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  1 / 6863 MEDLINE  
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[PMID]:28457886
[Au] Autor:Guo A; Huang S; Yu J; Wang H; Li H; Pei G; Shen L
[Ad] Endereço:State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
[Ti] Título:Single-Cell Dynamic Analysis of Mitosis in Haploid Embryonic Stem Cells Shows the Prolonged Metaphase and Its Association with Self-diploidization.
[So] Source:Stem Cell Reports;8(5):1124-1134, 2017 May 09.
[Is] ISSN:2213-6711
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The recent establishment of mammalian haploid embryonic stem cells (ESCs) provides new possibilities for genetic screening and for understanding genome evolution and function. However, the dynamics of mitosis in haploid ESCs is still unclear. Here, we report that the duration of mitosis in haploid ESCs, especially the metaphase, is significantly longer than that in diploid ESCs. Delaying mitosis by chemicals increased self-diploidization of haploid ESCs, while shortening mitosis stabilized haploid ESCs. Taken together, our study suggests that the delayed mitosis of haploid ESCs is associated with self-diploidization.
[Mh] Termos MeSH primário: Células-Tronco Embrionárias/citologia
Haploidia
Metáfase
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Diploide
Camundongos
Análise de Célula Única
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180222
[Lr] Data última revisão:
180222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


  2 / 6863 MEDLINE  
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[PMID]:29372794
[Au] Autor:Safronova LD; Kupriyanova LA
[Ti] Título:[Metaphase and meiotic chromosomes, synaptonemal complexes (SC) of the lizard Zootoca vivipara].
[So] Source:Genetika;52(11):1311-7, 2016 Nov.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Somatic mitotic and meiotic chromosomes at the pachytene and at the metaphase I of the males of the viviparous lizard, Zootoca vivipara (Lichtenstein, 1823), from northwestern Russia, belonging to the Russian form of Z. v. vivipara, are examined. The spreading of synaptonemal complexes (SC) of their chromosomes are obtained and analyzed for the first time. Eighteen SC are observed, including SC of the Z1Z1 (pairs 5 or 6) and the Z2Z2 (pair 13) sex chromosomes. Characteristics of SC are compared with the number and the shape of bivalents and with those of the karyotype structure. In the studied Russian form of Z. v. vivipara, the length ratios of bivalents correlate with that of mitotic chromosomes (2n = 36); however, some specificity in the morphology of SC of the Z1Z1 sex chromosomes is reported in this article.
[Mh] Termos MeSH primário: Lagartos/metabolismo
Metáfase/fisiologia
Espermatócitos/metabolismo
Complexo Sinaptonêmico/metabolismo
[Mh] Termos MeSH secundário: Animais
Masculino
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


  3 / 6863 MEDLINE  
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[PMID]:28740117
[Au] Autor:Glover TW; Wilson TE; Arlt MF
[Ad] Endereço:Department of Human Genetics; the Department of Pathology; and the Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.
[Ti] Título:Fragile sites in cancer: more than meets the eye.
[So] Source:Nat Rev Cancer;17(8):489-501, 2017 07 25.
[Is] ISSN:1474-1768
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Ever since initial suggestions that instability at common fragile sites (CFSs) could be responsible for chromosome rearrangements in cancers, CFSs and associated genes have been the subject of numerous studies, leading to questions and controversies about their role and importance in cancer. It is now clear that CFSs are not frequently involved in translocations or other cancer-associated recurrent gross chromosome rearrangements. However, recent studies have provided new insights into the mechanisms of CFS instability, their effect on genome instability, and their role in generating focal copy number alterations that affect the genomic landscape of many cancers.
[Mh] Termos MeSH primário: Instabilidade Cromossômica
Sítios Frágeis do Cromossomo
Variações do Número de Cópias de DNA
Neoplasias/genética
Oncogenes/genética
[Mh] Termos MeSH secundário: Anáfase
Animais
Quebra Cromossômica
Quebras de DNA de Cadeia Dupla
Replicação do DNA
Rearranjo Gênico
Seres Humanos
Metáfase
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Em] Mês de entrada:1709
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170726
[St] Status:MEDLINE
[do] DOI:10.1038/nrc.2017.52


  4 / 6863 MEDLINE  
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[PMID]:29017027
[Au] Autor:Vukusic K; Buda R; Bosilj A; Milas A; Pavin N; Tolic IM
[Ad] Endereço:Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka cesta 54, 10000 Zagreb, Croatia.
[Ti] Título:Microtubule Sliding within the Bridging Fiber Pushes Kinetochore Fibers Apart to Segregate Chromosomes.
[So] Source:Dev Cell;43(1):11-23.e6, 2017 Oct 09.
[Is] ISSN:1878-1551
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:During cell division, mitotic spindle microtubules segregate chromosomes by exerting forces on kinetochores. What forces drive chromosome segregation in anaphase remains a central question. The current model for anaphase in human cells includes shortening of kinetochore fibers and separation of spindle poles. Both processes require kinetochores to be linked with the poles. Here we show, by combining laser ablation, photoactivation, and theoretical modeling, that kinetochores can separate without any attachment to one spindle pole. This separation requires the bridging fiber, a microtubule bundle that connects sister kinetochore fibers. Bridging fiber microtubules in intact spindles slide apart with kinetochore fibers, indicating strong crosslinks between them. We conclude that sliding of microtubules within the bridging fibers drives pole separation and pushes kinetochore fibers poleward by the friction of passive crosslinks between these fibers. Thus, sliding within the bridging fiber works together with the shortening of kinetochore fibers to segregate chromosomes.
[Mh] Termos MeSH primário: Anáfase/fisiologia
Segregação de Cromossomos/fisiologia
Cinetocoros/metabolismo
Metáfase/fisiologia
Microtúbulos/metabolismo
[Mh] Termos MeSH secundário: Células Cultivadas
Seres Humanos
Modelos Biológicos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171011
[St] Status:MEDLINE


  5 / 6863 MEDLINE  
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[PMID]:28935703
[Au] Autor:Li X; Liu X; Gao M; Han L; Qiu D; Wang H; Xiong B; Sun SC; Liu H; Gu L
[Ad] Endereço:College of Animal Science & Technology, Nanjing Agricultural University, 210095 Nanjing, China.
[Ti] Título:HDAC3 promotes meiotic apparatus assembly in mouse oocytes by modulating tubulin acetylation.
[So] Source:Development;144(20):3789-3797, 2017 10 15.
[Is] ISSN:1477-9129
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Histone deacetylases (HDACs) have been shown to deacetylate numerous cellular substrates that govern a wide array of biological processes. HDAC3, a member of the Class I HDACs, is a highly conserved and ubiquitously expressed protein. However, its roles in meiotic oocytes are not known. In the present study, we find that mouse oocytes depleted of HDAC3 are unable to completely progress through meiosis, and are blocked at metaphase I. These HDAC3 knockdown oocytes show spindle/chromosome organization failure, with severely impaired kinetochore-microtubule attachments. Consistent with this, the level of BubR1, a central component of the spindle assembly checkpoint, at kinetochores is dramatically increased in metaphase oocytes following HDAC3 depletion. Knockdown and overexpression experiments reveal that HDAC3 modulates the acetylation status of α-tubulin in mouse oocytes. Importantly, the deacetylation mimetic mutant tubulin-K40R can partly rescue the defective phenotypes of HDAC3 knockdown oocytes. Our data support a model whereby HDAC3, through deacetylating tubulin, promotes microtubule stability and the establishment of kinetochore-microtubule interaction, consequently ensuring proper spindle morphology, accurate chromosome movement and orderly meiotic progression during oocyte maturation.
[Mh] Termos MeSH primário: Histona Desacetilases/metabolismo
Meiose
Oócitos/metabolismo
Tubulina (Proteína)/metabolismo
[Mh] Termos MeSH secundário: Acetilação
Aneuploidia
Animais
Proteínas de Ciclo Celular/metabolismo
Feminino
Histona Desacetilases/genética
Cinetocoros
Metáfase
Camundongos
Camundongos Endogâmicos ICR
Microtúbulos/metabolismo
Oócitos/citologia
Fenótipo
Proteínas Serina-Treonina Quinases/metabolismo
Fuso Acromático
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bub1b protein, mouse); 0 (Cell Cycle Proteins); 0 (Tubulin); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 3.5.1.98 (Histone Deacetylases); EC 3.5.1.98 (histone deacetylase 3)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170923
[St] Status:MEDLINE
[do] DOI:10.1242/dev.153353


  6 / 6863 MEDLINE  
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[PMID]:28810257
[Au] Autor:Wang GX; He QY; Macas J; Novák P; Neumann P; Meng DX; Zhao H; Guo N; Han S; Zong M; Jin WW; Liu F
[Ad] Endereço:Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (North China), Ministry of Agriculture, Beijing Key Laboratory of Vegetable Germplasm Improvement, Beijing, China.
[Ti] Título:Karyotypes and Distribution of Tandem Repeat Sequences in Brassica nigra Determined by Fluorescence in situ Hybridization.
[So] Source:Cytogenet Genome Res;152(3):158-165, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Whole-genome shotgun reads were analyzed to determine the repeat sequence composition in the genome of black mustard, Brassica nigra (L.) Koch. The analysis showed that satellite DNA sequences are very abundant in the black mustard genome. The distribution pattern of 7 new tandem repeats (BnSAT13, BnSAT28, BnSAT68, BnSAT76, BnSAT114, BnSAT180, and BnSAT200) on black mustard chromosomes was visualized using fluorescence in situ hybridization (FISH). The FISH signals of BnSAT13 and BnSAT76 provided useful cytogenetic markers; their position and fluorescence intensity allowed for unambiguous identification of all 8 somatic metaphase chromosomes. A karyotype showing the location and fluorescence intensity of these tandem repeat sequences together with the position of rDNAs and centromeric retrotransposons of Brassica (CRB) was constructed. The establishment of the FISH-based karyotype in B. nigra provides valuable information that can be used in detailed analyses of B. nigra accessions and derived allopolyploid Brassica species containing the B genome.
[Mh] Termos MeSH primário: DNA de Plantas/genética
Hibridização in Situ Fluorescente/métodos
Cariótipo
Mostardeira/genética
Sequências de Repetição em Tandem/genética
[Mh] Termos MeSH secundário: Centrômero
Cromossomos de Plantas/genética
Cromossomos de Plantas/ultraestrutura
DNA Ribossômico/genética
DNA Satélite/genética
Marcadores Genéticos
Genoma de Planta
Metáfase
Microscopia de Fluorescência
Retroelementos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal); 0 (DNA, Satellite); 0 (Genetic Markers); 0 (Retroelements)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE
[do] DOI:10.1159/000479179


  7 / 6863 MEDLINE  
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[PMID]:28738367
[Au] Autor:Colomina V; Catalan J; Britton-Davidian J; Veyrunes F
[Ad] Endereço:Institut des Sciences de l'Evolution (ISEM), UMR 5554, CNRS/Université Montpellier/IRD/EPHE, Montpellier, France.
[Ti] Título:Extensive Amplification of Telomeric Repeats in the Karyotypically Highly Diverse African Pygmy Mice.
[So] Source:Cytogenet Genome Res;152(2):55-64, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Telomeres are ribonucleoprotein structures protecting the physical ends of eukaryotic chromosomes. However, telomeric sequences can also occur at non-terminal regions of chromosomes, forming the so-called interstitial telomeric sequences (ITSs). Some ITSs are considered as relics of past chromosomal rearrangements and as such provide important insights into karyotype evolution. By FISH, we explored the distribution of telomeric motifs in the genome of a complex of mammalian species that has long been recognized for its extraordinary karyotypic diversity: the African pygmy mice. This survey involved 5 species, representing 10 highly diverse karyotypes with or without autosomal and sex-autosome robertsonian (Rb) fusions. The study revealed that in species with an ancestral-like karyotype (i.e., no fusions; Mus mattheyi and M. indutus), only terminal telomeres were observed, whereas in species experiencing intense chromosomal evolution (e.g., M. minutoides, M. musculoides), a large amplification of telomeric repeats was also identified in the pericentromeric region of acrocentrics and most metacentrics. We concluded that (i) the mechanism of Rb fusion in the African pygmy mice is different than the one highlighted in the house mouse; (ii) the intensity of the ITS hybridization signal could be a signature of the age of formation of the Rb fusion; (iii) the large amplification of pericentromeric telomeric sequences in acrocentrics may mediate the formation of Rb fusions, and (iv) the ITSs on the sex-autosome fusion Rb(X.1) may participate to the insulation buffer between the sexual and autosomal arms to prevent X inactivation from spreading and silencing autosomal genes and allow the independent regulation of replication timing of both segments.
[Mh] Termos MeSH primário: Amplificação de Genes
Cariótipo
Sequências Repetitivas de Ácido Nucleico/genética
Telômero/genética
[Mh] Termos MeSH secundário: Animais
Sequência de Bases
Feminino
Hibridização in Situ Fluorescente
Masculino
Metáfase/genética
Camundongos
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170725
[St] Status:MEDLINE
[do] DOI:10.1159/000478297


  8 / 6863 MEDLINE  
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[PMID]:28719910
[Au] Autor:Bustamante FO; Aliyeva-Schnorr L; Fuchs J; Beier S; Houben A
[Ad] Endereço:Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, Seeland, Germany.
[Ti] Título:Correlating the Genetic and Physical Map of Barley Chromosome 3H Revealed Limitations of the FISH-Based Mapping of Nearby Single-Copy Probes Caused by the Dynamic Structure of Metaphase Chromosomes.
[So] Source:Cytogenet Genome Res;152(2):90-96, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Genetic maps are based on the recombination frequency of molecular markers which often show different positions in comparison to the corresponding physical maps. To decipher the position and order of DNA sequences genetically mapped to terminal and interstitial regions of barley (Hordeum vulgare) chromosome 3H, fluorescence in situ hybridization (FISH) on mitotic metaphase chromosomes was performed with 16 genomic single-copy probes derived from fingerprinted BAC contigs. Long genetic distances at subterminal regions translated into short physical distances, confirming that recombination events occur more often at distal regions of chromosome 3H. Nonoverlapping FISH signals were frequently obtained for probes with a physical distance of at least 30-60 kb. Only 8% of the analyzed chromosomes showed a symmetric order of FISH signals on both sister chromatids. Due to the dynamic packing of metaphase chromatin, the order of 2 adjacent single-copy signals along the chromosome arms outside the (peri)centromeric region can only reliably be determined if the cytological distance is approximately 3%, corresponding to 21.6 Mb.
[Mh] Termos MeSH primário: Mapeamento Cromossômico/métodos
Cromossomos de Plantas/genética
Dosagem de Genes
Hordeum/genética
Hibridização in Situ Fluorescente/métodos
Metáfase/genética
Mapeamento Físico do Cromossomo/métodos
[Mh] Termos MeSH secundário: Pareamento de Bases/genética
Cromátides/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1159/000478631


  9 / 6863 MEDLINE  
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[PMID]:28714971
[Au] Autor:Lengefeld J; Hotz M; Rollins M; Baetz K; Barral Y
[Ad] Endereço:Institute of Biochemistry, ETH Zurich, Otto-Stern-Weg 3, 8093 Zurich, Switzerland.
[Ti] Título:Budding yeast Wee1 distinguishes spindle pole bodies to guide their pattern of age-dependent segregation.
[So] Source:Nat Cell Biol;19(8):941-951, 2017 Aug.
[Is] ISSN:1476-4679
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Many asymmetrically dividing cells unequally partition cellular structures according to age. Yet, it is unclear how cells differentiate pre-existing from newly synthesized material. Yeast cells segregate the spindle pole body (SPB, centrosome equivalent) inherited from the previous mitosis to the bud, while keeping the new one in the mother cell. Here, we show that the SPB inheritance network (SPIN), comprising the kinases Swe1 (also known as Wee1) and Kin3 (also known as Nek2) and the acetyltransferase NuA4 (also known as Tip60), distinguishes pre-existing from new SPBs. Swe1 phosphorylated Nud1 (orthologous to Centriolin) on young SPBs as they turned into pre-existing ones. The subsequent inactivation of Swe1 protected newly assembling SPBs from being marked. Kin3 and NuA4 maintained age marks on SPBs through following divisions. Downstream of SPIN, the Hippo regulator Bfa1-Bub2 bound the marked SPB, directed the spindle-positioning protein Kar9 towards it and drove its partition to the bud. Thus, coordination of SPIN activity and SPB assembly encodes age onto SPBs to enable their age-dependent segregation.
[Mh] Termos MeSH primário: Proteínas de Ciclo Celular/metabolismo
Segregação de Cromossomos
Cromossomos Fúngicos
Proteínas Tirosina Quinases/metabolismo
Proteínas de Saccharomyces cerevisiae/metabolismo
Saccharomyces cerevisiae/metabolismo
Fuso Acromático/metabolismo
[Mh] Termos MeSH secundário: Proteínas de Ciclo Celular/genética
Proliferação Celular
Proteínas do Citoesqueleto/genética
Proteínas do Citoesqueleto/metabolismo
Desoxirribonucleases/genética
Desoxirribonucleases/metabolismo
Fase G1
Regulação Fúngica da Expressão Gênica
Histona Acetiltransferases/genética
Histona Acetiltransferases/metabolismo
Metáfase
Proteínas Nucleares/genética
Proteínas Nucleares/metabolismo
Fosforilação
Proteínas Serina-Treonina Quinases/genética
Proteínas Serina-Treonina Quinases/metabolismo
Proteínas Tirosina Quinases/genética
Saccharomyces cerevisiae/genética
Saccharomyces cerevisiae/crescimento & desenvolvimento
Proteínas de Saccharomyces cerevisiae/genética
Transdução de Sinais
Fatores de Tempo
tRNA Metiltransferases/genética
tRNA Metiltransferases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BFA1 protein, S cerevisiae); 0 (BUB2 protein, S cerevisiae); 0 (Cell Cycle Proteins); 0 (Cytoskeletal Proteins); 0 (KAR9 protein, S cerevisiae); 0 (Nuclear Proteins); 0 (Saccharomyces cerevisiae Proteins); EC 2.1.1.- (tRNA Methyltransferases); EC 2.3.1.48 (Histone Acetyltransferases); EC 2.3.1.48 (NuA4 protein, S cerevisiae); EC 2.7.1.- (SWE1 protein, S cerevisiae); EC 2.7.10.1 (Protein-Tyrosine Kinases); EC 2.7.11.1 (KIN3 protein, S cerevisiae); EC 2.7.11.1 (Protein-Serine-Threonine Kinases); EC 3.1.- (Deoxyribonucleases); EC 3.1.- (TRM2 protein, S cerevisiae)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1038/ncb3576


  10 / 6863 MEDLINE  
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[PMID]:28709003
[Au] Autor:Ke Y; Xu Y; Chen X; Feng S; Liu Z; Sun Y; Yao X; Li F; Zhu W; Gao L; Chen H; Du Z; Xie W; Xu X; Huang X; Liu J
[Ad] Endereço:CAS Key Laboratory of Genome Sciences and Information, Collaborative Innovation Center of Genetics and Development, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China; University of Chinese Academy of Sciences, Beijing, China.
[Ti] Título:3D Chromatin Structures of Mature Gametes and Structural Reprogramming during Mammalian Embryogenesis.
[So] Source:Cell;170(2):367-381.e20, 2017 Jul 13.
[Is] ISSN:1097-4172
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High-order chromatin structure plays important roles in gene expression regulation. Knowledge of the dynamics of 3D chromatin structures during mammalian embryo development remains limited. We report the 3D chromatin architecture of mouse gametes and early embryos using an optimized Hi-C method with low-cell samples. We find that mature oocytes at the metaphase II stage do not have topologically associated domains (TADs). In sperm, extra-long-range interactions (>4 Mb) and interchromosomal interactions occur frequently. The high-order structures of both the paternal and maternal genomes in zygotes and two-cell embryos are obscure but are gradually re-established through development. The establishment of the TAD structure requires DNA replication but not zygotic genome activation. Furthermore, unmethylated CpGs are enriched in A compartment, and methylation levels are decreased to a greater extent in A compartment than in B compartment in embryos. In summary, the global reprogramming of chromatin architecture occurs during early mammalian development.
[Mh] Termos MeSH primário: Cromatina/metabolismo
Embrião de Mamíferos/metabolismo
Desenvolvimento Embrionário
[Mh] Termos MeSH secundário: Animais
Cromatina/química
Ilhas de CpG
Metilação de DNA
Replicação do DNA
Embrião de Mamíferos/química
Epigênese Genética
Feminino
Células Germinativas/metabolismo
Masculino
Metáfase
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Endogâmicos DBA
Oócitos/citologia
Espermatozoides/metabolismo
Zigoto/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Chromatin)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE



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