Database : MEDLINE
Search on : Thromboxane and B2 [Words]
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[PMID]: 29346441
[Au] Autor:Gifford SC; Strachan BC; Xia H; Vörös E; Torabian K; Tomasino TA; Griffin GD; Lichtiger B; Aung FM; Shevkoplyas SS
[Ad] Address:Halcyon Biomedical Incorporated, Friendswood, Texas, United States of America.
[Ti] Title:A portable system for processing donated whole blood into high quality components without centrifugation.
[So] Source:PLoS One;13(1):e0190827, 2018.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: The use of centrifugation-based approaches for processing donated blood into components is routine in the industrialized world, as disparate storage conditions require the rapid separation of 'whole blood' into distinct red blood cell (RBC), platelet, and plasma products. However, the logistical complications and potential cellular damage associated with centrifugation/apheresis manufacturing of blood products are well documented. The objective of this study was to evaluate a proof-of-concept system for whole blood processing, which does not employ electromechanical parts, is easily portable, and can be operated immediately after donation with minimal human labor. METHODS AND FINDINGS: In a split-unit study (n = 6), full (~500mL) units of freshly-donated whole blood were divided, with one half processed by conventional centrifugation techniques and the other with the new blood separation system. Each of these processes took 2-3 hours to complete and were performed in parallel. Blood products generated by the two approaches were compared using an extensive panel of cellular and plasma quality metrics. Comparison of nearly all RBC parameters showed no significant differences between the two approaches, although the portable system generated RBC units with a slight but statistically significant improvement in 2,3-diphosphoglyceric acid concentration (p < 0.05). More notably, several markers of platelet damage were significantly and meaningfully higher in products generated with conventional centrifugation: the increase in platelet activation (assessed via P-selectin expression in platelets before and after blood processing) was nearly 4-fold higher for platelet units produced via centrifugation, and the release of pro-inflammatory mediators (soluble CD40-ligand, thromboxane B2) was significantly higher for centrifuged platelets as well (p < 0.01). CONCLUSION: This study demonstrated that a simple, passive system for separating donated blood into components may be a viable alternative to centrifugation-particularly for applications in remote or resource-limited settings, or for patients requiring highly functional platelet product.
[Mh] MeSH terms primary: Blood Donors
Blood
Specimen Handling
[Mh] MeSH terms secundary: Centrifugation
Humans
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Entry month:1802
[Cu] Class update date: 180221
[Lr] Last revision date:180221
[Js] Journal subset:IM
[Da] Date of entry for processing:180119
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190827

  2 / 9429 MEDLINE  
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[PMID]: 29439623
[Au] Autor:Zhang C; Zhu Y; Shen Y; Zuo C
[Ad] Address:1 Department of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China.
[Ti] Title:Aspirin Ameliorates Preeclampsia Induced by a Peroxisome Proliferator-Activated Receptor Antagonist.
[So] Source:Reprod Sci;:1933719118756746, 2018 Jan 01.
[Is] ISSN:1933-7205
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Reduced expression of peroxisome proliferator-activated receptor γ (PPARγ) in the placenta was found in women with severe preeclampsia. Aspirin is currently used as the only recommended intervention in pregnancies for prevention of preeclampsia. In this study, we aimed to investigate whether aspirin could attenuate PPARγ inhibitor (T0070907)-induced preeclampsia and its impact on expression of PPARγ. Sixty Sprague-Dawley rats were used and treated with different doses of aspirin (0, 1, and 1.5 mg/kg) in presence or absence of PPARγ antagonist, T0070907. We found that mean arterial blood pressure was significantly reduced by aspirin treatment in T0070907-exposed rats. T0070907 exposure also led to significant decrease in fetal weight and increase in placental weights. However, 1.5 mg/kg of aspirin reversed these effects of T0070907. Additionally, aspirin also reversed T0070907-induced changes in the levels of thromboxane B2, vascular endothelial growth factor, soluble fms-like tyrosine kinase, and matrix metalloproteinase 2 in both maternal blood and placental tissue. The increased messenger RNA and protein levels of Cox1 and Cox2 induced by T0070907 were markedly reduced by aspirin treatment. Importantly, T0070907 repressed both transcriptional and translational levels of PPARγ, which were reversed by aspirin. In conclusion, this study suggests that aspirin prevented the occurrence of preeclampsia, which is possibly through enhancing both transcriptional and translational levels of PPARγ.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180214
[Lr] Last revision date:180214
[St] Status:Publisher
[do] DOI:10.1177/1933719118756746

  3 / 9429 MEDLINE  
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[PMID]: 29437834
[Au] Autor:Colas RA; Souza PR; Walker ME; Burton M; Marques RM; Zaslona Z; Curtis AM; Dalli J
[Ad] Address:William Harvey Research Institute, Queen Mary University of London.
[Ti] Title:Impaired Production and Diurnal Regulation of Vascular RvD Increases Systemic Inflammation and Cardiovascular Disease.
[So] Source:Circ Res;, 2018 Feb 05.
[Is] ISSN:1524-4571
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Diurnal mechanisms are central to regulating host responses. Recent studies uncovered a novel family of mediators termed as specialized pro-resolving mediators (SPM) that terminate inflammation without interfering with the immune response. Little is known on their diurnal regulation. Herein we investigated the diurnal regulation of SPM in humans and their role in controlling peripheral blood leukocyte and platelet activation. Using lipid mediator profiling and healthy volunteers we found that plasma concentrations of n-3 docosapentaenoic acid-derived D-series resolvins (RvD ) were regulated in a diurnal manner. The production and diurnal regulation of these mediators was markedly altered in patients at risk of myocardial infarct. These changes were associated with decreased 5-lipoxygenase expression and activity as well as increased systemic adenosine concentrations. We also found a significant negative correlation between plasma RvD and markers of platelet, monocyte and neutrophil activation including CD63 and CD11b. Incubation of RvD with peripheral blood from healthy volunteers and patients with cardiovascular disease significantly and dose-dependently decreased platelet and leukocyte activation. Furthermore, administration of RvD5 to apolipoprotein E deficient mice significantly reduced platelet-leukocyte aggregates, vascular thromboxane B2 concentrations and aortic lesions. These results demonstrate that peripheral blood RvD are diurnally regulated in humans and dysregulation in the production of these mediators may lead to cardiovascular disease.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180213
[Lr] Last revision date:180213
[St] Status:Publisher

  4 / 9429 MEDLINE  
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[PMID]: 29301283
[Au] Autor:Lanocha-Arendarczyk N; Baranowska-Bosiacka I; Kot K; Gutowska I; Kolasa-Wolosiuk A; Chlubek D; Kosik-Bogacka D
[Ad] Address:Department of Biology and Medical Parasitology, Pomeranian Medical University in Szczecin, 70-204 Szczecin, Poland. nlanocha@pum.edu.pl.
[Ti] Title:Expression and Activity of COX-1 and COX-2 in Acanthamoeba sp.-Infected Lungs According to the Host Immunological Status.
[So] Source:Int J Mol Sci;19(1), 2018 Jan 02.
[Is] ISSN:1422-0067
[Cp] Country of publication:Switzerland
[La] Language:eng
[Ab] Abstract:Little is known about the pathomechanism of pulmonary infections caused by sp. Therefore, the aim of this study was to determine whether sp. may affect the expression and activity of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2), resulting in the altered levels of their main products, prostaglandins (PGE2) and thromboxane B2 (TXB2), in lungs of immunocompetent or immunosuppressed hosts. sp. induced a strong expression of COX-1 and COX-2 proteins in the lungs of immunocompetent mice, which, however, did not result in significant differences in the expression of PGE2 and TXB2. Our immunohistochemical analysis showed that immunosuppression induced by glucocorticoids in sp.-infected mice caused a decrease in COX-1 and COX-2 (not at the beginning of infection) in lung tissue. These results suggest that similar to COX-2, COX-1 is an important mediator of the pathophysiology in experimental pulmonary acanthamoebiasis. We suggest that the signaling pathways important for sp. induction of lung infection might interact with each other and depend on the host immune status.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180213
[Lr] Last revision date:180213
[St] Status:In-Process

  5 / 9429 MEDLINE  
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[PMID]: 29328426
[Au] Autor:Wu HC; Horng CT; Tsai SC; Lee YL; Hsu SC; Tsai YJ; Tsai FJ; Chiang JH; Kuo DH; Yang JS
[Ad] Address:Department of Nutrition, Kaohsiung Armed Forces General Hospital, Kaohsiung 80284, Taiwan, R.O.C.
[Ti] Title:Relaxant and vasoprotective effects of ginger extracts on porcine coronary arteries.
[So] Source:Int J Mol Med;41(4):2420-2428, 2018 Apr.
[Is] ISSN:1791-244X
[Cp] Country of publication:Greece
[La] Language:eng
[Ab] Abstract:Ginger (Zingiber officinale Roscoe) is a popular Chinese herbal medicine, which is considered to warm the stomach and dispel cold in traditional Chinese medicine. Ginger is widely used to treat stomach disorders, and it has been reported to exhibit antithrombotic activity via the inhibition of platelet aggregation and thromboxane B2 production in vitro. Cardiovascular disease is associated with the aberrant functioning of the heart and circulatory system; the relatively narrow vessels of the circulation are commonly affected and blocked by atherosclerosis, which may result in angina or heart attack. Numerous drugs and medicines are used to treat myocardial infarction; however, they are often associated with numerous side effects. Therefore, it is important to identify substitutive drugs with no unbearable side effects. In the present study, the relaxant effects of ginger crude extract (GCE) were determined on porcine coronary arteries. The DPPH radical scavenging assay, lucigenin­enhanced chemiluminescence assay and western blot analysis were used to individually detect antioxidant assay of ginger extraction or superoxide anion produced by endothelial cells and molecular signaling. The results indicated that GCE induced relaxation of porcine coronary arteries in an endothelium­dependent manner. GCE increased vasoprotection via the suppression of nitric oxide synthase and cyclooxygenase. In addition, GCE possessed antioxidant ability, as determined using 1,1­diphenyl­2­picrylhydrazyl and lucigenin­enhanced chemiluminescence assays. Taken together, the present study demonstrated that GCE exerts marked vasoprotective effects and free radical­scavenging activities in porcine coronary arteries.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180212
[Lr] Last revision date:180212
[St] Status:In-Process
[do] DOI:10.3892/ijmm.2018.3380

  6 / 9429 MEDLINE  
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[PMID]: 29362444
[Au] Autor:Savitz JB; Teague TK; Misaki M; Macaluso M; Wurfel BE; Meyer M; Drevets D; Yates W; Gleason O; Drevets WC; Preskorn SH
[Ad] Address:Laureate Institute for Brain Research, Tulsa, OK, USA. jsavitz@laureateinstitute.org.
[Ti] Title:Treatment of bipolar depression with minocycline and/or aspirin: an adaptive, 2×2 double-blind, randomized, placebo-controlled, phase IIA clinical trial.
[So] Source:Transl Psychiatry;8(1):27, 2018 Jan 24.
[Is] ISSN:2158-3188
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Given evidence of chronic inflammation in bipolar disorder (BD), we tested the efficacy of aspirin and minocycline as augmentation therapy for bipolar depression. Ninety-nine depressed outpatients with BD were enrolled in a 6 week, double-blind, placebo-controlled trial, and randomized to one of four groups: active minocycline (100 mg b.i.d.) + active aspirin (81 mg b.i.d.) (M + A); active minocycline + placebo aspirin (M + P); placebo-minocycline + active aspirin (A + P); and placebo-minocycline + placebo aspirin (P + P). A blinded interim analysis mid-way through the study led to the dropping of the M + P and A + P arms from further enrollment giving numbers per group who were included in the final analysis of: 30 (M + A), 18 (M + P), 19 (A + P), and 28 (P + P). When the study started, there were three primary outcome measures. Based on the results of the interim analysis, the primary outcome variable, response to treatment as defined by >50% decrease in Montgomery-Äsberg Depression Rating Scale (MADRS) score was maintained. The other two (i.e., the change in mean MADRS score from baseline to end of study and the remission rate, with remission being defined as a score of <11 on the MADRS) were reduced to exploratory outcome measures because the interim analysis indicated that the study was adequately powered to test differences in response rate but not the mean change in MADRS scores or remission rates. CRP and IL-6 were assayed to measure inflammation. Urinary thromboxane B2 (11-D-TXB ) concentrations, which were significantly increased at baseline in the combined BD sample (n = 90) vs. a healthy control group (n = 27), served as an indirect marker of cyclooxygenase (COX) activity. In a two-group analysis, the M + A group showed a greater response rate than the P + P group (p(one-tailed) = 0.034, OR = 2.93, NNT = 4.7). When all four arms were included in the analysis, there was a main effect of aspirin on treatment response that was driven by both the M + A and the A + P groups (p(two-tailed) = 0.019, OR = 3.67, NNT = 4.0). Additionally, there was a significant 3-way interaction between aspirin, minocycline, and IL-6, indicating that response to minocycline was significantly greater in participants in the M + P group with higher IL-6 concentrations. Further, participants in the M + P group who responded to treatment had significantly greater decreases in IL-6 levels between baseline and visit 7 vs. non-responders. Regarding the exploratory outcomes, there was a main effect for aspirin on the remission rate (χ = 4.14, p(2t) = 0.04, OR = 2.52, NNT = 8.0). There was no significant main effect of aspirin or minocycline on the mean change in MADRS score across visits. Aspirin and minocycline may be efficacious adjunctive treatments for bipolar depression. Given their potential import, additional studies to confirm and extend these findings are warranted.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180209
[Lr] Last revision date:180209
[St] Status:In-Data-Review
[do] DOI:10.1038/s41398-017-0073-7

  7 / 9429 MEDLINE  
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[PMID]: 29414763
[Au] Autor:Hamabata T; Nakamura T; Masuko S; Maeda S; Murata T
[Ad] Address:The University of Tokyo, Japan.
[Ti] Title:Production of lipid mediators across different disease stages of dextran sulfate sodium-induced colitis in mice.
[So] Source:J Lipid Res;, 2018 Feb 02.
[Is] ISSN:1539-7262
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Although several studies have revealed the role of different lipid mediators in colitis, the comprehensive analysis of their production across different phases of colitis remained unclear. We performed the above analysis in the dextran sulfate sodium (DSS)-induced colitis model using LC-MS/MS. Oral administration of 2% DSS in mice for 4 days resulted in severe intestinal inflammation by day 7, which gradually subsided by day 18. Based on the disease scoring index (assigned on the basis of fecal condition and weight loss), we defined the phases of colitis as induction (day 0-4), acute inflammation (day 4-7), recovery (day 7-9), and late recovery (day 9-18). Across all phases, 58 lipid mediators were detected in the inflamed colon tissue. In the induction phase, the production of n-6 fatty acid-derived prostaglandin E2 and thromboxane B2 increased by ~2-fold. In the acute inflammation phase, the production of n-6 fatty acid-derived leukotrienes increased by > 10-fold, while that of n-3 fatty acid-derived hydroxyeicosapentaenoic acids and dihydroxyeicosatetraenoic acids decreased. In the recovery phase, a precursor of protectin D1 (17-hydroxydocosahexaenoic acid) increased over 3-fold. These observations suggested dynamic changes in the production of lipid mediators across different phases of the disease and their potential regulation in healing colitis.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180207
[Lr] Last revision date:180207
[St] Status:Publisher

  8 / 9429 MEDLINE  
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[PMID]: 29357411
[Au] Autor:Lee H; Roshanravan H; Wang Y; Okamoto K; Ryu J; Shrivastav S; Qu P; Kopp JB; Kopp JB
[Ad] Address:Institute of Heart and Vessel Diseases, Affiliated Second Hospital of Dalian Medical University, China.
[Ti] Title:APOL1 renal risk variants induce aberrant THP-1 monocyte differentiation and increase eicosanoid production via enhanced expression of cyclooxygenase 2.
[So] Source:Am J Physiol Renal Physiol;, 2018 Jan 10.
[Is] ISSN:1522-1466
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Apolipoprotein L1 (APOL1) genetic variants are strongly associated with kidney disease. We investigated the role of APOL1 variants in monocyte differentiation and eicosanoid production in macrophages, as activated tissue macrophages in kidney might contribute to kidney injury. In human monocyte THP-1 cells, transient overexpression of APOL1 (G0, G1, G2) by transfection resulted in a 5 to 11-fold increase in CD14 and CD68 gene expression, similar to that seen with phorbol-12-myristate acetate treatment. All APOL1 variants caused monocytes to differentiate into atypical M1 macrophages with marked increase in M1 markers CD80, TNF, IL1B, and IL6 and modest increase in the M2 marker CD163 as compared to control cells. APOL1-G1 transfection induced additional CD206 and TGFB1 expression and APOL1-G2 transfection induced additional CD204 and TGFB1 expression. Gene expression of prostaglandin E2 (PGE2) synthase and thromboxane synthase and both gene and protein expression of cyclooxygenase-2 (COX2) were increased by APOL1-G1 and -G2 variants compared to -G0 transfection. Higher levels of PGE2 and thromboxane B2, a stable metabolite of thromboxane A2, and TGF-ß1 were released into the supernatant of cultured THP-1 cells transfected with APOL1-G1 and -G2, but not -G0. The increase in PGE2, thromboxane B2, and TGF-ß1 was inhibited by COX2-specific inhibitor CAY10404 but not by COX1-specific inhibitor SC-560. These results demonstrate a novel role of APOL1 variants in the regulation of monocyte differentiation and eicosanoid metabolism, which could modify the immune response and promote inflammatory signaling within the local targeted organs and tissues including the kidney.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180131
[Lr] Last revision date:180131
[St] Status:Publisher
[do] DOI:10.1152/ajprenal.00254.2017

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[PMID]: 29292766
[Au] Autor:Sikora M; Goschorska M; Baranowska-Bosiacka I; Chlubek D
[Ad] Address:Department of Maxillofacial Surgery, Hospital of the Ministry of Interior, Kielce, Wojska Polskiego 51, 25-375 Kielce, Poland. sikora-maciej@wp.pl.
[Ti] Title:In Vitro Effect of 3D Plates Used for Surgical Treatment of Condylar Fractures on Prostaglandin E2 (PGE2) and Thromboxane B2 (TXB2) Concentration in THP-1 Macrophages.
[So] Source:Int J Mol Sci;18(12), 2017 Dec 08.
[Is] ISSN:1422-0067
[Cp] Country of publication:Switzerland
[La] Language:eng
[Ab] Abstract:Recent studies have shown promising results concerning the effectiveness of 3D plates in terms of stabilization of condylar fractures. Despite the use of new techniques and new materials, we can still observe certain side effects, including the immune reaction of the body, which may lead to the excessive inflammation. The aim of this paper was to determine how the production of prostaglandin E2 (PGE2) and thromboxane B2 (TXB2) in THP-1 monocytes/macrophages is influenced by the titanium 3D plates and dedicated screws. The experiments were conducted on THP-1 monocytic cell line and macrophages derived from a THP-1cells. The concentrations of PGE2 and TXB2 released were measured by using immunoassay kit. Verification of plate-induced activation of THP-1 monocytes and macrophages and initiation of inflammatory reaction was conducted by flow cytometry. Despite some differences in the content of the implant devices our results showed that these plates did not statistically significantly increase the production of these prostanoids. Osteosynthesis of condylar fractures using 3D titanium mini-plates seems to be a good alternative to traditional plates due to their lack of stimulating the cyclooxygenase-dependent production of prostanoids; limiting the development of inflammatory reactions.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180109
[Lr] Last revision date:180109
[St] Status:In-Process

  10 / 9429 MEDLINE  
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[PMID]: 29305840
[Au] Autor:Gobo LA; de Carvalho LM; Temp F; Viana C; Mello CF
[Ad] Address:Federal University of Santa Maria (UFSM), Department of Chemistry, Campus universitário, Caixa Postal 5051, CEP 97105-900, Santa Maria, RS, Brazil.
[Ti] Title:A rapid method for identification and quantification of prostaglandins in cerebral tissues by HPLC-ESI-MS/MS for the lipidomic in vivo studies.
[So] Source:Anal Biochem;, 2018 Jan 03.
[Is] ISSN:1096-0309
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:An analytical method utilizing liquid chromatography coupled to mass spectrometry with electrospray ionization has been developed for the identification of prostaglandins (PGs) in cerebral tissues. The five compounds identified (thromboxane B2, prostaglandin E2, prostaglandin D2, 6-keto-prostaglandin F1 alpha and prostaglandin F2 alpha) are cellular mediators of inflammation and are involved in a variety of physiological and pathological processes by acting on membrane receptors on the surfaces of target cells. The parameters of the electrospray ionization interface were optimized to obtain the highest possible sensitivity for all compounds studied. The limits of detection ranged from 0.25 to 1.09 µg L , and the limits of quantification ranged from 0.83 to 3.64 µg L . The method was validated and applied to samples of brain tissue from five mice. The sample concentrations of the four prostaglandins quantified ranged from 375 ȵg L for prostaglandin E2 to 6602 µg L for prostaglandin D2. An advantage of this work that should be emphasized is the fast response of the method, which allows to obtaining the lipid profile after a 3 min chromatographic run.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180106
[Lr] Last revision date:180106
[St] Status:Publisher


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