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[PMID]: 29522515
[Au] Autor:Prieto-Torres DA; Cuervo AM; Bonaccorso E
[Ad] Address:Master Oficial en Biodiversidad en Áreas Tropicales y su Conservación. Universidad Internacional Menéndez Pelayo, Madrid, España.
[Ti] Title:On geographic barriers and Pleistocene glaciations: Tracing the diversification of the Russet-crowned Warbler (Myiothlypis coronata) along the Andes.
[So] Source:PLoS One;13(3):e0191598, 2018.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:We studied the phylogeography and plumage variation of the Russet-crowned Warbler (Myiothlypis coronata), from Venezuela to Bolivia, with focus on populations from Ecuador and northern Peru. We analyzed sequences of mitochondrial and nuclear genes, geographic distributions, as well as photographs of specimens deposited at museum collections. Phylogenetic analyses identified three major lineages formed by populations from: Venezuela and Colombia (M. c. regulus), Ecuador and northern Peru (M. elata, M. castaneiceps, M. orientalis, M. c. chapmani), and central Peru and Bolivia (M. c. coronata). We found further population structure within M. c. regulus and M. c. coronata, and population structure and complexity of plumage variation within the Ecuador-northern Peru lineage. Time-calibrated trees estimated that most intraspecific variation originated during the Pleistocene; however, this pattern may not be attributed to an increase in diversification rate during that period. We discuss these results in the context of the importance of geographic-ecological barriers in promoting lineage diversification along the Andes and put forward a preliminary taxonomic proposal for major lineages identified in this study.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:In-Data-Review
[do] DOI:10.1371/journal.pone.0191598

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[PMID]: 29451846
[Au] Autor:Briosio-Aguilar R; Pinto HA; Rodríguez-Santiago MA; López-García K; Garcia-Varela M; Perez-Ponce de Leon G
[Ad] Address:* Instituto de Biologia. UNAM.
[Ti] Title:Link Between the Adult and the Metacercaria of Clinostomum heluans Braun, 1899 (Trematoda: Clinostomidae) Through DNA Sequences, and its Phylogenetic Position within the Genus Clinostomum Leidy, 1856.
[So] Source:J Parasitol;, 2018 Mar 09.
[Is] ISSN:1937-2345
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The phylogenetic position of Clinostomum heluans Braun, 1899 within the genus Clinostomum Leidy, 1856 is reported in this study based on sequences of the barcoding region of the mitochondrial cytochrome c oxidase subunit 1 gene (COX1). Additionally, molecular data are used to link the adult and the metacercariae of the species. The metacercariae of C. heluans were found encysted infecting the cichlid fish Australoheros sp. in Minas Gerais, Brazil, whereas the adults were obtained from the mouth cavity of the Great White egret Ardea alba, in Campeche, Mexico. The COX1sequences obtained for the Mexican clinostomes and the Brazilian metacercaria were almost identical (0.2% of molecular divergence), indicating conspecificity. Similar molecular divergence (0.2-0.4%) were found between sequences of C. heluans here reported and Clinostomum sp. 6 (after Locke et al., 2015) previously obtained from a metacercaria recovered from the cichlid Cichlasoma boliviense, in Santa Cruz, Bolivia. Both, Maximum Likelihood (ML) and Bayesian Inference (BI) analyses unequivocally show the conspecificity between C. heluans and Clinostomum sp. 6, which form a monophyletic clade with high nodal support and very low genetic divergence. Moreover, tree topology reveals that C. heluans occupies a basal position with respect to New World species of Clinostomum, although a more dense taxon sampling of species within the genus is further required. The metacercaria of C. heluans seems to be specific to cichlid fish because both samples from South America were recovered from species of this fish family, although not closely related.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:Publisher
[do] DOI:10.1645/17-183

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[PMID]: 29415037
[Au] Autor:Abbott P; Banerjee T; Aruquipa Yujra AC; Xie B; Piette J
[Ad] Address:University of Michigan School of Nursing; Ann Arbor, Michigan, United States of America.
[Ti] Title:Exploring chronic disease in Bolivia: A cross-sectional study in La Paz.
[So] Source:PLoS One;13(2):e0189218, 2018.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PURPOSE: This study seeks to develop an understanding that can guide development of programs to improve health and care for individuals with Non-Communicable Diseases (NCDs) in La Paz, Bolivia, where NCDs are prevalent and primary care systems are weak. This exploratory investigation examines the characteristics of chronic disease patients in the region, key health related behaviors, and their perceptions of the care that they receive. The longer-term goal is to lay groundwork for interventional studies based on the principles of the Chronic Care Model (CCM). SUBJECTS AND METHODS: The study is based on two surveys of adults (> 18 years old) administered in 2014 in La Paz, Bolivia. A total of 1165 adult patients participated in the first screening survey. A post-screening second survey, administered only on those who qualified based on Survey 1, collected more detailed information about the subjects' general health and health related personal circumstances, several health behaviors, health literacy, and their perceptions of care received. A final data set of 651 merged records were used for analysis. RESULTS: Characteristic of a low-income country, the majority of participants had low levels of education, income, health literacy and high rates of under/unemployment. Nearly 50% of participants reported 2 or more NCDs. Seventy-four percent (74%) of respondents reported low levels of medication adherence and 26% of the population was found to have an undiagnosed depressive disorder. Overall, the perception of care quality was low (60%), particularly in those under the age of 45. Significant relationships emerged between several sociodemographic characteristics, health behaviors, and perceptions that have major implications for improving NCD care in this population. CONCLUSIONS: These findings illustrate some of the challenges facing low-income countries where reversing the tide of NCDs is of great importance. The prevalence of NCDs coupled with challenging social determinants of health, poor medication adherence, low health literacy, and perceptions of low quality of healthcare highlight several areas of opportunity for intervention.
[Mh] MeSH terms primary: Chronic Disease/epidemiology
[Mh] MeSH terms secundary: Aged
Bolivia/epidemiology
Cross-Sectional Studies
Female
Humans
Logistic Models
Male
Middle Aged
Patient Compliance
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[Js] Journal subset:IM
[Da] Date of entry for processing:180208
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189218

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[PMID]: 29391199
[Au] Autor:Arévalo-Lopéz D; Nina N; Ticona JC; Limachi I; Salamanca E; Udaeta E; Paredes C; Espinoza B; Serato A; Garnica D; Limachi A; Coaquira D; Salazar S; Flores N; Sterner O; Giménez A
[Ad] Address:Instituto de Investigaciones Fármaco-Bioquímicas (IIFB), Facultad de Ciencias Farmacéuticas y Bioquímicas, Universidad Mayor de San Andrés (UMSA), Av. Saavedra No 2224, Miraflores, La Paz, Bolivia.
[Ti] Title:Leishmanicidal and cytotoxic activity from plants used in Tacana traditional medicine (Bolivia).
[So] Source:J Ethnopharmacol;216:120-133, 2018 Apr 24.
[Is] ISSN:1872-7573
[Cp] Country of publication:Ireland
[La] Language:eng
[Ab] Abstract:ETNOPHARMACOLOGICAL RELEVANCE: Thirty-eight Tacana medicinal plant species used to treat skin problems, including leishmania ulcers, skin infections, inflammation and wound healing, were collected in the community of Buena Vista, Bolivia, with the Tacana people. Twenty two species are documented for the first time as medicinal plants for this ethnic group living in the northern area of the Department of La Paz. AIM OF THE STUDY: To evaluate the leishmanicidal effect (IC ) and cytotoxicity (LD ) of the selected plants. To carry out bioguided studies on the active extracts. To assess the potential of Bolivian plant biodiversity associated with traditional knowledge in the discovery of alternative sources to fight leishmaniasis. MATERIALS AND METHODS: Seventy three ethanol extracts were prepared from 38 species by maceration and were evaluated in vitro against promastigotes of Leishmania amazonensis and L. braziliensis. Active extracts (IC ≤ 50 µg/mL) were fractionated by chromatography on Silica gel column and the fractions were assessed against the two Leishmania strains. The most active fractions and the crude extracts were evaluated against reference strains of L. amazonensis, L. braziliensis, L. aethiopica, two native strains (L. Lainsoni and L. braziliensis) and for cytotoxicity against HeLa cells. The chromatographic profile of the active fractions was obtained by reverse phase chromatography using HPLC. RESULTS: From the 73 extracts, 39 extracts (53.4%) were inactive and 34 showed activity. Thirteen species were sselected for bioguided studies. The crude extracts and their 36 fractions were evaluated against two Leishmania strains. The most active fraction were tested in a panel of five leishmania strains and for cytotoxicity. The Selective Index (SI = LD /IC ) was calculated, and were generally low. Retention time and UV spectra were recorded for the active fractions by HPLC-DAD using a reverse phase column. Profiles were very different from each other, showing the presence of different compounds. CONCLUSION: Bolivian traditional knowledge from the Tacanba was useful to identify plants with effect on Leishmania promastigotes. Chromatographic bioguided studies showed stronger leishmanicidal and cytotoxic activity for the medium polar fraction. HPLC analysis showed different chromatographic profiles of the active fractions.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180310
[Lr] Last revision date:180310
[St] Status:In-Process

  5 / 3377 MEDLINE  
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[PMID]: 29252210
[Au] Autor:Lange R; Martinez-Garrido C; Ventura A
[Ad] Address:Rense Lange, 107 Carefree Dr., Chatham, IL 62629, USA, Rense.Lange@gmail.com.
[Ti] Title:I'm scared to go to School! Capturing the Effects of Chronic Daily Fears on Students' Concept of Self.
[So] Source:J Appl Meas;18(4):420-433, 2017.
[Is] ISSN:1529-7713
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Students may experience considerable fear and stress in school settings, and based on Dweck's (2006) notion of "mindset" we hypothesized that fear introduces qualitative changes in students' self-concepts. Hypotheses were tested on 3847 third-grade students from nine Iberoamerican countries (Bolivia, Chile, Colombia, Cuba, Ecuador, Panama, Peru, Spain, and Venezuela), who completed Murillo's (2007) adaptation of Marsh' (1988) SDQ-I. Rasch scaling indicated that the information-content of High-Fear students' ratings was more localized across the latent dimension than was that of Low-Fear students, and their ratings also showed less cognitive variety. The resulting measurement distortions could be captured via logistic regression over the ratings' residuals. Also, using training and validation samples (with respectively 60 and 40% of all cases), it proved possible to predict students' fear levels and their gender. We see the present findings as a first step towards implementing an online warning and detection system for signs of bullying among students.
[Mh] MeSH terms primary: Anxiety/epidemiology
Anxiety/psychology
Data Interpretation, Statistical
Psychometrics/methods
Students/psychology
Students/statistics & numerical data
Surveys and Questionnaires
[Mh] MeSH terms secundary: Adolescent
Algorithms
Anxiety/diagnosis
Child
Fear/psychology
Female
Humans
Male
Models, Statistical
Reproducibility of Results
Sensitivity and Specificity
South America/epidemiology
Young Adult
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[Js] Journal subset:IM
[Da] Date of entry for processing:171219
[St] Status:MEDLINE

  6 / 3377 MEDLINE  
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[PMID]: 29513667
[Au] Autor:Saba Villarroel PM; Nurtop E; Pastorino B; Roca Y; Drexler JF; Gallian P; Jaenisch T; Leparc-Goffart I; Priet S; Ninove L; de Lamballerie X
[Ad] Address:UMR EPV Émergence des Pathologies Virales, Aix-Marseille University-IRD 190-Inserm 1207 -EHESP-IHU Méditerranée Infection, Marseille, France.
[Ti] Title:Zika virus epidemiology in Bolivia: A seroprevalence study in volunteer blood donors.
[So] Source:PLoS Negl Trop Dis;12(3):e0006239, 2018 Mar 07.
[Is] ISSN:1935-2735
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Zika virus (ZIKV), was widely reported in Latin America and has been associated with neuropathologies, as microcephaly, but only few seroprevalence studies have been published to date. Our objective was to determine the seroprevalence amongst Bolivian blood donors and estimate the future potential circulation of the virus. METHODOLOGY: A ZIKV seroprevalence study was conducted between December 2016 and April 2017 in 814 asymptomatic Bolivian volunteer blood donors residing in various eco-environments corresponding to contrasting entomological activities. It was based on detection of IgG to ZIKV using NS1 ELISA screening, followed by a seroneutralisation test in case of positive or equivocal ELISA result. CONCLUSIONS/SIGNIFICANCE: Analysis revealed that ZIKV circulation occurred in tropical areas (Beni: 39%; Santa Cruz de la Sierra: 21.5%) but not in highlands (~0% in Cochabamba, La Paz, Tarija). It was modulated by Aedes aegypti activity and the virus spread was not limited by previous immunity to dengue. Cases were geo-localised in a wide range of urban areas in Santa Cruz and Trinidad. No differences in seroprevalence related to gender or age-groups could be identified. It is concluded that ZIKV has been intensely circulating in the Beni region and has still a significant potential for propagating in the area of Santa Cruz.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[St] Status:Publisher
[do] DOI:10.1371/journal.pntd.0006239

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[PMID]: 29438471
[Au] Autor:Bhattacharyya T; Messenger LA; Bern C; Mertens P; Gilleman Q; Zeippen N; Bremer Hinckel BC; Murphy N; Gilman RH; Miles MA; Chagas Working Group in Bolivia and Peru
[Ad] Address:Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.
[Ti] Title:Severity of Chagasic Cardiomyopathy Is Associated With Response To A Novel Rapid Diagnostic Test For Trypanosoma cruzi TcII/V/VI.
[So] Source:Clin Infect Dis;, 2018 Feb 09.
[Is] ISSN:1537-6591
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Background: Trypanosoma cruzi causes Chagas disease in the Americas. Outcome of infection ranges from lifelong asymptomatic status to severe disease. Understanding how history of T. cruzi lineage (TcI-TcVI) infection relates to clinical prognosis is challenging. We previously described peptide-based lineage-specific ELISA with Trypomastigote Small Surface Antigen (TSSA). Methods: A novel rapid diagnostic test (Chagas Sero K-SeT) incorporating a peptide corresponding to the TSSA-II/V/VI common epitope was developed, and validated by comparison with ELISA. Patients from Bolivia and Peru were then tested by Chagas Sero K-SeT, including individuals with varying cardiac pathology, and matched mothers and neonates. Results: Chagas Sero K-SeT and ELISAs, with a Bolivian subset of cardiac patients, mothers and neonates, were in accord. In adult chronic infections (n = 121), comparison of severity class A (no evidence of Chagas cardiomyopathy) against classes B (ECG suggestive of Chagas cardiomyopathy) and C/D (moderate/severe Chagas cardiomyopathy) revealed statistically significant increase in Chagas Sero K-SeT reactivity with increasing severity (Chi Square for trend 7.39; p = 0.007). In Peru, where TcII/V/VI lineages are rarely reported, Chagas Sero K SeT detected sporadic infections. Conclusions: We develop a novel, low-cost, point-of-care, rapid test and demonstrate that it can replace ELISA for identification of lineage-specific TSSA II/V/VI IgG. Most importantly, we show that response to the TSSA II/V/VI epitope in this RDT is associated with severity of Chagas cardiomyopathy, and thus may have prognostic value. Repeated challenge with T. cruzi infection may both exacerbate disease progression and boost the immune response to the TSSApep-II/V/VI epitope.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[St] Status:Publisher
[do] DOI:10.1093/cid/ciy121

  8 / 3377 MEDLINE  
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[PMID]: 29405102
[Au] Autor:Salvador F; Sánchez-Montalvá A; Sulleiro E; Moreso F; Berastegui C; Caralt M; Pinazo MJ; Moure Z; Los-Arcos I; Len O; Gavaldà J; Molina I
[Ad] Address:Department of Infectious Diseases, Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, PROSICS Barcelona, Barcelona, Spain.
[Ti] Title:Prevalence of Chagas Disease among Solid Organ-Transplanted Patients in a Nonendemic Country.
[So] Source:Am J Trop Med Hyg;98(3):742-746, 2018 Mar.
[Is] ISSN:1476-1645
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Reactivation of Chagas disease in the chronic phase may occur after solid organ transplantation, which may result in high parasitemia and severe clinical manifestations such as myocarditis and meningoencephalitis. The aim of the present study is to describe the prevalence of Chagas disease among solid organ-transplanted patients in a tertiary hospital from a nonendemic country. A cross-sectional study was performed at Vall d'Hebron University Hospital (Barcelona, Spain) from April to September 2016. Chagas disease screening was performed through serological tests in adult patients coming from endemic areas that had received solid organ transplantation and were being controlled in our hospital during the study period. Overall, 42 patients were included, 20 (47.6%) were male and median age was 50.5 (23-73) years. Transplanted organs were as follows: 18 kidneys, 17 lungs, and 7 livers. Three patients had Chagas disease, corresponding to a prevalence among this group of solid organ-transplanted patients of 7.1%. All three patients were born in Bolivia, had been diagnosed with Chagas disease and received specific treatment before the organ transplantation. We highly recommend providing screening tests for Chagas disease in patients with or candidates for solid organ transplantation coming from endemic areas, early treatment with benznidazole, and close follow-up to prevent clinical reactivations.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[St] Status:In-Data-Review
[do] DOI:10.4269/ajtmh.17-0735

  9 / 3377 MEDLINE  
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[PMID]: 29338137
[Au] Autor:Déniz FP; Encinas C; Fuente J
[Ad] Address:Embriovid, La Paz, Bolivia.
[Ti] Title:Morphological embryo selection: an elective single embryo transfer proposal.
[So] Source:JBRA Assist Reprod;22(1):20-25, 2018 Mar 01.
[Is] ISSN:1518-0557
[Cp] Country of publication:Brazil
[La] Language:eng
[Ab] Abstract:OBJECTIVE: To describe a patient selection method for elective single embryo transfer (eSET), emphasizing inclusion criteria and results. METHODS: This retrospective study included all cases seen in a private clinic between June 2011 and December 2016, in La Paz, Bolivia (3600 meters above sea level). Elective single embryo transfer was the method of choice in 34 IVF/ICSI cycles, all in the blastocyst stage. Gardner's blastocyst classification criteria were used. Between the two stages of the study (July 2015), each embryo grade implantation rate was recalculated, which led to the expansion of the inclusion criteria. RESULTS: The clinical pregnancy rate of the 34 cases in the first transfer group was 55.9% (19/34). Twin or multiple pregnancies did not occur. The cumulative pregnancy rate to date is 64% [(19+3)/34]. The first stage comprised 2.56% (12/468) of the patients offered elective single embryo transfers; the implantation rate was 58.3% (7/12). In the second stage, 14.29% (22/154) of the patients were eligible, and the implantation rate was 54.55% (12/22). CONCLUSIONS: The implementation of an eSET program based on in-depth morphological embryo assessment combined with the calculation of the implantation potential of each embryo grade led to acceptable clinical outcomes and fewer multiple pregnancies in patients transferred two embryos. Each clinic should be aware of the implantation rates of each embryo grade in its own setting.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[St] Status:In-Data-Review
[do] DOI:10.5935/1518-0557.20180015

  10 / 3377 MEDLINE  
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[PMID]: 28453779
[Au] Autor:Borges DA; Molina SMG; Pinto MC; Galati EAB; Cesario M; Ortiz DGS
[Ad] Address:Department of Genetics, Luiz de Queiroz College of Agriculture (ESALQ) - University of São Paulo (USP), Piracicaba, São Paulo, Brazil.
[Ti] Title:First Record of Lutzomyia (Lutzomyia) longipalpis (Diptera: Psychodidae: Phlebotominae) on the Trinational Frontier (Brazil-Peru-Bolivia) of South-Western Amazonia.
[So] Source:J Med Entomol;54(5):1425-1429, 2017 Sep 01.
[Is] ISSN:1938-2928
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:In South America, the main sand fly species involved in the transmission of Leishmania infantum chagasi (Cunha & Chagas, 1937), etiological agent of the visceral leishmaniasis (VL), is Lutzomyia longipalpis (Lutz & Neiva, 1912). The species has been recorded in Colombia, Venezuela, Bolivia, Argentina, Paraguay, Uruguay, and Brazil, where it is recorded in 24 of the 27 Brazilian states, except Acre, Amazonas, and Santa Catarina. Collections were carried out for one year (April 2013 to March 2014) using modified CDC light traps in different environments in Assis Brasil municipality, state of Acre. Two males of Lu. longipalpis were found in peridomiciliary location in a peri-urban area. This is the first record of the species in Acre. This finding may be considered by the health agencies located in the trinational frontier, and new collections are needed to evaluate the real distribution of the species.
[Mh] MeSH terms primary: Animal Distribution
Insect Vectors/physiology
Psychodidae/physiology
[Mh] MeSH terms secundary: Animals
Brazil
Leishmania infantum/physiology
Leishmaniasis, Visceral/transmission
Male
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1709
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[Js] Journal subset:IM
[Da] Date of entry for processing:170429
[St] Status:MEDLINE
[do] DOI:10.1093/jme/tjx086


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