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[PMID]: 29431947
[Au] Autor:Lapko IV; Kiryakov VA; Pavlovskaya NA; Oshkoderov OA; Klimkina KV
[Ti] Title:[Choice of informative laboratory biomarkers for the early identification of changes in neurohumoral regulation and carbohydrate exchange in workers of the mining and mechanical engineering industry].
[So] Source:Gig Sanit;95(11):1061-5, 2016.
[Is] ISSN:0016-9900
[Cp] Country of publication:Russia (Federation)
[La] Language:rus
[Ab] Abstract:The diagnostic significance of hormones and integral indices of pituitary-adrenal, pituitary-thyroid and pituitary-gonadal system and carbohydrate metabolism (ACTH (corticotropin), aldosterone, cortisol, TSH (thyroid-stimulating hormone), free triiodothyronine (fT3), free thyroxine (fT4), luteinizing hormone (LH), follicle-stimulating hormone (FSH), total and free testosterone, insulin, integral pituitary-adrenal index (IPAI), the pituitary-thyroid index (PTI), indices of carbohydrate metabolism (Caro and HOMA-IR) was studied for the early diagnostics of disorders of neurohumoral regulation in workers of mining and mechanical engineering industries. The most informative indices, permitting to identify disorders of carbohydrate metabolism are established to be indices of insulin resistance (index Caro and index NOMA-IR) and the determination of insulin in serum. For the identification of changes in pituitary adrenal, pituitary-thyroid and pituitary-gonadal system in patients with vibration disease, sensory-neural hearing loss, comorbidity indexes IGNI, ITI, concentrations of LH and total testosterone are of the most diagnostically significance.
[Mh] MeSH terms primary: Adrenocorticotropic Hormone/blood
Follicle Stimulating Hormone/blood
Insulin/blood
Occupational Diseases
Thyrotropin/blood
[Mh] MeSH terms secundary: Adult
Biomarkers/blood
Carbohydrate Metabolism/physiology
Extraction and Processing Industry/methods
Extraction and Processing Industry/standards
Female
Humans
Hypothalamo-Hypophyseal System/metabolism
Male
Middle Aged
Occupational Diseases/blood
Occupational Diseases/diagnosis
Occupational Diseases/etiology
Occupational Diseases/prevention & control
Occupational Health
Pituitary-Adrenal System/metabolism
Reproducibility of Results
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Biomarkers); 0 (Insulin); 9002-60-2 (Adrenocorticotropic Hormone); 9002-68-0 (Follicle Stimulating Hormone); 9002-71-5 (Thyrotropin)
[Em] Entry month:1803
[Cu] Class update date: 180309
[Lr] Last revision date:180309
[Js] Journal subset:IM
[Da] Date of entry for processing:180213
[St] Status:MEDLINE

  2 / 15998 MEDLINE  
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[PMID]: 29267508
[Au] Autor:Wu J; Wang Y; Liu G; Jia Y; Yang J; Shi J; Dong J; Wei J; Liu X
[Ad] Address:College of Clinical Medicine, Ningxia Medical University, Yinchuan, Ningxia, China.
[Ti] Title:Characterization of air-liquid interface culture of A549 alveolar epithelial cells.
[So] Source:Braz J Med Biol Res;51(2):e6950, 2017 Dec 11.
[Is] ISSN:1414-431X
[Cp] Country of publication:Brazil
[La] Language:eng
[Ab] Abstract:Alveolar epithelia play an essential role in maintaining the integrity and homeostasis of lungs, in which alveolar epithelial type II cells (AECII) are a cell type with stem cell potential for epithelial injury repair and regeneration. However, mechanisms behind the physiological and pathological roles of alveolar epithelia in human lungs remain largely unknown, partially owing to the difficulty of isolation and culture of primary human AECII cells. In the present study, we aimed to characterize alveolar epithelia generated from A549 lung adenocarcinoma cells that were cultured in an air-liquid interface (ALI) state. Morphological analysis demonstrated that A549 cells could reconstitute epithelial layers in ALI cultures as evaluated by histochemistry staining and electronic microscopy. Immunofluorescent staining further revealed an expression of alveolar epithelial type I cell (AECI) markers aquaporin-5 protein (AQP-5), and AECII cell marker surfactant protein C (SPC) in subpopulations of ALI cultured cells. Importantly, molecular analysis further revealed the expression of AQP-5, SPC, thyroid transcription factor-1, zonula occludens-1 and Mucin 5B in A549 ALI cultures as determined by both immunoblotting and quantitative RT-PCR assay. These results suggest that the ALI culture of A549 cells can partially mimic the property of alveolar epithelia, which may be a feasible and alternative model for investigating roles and mechanisms of alveolar epithelia in vitro.
[Mh] MeSH terms primary: A549 Cells/physiology
Alveolar Epithelial Cells/physiology
Cell Culture Techniques/methods
Culture Media, Conditioned
[Mh] MeSH terms secundary: Analysis of Variance
Aquaporin 5/analysis
Cell Count
Humans
Immunoblotting
Microscopy, Electron, Scanning
Mucin-5B/analysis
Pulmonary Surfactant-Associated Protein C/analysis
Real-Time Polymerase Chain Reaction
Reference Values
Reproducibility of Results
Thyroid Nuclear Factor 1/analysis
Time Factors
Zonula Occludens-1 Protein/analysis
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Aquaporin 5); 0 (Culture Media, Conditioned); 0 (Mucin-5B); 0 (NKX2-1 protein, human); 0 (Pulmonary Surfactant-Associated Protein C); 0 (Thyroid Nuclear Factor 1); 0 (Zonula Occludens-1 Protein)
[Em] Entry month:1803
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[Js] Journal subset:IM
[Da] Date of entry for processing:171222
[St] Status:MEDLINE

  3 / 15998 MEDLINE  
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[PMID]: 29275552
[Au] Autor:Zhang MZ; Li BX; Zhao R; Guan DW; Zhang GH; Wu X; Zhu BL; Li RB
[Ad] Address:Department of Forensic Pathology, School of Forensic Medicine, China Medical University, Shenyang 110122, China.
[Ti] Title:[Forensic Analysis of 6 Cases of Sudden Death due to Hyperthyroid Heart Disease].
[So] Source:Fa Yi Xue Za Zhi;33(5):482-485, 2017 Oct.
[Is] ISSN:1004-5619
[Cp] Country of publication:China
[La] Language:chi
[Ab] Abstract:OBJECTIVES: To analyse the cases of sudden death due to hyperthyroid heart disease, and explore the general information of deaths and the forensic pathological characteristics to provide reference evidence for forensic identification of such cases. METHODS: Six cases of sudden death due to hyperthyroid heart disease between 2001 and 2016 were selected from School of Forensic Medicine, China Medical University. The general information (gender and age), clinical manifestations, medical history, anatomical and histopathological findings, biochemical parameters and cause of death were analysed retrospectively. RESULTS: Most of the 6 patients had definite history of hyperthyroidism, and they all showed certain degrees of symptoms of cardiovascular disease; had obvious incentive factors of death; histopathological examination of thyroid conformed to the performances of diffuse toxic goiter; with increase of cardiac weight, dilatation of cardiac chambers, myocardial hypertrophy and focal necrosis; postmortem biochemical analyses of pericardial fluid could be used as an additional method for diagnostic of sudden death due to hyperthyroid heart disease. CONCLUSIONS: The identification of death due to hyperthyroid heart disease should be based on the clinical history and the results of autopsy, histopathological examination, postmortem toxicology tests. The postmortem biochemical detection of thyroid and cardiac function should be performed if necessary.
[Pt] Publication type:ENGLISH ABSTRACT; JOURNAL ARTICLE
[Em] Entry month:1712
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[St] Status:In-Process
[do] DOI:10.3969/j.issn.1004-5619.2017.05.005

  4 / 15998 MEDLINE  
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[PMID]: 28460140
[Au] Autor:Samuels MH; Kolobova I; Antosik M; Niederhausen M; Purnell JQ; Schuff KG
[Ad] Address:Division of Endocrinology, Diabetes and Clinical Nutrition, Oregon Health & Science University, Portland, Oregon 97239.
[Ti] Title:Thyroid Function Variation in the Normal Range, Energy Expenditure, and Body Composition in L-T4-Treated Subjects.
[So] Source:J Clin Endocrinol Metab;102(7):2533-2542, 2017 Jul 01.
[Is] ISSN:1945-7197
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Purpose: It is not clear whether upper limits of the thyrotropin (TSH) reference range should be lowered. This debate can be better informed by investigation of whether variations in thyroid function within the reference range have clinical effects. Thyroid hormone plays a critical role in determining energy expenditure, body mass, and body composition, and therefore clinically relevant variations in these parameters may occur across the normal range of thyroid function. Methods: This was a cross-sectional study of 140 otherwise healthy hypothyroid subjects receiving chronic replacement therapy with levothyroxine (L-T4) who had TSH levels across the full span of the laboratory reference range (0.34 to 5.6 mU/L). Subjects underwent detailed tests of energy expenditure (total and resting energy expenditure, thermic effect of food, physical activity energy expenditure), substrate oxidation, diet intake, and body composition. Results: Subjects with low-normal (≤2.5 mU/L) and high-normal (>2.5 mU/L) TSH levels did not differ in any of the outcome measures. However, across the entire group, serum free triiodothyronine (fT3) levels were directly correlated with resting energy expenditure, body mass index (BMI), body fat mass, and visceral fat mass, with clinically relevant variations in these outcomes. Conclusions: Variations in thyroid function within the laboratory reference range have clinically relevant correlations with resting energy expenditure, BMI, and body composition in L-T4-treated subjects. However, salutary effects of higher fT3 levels on energy expenditure may be counteracted by deleterious effects on body weight and composition. Further studies are needed before these outcomes should be used as a basis for altering L-T4 doses in L-T4-treated subjects.
[Mh] MeSH terms primary: Body Composition/drug effects
Energy Metabolism/drug effects
Hypothyroidism/diagnosis
Hypothyroidism/drug therapy
Thyroxine/administration & dosage
[Mh] MeSH terms secundary: Absorptiometry, Photon/methods
Adult
Aged
Anthropometry
Cross-Sectional Studies
Exercise/physiology
Female
Humans
Male
Middle Aged
Reference Values
Severity of Illness Index
Thyroid Function Tests
Young Adult
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:Q51BO43MG4 (Thyroxine)
[Em] Entry month:1710
[Cu] Class update date: 180308
[Lr] Last revision date:180308
[Js] Journal subset:AIM; IM
[Da] Date of entry for processing:170502
[St] Status:MEDLINE
[do] DOI:10.1210/jc.2017-00224

  5 / 15998 MEDLINE  
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[PMID]: 29512190
[Au] Autor:Ercan M; Firat Oguz E; Akbulut ED; Yilmaz M; Turhan T
[Ad] Address:Faculty of Medicine, Department of Biochemistry, Bozok University, Yozgat, Turkey.
[Ti] Title:Comparison of the effect of gel used in two different serum separator tubes for thyroid function tests.
[So] Source:J Clin Lab Anal;, 2018 Mar 07.
[Is] ISSN:1098-2825
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:BACKGROUND: Selection and verification of blood collection tubes is an important preanalytical issue in clinical laboratories. Today, gel tubes are commonly used with many advantages, although they are known to cause interference in immunoassay methods. In this study, we aimed to compare SSTs of two different suppliers (Ayset clot activator & Gel and Becton Dickinson (BD) Vacutainer SST II advance) with reference tubes and evaluate the effect of storage time in terms of commonly used endocrine tests such as thyroid-stimulating hormone (TSH), free thyroxine (fT4), and free triiodothyronine (fT3). METHODS: Fifty-five volunteers were included in the study. Samples were taken into three different tubes and analyzed for serum TSH, fT4, and fT3 on Architect ci8200 Immunoassay System. Clinical decision levels were estimated using total allowable error (TEa). RESULTS: No difference was found between tubes in terms of TSH, fT3, and fT4 levels. From a statistical standpoint, TSH and fT4 levels were no longer stable during 24, 48, and 72 hours storage time periods. However, their variations were not clinically significant. CONCLUSION: Ayset clot activator & Gel tubes and BD Vacutainer SST II advance tubes have comparable results with glass tube in terms of TSH, fT3, and fT4 levels on Architect ci8200 Immunoassay Systems. From a clinical standpoint, serum TSH, fT4, and fT3 concentrations may be considered as stable when storing these tubes over 72 hours.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[St] Status:Publisher
[do] DOI:10.1002/jcla.22427

  6 / 15998 MEDLINE  
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[PMID]: 29369334
[Au] Autor:Fisher SB; Perrier ND
[Ad] Address:Fellow, Department of Surgical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX.
[Ti] Title:The incidental thyroid nodule.
[So] Source:CA Cancer J Clin;68(2):97-105, 2018 Mar.
[Is] ISSN:1542-4863
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Incidental thyroid nodules that are found on an imaging study performed for reasons other than thyroid pathology represent a common scenario encountered by health care providers. The initial workup for these nodules comprises a thorough history and physical examination, thyroid function tests, a dedicated thyroid ultrasound, and fine-needle aspiration of any suspicious lesions. Management ranges from observation and reassurance to surgical resection and depends on the cytologic diagnosis. In cases of cytologically indeterminate or discordant nodules, surgical excision (lobectomy) offers a definitive diagnosis, although molecular testing or a reasonable period of observation may be useful as less invasive adjuncts. CA Cancer J Clin 2018;68:97-105. © 2018 American Cancer Society.
[Pt] Publication type:JOURNAL ARTICLE; REVIEW
[Em] Entry month:1801
[Cu] Class update date: 180307
[Lr] Last revision date:180307
[St] Status:In-Data-Review
[do] DOI:10.3322/caac.21447

  7 / 15998 MEDLINE  
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[PMID]: 29329570
[Au] Autor:Erdei A; Steiber Z; Molnar C; Berenyi E; Nagy EV
[Ad] Address:Division of Endocrinology, Department of Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei krt 98, Debrecen, 4032, Hungary. erdeianm@gmail.com.
[Ti] Title:Exophthalmos in a young woman with no graves' disease - a case report of IgG4-related orbitopathy.
[So] Source:BMC Ophthalmol;18(1):5, 2018 Jan 12.
[Is] ISSN:1471-2415
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:BACKGROUND: Immunoglobulin G4-related disease (IgG4-rd) is characterized by lymphoplasmacytic infiltration and tissue fibrosis. Orbital manifestations of IgG4-rd may include unilateral or bilateral proptosis, cicatricial extraocular muscle myopathy, orbital inflammation and pain which may mimic ophthalmic Graves' disease. CASE PRESENTATION: A 25-year-old woman has been referred to the endocrinology clinic, 4 months after delivery, with suspected Graves' orbitopathy. She has had bronchial asthma and recurrent skin rashes of unknown aetiology for the last 10 years and was treated for dacryoadenitis with steroid containing eye drops 5 years ago. During pregnancy she developed eyelid swelling. After delivery, eyelid redness and retrobulbar pain evolved. Proptosis was demonstrated by Hertel's exophthalmometry. Orbital magnetic resonance imaging showed enlarged lateral and superior rectus muscles in both orbits. Thyroid function tests were in the normal range and no thyroid stimulating hormone (TSH) receptor autoantibodies were present. The eye muscle involvement pattern raised suspicion, and the high IgG4 level with positive histology of the lacrimal gland confirmed the diagnosis of immunoglobulin G4-related orbitopathy. Rapid improvement was observed following oral methylprednisolone. CONCLUSIONS: IgG4-related orbitopathy may mimic Graves' orbitopathy. Euthyroid patients with no TSH receptor autoantibodies should be evaluated for immunoglobulin G4-related orbitopathy. Once IgG4-related orbitopathy is proven, other manifestations of IgG4-related disease have to be searched for; lifelong follow-up is warranted.
[Mh] MeSH terms primary: Antibodies, Anti-Idiotypic/immunology
Autoantibodies/immunology
Autoimmune Diseases/complications
Exophthalmos/etiology
Oculomotor Muscles/diagnostic imaging
[Mh] MeSH terms secundary: Adult
Autoimmune Diseases/diagnosis
Autoimmune Diseases/immunology
Diagnosis, Differential
Exophthalmos/diagnosis
Exophthalmos/immunology
Female
Graves Ophthalmopathy
Humans
Magnetic Resonance Imaging
Orbit
[Pt] Publication type:JOURNAL ARTICLE
[Nm] Name of substance:0 (Antibodies, Anti-Idiotypic); 0 (Autoantibodies); 0 (anti-IgA)
[Em] Entry month:1803
[Cu] Class update date: 180305
[Lr] Last revision date:180305
[Js] Journal subset:IM
[Da] Date of entry for processing:180114
[St] Status:MEDLINE
[do] DOI:10.1186/s12886-018-0672-y

  8 / 15998 MEDLINE  
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[PMID]: 29504670
[Au] Autor:Kumar M; Modi S; Rana P; Kumar P; Kanwar R; Sekhri T; D'souza M; Khushu S
[Ad] Address:NMR Research Centre, Institute of Nuclear Medicine and Allied Sciences (INMAS).
[Ti] Title:Alteration in intrinsic and extrinsic functional connectivity of resting state networks associated with subclinical hypothyroid.
[So] Source:J Neuroendocrinol;, 2018 Mar 05.
[Is] ISSN:1365-2826
[Cp] Country of publication:United States
[La] Language:eng
[Ab] Abstract:Subclinical hypothyroidism (SCH) is characterized by mild elevation of thyroid stimulating hormone (TSH) (range 5-10 µIU/ml) and normal free triiodothyronine (FT3) and free thyroxine (FT4). The cognitive function impairment is well known in thyroid disorders such as hypothyroidism and hyperthyroidism, but little is known about deficits in brain functions in SCH subjects. Also, whether hormone-replacement treatment is necessary or not in SCH subjects is still debatable. In order to have an insight into the cognition of SCH subjects, intrinsic and extrinsic functional connectivity (FC) of the resting state networks (RSNs) was studied. For resting state data analysis we used an unbiased, data-driven approach based on Independent Component Analysis (ICA) and dual-regression that can emphasize widespread changes in FC without restricting to a set of predefined seeds. 28 SCH subjects and 28 matched healthy controls (HC) participated in the study. RSN analysis showed significantly decreased intrinsic FC in somato-motor network (SMN) and right fronto-parietal attention network (RAN) and increased intrinsic FC in default mode network (DMN) in SCH subjects as compared to control subjects. The reduced intrinsic FC in the SMN and RAN suggests neuro-cognitive alterations in SCH subjects in the corresponding functions which were also evident from the deficit in the neuropsychological performance of the SCH subjects on behavioural tests such as digit span, delayed recall, visual retention, recognition, Bender Gestalt and Mini-Mental State Examination (MMSE). We also found a significant reduction in extrinsic network FC between DMN and RAN; SMN and posterior default mode network (PDMN); and increased extrinsic FC between SMN and anterior default mode network (ADMN) in SCH subjects as compared to controls. An altered extrinsic FC in SCH suggests functional reorganization in response to neurological disruption. The partial correlation analysis between intrinsic and extrinsic RSNs FC and neuropsychological performances as well as clinical indices give interesting insights into brain-behavior relationship in SCH subjects. This article is protected by copyright. All rights reserved.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180305
[Lr] Last revision date:180305
[St] Status:Publisher
[do] DOI:10.1111/jne.12587

  9 / 15998 MEDLINE  
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[PMID]: 29504633
[Au] Autor:Han Y; Mao LJ; Ge X; Huang K; Yan SQ; Ren LL; Hong SQ; Gao H; Sheng J; Xu YY; Pan WJ; Zhu P; Hao JH; Zhu DF; Tao FB
[Ad] Address:Department of Maternal, Child and Adolescent Health, School of Public Health, Anhui Medical University, Hefei, Anhui Province, China.
[Ti] Title:Thyroid autoantibodies in pregnancy are associated with hypertensive disorders of pregnancy: Ma'anshan Birth Cohort Study.
[So] Source:Clin Endocrinol (Oxf);, 2018 Mar 05.
[Is] ISSN:1365-2265
[Cp] Country of publication:England
[La] Language:eng
[Ab] Abstract:OBJECTIVE: Hypertensive disorders of pregnancy (HDP) have been associated with adverse health outcomes for both mothers and children. Previous studies examining associations of maternal thyroid autoantibodies with HDP indicate conflicting results. The objective of this study was to examine associations of maternal thyroid autoantibodies positivity in the first and the second trimesters with the risk of HDP. DESIGN, PARTICIPANTS AND MEASUREMENTS: In the Ma'anshan Birth Cohort study, a population-based prospective study in China, a total of 3474 pregnant women were enrolled between May 2013 and September 2014. Thyroid autoantibodies, including anti-thyroperoxidase autoantibody (TPOAb) and anti-thyroglobulin autoantibody (TgAb), as well as thyroid function tests were measured in both the first and the second trimesters in 2893 pregnant women. Multivariate logistic regression analyses were conducted to calculate the odds ratio (OR) and 95% confidence interval (CI) for the associations between thyroid autoantibodies and HDP. RESULTS: Multivariate logistic regression analyses showed that TPOAb positivity in the first trimester was associated with a 1.80 (95% CI=1.17-2.78) increased odds of HDP after adjustment for confounders, which was mainly due to an increased risk of gestational hypertension (OR=1.93, 95% CI=1.17-3.18). In addition, TgAb positivity in the first trimester was associated with a higher risk of HDP (OR=1.78, 95% CI=1.16-2.73) after adjustment for confounders, which was mainly due to an increased risk of gestational hypertension (OR=1.89, 95% CI=1.15-3.11). These associations were also seen among euthyroid women. Women with positive TPOAb in the second trimester seemed to have a higher risk of gestational hypertension (OR=1.87, 95% CI=1.02-3.43) after adjustment for confounders. However, among euthyroid women, TPOAb positivity in the second trimester was not associated with HDP. The TgAb status in the second trimester was not associated with HDP. CONCLUSIONS: Our results show that TPOAb positivity and TgAb positivity in the first trimester are associated with an increased risk of HDP. These data demonstrate that these associations are even seen among euthyroid women. This article is protected by copyright. All rights reserved.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1803
[Cu] Class update date: 180305
[Lr] Last revision date:180305
[St] Status:Publisher
[do] DOI:10.1111/cen.13590

  10 / 15998 MEDLINE  
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[PMID]: 29409959
[Au] Autor:Colter BT; Garber HF; Fleming SM; Fowler JP; Harding GD; Hooven MK; Howes AA; Infante SK; Lang AL; MacDougall MC; Stegman M; Taylor KR; Curran CP
[Ad] Address:Department of Biological Sciences, Northern Kentucky University, Highland Heights, KY, 41076, USA.
[Ti] Title:Ahr and Cyp1a2 genotypes both affect susceptibility to motor deficits following gestational and lactational exposure to polychlorinated biphenyls.
[So] Source:Neurotoxicology;65:125-134, 2018 Feb 15.
[Is] ISSN:1872-9711
[Cp] Country of publication:Netherlands
[La] Language:eng
[Ab] Abstract:Polychlorinated biphenyls (PCBs) are persistent organic pollutants known to cause adverse health effects and linked to neurological deficits in both human and animal studies. Children born to exposed mothers are at highest risk of learning and memory and motor deficits. We developed a mouse model that mimics human variation in the aryl hydrocarbon receptor and cytochrome P450 1A2 (CYP1A2) to determine if genetic variation increases susceptibility to developmental PCB exposure. In our previous studies, we found that high-affinity Ahr Cyp1a2(-/-) and poor-affinity Ahr Cyp1a2(-/-) knockout mice were most susceptible to learning and memory deficits following developmental PCB exposure compared with Ahr Cyp1a2(+/+) wild type mice (C57BL/6J strain). Our follow-up studies focused on motor deficits, because human studies have identified PCBs as a potential risk factor for Parkinson's disease. Dams were treated with an environmentally relevant PCB mixture at gestational day 10 and postnatal day 5. We used a motor battery that included tests of nigrostriatal function as well as cerebellar function, because PCBs deplete thyroid hormone, which is essential to normal cerebellar development. There was a significant effect of PCB treatment in the rotarod test with impaired performance in all three genotypes, but decreased motor learning as well in the two Cyp1a2(-/-) knockout lines. Interestingly, we found a main effect of genotype with corn oil-treated control Cyp1a2(-/-) mice performing significantly worse than Cyp1a2(+/+) wild type mice. In contrast, we found that PCB-treated high-affinity Ahr mice were most susceptible to disruption of nigrostriatal function with the greatest deficits in Ahr Cyp1a2(-/-) mice. We conclude that differences in AHR affinity combined with the absence of CYP1A2 protein affect susceptibility to motor deficits following developmental PCB exposure.
[Pt] Publication type:JOURNAL ARTICLE
[Em] Entry month:1802
[Cu] Class update date: 180304
[Lr] Last revision date:180304
[St] Status:Publisher


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