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  1 / 8826 MEDLINE  
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[PMID]:29348659
[Au] Autor:van Wietmarschen N; Merzouk S; Halsema N; Spierings DCJ; Guryev V; Lansdorp PM
[Ad] Endereço:European Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
[Ti] Título:BLM helicase suppresses recombination at G-quadruplex motifs in transcribed genes.
[So] Source:Nat Commun;9(1):271, 2018 01 18.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bloom syndrome is a cancer predisposition disorder caused by mutations in the BLM helicase gene. Cells from persons with Bloom syndrome exhibit striking genomic instability characterized by excessive sister chromatid exchange events (SCEs). We applied single-cell DNA template strand sequencing (Strand-seq) to map the genomic locations of SCEs. Our results show that in the absence of BLM, SCEs in human and murine cells do not occur randomly throughout the genome but are strikingly enriched at coding regions, specifically at sites of guanine quadruplex (G4) motifs in transcribed genes. We propose that BLM protects against genome instability by suppressing recombination at sites of G4 structures, particularly in transcribed regions of the genome.
[Mh] Termos MeSH primário: Síndrome de Bloom/genética
Quadruplex G
Neoplasias/etiologia
RecQ Helicases/metabolismo
Troca de Cromátide Irmã
[Mh] Termos MeSH secundário: Animais
Síndrome de Bloom/complicações
Linhagem Celular
Instabilidade Genômica
Seres Humanos
Perda de Heterozigosidade
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
EC 3.6.1.- (Bloom syndrome protein); EC 3.6.4.12 (RecQ Helicases)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180215
[Lr] Data última revisão:
180215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180120
[St] Status:MEDLINE
[do] DOI:10.1038/s41467-017-02760-1


  2 / 8826 MEDLINE  
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[PMID]:28460277
[Au] Autor:Gruber S
[Ad] Endereço:Department of Fundamental Microbiology, University of Lausanne, Bâtiment Biophore, 1015 Lausanne, Switzerland. Electronic address: stephan.gruber@unil.ch.
[Ti] Título:Shaping chromosomes by DNA capture and release: gating the SMC rings.
[So] Source:Curr Opin Cell Biol;46:87-93, 2017 Jun.
[Is] ISSN:1879-0410
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:SMC proteins organize chromosomes to coordinate essential nuclear processes such as gene expression and DNA recombination as well as to segregate chromosomes during cell division. SMC mediated DNA bridging keeps sister chromatids aligned for much of the cell cycle, while the active extrusion of DNA loops by SMC presumably compacts chromosomes. Chromosome superstructure is thus given by the number of DNA linkages and the size of chromosomal DNA loops, which in turn depend on the dynamics of SMC loading and unloading. The latter is regulated by the intrinsic SMC ATPase activity, multiple external factors and post-translational modification. Here, I highlight recent advances in our understanding of DNA capture and release by SMC-with a focus on cohesin.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/metabolismo
Proteínas Cromossômicas não Histona/metabolismo
Cromossomos/metabolismo
Proteínas de Ligação a DNA/metabolismo
Complexos Multiproteicos/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Ciclo Celular/metabolismo
Divisão Celular
Cromátides/metabolismo
DNA/metabolismo
Seres Humanos
Processamento de Proteína Pós-Traducional
Leveduras/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Chromosomal Proteins, Non-Histone); 0 (DNA-Binding Proteins); 0 (Multiprotein Complexes); 0 (cohesins); 0 (condensin complexes); 9007-49-2 (DNA); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  3 / 8826 MEDLINE  
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[PMID]:28911102
[Au] Autor:Nath S; Somyajit K; Mishra A; Scully R; Nagaraju G
[Ad] Endereço:Department of Biochemistry, Indian Institute of Science, Bangalore 560012, India.
[Ti] Título:FANCJ helicase controls the balance between short- and long-tract gene conversions between sister chromatids.
[So] Source:Nucleic Acids Res;45(15):8886-8900, 2017 Sep 06.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The FANCJ DNA helicase is linked to hereditary breast and ovarian cancers as well as bone marrow failure disorder Fanconi anemia (FA). Although FANCJ has been implicated in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR), the molecular mechanism underlying the tumor suppressor functions of FANCJ remains obscure. Here, we demonstrate that FANCJ deficient human and hamster cells exhibit reduction in the overall gene conversions in response to a site-specific chromosomal DSB induced by I-SceI endonuclease. Strikingly, the gene conversion events were biased in favour of long-tract gene conversions in FANCJ depleted cells. The fine regulation of short- (STGC) and long-tract gene conversions (LTGC) by FANCJ was dependent on its interaction with BRCA1 tumor suppressor. Notably, helicase activity of FANCJ was essential for controlling the overall HR and in terminating the extended repair synthesis during sister chromatid recombination (SCR). Moreover, cells expressing FANCJ pathological mutants exhibited defective SCR with an increased frequency of LTGC. These data unravel the novel function of FANCJ helicase in regulating SCR and SCR associated gene amplification/duplications and imply that these functions of FANCJ are crucial for the genome maintenance and tumor suppression.
[Mh] Termos MeSH primário: Proteína BRCA1/genética
Fatores de Transcrição de Zíper de Leucina Básica/genética
Cromátides/química
DNA/genética
Proteínas de Grupos de Complementação da Anemia de Fanconi/genética
Reparo de DNA por Recombinação
[Mh] Termos MeSH secundário: Animais
Proteína BRCA1/metabolismo
Fatores de Transcrição de Zíper de Leucina Básica/metabolismo
Células CHO
Linhagem Celular Tumoral
Cromátides/metabolismo
Cricetulus
DNA/metabolismo
Quebras de DNA de Cadeia Dupla
Desoxirribonucleases de Sítio Específico do Tipo II/farmacologia
Proteínas de Grupos de Complementação da Anemia de Fanconi/metabolismo
Pontos de Checagem da Fase G2 do Ciclo Celular
Regulação da Expressão Gênica
Recombinação Homóloga/efeitos dos fármacos
Seres Humanos
Mutação
Osteoblastos/citologia
Osteoblastos/efeitos dos fármacos
Osteoblastos/metabolismo
Ligação Proteica
Proteínas de Saccharomyces cerevisiae/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BACH1 protein, human); 0 (BRCA1 Protein); 0 (BRCA1 protein, human); 0 (Basic-Leucine Zipper Transcription Factors); 0 (Fanconi Anemia Complementation Group Proteins); 0 (Saccharomyces cerevisiae Proteins); 9007-49-2 (DNA); EC 3.1.21.- (SCEI protein, S cerevisiae); EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171012
[Lr] Data última revisão:
171012
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170916
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkx586


  4 / 8826 MEDLINE  
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[PMID]:28886051
[Au] Autor:Baek IJ; Moss DS; Lustig AJ
[Ad] Endereço:Department of Biochemistry and Molecular Biology, Tulane University Medical Center, New Orleans, Louisiana, United States of America.
[Ti] Título:The mre11 A470 alleles influence the hereditability and the segregation of telosomes in Saccharomyces cerevisiae.
[So] Source:PLoS One;12(9):e0183549, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Telomeres, the nucleoprotein complexes at the termini of linear chromosomes, are essential for the processes of end replication, end protection, and chromatin segregation. The Mre11 complex is involved in multiple cellular roles in DNA repair and structure in the regulation and function of telomere size homeostasis. In this study, we characterize yeast telomere chromatin structure, phenotypic heritability, and chromatin segregation in both wild-type [MRE11] and A470 motif alleles. MRE11 strains confer a telomere size of 300 base pairs of G+T irregular simple sequence repeats. This DNA and a portion of subtelomeric DNA is embedded in a telosome: a MNase-resistant non-nucleosomal particle. Chromatin immunoprecipitation shows a three to four-fold lower occupancy of Mre11A470T proteins than wild-type proteins in telosomes. Telosomes containing the Mre11A470T protein confer a greater resistance to MNase digestion than wild-type telosomes. The integration of a wild-type MRE11 allele into an ectopic locus in the genome of an mre11A470T mutant and the introduction of an mre11A470T allele at an ectopic site in a wild-type strain lead to unexpectedly differing results. In each case, the replicated sister chromatids inherit telosomes containing only the protein encoded by the genomic mre11 locus, even in the presence of protein encoded by the opposing ectopic allele. We hypothesize that the telosome segregates by a conservative mechanism. These data support a mechanism for the linkage between sister chromatid replication and maintenance of either identical mutant or identical wild-type telosomes after replication of sister chromatids. These data suggest the presence of an active mechanism for chromatin segregation in yeast.
[Mh] Termos MeSH primário: Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/metabolismo
Telômero/genética
Telômero/metabolismo
[Mh] Termos MeSH secundário: Alelos
Cromátides/genética
Cromátides/metabolismo
Imunoprecipitação da Cromatina
Mutação/genética
Proteínas de Saccharomyces cerevisiae/genética
Proteínas de Saccharomyces cerevisiae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Saccharomyces cerevisiae Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183549


  5 / 8826 MEDLINE  
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[PMID]:28880740
[Au] Autor:Doukali H; Ben Salah G; Ben Rhouma B; Hajjaji M; Jaouadi A; Belguith-Mahfouth N; Masmoudi ML; Ammar-Keskes L; Kamoun H
[Ad] Endereço:a Laboratory of Human Molecular Genetics, Faculty of Medicine , Sfax University , Sfax , Tunisia.
[Ti] Título:Cytogenetic monitoring of hospital staff exposed to ionizing radiation: optimize protocol considering DNA repair genes variability.
[So] Source:Int J Radiat Biol;93(11):1283-1288, 2017 Nov.
[Is] ISSN:1362-3095
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Chronic occupational exposure to ionizing radiation (IR) induces a wide spectrum of DNA damages. The aim of this study was to assess the frequencies of micronucleus (MN), sister chromatid exchanges (SCE) and to evaluate their association with XRCC1 399 Arg/Gln and XRCC3 241 Thr/Met polymorphisms in Hospital staff occupationally exposed to IR. MATERIALS AND METHODS: A questionnaire followed by a cytogenetic analysis was concluded for each subject in our study. The exposed subjects were classified into two groups based on duration of employment (Group I < 15 years; Group II ≥15years). The genotypes of all individuals (subjects and controls) were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). RESULTS: DNA damage frequencies were significantly greater in IR workers compared with controls (p < .05). However, no association arised between XRCC1 399 Arg/Gln and XRCC3 241 Thr/Met polymorphisms, on one hand, and the severity of DNA damages in the studied cohort of Tunisian population, on the other hand. CONCLUSION: Our data provide evidence for an obvious genotoxic effect associated with IR exposure and reinforce the high sensitivity of cytogenetic assays for biomonitoring of occupationally exposed populations. These results indicate that workers exposed to IR should have periodic monitoring, along their exposure. The variants, rs25487 and rs861539, of XRCC1 and XRCC3 genes have obvious functional effects. Paradoxically, these variants are not associated with the severity of damages, according to used assays, in the studied cohort of Tunisian population, unlike other studies.
[Mh] Termos MeSH primário: Análise Citogenética
Reparo do DNA/genética
Reparo do DNA/efeitos da radiação
Hospitais
Exposição Ocupacional/efeitos adversos
Exposição Ocupacional/análise
Polimorfismo Genético/efeitos da radiação
[Mh] Termos MeSH secundário: Adulto
Feminino
Técnicas de Genotipagem
Seres Humanos
Masculino
Testes para Micronúcleos
Meia-Idade
Troca de Cromátide Irmã/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1080/09553002.2017.1377361


  6 / 8826 MEDLINE  
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[PMID]:28817615
[Au] Autor:Luaces JP; Rossi LF; Chirino MG; Browne M; Merani MS; Mudry MD
[Ad] Endereço:Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, Paraguay, Piso 10 Lab. 6, Ciudad Autónoma de Buenos Aires, Argentina.
[Ti] Título:Genotoxic effects of Roundup Full II® on lymphocytes of Chaetophractus villosus (Xenarthra, Mammalia): In vitro studies.
[So] Source:PLoS One;12(8):e0182911, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In Argentina, Chaetophractus villosus has a wide distribution that overlaps with agricultural areas where soybean is the predominant crop. In such areas the pesticide Roundup Full II® (RU) is widely applied. The genotoxic effect of its active ingredient glyphosate (RU is 66.2% glyphosate) on the peripheral blood lymphocytes of C. villosus was tested over a range of concentrations (280, 420, 560, 1120 µmol/L). Culture medium without glyphosate served as negative control, while medium containing mitomycin C served as positive control. Genetic damage was characterized in terms of the percentage of cells with chromosome aberrations (CA), the mean number of sister chromatid exchanges (SCE) per cell, and the modification of cell proliferation kinetics via the calculation of the replication index. Significant increases (p < 0.0001) were seen in the CA frequency and the mean number of SCEs per cell compared to negative controls at all the RU concentrations tested. Chromatid breaks, the only form of CA observed, under the 560 µmol/L RU conditions and in presence of mitomycin C were four to five times more common than at lower concentrations, while no viable cells were seen in the 1120 µmol/L treatment. The mean number of SCEs per cell was significantly higher under the 280 µmol/L RU conditions than the 420 or 560 µmol/L RU conditions; cells cultivated in the presence of MMC also showed significantly more SCEs. All the RU concentrations tested (except in the 1120 µmol/L RU treatment [no viable cells]) induced a significant reduction in the replication index (p < 0.0001). The present results confirm the genotoxic effects of RU on C. villosus lymphocytes in vitro, strongly suggesting that exposure to RU could induce DNA damage in C. villosus wildlife.
[Mh] Termos MeSH primário: Dano ao DNA
Glicina/análogos & derivados
Linfócitos/efeitos dos fármacos
Praguicidas/toxicidade
Xenartros/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Quebra Cromossômica
Replicação do DNA
Feminino
Glicina/efeitos adversos
Glicina/toxicidade
Masculino
Praguicidas/efeitos adversos
Troca de Cromátide Irmã
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pesticides); 4632WW1X5A (glyphosate); TE7660XO1C (Glycine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170818
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182911


  7 / 8826 MEDLINE  
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[PMID]:28797117
[Au] Autor:Singh G; Da Ines O; Gallego ME; White CI
[Ad] Endereço:Génétique, Reproduction et Dévelopement, UMR CNRS 6293 - INSERM U1103 - Université Cleront Auvergne Campus Universitaire des Cézeaux, Aubiere, France.
[Ti] Título:Analysis of the impact of the absence of RAD51 strand exchange activity in Arabidopsis meiosis.
[So] Source:PLoS One;12(8):e0183006, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ploidy of eukaryote gametes must be halved to avoid doubling of numbers of chromosomes with each generation and this is carried out by meiosis, a specialized cell division in which a single chromosomal replication phase is followed by two successive nuclear divisions. With some exceptions, programmed recombination ensures the proper pairing and distribution of homologous pairs of chromosomes in meiosis and recombination defects thus lead to sterility. Two highly related recombinases are required to catalyse the key strand-invasion step of meiotic recombination and it is the meiosis-specific DMC1 which is generally believed to catalyse the essential non-sister chromatid crossing-over, with RAD51 catalysing sister-chromatid and non-cross-over events. Recent work in yeast and plants has however shown that in the absence of RAD51 strand-exchange activity, DMC1 is able to repair all meiotic DNA breaks and surprisingly, that this does not appear to affect numbers of meiotic cross-overs. In this work we confirm and extend this conclusion. Given that more than 95% of meiotic homologous recombination in Arabidopsis does not result in inter-homologue crossovers, Arabidopsis is a particularly sensitive model for testing the relative importance of the two proteins-even minor effects on the non-crossover event population should produce detectable effects on crossing-over. Although the presence of RAD51 protein provides essential support for the action of DMC1, our results show no significant effect of the absence of RAD51 strand-exchange activity on meiotic crossing-over rates or patterns in different chromosomal regions or across the whole genome of Arabidopsis, strongly supporting the argument that DMC1 catalyses repair of all meiotic DNA breaks, not only non-sister cross-overs.
[Mh] Termos MeSH primário: Proteínas de Arabidopsis/metabolismo
Arabidopsis/citologia
Quebras de DNA
Rad51 Recombinase/metabolismo
[Mh] Termos MeSH secundário: Arabidopsis/genética
Arabidopsis/metabolismo
Proteínas de Arabidopsis/genética
Cromátides/genética
Cromátides/metabolismo
Reparo do DNA
Recombinação Homóloga
Meiose
Rad51 Recombinase/genética
Recombinação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Arabidopsis Proteins); EC 2.7.7.- (Rad51 Recombinase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0183006


  8 / 8826 MEDLINE  
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[PMID]:28781233
[Au] Autor:Krishnan S; Smits AH; Vermeulen M; Reinberg D
[Ad] Endereço:Howard Hughes Medical Institute, New York University Langone School of Medicine, New York, NY 10016, USA; Department of Biochemistry and Molecular Pharmacology, New York University Langone School of Medicine, New York, NY 10016, USA.
[Ti] Título:Phospho-H1 Decorates the Inter-chromatid Axis and Is Evicted along with Shugoshin by SET during Mitosis.
[So] Source:Mol Cell;67(4):579-593.e6, 2017 Aug 17.
[Is] ISSN:1097-4164
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Precise control of sister chromatid separation during mitosis is pivotal to maintaining genomic integrity. Yet, the regulatory mechanisms involved are not well understood. Remarkably, we discovered that linker histone H1 phosphorylated at S/T18 decorated the inter-chromatid axial DNA on mitotic chromosomes. Sister chromatid resolution during mitosis required the eviction of such H1S/T18ph by the chaperone SET, with this process being independent of and most likely downstream of arm-cohesin dissociation. SET also directed the disassembly of Shugoshins in a polo-like kinase 1-augmented manner, aiding centromere resolution. SET ablation compromised mitotic fidelity as evidenced by unresolved sister chromatids with marked accumulation of H1S/T18ph and centromeric Shugoshin. Thus, chaperone-assisted eviction of linker histones and Shugoshins is a fundamental step in mammalian mitotic progression. Our findings also elucidate the functional implications of the decades-old observation of mitotic linker histone phosphorylation, serving as a paradigm to explore the role of linker histones in bio-signaling processes.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
Cromátides/metabolismo
Chaperonas de Histonas/metabolismo
Histonas/metabolismo
Mitose
Proteínas Oncogênicas/metabolismo
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Animais
Proteínas de Ciclo Celular/genética
Cromátides/genética
Segregação de Cromossomos
Fibroblastos/metabolismo
Células HEK293
Chaperonas de Histonas/genética
Seres Humanos
Camundongos
Proteínas Oncogênicas/genética
Fosforilação
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas Proto-Oncogênicas/genética
Proteínas Proto-Oncogênicas/metabolismo
Interferência de RNA
Epitélio Pigmentado da Retina/metabolismo
Transdução de Sinais
Fatores de Transcrição/genética
Transfecção
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cell Cycle Proteins); 0 (Histone Chaperones); 0 (Histones); 0 (Oncogene Proteins); 0 (Proto-Oncogene Proteins); 0 (SET protein, human); 0 (SET protein, mouse); 0 (SGOL1 protein, human); 0 (Transcription Factors); 0 (shugoshin protein, mouse); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 2.7.11.1 (polo-like kinase 1)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170808
[St] Status:MEDLINE


  9 / 8826 MEDLINE  
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[PMID]:28719910
[Au] Autor:Bustamante FO; Aliyeva-Schnorr L; Fuchs J; Beier S; Houben A
[Ad] Endereço:Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
[Ti] Título:Correlating the Genetic and Physical Map of Barley Chromosome 3H Revealed Limitations of the FISH-Based Mapping of Nearby Single-Copy Probes Caused by the Dynamic Structure of Metaphase Chromosomes.
[So] Source:Cytogenet Genome Res;152(2):90-96, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Genetic maps are based on the recombination frequency of molecular markers which often show different positions in comparison to the corresponding physical maps. To decipher the position and order of DNA sequences genetically mapped to terminal and interstitial regions of barley (Hordeum vulgare) chromosome 3H, fluorescence in situ hybridization (FISH) on mitotic metaphase chromosomes was performed with 16 genomic single-copy probes derived from fingerprinted BAC contigs. Long genetic distances at subterminal regions translated into short physical distances, confirming that recombination events occur more often at distal regions of chromosome 3H. Nonoverlapping FISH signals were frequently obtained for probes with a physical distance of at least 30-60 kb. Only 8% of the analyzed chromosomes showed a symmetric order of FISH signals on both sister chromatids. Due to the dynamic packing of metaphase chromatin, the order of 2 adjacent single-copy signals along the chromosome arms outside the (peri)centromeric region can only reliably be determined if the cytological distance is approximately 3%, corresponding to 21.6 Mb.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Cromossomos de Plantas/genética
Dosagem de Genes
Hordeum/genética
Hibridização in Situ Fluorescente/métodos
Metáfase/genética
Mapeamento Físico do Cromossomo/métodos
[Mh] Termos MeSH secundário: Pareamento de Bases/genética
Cromátides/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1159/000478631


  10 / 8826 MEDLINE  
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[PMID]:28574395
[Au] Autor:Jokhadze T; Gaiozishvili M; Buadze T; Sigua T; Namchelvadze E; Lezhava T
[Ad] Endereço:1Ivane Javakhishvili Tbilisi State University, Department of Genetics; 2Institute of Physics, Laboratory of Biological Systems, Tbilisi, Georgia.
[Ti] Título:[EVALUATION OF GENOMIC PARAMETERS IN DUCTAL BREAST CANCER PATIENTS AND THE ABILITY OF IT'S CORRECTION].
[So] Source:Georgian Med News;(265):120-125, 2017 Apr.
[Is] ISSN:1512-0112
[Cp] País de publicação:Georgia (Republic)
[La] Idioma:rus
[Ab] Resumo:The level of DNA single strand breaks, chromosomal abnormalities and sister chromatid exchanges and the possibility of its normalization with oligopeptide bioregulator Livagen and cobalt ions in the lymphocyte culture from patients with breast cancer have been studied. The results show that the genome of ductal breast cancer patients is characterized by the high density of DNA single strand breaks, high frequency of chromosomal abnormalities and increased levels of chromatin condensation. The usage of Livagen and cobalt in the form of modifying agents has a protective effect by all studied parameters. The obtained results allow us to conclude that research of lymphocytes of ductal breast cancer patients using the analysis conducted by us, can be useful in assessing the therapeutic effect in the treatment of breast cancer patients.
[Mh] Termos MeSH primário: Neoplasias da Mama/genética
Carcinoma Ductal de Mama/genética
[Mh] Termos MeSH secundário: Neoplasias da Mama/imunologia
Carcinoma Ductal de Mama/imunologia
Células Cultivadas
Aberrações Cromossômicas/efeitos dos fármacos
Cobalto/farmacologia
Quebras de DNA de Cadeia Simples/efeitos dos fármacos
Feminino
Seres Humanos
Linfócitos/efeitos dos fármacos
Linfócitos/imunologia
Oligopeptídeos/farmacologia
Troca de Cromátide Irmã/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Livagen); 0 (Oligopeptides); 3G0H8C9362 (Cobalt); EVS87XF13W (cobaltous chloride)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE



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