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[PMID]: 29393458
[Au] Autor:Shan C; Ma Y
[Ad] Address:Department of Geriatrics, Weifang People's Hospital, Weifang, Shandong 261041, P.R. China.
[Ti] Title:MicroRNA-126/stromal cell-derived factor 1/C-X-C chemokine receptor type 7 signaling pathway promotes post-stroke angiogenesis of endothelial progenitor cell transplantation.
[So] Source:Mol Med Rep;17(4):5300-5305, 2018 Apr.
[Is] ISSN:1791-3004
[Cp] Country of publication:Greece
[La] Language:eng
[Ab] Abstract:Stroke is the most common cause of mortality worldwide. Post-stroke angiogenesis is of great significance to the treatment of strokes. The aim of the present study was to investigate the mechanism underlying the angiogenesis-promoting effect of microRNA­126 (miR­126)­associated signaling pathways using a stroke model in vivo and a cell migration model in vitro. Bone marrow­derived endothelial progenitor cells (EPCs) were extracted and identified using a density gradient method. Reverse transcription­quantitative polymerase chain reaction (RT­qPCR) was performed to examine the expression levels of miR­126 and C­X­C chemokine receptor type 7 (CXCR7). Target genes of miR­126 were analyzed using TargetScan software version 7.1 (www.targetscan.org/). In addition, a reporter gene assay and RT­qPCR were performed to determine the target genes of miR­126. The effect of miR­126 on cell migration was examined using a cell migration model in vitro and a middle cerebral artery occlusion model of mice was established in vivo. The miR­126 antagomir­treated EPCs were infused into stroke mice. Microvessel density, nerve function score and infarction volume were assessed. Flow cytometric analysis indicated that cluster of differentiation (CD)34, CD133 and vascular endothelial growth factor receptor 2 were partly expressed on the cell surface of bone marrow­derived EPCs. In addition, the expression levels of Di­acetylated­low density lipoprotein and Ulex europaeus agglutinin 1 were positive. Stromal cell­derived factor 1 (SDF-1) was identified as a target gene of miR­126, which was confirmed by a reporter gene assay and RT­qPCR. Cell migration examination demonstrated that the neutralizing antibody of CXCR7 blocked miR­126 angomir­induced migration of EPCs. Microvessel density increased, while nerve function score and infarction volume decreased following infusion of miR-126 angomir­treated EPCs. Furthermore, miR­126 angomir improved the efficacy of EPC treatment. Thus, miR­126 improved the migration of EPCs via the miR­126/SDF­1/CXCR7 signaling pathway.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:In-Process
[do] DOI:10.3892/mmr.2018.8513

  2 / 2058 MEDLINE  
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[PMID]: 29309596
[Au] Autor:Biswas Shivhare S; Bulmer JN; Innes BA; Hapangama DK; Lash GE
[Ad] Address:Reproductive and Vascular Biology Group, Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne NE2 4HH, UK.
[Ti] Title:Endometrial vascular development in heavy menstrual bleeding: altered spatio-temporal expression of endothelial cell markers and extracellular matrix components.
[So] Source:Hum Reprod;, 2018 Jan 04.
[Is] ISSN:1460-2350
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:STUDY QUESTION: Are there any phenotypic and structural/architectural changes in the vessels of endometrium and superficial myometrium during the normal menstrual cycle in healthy women and those with heavy menstrual bleeding (HMB)? SUMMARY ANSWER: Spatial and temporal differences in protein levels of endothelial cell (EC) markers and components of the extracellular matrix (ECM) were detected across the menstrual cycle in healthy women and these are altered in HMB. WHAT IS KNOWN ALREADY: HMB affects 30% of women of reproductive age with ~50% of cases being idiopathic. We have previously shown that the differentiation status of endometrial vascular smooth muscle cells (VSMCs) is altered in women with HMB, suggesting altered vessel maturation compared to controls. Endometrial arteriogenesis requires the co-ordinated maturation not only of the VSMCs but also the underlying ECs and surrounding ECM. We hypothesized that there are spatial and temporal patterns of protein expression of EC markers and vascular ECM components in the endometrium across the menstrual cycle, which are altered in women with HMB. STUDY DESIGN, SIZE, DURATION: Biopsies containing endometrium and superficial myometrium were taken from hysterectomy specimens from both healthy control women without endometrial pathology and women with subjective HMB in the proliferative (PP), early secretory (ESP), mid secretory (MSP) and late secretory (LSP) phases (N = 5 for each cycle phase and subject group). Samples were fixed in formalin and embedded in paraffin wax. PARTICIPANTS/MATERIALS, SETTING, METHODS: Serial sections (3µm thick) were immunostained for EC markers (factor VIII related antigen (F8RA), CD34, CD31 and ulex europaeus-agglutinin I (UEA-1) lectin), structural ECM markers (osteopontin, laminin, fibronectin and collagen IV) and for Ki67 to assess proliferation. Immunoreactivity of vessels in superficial myometrium, endometrial stratum basalis, stratum functionalis and luminal region was scored using either a modified Quickscore or by counting the number of positive vessels. MAIN RESULTS AND THE ROLE OF CHANCE: In control samples, all four EC markers showed a dynamic expression pattern according to the menstrual cycle phase, in both endometrial and myometrial vessels. EC protein marker expression was altered in women with HMB compared with controls, especially in the secretory phase in the endometrial luminal region and stratum functionalis. For example, in the LSP expression of UEA-1 and CD31 in the luminal region decreased in HMB (mean quickscore: 1 and 5, respectively) compared with controls (3.2 and 7.4, respectively) (both P = 0.008), while expression of F8RA and CD34 increased in HMB (1.4 and 8, respectively) compared with controls (0 and 5.8, respectively) (both P = 0.008). There was also a distinct pattern of expression of the vascular structural ECM protein components osteopontin, laminin, fibronectin and collagen IV in the superficial myometrium, stratum functionalis and stratum basalis during the menstrual cycle, which was altered in HMB. In particular, compared with controls, osteopontin expression in HMB was higher in stratum functionalis in the LSP (7.2 and 11.2, respectively P = 0.008), while collagen IV expression was reduced in stratum basalis in the MSP (4.6 and 2.8, respectively P = 0.002) and in stratum functionalis in the ESP (7 and 3.2, respectively P = 0.008). LIMITATIONS, REASONS FOR CAUTION: The protein expression of vascular EC markers and ECM components was assessed using a semi-quantitative approach in both straight and spiral arterioles. In our hospital, HMB is determined by subjective criteria and levels of blood loss were not assessed. WIDER IMPLICATIONS OF THE FINDINGS: Variation in the protein expression pattern between the four EC markers highlights the importance of choice of EC marker for investigation of endometrial vessels. Differences in expression of the different EC markers may reflect developmental stage dependent expression of EC markers in endometrial vessels, and their altered expression in HMB may reflect dysregulated vascular development. This hypothesis is supported by altered expression of ECM proteins within endometrial vessel walls, as well as our previous data showing a dysregulation in VSMC contractile protein expression in the endometrium of women with HMB. Taken together, these data support the suggestion that HMB symptoms are associated with weaker vascular structures, particularly in the LSP of the menstrual cycle, which may lead to increased and extended blood flow during menstruation. STUDY FUNDING/COMPETING INTEREST(S): This study was funded by Wellbeing of Women (RG1342) and Newcastle University. There are no competing interests to declare. TRIAL REGISTRATION NUMBER: Not applicable.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[St] Status:Publisher
[do] DOI:10.1093/humrep/dex378

  3 / 2058 MEDLINE  
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[PMID]: 29369788
[Au] Autor:Usó-Doménech JL; Nescolarde-Selva JA; Lloret-Climent M; González-Franco L
[Ad] Address:Department of Applied Mathematics, University of Alicante, Alicante, Spain.
[Ti] Title:Behavior of pyrophite shrubs in mediterranean terrestrial ecosystems (i): Population and reproductive model.
[So] Source:Math Biosci;297:58-77, 2018 Mar.
[Is] ISSN:1879-3134
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The mathematical submodel ULEX is used to study the dynamic behavior of the green, floral and woody biomass of the main pyrophite shrub species, the gorse (Ulex parviflorus Pourret), and its relationship with other shrub species, typical of a Mediterranean ecosystem. The focus are the ecological conditions of post-fire stage growth, and its efficacy as a protective cover against erosion processes in the short, medium and long term, both in normal conditions and at the limits of desertification conditions. The model sets a target to observe the behavior and to anticipate and consequently intervene with adequate protection, restoration and management measures.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180226
[Lr] Last revision date:180226
[St] Status:In-Data-Review

  4 / 2058 MEDLINE  
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[PMID]: 29462541
[Au] Autor:Pinilla-Gallego MS; Nieto V; Nates-Parra G
[Ti] Title:Recurso polínico y ciclo estacional de Thygater aethiops (Hymenoptera: Apidae) en un ambiente urbano (Bogotá-Colombia). [Pollen resource and seasonal cycle of Thygater aethiops (Hymenoptera: Apidae) in an urban environment (Bogotá-Colombia)].
[So] Source:Rev Biol Trop;64(3):1247-57, 2016 Sep.
[Is] ISSN:0034-7744
[Cp] Country of publication:Costa Rica
[La] Language:spa
[Ab] Abstract:Thygater aethiops is a native bee that can be found in parks and gardens in diverse urban areas such as those in the city of Bogotá (Colombia). However, little is known about its biology as well as ecological adaptations to urban areas. This study aimed to describe the seasonal cycle and daily foraging activities of T. aethiops, as well as identify the pollen resources used by this bee over a year in a population nesting in an aggregation in the "Parque Nacional Enrique Olaya Herrera" in Bogotá. Changes in the nest activity were monitored weekly by counting the number of active nests in the aggregation between December/2012 and February/2014. To determine the daily foraging activity, the numbers of bees entering their nests over a period of 10 minutes every hour between 8:00 and 14:00 h were recorded. Females with pollen loads entering to their nest were captured weekly, between September/2012 and August/2013, and their pollen loads analyzed. Three nesting peaks occurred after the precipitation peaks, but the number of active nests was not correlated with precipitation. The nesting activities stopped in a large number of the active nests (20-50 % of nests) after an anthropic disturbance was registered in the nesting area. Bees forage for nectar and pollen between 8:00 and 14:00 h, with a peak at 10:00 h. Daily foraging activity changed during the study period due to anthropic disturbance. There was not a significant relationship between air temperature and the number of females entering their nests. Foraging activities did not change between the dry and rainy seasons. A total of 26 pollen types were found in 169 pollen loads. Ulex europaeus (Fabaceae) and Solanum laxum (Solanaceae) were the most abundant plants represented on the pollen load across the study period. According to these results, T. aethiops would be considered a mesolectic species. The ability of T. aethiops to use different pollen resources both native and exotic, as well as to presumably recover its population after disturbances, are characteristics that may have allowed this bee to adapt to urban environments. Knowledge on the floral resources as well as other biological features of this bee species is important to promote its conservation in urban areas.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180220
[Lr] Last revision date:180220
[St] Status:In-Process

  5 / 2058 MEDLINE  
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[PMID]: 29357431
[Au] Autor:Qureshi SH; Patel NN; Murphy GJ
[Ad] Address:Cardiac surgery, Nottingham City Hospital, United Kingdom.
[Ti] Title:Vascular endothelial cell changes in post cardiac surgery acute kidney injury.
[So] Source:Am J Physiol Renal Physiol;, 2017 Dec 20.
[Is] ISSN:1522-1466
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Acute Kidney Injury (AKI) is common complication of cardiac surgery however the phenotype of this condition is poorly defined. The aim of this study was to characterize changes in endothelial structure and function that underlie post cardiopulmonary bypass (CPB) AKI. METHODS: Adult pigs (N=16) were randomised to undergo following procedures; (n=8 per group); Group 1: sham operation-neck dissection with 2.5hr of general anesthesia and Group 2: CPB-2.5hr of cardiopulmonary bypass. RESULTS: CPB resulted in the depletion of specific epitopes of glycosaminoglycans side chains of the endothelial glycocalyx; Dolichos biflorus agglutinin; mean difference (MD)(95% Confidence Interval (CI)) P value: -0.26 (-0.42, -0.09), P=0.0024, Triticum vulgaris (wheat germ) agglutinin: -0.83(-1.2, -0.38), P=0.0005 and Ulex europaeus agglutinin 1: -0.25(-0.49, -0.009), P=0.041, endothelial membrane proteins; thrombomodulin: -3.13(-5.6, -0.65), P=0.02 and adherens junctions; VE-Cadherin: -1.06(-1.98, -0.145), P=0.02. CPB also resulted in reductions in microvascular cortical perfusion: -0.62(-1.02, -0.22), P= 0.006, and increased renal cortex adenosine levels: 2.32(0.83, 3.8), P=0.0059. These changes were accompanied by significant reduction in creatinine clearance at 1.5hr post intervention, mean difference (MD) 95% confidence interval (CI); -51.7 (-99.7, -3.7), P=0.037 and at 24hr, MD (95% CI): -47.3 (-87.7, -7.6), P= 0.023, and proteinuria immediately post intervention MD (95%CI): 18.79 (2.17, 35.4), P= 0.03 vs. sham. CONCLUSION: In our experimental CPB model, endothelial injury was associated with loss of autoregulation, increase in microvascular permeability and reduced glomerular filtration. Interventions that promote endothelial homeostasis may have clinical utility in the prevention of post cardiac surgery AKI.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180131
[Lr] Last revision date:180131
[St] Status:Publisher
[do] DOI:10.1152/ajprenal.00319.2017

  6 / 2058 MEDLINE  
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[PMID]: 29237495
[Au] Autor:Zhang S; Ma X; Guo J; Yao K; Wang C; Dong Z; Zhu H; Fan F; Huang Z; Yang X; Qian J; Zou Y; Sun A; Ge J
[Ad] Address:Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China.
[Ti] Title:Bone marrow CD34 cell subset under induction of moderate stiffness of extracellular matrix after myocardial infarction facilitated endothelial lineage commitment in vitro.
[So] Source:Stem Cell Res Ther;8(1):280, 2017 Dec 13.
[Is] ISSN:1757-6512
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: The stiffness of the myocardial extracellular matrix (ECM) and the transplanted cell type are vitally important in promoting angiogenesis. However, the combined effect of the two factors remains uncertain. The purpose of this study is to investigate in vitro the combined effect of myocardial ECM stiffness postinfarction with a bone marrow-derived cell subset expressing or not expressing CD34 on endothelial lineage commitment. METHODS: Myocardial stiffness of the infarct zone was determined in mice at 1 h, 24 h, 7 days, 14 days, and 28 days after coronary artery ligation. Polyacrylamide (PA) gel substrates of different stiffnesses were prepared to mechanically mimic the myocardial ECM after infarction. Mouse bone marrow-derived CD34 and CD34 cells were seeded on the flexible PA gels. The double-positive expression for DiI-acetylated low-density lipoprotein (acLDL) uptake and fluorescein isothiocyanate-Ulex europaeus agglutinin-1 (FITC-UEA-1) binding, the endothelial lineage antigens CD31, von Willebrand factor (vWF), Flk-1, and VE-cadherin, as well as cytoskeleton were measured by immunofluorescent staining on day 7. Cell apoptosis was evaluated by both immunofluorescent staining and flow cytometry at 24 h after culture. RESULTS: We found that the numbers of the CD34 cell subset adherent to the flexible substrates (4-72 kPa) was much larger than that of the CD34 subset. More double-positive cells for DiI-acLDL uptake/FITC-UEA-1 binding were seen on the 42-kPa (moderately stiff) substrate, corresponding to the stiffness of myocardial ECM at 7-14 days postinfarction, compared with those on substrates of other stiffnesses. Similarly, the moderately stiff substrate showed benefits in promoting the positive expressions of the endothelial lineage markers CD31, vWF, Flk-1, and VE-cadherin. In addition, the cytoskeleton F-actin network within CD34 cells was organized more significantly at the leading edge of the adherent cells on the moderately stiff (42 kPa) or stiff (72 kPa) substrates as compared with those on the soft (4 kPa and 15 kPa) substrates. Moreover, the moderately stiff or stiff substrates showed a lower percentage of cell apoptosis than the soft substrates. CONCLUSIONS: Infarcted myocardium-like ECM of moderate stiffness (42 kPa) more beneficially regulated the endothelial lineage commitment of a bone marrow-derived CD34 subset. Thus, the combination of a CD34 subset with a "suitable" ECM stiffness might be an optimized strategy for cell-based cardiac repair.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 171220
[Lr] Last revision date:171220
[St] Status:In-Process
[do] DOI:10.1186/s13287-017-0732-x

  7 / 2058 MEDLINE  
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[PMID]: 29075475
[Au] Autor:La Pierre KJ; Simms EL; Tariq M; Zafar M; Porter SS
[Ad] Address:Department of Integrative BiologyUniversity of CaliforniaBerkeleyCAUSA.
[Ti] Title:Invasive legumes can associate with many mutualists of native legumes, but usually do not.
[So] Source:Ecol Evol;7(20):8599-8611, 2017 Oct.
[Is] ISSN:2045-7758
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Mutualistic interactions can strongly influence species invasions, as the inability to form successful mutualisms in an exotic range could hamper a host's invasion success. This barrier to invasion may be overcome if an invader either forms novel mutualistic associations or finds and associates with familiar mutualists in the exotic range. Here, we ask (1) does the community of rhizobial mutualists associated with invasive legumes in their exotic range overlap with that of local native legumes and (2) can any differences be explained by fundamental incompatibilities with particular rhizobial genotypes? To address these questions, we first characterized the rhizobial communities naturally associating with three invasive and six native legumes growing in the San Francisco Bay Area. We then conducted a greenhouse experiment to test whether the invasive legume could nodulate with any of a broad array of rhizobia found in their exotic range. There was little overlap between the communities associated with wild-grown invasive and native legumes, yet the invasive legumes could nodulate with a broad range of rhizobial strains under greenhouse conditions. These observations suggest that under field conditions in their exotic range, these invasive legumes are not currently associating with the mutualists of local native legumes, despite their potential to form such associations. However, the promiscuity with which these invading legumes can form mutualistic associations could be an important factor early in the invasion process if mutualist scarcity limits range expansion. Overall, the observation that invasive legumes have a community of rhizobia distinct from that of native legumes, despite their ability to associate with many rhizobial strains, challenges existing assumptions about how invading species obtain their mutualists. These results can therefore inform current and future efforts to prevent and remove invasive species.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1710
[Cu] Class update date: 171029
[Lr] Last revision date:171029
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.1002/ece3.3310

  8 / 2058 MEDLINE  
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[PMID]: 28892824
[Au] Autor:Lippestad M; Hodges RR; Utheim TP; Serhan CN; Dartt DA
[Ad] Address:Schepens Eye Research Institute, Massachusetts Eye and Ear Infirmary, Department of Ophthalmology, Harvard Medical School, Boston, Massachusetts, United States.
[Ti] Title:Resolvin D1 Increases Mucin Secretion in Cultured Rat Conjunctival Goblet Cells via Multiple Signaling Pathways.
[So] Source:Invest Ophthalmol Vis Sci;58(11):4530-4544, 2017 Sep 01.
[Is] ISSN:1552-5783
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Purpose: Goblet cells in the conjunctiva secrete mucin into the tear film protecting the ocular surface. The proresolution mediator resolvin D1 (RvD1) regulates mucin secretion to maintain homeostasis during physiological conditions and in addition, actively terminates inflammation. We determined the signaling mechanisms used by RvD1 in cultured rat conjunctival goblet cells to increase intracellular [Ca2+] ([Ca2+]i) and induce glycoconjugate secretion. Methods: Increase in [Ca2+]i were measured using fura 2/AM and glycoconjugate secretion determined using an enzyme-linked lectin assay with the lectin Ulex Europaeus Agglutinin 1. Signaling pathways activated by RvD1 were studied after goblet cells were pretreated with signaling pathway inhibitors before stimulation with RvD1. The results were compared with results when goblet cells were stimulated with RvD1 alone and percent inhibition calculated. Results: The increase in [Ca2+]i stimulated by RvD1 was blocked by inhibitors to phospholipases (PL-) -D, -C, -A2, protein kinase C (PKC), extracellular signal-regulated kinases (ERK)1/2 and Ca2+/calmodulin-dependent kinase (Ca2+/CamK). Glycoconjugate secretion was significantly inhibited by PLD, -C, -A2, ERK1/2 and Ca2+/CamK, but not PKC. Conclusions: We conclude that RvD1 increases glycoconjugate secretion from goblet cells via multiple signaling pathways including PLC, PLD, and PLA2, as well as their signaling components ERK1/2 and Ca2+/CamK to preserve the mucous layer and maintain homeostasis by protecting the eye from desiccating stress, allergens, and pathogens.
[Mh] MeSH terms primary: Conjunctiva/drug effects
Docosahexaenoic Acids/pharmacology
Goblet Cells/drug effects
Mucins/secretion
Signal Transduction/physiology
[Mh] MeSH terms secundary: Animals
Calcium/metabolism
Cells, Cultured
Conjunctiva/metabolism
Enzyme-Linked Immunosorbent Assay
Fura-2/analogs & derivatives
Fura-2/metabolism
Goblet Cells/metabolism
Inositol 1,4,5-Trisphosphate Receptors/metabolism
MAP Kinase Signaling System/physiology
Male
Phospholipase D/metabolism
Phospholipases A2/metabolism
Rats
Rats, Sprague-Dawley
Receptors, Lipoxin/metabolism
Type C Phospholipases/metabolism
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Inositol 1,4,5-Trisphosphate Receptors); 0 (Mucins); 0 (Receptors, Lipoxin); 0 (lipoxin A(4) receptor, rat); 0 (resolvin D1); 105344-37-4 (fura-2-am); 25167-62-8 (Docosahexaenoic Acids); EC 3.1.1.4 (Phospholipases A2); EC 3.1.4.- (Type C Phospholipases); EC 3.1.4.4 (Phospholipase D); SY7Q814VUP (Calcium); TSN3DL106G (Fura-2)
[Em] Entry month:1709
[Cu] Class update date: 170929
[Lr] Last revision date:170929
[Js] Journal subset:IM
[Da] Date of entry for processing:170912
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-21914

  9 / 2058 MEDLINE  
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[PMID]: 28513999
[Au] Autor:Alexandru N; Andrei E; Niculescu L; Dragan E; Ristoiu V; Georgescu A
[Ad] Address:Institute of Cellular Biology and Pathology 'Nicolae Simionescu' of the Romanian Academy, Bucharest, Romania.
[Ti] Title:Microparticles of healthy origins improve endothelial progenitor cell dysfunction via microRNA transfer in an atherosclerotic hamster model.
[So] Source:Acta Physiol (Oxf);, 2017 May 17.
[Is] ISSN:1748-1716
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:AIM: In this study, we aimed: (i) to obtain and functionally characterize the cultures of late endothelial progenitor cells (EPCs) from the animal blood; (ii) to investigate the potential beneficial effects of circulating microparticles (MPs) of healthy origins on EPC dysfunctionality in atherosclerosis as well as involved mechanisms. METHODS: Late EPCs were obtained and expanded in culture from peripheral blood isolated from two animal groups: hypertensive-hyperlipidaemic (HH) and control (C) hamsters. In parallel experiments, late EPC cultures from HH were incubated with MPs from C group. RESULTS: The results showed that late EPCs display endothelial cell phenotype: (i) have ability to uptake 1,1-dioctadecyl-3,3,3,3 tetramethylindocarbocyanine-labelled acetylated low-density lipoprotein and Ulex europaeus agglutinin lectin-1; (ii) express CD34, CD133, KDR, CD144, vWF, Tie-2. Late EPCs from HH exhibited different morphological and functional characteristics compared to control: (i) are smaller and irregular in shape; (ii) present decreased endothelial surface marker expression; (iii) display reduced proliferation, migration and adhesion; (iv) lose ability to organize themselves into tubular structures and integrate into vascular network; (v) have diminished function of inward rectifier potassium channels. The incubation of late EPCs with MPs improved EPC functionality by miR-10a, miR-21, miR-126, miR-146a, miR-223 transfer and IGF-1 expression activation; the kinetic study of MP incorporation into EPCs demonstrated MP uptake by EPCs followed by the miRNA transfer. CONCLUSION: The data reveal that late EPCs from atherosclerotic model exhibit distinctive features and are dysfunctional, and their function recovery can be supported by MP ability to transfer miRNAs. These findings bring a new light on the vascular repair in atherosclerosis.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1705
[Cu] Class update date: 170608
[Lr] Last revision date:170608
[St] Status:Publisher
[do] DOI:10.1111/apha.12896

  10 / 2058 MEDLINE  
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[PMID]: 28396600
[Au] Autor:Kang KT; Lin RZ; Kuppermann D; Melero-Martin JM; Bischoff J
[Ad] Address:Vascular Biology Program and Department of Surgery, Boston Children's Hospital, Harvard Medical School, Boston, MA, 02115, USA.
[Ti] Title:Endothelial colony forming cells and mesenchymal progenitor cells form blood vessels and increase blood flow in ischemic muscle.
[So] Source:Sci Rep;7(1):770, 2017 Apr 10.
[Is] ISSN:2045-2322
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Here we investigated whether endothelial colony forming cells (ECFC) and mesenchymal progenitor cells (MPC) form vascular networks and restore blood flow in ischemic skeletal muscle, and whether host myeloid cells play a role. ECFC + MPC, ECFC alone, MPC alone, or vehicle alone were injected into the hind limb ischemic muscle one day after ligation of femoral artery and vein. At day 5, hind limbs injected with ECFC + MPC showed greater blood flow recovery compared with ECFC, MPC, or vehicle. Tail vein injection of human endothelial specific Ulex europaeus agglutinin-I demonstrated an increased number of perfused human vessels in ECFC + MPC compared with ECFC. In vivo bioluminescence imaging showed ECFC persisted for 14 days in ECFC + MPC-injected hind limbs. Flow cytometric analysis of ischemic muscles at day 2 revealed increased myeloid lineage cells in ECFC + MPC-injected muscles compared to vehicle-injected muscles. Neutrophils declined by day 7, while the number of myeloid cells, macrophages, and monocytes did not. Systemic myeloid cell depletion with anti-Gr-1 antibody blocked the improved blood flow observed with ECFC + MPC and reduced ECFC and MPC retention. Our data suggest that ECFC + MPC delivery could be used to reestablish blood flow in ischemic tissues, and this may be enhanced by coordinated recruitment of host myeloid cells.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1704
[Cu] Class update date: 170517
[Lr] Last revision date:170517
[St] Status:In-Data-Review
[do] DOI:10.1038/s41598-017-00809-1


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