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[PMID]: 29524493
[Au] Autor:Shali A; Hasannia S; Gashtasbi F; Masoud Abdous S; Shirin Shahangian S; Jalili S
[Ad] Address:Department of Biology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
[Ti] Title:Generation and screening of efficient neutralizing single domain antibodies (VHHs) against the critical functional domain of anthrax protective antigen (PA).
[So] Source:Int J Biol Macromol;, 2018 Mar 07.
[Is] ISSN:1879-0003
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Since anthrax is an acute infectious disease, detection and neutralization of the toxins of pathogenic Bacillus anthracis are of great importance. The critical role of protective antigen (PA) component of tripartite anthrax toxin in toxin entry into the host cell cytosol provided a great deal of effort to generate monoclonal antibodies against this constitute. Regarding the importance of anthrax detection/neutralization and unique physicochemical and pharmacological features of VHHs as single domain antibodies, the present study aimed to generate VHHs against the receptor binding domain of PA, termed PAD4. After camel immunization, a gene repertoire of VHH fragments with a diversity of 4.7 × 108 clones was produced, followed by constructing a VHH phage display library. A stringent successive biopanning was then carried out to isolate the phages displaying high affinity VHHs against PAD4.Polyclonal and monoclonal Enzyme-linked immunosorbent assay (ELISA) verified binding specificity of phages to the target protein. Modeling of VHHs together with the docking simulation studies, illustrated the binding site of antibodies on antigen. Docking analysis revealed that all selected VHHs potently cover the key functional residues of PAD4. Since the selected VHHs could cover and block the receptor binding loops of PA, they could be proposed as hopeful anti-Anthrax candidates.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

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[PMID]: 29499363
[Au] Autor:Chen S; Zhang N; Shao J; Wang X
[Ad] Address:Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, No. 100, Haining Road, Shanghai, 200080, China.
[Ti] Title:Maintenance versus non-maintenance intravesical Bacillus Calmette-Guerin instillation for non-muscle invasive bladder cancer: A systematic review and meta-analysis of randomized clinical trials.
[So] Source:Int J Surg;52:248-257, 2018 Feb 28.
[Is] ISSN:1743-9159
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: It is not clear whether maintenance Bacillus Calmette-Guerin (BCG) is necessary for intermediate- or high-risk non-muscle-invasive bladder cancer (NMIBC). This systematic review and meta-analysis aimed to illustrate the effects of maintenance BCG for intermediate- or high-risk NMIBC. METHODS: A comprehensive literature search of PubMed, EMBASE, Cochrane Library, ClinicalTrials.gov databases and International Clinical Trials Register (ICTRP) Search was conducted to identify relevant randomized controlled trials (RCTs) that have assessed the efficacy of maintenance or non-maintenance BCG therapy for patients with NMIBC. The maintenance group first received induction BCG instillations, and subsequently received BCG intravesical instillations regularly for at least 1 year, while the control group only received induction BCG instillations. Systematic review and meta-analysis were performed according to Preferred Reporting Items for Systematic Reviews and Meta-analysis Criteria. RESULTS: Ten RCTs were eligible in this systematic review. The meta-analysis showed that induction BCG followed by maintenance BCG instillation after transurethral resection (TUR) could reduce the risk ratios of tumor recurrence by 21% (RR = 0.79; 95% CI 0.70-0.89; P < 0.0001) and prolong recurrence-free survival (RFS) by 33% (HR: 0.67; 95% CI, 0.54-0.82; P < 0.001), compared with non-maintenance BCG. It could also reduce the risk ratios of tumor progression (RR = 0.81; 95% CI 0.68-0.97; P = 0.02). However, these pooled results should be considered with caution since the quality of evidences for outcomes ranged low. Subgroup analysis implied that different durations of maintenance BCG instillations might be one of the sources of potential clinical heterogeneity of included studies. Begg's funnel plot and Egger's test did not reveal any evidence of publication bias in this meta-analysis. CONCLUSIONS: Induction BCG followed by maintenance BCG instillation after TUR, compared with induction BCG along, can reduce the risk ratios of tumor recurrence and tumor progression, and prolong RFS. However, these results with a lower level of evidence should be treated with caution. The optimal maintenance schedule has yet to be determined and a large multi-institutional study in intermediate- and high-risk patients is also needed to determine the optimal maintenance schedule.
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

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[PMID]: 29486275
[Au] Autor:Salem MZM; Behiry SI; Salem AZM
[Ad] Address:Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, Egypt.
[Ti] Title:Effectiveness of root-bark extract from Salvadora persica against the growth of certain molecularly identified pathogenic bacteria.
[So] Source:Microb Pathog;117:320-326, 2018 Feb 24.
[Is] ISSN:1096-1208
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:The acetone extract from root-bark of Salvadora persica L. (Salvadoraceae), is assayed for its antibacterial activity against some bacterial pathogens. By GC/MS analysis, the main chemical components of the acetone extract were found to be benzylisothiocyanate (39.4%), and benzyl nitrile (benzeneacetonitrile) (37.9%). According the extract concentrations used, the measured inhibition zones observed were between from 13.6 to 18.6 mm, 15.3-23 mm, 13.3-18.3 mm, 13.3-18.3 mm, and 12.3-19 mm, against the isolated plant bacterial pathogens namely Agrobacterium tumefaciens, Pectobacterium atrosepticum, Enterobacter cloacae, Dickeya solani and Ralstonia solanacearum, respectively, whilst it was between 8 and 12 mm, 8-9.6 mm, 8-11.6 mm, and 8-10.3 mm against Bacillus subtilis, Sarcina lutea, Escherichia coli and Staphylococcus aureus, respectively. The minimum inhibitory concentration values of the extract were between 16 and 32 µg/mL against the growth of plant bacterial, and from 1000 to 2000 µg/mL against the growth of the human bacteria. In conclusion, the acetone extract of root-bark of S. persica showed strong antibacterial activity against the plant pathogens and some activity against the human pathogens were reported. The results suggested that using the acetone extract from root-bark of S. persica as bioactive agent against the growth of the studied plant bacterial pathogens.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

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[PMID]: 29474841
[Au] Autor:Villa-Rodríguez E; Ibarra-Gámez C; de Los Santos-Villalobos S
[Ad] Address:Instituto Tecnológico de Sonora, 5 de Febrero 818 Sur, C.P.85000, Col. Centro, Ciudad Obregón, Sonora, Mexico.
[Ti] Title:Extraction of high-quality RNA from Bacillus subtilis with a lysozyme pre-treatment followed by the Trizol method.
[So] Source:J Microbiol Methods;147:14-16, 2018 Feb 21.
[Is] ISSN:1872-8359
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:A suitable technique (lysozyme combined with Trizol reagent) was developed to improve the RNA yield, purity and integrity from Bacillus subtilis, under different bacterial growth stages. The obtained RNA was intact, having the required characteristics for downstream applications.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  5 / 118081 MEDLINE  
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[PMID]: 29462749
[Au] Autor:Gao X; Zhang M; Li X; Han Y; Wu F; Liu Y
[Ad] Address:Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.
[Ti] Title:Effects of a probiotic (Bacillus licheniformis) on the growth, immunity, and disease resistance of Haliotis discus hannai Ino.
[So] Source:Fish Shellfish Immunol;76:143-152, 2018 Feb 17.
[Is] ISSN:1095-9947
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:To study the effects of a probiotic (Bacillus lincheniformis) on the survival and growth of Haliotis discus hannai Ino, the expression levels of nonspecific immune genes and the resistance to Vibrio parahaemolyticus infection were assessed. Abalones (shell length: 27.64 ±â€¯1.59 mm, body weight: 4.17 ±â€¯0.32 g) were selected for use in an 8-week culture experiment and a 2-week V. parahaemolyticus artificial infection experiment. In both experiments, the control group (C) was fed with a basal feed and the experimental groups were fed with experimental food prepared by spraying the probiotic on the basal feed at different concentrations: 10 (B1), 10 (B2), and 10 (B3) cfu/mL. The survival rate, total number of blood lymphocytes, activity of acid phosphatase, and expression level of heat shock protein 70 were significantly higher in B1, B2, and B3 than in C (P < 0.05). The specific growth rate of shell length, food intake, food conversion rate, phagocytic activity of blood lymphocytes, activities of myeloperoxidase and catalase (CAT), and expression levels of CAT and thioredoxin peroxidase of abalones in B2 were significantly higher than those in B1 and C (P < 0.05). Although the level of O produced by the respiratory burst of blood lymphocytes in B2 was not significantly different from those in B1 and B3, they were significantly higher than that in C (P < 0.05). The activity of superoxide dismutase (SOD), the nitric oxide levels produced by the respiratory burst of blood lymphocytes, and the expression levels of Mn-SOD in B1 and B3 were significantly higher than those in C but significantly lower than those in B2 (P < 0.05). Fourteen days after infection with V. parahaemolyticus, the cumulative mortality of abalones in B2 was significantly lower than those in B1 and C (P < 0.05). These results indicate that the food containing 10 cfu/mL Bacillus licheniformis promoted food intake and growth of abalones and also improved their resistance to V. parahaemolyticus infection. Thus, B. licheniformis is a good potential probiotic.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  6 / 118081 MEDLINE  
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[PMID]: 29452187
[Au] Autor:Ijadi Bajestani M; Mousavi SM; Mousavi SB; Jafari A; Shojaosadati SA
[Ad] Address:Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, Tehran, Iran.
[Ti] Title:Purification of extra cellular poly-γ-glutamic acid as an antibacterial agent using anion exchange chromatography.
[So] Source:Int J Biol Macromol;113:142-149, 2018 Feb 13.
[Is] ISSN:1879-0003
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:BLAST analysis of the 16S rRNA gene sequence for the newly isolated bacterium, revealed significant identity (99.5%) with Bacillus sonorensis [Ijadi Bajestani, M., et al., International Journal of Biological Macromolecules, 2017. 96: p. 100-110]. According to the literature review for closely related species of Bacillus sonorensis, the production of poly-γ-glutamic acid (γ-PGA) as an extra cellular biopolymer was investigated for the isolated bacteria which is deposited in IBRC (Iranian Biological Resource Center) as Bacillus sp. Strain M2 (IBRC-M11173). To determine if γ-PGA production by Bacillus sp. Strain M2 is glutamate dependent, it was grown on PGA medium, consisted of sodium glutamate. The results proved that γ-PGA production is highly dependent on glutamate component. In the following, the bioproduct has undergone different purification processes mainly consisting of dialysis, deproteinization and anion exchange chromatography. Based on the high-performance liquid chromatography (HPLC) results for ion chromatography effluents, 59% of the initial PGA in main solution was eluted via NaCl elution. Gel permeation chromatography (GPC) characterization analysis was accomplished to determine the polydispersity and γ-PGA molecular weight. Two major average molecular weights were distinguished; the heavy weight fraction of 7.7×10 g/mol with polydispersity index of 1.73 and the other one with an average molecular weight number of 1.7×10 g/mol and polydispersity index of 4.4. The antibacterial activity of the extracellular γ-PGA, as an anionic biopolymer, toward Staphylococcus aureus and E. coli, was assayed using the clinical and laboratory standards institute (CLSI) guidelines. For Staphylococcus aureus the minimum inhibitory concentration (MIC) value was about 34g/L while for E. coli this value reaches 53g/L.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  7 / 118081 MEDLINE  
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[PMID]: 29432912
[Au] Autor:Abuelizz HA; El-Dib RA; Marzouk M; Al-Salahi R
[Ad] Address:Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
[Ti] Title:In vitro evaluation of new 2-phenoxy-benzo[g][1,2,4]triazolo[1,5-a]quinazoline derivatives as antimicrobial agents.
[So] Source:Microb Pathog;117:60-67, 2018 Feb 09.
[Is] ISSN:1096-1208
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Previously, seventeen 2-phenoxy-benzo[g][1,2,4]triazolo[1,5-a]quinazoline derivatives were prepared and characterized by physicochemical and spectral means. This study was conducted to evaluate their activities in vitro against five Gram-negative and five Gram-positive of clinically pathogenic bacterial strains and ten fungal strains. The antimicrobial activity was assessed, and the minimum inhibitory concentration values of the tested compounds were determined in µg ml , using the diffusion agar technique. The bacterial strains used were Escherichia coli (ATCC 25922), Proteus mirabilis (ATCC 7002), Klebsiella oxytoca (ATCC 700324), Pseudomonas aeruginosa (ATCC 10145), Enterobacter cloacae (ATCC 13047D-5), Bacillus subtilis (NRRL B-543), Enterococcus faecalis (RCMB 0100154-2), Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 12228), and Streptococcus pyogenes (RCMB 0100174-2). Aspergillus fumigatus (RCMB 02568), Syncephalastrum racemosum (IMI 21178), Geotricum candidum (IMI 329542), Candida albicans (ATCC 10231), Aspergillus niger (IMI 130783), Cryptococcus neoformans (NRRL Y-1518), Candida tropicalis (RCMB 05239), Penicillium expansum (IMI 146655), Microsporum canis (RCMB 0834), and Trichophyton mentagrophytes (RCMB 0925) were used as the fungal strains. Ampicillin and gentamicin were used as reference antibacterial drugs and amphotericin B was used as the reference antifungal drug. The antimicrobial studies revealed that the tested compounds 6-8, 11, 12, and 14-16 showed the highest activities against the bacterial and fungal strains. The current study showed that some benzo[g]traizoloquinazolines displayed remarkable antimicrobial activity and could be used as template for further design of potent antimicrobial agent.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  8 / 118081 MEDLINE  
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[PMID]: 29428423
[Au] Autor:Ait Bahadou S; Ouijja A; Karfach A; Tahiri A; Lahlali R
[Ad] Address:Phytopathology Unit, Department of Plant Protection and Environment, Ecole Nationale d'Agriculture de Meknès, Km10, Rte Haj Kaddour, BP S/40, Meknès 50001, Morocco; Laboratory of Plant Biotechnology and Molecular Biology, Moulay Ismail University, Faculty of Sciences, BP 11201, Ave Zitoune, Meknes,
[Ti] Title:New potential bacterial antagonists for the biocontrol of fire blight disease (Erwinia amylovora) in Morocco.
[So] Source:Microb Pathog;117:7-15, 2018 Feb 08.
[Is] ISSN:1096-1208
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:The effectiveness of antagonistic bacteria to control Erwinia amylovora was evaluated under in vitro and field conditions. Among 61 bacteria isolated from soil and flowers of fire blight host plants of different Moroccan areas, 20 bacterial isolates showed higher antagonistic activity against the pathogen during agar-diffusion-test, attached blossoms assay and in a bioassay on immature pear fruits. Effective isolates were identified by using biochemical tests and 16 S rRNA genes sequencing. These isolates were grouped into the following genera: Alcaligenes (ACBC1), Pantoea (ACBC2, ACBP1, and ACBP2), Serratia (HC4), Brevibacterium (SF3, SF4, SF7, and SF15), Pseudomonas (SP9), and Bacillus (CPa12, CPa2, HF6, JB2, LMR2, SF14, SF16, SP10, SP13, and SP18). Furthermore, isolates were reported in the NCBI nucleotide sequence database (Genbank) under the accession numbers from KY357285 to KY357304. A 2-year field trials consisted of spray treatments with different bacterial antagonists was conducted on the susceptible apple cultivars 'Gala', 'Golden Parsi' and 'Golden Smoothee'. Their efficacies were evaluated 15 days post-inoculation on detached blossoms and were ranged from 54.6 to 95.0% for 11 strains, most of them were slightly better or better than that obtained with commercial bacterial strains P10c (66%) and QST713 (63%). In field trials, the most effective were P. agglomerans ACBP2, B. amyloliquefaciens LMR2, B. halotolerans (SF3 and SF4), and B. mojarvensis SF16. In addition, effective bacterial isolates did not show the pathogenicity signs towards plant tissue and are, therefore, considered as potential candidates to be integrated in actives ingredients of microbial formulation for the effective control of Fire Blight.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  9 / 118081 MEDLINE  
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[PMID]: 29421667
[Au] Autor:Boulton ML; Carlson BF; Power LE; Wagner AL
[Ad] Address:Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI, USA; Department of Internal Medicine, Division of Infectious Diseases, University of Michigan Medical School, Ann Arbor, MI, USA. Electronic address: mboulton@umich.edu.
[Ti] Title:Socioeconomic factors associated with full childhood vaccination in Bangladesh, 2014.
[So] Source:Int J Infect Dis;69:35-40, 2018 Feb 05.
[Is] ISSN:1878-3511
[Cp] Country of publication:Canada
[La] Language:eng
[Ab] Abstract:OBJECTIVES: Childhood vaccination in Bangladesh has improved, but there is room for improvement. This study estimated full immunization coverage in Bangladeshi children and characterized risk factors for incomplete immunization. METHODS: Using the 2014 Bangladesh Demographic and Health Survey (DHS), full vaccination of children aged 12 to 24 months was examined; this was defined as the receipt of one dose of bacillus Calmette-Guérin (BCG), three doses of pentavalent vaccine, three doses of oral polio vaccine (OPV), and one dose of measles-containing vaccine (MCV). Associations between full vaccination and selected risk factors were assessed by logistic regression. RESULTS: Overall, 83% of children were fully vaccinated. BCG had the highest completion (97%), followed by OPV (92%), pentavalent vaccine (91%), and MCV (85%). Full vaccination coverage ranged from 64.4% in Sylhet to 90.0% in Rangpur and was lowest among non-locals of all regions (78.4%). Children who were in the lowest wealth quintile, who had mothers without antenatal care visits, or who had mothers without autonomy in healthcare decision-making were less likely to be fully vaccinated. CONCLUSIONS: Overall, full vaccination of children is high, but varies by vaccine type. Disparities still exist by wealth and by region. Maternal access to care and autonomy in healthcare decision-making are associated with higher vaccination coverage.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher

  10 / 118081 MEDLINE  
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[PMID]: 29524170
[Au] Autor:Matovic K; Ciric J; Kaljevic V; Nedic N; Jevtic G; Vaskovic N; Baltic MZ
[Ad] Address:Veterinary Specialized Institute Kraljevo, 34 Zicka Street, Kraljevo, 36000, Serbia.
[Ti] Title:Physicochemical parameters and microbiological status of honey produced in an urban environment in Serbia.
[So] Source:Environ Sci Pollut Res Int;, 2018 Mar 09.
[Is] ISSN:1614-7499
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Honey is a natural substance produced by honey bees (the genus Apis) enjoyed by people due to its unique nutritional and medicinal properties. The aim of this study was to determine the physicochemical parameters (moisture, ash, water-insoluble content, reducing sugars, sucrose, free acidity, diastase activity, hydroxymethylfurfural, and electrical conductivity) and microbiological status (total number of aerobic mesophilic bacteria, total number of sulfite-reducing clostridia, the presence of Salmonella spp., total numbers of fungi and yeasts and the presence of Clostridium botulinum) in honey (honeydew, blossom, sunflower, acacia, and linden) produced in an urban environment in Serbia. We analyzed 19 apiary samples of honey, collected during the 2011 harvesting season, by using recommendation methods. Physicochemical parameters of the examined honey produced in the urban environment indicated the honeys were of acceptable quality. Bacillus spp. were detected in four honeys, yeasts were detected in three honeys, and Clostridium botulinum type E was detected in one honey using PCR. The current study also showed the presence of diverse honey varieties in Serbia.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:Publisher
[do] DOI:10.1007/s11356-018-1659-1


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