Database : MEDLINE Search on : ureteral and obstruction [Words] References found : 16062 [refine] Displaying: 1 .. 10   in format [Detailed]

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[PMID]: 29425932 [Au] Autor: Noh MR; Woo CH; Park MJ; In Kim J; Park KM [Ad] Address: Department of Anatomy and BK21 Plus, School of Medicine, Kyungpook National University, 680 Gukchaebosang-ro, Junggu, Daegu 41944, Republic of Korea. [Ti] Title: Ablation of C/EBP homologous protein attenuates renal fibrosis after ureteral obstruction by reducing autophagy and microtubule disruption. [So] Source: Biochim Biophys Acta;1864(5 Pt A):1634-1641, 2018 Feb 07. [Is] ISSN: 0006-3002 [Cp] Country of publication: Netherlands [La] Language: eng [Ab] Abstract: Fibrosis is an undesirable consequence of injury and a critical problem in many diseases. Recent studies have demonstrated an association of C/EBP homologous protein (CHOP) with fibrosis. We investigated the mechanism of CHOP in kidney fibrosis progression after unilateral ureteral obstruction (UUO) using Chop gene-deleted (Chop ) mice and their wild-type littermates (Chop ). UUO-induced kidney fibrosis was reduced in the Chop than Chop mice. After UUO, CHOP expression was detected in the cytosol and nucleus of distal tubule cells and collecting duct cells of the kidney. UUO formed the autophagosome and increased the expression of autophagy proteins, Beclin-1, LC3-I and II, and p62 in the kidneys. These UUO-induced changes were significantly reduced in Chop mice. Furthermore, Chop gene deletion attenuated mitochondrial fragmentation with lower expression of Fis-1, a mitochondrial fission protein, but higher expression of Opa-1, a mitochondrial fusion protein, than that seen in the wild-type mice. UUO disrupted the microtubule, which is involved in autophagosome formation, and this disruption was milder in the Chop than Chop mouse kidney, with less reduction of histone deacetylase 6 and α­tubulin acetyl transferase, which acetylates tubulin, a component of the microtubule. After UUO, apoptosis, a consequence of autophagy and mitochondrial damage, was reduced in the Chop mouse kidney cells than in Chop mice. Thus, the ablation of Chop attenuates renal fibrosis, accompanied by reduced autophagy, mitochondrial fragmentation, microtubule disruption, and apoptosis. Overall, these results suggest that CHOP plays a critical role in the progression of kidney fibrosis, likely through regulation of autophagy and apoptosis. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1802 [Cu] Class update date: 180310 [Lr] Last revision date: 180310 [St] Status: Publisher

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[PMID]: 29522906 [Au] Autor: Lin S; Lian D; Liu W; Haig A; Lobb I; Hijazi A; Razvi H; Burton J; Whiteman M; Sener A [Ad] Address: Department of Microbiology and Immunology, Western University, London, Ontario, Canada; Matthew Mailing Center for Translational Transplant Studies, London Health Sciences Center, London, Ontario, Canada. [Ti] Title: Daily therapy with a slow-releasing H S donor GYY4137 enables early functional recovery and ameliorates renal injury associated with urinary obstruction. [So] Source: Nitric Oxide;, 2018 Mar 06. [Is] ISSN: 1089-8611 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: OBJECTIVES: To assess the effects of slow-releasing H S donor GYY4137 on post-obstructive renal function and injury following unilateral ureteral obstruction (UUO) by using the UUO and reimplantation (UUO-R) model in rats and to elucidate potential mechanisms by using an in vitro model of epithelial-mesenchymal transition (EMT). METHODS: Male Lewis rats underwent UUO at the left ureterovesical junction. From post-obstructive day (POD) 1-13, rats received daily intraperitoneal (IP) injection of phosphate buffered saline (PBS, 1 mL) or GYY4137 (200 µmol/kg/day in 1 mL PBS, IP). On POD 14, the ureter was reimplanted back into the bladder, followed by a right nephrectomy. Urine and serum samples were collected to monitor renal function. On POD 30, the left kidney was removed and tissue sections were stained with H&E, TUNEL, CD68, CD206, myeloperoxidase, and Masson's trichrome to determine cortical thickness, apoptosis, inflammation, and fibrosis. In our in vitro model of EMT, NRK52E cells were treated with 10 ng/mL TGF-ß1, 10 µM GYY4137 and/or 50 µM GYY4137. Western blot analysis was performed to determine the expression of E-cadherin, vimentin, Smad7 and TGF-ß1 receptor II (TßRII). RESULTS: GYY4137 led to a moderate decrease in post-obstructive serum creatinine, cystatin C and FENa. We also observed a trend towards a decrease in post-obstructive proteinuria following GYY4137 treatment. Histologically, we observed a significant decrease in apoptosis, inflammation, and fibrosis. Furthermore, our in vitro studies demonstrate that in the presence of TGF-ß1, GYY4137 significantly decreases vimentin and TßRII and significantly increases E-cadherin and Smad7. CONCLUSIONS: H S may help to accelerate the recovery of renal function post-obstruction and attenuates renal injury associated with UUO. It is possible that H S mitigates fibrosis by regulating the TGF-ß1-mediated EMT pathway. Taken together, our data suggest that H S may be a potential novel therapy for improving renal function and limiting renal injury associated with obstructive uropathy. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1803 [Cu] Class update date: 180309 [Lr] Last revision date: 180309 [St] Status: Publisher

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[PMID]: 27771423 [Au] Autor: Chrzan R; Panek W; Kuijper CF; Dik P; Klijn AJ; de Mooij KL; de Jong TP [Ad] Address: Department of Pediatric Urology, University Children's Hospital AMC/EKZ, Amsterdam, The Netherlands; Department of Pediatric Urology, UMC/WKZ, Utrecht, The Netherlands. Electronic address: r.chrzan@amc.nl. [Ti] Title: Short-term Complications After Pyeloplasty in Children With Lower Urinary Tract Anomalies. [So] Source: Urology;100:198-202, 2017 Feb. [Is] ISSN: 1527-9995 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: OBJECTIVE: To investigate whether children with lower urinary tract (LUT) anomalies are at greater risk for postoperative complications after laparoscopic pyeloplasty stented with a double-J catheter (JJC). MATERIALS AND METHODS: Prospectively collected data of laparoscopic pyeloplasty (LP) performed between 2006 and 2015 were analyzed. Inclusion criteria are (1) toilet-trained child and (2) unilateral dismembered pyeloplasty stented with a JJC done by the same surgeon. Our pyeloplasty protocol includes cystoscopy and retrograde pyelography. JJC is left in for 3weeks. Asymptomatic patients with infravesical LUT anomalies (a-LUTA) and those with history of LUT symptoms (LUTS) were identified. Any short-term complication was classified according to Clavien-Dindo. Fisher's exact test was used for statistical analysis. RESULTS: Fifty-four children (mean 9.8 years) were included. Ten of 54 patients had LUTS. In 4 of those 10, anatomical infravesical anomaly was found during cystoscopy. Accidental urethral anomaly was found in 11 patients (a-LUTA). The control group (CG) consisted of 33 patients. Postoperative hospital stay ranged from 1 to 8 days (mean 2 days). Overall complication rate was 8 of 54 (14%). Grade 1 complications occurred in 3 patients in the CG. Five patients had grade 3 complications (2 needed replacement of bladder catheter, and 3 had diversion of the upper tract). Those problems occurred in 1 of 10 patients with LUTS and 3 of 11 patients with a-LUTA compared to 1 of 33 in the CG. This difference was statistically significant (P < .05). CONCLUSION: Careful history should be taken in toilet-trained children before pyeloplasty. If any infravesical abnormality is discovered, internal diversion should probably be avoided. Special attention must be paid to bladder function in the postoperative period. [Mh] MeSH terms primary: Kidney Pelvis/surgery Laparoscopy/adverse effects Postoperative Complications/epidemiology Reconstructive Surgical Procedures/adverse effects Ureteral Obstruction/surgery Urogenital Abnormalities/surgery [Mh] MeSH terms secundary: Asymptomatic Diseases Child Cystoscopy Female Humans Lower Urinary Tract Symptoms/etiology Lower Urinary Tract Symptoms/surgery Male Stents Ureteral Obstruction/etiology Urography [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1803 [Cu] Class update date: 180309 [Lr] Last revision date: 180309 [Js] Journal subset: IM [Da] Date of entry for processing: 161025 [St] Status: MEDLINE

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[PMID]: 29521269 [Au] Autor: Yuksel OH; Yildirim C; Urkmez A; Ozbay N; Uruc F; Sahin A; Akan S; Verit A [Ad] Address: Department of Urology. Fatih Sultan Mehmet Research & Training Hospital. Istanbul. Turkey. [Ti] Title: Efecto de darbepoyetina alfa sobre la fibrosis renal en ratas con obstruccion ureteral unilateral aguda. The effect of darbepoetin alfa on renal fibrosis in rats with acute unilateral ureteral obstruction. [So] Source: Arch Esp Urol;71(2):212-221, 2018 Mar. [Is] ISSN: 0004-0614 [Cp] Country of publication: Spain [La] Language: eng [Ab] Abstract: OBJECTIVES: The most important treatment strategy for obstructive nephropathy is to protect renal tissue from the deleterious effects of fibrosis. Therefore, we sought to investigate the renoprotective effects of darbepoetin alfa on unilateral ureteral obstructions. METHODS: We used 12 female and 12 male 3-monthold Wistar rats weighing between 250 and 350 g. The rats were divided equally into sham, darbepoetin and control groups. With the exception of the sham group, left unilateral obstructions were applied to all of the rats. The darbepoetin group received perioperative darbepoetin alfa at a dose of 10 mg/kg. The rats were sacrificed on postoperative day 7, and 3-cc blood samples and bilateral renal specimens were collected from each rat. RESULTS: Renal ectasia was observed significantly less frequently in the darbepoetin group than the obstruction group (p<0.001). Additionally, the uptake rates of cortical TNF and medullary SMA in the darbepoetin group were comparable to those in the sham group but lower than those in the ureteral obstruction group (p<0.001 and p<0.008, respectively). When biomarkers of renal injury, including cystatin-C, malondialdehyde, and B2 microglobulin, were evaluated in combination, B2 microglobulin was found at higher levels in the ureteral obstruction group (p<0.004). CONCLUSION: As we know pelvicalyceal ectasia reflects intrapelvic pressure into renal tubular system via renal reflux. Therefore pelvicalyceal ectasia can be used as an indicator of renal tubular pressure. Although as a limitation of our study, renal tubular pressure was not quantitatively evaluated, parallelism between levels of renal ectasia detected in the rats of the sham, and DPO groups can predict that this drug (darbepoetin-a) can decrease renal tubular pressure in acute ureteral obstruction. Moreover, B2 microglobulin levels in the sham, and DPO groups differed from those of ureteral obstruction group, which suggested that DPO does not impair renal perfusion in addition to its decreasing effects on renal tubular pressure. We think that in countries with higher incidence rates of stone disease similar to our country, DPO may be used among medical treatment alternatives, which aim to preserve renal reserve. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1803 [Cu] Class update date: 180309 [Lr] Last revision date: 180309 [St] Status: In-Data-Review

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[PMID]: 29518138 [Au] Autor: Ghosh M; Thangada S; Dasgupta O; Khanna KM; Yamase HT; Kashgarian M; Hla T; Shapiro LH; Ferrer FA [Ad] Address: Center for Vascular Biology, University of Connecticut School of Medicine, Farmington, CT, United States of America. [Ti] Title: Cell-intrinsic sphingosine kinase 2 promotes macrophage polarization and renal inflammation in response to unilateral ureteral obstruction. [So] Source: PLoS One;13(3):e0194053, 2018. [Is] ISSN: 1932-6203 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Sphingosine Kinase-2 (Sphk2) is responsible for the production of the bioactive lipid Sphingosine-1 Phosphate, a key regulator of tissue repair. Here we address the in vivo significance of Sphingosine Kinase -2 in renal inflammation/fibrosis in response to unilateral ureteral obstruction using both genetic and pharmacological strategies. Obstructed kidneys of Sphk2-/- mice showed reduced renal damage and diminished levels of the renal injury markers TGFß1 and αSMA when compared to wild type controls. We found a consistently significant increase in anti-inflammatory (M2) macrophages in obstructed Sphk2-/- kidneys by flow cytometry and a decrease in mRNA levels of the inflammatory cytokines, MCP1, TNFα, CXCL1 and ILß1, suggesting an anti-inflammatory bias in the absence of Sphk2. Indeed, metabolic profiling showed that the pro-inflammatory glycolytic pathway is largely inactive in Sphk2-/- bone marrow-derived macrophages. Furthermore, treatment with the M2-promoting cytokines IL-4 or IL-13 demonstrated that macrophages lacking Sphk2 polarized more efficiently to the M2 phenotype than wild type cells. Bone marrow transplant studies indicated that expression of Sphk2-/- on either the hematopoietic or parenchymal cells did not fully rescue the pro-healing phenotype, confirming that both infiltrating M2-macrophages and the kidney microenvironment contribute to the damaging Sphk2 effects. Importantly, obstructed kidneys from mice treated with an Sphk2 inhibitor recapitulated findings in the genetic model. These results demonstrate that reducing Sphk2 activity by genetic or pharmacological manipulation markedly decreases inflammatory and fibrotic responses to obstruction, resulting in diminished renal injury and supporting Sphk2 as a novel driver of the pro-inflammatory macrophage phenotype. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1803 [Cu] Class update date: 180308 [Lr] Last revision date: 180308 [St] Status: In-Data-Review [do] DOI: 10.1371/journal.pone.0194053

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[PMID]: 29415885 [Au] Autor: Imamura M; Moon JS; Chung KP; Nakahira K; Muthukumar T; Shingarev R; Ryter SW; Choi AM; Choi ME [Ad] Address: Division of Pulmonary and Critical Care Medicine, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medical College, New York, New York, USA. [Ti] Title: RIPK3 promotes kidney fibrosis via AKT-dependent ATP citrate lyase. [So] Source: JCI Insight;3(3), 2018 Feb 08. [Is] ISSN: 2379-3708 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Renal fibrosis is a common pathogenic response to injury in chronic kidney disease (CKD). The receptor-interacting protein kinase-3 (RIPK3), a regulator of necroptosis, has been implicated in disease pathogenesis. In mice subjected to unilateral ureteral obstruction-induced (UUO-induced) or adenine diet-induced (AD-induced) renal fibrosis, models of progressive kidney fibrosis, we demonstrate increased kidney expression of RIPK3. Mice genetically deficient in RIPK3 displayed decreased kidney fibrosis and improved kidney function relative to WT mice when challenged with UUO or AD. In contrast, mice genetically deficient in mixed-lineage kinase domain-like protein (MLKL), a downstream RIPK3 target, were not protected from UUO-induced kidney fibrosis. We demonstrate a pathway by which RIPK3 promotes fibrogenesis through the AKT-dependent activation of ATP citrate lyase (ACL). Genetic or chemical inhibition of RIPK3 suppressed the phosphorylation of AKT and ACL in response to TGF-ß1 in fibroblasts. Inhibition of AKT or ACL suppressed TGF-ß1-dependent extracellular matrix production and myofibroblast differentiation in fibroblasts. Pharmacological inhibition of ACL suppressed UUO-induced kidney fibrosis. RIPK3 expression was highly regulated in human CKD kidney. In conclusion, we identify a pathway by which RIPK3 promotes kidney fibrosis independently of MLKL-dependent necroptosis as a promising therapeutic target in CKD. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1802 [Cu] Class update date: 180309 [Lr] Last revision date: 180309 [St] Status: Publisher

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[PMID]: 29391290 [Au] Autor: Thoreau A; Tran PL; Gabriele M; Flye Sainte Marie H; Boukerrou M [Ad] Address: Department of Gynecology and Obstetrics, University Hospital of South Reunion Island, BP 350, 97448 Saint-Pierre cedex, Reunion; Faculty of Medicine, University of Reunion, 97490 St-Denis, Reunion. [Ti] Title: Ureteral obstruction and ruptured kidney following ovarian hyperstimulation syndrome. [So] Source: J Gynecol Obstet Hum Reprod;, 2018 Jan 31. [Is] ISSN: 2468-7847 [Cp] Country of publication: France [La] Language: eng [Ab] Abstract: In vitro fertilization (IVF) is nowadays a reliable and common method for couple who need medically assisted procreation. Complications are rare. We report in this paper, the case of a woman with severe endometriosis who developed ureteral obstruction complicated by a renal rupture of the fornix due to ovarian hyperstimulation during an IVF attempt. The condition was diagnosed by CT scan and resolved with insertion of double-J catheter in the left ureter. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1802 [Cu] Class update date: 180309 [Lr] Last revision date: 180309 [St] Status: Publisher

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[PMID]: 29504770 [Au] Autor: Chen HH; Lee TT; Chen A; Hwu Y; Petibois C [Ad] Address: Academia Sinica, Institute of Physics , 128 Sec. 2, Academia Road, Nankang , Taipei 11529 , Taiwan. [Ti] Title: 3D Digital Pathology for a Chemical-Functional Analysis of Glomeruli in Health and Pathology. [So] Source: Anal Chem;, 2018 Mar 08. [Is] ISSN: 1520-6882 [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: Determining the filtration function and biochemical status of kidney at the single glomerulus level remains hardly accessible, even from biopsies. Here, we provide evidence that IR spectro-microscopy is a suitable method to account for the filtration capacity of individual glomeruli along with related physio-pathological condition. A ∼4 µm voxel resolution 3D IR image reconstruction is built from consecutive tissue sections, thus, providing a 3D IR spectrum matrix of an individual glomerulus. The filtration capacity of glomeruli was quantitatively determined after BaSO perfusion, and additional chemical data could be used to determined oxidative stress effects and fibrosis, thus, combining functional and biochemical information from the same 3D IR spectrum matrix. This analytical approach was applied on mice with unilateral ureteral obstruction (UUO) inducing chronic kidney disease. Compared to the healthy condition, UUO induced a significant drop in glomeruli filtration capacity (-17 ± 8% at day 4 and -48 ± 14% at day 14) and volume (36 ± 10% at day 4 and 67 ± 13% at day 14), along a significant increase of oxidative stress (+61 ± 19% at day 4 and +84 ± 17% at day 14) and a change in the lipid-to-protein ratio (-8.2 ± 3.6% at day 4 and -18.1 ± 5.9% at day 14). Therefore, IR spectro-microscopy might be developed as a new 3D pathology resource for analyzing functional and biochemical parameters of glomeruli. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1803 [Cu] Class update date: 180308 [Lr] Last revision date: 180308 [St] Status: Publisher [do] DOI: 10.1021/acs.analchem.7b04265

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[PMID]: 29436600 [Au] Autor: Qi FH; Cai PP; Liu X; Si GM [Ad] Address: Department of Traditional Chinese Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong 250021, P.R. China. [Ti] Title: Adenovirus-mediated P311 ameliorates renal fibrosis through inhibition of epithelial-mesenchymal transition via TGF-ß1-Smad-ILK pathway in unilateral ureteral obstruction rats. [So] Source: Int J Mol Med;41(5):3015-3023, 2018 May. [Is] ISSN: 1791-244X [Cp] Country of publication: Greece [La] Language: eng [Ab] Abstract: Epithelial-mesenchymal transition (EMT) is a critical step and key factor during renal fibrosis. Preventing renal tubular EMT is important for delaying the progression of chronic kidney disease (CKD). P311, a highly conserved 8-kDa intracellular protein, has been indicated as an important factor in myofibroblast transformation and in the progression of fibrosis. However, the related studies on P311 on renal fibrosis are limited and the mechanisms of P311 in the progression of renal tubulointerstitial fibrosis remain largely unknown. In the present study, we examined the effect of P311 on transforming growth factor-ß1 (TGF-ß1)-mediated EMT in a rat model of unilateral ureteral occlusion (UUO) renal fibrosis. The recombinant adenovirus p311 (also called Ad-P311) was constructed and transferred it into UUO rats, the preventive effect and possible mechanism of P311 on TGF-ß1-mediated EMT were explored. The UUO model was established successfully and Ad-P311 was administered into UUO rats each week for 4 weeks, then the serum levels of Cr, blood urea nitrogen (BUN) and albumin (ALB) were evaluated. H&E staining and Masson staining were performed to observe the pathological changes of kidneys. Immunohistochemical staining and western blot analysis were used to examine the EMT markers [E-cadherin and α-smooth muscle actin (α-SMA)], and signal transducers (p-Smad2/3 and Smad7). Integrin linked kinase (ILK) as a keyintracellular mediator that controls TGF-ß1-mediated-EMT was also assayed by western blot analysis. The results showed that P311 could alleviate renal tubular damage and interstitial fibrosis improving Cr, BUN and ALB serum levels in UUO kidneys. Furthermore, P311 attenuated TGF-ß1-mediated EMT through Smad-ILK signaling pathway with an increase in α-SMA, pSmad2/3 and ILK expression, and a decrease in E-cadherin and Smad7 expression in UUO kidneys. In conclusion, P311 may be involved in the pathogenesis of renal fibrosis by blocking TGF-ß1-mediated EMT via TGF-ß1-Smad-ILK pathway in UUO kidneys. P311 may be a novel target for the control of renal fibrosis and the progression of CKD. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1802 [Cu] Class update date: 180307 [Lr] Last revision date: 180307 [St] Status: In-Process [do] DOI: 10.3892/ijmm.2018.3485

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[PMID]: 29234836 [Au] Autor: Carrafiello G; Coppola A; De Marchi G; Fontana F; Piacentino F; Petrillo M; Taborelli A; Angileri SA; Xhepa G; Macchione N; Bacuzzi A; Marconi A; Ierardi AM [Ad] Address: Diagnostic and Interventional Radiology Department, San Paolo Hospital, University of Milan, Milan, Italy. gcarraf@gmail.com. [Ti] Title: Trans-Urethral Ureteral Stent Replacement Technique (TRUST): 10-Year Experience in 1168 Patients. [So] Source: Cardiovasc Intervent Radiol;41(4):610-617, 2018 Apr. [Is] ISSN: 1432-086X [Cp] Country of publication: United States [La] Language: eng [Ab] Abstract: OBJECTIVES: To affirm technical success, clinical success and safety of fluoroscopically guided transurethral replacement of double-J (DJ) ureteral stents. METHODS: From January 2005 to December 2015, in a follow-up period ranging from 9 to 73 months, we replaced 6167 DJ ureteral stents in 3221 procedures in 1168 patients. All the procedures were performed in the angiography suite under fluoroscopic control. RESULTS: Technical success was achieved in 97.5% of the procedures. In eighty procedures, cystoscopic approach was necessary; time from previous procedure and side were significantly associated with technical success. Clinical success was reached in 95.7% of the procedures and was significantly lower in urological and gynaecological tumours (when compared to fibrosis and other causes) and in bilateral stents. No major complications were reported. In 90 cases, self-limiting transient minor haematuria occurred and in 160 procedures urinary tract infection responding to antibiotics were registered. Overall procedure time was 27 min. Mean fluoroscopic time was 6 min and 45 s. Mean radiation dose of the procedure was 38.40 Gy cm . CONCLUSIONS: In patients that need routine replacement of DJ ureteral stent, transurethral fluoroscopically guided method may be the first choice; only in few cases of technical failure, cystoscopy may be considered. [Pt] Publication type: JOURNAL ARTICLE [Em] Entry month: 1712 [Cu] Class update date: 180306 [Lr] Last revision date: 180306 [St] Status: In-Process [do] DOI: 10.1007/s00270-017-1854-3

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