Database : MEDLINE
Search on : epithelium [Words]
References found : 270269 [refine]
Displaying: 1 .. 10   in format [Detailed]

page 1 of 27027 go to page                         

  1 / 270269 MEDLINE  
              next record last record
select
to print
Photocopy

[PMID]: 27796960
[Au] Autor:van de Moosdijk AA; Fu NY; Rios AC; Visvader JE; van Amerongen R
[Ad] Address:Section of Molecular Cytology and Van Leeuwenhoek Centre for Advanced Microscopy, Swammerdam Institute for Life Sciences, Universityof Amsterdam, Science Park 904, 1098 XH, Amsterdam, The Netherlands.
[Ti] Title:Lineage Tracing of Mammary Stem and Progenitor Cells.
[So] Source:Methods Mol Biol;1501:291-308, 2017.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:Lineage tracing analysis allows mammary epithelial cells to be tracked in their natural environment, thereby revealing cell fate and proliferation choices in the intact tissue. This technique is particularly informative for studying how stem cells build and maintain the mammary epithelium during development and pregnancy. Here we describe two experimental systems based on Cre/loxP technology (Cre(ERT2)/loxP and rtTA/tetO-Cre/loxP), which allow the inducible, permanent labeling of mammary epithelial cells following the administration of either tamoxifen or doxycycline.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  2 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 27796959
[Au] Autor:Smith GH; Boulanger CA
[Ad] Address:Mammary Stem Cell Section, Basic Research Laboratory, National Cancer Institute, National Institutes of Health, Building 37 Rm 1112, 37 Convent Drive, Bethesda, MD, 20892, USA. smithg@mail.nih.gov.
[Ti] Title:Techniques for the Reprogramming of Exogenous Stem/Progenitor Cell Populations Towards a Mammary Epithelial Cell Fate.
[So] Source:Methods Mol Biol;1501:277-289, 2017.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:This chapter considers the techniques necessary and required for the reprogramming of exogenous stem/progenitor cell populations towards a mammary epithelial cell fate. The protocols describe how to isolate cells from alternate mouse organs such as testicles of male mice and mix them with mammary cells to generate chimeric glands comprised of male and female epithelial cells that are fully competent. During the reformation of mammary stem cell niches by dispersed epithelial cells, in the context of the intact epithelium-free mammary stroma, non-mammary cells are sequestered and reprogrammed to perform mammary epithelial cell functions including those ascribed to mammary stem/progenitor cells. This therefore is a powerful technique for the redirection of cells from other organs/cancer cells to a normal mammary phenotype.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  3 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 27796958
[Au] Autor:Shehata M; Stingl J
[Ad] Address:Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Robinson Way, Cambridge, CB2 0RE, UK. mona.shehata@uhnresearch.ca.
[Ti] Title:Purification of Distinct Subsets of Epithelial Cells from Normal Human Breast Tissue.
[So] Source:Methods Mol Biol;1501:261-276, 2017.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:The mammary epithelium is composed of a variety of specialized cell types that function in a coordinated fashion to produce and eject milk through multiple cycles of pregnancy. The ability to identify and purify these subsets of cells in order to interrogate their growth and differentiation capacities, as well as to characterize the molecular mechanisms that regulate their behavior, is essential in identifying the processes associated with breast cancer initiation and progression. This methods chapter outlines the step-by-step methods for dissociating human breast reduction specimens to a single cell suspension of viable cells. As well, strategies for purifying four distinct subsets of epithelial cells by using fluorescence-activated cell sorting and protocols for interrogating the growth and differentiation properties of these purified cells at clonal densities in adherent culture are also described.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  4 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 27796955
[Au] Autor:Koledova Z; Lu P
[Ad] Address:Wellcome Trust Centre for Cell Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, M13 9PT, UK. zkoledova@gmail.com.
[Ti] Title:A 3D Fibroblast-Epithelium Co-culture Model for Understanding Microenvironmental Role in Branching Morphogenesis of the Mammary Gland.
[So] Source:Methods Mol Biol;1501:217-231, 2017.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:The mammary gland consists of numerous tissue compartments, including mammary epithelium, an array of stromal cells, and the extracellular matrix (ECM). Bidirectional interactions between the epithelium and its surrounding stroma are essential for proper mammary gland development and homeostasis, whereas their deregulation leads to developmental abnormalities and cancer. To study the relationships between the epithelium and the stroma, development of models that could recapitulate essential aspects of these interacting systems in vitro has become necessary. Here we describe a three-dimensional (3D) co-culture assay and show that the addition of fibroblasts to mammary organoid cultures promotes the epithelium to undergo branching morphogenesis, thus allowing the role of the stromal microenvironment to be examined in this essential developmental process.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  5 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 27796951
[Au] Autor:Ibrahim AM; Cairney C; Morris JS; Stein T
[Ad] Address:Institute of Cancer Sciences, College of MVLS, University of Glasgow, Glasgow, G12 8QQ, UK.
[Ti] Title:RNA Profiling of Non-cultured Fibroblasts Isolated from Pubertal Mouse Mammary Gland Sections.
[So] Source:Methods Mol Biol;1501:149-164, 2017.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:The epithelium of the pubertal mouse mammary gland grows and invades the mammary fat pad to form a primary ductal network. This outgrowth is tightly controlled by epithelial and stromal factors that are present in the environment around the terminal end buds (TEB) at the growth front and the newly formed ducts. Identifying the contribution that each cell type makes to this regulation is a major challenge. To identify the role that fibroblasts play during this process we have optimised a fibroblast isolation procedure, followed by cell cleanup, RNA extraction, and amplification from non-cultured, freshly isolated fibroblasts from around the TEB as well as the subtending ducts. This was facilitated by the use of mice that constitutively expressed EGFP, which allowed the visualization of the growth front of the pubertal mammary tree under UV light. The isolated RNA is of sufficiently high quality, giving reproducible qRT-PCR results, for transcriptome analysis after RNA amplification.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  6 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 27796950
[Au] Autor:Morris JS; Stein T
[Ad] Address:School of Veterinary Medicine, College of MVLS, University of Glasgow, Glasgow, UK.
[Ti] Title:Pubertal Ductal Morphogenesis: Isolation and Transcriptome Analysis of the Terminal End Bud.
[So] Source:Methods Mol Biol;1501:131-148, 2017.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:The terminal end bud (TEB) is the growing part of the ductal mammary epithelium during puberty, enabling the formation of a primary epithelial network. These highly proliferative bulbous end structures that drive the ductal expansion into the mammary fat pad comprise an outer cap cell layer, containing the progenitor cells of the ductal myoepithelium, and the body cells, which form the luminal epithelium. As TEB make up only a very small part of the whole mammary tissue, TEB-associated factors can be easily missed when whole-tissue sections are being analyzed. Here we describe a method to enzymatically separate TEB and ducts, respectively, from the surrounding stroma of pubertal mice in order to perform transcriptomic or proteomic analysis on the isolated structures and identify potential novel regulators of epithelial outgrowth, or to allow further cell culturing. This approach has previously allowed us to identify novel TEB-associated proteins, including several axonal guidance proteins. We further include protocols for the culturing of isolated TEB, processing of mammary tissue into paraffin and immunohistochemical/fluorescent staining for verification, and localization of protein expression in the mammary tissue at different developmental time points.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  7 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 27796949
[Au] Autor:Buric D; Brisken C
[Ad] Address:The Swiss Institute for Experimental Cancer Research (ISREC), Swiss Federal Institute of Technology (EPFL), Station 19, CH-1015, Lausanne, Switzerland.
[Ti] Title:Analysis of Mammary Gland Phenotypes by Transplantation of the Genetically Marked Mammary Epithelium.
[So] Source:Methods Mol Biol;1501:115-129, 2017.
[Is] ISSN:1940-6029
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:The mammary gland is the only organ to undergo most of its development after birth and therefore particularly attractive for studying developmental processes. In the mouse, powerful tissue recombination techniques are available that can be elegantly combined with the use of different genetically engineered mouse models to study development and differentiation in vivo.In this chapter, we describe how epithelial intrinsic gene function can by discerned by grafting mammary epithelial cells of different genotypes to wild-type recipients. Either pieces of mammary epithelial tissue or dissociated mammary epithelial cells are isolated from donor mice and subsequently transplanted into recipients whose mammary fat pads were divested of their endogenous epithelium. This is followed by phenotypic characterization of the epithelial outgrowth either by fluorescence stereomicroscopy for the fluorescently marked grafts or carmine alum whole mount for the unmarked epithelia.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  8 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 27796942
[Au] Autor:Langwinski W; Narozna B; Lackie PM; Holloway JW; Szczepankiewicz A
[Ad] Address:Laboratory of Molecular and Cell Biology, Department of Pediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 27/33 Szpitalna St., 60-572, Poznan, Poland.
[Ti] Title:Comparison of miRNA profiling during airway epithelial repair in undifferentiated and differentiated cells in vitro.
[So] Source:J Appl Genet;, 2016 Oct 28.
[Is] ISSN:2190-3883
[Cp] Country of publication:England
[La] Language:ENG
[Ab] Abstract:Respiratory epithelium is a highly integrated structure that efficiently protects lungs from extrinsic irritants thanks to rapid repair of the wound. The repair is a complex process that requires coordinated expression of networks of genes. Plausible regulators of this process are microRNAs. We investigated whether global miRNA silencing influences the epithelial repair, and whether changes in miRNA expression profile during repair are similar between two bronchial epithelial cell cultures: differentiated and undifferentiated cells. Two bronchial cell types were used:16HBE14o- and NHBE. Transfection was performed with siRNAs against Drosha and Dicer. For miRNA profiling, non-transfected cells were cultured until confluent and harvested for RNA isolation at baseline (cells before wounding) and at different time post-wounding (8, 16, 24, and 48 h). MicroRNA expression profiling was performed using TaqMan Array Human MicroRNA Card A. Target prediction was done in miRNA body map, and pathway analysis using DAVID. Cells with downregulated Drosha and Dicer demonstrated a significantly delayed wound repair in comparison to control in both cell lines. MiRNA expression profiling revealed that ten miRNAs exhibited significant changes over time after cell injury. These genes showed a similar expression pattern in both cell lines. The predicted targets of these miRNAs were then clustered by pathway analysis into six biological groups related to wound repair. Silencing of global miRNA expression confirmed that miRNAs are crucial for airway epithelial repair. Moreover, epithelial cells of two different origins demonstrated some similarities in miRNA expression pattern during wound repair, independent of differentiation state.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:Publisher

  9 / 270269 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Taboga, Sebastiäo R

[PMID]: 27796889
[Au] Autor:Sanches BD; Corradi LS; Vilamaior PS; Taboga SR
[Ad] Address:Department of Structural and Functional Biology, State University of Campinas - UNICAMP, Bertrand Russel Av. s/n, Campinas, São Paulo, Brazil.
[Ti] Title:Paracrine Signaling in the Prostatic Stroma: A Novel Role for the Telocytes Revealed in Rodents' Ventral Prostate.
[So] Source:Adv Exp Med Biol;913:193-206, 2016.
[Is] ISSN:0065-2598
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:The telocytes have recently been described in the prostate gland. In mature gland, they exist in close association with the acini and their telopodes form networks whose functions remain unclear. In this chapter, our group gives a brief introduction to telocytes and explores the history that led to such a concept and then discusses hypotheses and presents new evidences about the roles exerted by telocytes in the prostate. First is given emphasis on the role that these cells possibly play in paracrine signaling employed in the differentiation of smooth muscle periacinar are then discussed other roles potentially performed by telocytes in the prostate, such as the organizational, where these cells would act in order to delimit stromal microenvironments, thereby assisting the differentiation of the prostatic anatomical components. In addition, the pacemaker function of smooth muscle cells contraction, as evidenced by the presence of caveolae and gap-type junction and, finally, the role of telocytes in prostate remodeling and the possible action as adult progenitor cells. Generally speaking, the chapter reaffirms the existence of telocytes as distinct cells of other stromal cells and the importance of this new cell type for normal metabolism and prostate development.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:In-Data-Review

  10 / 270269 MEDLINE  
              first record previous record
select
to print
Photocopy

[PMID]: 27796462
[Au] Autor:Wang JY; Liu Y; Song LJ; Lv F; Xu XJ; San A; Wang J; Yang HM; Yang ZY; Jiang Y; Wang O; Xia WB; Xing XP; Li M
[Ad] Address:Department of Endocrinology, Key Laboratory of Endocrinology of Ministry of Health, Peking Union Medical College Hospital, Chinese Academy of Medical Science, Shuaifuyuan No. 1, Dongcheng District, Beijing, 100730, China.
[Ti] Title:Novel Mutations in SERPINF1 Result in Rare Osteogenesis Imperfecta Type VI.
[So] Source:Calcif Tissue Int;, 2016 Oct 28.
[Is] ISSN:1432-0827
[Cp] Country of publication:United States
[La] Language:ENG
[Ab] Abstract:Osteogenesis imperfecta (OI) is a group of inherited disorders characterized by recurrent fragile fractures. Serpin peptidase inhibitor, clade F, member 1 (SERPINF1) is known to cause a distinct, extremely rare autosomal recessive form of type VI OI. Here we report, for the first time, the detection of SERPINF1 mutations in Chinese OI patients. We designed a novel targeted next-generation sequencing panel of OI-related genes to identify pathogenic mutations, which were confirmed with Sanger sequencing and by co-segregation analysis. We also investigated the phenotypes of OI patients by evaluating bone mineral density, radiological fractures, serum bone turnover markers, and pigment epithelium-derived factor (PEDF) concentration. Six patients with moderate-to-severe bone fragility, significantly low bone mineral density, and severe deformities of the extremities were recruited from five unrelated families for this study. Six pathogenic mutations in SERPINF1 gene were identified, five of which were novel: (1) a homozygous in-frame insertion in exon 3 (c.271_279dup, p.Ala91_Ser93dup); (2) compound heterozygous mutations in intron 3 (c.283 + 1G > T, splicing site) and exon 5 (c.498_499delCA, p.Arg167SerfsX35, frameshift); (3) a homozygous frameshift mutation in exon 8 (c.1202_1203delCA, p.Thr401ArgfsX); (4) compound heterozygous missense mutation (c.184G > A, p.Gly62Ser) and in-frame insertion (c.271_279dup, p.Ala91_Ser93dup) in exon 3; and (5) a heterozygous nonsense mutation in exon 4 (c.397C>T + ?, p.Gln133X + ?). Serum PEDF levels were barely detectable in almost all subjects. We identified five novel mutations in SERPINF1 and confirmed the diagnostic value of serum PEDF level for the first time in Chinese patients with the extremely rare OI type VI.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1610
[Cu] Class update date: 161031
[Lr] Last revision date:161031
[St] Status:Publisher


page 1 of 27027 go to page                         
   


Refine the search
  Database : MEDLINE Advanced form   

    Search in field  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/PAHO/WHO - Latin American and Caribbean Center on Health Sciences Information