Database : MEDLINE
Search on : Serratia and Infections [Words]
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[PMID]: 29499316
[Au] Autor:Sherry NL; Baines SL; Howden BP
[Ad] Address:Antimicrobial Reference and Resistance Unit, Microbiological Diagnostic Unit - Public Health Laboratory, Department of Microbiology & Immunology, University of Melbourne at the Peter Doherty Institute for Infection & Immunity, 792 Elizabeth Street, Melbourne, Victoria, Australia 3000; Department of Microbiology & Immunology, University of Melbourne at the Peter Doherty Institute for Infection & Immunity, 792 Elizabeth Street, Melbourne, Victoria, Australia 3000; Department of Infectious Diseases, Austin Health, 145 Studley Road, Heidelberg, Victoria, Australia 3084. Electronic address: nsherry@student.unimelb.edu.au.
[Ti] Title:Ceftazidime-avibactam susceptibility by three different susceptibility testing methods in carbapenemase-producing Gram-negative bacteria from Australia.
[So] Source:Int J Antimicrob Agents;, 2018 Feb 27.
[Is] ISSN:1872-7913
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Avibactam is a novel ß-lactamase inhibitor active against classes A, C and some class D ß-lactamases. In combination with ceftazidime, it may be useful for the treatment of infections due to carbapenemase-producing Gram negative (CPGN) bacteria from these classes; however, susceptibility data for some of the less-common carbapenemases are limited. To assess the in vitro activity of ceftazidime-avibactam (CZA), we tested a panel of fifty diverse CPGN collected from clinical isolates in Victoria, Australia, containing KPC, GES, SME, OXA-23 and OXA-48-like carbapenemases for CZA susceptibility using broth microdilution (BMD), E-tests and disc diffusion. All isolates were CZA susceptible. E-tests correlated well with BMD, although MICs were generally lower than BMD. Disc diffusion correlated moderately well with BMD, with two interpretive errors. Our study confirms phenotypic CZA susceptibility in the carbapenemase groups tested, including the less-common OXA-23-producing E. coli, SME-producing Serratia marcescens and GES-5-producing P. aeruginosa.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180302
[Lr] Last revision date:180302
[St] Status:Publisher

  2 / 3261 MEDLINE  
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[PMID]: 29212390
[Au] Autor:Selan L; Papa R; Ermocida A; Cellini A; Ettorre E; Vrenna G; Campoccia D; Montanaro L; Arciola CR; Artini M
[Ad] Address:1 Department of Public Health and Infectious Diseases, Sapienza University of Rome, Rome, Italy.
[Ti] Title:Serratiopeptidase reduces the invasion of osteoblasts by Staphylococcus aureus.
[So] Source:Int J Immunopathol Pharmacol;30(4):423-428, 2017 Dec.
[Is] ISSN:2058-7384
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Finding new strategies to counteract periprosthetic infection and implant failure is a main target in orthopedics. Staphylococcus aureus, the leading etiologic agent of orthopedic implant infections, is able to enter and kill osteoblasts, to stimulate pro-inflammatory chemokine secretion, to recruit osteoclasts, and to cause inflammatory osteolysis. Moreover, by entering eukaryotic cells, staphylococci hide from the host immune defenses and shelter from the extracellular antibiotics. Thus, infection persists, inflammation thrives, and a highly destructive osteomyelitis occurs around the implant. The ability of serratiopeptidase (SPEP), a metalloprotease by Serratia marcescens, to control S. aureus invasion of osteoblastic MG-63 cells and pro-inflammatory chemokine MCP-1 secretion was evaluated. Human osteoblast cells were infected with staphylococcal strains in the presence and in the absence of SPEP. Cell proliferation and cell viability were also evaluated. The release of pro-inflammatory chemokine MCP-1 was evaluated after the exposure of the osteoblast cells to staphylococcal strains. The significance of the differences in the results of each test and the relative control values was determined with Student's t-test. SPEP impairs their invasiveness into osteoblasts, without affecting the viability and proliferation of bone cells, and tones down their production of MCP-1. We recognize SPEP as a potential tool against S. aureus bone infection and destruction.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180301
[Lr] Last revision date:180301
[St] Status:In-Process
[do] DOI:10.1177/0394632017745762

  3 / 3261 MEDLINE  
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[PMID]: 29475568
[Au] Autor:Lepelletier D; Bonnet R; Plésiat P; Nicolas-Chanoine MH; Berger-Carbonne A; Chidiac C; Grandbastien B; national working group from the French High Council of Public Health
[Ad] Address:Service de bactériologie, hygiène hospitalière, CHU de Nantes, 5, rue du Professeur-Yves-Boquien, 44093 Nantes, France; Laboratoire de recherche MihAR, UFR médecine, université de Nantes, 44200 Nantes, France; Commission spécialisée sécurité patient, haut conseil de la santé publique, 75014 Paris, F
[Ti] Title:Emergence of plasmid-mediated colistin resistance (mcr-1) among Enterobacteriaceae strains: Laboratory detection of resistance and measures to control its dissemination.
[So] Source:Med Mal Infect;, 2018 Feb 20.
[Is] ISSN:1769-6690
[Cp] Country of publication:France
[La] Language:eng
[Ab] Abstract:The increasing use of colistin has contributed to the emergence of resistant bacteria and to an increase in the frequency of infections caused by naturally resistant Enterobacteriaceae strains such as Proteus, Providencia, Morganella, and Serratia. In August 2016, the French High Council for Public Health (French acronym HCSP) received a request from the Ministry of Health on the advice of the French National Public Health agency (Santé publique France) with regard to measures that should be taken to tackle the emergence of plasmid-mediated colistin resistance among Enterobacteriaceae strains. French healthcare facilities were asked to take the necessary measures as soon as possible, such as updating the definition of emerging highly resistant bacteria and defining the identification methods so as to take account of the evolving epidemiology of this type of resistance. This article describes the epidemiological context of the discovery of this emergence in France and worldwide, the resistance mechanisms, the microbiological methods of routine laboratory detection and the level of hygiene measures to implement in French facilities.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180224
[Lr] Last revision date:180224
[St] Status:Publisher

  4 / 3261 MEDLINE  
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[PMID]: 29203405
[Au] Autor:Baron S; Leulmi Z; Villard C; Olaitan AO; Telke AA; Rolain JM
[Ad] Address:Aix-Marseille Université, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Facultés de Médecine et de Pharmacie, 19-21 bd Jean Moulin, Marseille, France.
[Ti] Title:Inactivation of the arn operon and loss of aminoarabinose on lipopolysaccharide as the cause of susceptibility to colistin in an atypical clinical isolate of proteus vulgaris.
[So] Source:Int J Antimicrob Agents;, 2017 Dec 01.
[Is] ISSN:1872-7913
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Colistin has become a last-line antibiotic for the treatment of multidrug-resistant bacterial infections; however, resistance to colistin has emerged in recent years. Some bacteria, such as Proteus and Serratia spp., are intrinsically resistant to colistin although the exact mechanism of resistance is unknown. Here we identified the molecular support for intrinsic colistin resistance in Proteus spp. by comparative genomic, transcriptomic and proteomic analyses of colistin-susceptible (CSUR P1868_S) and colistin-resistant (CSUR P1867_R) strains of an atypical Proteus vulgaris. A significant difference in outer membrane glycoside structures in both strains that was corroborated by MALDI-TOF/MS analysis was found, which showed an absence of 4-amino-4-deoxy-l-arabinose (L-Ara4N) in the outer membrane lipid A moiety of the susceptible strain. Comparative genomic analysis with other resistant strains of P. vulgaris available in a local database found a mutation in the arnBCADTEF operon of the susceptible strain. Transcriptomic analysis of genes belonging to the arnBCADTEF operon showed a significant decrease in mRNA expression level of these genes in the susceptible strain, supporting addition of L-Ara4N in the outer membrane lipid A moiety as an explanation for colistin resistance. Insertion of the arnD gene that was suggested to be altered in the susceptible strain by in silico analysis led to a 16-fold increase of colistin MIC in the susceptible strain, confirming its role in colistin resistance in this species. Here we show that constitutive activation of the arn operon and addition of L-Ara4N is the main molecular mechanism of colistin resistance in P. vulgaris.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180223
[Lr] Last revision date:180223
[St] Status:Publisher

  5 / 3261 MEDLINE  
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[PMID]: 29460560
[Au] Autor:Elli S; Cannizzo L; Foti G; Fumagalli R; Lucchini A
[Ad] Address:Infermiere, Terapia intensiva generale, ASST Monza - ospedale S.Gerardo - s.elli@asst-monza.it.
[Ti] Title:Misure di isolamento per infezioni da microorganismi multiresistenti e carico di lavoro infermieristico in una terapia intensiva polivalente. [Isolation precautions in multi drug resistent infections and nursing workload in a general intensive care unit].
[So] Source:Prof Inferm;70(4):231-237, 2017 Oct-Dec.
[Is] ISSN:0033-0205
[Cp] Country of publication:Italy
[La] Language:ita
[Ab] Abstract:BACKGROUND: Critically ill patients in ICU are exposed to high risk of hospital acquired infections. In recent years, the multi drug resistant microorganisms (MDR) represent the most worrying epidemiological problem. AIM: The aim of this study is to evaluate the relationship between isolation precautions and nursing workload. METHODS: We studied patients who had an infection by MDR, subject to isolation precautions, and measured their NAS score during stay in ICU. MDR infections of studied patients were: Acinetobacter Baumannii, Klebsiella KPC, MRSA, Pseudomonas, Escherichia coli, Serratia marcescens e Clostridium difficile. Isolation precutions wer identified by color code (green, yellow, red). RESULTS: We studied 44 patients during the year 2012. NAS average was 81.54 ± 10.25. NAS average for "green code" patients was 81.25 ± 22.12, for "yellow code" patients was 82.57 ± 11.25 and for "red code" patients was 79.06 ± 29.12. DISCUSSION: the presence of isolation precautions seems to have no influence on nursing workload measured by NAS score, except for Acinetobacter Baumannii infection. Further research will be needed for better evaluation of this topic.
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180220
[Lr] Last revision date:180220
[St] Status:In-Data-Review
[do] DOI:10.7429/pi.2017.704231

  6 / 3261 MEDLINE  
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[PMID]: 29457249
[Au] Autor:Akbar N; Siddiqui R; Iqbal M; Sagathevan K; Khan NA
[Ad] Address:Department of Biological Sciences, School of Science and Technology Sunway University, Malaysia.
[Ti] Title:Gut bacteria of cockroaches are a potential source of antibacterial compound(s).
[So] Source:Lett Appl Microbiol;, 2018 Feb 19.
[Is] ISSN:1472-765X
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Here, we hypothesized that the microbial gut flora of animals/pests living in polluted environments, produce substances to thwart bacterial infections. The overall aim of this study was to source microbes inhabiting unusual environmental niches for potential antimicrobial activity. Two cockroach species, Gromphadorhina portentosa (Madagascar) and Blaptica dubia (Dubia) were selected. The gut bacteria from these species were isolated and grown in RPMI 1640 and conditioned media were prepared. Conditioned media were tested against a panel of Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pyogenes, Bacillus cereus) and Gram-negative (Escherichia coli K1, Salmonella enterica, Serratia marcescens, Pseudomonas aeruginosa, Klebsiella pneumoniae) bacteria, as well as the protist pathogen, Acanthamoeba castellanii. The results revealed that the gut bacteria of cockroaches produce active molecule(s) with potent antibacterial properties, as well as exhibit antiamoebic effects. However, heat-inactivation at 95°C for 10 min had no effect on conditioned media-mediated antibacterial and antiamoebic properties. These results suggest that bacteria from novel sources i.e., from the cockroach's gut produce molecules with bactericidal as well as amoebicidal properties that can ultimately lead to the development of therapeutic drugs. This article is protected by copyright. All rights reserved.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180219
[Lr] Last revision date:180219
[St] Status:Publisher
[do] DOI:10.1111/lam.12867

  7 / 3261 MEDLINE  
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[PMID]: 29254478
[Au] Autor:Zhang H; Yang Q; Liao K; Ni Y; Yu Y; Hu B; Sun Z; Huang W; Wang Y; Wu A; Feng X; Luo Y; Chu Y; Chen S; Cao B; Su J; Duan Q; Zhang S; Shao H; Kong H; Gui B; Hu Z; Badal R; Xu Y
[Ad] Address:Division of Microbiology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No. 1 Shuaifuyuan, Wangfujing Street, Beijing, 100730, China.
[Ti] Title:Update of incidence and antimicrobial susceptibility trends of Escherichia coli and Klebsiella pneumoniae isolates from Chinese intra-abdominal infection patients.
[So] Source:BMC Infect Dis;17(1):776, 2017 12 18.
[Is] ISSN:1471-2334
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: To evaluate in vitro susceptibilities of aerobic and facultative Gram-negative bacterial (GNB) isolates from intra-abdominal infections (IAIs) to 12 selected antimicrobials in Chinese hospitals from 2012 to 2014. METHODS: Hospital acquired (HA) and community acquired (CA) IAIs were collected from 21 centers in 16 Chinese cities. Extended spectrum beta-lactamase (ESBL) status and antimicrobial susceptibilities were determined at a central laboratory using CLSI broth microdilution and interpretive standards. RESULTS: From all isolated strains the Enterobacteriaceae (81.1%) Escherichia coli accounted for 45.4% and Klebsiella pneumoniae for 20.1%, followed by Enterobacter cloacae (5.2%), Proteus mirabilis (2.1%), Citrobacter freundii (1.8%), Enterobacter aerogenes (1.8%), Klebsiella oxytoca (1.4%), Morganella morganii (1.2%), Serratia marcescens (0.7%), Citrobacter koseri (0.3%), Proteus vulgaris (0.3%) and others (1.0%). Non- Enterobacteriaceae (18.9%) included Pseudomonas aeruginosa (9.8%), Acinetobacter baumannii (6.7%), Stenotrophomonas maltophilia (0.9%), Aeromonas hydrophila (0.4%) and others (1.1%). ESBL-screen positive Escherichia coli isolates (ESBL+) showed a decreasing trend from 67.5% in 2012 to 58.9% in 2014 of all Escherichia coli isolates and the percentage of ESBL+ Klebsiella pneumoniae isolates also decreased from 2012 through 2014 (40.4% to 26.6%), which was due to reduced percentages of ESBL+ isolates in HA IAIs for both bacteria. The overall susceptibilities of all 5160 IAI isolates were 87.53% to amikacin (AMK), 78.12% to piperacillin-tazobactam (TZP) 81.41% to imipenem (IMP) and 73.12% to ertapenem (ETP). The susceptibility of ESBL-screen positive Escherichia coli strains was 96.77%-98.8% to IPM, 91.26%-93.16% to ETP, 89.48%-92.75% to AMK and 84.86%-89.34% to TZP, while ESBL-screen positive Klebsiella pneumoniae strains were 70.56%-80.15% susceptible to ETP, 80.0%-87.5% to IPM, 83.82%-87.06% to AMK and 63.53%-68.38% to TZP within the three year study. Susceptibilities to all cephalosporins and fluoroquinolones were less than 50% beside 66.5% and 56.07% to cefoxitin (FOX) for ESBL+ Escherichia coli and Klebsiella pneumoniae strains respectively. CONCLUSIONS: The total ESBL+ rates decreased in Escherichia coli and Klebsiella pneumoniae IAI isolates due to fewer prevalence in HA infections. IPM, ETP and AMK were the most effective antimicrobials against ESBL+ Escherichia coli and Klebsiella pneumoniae IAI isolates in 2012-2014 and a change of fluoroquinolone regimens for Chinese IAIs is recommended.
[Mh] MeSH terms primary: Abdomen/microbiology
Anti-Bacterial Agents/pharmacology
Escherichia coli Infections/microbiology
Escherichia coli/drug effects
Klebsiella Infections/microbiology
Klebsiella pneumoniae/drug effects
[Mh] MeSH terms secundary: Cephalosporins/pharmacology
China/epidemiology
Community-Acquired Infections/epidemiology
Community-Acquired Infections/microbiology
Cross Infection/microbiology
Escherichia coli/classification
Escherichia coli/genetics
Escherichia coli/isolation & purification
Escherichia coli Infections/epidemiology
Humans
Imipenem/pharmacology
Incidence
Intraabdominal Infections/microbiology
Klebsiella Infections/epidemiology
Klebsiella pneumoniae/classification
Klebsiella pneumoniae/genetics
Klebsiella pneumoniae/isolation & purification
Microbial Sensitivity Tests
beta-Lactams/pharmacology
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Anti-Bacterial Agents); 0 (Cephalosporins); 0 (beta-Lactams); 71OTZ9ZE0A (Imipenem); G32F6EID2H (ertapenem)
[Em] Entry month:1801
[Cu] Class update date: 180214
[Lr] Last revision date:180214
[Js] Journal subset:IM
[Da] Date of entry for processing:171220
[St] Status:MEDLINE
[do] DOI:10.1186/s12879-017-2873-z

  8 / 3261 MEDLINE  
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[PMID]: 29223516
[Au] Autor:Martins AF; Bail L; Ito CAS; da Silva Nogueira K; Dalmolin TV; Martins AS; Rocha JLL; Serio AW; Tuon FF
[Ad] Address:Division of Microbiology, Universidade Federal do Rio Grande do Sul.
[Ti] Title:Antimicrobial activity of plazomicin against Enterobacteriaceae-producing carbapenemases from 50 Brazilian medical centers.
[So] Source:Diagn Microbiol Infect Dis;90(3):228-232, 2018 Mar.
[Is] ISSN:1879-0070
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Plazomicin is a next-generation aminoglycoside with activity against Enterobacteriaceae, including carbapenemase-producing Enterobacteriaceae (CPE). The aim of this study was to evaluate the activity of plazomicin against CPE (Klebsiella spp., Escherichia coli, Serratia spp., Enterobacter spp., Citrobacter spp., Morganella spp., Proteus spp., Providencia spp.) from different Brazilian hospitals. A total of 4000 carbapenem-resistant Enterobacteriaceae isolates were collected from clinical samples in 50 Brazilian hospitals during 2013-2015. Of these, 499 carbapenem-resistant isolates (CLSI criteria) were selected for further evaluation via broth microdilution to assess for the activity of plazomicin, colistin, tigecycline, meropenem, amikacin, and gentamicin. Additionally, the isolates were assessed for the presence of carbapenemase genes (bla , bla , bla , bla , bla , bla , and bla ) by polymerase chain reaction (PCR). When PCR was positive to bla , bla , bla , and bla the carbapenemase genes were sequenced. bla was the most prevalent carbapenemase gene found (n=397), followed by bla (n=81), bla (n=12), and bla (n=3). Other genes were identified in only 1 isolate each: bla , bla , bla , bla , and bla . One isolate had 2 carbapenemase genes (bla and bla ). Thirty-three percent of the isolates were nonsusceptible to colistin, 24% to tigecycline, 97% to meropenem, 51% to amikacin, and 81% to gentamicin (via EUCAST criteria). The plazomicin MIC was 0.5/64mg/L, with 85% of MICs ≤2mg/L and 87% of MICs ≤4mg/L. Elevated MICs to plazomicin were not associated with a specific carbapenemase or bacterial species. The MICs of plazomicin against CPE were lower than those of other aminoglycosides. Plazomicin is a promising drug for the treatment of CPE infections.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180213
[Lr] Last revision date:180213
[St] Status:In-Process

  9 / 3261 MEDLINE  
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[PMID]: 29431875
[Au] Autor:Ravindran D; Ramanathan S; Arunachalam K; Jeyaraj GP; Shunmugiah KP; Arumugam VR
[Ad] Address:Department of Biotechnology Science Campus, Alagappa University, Karaikudi, 630 003, India.
[Ti] Title:Phytosynthesized silver nanoparticles as anti-quorum sensing and antibiofilm agent against the nosocomial pathogen Serratia marcescens: an in vitro study.
[So] Source:J Appl Microbiol;, 2018 Feb 12.
[Is] ISSN:1365-2672
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:AIM: Serratia marcescens is an important multidrug-resistant human pathogen. The pathogenicity of S. marcescens mainly depends on the Quorum Sensing (QS) mechanism, which regulates the virulence factors production and biofilm formation. Hence, targeting QS mechanism in S. marcescens will ultimately pave the way to combat its pathogenicity. Thus, the present study is intended to evaluate the efficacy of Vetiveria zizanioides root extract mediated silver nanoparticles (AgNPs) as a potent anti-QS and antibiofilm agent against S. marcescens. METHODS AND RESULTS: The AgNPs were synthesized using V. zizanioides aqueous root extract and the physiochemical properties of V. zizanioides based AgNPs (VzAgNPs) were evaluated using analytical techniques such as ultraviolet-visible absorption spectroscopy, X-ray diffraction, fourier transform infrared spectroscopy, dynamic light scattering and scanning and transmission electron microscopic techniques. VzAgNPs were found to attenuate the QS-dependent virulence factors, namely prodigiosin, protease, lipase, exopolysaccharide productions and biofilm formation of S. marcescens, without inhibiting its growth. Further, the transcriptomic analysis confirmed the down regulation of QS dependent genes, which encode for the production of virulence factors and biofilm formation. CONCLUSION: The current study confirms VzAgNPs as an ideal anti-QS and antibiofilm agent against S. marcescens. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first approach that validates the anti-QS and antibiofilm potential of phytosynthesized VzAgNPs against the nosocomial pathogen, S. marcescens. As VzAgNPs exhibits potent antivirulent activities, it could be used to treat hospital acquired S. marcescens infections. This article is protected by copyright. All rights reserved.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180212
[Lr] Last revision date:180212
[St] Status:Publisher
[do] DOI:10.1111/jam.13728

  10 / 3261 MEDLINE  
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[PMID]: 29341217
[Au] Autor:Horne SM; Schroeder M; Murphy J; Prüß BM
[Ad] Address:Department of Microbiological Sciences, North Dakota State University, Fargo, ND, USA.
[Ti] Title:Acetoacetate and ethyl acetoacetate as novel inhibitors of bacterial biofilm.
[So] Source:Lett Appl Microbiol;, 2018 Jan 16.
[Is] ISSN:1472-765X
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Acetoacetate (AAA) was identified as a biofilm inhibitor in a previous study, where the effect of 190 carbon and nitrogen sources on biofilm amounts by Escherichia coli O157:H7 was determined. With this study, we tested the effect of AAA on growth and biofilm amounts of Cronobacter sakazakii, Serratia marcescens and Yersinia enterocolitica. AAA reduced growth and biofilm amounts of the three pathogens, albeit at rather high concentrations of 10 to 35 mg ml . Acetoacetate at a concentration of 5 mg ml reduced Y. enterocolitica mRNA transcripts of the flagellar master regulator operon flhD, the invasion gene inv, and the adhesion gene yadA. Transcription of the regulator of plasmid-encoded virulence genes virF, the plasmid-encoded virulence gene yopQ, and ymoA were largely unaffected by AAA. Importantly, AAA did not cause an increase in transcription of any of the tested virulence genes. As a more cost efficient homologue of AAA, the effect of ethyl acetoacetate (EAA) was tested. EAA reduced growth, biofilm amounts and live bacterial cell counts up to 3 logs. IC values ranged from 0·31 mg ml to 5·6 mg ml . In summary, both AAA and EAA inhibit biofilm, but EAA appears to be more effective. SIGNIFICANCE AND IMPACT OF THE STUDY: Bacterial biofilms are communities of bacteria that form on surfaces and are extremely difficult to remove by conventional physical or chemical techniques, antibiotics or the human immune system. Despite advanced technologies, biofilm still contributes to 60 to 80% of human bacterial infections (NIH and CDC) and cause problems in many natural, environmental, bioindustrial or food processing settings. The discovery of novel substances that inhibit biofilm without increasing the virulence of the bacteria opens doors for countless applications where a reduction of biofilm is desired.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180209
[Lr] Last revision date:180209
[St] Status:Publisher
[do] DOI:10.1111/lam.12852


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