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  1 / 1937 MEDLINE  
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[PMID]: 29244862
[Au] Autor:Mumba C; Skjerve E; Rich M; Rich KM
[Ad] Address:The University of Zambia, School of Veterinary Medicine, Department of Disease Control, Lusaka, Zambia.
[Ti] Title:Application of system dynamics and participatory spatial group model building in animal health: A case study of East Coast Fever interventions in Lundazi and Monze districts of Zambia.
[So] Source:PLoS One;12(12):e0189878, 2017.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:East Coast Fever (ECF) is the most economically important production disease among traditional beef cattle farmers in Zambia. Despite the disease control efforts by the government, donors, and farmers, ECF cases are increasing. Why does ECF oscillate over time? Can alternative approaches such as systems thinking contribute solutions to the complex ECF problem, avoid unintended consequences, and achieve sustainable results? To answer these research questions and inform the design and implementation of ECF interventions, we qualitatively investigated the influence of dynamic socio-economic, cultural, and ecological factors. We used system dynamics modelling to specify these dynamics qualitatively, and an innovative participatory framework called spatial group model building (SGMB). SGMB uses participatory geographical information system (GIS) concepts and techniques to capture the role of spatial phenomenon in the context of complex systems, allowing stakeholders to identify spatial phenomenon directly on physical maps and integrate such information in model development. Our SGMB process convened focus groups of beef value chain stakeholders in two distinct production systems. The focus groups helped to jointly construct a series of interrelated system dynamics models that described ECF in a broader systems context. Thus, a complementary objective of this study was to demonstrate the applicability of system dynamics modelling and SGMB in animal health. The SGMB process revealed policy leverage points in the beef cattle value chain that could be targeted to improve ECF control. For example, policies that develop sustainable and stable cattle markets and improve household income availability may have positive feedback effects on investment in animal health. The results obtained from a SGMB process also demonstrated that a "one-size-fits-all" approach may not be equally effective in policing ECF in different agro-ecological zones due to the complex interactions of socio-ecological context with important, and often ignored, spatial patterns.
[Mh] MeSH terms primary: Animal Husbandry
Theileriasis/virology
[Mh] MeSH terms secundary: Animals
Antibodies, Protozoan/administration & dosage
Cattle
Geographic Information Systems
Immunization
Theileriasis/epidemiology
Theileriasis/physiopathology
Zambia
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antibodies, Protozoan)
[Em] Entry month:1801
[Cu] Class update date: 180102
[Lr] Last revision date:180102
[Js] Journal subset:IM
[Da] Date of entry for processing:171216
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189878

  2 / 1937 MEDLINE  
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[PMID]: 28865228
[Au] Autor:Gunes V; Onmaz AC; Keles I; Varol K; Ekinci G
[Ad] Address:.
[Ti] Title:The diagnostic importance of coagulation parameters in cattle having natural theileriosis.
[So] Source:Pol J Vet Sci;20(2):369-376, 2017 Mar 01.
[Is] ISSN:1505-1773
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:The purpose of this study was to determine the diagnostic importance of coagulation parameters in cattle with natural theileriosis. Nine Holstein cross-breed cattle with theileriosis as infected group and 6 healthy Holstein cattle as control group were used in the present study. Mean fibrinogen level, thrombin time (TT), activated partial thromboplastin time (aPTT) and prothrombin time (PT) were not statistically different when control and infected groups compared, except for the D-dimer concentration. Quantitative D-dimer concentrations were determined by immune-turbidimetric assay. D-dimer values increased significantly (p<0.05) in infected group (631.55 ± 74.41 µg/L) compared to control group (370.00 ± 59.94 µg/L). D-dimer sensitivity and specificity were also determined at cut-off concentrations (372 µg/L). Sensitivity and specificity of D-dimer values were determined to be 88.89% and 83.33%, respectively. D-dimer is thought to be important indicator in the evaluation of the prognosis in theileriosis cases. Analysis of D-dimer values before and after treatment in controlled case studies were suggested in future studies to enlighten the issue.
[Mh] MeSH terms primary: Disseminated Intravascular Coagulation/veterinary
Theileriasis/blood
[Mh] MeSH terms secundary: Animals
Cattle
Disseminated Intravascular Coagulation/etiology
Female
Male
Theileriasis/complications
Theileriasis/pathology
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1711
[Cu] Class update date: 171106
[Lr] Last revision date:171106
[Js] Journal subset:IM
[Da] Date of entry for processing:170903
[St] Status:MEDLINE

  3 / 1937 MEDLINE  
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[PMID]: 28605963
[Au] Autor:Davitkov D; Davitkov D; Vucicevic M; Stanisic L; Radakovic M; Glavinic U; Stanimirovic Z
[Ad] Address:Department of Biology, Faculty of Veterinary Medicine, University of Belgrade , Belgrade , Serbia.
[Ti] Title:A molecular and haematological study of Theileria equi in Balkan donkeys.
[So] Source:Acta Vet Hung;65(2):234-241, 2017 06.
[Is] ISSN:0236-6290
[Cp] Country of publication:Hungary
[La] Language:eng
[Ab] Abstract:Equine piroplasmosis in donkeys has been recognised as a serious problem of major economic importance. The present molecular study is the first investigation of the presence of Theileria equi and Babesia caballi in Balkan donkeys and of the possible haematological alterations related to it. A total of 70 apparently healthy donkeys from Serbia were included in this study. The overall prevalence of T. equi infection in donkeys tested with multiplex PCR was 50%. There was no B. caballi-positive sample. Infections in donkeys included in this study seem to be associated with decreased red blood cell count, haemoglobin concentration, haematocrit and platelet count, and with increased white blood cell count, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. Altered haematological parameters in donkeys can lead to a decrease in working capacity and production performance. Further molecular research and long-term monitoring of equine piroplasmosis is needed in Serbia and throughout Europe.
[Mh] MeSH terms primary: Equidae/parasitology
Theileria/classification
Theileriasis/parasitology
[Mh] MeSH terms secundary: Animals
Babesiosis/epidemiology
Babesiosis/parasitology
Equidae/blood
Serbia/epidemiology
Seroepidemiologic Studies
Theileria/isolation & purification
Theileriasis/blood
Theileriasis/epidemiology
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1711
[Cu] Class update date: 171103
[Lr] Last revision date:171103
[Js] Journal subset:IM
[Da] Date of entry for processing:170614
[St] Status:MEDLINE
[do] DOI:10.1556/004.2017.023

  4 / 1937 MEDLINE  
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[PMID]: 28555954
[Au] Autor:Swilks E; Jenkins C; Poynting A; Collins D; Krebs GL
[Ad] Address:School of Animal and Veterinary Sciences, Charles Sturt University, Locked Bag 588, Wagga Wagga, New South Wales 2678, Australia.
[Ti] Title:Prevalence and effect of Theileria orientalis infection in homebred calves in the Gloucester region of New South Wales, Australia.
[So] Source:Aust Vet J;95(6):211-216, 2017 Jun.
[Is] ISSN:1751-0813
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:OBJECTIVES: To examine the prevalence of Theileria orientalis in the Gloucester area of New South Wales and its effect on individual animals. METHODS: Blood samples (EDTA and clotted blood) were collected from a total of 55 calves and their dams from 6 properties over a 16-week period. A total of 202 and 190 blood samples were collected from the calves and dams, respectively, and were examined via blood film for the presence of intraerythrocytic T. orientalis piroplasms. Packed cell volume (PCV) was measured to determine infection resulting in anaemia. The presence of antibodies against the T. orientalis major piroplasm surface protein (MPSP) was tested using ELISA. RESULTS: The overall prevalence of T. orientalis infection in both dams and calves from all herds examined was 95%. Mean peak parasitaemia was observed in calves between 6 and 9 weeks of age (P = 0.051), coinciding with a decline in mean PCV. Only 3 (6%) of the blood samples collected from the dams were positive for Theileria-associated antibodies and no significant relationship (P > 0.05) was found between the presence of antibodies in the dams and PCV levels in the calves. There was no evidence that passive transfer of antibodies from dams to calves protected the calves against a decline in PCV. CONCLUSION: This study confirmed a high prevalence of low-level Theileria infection, but low MPSP seroconversion rates, in dams and calves in an area where the disease has been endemic for a number of years.
[Mh] MeSH terms primary: Cattle Diseases/epidemiology
Cattle Diseases/parasitology
Theileriasis/epidemiology
[Mh] MeSH terms secundary: Anemia/epidemiology
Anemia/parasitology
Anemia/veterinary
Animals
Antibodies, Protozoan/blood
Cattle
Cattle Diseases/blood
Enzyme-Linked Immunosorbent Assay/veterinary
Female
Male
Merozoites
New South Wales/epidemiology
Polymerase Chain Reaction/veterinary
Prevalence
Theileria/genetics
Theileria/immunology
Theileriasis/blood
Theileriasis/complications
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antibodies, Protozoan)
[Em] Entry month:1710
[Cu] Class update date: 171006
[Lr] Last revision date:171006
[Js] Journal subset:IM
[Da] Date of entry for processing:170531
[St] Status:MEDLINE
[do] DOI:10.1111/avj.12593

  5 / 1937 MEDLINE  
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[PMID]: 28495200
[Au] Autor:Ali AM; Salih DA; Njahira MN; Hassan SK; El Hussein AM; Liu Z; Yin H; Pelle R; Skilton RA
[Ad] Address:Biosciences eastern and central Africa-International Livestock Research Institute (BecA-ILRI) Hub, P.O. Box 30709-00100, Nairobi, Kenya; University of Khartoum, Faculty of Veterinary Medicine, Khartoum, Sudan. Electronic address: Awadia_mosa@hotmail.com.
[Ti] Title:Genotyping of Theileria lestoquardi from sheep and goats in Sudan to support control of Malignant Ovine Theileriosis.
[So] Source:Vet Parasitol;239:7-14, 2017 May 30.
[Is] ISSN:1873-2550
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Theileriosis, caused by parasitic protozoa of the genus Theileria parasites, are among the major tick-borne diseases of ruminant livestock. The largest economic losses are attributed in particular to those caused by the leukoproliferative species of Theileria: T. parva, T. annulata and T. lestoquardi. Theileria lestoquardi is transmitted by Hyalomma ticks and causes malignant ovine theileriosis (MOT), a disease that is particularly prevalent in Sudan. The disease is considered of a high economic importance in Sudan, where export of sheep is a major component of the national economy. A live vaccine based on a Sudanese isolate of T. lestoquardi (Atbara strain) was previously developed for the control of MOT in Sudan, but not yet deployed in the field. The present study aims to genetically characterize and compare samples of T. lestoquardi circulating in Sudan as well as the live vaccine isolate in order to understand vaccine breakthroughs and failure that may occur. Sheep and goats blood samples were collected from six regions in Sudan that are known to be endemic for T. lestoquardi infection or have experienced outbreaks of MOT. Blood samples infected with T. lestoquardi were identified by PCR or RLB. Genotyping was carried out by (1) sequencing the homologues of two T. parva CD8 T cell antigen genes, Tp1 and Tp2, and (2) using a panel of seven micro- and mini-satellite markers. A total of 100 T. lestoquardi positive field samples and the T. lestoquardi (Atbara) vaccine were genotyped. The results showed that all samples had mixed genotypes, with several alleles identified at one or more loci. The gene diversity ranged from 0.7840 (TS8) to 0.2133 (TS12) with mean values of 0.5470. PCA revealed three clusters of the parasite in Sudan; interestingly one independent cluster was clearly seen, corresponding to the vaccine isolate. The T. lestoquardi Tp1 homologue showed higher homology with T. annulata than with T. parva sequences included the defined single CD8 T cell target epitope region. The result indicates that multiple genotypes are a common feature of T. lestoquardi infection in Sudan. Both genotyping and the sequencing results clearly showed that the vaccine isolate is highly distinct from the field samples. This finding raised the question whether vaccination with the prepared lived vaccine will effectively protect animals against challenges by the field isolates of T. lestoquardi. The results of this work will inform on the best approach for controlling MOT in Sudan.
[Mh] MeSH terms primary: Goat Diseases/parasitology
Protozoan Vaccines
Sheep Diseases/parasitology
Theileria/genetics
Theileriasis/parasitology
[Mh] MeSH terms secundary: Amino Acid Sequence
Animals
Gene Expression Regulation/physiology
Genotyping Techniques/methods
Genotyping Techniques/veterinary
Goat Diseases/epidemiology
Goat Diseases/prevention & control
Goats
Protozoan Proteins/genetics
Protozoan Proteins/metabolism
Sheep
Sheep Diseases/epidemiology
Sheep Diseases/prevention & control
Sudan/epidemiology
Theileria/classification
Theileria/immunology
Theileriasis/epidemiology
Theileriasis/prevention & control
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Protozoan Proteins); 0 (Protozoan Vaccines)
[Em] Entry month:1710
[Cu] Class update date: 171031
[Lr] Last revision date:171031
[Js] Journal subset:IM
[Da] Date of entry for processing:170513
[St] Status:MEDLINE

  6 / 1937 MEDLINE  
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[PMID]: 28358861
[Au] Autor:Kolte SW; Larcombe SD; Jadhao SG; Magar SP; Warthi G; Kurkure NV; Glass EJ; Shiels BR
[Ad] Address:Nagpur Veterinary College, Maharashtra Animal and Fishery Sciences, Nagpur, Maharashtra, India.
[Ti] Title:PCR diagnosis of tick-borne pathogens in Maharashtra state, India indicates fitness cost associated with carrier infections is greater for crossbreed than native cattle breeds.
[So] Source:PLoS One;12(3):e0174595, 2017.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Tick-borne pathogens (TBP) are responsible for significant economic losses to cattle production, globally. This is particularly true in countries like India where TBP constrain rearing of high yielding Bos taurus, as they show susceptibility to acute tick borne disease (TBD), most notably tropical theileriosis caused by Theileria annulata. This has led to a programme of cross breeding Bos taurus (Holstein-Friesian or Jersey) with native Bos indicus (numerous) breeds to generate cattle that are more resistant to disease. However, the cost to fitness of subclinical carrier infection in crossbreeds relative to native breeds is unknown, but could represent a significant hidden economic cost. In this study, a total of 1052 bovine blood samples, together with associated data on host type, sex and body score, were collected from apparently healthy animals in four different agro-climatic zones of Maharashtra state. Samples were screened by PCR for detection of five major TBPs: T. annulata, T. orientalis, B. bigemina, B. bovis and Anaplasma spp.. The results demonstrated that single and co-infection with TBP are common, and although differences in pathogen spp. prevalence across the climatic zones were detected, simplistic regression models predicted that host type, sex and location are all likely to impact on prevalence of TBP. In order to remove issues with autocorrelation between variables, a subset of the dataset was modelled to assess any impact of TBP infection on body score of crossbreed versus native breed cattle (breed type). The model showed significant association between infection with TBP (particularly apicomplexan parasites) and poorer body condition for crossbreed animals. These findings indicate potential cost of TBP carrier infection on crossbreed productivity. Thus, there is a case for development of strategies for targeted breeding to combine productivity traits with disease resistance, or to prevent transmission of TBP in India for economic benefit.
[Mh] MeSH terms primary: Cattle Diseases/diagnosis
Theileria annulata/isolation & purification
Theileriasis/genetics
Tick-Borne Diseases/diagnosis
[Mh] MeSH terms secundary: Animals
Cattle
Cattle Diseases/genetics
Cattle Diseases/parasitology
India
Polymerase Chain Reaction/methods
Theileria annulata/genetics
Theileria annulata/pathogenicity
Theileriasis/diagnosis
Theileriasis/parasitology
Tick-Borne Diseases/genetics
Tick-Borne Diseases/veterinary
Ticks/parasitology
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1708
[Cu] Class update date: 170828
[Lr] Last revision date:170828
[Js] Journal subset:IM
[Da] Date of entry for processing:170331
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0174595

  7 / 1937 MEDLINE  
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[PMID]: 28332070
[Au] Autor:Laisser EL; Chenyambuga SW; Karimuribo ED; Msalya G; Kipanyula MJ; Mwilawa AJ; Mdegela RH; Kusiluka LJ
[Ad] Address:School Quality Assurance Department, Ministry of Education, Science and Technology, Eastern Zone, P.O. Box 325, Morogoro, Tanzania. emalaisser@gmail.com.
[Ti] Title:A review on prevalence, control measure, and tolerance of Tanzania Shorthorn Zebu cattle to East Coast fever in Tanzania.
[So] Source:Trop Anim Health Prod;49(4):813-822, 2017 Apr.
[Is] ISSN:1573-7438
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:In Tanzania, control of East Coast fever (ECF) has predominantly relied on tick control using acaricides and chemotherapy, little on ECF vaccination, and very little on dissemination regarding animal immunization. In this paper, the prevalence, control measure, and tolerance of Tanzania Shorthorn Zebu (TSHZ) cattle to ECF are reviewed. In addition, the opportunities available for reducing the use of acaricides for the benefit of the farmers in terms of reduction of costs of purchasing acaricides and environmental pollution are described. The tick distribution and epidemiological factors for ECF such as the agro-ecological zones (AEZ), livestock production systems (LPS), strain, and age of the animals are also described. These factors influence the epidemiology of ECF and the distribution of TSHZ strains in different geographic locations of Tanzania. We have further showed that there is a tendency of farmers to select among the strains of TSHZ for animals which can tolerate ticks and ECF and crossbreed them with their local strains with the aim of benefiting from the inherent characteristics of the most tolerant strains. Generally, many strains of TSHZ cattle are tolerant to tick infestation and ECF infection and can be bred to respond to the needs of the people. In this review paper, we recommend that in future, ECF epidemiological studies should account for factors such as livestock production system, agro-climate, breed of animal, tick control strategy, and the dynamic interactions between them. In conclusion, we have demonstrated that an integrated control method involving use of acaricides, immunization, and ECF-tolerant/-resistant animals is required.
[Mh] MeSH terms primary: Theileriasis/epidemiology
Tick-Borne Diseases/veterinary
[Mh] MeSH terms secundary: Acaricides
Animals
Cattle
Immunization/veterinary
Livestock
Prevalence
Tanzania/epidemiology
Theileriasis/immunology
Theileriasis/prevention & control
Tick Control
Tick Infestations/immunology
Tick Infestations/prevention & control
Tick Infestations/veterinary
Tick-Borne Diseases/epidemiology
Tick-Borne Diseases/immunology
Tick-Borne Diseases/prevention & control
Ticks
Vaccination
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Nm] Name of substance:0 (Acaricides)
[Em] Entry month:1706
[Cu] Class update date: 171102
[Lr] Last revision date:171102
[Js] Journal subset:IM
[Da] Date of entry for processing:170324
[St] Status:MEDLINE
[do] DOI:10.1007/s11250-017-1266-z

  8 / 1937 MEDLINE  
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[PMID]: 28288762
[Au] Autor:Zanet S; Bassano M; Trisciuoglio A; Taricco I; Ferroglio E
[Ad] Address:University of Turin, Dept. of Veterinary Sciences, Largo Braccini 2, 10095 Grugliasco, To, Italy.
[Ti] Title:Horses infected by Piroplasms different from Babesia caballi and Theileria equi: species identification and risk factors analysis in Italy.
[So] Source:Vet Parasitol;236:38-41, 2017 Mar 15.
[Is] ISSN:1873-2550
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Equine Piroplasmosis (EP) caused by Theileria equi and Babesia caballi is a disease affecting the health and the international movement of horses. In order to assess prevalence of Piroplasmid infection in the Northwestern part of Italy and to evaluate the associated risk factors, whole blood was collected from 135 horses from 7 different stables across the study area. PCR and sequencing were used to assess prevalence of infection and to identify detected Piroplasms to species level. A total of 23 horses (P=17.04%; CI95%: 10.70-23.38%) was found to be infected with Piroplasms and T. equi was the most prevalent species, found in 18 animals (P=13.33%; CI95%: 7.60%-19.07%). Although B. caballi was never detected, the presence of parasites belonging to the genus Babesia was confirmed by sequencing in 5 horses, 3 of which were infected with B. canis (P=2.22%; CI95% 0.76%-6.33%), and 2 with B. capreoli (P=1.48%; CI95% 0.41%-5.24%). The natural reservoir hosts of B. canis and B. capreoli are the domestic dog and roe deer Capreolus capreolus respectively. These findings pose attention to the need of considering in future epidemiological and clinical studies, other Apicomplexan species as able to infect horses.
[Mh] MeSH terms primary: Babesia/isolation & purification
Babesiosis/epidemiology
Horse Diseases/epidemiology
Horse Diseases/parasitology
Theileria/isolation & purification
Theileriasis/epidemiology
[Mh] MeSH terms secundary: Animals
Babesia/classification
Babesia/genetics
Babesiosis/parasitology
DNA, Protozoan/genetics
Female
Horses
Italy/epidemiology
Male
Phylogeny
Prevalence
RNA, Ribosomal, 18S/genetics
Risk Factors
Sequence Analysis, DNA/veterinary
Theileria/classification
Theileria/genetics
Theileriasis/parasitology
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Protozoan); 0 (RNA, Ribosomal, 18S)
[Em] Entry month:1709
[Cu] Class update date: 170908
[Lr] Last revision date:170908
[Js] Journal subset:IM
[Da] Date of entry for processing:170315
[St] Status:MEDLINE

  9 / 1937 MEDLINE  
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[PMID]: 28286324
[Au] Autor:Kamyingkird K; Cao S; Tuvshintulga B; Salama A; Mousa AA; Efstratiou A; Nishikawa Y; Yokoyama N; Igarashi I; Xuan X
[Ad] Address:National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan; Department of Parasitology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok, 10900, Thailand.
[Ti] Title:Effects of dihydroorotate dehydrogenase (DHODH) inhibitors on the growth of Theileria equi and Babesia caballi in vitro.
[So] Source:Exp Parasitol;176:59-65, 2017 May.
[Is] ISSN:1090-2449
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Theileria equi and Babesia caballi are the causative agents of equine piroplasmosis (EP), which affects equine production in various parts of the world. However, a safe and effective drug is not currently available for treatment of EP. Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine synthesis pathway and has been known as a novel drug target for several apicomplexan protozoan parasites. In this study, we evaluated four DHODH inhibitors; atovaquone (ATV), leflunomide (LFN), brequinar (Breq), and 7-hydroxy-5-[1,2,4] triazolo [1,5,a] pyrimidine (TAZ) on the growth of T. equi and B. caballi in vitro and compared them to diminacene aceturate (Di) as the control drug. The growth of T. equi and B. caballi was significantly hindered by all inhibitors except TAZ. The half maximal inhibitory concentration (IC ) of ATV, LFN, Breq and Di against T. equi was approximately 0.028, 109, 11 and 40 µM, respectively, whereas the IC of ATV, LFN, Breq and Di against B. caballi was approximately 0.128, 193, 5.2 and 16.2 µM, respectively. Using bioinformatics and Western blot analysis, we showed that TeDHODH was similar to other Babesia parasite DHODHs, and confirmed that targeting DHODHs could be useful for the development of novel chemotherapeutics for treatment of EP.
[Mh] MeSH terms primary: Babesia/drug effects
Enzyme Inhibitors/pharmacology
Oxidoreductases Acting on CH-CH Group Donors/antagonists & inhibitors
Theileria/drug effects
[Mh] MeSH terms secundary: Amino Acid Sequence
Animals
Antiprotozoal Agents/pharmacology
Atovaquone/pharmacology
Babesia/classification
Babesia/growth & development
Babesiosis/drug therapy
Babesiosis/parasitology
Biphenyl Compounds/pharmacology
Computational Biology
Diminazene/analogs & derivatives
Diminazene/pharmacology
Enzyme Inhibitors/therapeutic use
Horse Diseases/drug therapy
Horse Diseases/parasitology
Horses
Inhibitory Concentration 50
Isoxazoles/pharmacology
Mice
Molecular Weight
Oxidoreductases Acting on CH-CH Group Donors/chemistry
Phylogeny
Plasmodium berghei/drug effects
Plasmodium berghei/growth & development
Pyrimidines/pharmacology
Pyrimidines/therapeutic use
Theileria/classification
Theileria/growth & development
Theileriasis/drug therapy
Theileriasis/parasitology
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antiprotozoal Agents); 0 (Biphenyl Compounds); 0 (Enzyme Inhibitors); 0 (Isoxazoles); 0 (Pyrimidines); 5XL19F49H6 (brequinar); EC 1.3.- (Oxidoreductases Acting on CH-CH Group Donors); EC 1.3.5.2 (dihydroorotate dehydrogenase); G162GK9U4W (leflunomide); JI8SAD85NO (diminazene aceturate); K8CXK5Q32L (pyrimidine); Y5G36EEA5Z (Diminazene); Y883P1Z2LT (Atovaquone)
[Em] Entry month:1704
[Cu] Class update date: 170411
[Lr] Last revision date:170411
[Js] Journal subset:IM
[Da] Date of entry for processing:170314
[St] Status:MEDLINE

  10 / 1937 MEDLINE  
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[PMID]: 28249769
[Au] Autor:Yin F; Liu J; Liu A; Li Y; Luo J; Guan G; Yin H
[Ad] Address:State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Xujiaping 1, Lanzhou, Gansu 730046, PR China.
[Ti] Title:Rapid diagnosis of Theileria annulata by recombinase polymerase amplification combined with a lateral flow strip (LF-RPA) in epidemic regions.
[So] Source:Vet Parasitol;237:125-129, 2017 Apr 15.
[Is] ISSN:1873-2550
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Rapid and accurate diagnosis of Theileria annulata infection contributes to the formulation of strategies to eradicate this parasite. A simple and efficient diagnostic tool, recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip, was used in detection of Theileria and compared to other methods that require expensive instruments and skilled personnel. Herein, we established and optimized an LF-RPA method to detect the cytochrome b gene of T. annulata mitochondrial DNA from experimentally infected and field-collected blood samples. This method has many unparalleled characteristics, including that it is rapid (clear detection in 5min at constant temperature), sensitive (the limitation of detection is at least 2pg genomic DNA), and specific (no cross-reaction with other piroplasms that infect cattle). The LF-RPA assay was evaluated via testing 17 field blood samples and comparing the results of that of a PCR, showing 100% agreement, which demonstrates the ability of the LF-RPA assay to detect T. annulata infections in small number of samples (n=17). Taken together, the results indicate that this method could be used as an ideal diagnostic tool for detecting T. annulata in endemic regions with limited to fewer and local resources and could also be a potential technique for the surveillance and control of blood protozoa.
[Mh] MeSH terms primary: Epidemics/veterinary
Theileria annulata/isolation & purification
Theileriasis/diagnosis
[Mh] MeSH terms secundary: Animals
Cattle
Cytochromes b/genetics
DNA, Mitochondrial/genetics
Polymerase Chain Reaction/veterinary
Recombinases/metabolism
Theileria annulata/genetics
Theileriasis/epidemiology
Theileriasis/parasitology
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Mitochondrial); 0 (Recombinases); 9035-37-4 (Cytochromes b)
[Em] Entry month:1709
[Cu] Class update date: 170926
[Lr] Last revision date:170926
[Js] Journal subset:IM
[Da] Date of entry for processing:170303
[St] Status:MEDLINE


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