Database : MEDLINE Search on : ancylostomiasis [Words] References found : 1036 [refine] Displaying: 1 .. 10   in format [Detailed]

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select to print Photocopy SciELO Brazil full text SciELO Public Health full text

[PMID]: 23503538 [Au] Autor: Gazzinelli MF; de Souza V; de Araújo LH; Costa Rde M; Soares AN; Maia CP [Ad] Address: Departamento de Enfermagem Aplicada, Escola de Enfermagem, Universidade, Federal de Minas Gerais, 190 30130-100 Belo Horizonte, MG, Brasil. flaviagazzinelli@yahoo.com.br [Ti] Title: Teatro na educação de crianças e adolescentes participantes de ensaio clínico. [Theater in the education of children and teenagers participating in a clinical trial]. [So] Source: Rev Saude Publica;46(6):999-1006, 2012 Dec. [Is] ISSN: 1518-8787 [Cp] Country of publication: Brazil [La] Language: por [Ab] Abstract: OBJECTIVE: To analyze the effects of a pedagogical intervention on the learning of children and teenagers participating in a clinical research. METHODS: Quantitative, quasi-experimental and longitudinal study, part of a group of studies conducted to test a vaccine against ancylostomiasis. Convenience sample with 133 students aged 10-17 years, of both sexes, from the school Escola Municipal de Maranhão (Southeastern Brazil), 2009. A structured questionnaire was used, which was administered before and after the intervention. The pedagogical device was the "Theater of the Oppressed". The dependent variables were specific and global knowledge about clinical research and about parasitic worms; the independent variable was participation in the educational intervention. RESULTS: There was an increase in knowledge about signals and symptoms, susceptibility to reinfection and way of contagion after the educational intervention. We observed an increase in the number of right answers concerning duration of clinical research, procedures, the possibility of quitting participation, and occurrence of adverse events. The notion that the research's primary purpose is therapeutic remained, but the percentage of participants who associated the research with medical treatment decreased. The "Theater of the Oppressed" enabled that the discussions about helminthiasis and clinical research were contextualized and materialized. The subjects could dispose of or reduce their previous representations. CONCLUSIONS: Participation of children and adolescents in clinical trials must be preceded by an educational intervention, since individuals of that age group do not even recognize they have the right to decide for themselves. [Pt] Publication type: ENGLISH ABSTRACT; JOURNAL ARTICLE [Em] Entry month: 1303 [Js] Journal subset: IM [St] Status: In-Process

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[PMID]: 23232252 [Au] Autor: Davey D; Manickam N; Simms BT; Harrison LM; Vermeire JJ; Cappello M [Ad] Address: Program in International Child Health, Department of Pediatrics, Yale School of Medicine, New Haven, CT 06520, USA. [Ti] Title: Frequency and intensity of exposure mediate resistance to experimental infection with the hookworm, Ancylostoma ceylanicum. [So] Source: Exp Parasitol;133(3):243-9, 2013 Mar. [Is] ISSN: 1090-2449 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Hookworms are bloodfeeding intestinal nematodes that are a major cause of anemia in resource-limited countries. Despite repeated exposure beginning in early childhood, humans retain lifelong susceptibility to infection without evidence of sterilizing immunity. In contrast, experimental infection of laboratory animals is typically characterized by varying degrees of resistance following primary infection, although the mechanisms underlying this phenomenon remain unknown. In this study, hamsters subjected to a single drug-terminated infection with 100 third stage hookworm larvae were confirmed to be resistant to pathological effects following a subsequent challenge. In a second experiment, hamsters infected twice-weekly with 10 third stage larvae (low inoculum) exhibited clinical and parasitological evidence of continued susceptibility, while those given 100 L3 (high inoculum) developed apparent resistance within 3 days following the initial exposure. The kinetics of parasite-specific IgA, IgM, and IgG antibody production varied by group, which suggests that the humoral immune response to hookworm infection is stimulated by the nature (frequency and intensity) of larval exposure. These results suggest that intermittent low-inoculum larval exposure, which is characterized by prolonged susceptibility to infection, may serve as a more representative model of human hookworm disease for studies of pathogenesis, as well as drug and vaccine development. [Mh] MeSH terms primary: Ancylostoma/immunology Ancylostomiasis/immunology Antibodies, Helminth/blood [Mh] MeSH terms secundary: Ancylostomiasis/complications Anemia/parasitology Animals Antigens, Helminth/diagnostic use Antigens, Helminth/immunology Cricetinae Disease Models, Animal Disease Resistance Feces/chemistry Feces/parasitology Hemoglobins/analysis Immunoglobulin A, Secretory/biosynthesis Immunoglobulin G/blood Immunoglobulin M/blood Intestines/immunology Intestines/parasitology Lymph Nodes/pathology Male Mesentery Mesocricetus Parasite Egg Count Spleen/anatomy & histology [Pt] Publication type: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Name of substance: 0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Hemoglobins); 0 (Immunoglobulin A, Secretory); 0 (Immunoglobulin G); 0 (Immunoglobulin M) [Em] Entry month: 1304 [Cu] Class update date: 130416 [Lr] Last revision date: 130416 [Js] Journal subset: IM [Da] Date of entry for processing: 130222 [St] Status: MEDLINE

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[PMID]: 22869629 [Au] Autor: Barakat M; Ibrahim N; Nasr A [Ad] Address: Department of Tropical Medicine and Gastroenterology, Assiut University Hospital, Assiut, Egypt. mahabarakat2001@yahoo.com [Ti] Title: In vivo endoscopic imaging of ancylostomiasis-induced gastrointestinal bleeding: clinical and biological profiles. [So] Source: Am J Trop Med Hyg;87(4):701-5, 2012 Oct. [Is] ISSN: 1476-1645 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Little data are available regarding the association of ancylostomiasis with overt gastrointestinal bleeding. This 6-year retrospective study describes the clinical and biological profiles of unexpectedly identified ancylostomiasis in a 4-month-old baby and four adults; they presented with melena and were referred for urgent diagnostic gastrointestinal endoscopy, which confirmed numerous small intestine injuries with surrounding blood pools caused by Ancylostoma duodenale worms. Gastric erosions were also encountered in one patient. Uniquely, worm biological activities were recorded live in vivo, including mucosal invasion through a vigorous, rapid piercing process, repeated bloodsucking habits, and gut appearance during the stages of feeding, digestion, and excretion in male and female worms. In conclusion, ancylostomiasis-induced melena may occur in all ages from infants to the elderly. Worm bloodfeeding occurs after quick mucosal piercing, with blood loss being aggravated by a repeated feeding behavior. After treatment is started, bleeding stops rapidly in response to anthelmintic therapy. [Mh] MeSH terms primary: Ancylostoma/physiology Ancylostomiasis/complications Endoscopy, Gastrointestinal/methods Gastrointestinal Hemorrhage/etiology Intestine, Small/pathology [Mh] MeSH terms secundary: Adult Aged Aged, 80 and over Ancylostomiasis/drug therapy Ancylostomiasis/parasitology Animals Anthelmintics/administration & dosage Anthelmintics/therapeutic use Female Gastrointestinal Hemorrhage/diagnosis Gastrointestinal Hemorrhage/pathology Humans Infant Intestine, Small/parasitology Male Melena/drug therapy Melena/etiology Middle Aged Young Adult [Pt] Publication type: JOURNAL ARTICLE [Nm] Name of substance: 0 (Anthelmintics) [Em] Entry month: 1212 [Cu] Class update date: 130416 [Lr] Last revision date: 130416 [Js] Journal subset: AIM; IM [Da] Date of entry for processing: 121008 [St] Status: MEDLINE [do] DOI: 10.4269/ajtmh.2012.12-0018

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[PMID]: 23026558 [Au] Autor: Taweethavonsawat P; Chungpivat S; Watanapongchati S; Traub RJ; Schaper R [Ad] Address: Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. Piyanan.T@chula.ac.th [Ti] Title: Comparative efficacy of a spot-on formulation containing emodepside and praziquantel (Profender ®, Bayer) and praziquantel and pyrantel oral tablets (Drontal ® for Cats) against experimental Ancylostoma ceylanicum infections in cats. [So] Source: Vet Parasitol;191(1-2):172-6, 2013 Jan 16. [Is] ISSN: 1873-2550 [Cp] Country of publication: Netherlands [La] Language: eng [Ab] Abstract: Ancylostoma ceylanicum is a common zoonotic hookworm of dogs and cats throughout Asia and has also been reported to occur within the Australasian region. The aim of this study to was to determine the efficacy of a spot-on formulation containing emodepside and praziquantel (Profender(®), Bayer) and praziquantel and pyrantel oral tablets (Drontal(®) for Cats, Bayer) against experimental A. ceylanicum infections in cats. Twenty-four kittens were each subcutaneously injected with 100 infective third-stage larvae of A. ceylanicum. Kittens were stratified by egg count and randomly allocated equally into control and two treatment groups. The first group were treated with emodepside 2.1%/praziquantel 8.6% (Profender®, Bayer) at the recommended label dose. The second group was treated with 80 mg pyrantel and 20mg praziquantel (Drontal(®) for Cats, Bayer) at the recommended label dose. The kittens in the control group were not treated. Egg counts were performed daily until the end of the study period and compared for the treated and control groups. No eggs were detected in the treated group of kittens within 4 days of treatment and faecal samples from this group remained negative throughout the rest of the study, resulting in a treatment efficacy (egg reduction) of 100% (P<0.0001). The egg counts remained high (993 ± 666 epg) in the untreated control group for the rest of the study period. This study demonstrated that both combination products containing topical emodepside/praziquantel (Profender(®), Bayer) and praziquantel/pyrantel oral tablets (Drontal(®) for Cats, Bayer) given at the recommended dose is highly effective against infection with A. ceylanicum in cats. [Mh] MeSH terms primary: Ancylostomiasis/veterinary Antinematodal Agents/therapeutic use Depsipeptides/therapeutic use Praziquantel/therapeutic use Pyrantel/therapeutic use [Mh] MeSH terms secundary: Ancylostomiasis/drug therapy Animals Cat Diseases/drug therapy Cats Drug Combinations Feces/parasitology Random Allocation Treatment Outcome [Pt] Publication type: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Name of substance: 0 (Antinematodal Agents); 0 (Depsipeptides); 0 (Drug Combinations); 0 (emodepside); 15686-83-6 (Pyrantel); 55268-74-1 (Praziquantel) [Em] Entry month: 1304 [Js] Journal subset: IM [Da] Date of entry for processing: 121119 [St] Status: MEDLINE

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select to print Photocopy PubMed Central Full text Full text

[PMID]: 23145203 [Au] Autor: Hu Y; Zhan B; Keegan B; Yiu YY; Miller MM; Jones K; Aroian RV [Ad] Address: Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California, United States of America. bzhan@bcm.edu [Ti] Title: Mechanistic and single-dose in vivo therapeutic studies of Cry5B anthelmintic action against hookworms. [So] Source: PLoS Negl Trop Dis;6(11):e1900, 2012. [Is] ISSN: 1935-2735 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: BACKGROUND: Hookworm infections are one of the most important parasitic infections of humans worldwide, considered by some second only to malaria in associated disease burden. Single-dose mass drug administration for soil-transmitted helminths, including hookworms, relies primarily on albendazole, which has variable efficacy. New and better hookworm therapies are urgently needed. Bacillus thuringiensis crystal protein Cry5B has potential as a novel anthelmintic and has been extensively studied in the roundworm Caenorhabditis elegans. Here, we ask whether single-dose Cry5B can provide therapy against a hookworm infection and whether C. elegans mechanism-of-action studies are relevant to hookworms. METHODOLOGY/PRINCIPAL FINDINGS: To test whether the C. elegans invertebrate-specific glycolipid receptor for Cry5B is relevant in hookworms, we fed Ancylostoma ceylanicum hookworm adults Cry5B with and without galactose, an inhibitor of Cry5B-C. elegans glycolipid interactions. As with C. elegans, galactose inhibits Cry5B toxicity in A. ceylanicum. Furthermore, p38 mitogen-activated protein kinase (MAPK), which controls one of the most important Cry5B signal transduction responses in C. elegans, is functionally operational in hookworms. A. ceylanicum hookworms treated with Cry5B up-regulate p38 MAPK and knock down of p38 MAPK activity in hookworms results in hypersensitivity of A. ceylanicum adults to Cry5B attack. Single-dose Cry5B is able to reduce by >90% A. ceylanicum hookworm burdens from infected hamsters, in the process eliminating hookworm egg shedding in feces and protecting infected hamsters from blood loss. Anthelmintic activity is increased about 3-fold, eliminating >97% of the parasites with a single 3 mg dose (∼30 mg/kg), by incorporating a simple formulation to help prevent digestion in the acidic stomach of the host mammal. CONCLUSIONS/SIGNIFICANCE: These studies advance the development of Cry5B protein as a potent, safe single-dose anthelmintic for hookworm therapy and make available the information of how Cry5B functions in C. elegans in order to study and improve Cry5B function against hookworms. [Mh] MeSH terms primary: Ancylostoma/drug effects Ancylostomiasis/drug therapy Anthelmintics/administration & dosage Bacterial Proteins/administration & dosage Endotoxins/administration & dosage Hemolysin Proteins/administration & dosage [Mh] MeSH terms secundary: Animals Cricetinae Disease Models, Animal Feces/parasitology Female Male Mesocricetus Parasite Egg Count Treatment Outcome [Pt] Publication type: JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Name of substance: 0 (Anthelmintics); 0 (Bacterial Proteins); 0 (Endotoxins); 0 (Hemolysin Proteins); 0 (insecticidal crystal protein, Bacillus Thuringiensis) [Em] Entry month: 1304 [Js] Journal subset: IM [Da] Date of entry for processing: 121112 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pntd.0001900

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[PMID]: 20302705 [Au] Autor: Huang DN; Chen MX; Geng YJ; Li XH; Gao ST; Zhang RL [Ad] Address: Shenzhen Centre for Disease Control and Prevention, Shenzhen 518020, China. [Ti] Title: [Detection of circulating antigen of Angiostrongylus cantonensis by 12D5 and 21B7 monoclonal antibodies]. [So] Source: Zhonghua Liu Xing Bing Xue Za Zhi;31(1):79-82, 2010 Jan. [Is] ISSN: 0254-6450 [Cp] Country of publication: China [La] Language: chi [Ab] Abstract: OBJECTIVE: To detect the rate of Angiostrongylus cantonensis infection and to study the effects of treatment so as to prepare monoclonal antibodies (McAbs), and gold immunochromatography assay (GICA) with 12D5 and 21B7 McAbs could be prepared in advance. METHODS: Two McAbs (12D5 and 21B7) were applied to detect the circulating antigen (CAg) in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively by double antibody sandwich ELISA. Either 12D5 or 21B7 McAbs was used as antibody and protein A was conjugated with colloid gold as the detection marker. A special pad for GICA was designed according to the reaction procedure, and CAg were detected by GICA in the sera of rats infected with A. cantonensis and angiostrongyliasis patients respectively. RESULTS: 12D5 McAb was identified as IgG1 and 21B7 McAb was IgM. Results from Western blotting showed that two McAbs could be used to identified 55 KD protein of adult worms of A. cantonensis. The detection rates of CAg in the sera of infected rats was 100% (48/48) and the detection rates of CAg in the sera of angiostrongyliasis patients was 100% (32/32). No cross-reaction to sera of patients with other infection of parasites, such as clonorchiasis, fasciolopsiasis, ancylostomiasis, ancylostomiasis, anisakiasis as well as schistosomiasis wee seen and normal sera did not react with 12D5 and 21B7 McAbs. CONCLUSION: Results from sandwich ELISA and GICA with 12D5 and 21B7 McAbs showed high specificity and acting as detecting CAg of A. cantonensis in sera of infected animals and patients. We noticed that GICA with 12D5 and 21B7 was not only rapid and simple that without requirement of special instrument, but also rather sensitive and specific for the detection of current infection with A. cantonensis. [Mh] MeSH terms primary: Angiostrongylus cantonensis/immunology Antibodies, Monoclonal/diagnostic use Antigens, Helminth/blood Strongylida Infections/blood [Mh] MeSH terms secundary: Animals Antibodies, Helminth/immunology Antibodies, Monoclonal/immunology Enzyme-Linked Immunosorbent Assay/methods Female Male Rats Rats, Sprague-Dawley [Pt] Publication type: ENGLISH ABSTRACT; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Name of substance: 0 (Antibodies, Helminth); 0 (Antibodies, Monoclonal); 0 (Antigens, Helminth) [Em] Entry month: 1303 [Js] Journal subset: IM [Da] Date of entry for processing: 100324 [St] Status: MEDLINE

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[PMID]: 22661075 [Au] Autor: Agarwal P; Singh D; Sinha G; Sharma N; Titiyal JS [Ad] Address: Dr R.P. Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi, India. [Ti] Title: Bilateral Mooren's ulcer in a child secondary to helminthic infestation of the gastrointestinal tract. [So] Source: Int Ophthalmol;32(5):463-6, 2012 Oct. [Is] ISSN: 1573-2630 [Cp] Country of publication: Netherlands [La] Language: eng [Ab] Abstract: To report an unusual association of bilateral Mooren's ulcer in a child with helminthic infestation of gastrointestinal tract. A 6-year-old female presented with redness, watering and photophobia in left eye for 2 months and in right eye for 2 weeks. BCVA was 20/200 in OD and 20/400 OS. Superior peripheral corneal ulcer of 8 × 2 mm was present in the right eye and 8 × 3 mm perforated limbal corneal ulcer with staphyloma was present in the left eye. Hemogram revealed microcytic hypochromic anemia, eosinophilia and elevated ESR. No organism was isolated on corneal scraping. Stool examination revealed presence of Ancylostoma duodenale. Therapy included fortified topical antibiotics, cycloplegics, lubricants and oral albendazole. Conjunctival recession and crescentic therapeutic penetrating keratoplasty was done in OD and OS respectively. At 18 months follow up, there was no recurrence in any of the eyes. Bilateral Mooren's ulcer may be present with gastrointestinal hookworm infestation. Prompt and appropriate management may provide optimal therapeutic success. [Mh] MeSH terms primary: Ancylostoma/isolation & purification Ancylostomiasis/parasitology Corneal Ulcer/parasitology Eye Infections, Parasitic/parasitology Gastrointestinal Tract/parasitology Hookworm Infections/parasitology Intestinal Diseases, Parasitic/complications [Mh] MeSH terms secundary: Ancylostomiasis/diagnosis Animals Child Corneal Ulcer/diagnosis Diagnosis, Differential Eye Infections, Parasitic/diagnosis Female Hookworm Infections/diagnosis Humans Intestinal Diseases, Parasitic/parasitology [Pt] Publication type: CASE REPORTS; JOURNAL ARTICLE [Em] Entry month: 1303 [Js] Journal subset: IM [Da] Date of entry for processing: 120927 [St] Status: MEDLINE

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[PMID]: 23142084 [Au] Autor: Dias SR; da Costa AF; Gazzinelli-Guimarães PH; Roatt BM; da Silva Fonseca K; de Paiva NC; Giunchetti RC; Carneiro CM; Fujiwara RT; Rabelo ÉM [Ad] Address: Laboratório de Parasitologia Molecular, Departamento de Parasitologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627, Pampulha, CEP 31270-901, Belo Horizonte, Minas Gerais, Brazil. [Ti] Title: Prednisolone and cyclosporine A: effects on an experimental model of ancylostomiasis. [So] Source: Exp Parasitol;133(1):80-8, 2013 Jan. [Is] ISSN: 1090-2449 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Corticosteroids and cyclosporine A (CsA) are important clinical immunosuppressive drugs used in the maintenance of organ transplants and in suppressing undesired autoimmune or allergic immune responses. To study the effect of CsA and prednisolone on the course of an Ancylostoma ceylanicum infection, hamsters were treated with commercially available prednisolone or CsA. For both drugs, half the recommended dose was sufficient to inhibit the proliferation of more than 70% of hamster lymph node cells. There was no difference in the recovery of adult worms; however, animals treated with prednisolone presented with low egg counts in the feces. Infection with A. ceylanicum resulted in an increase in specific antibodies against adult worm antigens, but hamsters treated with either drug presented with lower IgG titers. We observed that A. ceylanicum infection caused peripheral cellular immune suppression, which is characterized by a reduction in the total white cell count, neutropenia and lymphopenia. We also observed a lymphoplasmacytic pattern and few eosinophils in the mucosal inflammatory infiltrate for all the animals. The animals treated with prednisolone showed changes in the architecture of the intestine, including the loss of the mucosa, intense congestion and inflammation. In spleen, we observed hyperplasia of white pulp in all infected animals; in addition, there was a loss of tissue architecture in the animals treated with prednisolone. In conclusion, this work shows that an A. ceylanicum infection leads to acute peripheral cellular immune suppression in hamsters but not humoral immune suppression and that CsA treatment does not interfere with the process of infection. However, prednisolone treatment causes intestinal injury, what could hamper the parasite attachment to the intestinal wall, and as a result affects copulation and, consequently, decreases the number of eggs eliminated in the feces. Moreover, the possibility that the drug can also be exerting an effect on female fertility should be considered. [Mh] MeSH terms primary: Ancylostomiasis/drug therapy Cyclosporine/therapeutic use Glucocorticoids/therapeutic use Immunosuppressive Agents/therapeutic use Prednisolone/therapeutic use [Mh] MeSH terms secundary: Ancylostomiasis/immunology Animals Cell Proliferation/drug effects Cricetinae Cyclosporine/pharmacology Disease Models, Animal Feces/parasitology Female Glucocorticoids/pharmacology Immunoglobulin G/blood Immunosuppressive Agents/pharmacology Intestine, Small/parasitology Intestine, Small/pathology Lymph Nodes/cytology Lymph Nodes/drug effects Lymph Nodes/immunology Mesentery Mesocricetus Parasite Egg Count Prednisolone/pharmacology Spleen/pathology [Pt] Publication type: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Name of substance: 0 (Glucocorticoids); 0 (Immunoglobulin G); 0 (Immunosuppressive Agents); 50-24-8 (Prednisolone); 59865-13-3 (Cyclosporine) [Em] Entry month: 1302 [Js] Journal subset: IM [Da] Date of entry for processing: 121225 [St] Status: MEDLINE

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[PMID]: 22795675 [Au] Autor: Chung CS; Lin CK; Su KE; Liu CY; Lin CC; Liang CC; Lee TH [Ad] Address: Department of Internal Medicine, Far Eastern Memorial Hospital, Banciao, New Taipei City, Taiwan. [Ti] Title: Diagnosis of Ancylostoma ceylanicum infestation by single-balloon enteroscopy (with video). [So] Source: Gastrointest Endosc;76(3):671-2, 2012 Sep. [Is] ISSN: 1097-6779 [Cp] Country of publication: United States [La] Language: eng [Mh] MeSH terms primary: Ancylostomiasis/diagnosis Endoscopy, Gastrointestinal [Mh] MeSH terms secundary: Ancylostoma Ancylostomiasis/drug therapy Animals Antinematodal Agents/therapeutic use Female Humans Mebendazole/therapeutic use Middle Aged [Pt] Publication type: CASE REPORTS; JOURNAL ARTICLE; VIDEO-AUDIO MEDIA [Nm] Name of substance: 0 (Antinematodal Agents); 31431-39-7 (Mebendazole) [Em] Entry month: 1301 [Js] Journal subset: IM [Da] Date of entry for processing: 120817 [St] Status: MEDLINE [do] DOI: 10.1016/j.gie.2012.05.010

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[PMID]: 22394387 [Au] Autor: Liotta JL; Youn H; Aksel S; Bienhoff SE; Bowman DD [Ad] Address: Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401, USA. [Ti] Title: Prevalence of Ancylostoma braziliense in dogs from Alachua and Marion Counties, Florida, United States. [So] Source: J Parasitol;98(5):1039-40, 2012 Oct. [Is] ISSN: 1937-2345 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: A convenience collection of fecal samples from 148 dogs in northern Florida was examined for the presence of Ancylostoma braziliense eggs by using centrifugal sugar flotation and polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP). Of the 148 samples, 64 (43.2%) contained hookworm eggs. DNA from 42 samples was successfully amplified using PCR; using RFLP, 2 samples were identified as containing DNA of A. braziliense (4.8% of the 42 successfully amplified samples). [Mh] MeSH terms primary: Ancylostomiasis/veterinary Dog Diseases/epidemiology [Mh] MeSH terms secundary: Ancylostoma/genetics Ancylostoma/isolation & purification Ancylostomiasis/epidemiology Animals DNA, Helminth/chemistry DNA, Helminth/isolation & purification Dog Diseases/parasitology Dogs Feces/parasitology Florida/epidemiology Polymerase Chain Reaction/veterinary Polymorphism, Restriction Fragment Length Prevalence [Pt] Publication type: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Name of substance: 0 (DNA, Helminth) [Em] Entry month: 1212 [Js] Journal subset: IM [Da] Date of entry for processing: 121022 [St] Status: MEDLINE [do] DOI: 10.1645/GE-2927.1

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