Database : MEDLINE
Search on : Respirovirus and Infections [Words]
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[PMID]: 28742120
[Au] Autor:Skappak C; Ilarraza R; Wu YQ; Drake MG; Adamko DJ
[Ad] Address:Division of Emergency Medicine, McMaster University, Hamilton, Ontario, Canada.
[Ti] Title:Virus-induced asthma attack: The importance of allergic inflammation in response to viral antigen in an animal model of asthma.
[So] Source:PLoS One;12(7):e0181425, 2017.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Asthma exacerbation can be a life-threatening condition, and is most often triggered by common respiratory viruses. Poor asthma control and worsening of respiratory function is associated with increased airway inflammation, including eosinophilia. Prevention of asthma exacerbation relies on treatment with corticosteroids, which preferentially inhibit allergic inflammation like eosinophils. Human studies demonstrate that inactivated virus can trigger eosinophil activation in vitro through antigen presentation and memory CD4+ lymphocytes. We hypothesized that animals with immunologic memory to a respiratory virus would also develop airway hyperresponsiveness in response to a UV-inactivated form of the virus if they have pre-existing allergic airway inflammation. Guinea pigs were ovalbumin-sensitized, infected with live parainfluenza virus (PIV), aerosol-challenged with ovalbumin, and then re-inoculated 60 days later with live or UV-inactivated PIV. Some animals were either treated with dexamethasone prior to the second viral exposure. Lymphocytes were isolated from parabronchial lymph nodes to confirm immunologic memory to the virus. Airway reactivity was measured and inflammation was assessed using bronchoalveolar lavage and lung histology. The induction of viral immunologic memory was confirmed in infected animals. Allergen sensitized and challenged animals developed airway hyperreactivity with eosinophilic airway inflammation when re-exposed to UV-inactivated PIV, while non-sensitized animals did not. Airway hyperreactivity in the sensitized animals was inhibited by pre-treatment with dexamethasone. We suggest that the response of allergic inflammation to virus antigen is a significant factor causing asthma exacerbation. We propose that this is one mechanism explaining how corticosteroids prevent virus-induced asthma attack.
[Mh] MeSH terms primary: Asthma/virology
Parainfluenza Virus 1, Human/immunology
Respiratory Hypersensitivity/virology
Respirovirus Infections/complications
[Mh] MeSH terms secundary: Animals
Anti-Inflammatory Agents/therapeutic use
Asthma/drug therapy
Asthma/immunology
Dexamethasone/therapeutic use
Disease Models, Animal
Female
Guinea Pigs
Humans
Immunologic Memory/drug effects
Inflammation/drug therapy
Inflammation/immunology
Inflammation/virology
Lymphocytes/immunology
Lymphocytes/virology
Respiratory Hypersensitivity/drug therapy
Respiratory Hypersensitivity/immunology
Respirovirus Infections/drug therapy
Respirovirus Infections/immunology
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Anti-Inflammatory Agents); 7S5I7G3JQL (Dexamethasone)
[Em] Entry month:1709
[Cu] Class update date: 180207
[Lr] Last revision date:180207
[Js] Journal subset:IM
[Da] Date of entry for processing:170726
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181425

  2 / 2909 MEDLINE  
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[PMID]: 28463659
[Au] Autor:Kosutic-Gulija T; Slovic A; Ljubin-Sternak S; Mlinaric-Galinovic G; Forcic D
[Ad] Address:1​Centre for Research and Knowledge Transfer in Biotechnology, University of Zagreb, Zagreb, Croatia.
[Ti] Title:Genetic analysis of human parainfluenza virus type 3 obtained in Croatia, 2011-2015.
[So] Source:J Med Microbiol;66(4):502-510, 2017 Apr.
[Is] ISSN:1473-5644
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:PURPOSE: This study investigated the HPIV3 circulating strains in Croatia and whether the other parts of HPIV3 genome (F gene and HN 582 nucleotides fragment) could be equally suitable for genetic and phylogenetic analysis. METHODOLOGY: Clinical materials were collected in period 2011-2015 from children suffering from respiratory illnesses. In positive HPIV3 samples viral genome was partially amplified and sequenced for HN and F genes. Obtained sequences were analysed by phylogenetic analysis and genetic characterization was performed. RESULTS: All samples from this study belonged to subcluster C and over a short period of time, genetic lineage C3a gained prevalence over the other C genetic lineages, from 39 % in 2011 to more than 90 % in 2013 and 2014. Phylogenetic classifications of HPIV3 based on the entire HN gene, HN 582 nt fragment and entire fusion (F) gene showed identical classification results for Croatian strains and the reference strains. Molecular analysis of the F and HN glycoproteins, showed their similar nucleotide diversity (Fcds P=0.0244 and HNcds P=0.0231) and similar Ka/Ks ratios (F Ka/Ks=0.0553 and HN Ka/Ks=0.0428). Potential N-glycosylation sites, cysteine residues and antigenic sites are generally strongly conserved in HPIV3 glycoproteins from both our and the reference samples. CONCLUSION: The HPIV3 subclaster C3 (genetic lineage C3a) became the most detected circulating HPIV3 strain in Croatia. The results indicated that the HN 582 nt and the entire F gene sequences were as good for phylogenetic analysis as the entire HN gene sequence.
[Mh] MeSH terms primary: Genome, Viral/genetics
HN Protein/genetics
Parainfluenza Virus 3, Human/genetics
Respirovirus Infections/epidemiology
Viral Fusion Proteins/genetics
[Mh] MeSH terms secundary: Base Sequence
Child, Preschool
Croatia/epidemiology
Humans
Infant
Parainfluenza Virus 3, Human/classification
Parainfluenza Virus 3, Human/isolation & purification
Phylogeny
Respirovirus Infections/virology
Sequence Analysis, RNA
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (HN Protein); 0 (Viral Fusion Proteins)
[Em] Entry month:1705
[Cu] Class update date: 180116
[Lr] Last revision date:180116
[Js] Journal subset:IM
[Da] Date of entry for processing:170503
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000459

  3 / 2909 MEDLINE  
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[PMID]: 29093083
[Au] Autor:Mostafa HH; Vogel P; Srinivasan A; Russell CJ
[Ad] Address:Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105, USA.
[Ti] Title:Dynamics of Sendai virus spread, clearance, and immunotherapeutic efficacy after hematopoietic cell transplant imaged non-invasively in mice.
[So] Source:J Virol;, 2017 Nov 01.
[Is] ISSN:1098-5514
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:There are no approved vaccines or virus-specific treatments for human parainfluenza viruses (PIVs), which have recently been reclassified into species and and and These viruses cause morbidity and mortality in immunocompromised patients including those undergoing hematopoietic cell transplant (HCT). No small-animal models exist for non-invasive imaging of respiratory viral infection in the HCT host despite the utility such a system would offer to monitor prolonged infection, its clearance, and treatment options. We used a luciferase-expressing reporter virus to non-invasively image in mice the infection of (strain: Sendai virus, SeV), the murine counterpart of HPIV1. Independent of disease severity, clearance of infection began approximately 21 days after HCT, largely due to recovery of CD8+ T cells. Immunotherapy with granulocyte colony-stimulating factor (G-CSF) and adoptive transfer of natural killer (NK) cells provided limited therapeutic benefit. Treatment with a fusion (F) protein-specific monoclonal antibody arrested the spread of lung infection and reduced disease severity even when treatment was delayed up to 10 days post infection but had little observable effect on upper respiratory tract infection. Adoptive transfer of virus-specific T cells 10 days post infection accelerated clearance by 5 days, reduced the extent of infection throughout the respiratory tract, and reduced disease severity. Overall, the results support investigation of clinical treatment of respiratory virus infection in the HCT host with monoclonal antibodies and adoptive T-cell transfer; the imaging system should be extendable to other respiratory viruses such as RSV and influenza virus. Parainfluenza viruses are a major cause of disease and death due to respiratory virus infection in the immunocompromised host including those undergoing Bone Marrow Transplantation. There are currently no effective treatment measures. We non-invasively imaged mice undergoing bone marrow transplant and infected with Sendai virus, a murine parainfluenza virus (respirovirus). For the first time, we show the therapeutic windows of adoptive T-cell therapy and monoclonal antibody to the fusion (F) protein in clearing Sendai virus from the respiratory tract and reducing disease severity. Mice tolerated these treatments without any detectable toxicity. This paves the way for studies assessing the safety of T-cell therapy against parainfluenza virus in humans. Adoptive T-cell therapy in humans against other blood-borne viruses has been shown to be safe and effective. Our model of non-invasive imaging in transplanted mice may be well suited to track other respiratory virus infections and develop novel preventive and therapeutic strategies.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1711
[Cu] Class update date: 171102
[Lr] Last revision date:171102
[St] Status:Publisher

  4 / 2909 MEDLINE  
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[PMID]: 28963878
[Au] Autor:Thomazelli LM; Oliveira DBL; Durigon GS; Whitaker B; Kamili S; Berezin EN; Durigon EL
[Ad] Address:Universidade de São Paulo (USP), Instituto de Ciências Biomédicas, São Paulo, SP, Brazil. Electronic address: lucmt@usp.br.
[Ti] Title:Human parainfluenza virus surveillance in pediatric patients with lower respiratory tract infections: a special view of parainfluenza type 4.
[So] Source:J Pediatr (Rio J);, 2017 Sep 28.
[Is] ISSN:1678-4782
[Cp] Country of publication:Brazil
[La] Language:eng
[Ab] Abstract:OBJECTIVE: Characterize the role of human parainfluenza virus and its clinical features in Brazilian children under 2 years of age presenting with acute lower respiratory tract infections. METHODS: Real-time assays were used to identify strains of human parainfluenza virus and other common respiratory viruses in nasopharyngeal aspirates. One thousand and two children presenting with acute lower respiratory tract illnesses were enrolled from February 2008 to August 2010. RESULTS: One hundred and four (10.4%) patients were human parainfluenza virus positive, of whom 60 (57.7%) were positive for human parainfluenza virus-3, 30 (28.8%) for human parainfluenza virus-4, 12 (11.5%) for human parainfluenza virus-1, and two (1.9%) for human parainfluenza virus-2. Seven (6.7%) patients had more than one strain of human parainfluenza virus detected. The most frequent symptoms were tachypnea and cough, similar to other viral respiratory infections. Clinical manifestations did not differ significantly between human parainfluenza virus-1, -2, -3, and -4 infections. Human parainfluenza virus-1, -3, and -4 were present in the population studied throughout the three years of surveillance, with human parainfluenza virus-3 being the predominant type identified in the first two years. CONCLUSION: Human parainfluenza viruses contribute substantially to pediatric acute respiratory illness (ARI) in Brazil, with nearly 30% of this contribution attributable to human parainfluenza virus-4.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1710
[Cu] Class update date: 171016
[Lr] Last revision date:171016
[St] Status:Publisher

  5 / 2909 MEDLINE  
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[PMID]: 28934360
[Au] Autor:Yan BR; Zhou L; Hu MM; Li M; Lin H; Yang Y; Wang YY; Shu HB
[Ad] Address:College of Life Sciences, Wuhan University, Wuhan, China.
[Ti] Title:PKACs attenuate innate antiviral response by phosphorylating VISA and priming it for MARCH5-mediated degradation.
[So] Source:PLoS Pathog;13(9):e1006648, 2017 Sep.
[Is] ISSN:1553-7374
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Sensing of viral RNA by RIG-I-like receptors initiates innate antiviral response, which is mediated by the central adaptor VISA. How the RIG-I-VISA-mediated antiviral response is terminated at the late phase of infection is enigmatic. Here we identified the protein kinase A catalytic (PKAC) subunits α and ß as negative regulators of RNA virus-triggered signaling in a redundant manner. Viral infection up-regulated cellular cAMP levels and activated PKACs, which then phosphorylated VISA at T54. This phosphorylation abrogated virus-induced aggregation of VISA and primed it for K48-linked polyubiquitination and degradation by the E3 ligase MARCH5, leading to attenuation of virus-triggered induction of downstream antiviral genes. PKACs-deficiency or inactivation by the inhibitor H89 potentiated innate immunity to RNA viruses in cells and mice. Our findings reveal a critical mechanism of attenuating innate immune response to avoid host damage at the late phase of viral infection by the house-keeping PKA kinase.
[Mh] MeSH terms primary: Adaptor Proteins, Signal Transducing/immunology
Cyclic AMP-Dependent Protein Kinase Catalytic Subunits/immunology
Immunity, Innate/immunology
Membrane Proteins/immunology
Respirovirus Infections/immunology
Ubiquitin-Protein Ligases/immunology
[Mh] MeSH terms secundary: Animals
HEK293 Cells
Humans
Immunoblotting
Immunoprecipitation
Mice
Phosphorylation
Sendai virus
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Adaptor Proteins, Signal Transducing); 0 (Membrane Proteins); 0 (VISA protein, human); EC 2.3.2.27 (MARCH5 protein, human); EC 2.3.2.27 (Ubiquitin-Protein Ligases); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinase Catalytic Subunits)
[Em] Entry month:1710
[Cu] Class update date: 171018
[Lr] Last revision date:171018
[Js] Journal subset:IM
[Da] Date of entry for processing:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.ppat.1006648

  6 / 2909 MEDLINE  
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[PMID]: 28704440
[Au] Autor:Horton KC; Dueger EL; Kandeel A; Abdallat M; El-Kholy A; Al-Awaidy S; Kohlani AH; Amer H; El-Khal AL; Said M; House B; Pimentel G; Talaat M
[Ad] Address:Global Disease Detection Center, U.S. Centers for Disease Control and Prevention, Cairo, Egypt.
[Ti] Title:Viral etiology, seasonality and severity of hospitalized patients with severe acute respiratory infections in the Eastern Mediterranean Region, 2007-2014.
[So] Source:PLoS One;12(7):e0180954, 2017.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:INTRODUCTION: Little is known about the role of viral respiratory pathogens in the etiology, seasonality or severity of severe acute respiratory infections (SARI) in the Eastern Mediterranean Region. METHODS: Sentinel surveillance for SARI was conducted from December 2007 through February 2014 at 20 hospitals in Egypt, Jordan, Oman, Qatar and Yemen. Nasopharyngeal and oropharyngeal swabs were collected from hospitalized patients meeting SARI case definitions and were analyzed for infection with influenza, respiratory syncytial virus (RSV), adenovirus (AdV), human metapneumovirus (hMPV) and human parainfluenza virus types 1-3 (hPIV1-3). We analyzed surveillance data to calculate positivity rates for viral respiratory pathogens, describe the seasonality of those pathogens and determine which pathogens were responsible for more severe outcomes requiring ventilation and/or intensive care and/or resulting in death. RESULTS: At least one viral respiratory pathogen was detected in 8,753/28,508 (30.7%) samples tested for at least one pathogen and 3,497/9,315 (37.5%) of samples tested for all pathogens-influenza in 3,345/28,438 (11.8%), RSV in 3,942/24,503 (16.1%), AdV in 923/9,402 (9.8%), hMPV in 617/9,384 (6.6%), hPIV1 in 159/9,402 (1.7%), hPIV2 in 85/9,402 (0.9%) and hPIV3 in 365/9,402 (3.9%). Multiple pathogens were identified in 501/9,316 (5.4%) participants tested for all pathogens. Monthly variation, indicating seasonal differences in levels of infection, was observed for all pathogens. Participants with hMPV infections and participants less than five years of age were significantly less likely than participants not infected with hMPV and those older than five years of age, respectively, to experience a severe outcome, while participants with a pre-existing chronic disease were at increased risk of a severe outcome, compared to those with no reported pre-existing chronic disease. CONCLUSIONS: Viral respiratory pathogens are common among SARI patients in the Eastern Mediterranean Region. Ongoing surveillance is important to monitor changes in the etiology, seasonality and severity of pathogens of interest.
[Mh] MeSH terms primary: Respiratory Tract Infections/classification
Respiratory Tract Infections/virology
[Mh] MeSH terms secundary: Adenoviridae/classification
Adenoviridae/isolation & purification
Child
Child, Preschool
Female
Humans
Influenza A virus/classification
Influenza A virus/isolation & purification
Inpatients
Male
Mediterranean Region/epidemiology
Metapneumovirus/classification
Metapneumovirus/isolation & purification
Population Surveillance
Respiratory Syncytial Virus, Human/classification
Respiratory Syncytial Virus, Human/isolation & purification
Respiratory Tract Infections/epidemiology
Respirovirus/classification
Respirovirus/isolation & purification
Seasons
Severity of Illness Index
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1709
[Cu] Class update date: 170922
[Lr] Last revision date:170922
[Js] Journal subset:IM
[Da] Date of entry for processing:170714
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180954

  7 / 2909 MEDLINE  
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[PMID]: 28472480
[Au] Autor:Aguayo-Hiraldo PI; Arasaratnam RJ; Tzannou I; Kuvalekar M; Lulla P; Naik S; Martinez CA; Piedra PA; Vera JF; Leen AM
[Ad] Address:Center for Cell and Gene Therapy, Baylor College of Medicine, Texas Children's Hospital, and Houston Methodist Hospital.
[Ti] Title:Characterizing the Cellular Immune Response to Parainfluenza Virus 3.
[So] Source:J Infect Dis;216(2):153-161, 2017 Jul 15.
[Is] ISSN:1537-6613
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Parainfluenza virus type 3 (PIV3) infections are a major cause of morbidity and mortality in immunocompromised individuals, with no approved therapies. Our group has demonstrated the safety and efficacy of adoptively transferred virus-specific T cells for the prevention and treatment of a broad range of viral infections including BK virus, cytomegalovirus, adenovirus, human herpesvirus 6, and Epstein-Barr virus. However, this approach is restricted to well-characterized viruses with known immunogenic/protective T-cell target antigens, precluding extension to PIV3. We now characterize the cellular immune response to all 7 PIV3-encoded antigens in 17 healthy donors and define a hierarchy of immunogenicity based on the frequency of responding donors and the magnitude of specific cells. We show that reactive populations of both CD4+ and CD8+ T cells are capable of producing Th1-polarized effector cytokines and killing PIV3-expressing targets. Furthermore, we confirm the clinical relevance of these cells by demonstrating a direct correlation between the presence of PIV3-specific T cells and viral control in allogeneic hematopoietic stem cell transplant recipients. Taken together, our findings support the clinical use of PIV3-specific T cells produced with our Good Manufacturing Practice-compliant manufacturing process, in immunocompromised patients with uncontrolled infections.
[Mh] MeSH terms primary: Antigens, Viral/immunology
Immunity, Cellular
Leukocytes, Mononuclear/virology
Parainfluenza Virus 3, Human
Respirovirus Infections/immunology
T-Lymphocytes/immunology
[Mh] MeSH terms secundary: Child, Preschool
Cytokines/immunology
Female
Humans
Immunotherapy
Infant
Male
Middle Aged
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antigens, Viral); 0 (Cytokines)
[Em] Entry month:1709
[Cu] Class update date: 170907
[Lr] Last revision date:170907
[Js] Journal subset:AIM; IM
[Da] Date of entry for processing:170505
[St] Status:MEDLINE
[do] DOI:10.1093/infdis/jix203

  8 / 2909 MEDLINE  
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[PMID]: 28276287
[Au] Autor:Cichero E; Tonelli M; Novelli F; Tasso B; Delogu I; Loddo R; Bruno O; Fossa P
[Ad] Address:a Department of Pharmacy , University of Genoa , Genoa , Italy.
[Ti] Title:Benzimidazole-based derivatives as privileged scaffold developed for the treatment of the RSV infection: a computational study exploring the potency and cytotoxicity profiles.
[So] Source:J Enzyme Inhib Med Chem;32(1):375-402, 2017 Dec.
[Is] ISSN:1475-6374
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Respiratory syncytial virus (RSV) has been identified as a main cause of hospitalisation in infants and children. To date, the current therapeutic arsenal is limited to ribavirin and palivizumab with variable efficacy. In this work, starting from a number of in-house series of previously described anti-RSV agents based on the benzimidazole scaffold, with the aim at gaining a better understanding of the related chemical features involved in potency and safety profiles, we applied a computational study including two focussed comparative molecular fields analysis (CoMFA) and comparative molecular similarity indices analysis (CoMSIA). The results allowed us to derive useful suggestions for the design of derivatives and also to set up statistical models predicting the potency and selectivity index (SI = CC /EC ) of any new analogue prior to synthesis. Accordingly, here, we discuss preliminary results obtained through the applied exhaustive QSAR analyses, leading to design and synthesise more effective anti-RSV agents.
[Mh] MeSH terms primary: Antiviral Agents/pharmacology
Benzimidazoles/pharmacology
Respiratory Syncytial Viruses/drug effects
Respirovirus Infections/drug therapy
[Mh] MeSH terms secundary: Animals
Antiviral Agents/chemical synthesis
Antiviral Agents/chemistry
Benzimidazoles/chemical synthesis
Benzimidazoles/chemistry
Cell Line
Cercopithecus aethiops
Dose-Response Relationship, Drug
Humans
Microbial Sensitivity Tests
Models, Molecular
Molecular Structure
Quantitative Structure-Activity Relationship
Vero Cells
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antiviral Agents); 0 (Benzimidazoles); E24GX49LD8 (benzimidazole)
[Em] Entry month:1703
[Cu] Class update date: 170317
[Lr] Last revision date:170317
[Js] Journal subset:IM
[Da] Date of entry for processing:170310
[St] Status:MEDLINE
[do] DOI:10.1080/14756366.2016.1256881

  9 / 2909 MEDLINE  
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[PMID]: 28266286
[Au] Autor:Tsutsui R; Tsukagoshi H; Nagasawa K; Takahashi M; Matsushima Y; Ryo A; Kuroda M; Takami H; Kimura H
[Ad] Address:1​Aomori Prefecture Public Health and Environment Center, 1-1-1, Higashitsukurimichi, Aomori-shi, Aomori 030-8566, Japan 2​Department of Pathologic Analysis, Division of Medical Life Sciences, Hirosaki University Graduate School of Health Sciences, 66-1, Hon-cho, Hirosaki-shi, Aomori 036-8564, J
[Ti] Title:Genetic analyses of the fusion protein genes in human parainfluenza virus types 1 and 3 among patients with acute respiratory infections in Eastern Japan from 2011 to 2015.
[So] Source:J Med Microbiol;66(2):160-168, 2017 Feb.
[Is] ISSN:1473-5644
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:PURPOSE: To genetically explore the fusion protein gene (F) in human parainfluenza virus type 1 (HPIV1) and type 3 (HPIV3) strains, we analysed them in patients with acute respiratory infections in Eastern Japan from 2011 to 2015. METHODOLOGY: We constructed phylogenetic trees based on the HPIV and HPIV3 F gene using the maximum likelihood method and conducted P-distance and selective pressure analyses. We also predicted the linear epitopes of the protein in the prototype strains. Furthermore, we mapped the amino acid substitutions of the proteins. RESULTS: Nineteen strains of HPIV1 and 53 strains of HPIV3 were detected among the clinical acute respiratory infection cases. The phylogenetic trees indicated that the HPIV1 and HPIV3 strains were classified into clusters II and III and cluster C, respectively. The P-distance values of the HPIV1 and HPIV3 F genes were <0.03. Two positive selection sites were inferred in the HPIV1 (aa 8 and aa 10), and one positive selection site was inferred in the HPIV3 (aa 108), but over 10 negative selection sites were inferred. Four epitopes were predicted for the HPIV1 prototype strains, while five epitopes were predicted for the HPIV3 prototype strain. A positive selection site (aa 108) or the HPIV3 F protein was involved in the predicted epitope. Additionally, we found that an amino acid substitution (R73K) in the LC76627 HPIV3 strain presumably may affect the resistance to neutralization by antibodies. CONCLUSION: The F gene of HPIV1 and HPIV3 was relatively well conserved in the eastern part of Japan during the investigation period.
[Mh] MeSH terms primary: Parainfluenza Virus 1, Human/genetics
Parainfluenza Virus 3, Human/genetics
Respiratory Tract Infections/epidemiology
Respirovirus Infections/epidemiology
Viral Fusion Proteins/genetics
[Mh] MeSH terms secundary: Acute Disease
Adolescent
Adult
Aged
Aged, 80 and over
Amino Acid Substitution
Child
Child, Preschool
Epitopes/genetics
Epitopes/metabolism
Female
Humans
Infant
Japan/epidemiology
Likelihood Functions
Male
Middle Aged
Parainfluenza Virus 1, Human/isolation & purification
Parainfluenza Virus 3, Human/isolation & purification
Phylogeny
RNA, Viral/genetics
RNA, Viral/isolation & purification
Respiratory Tract Infections/virology
Respirovirus Infections/virology
Sequence Analysis, RNA
Viral Fusion Proteins/metabolism
Young Adult
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Epitopes); 0 (RNA, Viral); 0 (Viral Fusion Proteins); 109300-94-9 (F protein, parainfluenza virus 3)
[Em] Entry month:1703
[Cu] Class update date: 170315
[Lr] Last revision date:170315
[Js] Journal subset:IM
[Da] Date of entry for processing:170308
[St] Status:MEDLINE
[do] DOI:10.1099/jmm.0.000431

  10 / 2909 MEDLINE  
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[PMID]: 28146439
[Au] Autor:Hsu BS; Jones-Sapienza SA
[Ad] Address:From the Departments of *Pediatrics, and †Surgery, Sanford School of Medicine, University of South Dakota, Sioux Falls, SD.
[Ti] Title:Air in All the Wrong Places.
[So] Source:Pediatr Emerg Care;33(2):107-108, 2017 Feb.
[Is] ISSN:1535-1815
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Extraluminal air can occur through a wide variety of mechanisms. Often, the free air resides in isolated regions including the thorax, the peritoneum, or the mediastinum. We present a pediatric case where there was extensive extraluminal air simultaneously within several regions, one of which has never been reported in the literature.
[Mh] MeSH terms primary: Mediastinal Emphysema/diagnostic imaging
Pneumoperitoneum/diagnostic imaging
Pneumothorax/diagnostic imaging
Subcutaneous Emphysema/diagnostic imaging
[Mh] MeSH terms secundary: Female
Humans
Infant
Parainfluenza Virus 3, Human/isolation & purification
Respiratory Insufficiency/virology
Respirovirus Infections/complications
Respirovirus Infections/diagnosis
[Pt] Publication type:CASE REPORTS; JOURNAL ARTICLE
[Em] Entry month:1703
[Cu] Class update date: 170322
[Lr] Last revision date:170322
[Js] Journal subset:IM
[Da] Date of entry for processing:170202
[St] Status:MEDLINE
[do] DOI:10.1097/PEC.0000000000000539


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