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  1 / 8792 MEDLINE  
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[PMID]:28886051
[Au] Autor:Baek IJ; Moss DS; Lustig AJ
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Tulane University Medical Center, New Orleans, Louisiana, United States of America.
[Ti] Título:The mre11 A470 alleles influence the hereditability and the segregation of telosomes in Saccharomyces cerevisiae.
[So] Source:PLoS One;12(9):e0183549, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Telomeres, the nucleoprotein complexes at the termini of linear chromosomes, are essential for the processes of end replication, end protection, and chromatin segregation. The Mre11 complex is involved in multiple cellular roles in DNA repair and structure in the regulation and function of telomere size homeostasis. In this study, we characterize yeast telomere chromatin structure, phenotypic heritability, and chromatin segregation in both wild-type [MRE11] and A470 motif alleles. MRE11 strains confer a telomere size of 300 base pairs of G+T irregular simple sequence repeats. This DNA and a portion of subtelomeric DNA is embedded in a telosome: a MNase-resistant non-nucleosomal particle. Chromatin immunoprecipitation shows a three to four-fold lower occupancy of Mre11A470T proteins than wild-type proteins in telosomes. Telosomes containing the Mre11A470T protein confer a greater resistance to MNase digestion than wild-type telosomes. The integration of a wild-type MRE11 allele into an ectopic locus in the genome of an mre11A470T mutant and the introduction of an mre11A470T allele at an ectopic site in a wild-type strain lead to unexpectedly differing results. In each case, the replicated sister chromatids inherit telosomes containing only the protein encoded by the genomic mre11 locus, even in the presence of protein encoded by the opposing ectopic allele. We hypothesize that the telosome segregates by a conservative mechanism. These data support a mechanism for the linkage between sister chromatid replication and maintenance of either identical mutant or identical wild-type telosomes after replication of sister chromatids. These data suggest the presence of an active mechanism for chromatin segregation in yeast.
[Mh] Termos MeSH primário: Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Telômero/genética
Telômero/metabolismo
[Mh] Termos MeSH secundário: Alelos
Cromátides/genética
Cromátides/metabolismo
Imunoprecipitação da Cromatina
Mutação/genética
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183549


  2 / 8792 MEDLINE  
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[PMID]:28817615
[Au] Autor:Luaces JP; Rossi LF; Chirino MG; Browne M; Merani MS; Mudry MD
[Ad] Endereço:Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina.
[Ti] Título:Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies.
[So] Source:PLoS One;12(8):e0182911, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural areas where soybean is the predominant crop. In such areas the pesticide Roundup Full II® (RU) is widely applied. The genotoxic effect of its active ingredient glyphosate (RU is 66.2% glyphosate) on the peripheral blood lymphocytes of C. villosus was tested over a range of concentrations (280, 420, 560, 1120 µmol/L). Culture medium without glyphosate served as negative control, while medium containing mitomycin C served as positive control. Genetic damage was characterized in terms of the percentage of cells with chromosome aberrations (CA), the mean number of sister chromatid exchanges (SCE) per cell, and the modification of cell proliferation kinetics via the calculation of the replication index. Significant increases (p < 0.0001) were seen in the CA frequency and the mean number of SCEs per cell compared to negative controls at all the RU concentrations tested. Chromatid breaks, the only form of CA observed, under the 560 µmol/L RU conditions and in presence of mitomycin C were four to five times more common than at lower concentrations, while no viable cells were seen in the 1120 µmol/L treatment. The mean number of SCEs per cell was significantly higher under the 280 µmol/L RU conditions than the 420 or 560 µmol/L RU conditions; cells cultivated in the presence of MMC also showed significantly more SCEs. All the RU concentrations tested (except in the 1120 µmol/L RU treatment [no viable cells]) induced a significant reduction in the replication index (p < 0.0001). The present results confirm the genotoxic effects of RU on C. villosus lymphocytes in vitro, strongly suggesting that exposure to RU could induce DNA damage in C. villosus wildlife.
[Mh] Termos MeSH primário: Dano ao DNA
Glicina/análogos & derivados
Linfócitos/efeitos de drogas
Praguicidas/toxicidade
Xenartros/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Quebra Cromossômica
Replicação do DNA
Feminino
Glicina/efeitos adversos
Glicina/toxicidade
Masculino
Praguicidas/efeitos adversos
Troca de Cromátide Irmã
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticides); 4632WW1X5A (glyphosate); TE7660XO1C (Glycine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182911


  3 / 8792 MEDLINE  
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[PMID]:28911102
[Au] Autor:Nath S; Somyajit K; Mishra A; Scully R; Nagaraju G
[Ad] Endereço:Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
[Ti] Título:FANCJ helicase controls the balance between short- and long-tract gene conversions between sister chromatids.
[So] Source:Nucleic Acids Res;45(15):8886-8900, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The FANCJ DNA helicase is linked to hereditary breast and ovarian cancers as well as bone marrow failure disorder Fanconi anemia (FA). Although FANCJ has been implicated in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR), the molecular mechanism underlying the tumor suppressor functions of FANCJ remains obscure. Here, we demonstrate that FANCJ deficient human and hamster cells exhibit reduction in the overall gene conversions in response to a site-specific chromosomal DSB induced by I-SceI endonuclease. Strikingly, the gene conversion events were biased in favour of long-tract gene conversions in FANCJ depleted cells. The fine regulation of short- (STGC) and long-tract gene conversions (LTGC) by FANCJ was dependent on its interaction with BRCA1 tumor suppressor. Notably, helicase activity of FANCJ was essential for controlling the overall HR and in terminating the extended repair synthesis during sister chromatid recombination (SCR). Moreover, cells expressing FANCJ pathological mutants exhibited defective SCR with an increased frequency of LTGC. These data unravel the novel function of FANCJ helicase in regulating SCR and SCR associated gene amplification/duplications and imply that these functions of FANCJ are crucial for the genome maintenance and tumor suppression.
[Mh] Termos MeSH primário: Proteína BRCA1/genética
Fatores de Transcrição de Zíper de Leucina Básica/genética
Cromátides/química
DNA/genética
Proteínas de Grupos de Complementação da Anemia de Fanconi/genética
Reparo de DNA por Recombinação
[Mh] Termos MeSH secundário: Animais
Proteína BRCA1/metabolismo
Fatores de Transcrição de Zíper de Leucina Básica/metabolismo
Células CHO
Linhagem Celular Tumoral
Cromátides/metabolismo
Cricetulus
DNA/metabolismo
Quebras de DNA de Cadeia Dupla
Desoxirribonucleases de Sítio Específico do Tipo II/farmacologia
Proteínas de Grupos de Complementação da Anemia de Fanconi/metabolismo
Pontos de Checagem da Fase G2 do Ciclo Celular
Regulação da Expressão Gênica
Recombinação Homóloga/efeitos de drogas
Humanos
Mutação
Osteoblastos/citologia
Osteoblastos/efeitos de drogas
Osteoblastos/metabolismo
Ligação Proteica
Proteínas de Saccharomyces cerevisiae/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BACH1 protein, human); 0 (BRCA1 Protein); 0 (BRCA1 protein, human); 0 (Basic-Leucine Zipper Transcription Factors); 0 (Fanconi Anemia Complementation Group Proteins); 0 (Saccharomyces cerevisiae Proteins); 9007-49-2 (DNA); EC 3.1.21.- (SCEI protein, S cerevisiae); EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170915
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx586


  4 / 8792 MEDLINE  
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[PMID]:28797117
[Au] Autor:Singh G; Da Ines O; Gallego ME; White CI
[Ad] Endereço:Génétique, Reproduction et Dévelopement, UMR CNRS 6293 - INSERM U1103 - Université Cleront Auvergne Campus Universitaire des Cézeaux, Aubiere, France.
[Ti] Título:Analysis of the impact of the absence of RAD51 strand exchange activity in Arabidopsis meiosis.
[So] Source:PLoS One;12(8):e0183006, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ploidy of eukaryote gametes must be halved to avoid doubling of numbers of chromosomes with each generation and this is carried out by meiosis, a specialized cell division in which a single chromosomal replication phase is followed by two successive nuclear divisions. With some exceptions, programmed recombination ensures the proper pairing and distribution of homologous pairs of chromosomes in meiosis and recombination defects thus lead to sterility. Two highly related recombinases are required to catalyse the key strand-invasion step of meiotic recombination and it is the meiosis-specific DMC1 which is generally believed to catalyse the essential non-sister chromatid crossing-over, with RAD51 catalysing sister-chromatid and non-cross-over events. Recent work in yeast and plants has however shown that in the absence of RAD51 strand-exchange activity, DMC1 is able to repair all meiotic DNA breaks and surprisingly, that this does not appear to affect numbers of meiotic cross-overs. In this work we confirm and extend this conclusion. Given that more than 95% of meiotic homologous recombination in Arabidopsis does not result in inter-homologue crossovers, Arabidopsis is a particularly sensitive model for testing the relative importance of the two proteins-even minor effects on the non-crossover event population should produce detectable effects on crossing-over. Although the presence of RAD51 protein provides essential support for the action of DMC1, our results show no significant effect of the absence of RAD51 strand-exchange activity on meiotic crossing-over rates or patterns in different chromosomal regions or across the whole genome of Arabidopsis, strongly supporting the argument that DMC1 catalyses repair of all meiotic DNA breaks, not only non-sister cross-overs.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/citologia
Quebras de DNA
Rad51 Recombinase/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Cromátides/genética
Cromátides/metabolismo
Reparo do DNA
Recombinação Homóloga
Meiose
Rad51 Recombinase/genética
Recombinação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); EC 2.7.7.- (Rad51 Recombinase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183006


  5 / 8792 MEDLINE  
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[PMID]:28719910
[Au] Autor:Bustamante FO; Aliyeva-Schnorr L; Fuchs J; Beier S; Houben A
[Ad] Endereço:Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
[Ti] Título:Correlating the Genetic and Physical Map of Barley Chromosome 3H Revealed Limitations of the FISH-Based Mapping of Nearby Single-Copy Probes Caused by the Dynamic Structure of Metaphase Chromosomes.
[So] Source:Cytogenet Genome Res;152(2):90-96, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Genetic maps are based on the recombination frequency of molecular markers which often show different positions in comparison to the corresponding physical maps. To decipher the position and order of DNA sequences genetically mapped to terminal and interstitial regions of barley (Hordeum vulgare) chromosome 3H, fluorescence in situ hybridization (FISH) on mitotic metaphase chromosomes was performed with 16 genomic single-copy probes derived from fingerprinted BAC contigs. Long genetic distances at subterminal regions translated into short physical distances, confirming that recombination events occur more often at distal regions of chromosome 3H. Nonoverlapping FISH signals were frequently obtained for probes with a physical distance of at least 30-60 kb. Only 8% of the analyzed chromosomes showed a symmetric order of FISH signals on both sister chromatids. Due to the dynamic packing of metaphase chromatin, the order of 2 adjacent single-copy signals along the chromosome arms outside the (peri)centromeric region can only reliably be determined if the cytological distance is approximately 3%, corresponding to 21.6 Mb.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Cromossomos de Plantas/genética
Dosagem de Genes
Hordeum/genética
Hibridização in Situ Fluorescente/métodos
Metáfase/genética
Mapeamento Físico do Cromossomo/métodos
[Mh] Termos MeSH secundário: Pareamento de Bases/genética
Cromátides/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1159/000478631


  6 / 8792 MEDLINE  
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[PMID]:28781233
[Au] Autor:Krishnan S; Smits AH; Vermeulen M; Reinberg D
[Ad] Endereço:Howard Hughes Medical Institute, New York University Langone School of Medicine, New York, NY 10016, USA; Department of Biochemistry and Molecular Pharmacology, New York University Langone School of Medicine, New York, NY 10016, USA.
[Ti] Título:Phospho-H1 Decorates the Inter-chromatid Axis and Is Evicted along with Shugoshin by SET during Mitosis.
[So] Source:Mol Cell;67(4):579-593.e6, 2017 Aug 17.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Precise control of sister chromatid separation during mitosis is pivotal to maintaining genomic integrity. Yet, the regulatory mechanisms involved are not well understood. Remarkably, we discovered that linker histone H1 phosphorylated at S/T18 decorated the inter-chromatid axial DNA on mitotic chromosomes. Sister chromatid resolution during mitosis required the eviction of such H1S/T18ph by the chaperone SET, with this process being independent of and most likely downstream of arm-cohesin dissociation. SET also directed the disassembly of Shugoshins in a polo-like kinase 1-augmented manner, aiding centromere resolution. SET ablation compromised mitotic fidelity as evidenced by unresolved sister chromatids with marked accumulation of H1S/T18ph and centromeric Shugoshin. Thus, chaperone-assisted eviction of linker histones and Shugoshins is a fundamental step in mammalian mitotic progression. Our findings also elucidate the functional implications of the decades-old observation of mitotic linker histone phosphorylation, serving as a paradigm to explore the role of linker histones in bio-signaling processes.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
Cromátides/metabolismo
Chaperonas de Histonas/metabolismo
Histonas/metabolismo
Mitose
Proteínas Oncogênicas/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Ciclo Celular/genética
Cromátides/genética
Segregação de Cromossomos
Fibroblastos/metabolismo
Células HEK293
Chaperonas de Histonas/genética
Humanos
Camundongos
Proteínas Oncogênicas/genética
Fosforilação
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas Proto-Oncogênicas/genética
Proteínas Proto-Oncogênicas/metabolismo
Interferência de RNA
Epitélio Pigmentado da Retina/metabolismo
Transdução de Sinal
Fatores de Transcrição/genética
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Histone Chaperones); 0 (Histones); 0 (Oncogene Proteins); 0 (Proto-Oncogene Proteins); 0 (SET protein, human); 0 (SET protein, mouse); 0 (SGOL1 protein, human); 0 (Transcription Factors); 0 (shugoshin protein, mouse); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (polo-like kinase 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170807
[St] Status:MEDLINE


  7 / 8792 MEDLINE  
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[PMID]:28263986
[Au] Autor:Akpinar M; Lesche M; Fanourgakis G; Fu J; Anastassiadis K; Dahl A; Jessberger R
[Ad] Endereço:Institute of Physiological Chemistry, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.
[Ti] Título:TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes.
[So] Source:PLoS Genet;13(3):e1006660, 2017 03.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility. Here we show that in meiotic prophase I spermatocytes TDRD6 interacts with the key protein arginine methyl transferase PRMT5, which supports splicing. TDRD6 also associates with spliceosomal core protein SmB in the absence of RNA and in an arginine methylation dependent manner. In Tdrd6-/- diplotene spermatocytes PRMT5 association with SmB and arginine dimethylation of SmB are much reduced. TDRD6 deficiency impairs the assembly of spliceosomes, which feature 3.5-fold increased levels of U5 snRNPs. In the nucleus, these deficiencies in spliceosome maturation correlate with decreased numbers of SMN-positive bodies and Cajal bodies involved in nuclear snRNP maturation. Transcriptome analysis of TDRD6-deficient diplotene spermatocytes revealed high numbers of splicing defects such as aberrant usage of intron and exons as well as aberrant representation of splice junctions. Together, this study demonstrates a novel function of TDRD6 in spliceosome maturation and mRNA splicing in prophase I spermatocytes.
[Mh] Termos MeSH primário: Proteína-Arginina N-Metiltransferases/metabolismo
Ribonucleoproteína Nuclear Pequena U5/metabolismo
Ribonucleoproteínas/genética
Ribonucleoproteínas/fisiologia
Espermatócitos/metabolismo
Spliceossomos/metabolismo
[Mh] Termos MeSH secundário: Animais
Arginina/química
Cromátides/química
Corpos Enovelados/metabolismo
Metilação de DNA
Desoxiadenosinas/química
Éxons
Ácidos Graxos Insaturados/química
Íntrons
Masculino
Metilação
Camundongos
Camundongos Transgênicos
Microscopia de Fluorescência
Domínios Proteicos
Processamento de RNA
RNA Mensageiro/metabolismo
Espermatócitos/citologia
Tionucleosídeos/química
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Deoxyadenosines); 0 (Fatty Acids, Unsaturated); 0 (RNA, Messenger); 0 (Ribonucleoprotein, U5 Small Nuclear); 0 (Ribonucleoproteins); 0 (TDRD6 protein, mouse); 0 (Thionucleosides); 634Z2VK3UQ (5'-methylthioadenosine); 94ZLA3W45F (Arginine); EC 2.1.1.319 (Prmt5 protein, mouse); EC 2.1.1.319 (Protein-Arginine N-Methyltransferases); Y031I2N1EO (leptomycin B)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006660


  8 / 8792 MEDLINE  
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[PMID]:28188342
[Au] Autor:Liu J; Zhang C
[Ad] Endereço:Department of Biological Sciences, California State Polytechnic University, Pomona, CA, 91768, USA. junjunliu@cpp.edu.
[Ti] Título:The equilibrium of ubiquitination and deubiquitination at PLK1 regulates sister chromatid separation.
[So] Source:Cell Mol Life Sci;74(12):2127-2134, 2017 Jun.
[Is] ISSN:1420-9071
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:PLK1 regulates almost every aspect of mitotic events, including mitotic entry, spindle assembly, chromosome alignment, sister chromatid segregation, metaphase-anaphase transition, cytokinesis, etc. In regulating the chromosome alignment and sister chromatid segregation, PLK1 has to be localized to and removed from kinetochores at the right times, and the underlying mechanism that regulates PLK1 both spatially and temporally only became clearer recently. It has been found that deubiquitination and ubiquitination of PLK1 are responsible for its localization to and dissociation from the kinetochores, respectively. The equilibrium of this ubiquitination and deubiquitination plays an important role in regulating proper chromosome alignment and timely sister chromatid segregation. Here, we summarize and discuss the recent findings in investigating the spatial and temporal regulation of PLK1 during chromosome alignment and sister chromatid segregation.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
Cromátides/metabolismo
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas Proto-Oncogênicas/metabolismo
Ubiquitinação
[Mh] Termos MeSH secundário: Animais
Ciclo Celular
Humanos
Cinetocoros/metabolismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Proto-Oncogene Proteins); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (polo-like kinase 1)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1007/s00018-017-2457-5


  9 / 8792 MEDLINE  
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[PMID]:28360182
[Au] Autor:Wu X; Inoue A; Suzuki T; Zhang Y
[Ad] Endereço:Howard Hughes Medical Institute, Boston, Massachusetts 02115, USA.
[Ti] Título:Simultaneous mapping of active DNA demethylation and sister chromatid exchange in single cells.
[So] Source:Genes Dev;31(5):511-523, 2017 Mar 01.
[Is] ISSN:1549-5477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To understand mammalian active DNA demethylation, various methods have been developed to map the genomic distribution of the demethylation intermediates 5-formylcysotine (5fC) and 5-carboxylcytosine (5caC). However, the majority of these methods requires a large number of cells to begin with. In this study, we describe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) and single-cell MAB-seq (scMAB-seq), capable of profiling 5fC and 5caC at genome scale using ∼100 cells and single cells, respectively. liMAB-seq analysis of preimplantation embryos reveals the oxidation of 5mC to 5fC/5caC and the positive correlation between chromatin accessibility and processivity of ten-eleven translocation (TET) enzymes. scMAB-seq captures the cell-to-cell heterogeneity of 5fC and 5caC and reveals the strand-biased distribution of 5fC and 5caC. scMAB-seq also allows the simultaneous high-resolution mapping of sister chromatid exchange (SCE), facilitating the study of this type of genomic rearrangement. Therefore, our study not only establishes new methods for the genomic mapping of active DNA demethylation using limited numbers of cells or single cells but also demonstrates the utilities of the methods in different biological contexts.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Metilação de DNA
Genômica/métodos
Análise de Célula Única/métodos
Troca de Cromátide Irmã
[Mh] Termos MeSH secundário: Animais
Blastômeros/metabolismo
Replicação do DNA
Embrião de Mamíferos
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170912
[Lr] Data última revisão:
170912
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170331
[St] Status:MEDLINE
[do] DOI:10.1101/gad.294843.116


  10 / 8792 MEDLINE  
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[PMID]:28475874
[Au] Autor:Ait Saada A; Teixeira-Silva A; Iraqui I; Costes A; Hardy J; Paoletti G; Fréon K; Lambert SAE
[Ad] Endereço:Institut Curie, PSL Research University, CNRS, UMR3348, F-91405 Orsay, France; University Paris Sud, Paris-Saclay University, CNRS, UMR3348, F-91405 Orsay, France; Labeled Team Fondation pour la Recherche Médicale, UMR3348, F-91405 Orsay, France.
[Ti] Título:Unprotected Replication Forks Are Converted into Mitotic Sister Chromatid Bridges.
[So] Source:Mol Cell;66(3):398-410.e4, 2017 May 04.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Replication stress and mitotic abnormalities are key features of cancer cells. Temporarily paused forks are stabilized by the intra-S phase checkpoint and protected by the association of Rad51, which prevents Mre11-dependent resection. However, if a fork becomes dysfunctional and cannot resume, this terminally arrested fork is rescued by a converging fork to avoid unreplicated parental DNA during mitosis. Alternatively, dysfunctional forks are restarted by homologous recombination. Using fission yeast, we report that Rad52 and the DNA binding activity of Rad51, but not its strand-exchange activity, act to protect terminally arrested forks from unrestrained Exo1-nucleolytic activity. In the absence of recombination proteins, large ssDNA gaps, up to 3 kb long, occur behind terminally arrested forks, preventing efficient fork merging and leading to mitotic sister chromatid bridging. Thus, Rad52 and Rad51 prevent temporarily and terminally arrested forks from degrading and, despite the availability of converging forks, converting to anaphase bridges causing aneuploidy and cell death.
[Mh] Termos MeSH primário: Replicação do DNA
DNA Fúngico/biossíntese
DNA de Cadeia Simples/biossíntese
Mitose/fisiologia
Origem de Replicação
Schizosaccharomyces/metabolismo
Troca de Cromátide Irmã
[Mh] Termos MeSH secundário: Aneuploidia
Cromossomos Fúngicos/genética
Cromossomos Fúngicos/metabolismo
Quebras de DNA de Cadeia Simples
DNA Fúngico/genética
DNA de Cadeia Simples/genética
Proteínas de Ligação a DNA/genética
Proteínas de Ligação a DNA/metabolismo
Viabilidade Microbiana
Rad51 Recombinase/genética
Rad51 Recombinase/metabolismo
Schizosaccharomyces/genética
Schizosaccharomyces/crescimento & desenvolvimento
Proteínas de Schizosaccharomyces pombe/genética
Proteínas de Schizosaccharomyces pombe/metabolismo
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VIDEO-AUDIO MEDIA
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Single-Stranded); 0 (DNA-Binding Proteins); 0 (Rad51 protein, S pombe); 0 (Schizosaccharomyces pombe Proteins); 0 (rad52 protein, S pombe); EC 2.7.7.- (Rad51 Recombinase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE



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