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[PMID]: 29149807
[Au] Autor:Shu J; Li L; Zhou M; Yu J; Peng C; Shao F; Liu R; Zhu G; Huang H
[Ad] Address:a Key Laboratory of Modern Preparation of TCM , Jiangxi University of Traditional Chinese Medicine, Ministry of Education , Nanchang , China.
[Ti] Title:Three new flavonoid glycosides from Smilax glabra and their anti-inflammatory activity.
[So] Source:Nat Prod Res;:1-9, 2017 Nov 17.
[Is] ISSN:1478-6427
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Three new flavonoid glycosides, 2(S)-5-hydroxy-6,8-dimethoxyflavonone-7-O-ß-D-glucopyranosyl-(1→6)-O-ß-D-glucopyranoside (1), 5-hydroxy-3,8-dimethoxyflavone-7-O-ß-D-glucopyranosyl-(1→6)-O-ß-D-glucopyranoside (2) and 3,7-dihydroxy-8-methoxyflavone-6-O-ß-D-glucopyranosyl-(1→6)-O-ß-D-glucopyranoside (3), together with five known flavonoids (4-8) were isolated from the roots of Smilax glabra Roxb. Their structures were elucidated on the basis of chemical and spectral evidence, as well as by comparison with literature data. Three new flavonoids were subjected to evaluate anti-inflammatory activity. Compounds 1-3 inhibited the NF κB induction by 32.2, 55.8 and 61.7%, respectively.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1711
[Cu] Class update date: 171118
[Lr] Last revision date:171118
[St] Status:Publisher
[do] DOI:10.1080/14786419.2017.1402314

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[PMID]: 28624472
[Au] Autor:Gao Y; Li C; Wang Y; Liu Y; Li G; Fan X; Li Y; Tian J; Lee AW
[Ad] Address:School of Life Sciences, Yantai University, Yantai, 264005, PR China.
[Ti] Title:Nonclinical safety of astilbin: A 4-week oral toxicity study in rats with genotoxicity, chromosomal aberration, and mammalian micronucleus tests.
[So] Source:Food Chem Toxicol;107(Pt A):1-9, 2017 Sep.
[Is] ISSN:1873-6351
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Astilbin is an active flavonoid compound isolated from Rhizoma Smilacis Glabrae. It has been widely used as an anti-hepatic, anti-arthritic, and anti-renal injury agent. However, its safety has not yet been established. The objective of this study was to evaluate 4-week repeated oral toxicity and genotoxicity of astilbin. We examined oral toxicity in Sprague-Dawley rats after daily oral administration of astilbin at 50, 150, and 500 mg/kg for 4 weeks. Negative control animals received the same volume of the solvent. Astilbin administration did not lead to death, body weight gain, food consumption, or adverse events. There were no significant differences in toxicity between the astilbin and control group; we observed no toxic effects on hematological or urinalysis parameters, biochemical values, organ weight, or histopathological findings. We assessed the genotoxicity of astilbin with the Ames test (TA97a, TA98, TA100, TA102, and TA1535), chromosomal aberration assay (using Chinese hamster ovary cells), and mammalian micronucleus test (in mice). We found no genotoxicity in any tested strains. The no-observed-adverse-effect level (NOAEL) for astilbin in the 4-week repeated oral toxicity study in rats was greater than 500 mg/kg body weight/day, regardless of gender. Results also suggested that astilbin does not have genotoxicity potential.
[Mh] MeSH terms primary: Chromosome Aberrations/drug effects
Drugs, Chinese Herbal/adverse effects
Flavonols/adverse effects
Smilacaceae/chemistry
[Mh] MeSH terms secundary: Animals
CHO Cells
Cricetinae
Cricetulus
DNA Damage/drug effects
Female
Flavonols/administration & dosage
Male
Mice
Micronucleus Tests
Mutagenicity Tests
No-Observed-Adverse-Effect Level
Plant Extracts/administration & dosage
Plant Extracts/chemistry
Rats
Rats, Sprague-Dawley
Salmonella typhimurium/drug effects
Salmonella typhimurium/genetics
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Drugs, Chinese Herbal); 0 (Flavonols); 0 (Plant Extracts); 29838-67-3 (astilbin)
[Em] Entry month:1708
[Cu] Class update date: 170829
[Lr] Last revision date:170829
[Js] Journal subset:IM
[Da] Date of entry for processing:170619
[St] Status:MEDLINE

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[PMID]: 28504017
[Au] Autor:Amado PA; Ferraz V; da Silva DB; Carollo CA; Castro AHF; Alves Rodrigues Dos Santos Lima L
[Ad] Address:a Campus Centro-Oeste Dona Lindu , Federal University of São João Del-Rei , Divinópolis , Brazil.
[Ti] Title:Chemical composition, antioxidant and cytotoxic activities of extracts from the leaves of Smilax brasiliensis Sprengel (Smilacaceae).
[So] Source:Nat Prod Res;:1-6, 2017 May 15.
[Is] ISSN:1478-6427
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:The antioxidant and cytotoxic activities of petroleum ether and methanol extracts, fatty acids and methyl esters from leaves of Smilax brasiliensis were evaluated, and the composition of the extracts was determined. Palmitic, linoleic and linolenic acids were major components of the extracts. For antioxidant activity, all samples exhibited IC values lower than BHT (2,6-di-tert-butyl-4-methylphenol). The extracts, fatty acids and methyl esters from S. brasiliensis presented no toxicity to larvae of the brine shrimp, Artemia salina. Among the purified substances, only methyl linolenate showed toxicity (LD = 21.47 µg/mL). This study showed, for the first time, the composition of petroleum ether and methanol extracts from S. brasiliensis leaves, as well as the antioxidant and cytotoxic activities of extracts, fatty acids and methyl esters.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1705
[Cu] Class update date: 170622
[Lr] Last revision date:170622
[St] Status:Publisher
[do] DOI:10.1080/14786419.2017.1327861

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[PMID]: 28439478
[Au] Autor:Qi ZC; Shen C; Han YW; Shen W; Yang M; Liu J; Liang ZS; Li P; Fu CX
[Ad] Address:College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou 310018, People's Republic of China.
[Ti] Title:Development of microsatellite loci in Mediterranean sarsaparilla ( ; Smilacaceae) using transcriptome data.
[So] Source:Appl Plant Sci;5(4), 2017 Apr.
[Is] ISSN:2168-0450
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PREMISE OF THE STUDY: Although several microsatellite markers of (Smilacaceae) have been reported in a previous study, due to universality issues in cross-population amplification, we have newly developed microsatellite markers for based on transcriptome data to further investigate gene flow and genetic structure of its circum-Mediterranean, East African, and South Asian populations. METHODS AND RESULTS: A total of 4854 simple sequence repeat (SSR) primer pairs were designed from 99,193 contigs acquired from public transcriptome data of . Forty-six microsatellite loci were selected for further genotyping in 12 populations. The number of alleles varied from three to 28, and 93.5% of the developed microsatellite markers could be cross-amplified in least one of three congeneric species. CONCLUSIONS: The SSR markers developed in this study will facilitate further studies on genetic diversity and phylogeographic patterns of in intercontinental geographical scales.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1704
[Cu] Class update date: 170428
[Lr] Last revision date:170428
[St] Status:PubMed-not-MEDLINE

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[PMID]: 28337395
[Au] Autor:Ru Y; Cheng R; Shang J; Zhao Y; Li P; Fu C
[Ad] Address:Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, College of Life Sciences, Zhejiang University, Hangzhou 310058, People's Republic of China; Laboratory of Systematic and Evolutionary Botany and Biodiversity, Institute of Ecology and Conservation Center for
[Ti] Title:Isolation and characterization of microsatellite loci for (Smilacaceae).
[So] Source:Appl Plant Sci;5(3), 2017 Mar.
[Is] ISSN:2168-0450
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PREMISE OF THE STUDY: Polymorphic microsatellite markers were developed for (Smilacaceae), a member of the group with a biogeographic disjunction between eastern Asia and North America, to study the phylogeography and incipient speciation of this species and its close relatives. METHODS AND RESULTS: Transcriptome sequencing produced 47,628 unigenes. Seventeen loci were developed from 122 randomly selected primer pairs. Polymorphism and genetic variation were evaluated for 68 accessions representing five populations of . The number of alleles per locus ranged from four to 18; the expected heterozygosity ranged from 0.59 to 0.92. Twelve loci were successfully amplified in five related species: , , , , and . CONCLUSIONS: These novel expressed sequence tag-derived microsatellite markers will facilitate further population genetic research of and its close allies of the group.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1703
[Cu] Class update date: 170403
[Lr] Last revision date:170403
[St] Status:PubMed-not-MEDLINE

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[PMID]: 28287485
[Au] Autor:Lee HE; Kim JA; Whang WK
[Ad] Address:Pharmaceutical Botany Laboratory, College of Pharmacy, Chung-Ang University, Heukseok-dong, Dongjak-gu, Seoul 156-756, Korea. y-u-h-r214@hanmail.net.
[Ti] Title:Chemical Constituents of Smilax china L. Stems and Their Inhibitory Activities against Glycation, Aldose Reductase, α-Glucosidase, and Lipase.
[So] Source:Molecules;22(3), 2017 Mar 11.
[Is] ISSN:1420-3049
[Cp] Country of publication:Switzerland
[La] Language:eng
[Ab] Abstract:The search for natural inhibitors with anti-diabetes properties has gained increasing attention. Among four selected Smilacaceae family plants, L. stems (SCS) showed significant in vitro anti-glycation and rat lens aldose reductase inhibitory activities. Bioactivity-guided isolation was performed with SCS and four solvent fractions were obtained, which in turn yielded 10 compounds, including one phenolic acid, three chlorogenic acids, four flavonoids, one stilbene, and one phenylpropanoid glycoside; their structures were elucidated using nuclear magnetic resonance and mass spectrometry. All solvent fractions, isolated compounds, and stem extracts from plants sourced from six different provinces of South Korea were next tested for their inhibitory effects against advanced glycation end products, as well as aldose reductase. α-Glucosidase, and lipase assays were also performed on the fractions and compounds. Since compounds , , , and appeared to be the superior inhibitors among the tested compounds, a comparative study was performed via high-performance liquid chromatography with photodiode array detection using a self-developed analysis method to confirm the relationship between the quantity and bioactivity of the compounds in each extract. The findings of this study demonstrate the potent therapeutic efficacy of SCS and its potential use as a cost-effective natural alternative medicine against type 2 diabetes and its complications.
[Mh] MeSH terms primary: Aldehyde Reductase/antagonists & inhibitors
Chlorogenic Acid/chemistry
Glycation End Products, Advanced/antagonists & inhibitors
Glycosides/chemistry
Hydroxybenzoates/chemistry
Smilax/chemistry
Stilbenes/chemistry
[Mh] MeSH terms secundary: Aldehyde Reductase/metabolism
Animals
Chlorogenic Acid/isolation & purification
Chlorogenic Acid/pharmacology
Ethanol/chemistry
Flavonoids/chemistry
Flavonoids/isolation & purification
Flavonoids/pharmacology
Glycation End Products, Advanced/metabolism
Glycosides/isolation & purification
Glycosides/pharmacology
Glycosylation/drug effects
Hydroxybenzoates/isolation & purification
Hydroxybenzoates/pharmacology
Lens, Crystalline/chemistry
Lens, Crystalline/enzymology
Lipase/antagonists & inhibitors
Lipase/metabolism
Methanol/chemistry
Molecular Structure
Plant Extracts/chemistry
Plant Stems/chemistry
Rats
Rats, Sprague-Dawley
Solvents/chemistry
Stilbenes/isolation & purification
Stilbenes/pharmacology
alpha-Glucosidases/metabolism
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Flavonoids); 0 (Glycation End Products, Advanced); 0 (Glycosides); 0 (Hydroxybenzoates); 0 (Plant Extracts); 0 (Solvents); 0 (Stilbenes); 29656-58-4 (phenolic acid); 318ADP12RI (Chlorogenic Acid); 3K9958V90M (Ethanol); EC 1.1.1.21 (Aldehyde Reductase); EC 3.1.1.3 (Lipase); EC 3.2.1.20 (alpha-Glucosidases); Y4S76JWI15 (Methanol)
[Em] Entry month:1705
[Cu] Class update date: 171116
[Lr] Last revision date:171116
[Js] Journal subset:IM
[Da] Date of entry for processing:170314
[St] Status:MEDLINE

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[PMID]: 28276765
[Au] Autor:Liu X; Liang J; Pan LL; Chen JY; Liu RH; Zhu GH; Huang HL; Shu JC; Shao F; Liang YH; Yu JL
[Ad] Address:a Key Laboratory of Modern Preparation of TCM , Ministry of Education, Jiangxi University of Traditional Chinese Medicine , Nanchang 330004 , China.
[Ti] Title:Six new furostanol glycosides from Smilax glauco-china and their cytotoxic activity.
[So] Source:J Asian Nat Prod Res;19(8):754-765, 2017 Aug.
[Is] ISSN:1477-2213
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Six new steroidal saponins, namely glauco-chinaosides A-F, and one known compound were isolated from the tubers of Smilax glauco-china. Their structures were elucidated by a combination of spectroscopic analysis and hydrolysis followed by spectral and chromatographic analysis. Compounds 1-7 were tested in vitro for their cytotoxic activities against four human tumor cell lines (SH-SY5Y, SGC-7901, HCT-116, and Lovo). Compounds 1, 2, and 5 exhibited cytotoxic activity against SGC-7901, with IC values of 2.7, 11.5, and 6.8 µM, respectively.
[Mh] MeSH terms primary: Antineoplastic Agents, Phytogenic/isolation & purification
Antineoplastic Agents, Phytogenic/pharmacology
Drugs, Chinese Herbal/isolation & purification
Drugs, Chinese Herbal/pharmacology
Glycosides/isolation & purification
Glycosides/pharmacology
Saponins/isolation & purification
Saponins/pharmacology
Smilax/chemistry
Sterols/isolation & purification
Sterols/pharmacology
[Mh] MeSH terms secundary: Antineoplastic Agents, Phytogenic/chemistry
Drug Screening Assays, Antitumor
Drugs, Chinese Herbal/chemistry
Glycosides/chemistry
HCT116 Cells
Humans
Inhibitory Concentration 50
Molecular Structure
Nuclear Magnetic Resonance, Biomolecular
Rhizome/chemistry
Saponins/chemistry
Sterols/chemistry
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antineoplastic Agents, Phytogenic); 0 (Drugs, Chinese Herbal); 0 (Glycosides); 0 (Saponins); 0 (Sterols); 0 (furostanol glycoside)
[Em] Entry month:1709
[Cu] Class update date: 170901
[Lr] Last revision date:170901
[Js] Journal subset:IM
[Da] Date of entry for processing:170310
[St] Status:MEDLINE
[do] DOI:10.1080/10286020.2017.1281913

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[PMID]: 28101120
[Au] Autor:Hirota BC; Paula CD; de Oliveira VB; da Cunha JM; Schreiber AK; Ocampos FM; Barison A; Miguel OG; Miguel MD
[Ad] Address:Pharmacotechnical Laboratory, Department of Pharmacy, Universidade Federal do Paraná, 80.210-170 Curitiba, PR, Brazil.
[Ti] Title:Phytochemical and Antinociceptive, Anti-Inflammatory, and Antioxidant Studies of (Smilacaceae).
[So] Source:Evid Based Complement Alternat Med;2016:9894610, 2016.
[Is] ISSN:1741-427X
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The tea of aerial parts of Griseb. (Smilacaceae) has been ethnopharmacologically used in Southern Brazil due to its anti-inflammatory action. In this study, ethanolic and organic extracts from aerial parts of were phytochemically and pharmacologically characterized. The phytochemical analysis of EtOAc extract of revealed the presence of three flavonoids, drabanemoroside, kaempferol 3- - -L-rhamnopyranosyl(1→2)- -L-rhamnopyranoside, and kaempferol, the first two being isolated for the first time in this genus, two phenolic compounds hydroxybenzoic acid and coumaric acid, and alkaloids. In vitro assays demonstrated a potential antioxidant property of SLG. The treatment with SLG induced a significant reduction of the formalin-evoked flinches in rats, an effect reversed by opioid antagonist naloxone. Treatment with SLG also induced a significant increase in the hot plate latency and a decrease of intestinal motility by 45%. No effect was observed over nociceptive responses induced by a TRPA1 agonist mustard oil or over acetic acid-induced writhing in mice. Together, our data suggested that SLG has an in vivo antinociceptive effect, which seems to be associated with the opioid system activation. These findings support previous claims of medical use of in the treatment of pain conditions.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1701
[Cu] Class update date: 170816
[Lr] Last revision date:170816
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1155/2016/9894610

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[PMID]: 27323613
[Au] Autor:Zhang Q; Li T; Xu YG; Yang XH
[Ti] Title:[Effect of Chinese Herbs Used in Treating Multiple Sclerosis on T Subsets Using Association Rules].
[So] Source:Zhongguo Zhong Xi Yi Jie He Za Zhi;36(4):425-9, 2016 Apr.
[Is] ISSN:1003-5370
[Cp] Country of publication:China
[La] Language:chi
[Ab] Abstract:OBJECTIVE: To analyze the effect of Chinese herbs used by Prof. LI Tao on peripheral blood T subsets in treating multiple sclerosis (MS) by using association rules and statistical methods, thereby providing evidence for optimizing prescriptions. METHODS: Data of MS inpatients and outpatients recorded by data collecting system, Xiyuan Hospital, China Academy of Chinese Medical Sciences were resorted. The relationship between Chinese herbs and T cell subsets were analyzed using SPSS17.0 and Aprior module in SPSS Clementine 12.0. RESULTS: Radix bupleuri, Radix Paeoniae alba, Fructus Aurantii, Atractylodes, and Radix Glycyrrhizae were most commonly used herbal combinations.Radix Aconiti lateralis preparata and Rhizoma Smilacis glabrae were often added. Radix Aconiti lateralis preparata was associated with decreased Th1 cells (confidence level 83.78%, supportive level 36.26%). Decreased Th1 cell was associated with Radix Aconiti lateralis preparata (confidence level 71.26%, supportive level 36.26%).Radix Aconiti lateralis preparata was obviously associated with decreased Th1 cells. Radix Bupleuri, Radix Paeoniae alba, bitter orange, Atractylodes , Radix glycyrrhizae, and Radix Aconiti lateralis preparata could reduce peripheral blood Th1 subsets of MS patients and elevate Th2 subsets (all P < 0.01). CONCLUSIONS: The herbal combination of Radix Bupleuri, Radix Paeoniae alba, Fructus Aurantii, Atractylodes, Radix Glycyrrhizae, Rhizoma Smilacis glabrae, and Radix Aconiti lateralis preparata could lower peripheral blood Th1 cells and elevate Th2 cells, and prevent the relapse of MS possibly by reducing Th1 cells and elevating Th2 cells. Especially Radix Aconiti lateralis preparata played important roles in aforesaid changes of Th1 and Th2.
[Mh] MeSH terms primary: Drugs, Chinese Herbal/therapeutic use
Multiple Sclerosis/therapy
T-Lymphocyte Subsets/drug effects
[Mh] MeSH terms secundary: Aconitum/chemistry
Atractylodes/chemistry
Bupleurum/chemistry
China
Fruit/chemistry
Glycyrrhiza/chemistry
Humans
Paeonia/chemistry
Plant Roots/chemistry
Rhizome/chemistry
Smilacaceae/chemistry
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Nm] Name of substance:0 (Drugs, Chinese Herbal)
[Em] Entry month:1609
[Cu] Class update date: 161018
[Lr] Last revision date:161018
[Js] Journal subset:IM
[Da] Date of entry for processing:160622
[St] Status:MEDLINE

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[PMID]: 27251500
[Au] Autor:Lincha VR; Zhao BT; Woo MH; Yang IJ; Shin HM
[Ad] Address:Department of Physiology, College of Korean Medicine, Dongguk University.
[Ti] Title:Effects of Constituent Compounds of Smilax china on Nicotine-Induced Endothelial Dysfunction in Human Umbilical Vein Endothelial Cells.
[So] Source:Biol Pharm Bull;39(6):984-92, 2016.
[Is] ISSN:1347-5215
[Cp] Country of publication:Japan
[La] Language:eng
[Ab] Abstract:This study investigated the effects of compounds isolated from 70% ethanol (EtOH) extraction of Smilax china L. (SCE), a plant belonging to the family Smilacaceae on nicotine-induced endothelial dysfunction (ED) in human umbilical vein endothelial cells. We isolated 10 compounds from ethyl acetate (EtOAc) fraction of 70% EtOH extract of SCE and investigated their inhibitory effect on nicotine-induced ED in endothelial cells. Kaempferol, kaempferol 7-O-α-L-rhamnopyranoside, puerarin and ferulic acid showed strong inhibition of nicotine-induced vascular cell adhesion molecule (VCAM-1) expression while kaempferol, kaempferin, and caffeic acid attenuated intercellular adhesion molecule (ICAM-1) expression. Lepidoside, caffeic acid and methylsuccinic acid caused the highest up-regulated expression of endothelial nitric oxide synthase at the protein level with caffeic acid and ferulic acid showing strong inhibitory effects on inducible nitric oxide synthase (iNOS) expression. In addition, ferulic acid and kaempferol showed inhibition against interleukin-8 (IL-8) and interleukin-1ß (IL-1ß) expression while ferulic acid and caffeic acid showed comparatively higher inhibition of ED associated tumor necrosis factor-α (TNF-α) expression. These results show the potential of the aforementioned compounds to reverse the toxic effects of nicotine on the endothelium.
[Mh] MeSH terms primary: Human Umbilical Vein Endothelial Cells/drug effects
Nicotine/toxicity
Plant Extracts/pharmacology
Smilax
[Mh] MeSH terms secundary: Cell Survival/drug effects
Cells, Cultured
Cytokines/metabolism
Human Umbilical Vein Endothelial Cells/metabolism
Humans
Intercellular Adhesion Molecule-1/metabolism
Nitric Oxide/metabolism
Nitric Oxide Synthase Type II/metabolism
Nitric Oxide Synthase Type III/metabolism
Plant Leaves
Vascular Cell Adhesion Molecule-1/metabolism
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Cytokines); 0 (Plant Extracts); 0 (Vascular Cell Adhesion Molecule-1); 126547-89-5 (Intercellular Adhesion Molecule-1); 31C4KY9ESH (Nitric Oxide); 6M3C89ZY6R (Nicotine); EC 1.14.13.39 (NOS2 protein, human); EC 1.14.13.39 (NOS3 protein, human); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.13.39 (Nitric Oxide Synthase Type III)
[Em] Entry month:1701
[Cu] Class update date: 170131
[Lr] Last revision date:170131
[Js] Journal subset:IM
[Da] Date of entry for processing:160603
[St] Status:MEDLINE
[do] DOI:10.1248/bpb.b15-00997


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