Database : MEDLINE
Search on : choriocarcinoma [Words]
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[PMID]: 29463805
[Au] Autor:Ausman J; Abbade J; Ermini L; Farrell A; Tagliaferro A; Post M; Caniggia I
[Ad] Address:Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, ON, M5T 1X5, Canada.
[Ti] Title:Ceramide-induced BOK promotes mitochondrial fission in preeclampsia.
[So] Source:Cell Death Dis;9(3):298, 2018 Feb 20.
[Is] ISSN:2041-4889
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Mitochondria are in a constant balance of fusing and dividing in response to cellular cues. Fusion creates healthy mitochondria, whereas fission results in removal of non-functional organelles. Changes in mitochondrial dynamics typify several human diseases. However, the contribution of mitochondrial dynamics to preeclampsia, a hypertensive disorder of pregnancy characterized by placental cell autophagy and death, remains unknown. Herein, we show that the mitochondrial dynamic balance in preeclamptic placentae is tilted toward fission (increased DRP1 expression/activation and decreased OPA1 expression). Increased phosphorylation of DRP1 (p-DRP1) in mitochondrial isolates from preeclamptic placentae and transmission electron microscopy corroborated augmented mitochondrial fragmentation in cytotrophoblast cells of PE placentae. Increased fission was accompanied by build-up of ceramides (CERs) in mitochondria from preeclamptic placentae relative to controls. Treatment of human choriocarcinoma JEG3 cells and primary isolated cytrophoblast cells with CER 16:0 enhanced mitochondrial fission. Loss- and gain-of-function experiments showed that Bcl-2 member BOK, whose expression is increased by CER, positively regulated p-DRP1/DRP1 and MFN2 expression, and localized mitochondrial fission events to the ER/MAM compartments. We also identified that the BH3 and transmembrane domains of BOK were vital for BOK regulation of fission. Moreover, we found that full-length PTEN-induced putative kinase 1 (PINK1) and Parkin, were elevated in mitochondria from PE placentae, implicating mitophagy as the process that degrades excess mitochondria fragments produced from CER/BOK-induced fission in preeclampsia. In summary, our study uncovered a novel CER/BOK-induced regulation of mitochondrial fission and its functional consequence for heightened trophoblast cell autophagy in preeclampsia.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:In-Data-Review
[do] DOI:10.1038/s41419-018-0360-0

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[PMID]: 29436666
[Au] Autor:Zheng W; Liu T; Sun R; Yang L; An R; Xue Y
[Ad] Address:Department of Gynecology and Obstetrics, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
[Ti] Title:Daidzein induces choriocarcinoma cell apoptosis in a dose-dependent manner via the mitochondrial apoptotic pathway.
[So] Source:Mol Med Rep;17(4):6093-6099, 2018 Apr.
[Is] ISSN:1791-3004
[Cp] Country of publication:Greece
[La] Language:eng
[Ab] Abstract:Choriocarcinoma is a malignant gestational trophoblastic disease and relapse or drug resistance occurs in ~25% of gestational trophoblastic tumors. Cell apoptosis serves a role in the progression from hydatidiform mole to persistent gestational trophoblastic disease. It has been demonstrated that daidzein [7­hydroxy­3­(4­hydroxyphenyl)­4H­chromen­4­one] may induce apoptosis in a number of cancer types via the mitochondrial apoptotic pathway by altering the B­cell lymphoma (Bcl)­2/Bcl­2 associated X, apoptosis regulator (Bax) ratio, and activating the caspase cascade. Daidzein also serves a role in regulation of production of human chorionic gonadotropin in trophoblast cells and inhibition of cell proliferation. However, few reports have been published regarding the effect of daidzein on apoptosis in choriocarcinoma. Therefore, in the present study, JAR and JEG­3 human gestational choriocarcinoma cells were used to investigate the effect of daidzein on apoptosis of choriocarcinoma cells. Treatment with daidzein for 48 h reduced cell viability in a dose­dependent manner. The percentages of early and late apoptotic cells also increased following treatment with daidzein in a dose­dependent manner, with the number of late apoptotic cells increasing more prominently. Furthermore, treatment with daidzein led to apoptosis­associated alterations in nuclear morphology of JAR and JEG-3 cells. Expression levels of cleaved poly(ADP­ribose) polymerase, cleaved caspase­3 and cleaved caspase­9 increased following treatment with daidzein, whereas the Bcl­2/Bax ratio decreased in a dose­dependent manner. In conclusion, the results of the present study demonstrate that daidzein may induce apoptosis of choriocarcinoma cells in a dose­dependent manner via the mitochondrial apoptotic pathway.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[St] Status:In-Process
[do] DOI:10.3892/mmr.2018.8604

  3 / 7889 MEDLINE  
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[PMID]: 29483210
[Au] Autor:Chen KS; Fustino NJ; Shukla AA; Stroup EK; Budhipramono A; Ateek C; Stuart SH; Yamaguchi K; Kapur P; Frazier AL; Lum L; Looijenga LHJ; Laetsch TW; Rakheja D; Amatruda JF
[Ad] Address:Pediatrics, University of Texas Southwestern Medical Center.
[Ti] Title:EGF Receptor and mTORC1 are novel therapeutic targets in nonseminomatous germ cell tumors.
[So] Source:Mol Cancer Ther;, 2018 Feb 26.
[Is] ISSN:1538-8514
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Germ cell tumors (GCTs) are malignant tumors that arise from pluripotent embryonic germ cells and occur in children and young adults. GCTs are treated with cisplatin-based regimens which, while overall effective, fail to cure all patients and cause significant adverse late effects. The seminoma and non-seminoma forms of GCT exhibit distinct differentiation states, clinical behavior and response to treatment, however the molecular mechanisms of GCT differentiation are not fully understood. We tested whether the activity of the mammalian target of rapamycin complex 1 (mTORC1) and mitogen-activated protein kinase (MAPK) pathways were differentially active in the two classes of GCT. Here we show that non-seminomatous germ cell tumors (NSGCTs, including embryonal carcinoma, yolk sac tumor and choriocarcinoma) from both children and adults display activation of the mTORC1 pathway, while seminomas do not. In seminomas, high levels of REDD1 may negatively regulate mTORC1 activity. In NSGCTs, on the other hand, EGF and FGF2 ligands can stimulate mTORC1 and MAPK signaling, and members of the EGF and FGF receptor families are more highly expressed. Lastly, proliferation of NSGCT cells in vitro and in vivo is significantly inhibited by combined treatment with the clinically available agents erlotinib and rapamycin, which target EGFR and mTORC1 signaling, respectively. These results provide an understanding of the signaling network that drives GCT growth and a rationale for therapeutic targeting of GCTs with agents that antagonize the EGFR and mTORC1 pathways.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[St] Status:Publisher

  4 / 7889 MEDLINE  
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[PMID]: 29377304
[Au] Autor:Kusama K; Bai R; Imakawa K
[Ad] Address:Graduate School of Agricultural and Life Science, The University of Tokyo, Animal Resource Science Center, Kasama, Ibaraki, Japan.
[Ti] Title:Regulation of human trophoblast cell syncytialization by transcription factors STAT5B and NR4A3.
[So] Source:J Cell Biochem;, 2018 Jan 29.
[Is] ISSN:1097-4644
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:In human trophoblast cells, cyclic AMP or its inducer forskolin (FSK) activates two downstream signaling molecules, protein kinase A (PKA) and exchange protein directly activated by cAMP (EPAC), both of which induce syncytialization, cell fusion, and the production of human chorionic gonadotropin (hCG) and progesterone. However, a transcription factor other than GCM1 and molecular mechanisms associated with these events have not been well characterized. To identify novel transcription factors involved in syncytialization of cAMP-stimulated human choriocarcinoma BeWo cells, the microarray analysis was performed with RNAs extracted from PKA- or EPAC-selective cAMP analog-stimulated BeWo cells, from which two up-regulated transcription factors, STAT5 and NR4A3, were found. The knockdown of STAT5B decreased FSK-induced cell fusion and the expression of syncytialization markers, CGB, syncytin1, syncytin2, GCM1, and OVOL1, but NR4A3 knockdown increased FSK-induced cell fusion and the expression of CGB and syncytin2. These findings indicated that cAMP-PKA up-regulated STAT5B, followed by increase in syncytin2 expression through GCM1 and OVOL1, resulting in cell fusion and hCG production, while cAMP-PKA-up-regulated NR4A3 could decrease syncytin2 expression, and suggested that both positive and negative effects of STAT5B and NR4A3, respectively, are required to control the degree of syncytialization in human trophoblast cells.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[St] Status:Publisher
[do] DOI:10.1002/jcb.26721

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[PMID]: 29367697
[Au] Autor:Zhao JR; Cheng WW; Wang YX; Cai M; Wu WB; Zhang HJ
[Ad] Address:Departments of Pathology and Bio-Bank, International Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200030, China.
[Ti] Title:Identification of microRNA signature in the progression of gestational trophoblastic disease.
[So] Source:Cell Death Dis;9(2):94, 2018 Jan 24.
[Is] ISSN:2041-4889
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Gestational trophoblastic disease (GTD) encompasses a range of trophoblast-derived disorders. The most common type of GTD is hydatidiform mole (HM). Some of HMs can further develop into malignant gestational trophoblastic neoplasia (GTN). Aberrant expression of microRNA (miRNA) is widely reported to be involved in the initiation and progression of cancers. MiRNA expression profile also has been proved to be the useful signature for diagnosis, staging, prognosis, and response to chemotherapy. Till now, the profile of miRNA in the progression of GTD has not been determined. In this study, a total of 34 GTN and 60 complete HMs (CHM) trophoblastic tissues were collected. By miRNA array screening and qRT-PCR validating, six miRNAs, including miR-370-3p, -371a-5p, -518a-3p, -519d-3p, -520a-3p, and -934, were identified to be differentially expressed in GTN vs. CHM. Functional analyses further proved that miR-371a-5p and miR-518a-3p promoted proliferation, migration, and invasion of choriocarcinoma cells. Moreover, we demonstrated that miR-371a-5p was negatively related to protein levels of its predictive target genes BCCIP, SOX2, and BNIP3L, while miR-518a-3p was negatively related to MST1 and EFNA4. For the first time, we proved that miR-371a-5p and miR-518a-3p directly targeted to 3'-UTR regions of BCCIP and MST1, respectively. Additionally, we found that miR-371a-5p and miR-518a-3p regulated diverse pathways related to tumorigenesis and metastasis in choriocarcinoma cells. The results presented here may offer new clues to the progression of GTD and may provide diagnostic biomarkers for GTN.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1801
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[St] Status:In-Data-Review
[do] DOI:10.1038/s41419-017-0108-2

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[PMID]: 29223574
[Au] Autor:Kurosawa Y; Furugen A; Nishimura A; Narumi K; Kobayashi M; Iseki K
[Ad] Address:Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, Kita-12-jo, Nishi-6-chome, Kita-ku, Sapporo 060-0812, Japan.
[Ti] Title:Evaluation of the effects of antiepileptic drugs on folic acid uptake by human placental choriocarcinoma cells.
[So] Source:Toxicol In Vitro;48:104-110, 2018 Apr.
[Is] ISSN:1879-3177
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Folate status during pregnancy is important for fetal development and health. The placenta plays an important role in supplying the fetus with folate. Most women with epilepsy continue their medication during pregnancy. In the present study, we aimed to evaluate the effects of 16 antiepileptic drugs, clinically used for treatment of epilepsy, on folic acid uptake in two in vitro placental models, BeWo and JEG-3 cells. Short-term exposure to antiepileptic drugs had no effects on [ H]-folic acid uptake by BeWo cells. However, long-term exposure (24h) to valproic acid (VPA) increased [ H]-folic acid uptake by BeWo and JEG-3 cells. VPA treatment for 24h increased folate receptor-α (FRα) and proton-coupled folate transporter (PCFT) mRNA expression; however, it did not affect reduced folate carrier expression. These results suggested that the increase in folic acid uptake after exposure to VPA can be attributed to the induction of FRα and PCFT expression. Furthermore, the present study showed that exposure to clinical concentrations of oxcarbazepine and stiripentol reduced the viability of BeWo cells. Therefore, the findings of the present study may contribute to better understanding of the mechanisms of toxicity of antiepileptic drugs, and estimation of their potential risk to fetus.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180305
[Lr] Last revision date:180305
[St] Status:In-Process

  7 / 7889 MEDLINE  
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[PMID]: 29493364
[Au] Autor:Sezgin-Bayindir Z; Elcin AE; Parmaksiz M; Elcin YM; Yuksel N
[Ad] Address:a Department of Pharmaceutical Technology, Faculty of Pharmacy , Ankara University , 06100 Tandogan , Ankara , Turkey.
[Ti] Title:Investigations on Clonazepam Loaded Polymeric Micelle-like Nanoparticles for Safe Drug Administration During Pregnancy.
[So] Source:J Microencapsul;:1-43, 2018 Mar 01.
[Is] ISSN:1464-5246
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Medication during pregnancy is often a necessity for women to treat their acute or chronic diseases. The goal of this study is to evaluate the potential of micelle-like nanoparticles (MNP) for providing safe drug usage in pregnancy and protect both fetus and mother from medication side effects. Clonazepam loaded MNP were prepared from copolymers (polystyrene-poly(acrylic acid) (PS-PAA), poly(ethylene glycol)-b-poly(lactic acid) (PEG-PLA) and distearyl-sn-glycero-3-phosphoethanolamine-N-[methoxy-poly(ethylene glycol) (PEG-DSPE)) with varying monomer ratios and their drug loading efficiency, drug release ratio, particle size, surface charge and morphology were characterized. The cellular transport and cytotoxicity experiments were conducted on clonazepam and MNP formulations using placenta-choriocarcinoma-BeWo and brain-endothelial-bEnd3 cells. Clonazepam loaded PEG -PLA MNP reduced the drug transport through BeWo cells demonstrating that MNP may lower fetal drug exposure, thus reduce the drug side effects. However, lipofectamine modified MNP improved the transport of clonazepam and found to be promising for brain and in-utero specific drug treatment.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180301
[Lr] Last revision date:180301
[St] Status:Publisher
[do] DOI:10.1080/02652048.2018.1447615

  8 / 7889 MEDLINE  
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[PMID]: 29465578
[Au] Autor:Wu CJ; Hwa HL; Chang WC; Hsu HC; Wu MZ; Sheu BC
[Ad] Address:Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, Taipei.
[Ti] Title:Short tandem repeat analysis for confirmation of uterine non-gestational choriocarcinoma in a postmenopausal Taiwanese woman.
[So] Source:Medicine (Baltimore);97(8):e9899, 2018 Feb.
[Is] ISSN:1536-5964
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:RATIONALE: Rare uterine choriocarcinoma can be differentiated gestational from nongestational choriocarcinoma by using short tandem repeats (STRs). PATIENT CONCERNS: A 56-year-old Taiwanese woman underwent staging surgery because of suspicion of high-grade endometrial cancer. The pathology-confirmed uterine tumor with syncytiotrophoblasts and decidual change of the endometrium was harvested. DIAGNOSIS: Uterine nongestational choriocarcinoma. INTERVENTIONS: The tumor specimen, the patient's blood, and her husband's blood were drawn for STRs analysis using polymerase chain reaction amplification kit. The genotype of the tumor cells was solely maternal and made the diagnosis of uterine nongestational choriocarcinoma. OUTCOME: Adjuvant chemotherapy with etoposide, methotrexate, actinomycin D, cyclophosphamide, vincristine regimen achieved good response in the patient. The patient is now recurrence-free for 12 months. LESSONS: STRs aid precise classification of rare choriocarcinoma. We encourage using the method to analyze suspicious choriocarcinoma.
[Mh] MeSH terms primary: Choriocarcinoma, Non-gestational/genetics
Choriocarcinoma, Non-gestational/pathology
Microsatellite Repeats
Neoplasm Staging/methods
Uterine Neoplasms/genetics
Uterine Neoplasms/pathology
[Mh] MeSH terms secundary: Antineoplastic Combined Chemotherapy Protocols/therapeutic use
Chemotherapy, Adjuvant
Choriocarcinoma, Non-gestational/drug therapy
Choriocarcinoma, Non-gestational/surgery
Female
Humans
Middle Aged
Postmenopause
Uterine Neoplasms/drug therapy
Uterine Neoplasms/surgery
[Pt] Publication type:CASE REPORTS; JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180227
[Lr] Last revision date:180227
[Js] Journal subset:AIM; IM
[Da] Date of entry for processing:180222
[St] Status:MEDLINE
[do] DOI:10.1097/MD.0000000000009899

  9 / 7889 MEDLINE  
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[PMID]: 29465790
[Au] Autor:Zhang Y; Zhang Y
[Ad] Address:Department of Obstetrics and gynecology, The Second People's Hospital of Yunan Province.
[Ti] Title:Forkhead box C2 promotes the invasion ability of human trophoblast cells through Hedgehog (Hh) signaling pathway.
[So] Source:Cell Biol Int;, 2018 Feb 21.
[Is] ISSN:1095-8355
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Embryonic development depends on the normal invasion of trophoblast cells. Forkhead box C2 (FOXC2) is a member of Forkhead box family, which is involved in the tumor cells invasion. The aim of this study was to explore the roles of FOXC2 on the invasion of human trophoblast cells, and further study its molecular mechanism. The mRNA and protein levels of FOXC2 in human normal trophoblast and choriocarcinoma cell lines were analyzed by quantitative real-time PCR (qRT-PCR) and western blot assays, respectively. Methylthiazolyldiphenyl-tetrazo lium bromide (MTT) and transwell assays were separately performed to detect the adhesion and invasion of normal trophoblast cells treated with exogenous FOXC2 and FOXC2 siRNA. QRT-PCR and western blot assays were used to test levels of the epithelial-mesenchymal transition (EMT)-related and Hedgehog (Hh) signaling pathway-related factors, respectively. Herein, our results found that the expression levels of FOXC2 in normal trophoblast cells were lower than choriocarcinoma cells. FOXC2 over-expression remarkably strengthened the adhesion and invasion abilities of normal trophoblast cells. Moreover, over-expression of FOXC2 significantly promoted the expression of human leukocyte antigen-G (HLA-G), matrix metalloproteinase-2 (MMP-2), Vimentin, sonic hedgehog (Shh), Glioma-associated oncogene homolog 1 (Gli1), and Snail, and inhibited E-cadherin expression. However, it showed the opposite tendency in FOXC2 siRNA group. In addition, there was no significant change in the expression of MMP9 among different groups. Above results illustrated that FOXC2 could promote the invasion ability of normal trophoblast cells by EMT-mediated Hh pathway.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180221
[Lr] Last revision date:180221
[St] Status:Publisher
[do] DOI:10.1002/cbin.10953

  10 / 7889 MEDLINE  
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[PMID]: 29286555
[Au] Autor:Dicken BJ; Billmire DF; Krailo M; Xia C; Shaikh F; Cullen JW; Olson TA; Pashankar F; Malogolowkin MH; Amatruda JF; Rescorla FJ; Egler RA; Ross JH; Rodriguez-Galindo C; Frazier AL
[Ad] Address:Stollery Children's Hospital, University of Alberta Hospital, Edmonton, Alberta, Canada.
[Ti] Title:Gonadal dysgenesis is associated with worse outcomes in patients with ovarian nondysgerminomatous tumors: A report of the Children's Oncology Group AGCT 0132 study.
[So] Source:Pediatr Blood Cancer;65(4), 2018 Apr.
[Is] ISSN:1545-5017
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PURPOSE: In this report, we characterize the timing and behavior of malignant ovarian germ cell tumors (GCTs) in pediatric patients with dysgenetic gonads compared to those with normal gonadal development. PATIENTS AND METHODS: Patients from the Children's Oncology Group AGCT0132 with malignant ovarian GCTs were included. Within this population, we sought to identify patients with gonadoblastoma, streak ovaries, or other evidence of gonadal dysgenesis (GD). Patients with malignant GCTs containing one or more of the following histologies-yolk sac tumor, embryonal carcinoma, or choriocarcinoma-were included. Patients were compared with respect to event-free survival (EFS) and overall survival (OS). RESULTS: Nine patients with GD, including seven with gonadoblastoma (mean age, 9.3 years), were compared to 100 non-GD patients (mean age, 12.1 years). The estimated 3-year EFS for patients with GD was 66.7% (95% CI 28.2-87.8%) and for non-GD patients was 88.8% (95% CI 80.2-93.8%). The estimated 3-year OS for patients with GD was 87.5% (95% CI 38.7-98.1%) and for non-GD patients was 97.6% (95% CI of 90.6-99.4%). CONCLUSION: Patients presenting with nongerminomatous malignant ovarian GCTs in the context of GD have a higher rate of events and death than counterparts with normal gonads. These findings emphasize the importance of noting a contralateral streak ovary or gonadoblastoma at histology for any ovarian GCT and support the recommendation for early bilateral gonadectomy in patients known to have GD with Y chromosome material. In contrast to those with pure dysgerminoma, these patients may represent a high-risk group that requires a more aggressive chemotherapy regimen.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180221
[Lr] Last revision date:180221
[St] Status:In-Data-Review
[do] DOI:10.1002/pbc.26913


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