Database : MEDLINE
Search on : necatoriasis [Words]
References found : 343 [refine]
Displaying: 1 .. 10   in format [Detailed]

page 1 of 35 go to page                         

  1 / 343 MEDLINE  
              next record last record
select
to print
Photocopy
PubMed Central Full text
Full text

[PMID]: 23556013
[Au] Autor:Keiser J; Tritten L; Silbereisen A; Speich B; Adelfio R; Vargas M
[Ad] Address:Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland. jennifer.keiser@unibas.ch
[Ti] Title:Activity of oxantel pamoate monotherapy and combination chemotherapy against Trichuris muris and hookworms: revival of an old drug.
[So] Source:PLoS Negl Trop Dis;7(3):e2119, 2013.
[Is] ISSN:1935-2735
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: It is widely recognized that only a handful of drugs are available against soil-transmitted helminthiasis, all of which are characterized by a low efficacy against Trichuris trichiura, when administered as single doses. The re-evaluation of old, forgotten drugs is a promising strategy to identify alternative anthelminthic drug candidates or drug combinations. METHODOLOGY: We studied the activity of the veterinary drug oxantel pamoate against Trichuris muris, Ancylostoma ceylanicum and Necator americanus in vitro and in vivo. In addition, the dose-effect of oxantel pamoate combined with albendazole, mebendazole, levamisole, pyrantel pamoate and ivermectin was studied against T. muris in vitro and additive or synergistic combinations were followed up in vivo. PRINCIPAL FINDINGS: We calculated an ED50 of 4.7 mg/kg for oxantel pamoate against T. muris in mice. Combinations of oxantel pamoate with pyrantel pamoate behaved antagonistically in vitro (combination index (CI) = 2.53). Oxantel pamoate combined with levamisole, albendazole or ivermectin using ratios based on their ED50s revealed antagonistic effects in vivo (CI = 1.27, 1.90 and 1.27, respectively). A highly synergistic effect (CI = 0.15) was observed when oxantel pamoate-mebendazole was administered to T. muris-infected mice. Oxantel pamoate (10 mg/kg) lacked activity against Ancylostoma ceylanicum and Necator americanus in vivo. CONCLUSION/SIGNIFICANCE: Our study confirms the excellent trichuricidal properties of oxantel pamoate. Since the drug lacks activity against hookworms it is necessary to combine oxantel pamoate with a partner drug with anti-hookworm properties. Synergistic effects were observed for oxantel pamoate-mebendazole, hence this combination should be studied in more detail. Since, of the standard drugs, albendazole has the highest efficacy against hookworms, additional investigations on the combination effect of oxantel pamoate-albendazole should be launched.
[Mh] MeSH terms primary: Ancylostomiasis/drug therapy
Anthelmintics/administration & dosage
Necatoriasis/drug therapy
Pyrantel Pamoate/analogs & derivatives
Trichuriasis/drug therapy
[Mh] MeSH terms secundary: Ancylostoma/drug effects
Ancylostomiasis/parasitology
Animals
Anthelmintics/pharmacology
Disease Models, Animal
Drug Synergism
Drug Therapy, Combination/methods
Female
Mice
Mice, Inbred C57BL
Necator americanus/drug effects
Necatoriasis/parasitology
Parasitic Sensitivity Tests
Pyrantel Pamoate/administration & dosage
Pyrantel Pamoate/pharmacology
Treatment Outcome
Trichuriasis/parasitology
Trichuris/drug effects
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Anthelmintics); 81BK194Z5M (Pyrantel Pamoate); UPY1D732T0 (oxantel pamoate)
[Em] Entry month:1308
[Cu] Class update date: 131121
[Lr] Last revision date:131121
[Js] Journal subset:IM
[Da] Date of entry for processing:130404
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0002119

  2 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 22964044
[Au] Autor:Ungcharoensuk C; Putaporntip C; Pattanawong U; Jongwutiwes S
[Ad] Address:Molecular Biology of Malaria and Opportunistic Parasites Research Unit, Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
[Ti] Title:Sequence conservation in the Ancylostoma secreted protein-2 of Necator americanus (Na-ASP-2) from hookworm infected individuals in Thailand.
[So] Source:Infect Genet Evol;12(8):1926-32, 2012 Dec.
[Is] ISSN:1567-7257
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:The Ancylostoma secreted protein-2 of Necator americanus (Na-ASP-2) was one of the promising vaccine candidates against the most prevalent human hookworm species as adverse vaccine reaction has compromised further human vaccine trials. To elucidate the gene structure and the extent of sequence diversity, we determined the complete nucleotide sequence of the Na-asp-2 gene of individual larvae from 32 infected subjects living in 3 different endemic areas of Thailand. Sequence analysis revealed that the gene encoding Na-ASP-2 comprised 8 exons. Of 3 nucleotide substitutions in these exons, only one causes an amino acid change from leucine to methionine. A consensus conserved GT and AG at the 5' and the 3' boundaries of each intron was observed akin to those found in other eukaryotic genes. Introns of Na-asp-2 contained 23 nucleotide substitutions and 0-18 indels. The mean number of nucleotide substitutions per site (d) in introns was not significantly different from the mean number of synonymous substitutions per synonymous site (d(S)) in exons whereas d in introns was significantly exceeded d(N) (the mean number of nonsynonymous substitutions per nonsynonymous site) in exons (p<0.05), suggesting that introns and synonymous sites in exons may evolve at a similar rate whereas functional constraints at the amino acid could limit amino acid substitutions in Na-ASP-2. A recombination site was identified in an intron near the 3' portion of the gene. The positions of introns and the intron phases in the Na-asp-2 gene comparing with those in other pathogenesis-related-1 proteins of Loa loa, Onchocerca volvulus, Heterodera glycines, Caenorhabditis elegans and human were relatively conserved, suggesting evolutionary conservation of these genes. Sequence conservation in Na-ASP-2 may not compromise further vaccine design if adverse vaccine effects could be resolved whereas microheterogeneity in introns of this locus may be useful for population genetics analysis of N. americanus.
[Mh] MeSH terms primary: Conserved Sequence
Helminth Proteins/genetics
Necator americanus/genetics
Necatoriasis/parasitology
[Mh] MeSH terms secundary: Amino Acid Sequence
Animals
Base Sequence
Haplotypes
Helminth Proteins/chemistry
Humans
Introns
Larva
Molecular Sequence Data
Necator americanus/isolation & purification
Protein Conformation
Sequence Alignment
Thailand
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Helminth Proteins)
[Em] Entry month:1306
[Js] Journal subset:IM
[Da] Date of entry for processing:121031
[St] Status:MEDLINE

  3 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy

[PMID]: 23032277
[Au] Autor:Mudenda NB; Malone JB; Kearney MT; Mischler PD; Nieto Pdel M; McCarroll JC; Vounatsou P
[Ad] Address:Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA. nmuden1@tigers.lsu.edu
[Ti] Title:Modelling the ecological niche of hookworm in Brazil based on climate.
[So] Source:Geospat Health;6(3):S111-23, 2012 Sep.
[Is] ISSN:1970-7096
[Cp] Country of publication:Italy
[La] Language:eng
[Ab] Abstract:The distribution of hookworm in schistosomiasis-endemic areas in Brazil was mapped based on climate suitability. Known biological requirements of hookworm were fitted to data in a monthly long-term normal climate grid (18 x 18 km) using geographical information systems. Hookworm risk models were produced using the growing degree day (GDD) water budget (WB) concept. A moisture-adjusted model (MA-GDD) was developed based on accumulation of monthly temperatures above a base temperature of 15 C (below which there is no lifecycle progression of Necator americanus) conditional on concurrent monthly values (rain/potential, evapotranspiration) of over 0.4. A second model, designated the gradient index, was calculated based on the monthly accumulation of the product of GDD and monthly WB values (GDD x WB). Both parameters had a significant positive correlation to hookworm prevalence. In the northeastern part of Brazil (the Caatinga), low hookworm prevalence was due to low soil moisture content, while the low prevalence in southern Brazil was related to low mean monthly temperatures. Both environmental temperature and soil moisture content were found to be important parameters for predicting the prevalence of N. americanus.
[Mh] MeSH terms primary: Ancylostomatoidea
Climate
Geographic Information Systems
Necator americanus
Necatoriasis/epidemiology
[Mh] MeSH terms secundary: Animals
Brazil/epidemiology
Disease Models, Animal
Geographic Mapping
Humans
Models, Theoretical
Necatoriasis/transmission
Population Surveillance
Prevalence
Risk Assessment/methods
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Entry month:1303
[Js] Journal subset:IM
[Da] Date of entry for processing:121003
[St] Status:MEDLINE

  4 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Clinical Trials Registry
Full text

[PMID]: 22633322
[Au] Autor:Diemert DJ; Pinto AG; Freire J; Jariwala A; Santiago H; Hamilton RG; Periago MV; Loukas A; Tribolet L; Mulvenna J; Correa-Oliveira R; Hotez PJ; Bethony JM
[Ad] Address:Albert B. Sabin Vaccine Institute, Washington, DC 20037, USA. david.diemert@sabin.org
[Ti] Title:Generalized urticaria induced by the Na-ASP-2 hookworm vaccine: implications for the development of vaccines against helminths.
[So] Source:J Allergy Clin Immunol;130(1):169-76.e6, 2012 Jul.
[Is] ISSN:1097-6825
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Necator americanus Ancylostoma-secreted protein 2 (Na-ASP-2) is secreted by infective hookworm larvae on entry into human hosts. Vaccination of laboratory animals with recombinant Na-ASP-2 provides significant protection against challenge infections. In endemic areas antibodies to Na-ASP-2 are associated with reduced risk of heavy N americanus infections. OBJECTIVE: To assess the safety and immunogenicity of recombinant Na-ASP-2 adjuvanted with Alhydrogel in healthy Brazilian adults previously infected with N americanus. METHODS: Participants were randomized to receive Na-ASP-2 or hepatitis B vaccine. Major IgG and IgE epitopes of the Na-ASP-2 molecule were mapped by using sera from these same subjects. Seroepidemiologic studies in adults and children residing in hookworm-endemic areas were conducted to assess the prevalence of IgE responses to Na-ASP-2. RESULTS: Vaccination with a single dose of Na-ASP-2 resulted in generalized urticarial reactions in several volunteers. These reactions were associated with pre-existing Na-ASP-2-specific IgE likely induced by previous hookworm infection. Surveys revealed that a significant proportion of the population in hookworm-endemic areas had increased levels of IgE to Na-ASP-2. Epitope mapping demonstrated sites on the Na-ASP-2 molecule that are uniquely or jointly recognized by IgG and IgE antibodies. CONCLUSION: Infection with N americanus induces increased levels of total and specific IgE to Na-ASP-2 that result in generalized urticaria on vaccination with recombinant Na-ASP-2. These data advance knowledge of vaccine development for helminths given their propensity to induce strong T(H)2 responses. Study data highlight the important differences between the immune responses to natural helminth infection and to vaccination with a recombinant helminth antigen.
[Mh] MeSH terms primary: Antigens, Helminth/adverse effects
Helminth Proteins/adverse effects
Necator americanus/immunology
Necatoriasis/prevention & control
Urticaria/epidemiology
Vaccines, Synthetic/adverse effects
[Mh] MeSH terms secundary: Adolescent
Adult
Animals
Antigens, Helminth/administration & dosage
Antigens, Helminth/immunology
Brazil/epidemiology
Epitope Mapping
Female
Helminth Proteins/administration & dosage
Helminth Proteins/immunology
Humans
Immunoglobulin E/blood
Immunoglobulin G/blood
Male
Middle Aged
Necatoriasis/epidemiology
Necatoriasis/immunology
Seroepidemiologic Studies
Treatment Outcome
Urticaria/etiology
Vaccination/adverse effects
Vaccines, Synthetic/administration & dosage
Vaccines, Synthetic/immunology
Young Adult
[Pt] Publication type:CLINICAL TRIAL, PHASE I; JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Antigens, Helminth); 0 (Helminth Proteins); 0 (Immunoglobulin G); 0 (Vaccines, Synthetic); 37341-29-0 (Immunoglobulin E)
[Em] Entry month:1209
[Js] Journal subset:AIM; IM
[Da] Date of entry for processing:120629
[Cl] Clinical Trial:ClinicalTrial
[St] Status:MEDLINE
[do] DOI:10.1016/j.jaci.2012.04.027

  5 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
Full text

[PMID]: 22024448
[Au] Autor:Xue J; Zhan B; Guo J; He N; Qiang HQ; Hotez P; Xiao SH
[Ad] Address:National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Key Laboratory of Parasite and Vector Biology, MOH, WHO Collaborating Centre for Malaria, Schistosomiasis, and Filariasis, 207 Rui Jin Er Lu, Shanghai 200025, People's Republic of China.
[Ti] Title:Acquired hookworm immunity in the golden hamster (Mesocricetus auratus) elicited by living Necator americanus third-stage infective larvae.
[So] Source:Exp Parasitol;130(1):6-12, 2012 Jan.
[Is] ISSN:1090-2449
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The aim of the study is to demonstrate and understand the acquired immunity in golden hamsters (Mesocricetus auratus) elicited by primary Necator americanus infective third-stage larvae (L3) infection. Hamsters infected with 150 L3 for 1, 2, 3, 6 and 10 weeks, were challenged with the same number of L3 and sacrificed 25 days post challenge. The primarily infected hamsters exhibited 99-100% protection against subsequent L3 challenge compared to un-infected naive hamsters. The acquired immunity was developed as early as 1 week post L3 infection and lasted up to 10 weeks. Similar protective immunity was obtained in hamsters infected with N. americanus L3 and then treated orally with a single of 100mg/kg albendazole, followed by challenge with N. americanus L3 4 and 8 weeks post-treatment. The infected hamsters exhibited a rise in IgG antibodies against L3 and juvenile adult worm antigens. Histological examination showed that challenging L3 were trapped in the skin of primarily infected hamsters and surrounded or infiltrated by different inflammatory cells. The trapped L3 were damaged and dead followed by the formation of granulomas encasing dead worms. The results demonstrate that hamsters primarily infected with N. americanus L3 develop acquired immunity against re-infection.
[Mh] MeSH terms primary: Adaptive Immunity
Necator americanus/immunology
Necatoriasis/immunology
[Mh] MeSH terms secundary: Albendazole/therapeutic use
Animals
Antibodies, Helminth/biosynthesis
Antibodies, Helminth/blood
Anticestodal Agents/therapeutic use
Antigens, Helminth/immunology
Cricetinae
Disease Models, Animal
Immunoglobulin G/blood
Larva/immunology
Male
Mesocricetus
Necatoriasis/drug therapy
Necatoriasis/pathology
Random Allocation
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Antibodies, Helminth); 0 (Anticestodal Agents); 0 (Antigens, Helminth); 0 (Immunoglobulin G); F4216019LN (Albendazole)
[Em] Entry month:1201
[Cu] Class update date: 131121
[Lr] Last revision date:131121
[Js] Journal subset:IM
[Da] Date of entry for processing:111205
[St] Status:MEDLINE
[do] DOI:10.1016/j.exppara.2011.10.007

  6 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
PubMed Central Full text
Full text

[PMID]: 22064562
[Au] Autor:Schneider B; Jariwala AR; Periago MV; Gazzinelli MF; Bose SN; Hotez PJ; Diemert DJ; Bethony JM
[Ad] Address:Department of Microbiology, Immunology and Tropical Medicine, The George Washington University Medical Center; Washington, DC USA.
[Ti] Title:A history of hookworm vaccine development.
[So] Source:Hum Vaccin;7(11):1234-44, 2011 Nov.
[Is] ISSN:1554-8619
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:The human hookworms Necator americanus and Ancylostoma duodenale remain among the most common infections of humans in areas of rural poverty in the developing regions of the world, with an estimated 1 billion people infected with one or more of these parasites. Herein, we review the nearly 100 years of research, development, animal testing, and fieldwork that have led to our current progress in recombinant hookworm vaccines. We begin with the identification of hookworm at the start of the 20th century in Southern US, then discuss the progress in developed countries to eliminate human hookworm infection, and then the industrial development and field use in the 1970s a canine hookworm vaccine(Ancylostoma caninum), and finally our progress to date in the development and clinical testing of an array of recombinant antigens to prevent human hookworm disease from N. americanus infection. Special attention is given to the challenges faced in the development of a vaccine against a blood-feeding nematode, including the epidemiology of infection (high prevalence of infection), pathogenesis (chronic infection that increases with the age of the host), and a robust immune response that fails to confer the protection in the host and a concomitant absence of correlates of protection by a successful vaccine could be developed and tested. Finally, we provide the optimal and acceptable profiles of a human hookworm vaccine, including the proposed indication, target population, and route of administration, as developed by the Human Hookworm Vaccine Initiative, the only group currently working on vaccines targeting this parasite.
[Mh] MeSH terms primary: Ancylostomatoidea/immunology
Ancylostomiasis/prevention & control
Necatoriasis/prevention & control
Vaccines, Synthetic/immunology
[Mh] MeSH terms secundary: Ancylostoma/immunology
Ancylostomatoidea/genetics
Ancylostomiasis/immunology
Ancylostomiasis/veterinary
Animals
Antigens, Helminth/genetics
Antigens, Helminth/immunology
Dog Diseases/immunology
Dog Diseases/prevention & control
Dogs
Humans
Necator americanus/immunology
Necatoriasis/immunology
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Nm] Name of substance:0 (Antigens, Helminth); 0 (Vaccines, Synthetic)
[Em] Entry month:1204
[Cu] Class update date: 131016
[Lr] Last revision date:131016
[Js] Journal subset:IM
[Da] Date of entry for processing:111229
[St] Status:MEDLINE
[do] DOI:10.4161/hv.7.11.18443

  7 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
PubMed Central Full text
Full text

[PMID]: 21949691
[Au] Autor:McSorley HJ; Gaze S; Daveson J; Jones D; Anderson RP; Clouston A; Ruyssers NE; Speare R; McCarthy JS; Engwerda CR; Croese J; Loukas A
[Ad] Address:Queensland Tropical Health Alliance, James Cook University, Cairns, Queensland, Australia.
[Ti] Title:Suppression of inflammatory immune responses in celiac disease by experimental hookworm infection.
[So] Source:PLoS One;6(9):e24092, 2011.
[Is] ISSN:1932-6203
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:We present immunological data from two clinical trials where the effect of experimental human hookworm (Necator americanus) infection on the pathology of celiac disease was evaluated. We found that basal production of Interferon- (IFN-)γ and Interleukin- (IL-)17A from duodenal biopsy culture was suppressed in hookworm-infected participants compared to uninfected controls. Increased levels of CD4+CD25+Foxp3+ cells in the circulation and mucosa are associated with active celiac disease. We show that this accumulation also occurs during a short-term (1 week) oral gluten challenge, and that hookworm infection suppressed the increase of circulating CD4+CD25+Foxp3+ cells during this challenge period. When duodenal biopsies from hookworm-infected participants were restimulated with the immunodominant gliadin peptide QE65, robust production of IL-2, IFN-γ and IL-17A was detected, even prior to gluten challenge while participants were strictly adhering to a gluten-free diet. Intriguingly, IL-5 was produced only after hookworm infection in response to QE65. Thus we hypothesise that hookworm-induced TH2 and IL-10 cross-regulation of the TH1/TH17 inflammatory response may be responsible for the suppression of these responses during experimental hookworm infection.
[Mh] MeSH terms primary: Celiac Disease/immunology
Duodenum/immunology
Necator americanus/immunology
Necatoriasis/immunology
[Mh] MeSH terms secundary: Animals
Antigens, CD4/immunology
Antigens, CD4/metabolism
Biopsy
Celiac Disease/parasitology
Celiac Disease/pathology
Cells, Cultured
Clinical Trials as Topic
Duodenum/metabolism
Duodenum/pathology
Forkhead Transcription Factors/immunology
Forkhead Transcription Factors/metabolism
Gliadin/immunology
Host-Parasite Interactions/immunology
Humans
Immunohistochemistry
Interferon-gamma/immunology
Interferon-gamma/metabolism
Interleukin-17/immunology
Interleukin-17/metabolism
Interleukin-2/immunology
Interleukin-2/metabolism
Interleukin-2 Receptor alpha Subunit/immunology
Interleukin-2 Receptor alpha Subunit/metabolism
Interleukin-5/immunology
Interleukin-5/metabolism
Intestinal Mucosa/immunology
Intestinal Mucosa/metabolism
Leukocytes, Mononuclear/cytology
Leukocytes, Mononuclear/immunology
Leukocytes, Mononuclear/metabolism
Necator americanus/physiology
Necatoriasis/parasitology
Necatoriasis/pathology
Time Factors
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Antigens, CD4); 0 (FOXP3 protein, human); 0 (Forkhead Transcription Factors); 0 (Interleukin-17); 0 (Interleukin-2); 0 (Interleukin-2 Receptor alpha Subunit); 0 (Interleukin-5); 82115-62-6 (Interferon-gamma); 9007-90-3 (Gliadin)
[Em] Entry month:1203
[Cu] Class update date: 131016
[Lr] Last revision date:131016
[Js] Journal subset:IM
[Da] Date of entry for processing:110928
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0024092

  8 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
SciELO Brazil full text

[PMID]: 21915471
[Au] Autor:Andrade-Filho Jde S
[Ti] Title:Analogies in medicine: the American killer.
[So] Source:Rev Inst Med Trop Sao Paulo;53(4):339, 2011 Jul-Aug.
[Is] ISSN:1678-9946
[Cp] Country of publication:Brazil
[La] Language:eng
[Mh] MeSH terms primary: Ancylostomiasis/parasitology
Metaphor
Necatoriasis/parasitology
[Mh] MeSH terms secundary: Ancylostoma/pathogenicity
Animals
Humans
Necator americanus/pathogenicity
[Pt] Publication type:LETTER
[Em] Entry month:1202
[Cu] Class update date: 120404
[Lr] Last revision date:120404
[Js] Journal subset:IM
[Da] Date of entry for processing:110914
[St] Status:MEDLINE

  9 / 343 MEDLINE  
              first record previous record next record last record
select
to print
Photocopy
PubMed Central Full text
Full text

[PMID]: 21909439
[Au] Autor:Geiger SM; Alexander ND; Fujiwara RT; Brooker S; Cundill B; Diemert DJ; Correa-Oliveira R; Bethony JM
[Ad] Address:Centro de Pesquisas Ren Rachou, Fundao Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil. stefan@cpqrr.fiocruz.br
[Ti] Title:Necator americanus and helminth co-infections: further down-modulation of hookworm-specific type 1 immune responses.
[So] Source:PLoS Negl Trop Dis;5(9):e1280, 2011 Sep.
[Is] ISSN:1935-2735
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Helminth co-infection in humans is common in tropical regions of the world where transmission of soil-transmitted helminths such as Ascaris lumbricoides, Trichuris trichiura, and the hookworms Necator americanus and Ancylostoma duodenale as well as other helminths such as Schistosoma mansoni often occur simultaneously. METHODOLOGY: We investigated whether co-infection with another helminth(s) altered the human immune response to crude antigen extracts from either different stages of N. americanus infection (infective third stage or adult) or different crude antigen extract preparations (adult somatic and adult excretory/secretory). Using these antigens, we compared the cellular and humoral immune responses of individuals mono-infected with hookworm (N. americanus) and individuals co-infected with hookworm and other helminth infections, namely co-infection with either A. lumbricoides, Schistosoma mansoni, or both. Immunological variables were compared between hookworm infection group (mono- versus co-infected) by bootstrap, and principal component analysis (PCA) was used as a data reduction method. CONCLUSIONS: Contrary to several animal studies of helminth co-infection, we found that co-infected individuals had a further downmodulated Th1 cytokine response (e.g., reduced INF-γ), accompanied by a significant increase in the hookworm-specific humoral immune response (e.g. higher levels of IgE or IgG4 to crude antigen extracts) compared with mono- infected individuals. Neither of these changes was associated with a reduction of hookworm infection intensity in helminth co-infected individuals. From the standpoint of hookworm vaccine development, these results are relevant; i.e., the specific immune response to hookworm vaccine antigens might be altered by infection with another helminth.
[Mh] MeSH terms primary: Antibodies, Helminth/blood
Ascariasis/immunology
Coinfection/immunology
Lymphocytes/immunology
Necator americanus/immunology
Necatoriasis/immunology
Schistosomiasis mansoni/immunology
[Mh] MeSH terms secundary: Adolescent
Adult
Aged
Aged, 80 and over
Animals
Antigens, Helminth/diagnostic use
Ascariasis/parasitology
Ascaris lumbricoides/immunology
Child
Coinfection/parasitology
Female
Humans
Immunoglobulin E/blood
Immunoglobulin G/blood
Interferon-gamma/secretion
Male
Middle Aged
Necatoriasis/parasitology
Schistosoma mansoni/immunology
Schistosomiasis mansoni/parasitology
Young Adult
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (Antibodies, Helminth); 0 (Antigens, Helminth); 0 (Immunoglobulin G); 37341-29-0 (Immunoglobulin E); 82115-62-6 (Interferon-gamma)
[Em] Entry month:1201
[Cu] Class update date: 131016
[Lr] Last revision date:131016
[Js] Journal subset:IM
[Da] Date of entry for processing:110912
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pntd.0001280

  10 / 343 MEDLINE  
              first record previous record
select
to print
Photocopy
PubMed Central Full text
Full text

[PMID]: 21292911
[Au] Autor:Basuni M; Muhi J; Othman N; Verweij JJ; Ahmad M; Miswan N; Rahumatullah A; Aziz FA; Zainudin NS; Noordin R
[Ad] Address:Institute for Research in Molecular Medicine, Universiti Sains Malaysia (USM), Penang, Malaysia. madihah_basuni@yahoo.com
[Ti] Title:A pentaplex real-time polymerase chain reaction assay for detection of four species of soil-transmitted helminths.
[So] Source:Am J Trop Med Hyg;84(2):338-43, 2011 Feb.
[Is] ISSN:1476-1645
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Soil-transmitted helminth infections remain a major public health burden in low- and middle-income countries. The traditional diagnosis by microscopic examination of fecal samples is insensitive and time-consuming. In this study, a pentaplex real-time polymerase chain reaction (PCR) was evaluated for the simultaneous detection of Ancylostoma, Necator americanus, Ascaris lumbricoides, and Strongyloides stercoralis. The results were compared with those obtained by conventional parasitological diagnostic methods. Real-time PCR was positive in 48 of 77 samples (62.3%) and microscopic examination was positive in six samples (7.8%) only (P < 0.05). In conclusion, the real-time PCR assay described in this study provides a specific and sensitive diagnostic tool for the detection of these four helminth species in epidemiological studies and monitoring of treatment programs.
[Mh] MeSH terms primary: Ancylostoma
Ancylostomiasis/diagnosis
Ascariasis/diagnosis
Ascaris lumbricoides
Necator americanus
Necatoriasis/diagnosis
Reverse Transcriptase Polymerase Chain Reaction/methods
Strongyloides stercoralis
Strongyloidiasis/diagnosis
[Mh] MeSH terms secundary: Ancylostoma/genetics
Animals
Ascaris lumbricoides/genetics
DNA, Protozoan/genetics
Feces/parasitology
Necator americanus/genetics
Parasite Egg Count
Strongyloides stercoralis/genetics
[Pt] Publication type:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Name of substance:0 (DNA, Protozoan)
[Em] Entry month:1103
[Cu] Class update date: 131018
[Lr] Last revision date:131018
[Js] Journal subset:AIM; IM
[Da] Date of entry for processing:110204
[St] Status:MEDLINE
[do] DOI:10.4269/ajtmh.2011.10-0499


page 1 of 35 go to page                         
   


Refine the search
  Database : MEDLINE Advanced form   

    Search in field  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/PAHO/WHO - Latin American and Caribbean Center on Health Sciences Information