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  1 / 1674 MEDLINE  
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[PMID]:29523717
[Au] Autor:Guo J; Wang S; Yu X; Dong R; Li Y; Mei X; Shen Y
[Ad] Endereço:Beijing University of Agriculture CITY: Beijing China [CN].
[Ti] Título:Polyamines Regulate Strawberry Fruit Ripening by ABA, IAA, and Ethylene.
[So] Source:Plant Physiol;, 2018 Mar 09.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polyamines (PAs) participate in many plant growth and developmental processes, including fruit ripening. However, it is not clear whether PAs play a role in the ripening of strawberry (Fragaria ananassa), a model non-climacteric plant. Here, we found that the content of the PA spermine (Spm) increased more sharply after the onset of fruit coloration than did that of the PAs putrescine (Put) or spermidine (Spd). Spm dominance in ripe fruit resulted from abundant transcripts of a strawberry S-adenosyl-L-methionine decarboxylase gene (FaSAMDC), which encodes an enzyme that generates a residue needed for PA biosynthesis. Exogenous Spm and Spd promoted fruit coloration, while exogenous Put and a SAMDC inhibitor inhibited coloration. Based on transcriptome data, up- and downregulation of FaSAMDC expression promoted and inhibited ripening, respectively, which coincided with changes in several physiological parameters and their corresponding gene transcripts, including firmness, anthocyanin content, sugar content, polyamine content, auxin (IAA) content, abscisic acid (ABA) content, and ethylene emission. Using isothermal titration calorimetry, we found that FaSAMDC also had a high enzymatic activity with a Kd of 1.7 × 10-3 M. In conclusion, PAs, especially Spm, regulate strawberry fruit ripening in an ABA-dominated, IAA-participating and ethylene-coordinated manner, and FaSAMDC plays an important role in ripening.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180310
[Lr] Data última revisão:
180310
[St] Status:Publisher


  2 / 1674 MEDLINE  
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[PMID]:29458464
[Au] Autor:Melo LA; Ventura JA; Costa H; Kitajima EW; Ferreira J; Bedendo IP
[Ad] Endereço:1​Departamento de Fitopatologia e Nematologia, ESALQ, Universidade de São Paulo, Caixa Postal 09, 13418-900 Piracicaba, SP, Brazil.
[Ti] Título:Delineation of a novel subgroup 16SrXIII-J phytoplasma, a 'Candidatus Phytoplasma hispanicum'-related strain, based on computer-simulated RFLP and phylogenetic analysis.
[So] Source:Int J Syst Evol Microbiol;68(3):962-966, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Symptoms of fruit phyllody and slow growth, which are suggestive of phytoplasma infection, were observed in strawberry plants cultivated in commercial fields. In order to provide evidence of association of phytoplasma with affected plants, assays for detecting and identifying were performed through computer-simulated restriction fragment length polymorphism (RFLP) and phylogenetic analysis. Total DNA was extracted from symptomatic and asymptomatic samples and used as template in nested PCR primed by the primers P1/Tint followed by R16F2n/16R2. Amplified DNA fragments of 1.2 kb from the 16S rRNA gene revealed the presence of phytoplasma in all symptomatic samples. Molecular detection was confirmed by electron transmission microscopy, which evidenced pleomorphic bodies in the phloem vessels. Nucleotide sequence representative of the strawberry phytoplasma shared 97.2 to 99 % similarity with phytoplasmas currently classified as members of the distinct subgroups within the 16SrXIII group. Similarity coefficient (F) values ranged from 0.70 to 0.92, indicating that strawberry phytoplasma delineates a new strain in addition to 'Candidatus Phytoplasma hispanicum'-related strains. The evolutionary tree displayed that this strain emerges as a new branch in relation to those previously described. The novel strain, designated SFP (strawberry fruit phyllody) phytoplasma represents the new 16SrXIII-J subgroup and its sequence, denominated SFP-Br02, was deposited in the GenBank database (EU719108). These findings contribute for the knowledge of the genetic diversity existing among members of the group 16SrXIII and establishes strawberry as an additional host of representatives of this group in Brazil.
[Mh] Termos MeSH primário: Fragaria/microbiologia
Filogenia
Phytoplasma/classificação
Doenças das Plantas/microbiologia
Polimorfismo de Fragmento de Restrição
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Brasil
Primers do DNA
DNA Bacteriano/genética
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002547


  3 / 1674 MEDLINE  
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[PMID]:29253147
[Au] Autor:Edger PP; VanBuren R; Colle M; Poorten TJ; Wai CM; Niederhuth CE; Alger EI; Ou S; Acharya CB; Wang J; Callow P; McKain MR; Shi J; Collier C; Xiong Z; Mower JP; Slovin JP; Hytönen T; Jiang N; Childs KL; Knapp SJ
[Ad] Endereço:Department of Horticulture, Ecology, Evolutionary Biology, and Behavior, Department of Plant Biology, and Center for Genomics Enabled Plant Science, Michigan State University, East Lansing, Michigan, 48823.
[Ti] Título:Single-molecule sequencing and optical mapping yields an improved genome of woodland strawberry (Fragaria vesca) with chromosome-scale contiguity.
[So] Source:Gigascience;7(2):1-7, 2018 Feb 01.
[Is] ISSN:2047-217X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Background: Although draft genomes are available for most agronomically important plant species, the majority are incomplete, highly fragmented, and often riddled with assembly and scaffolding errors. These assembly issues hinder advances in tool development for functional genomics and systems biology. Findings: Here we utilized a robust, cost-effective approach to produce high-quality reference genomes. We report a near-complete genome of diploid woodland strawberry (Fragaria vesca) using single-molecule real-time sequencing from Pacific Biosciences (PacBio). This assembly has a contig N50 length of ∼7.9 million base pairs (Mb), representing a ∼300-fold improvement of the previous version. The vast majority (>99.8%) of the assembly was anchored to 7 pseudomolecules using 2 sets of optical maps from Bionano Genomics. We obtained ∼24.96 Mb of sequence not present in the previous version of the F. vesca genome and produced an improved annotation that includes 1496 new genes. Comparative syntenic analyses uncovered numerous, large-scale scaffolding errors present in each chromosome in the previously published version of the F. vesca genome. Conclusions: Our results highlight the need to improve existing short-read based reference genomes. Furthermore, we demonstrate how genome quality impacts commonly used analyses for addressing both fundamental and applied biological questions.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[St] Status:In-Data-Review
[do] DOI:10.1093/gigascience/gix124


  4 / 1674 MEDLINE  
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[PMID]:29451893
[Au] Autor:Fan R; Cockerton HM; Armitage AD; Bates H; Cascant-Lopez E; Antanaviciute L; Xu X; Hu X; Harrison RJ
[Ad] Endereço:NIAB-EMR, East Malling, Kent, United Kingdom.
[Ti] Título:Vegetative compatibility groups partition variation in the virulence of Verticillium dahliae on strawberry.
[So] Source:PLoS One;13(2):e0191824, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Verticillium dahliae infection of strawberry (Fragaria x ananassa) is a major cause of disease-induced wilting in soil-grown strawberries across the world. To understand what components of the pathogen are affecting disease expression, the presence of the known effector VdAve1 was screened in a sample of Verticillium dahliae isolates. Isolates from strawberry were found to contain VdAve1 and were divided into two major clades, based upon their vegetative compatibility groups (VCG); no UK strawberry isolates contained VdAve1. VC clade was strongly related to their virulence levels. VdAve1-containing isolates pathogenic on strawberry were found in both clades, in contrast to some recently published findings. On strawberry, VdAve1-containing isolates had significantly higher virulence during early infection, which diminished in significance as the infection progressed. Transformation of a virulent non-VdAve1 containing isolate, with VdAve1 was found neither to increase nor decrease virulence when inoculated on a susceptible strawberry cultivar. There are therefore virulence factors that are epistatic to VdAve1 and potentially multiple independent routes to high virulence on strawberry in V. dahliae lineages. Genome sequencing a subset of isolates across the two VCGs revealed that isolates were differentiated at the whole genome level and contained multiple changes in putative effector content, indicating that different clonal VCGs may have evolved different strategies for infecting strawberry, leading to different virulence levels in pathogenicity tests. It is therefore important to consider both clonal lineage and effector complement as the adaptive potential of each lineage will differ, even if they contain the same race determining effector.
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180305
[Lr] Data última revisão:
180305
[St] Status:In-Process
[do] DOI:10.1371/journal.pone.0191824


  5 / 1674 MEDLINE  
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[PMID]:29495391
[Au] Autor:Hossain MR; Kim HT; Shanmugam A; Nath UK; Goswami G; Song JY; Park JI; Nou IS
[Ad] Endereço:Department of Horticulture, Suncheon National University, 255 Jungang-ro, Suncheon, Jeonnam 57922, Korea. m.r.hossain@bau.edu.bd.
[Ti] Título:Expression Profiling of Regulatory and Biosynthetic Genes in Contrastingly Anthocyanin Rich Strawberry (Fragaria × ananassa) Cultivars Reveals Key Genetic Determinants of Fruit Color.
[So] Source:Int J Mol Sci;19(3), 2018 Feb 26.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Anthocyanins are the resultant end-point metabolites of phenylapropanoid/flavonoid (F/P) pathway which is regulated at transcriptional level via a series of structural genes. Identifying the key genes and their potential interactions can provide us with the clue for novel points of intervention for improvement of the trait in strawberry. We profiled the expressions of putative regulatory and biosynthetic genes of cultivated strawberry in three developmental and characteristically colored stages of fruits of contrastingly anthocyanin rich cultivars: Tokun, Maehyang and Soelhyang. Besides a well-characterized positive regulator, , and might also act as potential positive regulators, while , , and as potential negative regulators of anthocyanin biosynthesis in these high-anthocyanin cultivars. Among the early BGs, , , , , and and among the late BGs, , , and showed significantly higher expression in ripe fruits of high anthocyanin cultivars Maehyang and Soelhyang. Multivariate analysis revealed the association of these genes with total anthocyanins. Increasingly higher expressions of the key genes along the pathway indicates the progressive intensification of pathway flux leading to final higher accumulation of anthocyanins. Identification of these key genetic determinants of anthocyanin regulation and biosynthesis in Korean cultivars will be helpful in designing crop improvement programs.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180302
[Lr] Data última revisão:
180302
[St] Status:In-Process


  6 / 1674 MEDLINE  
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[PMID]:29262412
[Au] Autor:Qu M; Li K; Han Y; Chen L; Li Z; Han Y
[Ad] Endereço:Jiangsu Key Laboratory of Phylogenomics and Comparative Genomics, School of Life Sciences, Jiangsu Normal University, Xuzhou, China.
[Ti] Título:Integrated Karyotyping of Woodland Strawberry (Fragaria vesca) with Oligopaint FISH Probes.
[So] Source:Cytogenet Genome Res;153(3):158-164, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Chromosome identification is critical for many aspects of cytogenetic research. However, for Fragaria vesca, definite identification of individual chromosomes is almost impossible because of their small size and high similarity. Here, we demonstrate that bulked oligonucleotide (oligo) probes can be used as chromosome-specific DNA markers for chromosome identification in F. vesca. Oligos specific to entire pseudochromosomes in the draft genome of F. vesca were identified and synthesized as libraries. In all, we synthesized 6 oligo libraries corresponding to 6 pseudochromosomes of F. vesca. These libraries were amplified and labeled as probes for fluorescence in situ hybridization (FISH). Two rounds of multicolor FISH analysis were sequentially conducted on the same metaphase cells with each round including 3 probe libraries, which permitted simultaneous identification of all chromosomes of F. vesca. Moreover, 45S and 5S rDNA were mapped to chromosomes 1, 2, and 7, respectively. A karyotype of metaphase chromosomes was constructed, representing the first FISH-based molecular cytogenetic karyotype of F. vesca. Our study can serve as a basis for future comparative cytogenetic research through cross-species chromosome painting using bulked oligo probes and will facilitate the application of breeding technologies that rely on the identification of chromosomes in the genus Fragaria.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:180228
[Lr] Data última revisão:
180228
[St] Status:In-Process
[do] DOI:10.1159/000485283


  7 / 1674 MEDLINE  
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[PMID]:29477905
[Au] Autor:Roy S; Wu B; Liu W; Archbold DD
[Ad] Endereço:Department of Horticulture, University of Kentucky, Lexington, KY 40546, United States. Electronic address: sutaparoy09@gmail.com.
[Ti] Título:Comparative analyses of polyphenolic composition of Fragaria spp. color mutants.
[So] Source:Plant Physiol Biochem;125:255-261, 2018 Feb 13.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:White-fruited mutants of Fragaria vesca, and one of F. x ananassa, were studied to determine the identity and quantity of major flavonols (FVLs), flavan-3-ols (FV3Ls), hydroxycinnamic acids (HCAs), and ellagic acid (EA)-derived compounds, by using HPLC-MS. The content of 22 compounds across the major groups were used to assess the possibility of unique mutations among the mutant gentoypes. Total HCAs were lower in the white than the red cultivars of both species, except for 2 white F. vesca cultivars. Total FVLs were comparable in white fruit of both species, although a red F. x ananassa had more than a red F. vesca. Total FV3Ls were higher in red than white cultivars of both species. Total EA-derived content was generally higher in white than in red F. vesca. Principal component analysis and a combined heatmap and hierarchical cluster analysis clearly discriminated among the five white F. vesca genotypes.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180225
[Lr] Data última revisão:
180225
[St] Status:Publisher


  8 / 1674 MEDLINE  
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[PMID]:29177844
[Au] Autor:Ishibashi M; Nabe T; Nitta Y; Tsuruta H; Iduhara M; Uno Y
[Ad] Endereço:Department of Plant Resource Science, Graduate School of Agricultural Science, Kobe University, Rokko, Kobe, Hyogo, 657-8501, Japan.
[Ti] Título:Analysis of major paralogs encoding the Fra a 1 allergen based on their organ-specificity in Fragaria × ananassa.
[So] Source:Plant Cell Rep;37(3):411-424, 2018 Mar.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: Fra a 1 protein in strawberry causes oral allergic syndrome. Over 39 Fra a 1 paralogs have been identified in strawberry genome. Fra a 1.01 is major accumulating protein in edible organs. Strawberry fruits contain allergenic proteins that cause oral allergic syndrome. The hypothesized major allergen is Fra a 1, an ortholog of the birch pollen allergen protein Bet v 1. We organized Fra a 1 genes and analyzed their localizations at the transcriptional and translational levels. In total, 15 new Fra a 1 proteins were identified from the genomic database, increasing the total number of Fra a 1 to 30 proteins encoded by 39 genes. Fra a 1.02 was mostly expressed in receptacles, and Fra a 1.01 in achenes, when analyzed by RNA sequencing. Immunoblotting showed that the Fra a 1.01 protein was broadly accumulated in strawberry organs, while the Fra a 1.02 protein was mostly expressed in receptacles. Recombinant Fra a 1.01 strongly reacted with human IgE. The mRNA and protein expression levels of Fra a 1 did not correlate, indicating the importance of protein levels when evaluating the abundance of allergens in strawberry. Based on the localizations, accumulation levels and reactivity to human IgE, we determined that Fra a 1.01 was the most important allergen, followed by Fra a 1.02, and then other Fra a 1 proteins. The information obtained here will be useful for selecting the target Fra a 1 paralogs when breeding hypoallergenic strawberry.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:180225
[Lr] Data última revisão:
180225
[St] Status:In-Process
[do] DOI:10.1007/s00299-017-2237-6


  9 / 1674 MEDLINE  
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[PMID]:29179062
[Au] Autor:Xu Y; Hu D; Li Y; Sun C; Chen W
[Ad] Endereço:Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang Univers
[Ti] Título:An effective method for preparation of high-purity pelargonidin-3-O-glucoside from strawberry and its protective effect on cellular oxidative stress.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:211-220, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence indicates that consumption of berries may exert beneficial effects against oxidative stress mediated diseases. Pelargonidin-3-O-glucoside (Pg3G), a bioactive ingredient in strawberry, has been reported to possess a potent antioxidant capacity. This study was therefore designed to develop an effective method to prepare pure Pg3G from strawberry and investigate its protective effect against H O -induced oxidative stress. According to our results, Pg3G occupied 85.55% of total anthocyanin content in strawberry. 240mg of Pg3G with the purity of 97.26% was finally isolated from 320g of strawberry lyophilized powder (SLP) by combination of AB-8 macroporous resin and high-speed counter-current chromatography (HSCCC) technologies. Further study unveiled that Pg3G significantly inhibited H O -induced ROS generation, GSH depletion and mitochondrial dysfunction, thereby ameliorating H O -induced oxidative stress. Overall, this study suggests that pelargonidin-3-O-glucoside can be used as a natural bioactive agent to prevent cellular oxidative stress.
[Mh] Termos MeSH primário: Antocianinas/isolamento & purificação
Antocianinas/farmacologia
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Fragaria/química
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antocianinas/análise
Antocianinas/química
Antioxidantes/análise
Antioxidantes/química
Sobrevivência Celular/efeitos dos fármacos
Distribuição Contracorrente/métodos
Glutationa/metabolismo
Células Hep G2
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 8H1WZY9R6P (pelargonidin-3-glucoside); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  10 / 1674 MEDLINE  
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[PMID]:29350442
[Au] Autor:Elías JM; Guerrero-Molina MF; Martínez-Zamora MG; Díaz-Ricci JC; Pedraza RO
[Ad] Endereço:Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Tucumán, Argentina.
[Ti] Título:Role of ethylene and related gene expression in the interaction between strawberry plants and the plant growth-promoting bacterium Azospirillum brasilense.
[So] Source:Plant Biol (Stuttg);, 2018 Jan 19.
[Is] ISSN:1438-8677
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Induced systemic resistance (ISR) is one of the indirect mechanisms of growth promotion exerted by plant growth-promoting bacteria, and can be mediated by ethylene (ET). We assessed ET production and the expression of related genes in the Azospirillum-strawberry plant interaction. Ethylene production was evaluated by gas chromatography in plants inoculated or not with A. brasilense REC3. Also, plants were treated with AgNO , an inhibitor of ET biosynthesis; with 1-aminocyclopropane-1-carboxylic acid (ACC), a precursor of ET biosynthesis; and with indole acetic acid (IAA). Plant dry biomass and the growth index were determined to assess the growth-promoting effect of A. brasilense REC3 in strawberry plants. Quantitative real time PCR (qRT-PCR) was performed to analyse relative expression of the genes Faetr1, Faers1 and Faein4, which encode ET receptors; Factr1 and Faein2, involved in the ET signalling pathway; Faacs1 encoding ACC synthase; Faaco1 encoding ACC oxidase; and Faaux1 and Faami1 for IAA synthesis enzymes. Results showed that ET acts as a rapid and transient signal in the first 12 h post-treatment. A. brasilense REC3-inoculated plants had a significantly higher growth index compared to control plants. Modulation of the genes Faetr1, Faers1, Faein4, Factr1, Faein2 and Faaco1 indicated activation of ET synthesis and signalling pathways. The up-regulation of Faaux1 and Faami1 involved in IAA synthesis suggested that inoculation with A. brasilense REC3 induces production of this auxin, modulating ET signalling. Ethylene production and up-regulation of genes associated with ET signalling in strawberry plants inoculated with A. brasilense REC3 support the priming activation characteristic of ISR. This type of resistance and the activation of systemic acquired resistance previously observed in this interaction indicate that both are present in strawberry plants, could act synergistically and increase protection against pathogens.
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180218
[Lr] Data última revisão:
180218
[St] Status:Publisher
[do] DOI:10.1111/plb.12697



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