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[PMID]: 29054449
[Au] Autor:Jiang Z; Li Z; Yue N; Zhang K; Li D; Zhang Y
[Ad] Address:State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, P.R. China.
[Ti] Title:Construction of infectious clones of lychnis ringspot virus and evaluation of its relationship with barley stripe mosaic virus by reassortment of genomic RNA segments.
[So] Source:Virus Res;243:106-109, 2018 01 02.
[Is] ISSN:1872-7492
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Lychnis ringspot virus (LRSV, genus Hordeivirus) was first isolated in 1959, and has been shown to infect several dicot plants in nature. However, due to the lack of infectious cDNA clones, the biological properties and mechanisms underlying LRSV infection are obscure. In this work, we constructed infectious cDNA clones of LRSV and have compiled the complete LRSV genomic (g) RNA sequence. Comparison of nucleotide and amino acid sequences between LRSV and barley stripe mosaic virus (BSMV), the type member of genus Hordeivirus, reveals that despite belonging to the same genus, and replicating in chloroplasts, the viruses are only distantly related. This could be further indicated by the failure of different LRSV/BSMV reassortants to infect N. benthamiana. LRSV infectious cDNA clones provide a useful tool for studies of biological diversity among hordeiviruses, and also may contribute to the understanding of seed transmission in dicot plants.
[Mh] MeSH terms primary: Lychnis/virology
Plant Diseases/virology
Plant Viruses/genetics
RNA Viruses/genetics
RNA, Viral/genetics
Recombination, Genetic
[Mh] MeSH terms secundary: Genome, Viral
Hordeum/virology
Plant Viruses/classification
Plant Viruses/isolation & purification
Plant Viruses/physiology
RNA Viruses/classification
RNA Viruses/isolation & purification
RNA Viruses/physiology
RNA, Viral/metabolism
Reassortant Viruses/classification
Reassortant Viruses/genetics
Reassortant Viruses/isolation & purification
Reassortant Viruses/physiology
Viral Proteins/genetics
Viral Proteins/metabolism
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (RNA, Viral); 0 (Viral Proteins)
[Em] Entry month:1801
[Cu] Class update date: 180209
[Lr] Last revision date:180209
[Js] Journal subset:IM
[Da] Date of entry for processing:171022
[St] Status:MEDLINE

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[PMID]: 28726198
[Au] Autor:Nesterova YV; Povet'eva TN; Zibareva LN; Suslov NI; Zueva EP; Aksinenko SG; Afanas'eva OG; Krylova SG; Amosova EN; Rybalkina OY; Lopatina KA
[Ad] Address:E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Tomsk, Russia. nes-yuliya@yandex.ru.
[Ti] Title:Anti-Inflammatory and Analgesic Activities of the Complex of Flavonoids from Lychnis chalcedonica L.
[So] Source:Bull Exp Biol Med;163(2):222-225, 2017 Jun.
[Is] ISSN:1573-8221
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Anti-inflammatory and analgesic activities of the complex of flavonoids from Lychnis chalcedonica L. were studied in the models of acute aseptic inflammation induced by carrageenan, histamine, and serotonin and acetic acid-induced painful chemical stimulation. It is demonstrated that course treatment with flavonoids derived from Lychnis chalcedonica L. produced a stable pharmacological effect comparable with that of the reference anti-inflammatory drug diclofenac.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1707
[Cu] Class update date: 170727
[Lr] Last revision date:170727
[St] Status:In-Process
[do] DOI:10.1007/s10517-017-3771-5

  3 / 55 MEDLINE  
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[PMID]: 28241056
[Au] Autor:Kang JS; Lee BY; Kwak M
[Ad] Address:Plant Resources Division, National Institute of Biological Resources, Incheon, Republic of Korea.
[Ti] Title:The complete chloroplast genome sequences of Lychnis wilfordii and Silene capitata and comparative analyses with other Caryophyllaceae genomes.
[So] Source:PLoS One;12(2):e0172924, 2017.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The complete chloroplast genomes of Lychnis wilfordii and Silene capitata were determined and compared with ten previously reported Caryophyllaceae chloroplast genomes. The chloroplast genome sequences of L. wilfordii and S. capitata contain 152,320 bp and 150,224 bp, respectively. The gene contents and orders among 12 Caryophyllaceae species are consistent, but several microstructural changes have occurred. Expansion of the inverted repeat (IR) regions at the large single copy (LSC)/IRb and small single copy (SSC)/IR boundaries led to partial or entire gene duplications. Additionally, rearrangements of the LSC region were caused by gene inversions and/or transpositions. The 18 kb inversions, which occurred three times in different lineages of tribe Sileneae, were thought to be facilitated by the intermolecular duplicated sequences. Sequence analyses of the L. wilfordii and S. capitata genomes revealed 39 and 43 repeats, respectively, including forward, palindromic, and reverse repeats. In addition, a total of 67 and 56 simple sequence repeats were discovered in the L. wilfordii and S. capitata chloroplast genomes, respectively. Finally, we constructed phylogenetic trees of the 12 Caryophyllaceae species and two Amaranthaceae species based on 73 protein-coding genes using both maximum parsimony and likelihood methods.
[Mh] MeSH terms primary: Caryophyllaceae/genetics
DNA, Chloroplast/genetics
Genome, Chloroplast
Lychnis/genetics
Silene/genetics
[Mh] MeSH terms secundary: DNA, Plant
Evolution, Molecular
Gene Duplication
Gene Rearrangement
Genome, Plant
Likelihood Functions
Microsatellite Repeats/genetics
Phylogeny
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (DNA, Chloroplast); 0 (DNA, Plant)
[Em] Entry month:1709
[Cu] Class update date: 170907
[Lr] Last revision date:170907
[Js] Journal subset:IM
[Da] Date of entry for processing:170228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0172924

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[PMID]: 27037925
[Au] Autor:Gizaw A; Brochmann C; Nemomissa S; Wondimu T; Masao CA; Tusiime FM; Abdi AA; Oxelman B; Popp M; Dimitrov D
[Ad] Address:Natural History Museum, University of Oslo, PO Box 1172 Blindern, NO-0318, Oslo, Norway.
[Ti] Title:Colonization and diversification in the African 'sky islands': insights from fossil-calibrated molecular dating of Lychnis (Caryophyllaceae).
[So] Source:New Phytol;211(2):719-34, 2016 Jul.
[Is] ISSN:1469-8137
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:The flora on the isolated high African mountains or 'sky islands' is remarkable for its peculiar adaptations, local endemism and striking biogeographical connections to remote parts of the world. Ages of the plant lineages and the timing of their radiations have frequently been debated but remain contentious as there are few estimates based on explicit models and fossil-calibrated molecular clocks. We used the plastid region maturaseK (matK) and a Caryophylloflora paleogenica fossil to infer the age of the genus Lychnis, and constructed a data set of three plastid (matK; a ribosomal protein S16 (rps16); and an intergenic spacer (psbE-petL)) and two nuclear (internal transcribed spacer (ITS) and a region spanning exon 18-24 in the second largest subunit of RNA polymerase II (RPB2)) loci for joint estimation of the species tree and divergence time of the African representatives. The time of divergence of the African high-altitude Lychnis was placed in the late Miocene to early Pliocene. A single speciation event was inferred in the early Pliocene; subsequent speciation took place sporadically from the late Pliocene to the middle Pleistocene. We provide further support for a Eurasian origin of the African 'sky islands' floral elements, which seem to have been recruited via dispersals at different times: some old, as in Lychnis, and others very recent. We show that dispersal and diversification within Africa play an important role in shaping these isolated plant communities.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1604
[Cu] Class update date: 160620
[Lr] Last revision date:160620
[Js] Journal subset:IM
[St] Status:In-Process
[do] DOI:10.1111/nph.13937

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[PMID]: 26354007
[Au] Autor:Dulya OV; Mikryukov VS
[Ad] Address:Institute of Plant and Animal Ecology, Ural Branch, Russian Academy of Sciences, 8th March Street 202, Ekaterinburg, 620144, Russia.
[Ti] Title:Genetic variation and selfing rate in Lychnis flos-cuculi along an industrial pollution gradient.
[So] Source:New Phytol;209(3):1083-95, 2016 Feb.
[Is] ISSN:1469-8137
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:We studied nine populations of a meadow mixed-mating plant Lychnis flos-cuculi growing in a gradient of copper smelter emissions. We hypothesize that metal tolerant populations in the polluted areas have experienced a loss of genetic variation and are more selfing than the populations from the unpolluted areas. One hundred and thirty-five parental plants and 1059 offspring were genotyped with six microsatellite markers. Selfing rates were assessed manually, with Rmes, Mltr and Colony2. Soil toxicity, population density and pollinators' activity were estimated in the studied areas. Populations from the heavily polluted area have experienced a strong founder effect. However, at present, they are characterized by high densities. A recent genetic explosion was registered for the population from the most polluted site, probably due to forest thinning under pollution effects. Selfing rates estimated with different approaches agreed well only for populations with high genetic variation; they comprised 0-0.23 and were similar between polluted and clean areas. Self-fertilization in L. flos-cuculi hardly represents a mechanism for the fixation of advantageous alleles and a barrier for gene flow from non-tolerant populations. The employment of different methods of selfing rate estimation in populations with low genetic variation appears to be necessary, though not a guarantee of reliable conclusions.
[Mh] MeSH terms primary: Air Pollution
Genetic Variation
Industry
Lychnis/genetics
Lychnis/physiology
Self-Fertilization/genetics
[Mh] MeSH terms secundary: Alleles
Flowers/genetics
Flowers/physiology
Genetics, Population
Geography
Heterozygote
Population Density
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1612
[Cu] Class update date: 161230
[Lr] Last revision date:161230
[Js] Journal subset:IM
[Da] Date of entry for processing:150911
[St] Status:MEDLINE
[do] DOI:10.1111/nph.13636

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[PMID]: 26513163
[Au] Autor:Raman G; Park S
[Ad] Address:Department of Life Sciences, Yeungnam University, Gyeongsan, Gyeongsan-buk, Republic of Korea.
[Ti] Title:Analysis of the Complete Chloroplast Genome of a Medicinal Plant, Dianthus superbus var. longicalyncinus, from a Comparative Genomics Perspective.
[So] Source:PLoS One;10(10):e0141329, 2015.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Dianthus superbus var. longicalycinus is an economically important traditional Chinese medicinal plant that is also used for ornamental purposes. In this study, D. superbus was compared to its closely related family of Caryophyllaceae chloroplast (cp) genomes such as Lychnis chalcedonica and Spinacia oleracea. D. superbus had the longest large single copy (LSC) region (82,805 bp), with some variations in the inverted repeat region A (IRA)/LSC regions. The IRs underwent both expansion and constriction during evolution of the Caryophyllaceae family; however, intense variations were not identified. The pseudogene ribosomal protein subunit S19 (rps19) was identified at the IRA/LSC junction, but was not present in the cp genome of other Caryophyllaceae family members. The translation initiation factor IF-1 (infA) and ribosomal protein subunit L23 (rpl23) genes were absent from the Dianthus cp genome. When the cp genome of Dianthus was compared with 31 other angiosperm lineages, the infA gene was found to have been lost in most members of rosids, solanales of asterids and Lychnis of Caryophyllales, whereas rpl23 gene loss or pseudogization had occurred exclusively in Caryophyllales. Nevertheless, the cp genome of Dianthus and Spinacia has two introns in the proteolytic subunit of ATP-dependent protease (clpP) gene, but Lychnis has lost introns from the clpP gene. Furthermore, phylogenetic analysis of individual protein-coding genes infA and rpl23 revealed that gene loss or pseudogenization occurred independently in the cp genome of Dianthus. Molecular phylogenetic analysis also demonstrated a sister relationship between Dianthus and Lychnis based on 78 protein-coding sequences. The results presented herein will contribute to studies of the evolution, molecular biology and genetic engineering of the medicinal and ornamental plant, D. superbus var. longicalycinus.
[Mh] MeSH terms primary: Dianthus/genetics
Genome, Chloroplast
Genomics
Plants, Medicinal/genetics
[Mh] MeSH terms secundary: Dianthus/classification
Gene Expression Regulation, Plant
Gene Order
Genes, Plant
Genetic Variation
Genomics/methods
Microsatellite Repeats
Open Reading Frames
Phylogeny
Plants, Medicinal/classification
RNA Editing
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1606
[Cu] Class update date: 170220
[Lr] Last revision date:170220
[Js] Journal subset:IM
[Da] Date of entry for processing:151030
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0141329

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[PMID]: 26463056
[Au] Autor:Plotnikov MB; Vasil'ev AS; Aliev OI; Zibareva LN
[Ad] Address:E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk, Russia. mbp2001@mail.ru.
[Ti] Title:Brattleboro Rats as the Model of Blood Hyperviscosity Syndrome for Testing Substances with Hemorheological Activity.
[So] Source:Bull Exp Biol Med;159(5):689-91, 2015 Sep.
[Is] ISSN:1573-8221
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Hyperviscosity syndrome was described in Brattleboro rats. The aim of this study was to investigate the possibility of Brattleboro rats using, as a test system for the study of agents with hemorheological activity. Under conditions of this model of high blood viscosity syndrome in Brattleboro rats, Lychnis chalcedonica L. extract (150 mg/kg) administered intragastrically for 10 days exhibited hemorheological activity by modulating macro- (plasma viscosity, fibrinogen concentration) and microrheological (erythrocyte aggregation and deformability parameters. Hence, Brattleboro rats are an adequate model of hyperviscosity syndrome that can be used for search and testing of substances with hemorheological activity.
[Mh] MeSH terms primary: Blood Viscosity/drug effects
Erythrocyte Deformability/drug effects
Erythrocytes/drug effects
Hematologic Agents/pharmacology
Plant Extracts/pharmacology
[Mh] MeSH terms secundary: Animals
Erythrocyte Aggregation/drug effects
Erythrocytes/metabolism
Erythrocytes/pathology
Fibrinogen/metabolism
Hematocrit
Male
Rats
Rats, Brattleboro
Rats, Wistar
Species Specificity
Syndrome
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Hematologic Agents); 0 (Plant Extracts); 0 (scarlet lightning extract); 9001-32-5 (Fibrinogen)
[Em] Entry month:1608
[Cu] Class update date: 160206
[Lr] Last revision date:160206
[Js] Journal subset:IM
[Da] Date of entry for processing:151015
[St] Status:MEDLINE
[do] DOI:10.1007/s10517-015-3048-9

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[PMID]: 26259831
[Au] Autor:Yoo RH; Zhao F; Lim S; Igori D; Lee SH; Moon JS
[Ad] Address:Biosystems and Bioengineering Program, University of Science and Technology (UST), Daejeon, 305-350, Republic of Korea.
[Ti] Title:The complete nucleotide sequence and genome organization of lychnis mottle virus.
[So] Source:Arch Virol;160(11):2891-4, 2015 Nov.
[Is] ISSN:1432-8798
[Cp] Country of publication:Austria
[La] Language:eng
[Ab] Abstract:The complete genomic sequence of lychnis mottle virus (LycMoV) from a Lychnis cognata plant was determined. LycMoV has a bipartite genome consisting of RNA1 (7,428 nt) and RNA2 (3,734 nt). Species in the family Secoviridae are demarcated based on their amino acid similarities in the protease-polymerase and coat protein. In LycMoV, these proteins share 90% and 63% sequence similarity, respectively, with the most closely related virus, strawberry latent ringspot virus, which is a member of the family Secoviridae but has not been assigned to a genus. Therefore, LycMoV is a tentative new virus of the family Secoviridae.
[Mh] MeSH terms primary: Genome, Viral
Lychnis/virology
Plant Diseases/virology
RNA Viruses/genetics
RNA Viruses/isolation & purification
[Mh] MeSH terms secundary: Base Sequence
Molecular Sequence Data
Open Reading Frames
Phylogeny
RNA Viruses/classification
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1605
[Cu] Class update date: 151106
[Lr] Last revision date:151106
[Js] Journal subset:IM
[Da] Date of entry for processing:150812
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-015-2501-4

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[PMID]: 26109770
[Au] Autor:Novozhilova E; Rybin V; Gorovoy P; Gavrilenko I; Doudkin R
[Ad] Address:Laboratory of Plant Chemotaxonomy, G.B. Elyakov Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
[Ti] Title:Phytoecdysteroids of the East Asian Caryophyllaceae.
[So] Source:Pharmacogn Mag;11(Suppl 1):S225-30, 2015 May.
[Is] ISSN:0973-1296
[Cp] Country of publication:India
[La] Language:eng
[Ab] Abstract:BACKGROUND: Occurrence of integristerone A (1), 20-hydroxyecdysone (2), ecdysone (3), 2-deoxy-20-hydroxyecdysone (4) has been analyzed in 64 species of the East Asian Caryophyllaceae. MATERIALS AND METHODS: Ecdysteroid content was determinate by high-performance liquid chromatography (HPLC). HPLC with a high-resolution mass spectrometry was performed on Shimadzu LCMS-IT-TOF (Japan) system equipped with a LC-20A Prominence liquid chromatograph, a photodiode array detector SPD-M20A and ion-trap/time-of-flight mass spectrometer. RESULTS: New sources of phytoecdysteroids: Melandrium sachalinense and Melandrium firmum have been revealed. It is the 1(st) time that two has been identified in M. sachalinense and M. firmum; 1 in the species: Lychnis fulgens, Silene repens, Silene foliosa, Silene stenophylla, Silene jenisseensis and M. sachalinense; 3 in Lychnis cognata; 4 in L. fulgens, S. stenophylla and S. jenisseensis (the tribe Lychnideae, the subfamily Caryophylloideae). Ecdysteroid-negative taxa are Spergularia rubra of the tribe Sperguleae; species of the genera Minuartia, Honckenya, Eremogone, Arenaria, Moehringia, Pseudostellaria, Fimbripetalum, Stellaria and Cerastium of the tribe Alsineae; Scleranthus annuus of the tribe Sclerantheae, as well as the East Asian representatives of the genera Gypsophila, Psammophiliela, Dianthus and Saponaria of the tribe Diantheae; Oberna and Agrostemma of the tribe Lychnideae. CONCLUSION: This investigation shows the most promising sources of ecdysteriods are species of genera Silene and Lychnis.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1506
[Cu] Class update date: 170220
[Lr] Last revision date:170220
[Da] Date of entry for processing:150626
[St] Status:PubMed-not-MEDLINE
[do] DOI:10.4103/0973-1296.157746

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[PMID]: 26085467
[Au] Autor:Ekroos J; Jakobsson A; Wideen J; Herbertsson L; Rundlöf M; Smith HG
[Ad] Address:Centre for Environmental and Climate Research, Lund University, Lund, Sweden. johan.ekroos@cec.lu.se.
[Ti] Title:Effects of landscape composition and configuration on pollination in a native herb: a field experiment.
[So] Source:Oecologia;179(2):509-18, 2015 Oct.
[Is] ISSN:1432-1939
[Cp] Country of publication:Germany
[La] Language:eng
[Ab] Abstract:Bumble bee abundance in agricultural landscapes is known to decrease with increasing distance from seminatural grasslands, but whether the pollination of bumble-bee-pollinated wild plants shows a similar pattern is less well known. In addition, the relative effects of landscape composition (landscape heterogeneity) and landscape configuration (distance from seminatural grassland) on wild plant pollination, and the interaction between these landscape effects, have not been studied using landscape-level replication. We performed a field experiment to disentangle these landscape effects on the pollination of a native herb, the sticky catchfly (Lychnis viscaria), while accounting for the proportion of oilseed rape across landscapes and the local abundance of bee forage flowers. We measured pollen limitation (the degree to which seed set is pollen-limited), seed set, and seed set stability using potted plants placed in landscapes that differed in heterogeneity (composition) and distance from seminatural grassland (configuration). Pollen limitation and seed set in individual plants did not respond to landscape composition, landscape configuration, or proportion of oilseed rape. Instead, seed set increased with increasing local bee forage flower cover. However, we found within-plant variability in pollen limitation and seed set to increase with increasing distance from seminatural pasture. Our results suggest that average within-plant levels of pollen limitation and seed set respond less swiftly than the within-plant variability in pollen limitation and seed set to changes in landscape configuration. Although landscape effects on pollination were less important than predicted, we conclude that landscape configuration and local habitat characteristics play larger roles than landscape composition in the pollination of L. viscaria.
[Mh] MeSH terms primary: Bees/physiology
Caryophyllaceae/physiology
Ecosystem
Pollination
[Mh] MeSH terms secundary: Agriculture
Animals
Behavior, Animal
Flowers/physiology
Pollen
Seeds
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Entry month:1605
[Cu] Class update date: 171010
[Lr] Last revision date:171010
[Js] Journal subset:IM
[Da] Date of entry for processing:150619
[St] Status:MEDLINE
[do] DOI:10.1007/s00442-015-3370-y


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