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[PMID]: 25834395
[Au] Autor:Winnicka K; Wroblewska M; Sosnowska K; Car H; Kasacka I
[Ad] Address:Department of Pharmaceutical Technology, Faculty of Pharmacy, Medical University of Bialystok, Bialystok, Poland....
[Ti] Title:Evaluation of cationic polyamidoamine dendrimers' dermal toxicity in the rat skin model.
[So] Source:Drug Des Devel Ther;9:1367-77, 2015.
[Is] ISSN:1177-8881
[Cp] Country of publication:New Zealand
[La] Language:eng
[Ab] Abstract:Polyamidoamine (PAMAM) dendrimers are multi-branched, three-dimensional polymers with unique architecture, which makes these molecules attractive for medical and pharmaceutical applications. Using PAMAM as drug carriers for topical delivery might be beneficial as they only produce a transient effect without skin irritation. To evaluate the dermal toxicity of cationic PAMAM dendrimers generation 2 and generation 3, skin irritation studies were performed in vivo in the rat skin model. After 10 days topical application of various concentrations of PAMAM-NH2 (0.3 mg/mL, 3 mg/mL, 6 mg/mL, 30 mg/mL, 300 mg/mL), skin irritation was evaluated by visual, histopathological, and immunohistochemical examination. Microscopic assessment after hematoxylin-eosin staining revealed significant morphological changes of epidermal cells after application of PAMAM-NH2 at a concentration of ≥6 mg/mL. Morphological alterations of epidermal cells included cytoplasmic vacuolization of keratinocytes in the basal and spinous layers. Cytomorphological changes in keratinocytes, overall picture of the epidermis, and histopathological changes in the dermis were dose dependent. Detected alterations concerned hyperplasia of connective tissue fibers and leukocyte infiltration. Visible granulocyte infiltration in the upper dermis and sockets formed by necrotic, cornified cells in the hyperplastic foci of epithelium were also noted. Immunohistochemical analyses revealed that increased nuclear immunoreactivity to PCNA correlated with the concentration of PAMAM-NH2, but no significant differences in the cell proliferation activity in skin treated with PAMAM-NH2 generation 2 or generation 3 were observed. Significantly higher expression of PCNA extended throughout the skin layers might suggest abnormal cell proliferation, which, as a consequence, might even lead to neoplastic changes.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1504
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.2147/DDDT.S78336

  2 / 255062 MEDLINE  
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[PMID]: 25834386
[Au] Autor:Rossi S; Orrico A; Santamaria C; Romano V; De Rosa L; Simonelli F; De Rosa G
[Ad] Address:Multidisciplinary Department of Medical, Surgical and Dental Sciences, Eye Clinic, Second University of Naples, Naples, Italy....
[Ti] Title:Standard versus trans-epithelial collagen cross-linking in keratoconus patients suitable for standard collagen cross-linking.
[So] Source:Clin Ophthalmol;9:503-9, 2015.
[Is] ISSN:1177-5467
[Cp] Country of publication:New Zealand
[La] Language:eng
[Ab] Abstract:PURPOSE: Evaluating the clinical results of trans-epithelial collagen cross-linking (CXL) and standard CXL in patients with progressive keratoconus. METHODS: This prospective study comprised 20 eyes of 20 patients with progressive keratoconus. Ten eyes were treated by standard CXL and ten by trans-epithelial cross-linking (TE-CXL, epithelium on) with 1 year of follow-up. All patients underwent complete ophthalmologic testing that included pre- and postoperative uncorrected visual acuity, corrected visual acuity, spherical error, spherical equivalent, corneal astigmatism, simulated maximum, minimum, and average keratometry, coma and spherical aberration, optical pachymetry, and endothelial cell density. Intra-and postoperative complications were recorded. The solution used for standard CXL comprised riboflavin 0.1% and dextran 20.0% (Ricrolin), while the solution for TE-CXL (Ricrolin, TE) comprised riboflavin 0.1%, dextran 15.0%, trometamol (Tris), and ethylenediaminetetraacetic acid. Ultraviolet-A treatment was performed with UV-X System at 3 mW/cm(2). RESULTS: In both the standard CXL group (ten patients, ten eyes; mean age, 30.4±7.3 years) and the TE-CXL group (ten patients, ten eyes; mean age, 28±3.8 years), uncorrected visual acuity and corrected visual acuity improved significantly after treatment. Furthermore, a significant improvement in topographic outcomes, spherical error, and spherical equivalent was observed in both groups at month 12 posttreatment. No significant variations were recorded in other parameters. No complications were noted. CONCLUSION: A 1-year follow-up showed stability of clinical and refractive outcomes after standard CXL and TE-CXL.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1504
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Da] Date of entry for processing:150402
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.2147/OPTH.S73991

  3 / 255062 MEDLINE  
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[PMID]: 25188360
[Au] Autor:Morales-Nebreda LI; Rogel MR; Eisenberg JL; Hamill KJ; Soberanes S; Nigdelioglu R; Chi M; Cho T; Radigan KA; Ridge KM; Misharin AV; Woychek A; Hopkinson S; Perlman H; Mutlu GM; Pardo A; Selman M; Jones JC; Budinger GR
[Ad] Address:1 Division of Pulmonary and Critical Care Medicine and the Department of Cell and Molecular Biology, Feinberg School of Medicine at Northwestern University, Chicago, Illinois.
[Ti] Title:Lung-Specific Loss of α3 Laminin Worsens Bleomycin-Induced Pulmonary Fibrosis.
[So] Source:Am J Respir Cell Mol Biol;52(4):503-12, 2015 Apr.
[Is] ISSN:1535-4989
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Laminins are heterotrimeric proteins that are secreted by the alveolar epithelium into the basement membrane, and their expression is altered in extracellular matrices from patients with pulmonary fibrosis. In a small number of patients with pulmonary fibrosis, we found that the normal basement membrane distribution of the α3 laminin subunit was lost in fibrotic regions of the lung. To determine if these changes play a causal role in the development of fibrosis, we generated mice lacking the α3 laminin subunit specifically in the lung epithelium by crossing mice expressing Cre recombinase driven by the surfactant protein C promoter (SPC-Cre) with mice expressing floxed alleles encoding the α3 laminin gene (Lama3(fl/fl)). These mice exhibited no developmental abnormalities in the lungs up to 6 months of age, but, compared with control mice, had worsened mortality, increased inflammation, and increased fibrosis after the intratracheal administration of bleomycin. Similarly, the severity of fibrosis induced by an adenovirus encoding an active form of transforming growth factor-ß was worse in mice deficient in α3 laminin in the lung. Taken together, our results suggest that the loss of α3 laminin in the lung epithelium does not affect lung development, but plays a causal role in the development of fibrosis in response to bleomycin or adenovirally delivered transforming growth factor-ß. Thus, we speculate that the loss of the normal basement membrane organization of α3 laminin that we observe in fibrotic regions from the lungs of patients with pulmonary fibrosis contributes to their disease progression.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1504
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1165/rcmb.2014-0057OC

  4 / 255062 MEDLINE  
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[PMID]: 25827591
[Au] Autor:Senthilkumaran C; Hewson J; Ollivett TL; Bienzle D; Lillie BN; Clark M; Caswell JL
[Ti] Title:Localization of annexins A1 and A2 in the respiratory tract of healthy calves and those experimentally infected with Mannheimia haemolytica.
[So] Source:Vet Res;46(1):6, 2015.
[Is] ISSN:1297-9716
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:Annexins A1 and A2 are proteins known to function in the stress response, dampening inflammatory responses and mediating fibrinolysis. We found, in healthy cattle recently arrived to a feedlot, that lower levels of these proteins correlated with later development of pneumonia. Here we determine the localization of annexin A1 and A2 proteins in the respiratory tract and in leukocytes, in healthy calves and those with Mannheimia haemolytica pneumonia. In healthy calves, immunohistochemistry revealed cytoplasmic expression of annexin A1 in the surface epithelium of large airways, tracheobronchial glands and goblet cells, to a lesser degree in small airways, but not in alveolar epithelium. Immunocytochemistry labeled annexin A1 in the cytoplasm of neutrophils from blood and bronchoalveolar lavage fluid, while minimal surface expression was detected by flow cytometry in monocytes, macrophages and lymphocytes. Annexin A2 expression was detected in surface epithelium of small airways, some mucosal lymphocytes, and endothelium, with weak expression in large airways, tracheobronchial glands and alveolar septa. For both proteins, the level of expression was similar in tissues collected five days after intrabronchial challenge with M. haemolytica compared to that from sham-inoculated calves. Annexins A1 and A2 were both detected in leukocytes around foci of coagulative necrosis, and in necrotic cells in the center of these foci, as well as in areas outlined above. Thus, annexins A1 and A2 are proteins produced by airway epithelial cells that may prevent inflammation in the healthy lung and be relevant to development of pneumonia in stressed cattle.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1504
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1186/s13567-014-0134-3

  5 / 255062 MEDLINE  
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[PMID]: 25829889
[Au] Autor:Bazerbachi F; Kermanshahi TR; Monteiro C
[Ad] Address:Department of Medicine, University of Minnesota, Minneapolis, MN.
[Ti] Title:Early precursor of mixed endocrine-exocrine tumors of the gastrointestinal tract: histologic and molecular correlations.
[So] Source:Ochsner J;15(1):97-101, 2015.
[Is] ISSN:1524-5012
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Mixed endocrine-exocrine tumors display histologic features of endocrine and glandular differentiation. Unlike in collision tumors, the two components are thought to arise from a monoclonal precursor. Evidence from molecular testing supports the monoclonal theory and suggests that the exocrine component may give rise to the endocrine component but not vice versa. CASE REPORT: We report a case of an adenomatous polyp in the large intestine that had groups of endocrine cells arising from the crypt bases of the adenomatous (exocrine) epithelium. To our knowledge, ours is only the second report of an adenomatous polyp in which groups of microcarcinoid endocrine cells were recognized. The histologic findings in our case correlate with the molecular findings described in mixed endocrine-exocrine tumors. CONCLUSION: Our description may represent the primordial stage of a mixed endocrine-exocrine neoplasm.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1504
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Da] Date of entry for processing:150401
[St] Status:PubMed-not-MEDLINE

  6 / 255062 MEDLINE  
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[PMID]: 25816372
[Au] Autor:Huang YH; I CC; Kuo CH; Hsu YY; Lee FT; Shi GY; Tseng SH; Wu HL
[Ad] Address:Institute of Clinical Medicine, National Cheng Kung University Medical College, Tainan, Taiwan; Department of Ophthalmology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan....
[Ti] Title:Thrombomodulin promotes corneal epithelial wound healing.
[So] Source:PLoS One;10(3):e0122491, 2015.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:PURPOSE: To determine the role of thrombomodulin (TM) in corneal epithelial wound healing, and to investigate whether recombinant TM epidermal growth factor-like domain plus serine/threonine-rich domain (rTMD23) has therapeutic potential in corneal epithelial wound healing. METHODS: TM localization and expression in the murine cornea were examined by immunofluorescence staining. TM expression after injury was also studied. The effect of rTMD23 on corneal wound healing was evaluated by in vitro and in vivo assays. RESULTS: TM was expressed in the cornea in normal adult mice. TM expression increased in the early phase of wound healing and decreased after wound recovery. In the in vitro study, platelet-derived growth factor-BB (PDGF-BB) induced TM expression in murine corneal epithelial cells by mediating E26 transformation-specific sequence-1 (Ets-1) via the mammalian target of rapamycin (mTOR) signaling pathway. The administration of rTMD23 increased the rate of corneal epithelial wound healing. CONCLUSIONS: TM expression in corneal epithelium was modulated during the corneal wound healing process, and may be regulated by PDGF-BB. In addition, rTMD23 has therapeutic potential in corneal injury.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1503
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1371/journal.pone.0122491

  7 / 255062 MEDLINE  
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[PMID]: 25810800
[Au] Autor:Laghmari M; Lezrek O
[Ad] Address:University Mohammed V Souissi, Faculty of Medicine and Pharmacy, Rabat, Moroco.
[Ti] Title:Congenital hypertrophy of the retinal pigment epithelium in Gardner's syndrome.
[So] Source:Pan Afr Med J;19:164, 2014.
[Is] ISSN:1937-8688
[Cp] Country of publication:Uganda
[La] Language:eng
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1503
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.11604/pamj.2014.19.164.4518

  8 / 255062 MEDLINE  
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[PMID]: 25807252
[Au] Autor:Luo J; Chen X; Pan YW; Lu S; Xia Z; Storm DR
[Ad] Address:Department of Pharmacology, University of Washington, Seattle, Washington, United States of America; College of Life Sciences, Wuhan University, Wuhan, Hubei, China....
[Ti] Title:The type 3 adenylyl cyclase is required for the survival and maturation of newly generated granule cells in the olfactory bulb.
[So] Source:PLoS One;10(3):e0122057, 2015.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The type 3 adenylyl cyclase (AC3) is localized to olfactory cilia in the main olfactory epithelium (MOE) and primary cilia in the adult mouse brain. Although AC3 has been strongly implicated in odor perception and olfactory sensory neuron (OSN) targeting, its role in granule cells (GCs), the most abundant interneurons in the main olfactory bulb (MOB), remains largely unknown. Here, we report that the deletion of AC3 leads to a significant reduction in the size of the MOB as well as the level of adult neurogenesis. The cell proliferation and cell cycle in the subventricular zone (SVZ), however, are not suppressed in AC3-/- mice. Furthermore, AC3 deletion elevates the apoptosis of GCs and disrupts the maturation of newly formed GCs. Collectively, our results identify a fundamental role for AC3 in the development of adult-born GCs in the MOB.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1503
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:IM
[St] Status:In-Data-Review
[do] DOI:10.1371/journal.pone.0122057

  9 / 255062 MEDLINE  
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[PMID]: 25794708
[Au] Autor:Wang Y; Shi C; Lu Y; Poulin EJ; Franklin JL; Coffey RJ
[Ad] Address:Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee....
[Ti] Title:Loss of lrig1 leads to expansion of brunner glands followed by duodenal adenomas with gastric metaplasia.
[So] Source:Am J Pathol;185(4):1123-34, 2015 Apr.
[Is] ISSN:1525-2191
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Leucine-rich repeats and immunoglobulin-like domains 1 (LRIG1) is a pan-ErbB negative regulator and intestinal stem cell marker down-regulated in many malignancies. We previously reported that 14 of 16 Lrig1-CreERT2/CreERT2 (Lrig1(-/-)) mice developed duodenal adenomas, providing the first in vivo evidence that Lrig1 acts as a tumor suppressor. We extended this study to a larger cohort and found that 49 of 54 Lrig1(-/-) mice develop duodenal adenomas beginning at 3 months. Most adenomas were histologically low grade and overlaid expanded Brunner glands. There was morphologic and biochemical blurring of the boundary between the epithelium and Brunner glands with glandular coexpression of ErbB2, which is normally restricted to the epithelium, and the Brunner gland marker Mucin6. Some adenomas were high grade with reduced Brunner glands. At age 4 to 5 weeks, before adenoma formation, we observed enhanced proliferation in Brunner glands and, at 2 months, an increase in the size of the Brunner gland compartment. Elevated expression of the epidermal growth factor receptor (Egfr) ligands amphiregulin and ß-cellulin, as well as Egfr and phosphorylated Egfr, was detected in adenomas compared with adjacent normal tissue. These adenomas expressed the gastric-specific genes gastrokine1 and mucin5ac, indicating gastric metaplasia. Moreover, we found that a subset of human duodenal tumors exhibited features of LRIG1(-/-) adenomas, including loss of LRIG1, gastric metaplasia (MUCIN5AC and MUCIN6), and increased amphiregulin and Egfr activity.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1503
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:AIM; IM
[St] Status:In-Data-Review

  10 / 255062 MEDLINE  
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[PMID]: 25794707
[Au] Autor:Xiao W; Dong G; Pacios S; Alnammary M; Barger LA; Wang Y; Wu Y; Graves DT
[Ad] Address:Department of Periodontology, School and Hospital of Stomatology, Peking University, Beijing, China; Department of Periodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pennsylvania....
[Ti] Title:FOXO1 Deletion Reduces Dendritic Cell Function and Enhances Susceptibility to Periodontitis.
[So] Source:Am J Pathol;185(4):1085-93, 2015 Apr.
[Is] ISSN:1525-2191
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The host response plays both protective and destructive roles in periodontitis. FOXO1 is a transcription factor that is activated in dendritic cells (DCs), but its function in vivo has not been examined. We investigated the role of FOXO1 in activating DCs in experimental (CD11c.Cre(+).FOXO1(L/L)) compared with matched control mice (CD11c.Cre(-).FOXO1(L/L)) in response to oral pathogens. Lineage-specific FOXO1 deletion reduced the recruitment of DCs to oral mucosal epithelium by approximately 40%. FOXO1 was needed for expression of genes that regulate migration, including integrins αν and ß3 and matrix metalloproteinase-2. Ablation of FOXO1 in DCs significantly decreased IL-12 produced by DCs in mucosal surfaces. Moreover, FOXO1 deletion reduced migration of DCs to lymph nodes, reduced capacity of DCs to induce formation of plasma cells, and reduced production of bacteria-specific antibody. The decrease in DC function in the experimental mice led to increased susceptibility to periodontitis through a mechanism that involved a compensatory increase in osteoclastogenic factors, IL-1ß, IL-17, and RANKL. Thus, we reveal a critical role for FOXO1 in DC recruitment to oral mucosal epithelium and activation of adaptive immunity induced by oral inoculation of bacteria.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1503
[Cu] Class update date: 150404
[Lr] Last revision date:150404
[Js] Journal subset:AIM; IM
[St] Status:In-Data-Review


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