years. More than half of the subjects in this group experienced allergic symptoms within 5min, and seven of them experienced anaphylaxis. The median level of pine nut-specific immunoglobulin E (sIgE) in the allergic group (1.62kU /L) was significantly higher (p=0.014) than that in the tolerant group (0.11kU /L), with an optimal cut-off level of 0.40kU /L (sensitivity, 66.7% and specificity, 77.8%). The positive decision point of pine nut-sIgE (specificity, 100%) to distinguish the allergic and tolerant groups was 2.84kU /L. However, there was no difference in pine nut-sIgE levels between the anaphylaxis and non-anaphylaxis cases. CONCLUSION: About 30% of children with pine nut allergy experienced anaphylaxis. The optimal cut-off level of pine nut-sIgE to distinguish the allergic and tolerant groups was 0.40kU /L and the positive decision point was 2.84kU /L.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180203
[Lr] Last revision date:180203
[St] Status:Publisher

  2 / 752 MEDLINE  
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[PMID]: 27773603
[Au] Autor:van der Valk JP; Gerth van Wijk R; Baumert JL; Nordlee JA; Vlieg-Boerstra BJ; de Groot H; Dubois AE; de Jong NW
[Ad] Address:Department of Internal Medicine, Allergology, Erasmus MC, Rotterdam, the Netherlands. Electronic address: j.p.m.vandervalk@erasmusmc.nl.
[Ti] Title:Threshold dose distribution and eliciting dose of cashew nut allergy.
[So] Source:Ann Allergy Asthma Immunol;117(6):712-714, 2016 12.
[Is] ISSN:1534-4436
[Cp] Country of publication:United States
[La] Language:eng
[Mh] MeSH terms primary: Anacardium/immunology
Nut Hypersensitivity/immunology
Nuts/immunology
[Mh] MeSH terms secundary: Adolescent
Allergens/immunology
Female
Follow-Up Studies
Humans
Male
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Allergens)
[Em] Entry month:1712
[Cu] Class update date: 171212
[Lr] Last revision date:171212
[Js] Journal subset:IM
[Da] Date of entry for processing:161025
[St] Status:MEDLINE

  3 / 752 MEDLINE  
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[PMID]: 28950267
[Au] Autor:Schocker F; Scharf A; Kull S; Jappe U
[Ad] Address:Division of Clinical and Molecular Allergology, Research Center Borstel, Priority Research Area Asthma and Allergy, Airway Research Center North (ARCN), German Center for Lung Research (DZL), Borstel, Germany.
[Ti] Title:Detection of the Peanut Allergens Ara h 2 and Ara h 6 in Human Breast Milk: Development of 2 Sensitive and Specific Sandwich ELISA Assays.
[So] Source:Int Arch Allergy Immunol;174(1):17-25, 2017.
[Is] ISSN:1423-0097
[Cp] Country of publication:Switzerland
[La] Language:eng
[Ab] Abstract:BACKGROUND: Little is known about breast milk as a vehicle for tolerance development or sensitization to peanuts very early in life. Thus, well-characterized and highly sensitive detection systems for the reliable determination of peanut allergens in breast milk are mandatory. METHODS: For the quantification of the marker allergens Ara h 2 and Ara h 6 in the low nanogram per milliliter range in breast milk samples of a German cohort, sensitive and highly specific sandwich ELISAs were optimized and validated. RESULTS: The Ara h 2 ELISA revealed a limit of detection (LOD) of 1.3 ng Ara h 2/mL and a quantification range of 2.3-250 ng/mL, the Ara h 6 ELISA showed an LOD of 0.7 ng/mL and a working range of 1.1-14.4 ng/mL. The assays showed no relevant cross-reactivity against other potentially cross-reactive legume, seed, and tree nut extracts (<0.01%, except for Ara h 1 in the Ara h 2 ELISA <0.1%). Ara h 2 was detectable in breast milk samples from 14/40 (35%) of the participants in concentrations from 2.3 to 184 ng/mL, Ara h 6 appeared in 9/40 (22.5%) of the lactating mothers between 1.1 and 9.7 ng/mL, and 1 highly positive sample with 79 ng/mL. Both allergens appeared at the same time points, but Ara h 6 in lower concentrations than Ara h 2. CONCLUSIONS: Sensitive and specific diagnostic tools for the determination of Ara h 2 and Ara h 6 in human breast milk were established. The kinetics of secreted Ara h 2 and Ara h 6 seem to be similar but with a difference in concentration. Follow-up investigations on their tolerogenic or sensitizing properties in breast milk become now accessible.
[Mh] MeSH terms primary: 2S Albumins, Plant/analysis
Antigens, Plant/analysis
Enzyme-Linked Immunosorbent Assay/methods
Glycoproteins/analysis
Milk, Human/chemistry
Peanut Hypersensitivity/diagnosis
Plant Proteins/immunology
[Mh] MeSH terms secundary: Allergens/analysis
Arachis/immunology
Cross Reactions/immunology
Female
Humans
Lactation/physiology
Limit of Detection
Peanut Hypersensitivity/prevention & control
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (2S Albumins, Plant); 0 (Allergens); 0 (Antigens, Plant); 0 (Ara h 2 allergen, Arachis hypogaea); 0 (Ara h 6 allergen, Arachis hypogaea); 0 (Glycoproteins); 0 (Plant Proteins)
[Em] Entry month:1710
[Cu] Class update date: 171031
[Lr] Last revision date:171031
[Js] Journal subset:IM
[Da] Date of entry for processing:170927
[St] Status:MEDLINE
[do] DOI:10.1159/000479388

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[PMID]: 28595954
[Au] Autor:Javed B; Padfield P; Sperrin M; Simpson A; Mills ENC
[Ad] Address:Manchester Institute of Biotechnology, Manchester M17DN, UK; Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Manchester M17DN, UK.
[Ti] Title:A protocol for a systematic review to identify allergenic tree nuts and the molecules responsible for their allergenic properties.
[So] Source:Food Chem Toxicol;106(Pt A):411-416, 2017 Aug.
[Is] ISSN:1873-6351
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Food regulations require that tree nuts and derived ingredients are included on food labels in order to help individuals with IgE-mediated allergies to avoid them. However, there is no consensus regarding which tree nut species should be included in this definition and specified on food labels. Allergen detection methods used for monitoring foods target allergen molecules, but it not clear which are the most relevant molecules to choose. A modified population-exposure-comparators-outcome (PECO) approach has been developed to systematically review the evidence regarding (1) which allergenic tree nuts should be included in food allergen labelling lists and (2) which are the clinically relevant allergens which should be used as analytical targets. A search strategy and criteria against which the evidence will be evaluated have been developed. The resulting evidence will be used to rank tree nuts with regards their ability to cause IgE-mediated allergies, and allergen molecules regarding their capacity to elicit an allergic reaction. The results of the systematic review will enable risk assessors and managers to identify tree nut species that should be included in food allergen labelling lists and ensure analytical methods for determination of allergens in foods are targeting appropriate molecules.
[Mh] MeSH terms primary: Nut Hypersensitivity/diagnosis
Nuts/immunology
[Mh] MeSH terms secundary: Allergens/immunology
Clinical Protocols
Humans
Immunoglobulin E/immunology
Nut Hypersensitivity/etiology
Nut Hypersensitivity/immunology
Nuts/adverse effects
Nuts/chemistry
Pilot Projects
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Allergens); 37341-29-0 (Immunoglobulin E)
[Em] Entry month:1709
[Cu] Class update date: 170927
[Lr] Last revision date:170927
[Js] Journal subset:IM
[Da] Date of entry for processing:170610
[St] Status:MEDLINE

  5 / 752 MEDLINE  
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[PMID]: 28359600
[Au] Autor:Couch C; Franxman T; Greenhawt M
[Ad] Address:Division of Allergy and Clinical Immunology, The University of Michigan Medical School, Ann Arbor, Michigan; Allergy Asthma Clinic, Ltd, Phoenix, Arizona.
[Ti] Title:Characteristics of tree nut challenges in tree nut allergic and tree nut sensitized individuals.
[So] Source:Ann Allergy Asthma Immunol;118(5):591-596.e3, 2017 May.
[Is] ISSN:1534-4436
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Characteristics and outcomes of tree nut (TN) oral food challenges (OFCs) in patients with TN allergy or sensitization alone are poorly studied. OBJECTIVE: To determine the relation between TN sensitization levels and OFC outcomes. METHODS: Open TN OFCs performed from 2007 through 2015 at a referral center were analyzed to compare outcome based on skin prick test (SPT) wheal size, food-specific immunoglobulin E (sIgE), peanut co-allergy, and TN sensitization only vs TN allergy with sensitization to other TNs. Delayed OFC was defined as longer than 12 months from the time of an sIgE level lower than 2 kUA/L. RESULTS: Overall passage rate was 86% for 156 TN OFCs in 109 patients (54 almond, 28 cashew, 27 walnut, 18 hazelnut, 14 pecan, 13 pistachio, and 2 Brazil nut). Passage rates were 76% (n = 67) in patients with a history of TN allergy who were challenged to another TN to which they were sensitized and 91% (n = 65) in those with TN sensitization only (mean sIgE 1.53 kUA/L; range 0.35-9.14). Passage rates were 89% (n = 110 of 124) for a TN sIgE level lower than 2 kUA/L and 69% (11 of 16) for a TN sIgE level of at least 2 kUA/L. In 44 challenges in patients with peanut allergy and TN co-sensitization, the TN OFC passage rate was 96%. In 41 TN OFCs with a TN SPT wheal size of at least 3 mm, 61% passed, with a mean wheal size of 4.8 mm (range 3-11) in those passing vs 9 mm (range 3-20) in those failing. CONCLUSION: TN challenges are frequently passed in patients with TN sensitization with or without a history of TN reactivity despite a TN SPT wheal of at least 3 mm or a TN sIgE level of at least 2 kUA/L. Nearly all patients with peanut allergy and TN co-sensitization passed the TN challenge, questioning the clinical relevance of "co-allergy."
[Mh] MeSH terms primary: Allergens/immunology
Nut Hypersensitivity/diagnosis
Nut Hypersensitivity/immunology
Nuts/adverse effects
[Mh] MeSH terms secundary: Allergens/classification
Antibody Specificity/immunology
Child
Child, Preschool
Comorbidity
Female
Humans
Immunization
Immunoglobulin E/blood
Immunoglobulin E/immunology
Male
Phenotype
Retrospective Studies
Skin Tests
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Allergens); 37341-29-0 (Immunoglobulin E)
[Em] Entry month:1708
[Cu] Class update date: 170804
[Lr] Last revision date:170804
[Js] Journal subset:IM
[Da] Date of entry for processing:170401
[St] Status:MEDLINE

  6 / 752 MEDLINE  
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[PMID]: 28211344
[Au] Autor:González-Mancebo E; Alonso Díaz de Durana MD; García Estringana Y; Meléndez Baltanás A; Rodriguez-Alvarez M; de la Hoz Caballer B; Del Prado N; Fernández-Rivas M
[Ad] Address:Allergy Department, Hospital Universitario de Fuenlabrada, Madrid, Spain.
[Ti] Title:Validation of Recipes for Double-Blind Placebo-Controlled Challenges With Milk, Egg White, and Hazelnut.
[So] Source:J Investig Allergol Clin Immunol;27(1):40-45, 2017.
[Is] ISSN:1018-9068
[Cp] Country of publication:Spain
[La] Language:eng
[Ab] Abstract:BACKGROUND: The double-blind, placebo-controlled food challenge (DBPCFC) is considered the definitive diagnostic test for food allergy. Nevertheless, validated recipes for masking the foods are scarce, have not been standardized, and differ between centers. Sensory evaluation techniques such as the triangle test are necessary to validate the recipes used for DBPCFC. METHODS: We developed 3 recipes for use in DBPCFC with milk, egg white, and hazelnut and used the triangle test to validate them in a 2-phase study in which 197 volunteers participated. In each phase, participants tried 3 samples (2 active-1 placebo or 2 placebo-1 active) and had to identify the odd one. In phase 1, the 3 samples were given simultaneously, whereas in phase 2, the 3 samples of foods that failed validation in phase 1 were given sequentially. A visual analog scale (VAS) ranging from 1 to 10 was used to evaluate how much participants liked the recipes. RESULTS: In phase 1, the egg white recipe was validated (n=89 volunteers, 38.9% found the odd sample, P=.16). Milk and hazelnut recipes were validated in phase 2 (for both foods, n=30 participants, 36.7% found the odd sample, P=.36). Median VAS scores for the 3 recipes ranged from 6.6 to 9.7. CONCLUSIONS: We used sensory testing to validate milk, egg white, and hazelnut recipes for use in DBPCFC. The validated recipes are easy to prepare in a clinical setting, provide the equivalent of 1 serving dose, and were liked by most participants.
[Mh] MeSH terms primary: Corylus
Egg Hypersensitivity/diagnosis
Egg Proteins/administration & dosage
Immunologic Tests
Milk Hypersensitivity/diagnosis
Milk Proteins/administration & dosage
Nut Hypersensitivity/diagnosis
Plant Preparations/administration & dosage
[Mh] MeSH terms secundary: Adult
Cooking
Corylus/adverse effects
Corylus/immunology
Double-Blind Method
Egg Hypersensitivity/immunology
Egg Proteins/adverse effects
Egg Proteins/immunology
Female
Humans
Male
Middle Aged
Milk Hypersensitivity/immunology
Milk Proteins/adverse effects
Milk Proteins/immunology
Nut Hypersensitivity/immunology
Patient Satisfaction
Plant Preparations/adverse effects
Plant Preparations/immunology
Predictive Value of Tests
Reproducibility of Results
Sensation
Spain
[Pt] Publication type:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; VALIDATION STUDIES
[Nm] Name of substance:0 (Egg Proteins); 0 (Milk Proteins); 0 (Plant Preparations)
[Em] Entry month:1706
[Cu] Class update date: 170606
[Lr] Last revision date:170606
[Js] Journal subset:IM
[Da] Date of entry for processing:170218
[St] Status:MEDLINE
[do] DOI:10.18176/jiaci.0084

  7 / 752 MEDLINE  
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[PMID]: 28110058
[Au] Autor:Blankestijn MA; Remington BC; Houben GF; Baumert JL; Knulst AC; Blom WM; Klemans RJ; Taylor SL
[Ad] Address:Department of Dermatology/Allergology, University Medical Center Utrecht, Utrecht, The Netherlands. Electronic address: m.a.blankestijn@umcutrecht.nl.
[Ti] Title:Threshold Dose Distribution in Walnut Allergy.
[So] Source:J Allergy Clin Immunol Pract;5(2):376-380, 2017 Mar - Apr.
[Is] ISSN:2213-2201
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: In food allergy, eliciting doses (EDs) of foods on a population level can improve risk management and labeling strategies for the food industry and regulatory authorities. Previously, data available for walnut were unsuitable to determine EDs. OBJECTIVE: The objective of this study was to determine EDs for walnut allergic adults and to compare with previously established threshold data for peanut and tree nuts. METHODS: Prospectively, adult subjects with a suspected walnut allergy underwent a low-dose double-blind, placebo-controlled food challenge. Individual no observed and lowest observed adverse effect levels were determined and log-normal, log-logistic, and Weibull models were fit to the data. Estimated ED values were calculated for the ED ED , and ED , the dose respectively predicted to provoke an allergic reaction in 5%, 10%, and 50% of the walnut allergic population. RESULTS: Fifty-seven subjects were challenged and 33 subjects were confirmed to be walnut allergic. Objective symptoms occurred in 20 of the positive challenges (61%). The cumulative EDs in the distribution models ranged from 3.1 to 4.1 mg for the ED , from 10.6 to 14.6 mg walnut protein for the ED , and from 590 to 625 mg of walnut protein for the ED . CONCLUSIONS: Our data indicate that population EDs for walnut are slightly higher compared with those for peanut and hazelnut allergy. Currently available data indicate that the ED values for hazelnut could be used as a conservative temporary placeholder when implementing risk management strategies for other tree nuts where little or no food challenge data are available.
[Mh] MeSH terms primary: Allergens/immunology
Juglans/immunology
Nut Hypersensitivity/diet therapy
Nut Hypersensitivity/diagnosis
[Mh] MeSH terms secundary: Administration, Oral
Adult
Female
Food Labeling
Humans
Immunization
Male
Prospective Studies
Risk
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Allergens)
[Em] Entry month:1711
[Cu] Class update date: 171107
[Lr] Last revision date:171107
[Js] Journal subset:IM
[Da] Date of entry for processing:170123
[St] Status:MEDLINE

  8 / 752 MEDLINE  
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[PMID]: 28070926
[Au] Autor:Wangorsch A; Jamin A; Lidholm J; Gräni N; Lang C; Ballmer-Weber B; Vieths S; Scheurer S
[Ad] Address:VPr1 Research Group Molecular Allergology, Paul-Ehrlich-Institut, Langen, Germany.
[Ti] Title:Identification and implication of an allergenic PR-10 protein from walnut in birch pollen associated walnut allergy.
[So] Source:Mol Nutr Food Res;61(4), 2017 Apr.
[Is] ISSN:1613-4133
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:SCOPE: English walnut (Juglans regia) belongs to the most important allergenic tree nuts. Co-sensitization with birch (Betula verrucosa) pollen has been reported. We aimed to identify a walnut allergen homologous to the major birch pollen allergen Bet v 1. METHODS AND RESULTS: A cDNA encoding a Bet v 1-homologous allergen (Jug r 5) in walnut kernels was cloned by RT-PCR. Jug r 5 was expressed in Escherichia coli, purified by column chromatography and characterized by circular dichroism spectroscopy. Specific IgE levels to walnut, Bet v 1, and Jug r 5 in birch pollen allergics (n = 16) with concomitant walnut allergy were measured by ImmunoCAP: 44% of the patients were tested positive to walnut while 94% were reactive to Jug r 5, and 100% to Bet v 1. Jug r 5 and Bet v 1 allergens showed bidirectional IgE cross-reactivity by competitive ELISA and were capable of inducing histamine release from effector cells. Immunoblot competition experiments demonstrated the presence of IgE-reactive Jug r 5 in walnut extract, but at low levels. CONCLUSION: A Bet v 1-like allergen was identified in walnut. Diagnostic use of Jug r 5 will compensate for the low sensitivity of walnut extract for patients with birch pollen associated walnut allergy.
[Mh] MeSH terms primary: Allergens/immunology
Antigens, Plant/pharmacology
Betula/chemistry
Hypersensitivity
Juglans/chemistry
Plant Proteins/metabolism
Pollen/immunology
[Mh] MeSH terms secundary: Amino Acid Sequence
Antigens, Plant/metabolism
Cross Reactions
Female
Histamine Release
Humans
Immunoblotting
Immunoglobulin E/immunology
Nuts/immunology
Plant Proteins/immunology
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Allergens); 0 (Antigens, Plant); 0 (PR-10 protein, walnut birch pollen); 0 (Plant Proteins); 126161-14-6 (Bet v 1 allergen, Betula); 37341-29-0 (Immunoglobulin E)
[Em] Entry month:1711
[Cu] Class update date: 171113
[Lr] Last revision date:171113
[Js] Journal subset:IM
[Da] Date of entry for processing:170111
[St] Status:MEDLINE
[do] DOI:10.1002/mnfr.201600902

  9 / 752 MEDLINE  
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[PMID]: 27966914
[Au] Autor:Mattison CP; Grimm CC; Li Y; Chial HJ; McCaslin DR; Chung SY; Bren-Mattison Y; Wasserman RL
[Ad] Address:Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture , 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
[Ti] Title:Identification and Characterization of Ana o 3 Modifications on Arginine-111 Residue in Heated Cashew Nuts.
[So] Source:J Agric Food Chem;65(2):411-420, 2017 Jan 18.
[Is] ISSN:1520-5118
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Raw and roasted cashew nut extracts were evaluated for protein modifications by mass spectrometry. Independent modifications on the Arg-111 residue of Ana o 3 were observed in roasted but not raw cashew nuts. The mass changes of 72.0064 or 53.9529 Da are consistent with the formation of carboxyethyl and hydroimidazolone modifications at the Arg-111 residue. These same modifications were observed in Ana o 3 purified from roasted but not raw cashew nuts, albeit at a relatively low occurrence. Circular dichroism indicated that Ana o 3 purified from raw and roasted cashew nuts had similar secondary structure, and dynamic light scattering analysis indicated there was no observable difference in particle size. The stability of Ana o 3 purified from raw and roasted cashew nuts to trypsin was similar in the absence of or following treatment with a reducing agent. Only minor differences in IgE binding to Ana o 3 were observed by ELISA among a cohort of cashew-allergic patient sera.
[Mh] MeSH terms primary: Anacardium/chemistry
Antigens, Plant/chemistry
Arginine/chemistry
Food Handling/methods
Plant Proteins/chemistry
[Mh] MeSH terms secundary: Antigens, Plant/isolation & purification
Antigens, Plant/metabolism
Circular Dichroism
Dynamic Light Scattering
Enzyme-Linked Immunosorbent Assay
Heating
Humans
Imidazoles/chemistry
Immune Sera
Immunoglobulin E/metabolism
Nut Hypersensitivity/immunology
Nuts/chemistry
Particle Size
Plant Proteins/isolation & purification
Plant Proteins/metabolism
Protein Conformation
Trypsin/chemistry
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Ana o 3 protein, Anacardium occidentale); 0 (Antigens, Plant); 0 (Imidazoles); 0 (Immune Sera); 0 (Plant Proteins); 0 (imidazolone); 37341-29-0 (Immunoglobulin E); 94ZLA3W45F (Arginine); EC 3.4.21.4 (Trypsin)
[Em] Entry month:1705
[Cu] Class update date: 170502
[Lr] Last revision date:170502
[Js] Journal subset:IM
[Da] Date of entry for processing:161215
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b04134

  10 / 752 MEDLINE  
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[PMID]: 27936684
[Au] Autor:Zhang YZ; Du WX; Fan Y; Yi J; Lyu SC; Nadeau KC; Thomas AL; McHugh T
[Ad] Address:Western Regional Research Center, Pacific West Area, Agricultural Research Service, U.S. Department of Agriculture , 800 Buchanan Street, Albany, California 94710, United States.
[Ti] Title:Purification and Characterization of a Black Walnut (Juglans nigra) Allergen, Jug n 4.
[So] Source:J Agric Food Chem;65(2):454-462, 2017 Jan 18.
[Is] ISSN:1520-5118
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Tree nuts as a group cause a significant number of fatal anaphylactic reactions to foods. Walnuts (Juglans spp.) are one of the leading causes of allergic reactions to tree nuts in the U.S. and Japan. The purpose of this study was to purify and characterize potential food allergens from black walnut. Here, we report the isolation of the black walnuts allergen Jug n 4 (an 11S globulin) by ammonium sulfate precipitation, hydrophobic interaction, and size exclusion chromatography. Reducing SDS-PAGE analysis indicated that purified Jug n 4 consists of three major bands. N-Terminal sequencing data of these bands indicated that they were the results of a post-transcriptional protease cleavage of the mature protein at a site that consists of a known conserved protease recognition motif, NGXEET. Western blot experiments revealed that 32% of the sera from 25 patients with double-blind, placebo-controlled clinical walnut allergy contained IgE antibodies that recognized Jug n 4, indicating that it is a walnut allergen. Identifying this and additional allergens may facilitate the understanding of the allergenicity of seed storage proteins in tree nuts and their cross-reactivity.
[Mh] MeSH terms primary: Allergens/isolation & purification
Antigens, Plant/isolation & purification
Juglans/chemistry
Nut Hypersensitivity/immunology
Plant Proteins/isolation & purification
[Mh] MeSH terms secundary: Adolescent
Allergens/genetics
Allergens/immunology
Amino Acid Motifs
Antigens, Plant/genetics
Antigens, Plant/immunology
Chemical Precipitation
Child
Chromatography, Gel
Circular Dichroism
Electrophoresis, Polyacrylamide Gel
Humans
Immune Sera
Juglans/immunology
Plant Proteins/genetics
Plant Proteins/immunology
RNA Processing, Post-Transcriptional
Young Adult
[Pt] Publication type:CONTROLLED CLINICAL TRIAL; JOURNAL ARTICLE
[Nm] Name of substance:0 (Allergens); 0 (Antigens, Plant); 0 (Immune Sera); 0 (Jug n 4 allergen, Juglans nigra); 0 (Plant Proteins); 0 (legumin protein, plant)
[Em] Entry month:1705
[Cu] Class update date: 170502
[Lr] Last revision date:170502
[Js] Journal subset:IM
[Da] Date of entry for processing:161213
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b04387


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