Base de dados : MEDLINE
Pesquisa : Degeneração and Retiniana [Palavras]
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  1 / 9957 MEDLINE  
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[PMID]:29231925
[Au] Autor:Zhang L; Zhang X; Zhang G; Pang CP; Leung YF; Zhang M; Zhong W
[Ad] Endereço:Department of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907, USA.
[Ti] Título:Expression profiling of the retina of pde6c, a zebrafish model of retinal degeneration.
[So] Source:Sci Data;4:170182, 2017 12 12.
[Is] ISSN:2052-4463
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Retinal degeneration often affects the whole retina even though the disease-causing gene is specifically expressed in the light-sensitive photoreceptors. The molecular basis of the retinal defect can potentially be determined by gene-expression profiling of the whole retina. In this study, we measured the gene-expression profile of retinas microdissected from a zebrafish pde6c (pde6c) mutant. This retinal-degeneration model not only displays cone degeneration caused by a cone-specific mutation, but also other secondary cellular changes starting from 4 days postfertilization (dpf). To capture the underlying molecular changes, we subjected pde6c and wild-type (WT) retinas at 5 dpf/ 120 h postfertilization (hpf) to RNA sequencing (RNA-Seq) on the Illumina HiSeq 2,000 platform. We also validated the RNA-Seq results by Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR) of seven phototransduction genes. Our analyses indicate that the RNA-Seq dataset was of high quality, and effectively captured the molecular changes in the whole pde6c retina. This dataset will facilitate the characterization of the molecular defects in the pde6c retina at the initial stage of retinal degeneration.
[Mh] Termos MeSH primário: Nucleotídeo Cíclico Fosfodiesterase do Tipo 6/genética
Retina/metabolismo
Degeneração Retiniana/genética
Proteínas de Peixe-Zebra/genética
[Mh] Termos MeSH secundário: Animais
Análise em Microsséries
Transcriptoma
Peixe-Zebra
[Pt] Tipo de publicação:DATASET; JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Zebrafish Proteins); EC 3.1.4.- (Pde6c protein, zebrafish); EC 3.1.4.35 (Cyclic Nucleotide Phosphodiesterases, Type 6)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171213
[St] Status:MEDLINE
[do] DOI:10.1038/sdata.2017.182


  2 / 9957 MEDLINE  
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[PMID]:28744551
[Au] Autor:Halupka KJ; Abbott CJ; Wong YT; Cloherty SL; Grayden DB; Burkitt AN; Sergeev EN; Luu CD; Brandli A; Allen PJ; Meffin H; Shivdasani MN
[Ad] Endereço:NeuroEngineering Laboratory, Department of Biomedical Engineering, The University of Melbourne, Victoria, Australia 2Data61, Commonwealth Scientific and Industrial Research Organisation (CSIRO), New South Wales, Australia 3Bionics Institute, East Melbourne, Victoria, Australia.
[Ti] Título:Neural Responses to Multielectrode Stimulation of Healthy and Degenerate Retina.
[So] Source:Invest Ophthalmol Vis Sci;58(9):3770-3784, 2017 07 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: Simultaneous stimulation of multiple retinal electrodes in normally sighted animals shows promise in improving the resolution of retinal prostheses. However, the effects of simultaneous stimulation on degenerate retinae remain unknown. Therefore, we investigated the characteristics of cortical responses to multielectrode stimulation of the degenerate retina. Methods: Four adult cats were bilaterally implanted with retinal electrode arrays in the suprachoroidal space after unilateral adenosine triphosphate (ATP)-induced retinal photoreceptor degeneration. Functional and structural changes were characterized by using electroretinogram a-wave amplitude and optical coherence tomography. Multiunit activity was recorded from both hemispheres of the visual cortex. Responses to single- and multielectrode stimulation of the ATP-injected and fellow control eyes were characterized and compared. Results: The retinae of ATP-injected eyes displayed structural and functional changes consistent with mid- to late-stage photoreceptor degeneration and remodeling. Responses to multielectrode stimulation of the ATP-injected eyes exhibited shortened latencies, lower saturated spike counts, and higher thresholds, compared to stimulation of the fellow control eyes. Electrical receptive field sizes were significantly larger in the ATP-injected eye than in the control eye, and positively correlated with the extent of degeneration. Conclusions: Significant differences exist between cortical responses to stimulation of healthy and degenerate retinae. Our results highlight the importance of using a retinal degeneration model when evaluating the efficacy of novel stimulation paradigms.
[Mh] Termos MeSH primário: Estimulação Elétrica/métodos
Potenciais Evocados Visuais/fisiologia
Células Fotorreceptoras de Vertebrados/fisiologia
Degeneração Retiniana/fisiopatologia
Córtex Visual/fisiologia
Próteses Visuais
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/toxicidade
Animais
Gatos
Modelos Animais de Doenças
Eletrodos Implantados
Eletrorretinografia
Estimulação Luminosa
Degeneração Retiniana/induzido quimicamente
Degeneração Retiniana/diagnóstico
Tomografia de Coerência Óptica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
8L70Q75FXE (Adenosine Triphosphate)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170727
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-21290


  3 / 9957 MEDLINE  
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[PMID]:29281713
[Au] Autor:Haselier C; Biswas S; Rösch S; Thumann G; Müller F; Walter P
[Ad] Endereço:Department of Ophthalmology, RWTH Aachen University, Aachen, Germany.
[Ti] Título:Correlations between specific patterns of spontaneous activity and stimulation efficiency in degenerated retina.
[So] Source:PLoS One;12(12):e0190048, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Retinal prostheses that are currently used to restore vision in patients suffering from retinal degeneration are not adjusted to the changes occurring during the remodeling process of the retina. Recent studies revealed abnormal rhythmic activity in the retina of genetic mouse models of retinitis pigmentosa. Here we describe this abnormal activity also in a pharmacologically-induced (MNU) mouse model of retinal degeneration. To investigate how this abnormal activity affects the excitability of retinal ganglion cells, we recorded the electrical activity from whole mounted retinas of rd10 mice and MNU-treated mice using a microelectrode array system and applied biphasic current pulses of different amplitude and duration to stimulate ganglion cells electrically. We show that the electrical stimulation efficiency is strongly reduced in degenerated retinas, in particular when abnormal activity such as oscillations and rhythmic firing of bursts of action potentials can be observed. Using a prestimulus pulse sequence, we could abolish rhythmic retinal activity. Under these conditions, the stimulation efficiency was enhanced in a few cases but not in the majority of tested cells. Nevertheless, this approach supports the idea that modified stimulation protocols could help to improve the efficiency of retinal prostheses in the future.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Degeneração Retiniana/fisiopatologia
[Mh] Termos MeSH secundário: Potenciais de Ação
Animais
Estimulação Elétrica
Camundongos
Microeletrodos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180206
[Lr] Data última revisão:
180206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171228
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0190048


  4 / 9957 MEDLINE  
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[PMID]:28460050
[Au] Autor:Dharmat R; Liu W; Ge Z; Sun Z; Yang L; Li Y; Wang K; Thomas K; Sui R; Chen R
[Ad] Endereço:Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas, United States 2Human Genome Sequencing Center, Baylor College of Medicine, Houston, Texas, United States.
[Ti] Título:IFT81 as a Candidate Gene for Nonsyndromic Retinal Degeneration.
[So] Source:Invest Ophthalmol Vis Sci;58(5):2483-2490, 2017 05 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: IFT81, a core component of the IFT-B complex, involved in the bidirectional transport of ciliary proteins, has been recently implicated in syndromic ciliopathies. However, none of the IFT-B core complex proteins have been associated with nonsyndromic retinal dystrophies. Given the importance of ciliary transport in photoreceptor function and structural maintenance, we sought to investigate the impact of IFT (intraflagellar transport) mutations in nonsyndromic retinopathies. Methods: Whole exome sequencing was performed on 50 cone-rod dystrophy (CRD) patients that were previously screened for mutations in known retinal disease genes. The impact of candidate mutation was studied using in vitro cell system and in vivo zebrafish assay to determine the pathogenicity of the variant. Results: Compound heterozygous mutations in IFT81, including one nonsense (c.1213C>T, p.R405*) and one missense variant (c.1841T>C, p.L614P), were identified in a nonsyndromic CRD proband. Extensive functional analyses of the missense variant in cell culture and zebrafish strongly suggests its pathogenic nature. Loss of IFT81 impairs ciliogenesis and, interestingly, the missense variant displayed significantly reduced rescue of ciliogenesis in the IFT81 knockdown in vitro system. Consistently, dramatic reduction of rescue efficiency of the ift81 mutant zebrafish embryo by mRNA with the missense variant was observed, further supporting its pathogenicity. Conclusions: Consistent with the function of the IFT-B complex in the maintenance of photoreceptor cilium, we report a case of mutations in a core IFT-B protein, IFT81. This represents the first report of mutations in IFT81 as a candidate gene for nonsyndromic retinal dystrophy, hence expanding the phenotype spectrum of IFT-B components.
[Mh] Termos MeSH primário: DNA/genética
Proteínas Musculares/genética
Mutação
Retina/metabolismo
Degeneração Retiniana/genética
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Códon sem Sentido
Análise Mutacional de DNA
Modelos Animais de Doenças
Feminino
Seres Humanos
Masculino
Proteínas Musculares/metabolismo
Fenótipo
Retina/patologia
Degeneração Retiniana/metabolismo
Degeneração Retiniana/patologia
Peixe-Zebra
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Codon, Nonsense); 0 (IFT81 protein, human); 0 (Muscle Proteins); 9007-49-2 (DNA)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180124
[Lr] Data última revisão:
180124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.16-19133


  5 / 9957 MEDLINE  
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[PMID]:28452939
[Au] Autor:Cehofski LJ; Honoré B; Vorum H
[Ad] Endereço:Department of Ophthalmology, Aalborg University Hospital, Hobrovej 18-22, 9000 Aalborg, Denmark. L.cehofski@rn.dk.
[Ti] Título:A Review: Proteomics in Retinal Artery Occlusion, Retinal Vein Occlusion, Diabetic Retinopathy and Acquired Macular Disorders.
[So] Source:Int J Mol Sci;18(5), 2017 Apr 28.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Retinal artery occlusion (RAO), retinal vein occlusion (RVO), diabetic retinopathy (DR) and age-related macular degeneration (AMD) are frequent ocular diseases with potentially sight-threatening outcomes. In the present review we discuss major findings of proteomic studies of RAO, RVO, DR and AMD, including an overview of ocular proteome changes associated with anti-vascular endothelial growth factor (VEGF) treatments. Despite the severe outcomes of RAO, the proteome of the disease remains largely unstudied. There is also limited knowledge about the proteome of RVO, but proteomic studies suggest that RVO is associated with remodeling of the extracellular matrix and adhesion processes. Proteomic studies of DR have resulted in the identification of potential therapeutic targets such as carbonic anhydrase-I. Proliferative diabetic retinopathy is the most intensively studied stage of DR. Proteomic studies have established VEGF, pigment epithelium-derived factor (PEDF) and complement components as key factors associated with AMD. The aim of this review is to highlight the major milestones in proteomics in RAO, RVO, DR and AMD. Through large-scale protein analyses, proteomics is bringing new important insights into these complex pathological conditions.
[Mh] Termos MeSH primário: Retinopatia Diabética/patologia
Degeneração Macular/patologia
Proteoma/análise
Proteômica
Oclusão da Artéria Retiniana/patologia
Oclusão da Veia Retiniana/patologia
[Mh] Termos MeSH secundário: Anticorpos/imunologia
Anticorpos/uso terapêutico
Retinopatia Diabética/tratamento farmacológico
Retinopatia Diabética/metabolismo
Proteínas do Olho/metabolismo
Seres Humanos
Degeneração Macular/tratamento farmacológico
Degeneração Macular/metabolismo
Fatores de Crescimento Neural/metabolismo
Oclusão da Artéria Retiniana/tratamento farmacológico
Oclusão da Artéria Retiniana/metabolismo
Oclusão da Veia Retiniana/tratamento farmacológico
Oclusão da Veia Retiniana/metabolismo
Serpinas/metabolismo
Fator A de Crescimento do Endotélio Vascular/imunologia
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Antibodies); 0 (Eye Proteins); 0 (Nerve Growth Factors); 0 (Proteome); 0 (Serpins); 0 (Vascular Endothelial Growth Factor A); 0 (pigment epithelium-derived factor)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE


  6 / 9957 MEDLINE  
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[PMID]:28465658
[Au] Autor:Godisela KK; Reddy SS; Kumar CU; Saravanan N; Reddy PY; Jablonski MM; Ayyagari R; Reddy GB
[Ad] Endereço:National Institute of Nutrition, Hyderabad, India.
[Ti] Título:Impact of obesity with impaired glucose tolerance on retinal degeneration in a rat model of metabolic syndrome.
[So] Source:Mol Vis;23:263-274, 2017.
[Is] ISSN:1090-0535
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: Metabolic syndrome (MetS) is associated with several degenerative diseases, including retinal degeneration. Previously, we reported on progressive retinal degeneration in a spontaneous obese rat (WNIN/Ob) model. In this study, we investigated the additional effect of impaired glucose tolerance (IGT), an essential component of MetS, on retinal degeneration using the WNIN/GR-Ob rat model. METHODS: The retinal morphology and ultrastructure of WNIN/GR-Ob and age-matched littermate lean rats were studied by microscopy and immunohistochemistry. The retinal transcriptome of WNIN/GR-Ob was compared with the respective lean controls and with the WNIN/Ob model using microarray analysis. Expression of selected retinal marker genes was studied via real-time PCR. RESULTS: Progressive loss of photoreceptor cells was observed in WNIN/GR-Ob rats with an onset as early as 3 months. Similarly, thinning of the inner nuclear layer was observed from 6 months in these rats. Immunohistochemical analysis showed decreased levels of rhodopsin and postsynaptic density protein-95 (PSD-95) proteins and increased levels of glial fibrillary acidic protein (GFAP), vascular endothelial growth factor (VEGF), and calretinin in WNIN/GR-Ob rats compared with the age-matched lean controls, further supporting cellular stress/damage and retinal degeneration. The retinal transcriptome analysis indicated altered expression profiles in both the WNIN/GR-Ob and WNIN/Ob rat models compared to their respective lean controls; these pathways are associated with activation of pathways like cellular oxidative stress response, inflammation, apoptosis, and phototransduction, although the changes were more prominent in WNIN/GR-Ob than in WNIN/Ob animals. CONCLUSIONS: WNIN/GR-Ob rats with added glucose intolerance developed retinal degeneration similar to the parent line WNIN/Ob. The severity of retinal degeneration was greater in WNIN/GR-Ob rats compared to WNIN/Ob, suggesting a possible role for IGT in this model. Hence, the WNIN/GR-Ob model could be a valuable tool for investigating the impact of MetS on retinal degeneration pathology.
[Mh] Termos MeSH primário: Modelos Animais de Doenças
Intolerância à Glucose/complicações
Síndrome Metabólica/etiologia
Obesidade/complicações
Degeneração Retiniana/etiologia
[Mh] Termos MeSH secundário: Animais
Calbindina 2/metabolismo
Proteína 4 Homóloga a Disks-Large/metabolismo
Proteína Glial Fibrilar Ácida/metabolismo
Intolerância à Glucose/metabolismo
Intolerância à Glucose/fisiopatologia
Masculino
Síndrome Metabólica/metabolismo
Síndrome Metabólica/fisiopatologia
Obesidade/metabolismo
Obesidade/fisiopatologia
Células Fotorreceptoras de Vertebrados/patologia
Ratos
Ratos Wistar
Reação em Cadeia da Polimerase em Tempo Real
Degeneração Retiniana/metabolismo
Degeneração Retiniana/fisiopatologia
Rodopsina/metabolismo
Fator A de Crescimento do Endotélio Vascular/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Calb2 protein, rat); 0 (Calbindin 2); 0 (Disks Large Homolog 4 Protein); 0 (Dlg4 protein, rat); 0 (Glial Fibrillary Acidic Protein); 0 (Vascular Endothelial Growth Factor A); 0 (glial fibrillary acid protein, rat); 0 (vascular endothelial growth factor A, rat); 9009-81-8 (Rhodopsin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  7 / 9957 MEDLINE  
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[PMID]:28465655
[Au] Autor:Zhang Y; Cross SD; Stanton JB; Marmorstein AD; Le YZ; Marmorstein LY
[Ad] Endereço:Department of Ophthalmology, Mayo Clinic, Rochester, MN.
[Ti] Título:Early AMD-like defects in the RPE and retinal degeneration in aged mice with RPE-specific deletion of or .
[So] Source:Mol Vis;23:228-241, 2017.
[Is] ISSN:1090-0535
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: To examine the effects of autophagy deficiency induced by RPE-specific deletion of or in mice as a function of age. METHODS: Conditional knockout mice with a floxed allele of or were crossed with inducible transgenic mice. -directed RPE-specific Cre recombinase expression was induced with doxycycline feeding in the resulting mice. Cre-mediated deletion of floxed or resulted in RPE-specific inactivation of the or gene. Plastic and thin retinal sections were analyzed with light and electron microscopy for histological changes. Photoreceptor outer segment (POS) thickness in plastic sections was measured using the Adobe Photoshop CS4 extended ruler tool. Autophagic adaptor p62/SQSTM1 and markers for oxidatively damaged lipids, proteins, and DNA were examined with immunofluorescence staining of cryosections. Fluorescence signals were quantified using Image J software. RESULTS: Accumulation of p62/SQSTM1 reflecting autophagy deficiency was observed in the RPE of the and mice. 3-nitrotyrosine, advanced glycation end products (AGEs), and 8-hydroxy-2'-deoxyguanosine (8-OHdG), markers for oxidatively damaged proteins and DNA, were also found to accumulate in the RPE of these mice. We observed retinal degeneration in 35% of the mice and 45% of the mice at 8 to 24 months old. Degeneration severity and the number of mice with degeneration increased with age. The mean POS thickness of these mice was 25 µm at 8-12 months, 15 µm at 13-18 months, and 3 µm at 19-24 months, compared to 35 µm, 30 µm, and 24 µm in the wild-type mice, respectively. Early age-related macular degeneration (AMD)-like RPE defects were found in all the and mice 13 months old or older, including vacuoles, uneven RPE thickness, diminished basal infoldings, RPE hypertrophy/hypotrophy, pigmentary irregularities, and necrosis. The severity of the RPE defects increased with age and in the mice with retinal degeneration. RPE atrophy and choroidal neovascularization (CNV) were occasionally observed in the and mice with advanced age. CONCLUSIONS: Autophagy deficiency induced by RPE-specific deletion of or predisposes but does not necessarily drive the development of AMD-like phenotypes or retinal degeneration.
[Mh] Termos MeSH primário: Proteína 5 Relacionada à Autofagia/genética
Proteína 7 Relacionada à Autofagia/genética
Autofagia
Deleção de Genes
Degeneração Macular/genética
Degeneração Retiniana/genética
Epitélio Pigmentado da Retina/patologia
[Mh] Termos MeSH secundário: Alelos
Animais
Biomarcadores/metabolismo
Eletrorretinografia
Feminino
Técnica Indireta de Fluorescência para Anticorpo
Degeneração Macular/patologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Camundongos Knockout
Camundongos Transgênicos
Degeneração Retiniana/patologia
Epitélio Pigmentado da Retina/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Atg5 protein, mouse); 0 (Atg7 protein, mouse); 0 (Autophagy-Related Protein 5); 0 (Biomarkers); EC 6.2.1.45 (Autophagy-Related Protein 7)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE


  8 / 9957 MEDLINE  
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[PMID]:28458506
[Au] Autor:Qi S; Wang C; Song D; Song Y; Dunaief JL
[Ad] Endereço:Department of Ophthalmology, The Second Hospital of Jilin University, Jilin, China.
[Ti] Título:Intraperitoneal injection of (-)-Epigallocatechin-3-gallate protects against light-induced photoreceptor degeneration in the mouse retina.
[So] Source:Mol Vis;23:171-178, 2017.
[Is] ISSN:1090-0535
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PURPOSE: (-)-epigallocatechin-3-gallate (EGCG), a major catechin component of green tea, is reported to delay or prevent certain forms of cancer, arthritis, cardiovascular disease, and neurodegenerative disorders. In this study, we determined if systemically administered EGCG could protect the retina against light damage (LD) in mice. METHODS: BALB/cJ mice were treated with either EGCG or saline via intraperitoneal (IP) injection, and then placed under constant cool white light-emitting diode (LED) light (10,000 lux) for 5 h. Retinal structure and function were evaluated using optical coherence tomography (OCT), histology, and electroretinography (ERG) 7 days after LD. In addition, the mRNAs of several oxidative stress genes were quantified by qPCR before LD and 24 h after LD. RESULTS: OCT and photomicrographs of mouse retinas showed morphologic protection of photoreceptors. Mice in the EGCG group had significantly higher ERG amplitudes for all three wave types compared with mice in the saline control group, which indicated that EGCG protected retinal function. Furthermore, qPCR results showed that EGCG administration can increase the mRNA level of the antioxidant gene before LD and 24 h after LD. CONCLUSIONS: The IP injection of EGCG attenuated the detrimental effects of bright light on the retinas of BALB/cJ mice by protecting the structure and function of the retina.
[Mh] Termos MeSH primário: Antioxidantes/uso terapêutico
Catequina/análogos & derivados
Luz/efeitos adversos
Células Fotorreceptoras de Vertebrados/efeitos da radiação
Lesões Experimentais por Radiação/prevenção & controle
Degeneração Retiniana/prevenção & controle
[Mh] Termos MeSH secundário: Animais
Antioxidantes/administração & dosagem
Catequina/administração & dosagem
Catequina/uso terapêutico
Eletrorretinografia
Injeções Intraperitoneais
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Estresse Oxidativo/genética
RNA Mensageiro/genética
Lesões Experimentais por Radiação/etiologia
Lesões Experimentais por Radiação/fisiopatologia
Reação em Cadeia da Polimerase em Tempo Real
Retina/fisiopatologia
Degeneração Retiniana/etiologia
Degeneração Retiniana/fisiopatologia
Superóxido Dismutase/genética
Tomografia de Coerência Óptica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (RNA, Messenger); 8R1V1STN48 (Catechin); BQM438CTEL (epigallocatechin gallate); EC 1.15.1.1 (Superoxide Dismutase); EC 1.15.1.1 (superoxide dismutase 2)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  9 / 9957 MEDLINE  
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[PMID]:27774623
[Au] Autor:Liu HH; He Z; Nguyen CT; Vingrys AJ; Bui BV
[Ad] Endereço:Department of Optometry & Vision Sciences, University of Melbourne, Parkville, Australia.
[Ti] Título:Reversal of functional loss in a rat model of chronic intraocular pressure elevation.
[So] Source:Ophthalmic Physiol Opt;37(1):71-81, 2017 01.
[Is] ISSN:1475-1313
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:PURPOSE: This pilot study considered whether intraocular pressure (IOP) lowering could reverse ganglion cell dysfunction in a rat model of chronic ocular hypertension. METHODS: A circumlimbal suture was applied in one eye to induce ocular hypertension (n = 7) in Long-Evans rats. The contralateral eye served as an untreated control. After 8 weeks of IOP elevation the suture was removed to lower IOP for the remaining 7 weeks. Electroretinogram (ERG) and optical coherence tomography (OCT) were measured at baseline, 2, 4, 8, 12 and 15 weeks. Retinae were collected for histology at week 15. RESULTS: In sutured eyes, IOP was elevated by 7-11 mmHg above control eyes (12 ± 0.2 mmHg [standard error of the mean]). Eight weeks of chronic IOP elevation resulted in a reduction of the ganglion cell mediated positive Scotopic Threshold Response (pSTR, -25 ± 7% of baseline), as well as smaller photoreceptor (-7 ± 4%) and bipolar cell mediated responses (-6 ± 5%). After suture removal, IOP recovered to normal. By 15 weeks the a-wave (0 ± 6%), b-wave (-2 ± 6%) and pSTR had recovered back to baseline (from -25 ± 7% to -4 ± 6%). The retinal nerve fiber layer was thinned by -9 ± 3% at week 8 and showed no further decline at week 15 (-10 ± 2%). Cell numbers in the ganglion cell layer were similar between suture removal and control eyes at week 15 (3543 ± 478 vs 4057 ± 476 cells mm ). CONCLUSIONS: The circumlimbal suture model might be a useful platform to study the reversibility of neuronal dysfunction from chronic IOP challenge.
[Mh] Termos MeSH primário: Pressão Intraocular/fisiologia
Hipertensão Ocular/fisiopatologia
Degeneração Retiniana/etiologia
Células Ganglionares da Retina/patologia
[Mh] Termos MeSH secundário: Animais
Doença Crônica
Modelos Animais de Doenças
Eletrorretinografia
Masculino
Hipertensão Ocular/complicações
Projetos Piloto
Ratos
Ratos Long-Evans
Degeneração Retiniana/diagnóstico
Degeneração Retiniana/fisiopatologia
Tomografia de Coerência Óptica
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1707
[Cu] Atualização por classe:180109
[Lr] Data última revisão:
180109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE
[do] DOI:10.1111/opo.12331


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[PMID]:29260193
[Au] Autor:Srinivasan S; Dehghani C; Pritchard N; Edwards K; Russell AW; Malik RA; Efron N
[Ad] Endereço:Institute of Health and Biomedical Innovation, Queensland University of Technology, Brisbane, Queensland, Australia.
[Ti] Título:Corneal and Retinal Neuronal Degeneration in Early Stages of Diabetic Retinopathy.
[So] Source:Invest Ophthalmol Vis Sci;58(14):6365-6373, 2017 Dec 01.
[Is] ISSN:1552-5783
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Purpose: To examine the neuronal structural integrity of cornea and retina as markers for neuronal degeneration in nonproliferative diabetic retinopathy (NPDR). Methods: Participants were recruited from the broader Brisbane community, Queensland, Australia. Two hundred forty-one participants (187 with diabetes and 54 nondiabetic controls) were examined. Diabetic retinopathy (DR) was graded according to the Early Treatment Diabetic Retinopathy Study (ETDRS) scale. Corneal nerve fiber length (CNFL), corneal nerve branch density (CNBD), corneal nerve fiber tortuosity (CNFT), full retinal thickness, retinal nerve fiber layer (RNFL), ganglion cell complex (GCC), focal (FLV) and global loss volumes (GLV), hemoglobin A1c (HbA1c), nephropathy, neuropathy, and cardiovascular measures were examined. Results: The central zone (P = 0.174), parafoveal thickness (P = 0.090), perifovea (P = 0.592), RNFL (P = 0.866), GCC (P = 0.798), and GCC GLV (P = 0.338) did not differ significantly between the groups. In comparison to the control group, those with very mild NPDR and those with mild NPDR had significantly higher focal loss in GCC volume (P = 0.036). CNFL was significantly lower in those with mild NPDR (P = 0.004) in comparison to the control group and those with no DR. The CNBD (P = 0.094) and CNFT (P = 0.458) did not differ between the groups. Conclusions: Both corneal and retinal neuronal degeneration may occur in early stages of diabetic retinopathy. Further studies are required to examine these potential markers for neuronal degeneration in the absence of clinical signs of DR.
[Mh] Termos MeSH primário: Córnea/inervação
Retinopatia Diabética/patologia
Doenças do Sistema Nervoso Periférico/patologia
Retina/patologia
Degeneração Retiniana/patologia
[Mh] Termos MeSH secundário: Adolescente
Adulto
Idoso
Análise de Variância
Estudos de Casos e Controles
Feminino
Seres Humanos
Masculino
Meia-Idade
Fibras Nervosas/patologia
Queensland
Células Ganglionares da Retina
Tomografia de Coerência Óptica
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171227
[Lr] Data última revisão:
171227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1167/iovs.17-22736



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