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Pesquisa : Cucumis and sativus [Palavras]
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  1 / 2695 MEDLINE  
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[PMID]:29523804
[Au] Autor:Li YT; Liang Y; Li YN; Che XK; Zhao SJ; Zhang ZS; Gao HY
[Ad] Endereço:State Key Lab of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, Shandong Province, China.
[Ti] Título:Mechanisms by which Bisphenol A affect the photosynthetic apparatus in cucumber (Cucumis sativus L.) leaves.
[So] Source:Sci Rep;8(1):4253, 2018 Mar 09.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bisphenol A (BPA), a widely distributed pollutant, suppresses photosynthesis in leaves. In previous studies on higher plants, the plants were treated by BPA through irrigation to root. This method cannot distinguish whether the BPA directly suppresses photosynthesis in leaves, or indirectly influences photosynthesis through affecting the function of root. Here, only the leaves but not the roots of cucumber were infiltrated with BPA solution. The photosystem II and I (PSII, PSI) were insensitive to BPA under darkness. BPA aggravated the PSII but not the PSI photoinhibition under light. BPA also inhibited CO assimilation, and the effect of BPA on PSII photoinhibition disappeared when the CO assimilation was blocked. The H O accumulated in BPA-treated leaves under light. And the BPA-caused PSII photoinhibition was prevented under low (2%) O . We also proved that the BPA-caused PSII photoinhibition depend on the turnover of D1 protein. In conclusion, this study proved that BPA could directly suppress photosynthesis in leaves, however, BPA does not damage PSII directly, but inhibits CO assimilation and over-reduces the electron transport chain under light, which increases the production of reactive oxygen species (H O ), the over-accumulated ROS inhibits the turnover of D1 protein and consequently aggravates PSII photoinhibition.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180310
[Lr] Data última revisão:
180310
[St] Status:In-Data-Review
[do] DOI:10.1038/s41598-018-22486-4


  2 / 2695 MEDLINE  
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[PMID]:29461868
[Au] Autor:Zhou Y; Hu L; Jiang L; Liu S
[Ad] Endereço:a College of Science, Jiangxi Agricultural University, Nanchang Economic and Technological Development District, Nanchang, Jiangxi 330045, China.
[Ti] Título:Genome-wide identification, characterization, and transcriptional analysis of the metacaspase gene family in cucumber (Cucumis sativus).
[So] Source:Genome;61(3):187-194, 2018 Mar.
[Is] ISSN:1480-3321
[Cp] País de publicação:Canada
[La] Idioma:eng
[Ab] Resumo:Metacaspase (MC), a family of caspase-like proteins, plays vital roles in regulating programmed cell death (PCD) during development and in response to stresses in plants. In this study, five MC genes (designated as CsMC1 to CsMC5) were identified in the cucumber (Cucumis sativus) genome. Sequence analysis revealed that CsMC1-CsMC3 belong to type I MC proteins, while CsMC4 and CsMC5 are type II MC proteins. Phylogenetic tree and conserved motif analysis of MC proteins indicated that these proteins can be classified into two groups, which are correlated with the types of these MC proteins. Gene structure analysis demonstrated that type I CsMC genes contain 4-7 introns, while all type II CsMC genes harbor one intron. In addition, many hormone-, stress-, and development-related cis-elements were identified in the promoter regions of CsMC genes. Expression analysis using RNA-seq data revealed that CsMC genes have distinct expression patterns in various tissues and developmental stages. qRT-PCR results showed that the transcript levels of CsMC genes could be regulated by various abiotic stresses such as NaCl, PEG, and cold. These results demonstrate that the cucumber MC gene family may function in tissue development and plant stress responses.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180307
[Lr] Data última revisão:
180307
[St] Status:In-Process
[do] DOI:10.1139/gen-2017-0174


  3 / 2695 MEDLINE  
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[PMID]:29279005
[Au] Autor:Sharma KK; Bhushan VS; Rao CS; Reddy KN; Banerjee H; Mandal S; Singh B; Battu RS; Jyot G; Sahoo SK; Mohapatra S; Lekha S; Manikrao G; Radhika B; Tripathy V; Yadav R; Shukla P; Patel AN; Singh G; Devi S; Pandey P; Gautam R; Kalra S; Gupta R; Singh G; Gopal M; Walia S
[Ad] Endereço:a All India Network Project on Pesticide Residues , ICAR-Indian Agricultural Research Institute , New Delhi , India.
[Ti] Título:Persistence, dissipation and consumer risk assessment of a combination formulation of flubendiamide and deltamethrin on cucumber.
[So] Source:Food Addit Contam Part A Chem Anal Control Expo Risk Assess;35(3):498-511, 2018 Mar.
[Is] ISSN:1944-0057
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Multi-location supervised field trials were conducted in India at four locations of the All India Network Project (AINP) on Pesticide Residues to study the persistence, dissipation and risk assessment of flubendiamide and deltamethrin on cucumber (Cucumis sativus). Residues of flubendiamide and deltamethrin on cucumber resulting from three spray applications of a combination formulation (flubendiamide 90% + deltamethrin 60%, 150 SC) at recommended (22.5 + 15 g a.i./ha) and double the recommended (45 + 30 g a.i./ha) dose were analysed. On the basis of persistence and dissipation studies, the half- life (T ) of flubendiamide on cucumber varied from 1.40 to 2.98 (recommended dose) and 1.55 to 2.76 days (double the recommended dose), while that of deltamethrin ranged from 2.5 to 4.9 (recommended dose) and 2.7 to 3.9 days (double the recommended dose) at the four locations. On the basis of supervised field trial data and using OECD calculator, MRLs in the combination product of 3 mg kg for flubendiamide and 1.5 mg kg for deltamethrin has been proposed for consideration by the Food Safety and Standards Authority of India (FSSAI). Codex, EU and EPA have fixed MRL of 0.2 mg kg for flubendiamide and deltamethrin.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[St] Status:In-Process
[do] DOI:10.1080/19440049.2017.1416678


  4 / 2695 MEDLINE  
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[PMID]:29485125
[Au] Autor:Joseph JT; Poolakkalody NJ; Shah JM
[Ad] Endereço:Department of Plant Science, Central University of Kerala, Padannakkad, Kasaragod 671 314, India.
[Ti] Título:Plant reference genes for development and stress response studies.
[So] Source:J Biosci;43(1):173-187, 2018 Mar.
[Is] ISSN:0973-7138
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Many reference genes are used by different laboratories for gene expression analyses to indicate the relative amount of input RNA/DNA in the experiment. These reference genes are supposed to show least variation among the treatments and with the control sets in a given experiment. However, expression of reference genes varies significantly from one set of experiment to the other. Thus, selection of reference genes depends on the experimental conditions. Sometimes the average expression of two or three reference genes is taken as standard. This review consolidated the details of about 120 genes attempted for normalization during comparative expression analysis in 16 different plants. Plant species included in this review are Arabidopsis thaliana, cotton (Gossypium hirsutum), tobacco (Nicotiana benthamiana and N. tabacum), soybean (Glycine max), rice (Oryza sativa), blueberry (Vaccinium corymbosum), tomato (Solanum lycopersicum), wheat (Triticum aestivum), potato (Solanum tuberosum), sugar cane (Saccharum sp.), carrot (Daucus carota), coffee (Coffea arabica), cucumber (Cucumis sativus), kiwi (Actinidia deliciosa) and grape (Vitis vinifera). The list includes model and cultivated crop plants from both monocot and dicot classes. We have categorized plant-wise the reference genes that have been used for expression analyses in any or all of the four different conditions such as biotic stress, abiotic stress, developmental stages and various organs and tissues, reported till date. This review serves as a guide during the reference gene hunt for gene expression analysis studies.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[St] Status:In-Process


  5 / 2695 MEDLINE  
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[PMID]:29325723
[Au] Autor:Toporkova YY; Gorina SS; Bessolitsyna EK; Smirnova EO; Fatykhova VS; Brühlmann F; Ilyina TM; Mukhtarova LS; Grechkin AN
[Ad] Endereço:Kazan Institute of Biochemistry and Biophysics, Russian Academy of Sciences, P.O. Box 30, Kazan 420111, Russia. Electronic address: toporkova@kibb.knc.ru.
[Ti] Título:Double function hydroperoxide lyases/epoxyalcohol synthases (CYP74C) of higher plants: identification and conversion into allene oxide synthases by site-directed mutagenesis.
[So] Source:Biochim Biophys Acta;1863(4):369-378, 2018 Apr.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The CYP74C subfamily of fatty acid hydroperoxide transforming enzymes includes hydroperoxide lyases (HPLs) and allene oxide synthases (AOSs). This work reports a new facet of the putative CYP74C HPLs. Initially, we found that the recombinant CYP74C13_MT (Medicago truncatula) behaved predominantly as the epoxyalcohol synthase (EAS) towards the 9(S)-hydroperoxide of linoleic acid. At the same time, the CYP74C13_MT mostly possessed the HPL activity towards the 13(S)-hydroperoxides of linoleic and α-linolenic acids. To verify whether this dualistic behaviour of CYP74C13_MT is occasional or typical, we also examined five similar putative HPLs (CYP74C). These were CYP74C4_ST (Solanum tuberosum), CYP74C2 (Cucumis melo), CYP74C1_CS and CYP74C31 (both of Cucumis sativus), and CYP74C13_GM (Glycine max). All tested enzymes behaved predominantly as EAS toward 9-hydroperoxide of linoleic acid. Oxiranyl carbinols such as (9S,10S,11S,12Z)-9,10-epoxy-11-hydroxy-12-octadecenoic acids were the major EAS products. Besides, the CYP74C31 possessed an additional minor 9-AOS activity. The mutant forms of CYP74C13_MT, CYP74C1_CS, and CYP74C31 with substitutions at the catalytically essential domains, namely the "hydroperoxide-binding domain" (I-helix), or the SRS-1 domain near the N-terminus, showed strong AOS activity. These HPLs to AOSs conversions were observed for the first time. Until now a large part of CYP74C enzymes has been considered as 9/13-HPLs. Notwithstanding, these results show that all studied putative CYP74C HPLs are in fact the versatile HPL/EASs that can be effortlessly mutated into specific AOSs.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180304
[Lr] Data última revisão:
180304
[St] Status:In-Data-Review


  6 / 2695 MEDLINE  
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[PMID]:29330162
[Au] Autor:Mróz TL; Eves-van den Akker S; Bernat A; Skarzynska A; Pryszcz L; Olberg M; Havey MJ; Bartoszewski G
[Ad] Endereço:Department of Plant Genetics Breeding and Biotechnology, Faculty of Horticulture Biotechnology and Landscape Architecture, Warsaw University of Life Sciences, 02-776, Poland.
[Ti] Título:Transcriptome Analyses of Mosaic (MSC) Mitochondrial Mutants of Cucumber in a Highly Inbred Nuclear Background.
[So] Source:G3 (Bethesda);8(3):953-965, 2018 Mar 02.
[Is] ISSN:2160-1836
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cucumber ( L.) has a large, paternally transmitted mitochondrial genome. Cucumber plants regenerated from cell cultures occasionally show paternally transmitted mosaic (MSC) phenotypes, characterized by slower growth, chlorotic patterns on the leaves and fruit, lower fertility, and rearrangements in their mitochondrial DNAs (mtDNAs). MSC lines 3, 12, and 16 originated from different cell cultures all established using the highly inbred, wild-type line B. These MSC lines possess different rearrangements and under-represented regions in their mtDNAs. We completed RNA-seq on normalized and non-normalized cDNA libraries from MSC3, MSC12, and MSC16 to study their nuclear gene-expression profiles relative to inbred B. Results from both libraries indicated that gene expression in MSC12 and MSC16 were more similar to each other than MSC3. Forty-one differentially expressed genes (DEGs) were upregulated and one downregulated in the MSC lines relative to B. Gene functional classifications revealed that more than half of these DEGs are associated with stress-response pathways. Consistent with this observation, we detected elevated levels of hydrogen peroxide throughout leaf tissue in all MSC lines compared to wild-type line B. These results demonstrate that independently produced MSC lines with different mitochondrial polymorphisms show unique and shared nuclear responses. This study revealed genes associated with stress response that could become selection targets to develop cucumber cultivars with increased stress tolerance, and further support of cucumber as a model plant to study nuclear-mitochondrial interactions.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180303
[Lr] Data última revisão:
180303
[St] Status:In-Data-Review
[do] DOI:10.1534/g3.117.300321


  7 / 2695 MEDLINE  
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[PMID]:29495578
[Au] Autor:Trejo-Moreno C; Méndez-Martínez M; Zamilpa A; Jiménez-Ferrer E; Perez-Garcia MD; Medina-Campos ON; Pedraza-Chaverri J; Santana MA; Esquivel-Guadarrama FR; Castillo A; Cervantes-Torres J; Fragoso G; Rosas-Salgado G
[Ad] Endereço:Instituto de Investigación en Ciencias Básicas y Aplicadas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Cuernavaca, Morelos CP 62209, Mexico. trejomc@hotmail.com.
[Ti] Título:Cucumis sativus Aqueous Fraction Inhibits Angiotensin II-Induced Inflammation and Oxidative Stress In Vitro.
[So] Source:Nutrients;10(3), 2018 Feb 28.
[Is] ISSN:2072-6643
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Inflammation and oxidative stress play major roles in endothelial dysfunction, and are key factors in the progression of cardiovascular diseases. The aim of this study was to evaluate in vitro the effect of three subfractions (SFs) from the aqueous fraction to reduce inflammatory factors and oxidative stress induced by angiotensin II (Ang II) in human microvascular endothelial cells-1 (HMEC-1) cells. The cells were cultured with different concentrations of Ang II and 0.08 or 10 µg/mL of SF1, SF2, or SF3, or 10 µmol of losartan as a control. IL-6 (Interleukin 6) concentration was quantified. To identify the most effective SF combinations, HMEC-1 cells were cultured as described above in the presence of four combinations of SF1 and SF3. Then, the effects of the most effective combination on the expression of adhesion molecules, the production of reactive oxygen species (ROS), and the bioavailability of nitric oxide (NO) were evaluated. Finally, a mass spectrometry analysis was performed. Both SF1 and SF3 subfractions decreased the induction of IL-6 by Ang II, and C4 (SF1 and SF3, 10 µg/mL each) was the most effective combination to inhibit the production of IL-6. Additionally, C4 prevented the expression of adhesion molecules, reduced the production of ROS, and increased the bioavailability of NO. Glycine, arginine, asparagine, lysine, and aspartic acid were the main components of both subfractions. These results demonstrate that C4 has anti-inflammatory and antioxidant effects.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[St] Status:In-Process


  8 / 2695 MEDLINE  
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[PMID]:29492617
[Au] Autor:Xie Q; Liu P; Shi L; Miao H; Bo K; Wang Y; Gu X; Zhang S
[Ad] Endereço:Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Combined fine mapping, genetic diversity, and transcriptome profiling reveals that the auxin transporter gene ns plays an important role in cucumber fruit spine development.
[So] Source:Theor Appl Genet;, 2018 Feb 28.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Map-based cloning was used to identify the ns gene, which was involved in the formation of cucumber numerous fruit spines together with other genes under regulation by plant hormone signal transduction. The cucumber (Cucumis sativus) fruit spine density has an important impact on the commercial value. However, little is known about the regulatory mechanism for the fruit spine formation. Here, we identified NUMEROUS SPINES (NS), which regulate fruit spine development by modulating the Auxin signaling pathway. We fine-mapped the ns using a 2513 F population derived from NCG122 (numerous fruit spines line) and NCG121 (few fruit spines line), and showed that NS encoded auxin transporter-like protein 3. Genetic diversity analysis of the NS gene in natural populations revealed that one SNP and one InDel in the coding region of ns are co-segregated with the fruit spine density. The NS protein sequence was highly conserved among plants, but its regulation of fruit spine development in cucumber seems to be a novel function. Transcriptome profiling indicated that the plant hormone signal transduction-related genes were highly enriched in the up-regulated genes in NCG122 versus NCG121. Moreover, expression pattern analysis of the auxin signal pathway-related genes in NCG122 versus NCG121 showed that upstream genes of the pathway (like ns candidate gene Csa2M264590) are down-regulated, while the downstream genes are up-regulated. Quantitative reverse transcription PCR confirmed the differential expression during the fruit spine development. Therefore, reduced expression of ns may promote the fruit spine formation. Our findings provide a valuable framework for dissecting the regulatory mechanism for the fruit spine development.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180301
[Lr] Data última revisão:
180301
[St] Status:Publisher
[do] DOI:10.1007/s00122-018-3074-x


  9 / 2695 MEDLINE  
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[PMID]:29488198
[Au] Autor:Chawla S; Patel DJ; Patel SH; Kalasariya RL; Shah PG
[Ad] Endereço:Pesticide Residue Laboratory, All India Network Project on Pesticide Residues, Anand Agricultural University, Anand, Gujarat, 388110, India. suchisood10@yahoo.co.uk.
[Ti] Título:Correction to: Behaviour and risk assessment of fluopyram and its metabolite in cucumber (Cucumis sativus) fruit and in soil.
[So] Source:Environ Sci Pollut Res Int;, 2018 Feb 27.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Unfortunately, the original publication of this paper contains a mistake. The correct name of the 3rd Author is Sunny H. Patel. The original article has been corrected.
[Pt] Tipo de publicação:PUBLISHED ERRATUM
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[St] Status:Publisher
[do] DOI:10.1007/s11356-018-1603-4


  10 / 2695 MEDLINE  
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[PMID]:29429109
[Au] Autor:Chawla S; Patel DJ; Patel SH; Kalasariya RL; Shah PG
[Ad] Endereço:Pesticide Residue Laboratory, All India Network Project on Pesticide Residues, Anand Agricultural University, Anand, Gujarat, 388110, India. suchisood10@yahoo.co.uk.
[Ti] Título:Behaviour and risk assessment of fluopyram and its metabolite in cucumber (Cucumis sativus) fruit and in soil.
[So] Source:Environ Sci Pollut Res Int;, 2018 Feb 10.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:A field experiment was conducted to estimate residue persistence of fluopyram and its metabolite benzamide in cucumber fruits and soil and their risk assessment in humans and soil environment. Fluopyram (Kafka, 400 SC) was applied as soil drench twice at the interval of 15 days at the rate of 250 (standard dose) and 500 (double dose) g a.i. ha (active ingredient per hectare). Cucumber fruits were collected at 0 (1 h), 1, 3, 5, 7, 10, 15, 20, 30, 40 and 50 days after second application. Soil samples were collected on 15th day after second application. Drench application resulted in detection of residues on the third day in standard dose at the levels of 0.056 mg kg in cucumber fruit. The residue level increased until 20 days reaching 0.092 mg kg followed by decrease to 0.068 mg kg on 30th day after application. In double dose, the residues started accumulating from 0 day with initial levels of 0.093 mg kg and persisted until 30th day. The levels varied between 0.123 and 0.184 mg kg until 15th day of application followed by decrease to 0.127 mg kg by 30th day. The residues reached below determination level (< 0.05 mg kg ) on 40th day in both the doses after second application. The residue of metabolite benzamide was below determination level (< 0.05 mg kg ) at both the doses. Hazard quotient (HQ) for residues levels at 15th and 30th day was less than one (HQ < 1). Hence, a pre-harvest interval of 15 days is suggested. Present data can be used to establish maximum residue limit (MRL) in India. The residue of fluopyram in soil on 15th day and the data on soil adsorption coefficient of fluopyram from literature suggests moderate mobility of fluopyram in soil. However, residues of metabolite of benzamide were not detected in soil. Further studies on translocation of fluopyram in soil over the time can be conducted for better understanding of environmental risk. To our knowledge, this is the first report on residue levels of fluopyram in any crop when applied as soil drench.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[St] Status:Publisher
[do] DOI:10.1007/s11356-018-1439-y



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