Base de dados : IBECS
Pesquisa : G16.500.275.157.812 [Categoria DeCs]
Referências encontradas : 4 [refinar]
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Fotocópia
Id: 164540
Autor: Bañeras, Lluís; Ruiz-Rueda, Olaya; López-Flores, Rocío; Quintana, Xavier D; Hallin, Sara.
Título: The role of plant type and salinity in the selection for the denitrifying community structure in the rhizosphere of wetland vegetation
Fonte: Int. microbiol;15(2):89-99, jun. 2012. ilus, tab, graf.
Idioma: en.
Resumo: Coastal wetlands, as transient links from terrestrial to marine environments, are important for nitrogen removal by denitrification. Denitrification strongly depends on both the presence of emergent plants and the denitrifier communities selected by different plant species. In this study, the effects of vegetation and habitat heterogeneity on the community of denitrifying bacteria were investigated in nine coastal wetlands in two preserved areas of Spain. Sampling locations were selected to cover a range of salinity (0.81 to 31.3 mS/cm) and nitrate concentrations (0.1 to 303 μM NO3-), allowing the evaluation of environmental variables that select for denitrifier communities in the rhizosphere of Phragmites sp., Ruppia sp., and Paspalum sp. Potential nitrate reduction rates were found to be dependent on the sampling time and plant species and related to the denitrifier community structure, which was assessed by terminal restriction fragment length polymorphism analysis of the functional genes nirS, nirK and nosZ. The results showed that denitrifier community structure was also governed by plant species and salinity, with significant influences of other variables, such as sampling time and location. Ruppia sp. and Phragmites sp. selected for certain communities, whereas this was not the case for Paspalum sp. The plant species effect was strongest on nirK-type denitrifiers, whereas water carbon content was a significant factor defining the structure of the nosZ-harboring community. The differences recognized using the three functional gene markers indicated that different drivers act on denitrifying populations capable of complete denitrification, compared to the overall denitrifier community. This finding may have implications for emissions of the greenhouse gas nitrous oxide (AU)

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Texto completo SciELO Brasil
Texto completo
Id: 164430
Autor: Corbella, Clara; Steidl, Rebecca P; Puigagut, Jaume; Reguera, Gemma.
Título: Electrochemical characterization of Geobacter lovleyi identifies limitations of microbial fuel cell performance in constructed wetlands
Fonte: Int. microbiol;20(2):55-64, jun. 2017. ilus, graf.
Idioma: en.
Resumo: Power generation in microbial fuel cells implemented in constructed wetlands (CW-MFCs) is low despite the enrichment of anode electricigens most closely related to Geobacter lovleyi. Using the model representative G. lovleyi strain SZ, we show that acetate, but not formate or lactate, can be oxidized efficiently but growth is limited by the high sensitivity of the bacterium to oxygen. Acetate and highly reducing conditions also supported the growth of anode biofilms but only at optimal anode potentials (450 mV vs. standard hydrogen electrode). Still, electrode coverage was poor and current densities, low, consistent with the lack of key c-type cytochromes. The results suggest that the low oxygen tolerance of G. lovleyi and inability to efficiently colonize and form electroactive biofilms on the electrodes while oxidizing the range of electron donors available in constructed wetlands limits MFC performance. The implications of these findings for the optimization of CW-MFCs are discussed (AU)

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Fotocópia
Id: 126425
Autor: López-Pérez, Marcos; Mirete, Salvador; Jardón-Valadez, Eduardo; González-Pastor, José E.
Título: Identification and modeling of a novel chloramphenicol resistance protein detected by functional metagenomics in a wetland of Lerma, Mexico
Fonte: Int. microbiol;16(2):103-111, jun. 2013. ilus, tab.
Idioma: en.
Resumo: The exploration of novel antibiotic resistance determinants in a particular environment may be limited because of the presence of uncultured microorganisms. In this work, a culture independent approach based on functional metagenomics was applied to search for chloramphenicol resistance genes in agro-industrial wastewater in Lerma de Villada, Mexico. To this end, a metagenomic library was generated in Escherichia coli DH10B containing DNA isolated from environmental samples of the residual arsenic-enriched (10 mg/ml) effluent. One resistant clone was detected in this library and further analyzed. An open reading frame similar to a multidrug resistance protein from Aeromonas salmonicida and responsible for chloramphenicol resistance was identifi ed, sequenced, and found to encode a member of the major facilitator superfamily (MFS). Our results also showed that the expression of this gene restored streptomycin sensitivity in E. coli DH10B cells. To gain further insight into the phenotype of this MFS family member, we developed a model of the membrane protein multiporter that, in addition, may serve as a template for developing new antibiotics (AU)

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Fotocópia
Id: 23766
Autor: Ladero, Miguel; Asensi, Alfredo; Díezgarretas, Blanca; Valle, Francisco.
Título: Estudios sobre la vegetación del entorno del Balneario de Alhama de Granada (Granada) / Study about the surroundings of Alhama de Granada Baths
Fonte: An Real Acad Farm = An. R. Acad. Farm. (Internet) = An. R. Acad. Farm;68(supl.1):425-445, abr. 2002. tab.
Idioma: Es.
Resumo: Se estudian los aspectos más significativos sobre la bioclimatología, biogeografía, geología y edafología del entorno del Balneario de Alhama de Granada (Granada, España), señalando la vegetación climática y sus principales etapas de sustitución. Se incluye un conjunto de referencias históricas (AU)
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