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Barcellos, David E. S. N
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Id: biblio-828209
Autor: Marks, Fernanda S; Almeida, Laura L; Driemeier, David; Canal, Cláudio; Barcellos, David E. S. N; Guimarães, Jorge A; Reck, José.
Título: Porcine circovirus 2 (PCV2) increases the expression of endothelial adhesion/junction molecules
Fuente: Braz. j. microbiol;47(4):870-875, Oct.-Dec. 2016. graf.
Idioma: en.
Resumen: Abstract Porcine circovirus type 2 (PCV2) is the primary causative agent of porcine circovirus disease, a complex multisystem syndrome in domestic pigs. Despite the significant economic losses caused by porcine circovirus disease, the mechanisms of pathogenesis underlying the clinical findings remain largely unclear. As various reports have highlighted the potential key role of vascular lesions in the pathogenesis of porcine circovirus disease, the aim of this work was to investigate effects of PCV2 infection on vascular endothelial cells, focusing on cell viability and expression of adhesion/junction molecules. PCV2 infection reduced endothelial cell viability, while viral infection did not affected the viability of several other classical cell lines. Also, PCV2 infection in endothelial cells displayed a dual/biphasic effect: initially, infection increased ICAM-1 expression, which can favor leukocyte recruitment and emigration to tissues and possibly inducing characteristic porcine circovirus disease inflammatory lesions; then, secondarily, infection caused an increase in zonula occludens 1 tight junction protein (ZO-1) expression, which in turn can result in difficulties for cell traffic across the endothelium and a potential impairment the immune response in peripheral tissues. These virus-induced endothelial changes could directly impact the inflammatory process of porcine circovirus disease and associated vascular/immune system disturbances. Data suggest that, among the wide range of effects induced by PCV2 on the host, endothelial modulation can be a pivotal process which can help to explain PCV2 pathogenesis in some porcine circovirus disease presentations.
Responsable: BR1.1 - BIREME


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Mores, Nelson
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Id: biblio-889245
Autor: Gava, Danielle; Serrão, Vitor Hugo Balasco; Fernandes, Lana Teixeira; Cantão, Mauricio Egídio; Ciacci-Zanella, Janice Reis; Morés, Nelson; Schaefer, Rejane.
Título: Structure analysis of capsid protein of Porcine circovirus type 2 from pigs with systemic disease
Fuente: Braz. j. microbiol;49(2):351-357, Apr.-June 2018. graf.
Idioma: en.
Proyecto: Brazilian Agricultural Research Corporation-EMBRAPA; . São Paulo Research Foundation-FAPESP.
Resumen: Abstract Economic losses with high mortality rate associated with Porcine circovirus type 2 (PCV2) is reported worldwide. PCV2 commercial vaccine was introduced in 2006 in U.S. and in 2008 in Brazil. Although PCV2 vaccines have been widely used, cases of PCV2 systemic disease have been reported in the last years. Eleven nursery or fattening pigs suffering from PCV2 systemic disease were selected from eight PCV2-vaccinated farms with historical records of PCV2 systemic disease in Southern Brazil. PCV2 genomes were amplified and sequenced from lymph node samples of selected pigs. The comparison among the ORF2 amino acid sequences of PCV2 isolates revealed three amino acid substitutions in the positions F57I, N178S and A190T, respectively. Using molecular modeling, a structural model for the capsid protein of PCV2 was built. Afterwards, the mutated residues positions were identified in the model. The structural analysis of the mutated residues showed that the external residue 190 is close to an important predicted region for antibodies recognition. Therefore, changes in the viral protein conformation might lead to an inefficient antibody binding and this could be a relevant mechanism underlying the recent vaccine failures observed in swine farms in Brazil.
Responsable: BR1.1 - BIREME


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Richtzenhain, Leonardo José
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Id: biblio-887831
Autor: Castro, Alessandra Marnie Martins Gomes de; Bersano, Josete Garcia; Souza, Sheila Oliveira Silva; Ogata, Renato Akio; Nara, Julia Mitico; Richtzenhain, Leonardo José.
Título: Circovírus suíno 2b: isolamento e taxa de mutação em linhagem de célula de macrófagos (J744) / Porcine circovirus 2b: isolations and mutation rate in J744 lineage macrophage cell
Fuente: Arq. Inst. Biol;84:e1012014, 2017. graf, ilus.
Idioma: pt.
Proyecto: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP); . Bolsa Jovem Pesquisador.
Resumen: Porcine circovirus 2 (PCV2) está associado a vários sinais clínicos que são designados coletivamente como Circovirose e tem grande impacto na suinocultura. O isolamento viral é classicamente realizado em células da linhagem PK-15, contudo outras células têm sido testadas. Apesar dos avanços nos estudos com PCV2, o isolamento ainda é um desafio. Diante da dificuldade de manutenção dessas linhagens celulares comumente utilizadas associadas à necessidade do uso de substâncias tóxicas para o isolamento de PCV2, os objetivos do presente trabalho foram descrever o primeiro isolamento de Porcine circovirus 2b em linhagens de células de macrófago (J744) e verificar a taxa de mutação nesse sistema. Uma amostra de pulmão foi submetida ao sequenciamento e agrupada ao genótipo PCV2b. Essa amostra foi utilizada para inocular uma garrafa de J744 (com 30% de confluência em meio RPMI com 10% de soro fetal bovino) e submetida a cinco passagens, as quais foram acompanhadas por reação em cadeia da polimerase quantitativa (PCRq). As cargas virais inicial e final foram de 2,90 × 103 e de 4,45 × 108 cópias de DNA/µL para PCV2b, respectivamente. O sequenciamento confirmou o isolamento e descartou o coisolamento de mais de um genótipo. Após cinco passagens, o isolado apresentou identidade de 99,7%, com descrição de cinco mutações pontuais, uma sinônima e quatro não sinônimas, observadas nas regiões do gene cap e rep. Os resultados obtidos demonstram que as células J744 apresentam a susceptibilidade, e a instabilidade do vírus em J744 será importante para a compreensão do vírus.(AU)

Porcine circovirus 2 (PCV2) is associated with various clinical signs that are collectively designated as Circovirosis and has a great impact on the pig industry. The virus isolation is classically performed on PK-15 cell line, but other cells have been tested. Despite advances in studies with PCV2, isolation is still a challenge. The difficulty of maintaining these cell lines commonly used associated with the use of toxic substances to the isolation of PCV2 had stimulated the present study, that had the objectives to describe the first isolation of PCV2b in macrophage cell lines, J744 and verify the mutation rate at this system. A sample of lung was pooled and submitted to sequencing in which was classified in genotype PCV2b. This sample was used to inoculate a bottle of J744 with 30% of confluence in RPMI with 10% fetal bovine serum and submitted to five passages, which were accompanied by chain reaction quantitative polymerase (PCRq). The initial and final viral loads were 2.90 × 103 and 4.45 × 108 DNA copies/µL for PCV2b, respectively. Sequencing confirmed the isolation and had eliminated possible co-isolation of more than one genotype. After five passages, the isolate showed 99.7% identity with description of five point of non-synonymous or/and synonymous mutations observed in the cap and rep gene. The results demonstrate that J744 cells exhibit susceptibility, and the instability of the virus in J744 will be important for understanding the virus.(AU)
Responsable: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Brito, Wilia Marta Elsner Diederichsen de
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Id: biblio-887846
Autor: Alvarenga, Thales Coelho de; Brito, Wilia Marta Elsner Diederichsen de; Ruiz, Vera Letticie de Azevedo; Castro, Alessandra Marnie Martins Gomes de; Bersano, Josete Garcia; Ogata, Renato Akio; Soares, Patrícia.
Título: Micoplasma hyopneumoniae associado ao circovírus suíno tipo 2 em plantéis não vacinados para micoplasmose / Mycoplasma hyopneumoniae associated with porcine circovirus type 2 in non-vaccinated herds for mycoplasmosis
Fuente: Arq. Inst. Biol;84:e0442016, 2017. ilus.
Idioma: pt.
Resumen: A síndrome circovirose suína e doenças associadas (PCVAD) tem sido descrita em diversas regiões do mundo. Seu agente primário, o circovírus suíno tipo 2 (PCV2), está associado a elevados índices de refugagem nas granjas e a vultuosos prejuízos econômicos. Diversos fatores de risco estão relacionados à manifestação dos quadros clínicos da síndrome, nomeadamente deficiências de manejo, presença de coinfecções e imunização diante do agente. Entre os agentes frequentemente relatados associados ao PCV2 está o Mycoplasma hyopneumoniae. Este estudo objetivou verificar a ocorrência de M. hyopneumoniae em animais diagnosticados estarem acometidos pela PCVAD, em sistemas intensivos de produção de suínos do estado de Goiás. Amostras de secreção nasal de 40 animais foram analisadas para a pesquisa do DNA de M. hyopneumoniae. Do total das amostras de secreção nasal, 6 (15%) foram positivas na reação em cadeia da polimerase (PCR) para o M. hyopneumoniae, apenas em granjas que não praticavam a vacinação contra esse agente. Os resultados relacionados à presença de micoplasma estão de acordo com os achados clínicos dos animais analisados que apresentavam sintomatologia de doenças respiratórias e lesões relacionadas ao trato respiratório. Este é o primeiro relato da associação de PCV2 com M. hyopneumoniae em suínos identificados com PCVAD no estado de Goiás.(AU)

Porcine circovirus associated diseases (PCVAD) have been reported around the world. They are associated with high culling rates and large economic losses. Porcine circovirus type 2 (PCV2) is the primary causative agent. Several risk factors are related to the manifestation of clinical syndrome, including deficiencies of management, presence of co-infections and immunization against involved agents. Mycoplasma hyopneumoniae is often reported as an agent associated to PCV2 infections. The aim of this study was to verify the occurrence of M. hyopneumoniae in animals diagnosed with PCVAD in intensive pig farming systems in Goiás, Brazil. Forty nasal secretion samples were collected for M. hyopneumoniae DNA detection by polymerase chain reaction (PCR). Out of this, 6 (15%) were positive for M. hyopneumoniae DNA. All positive samples were collected from animals in non-vaccinated herds. Mycoplasma has been detected in animals showing clinical signs and lesions of respiratory diseases. To our knowledge, this is the first report of PCV2 association with M. hyopneumoniae in pigs with PCVAD identified in the state of Goiás, Brazil.(AU)
Responsable: BR1942.1 - NID - Biblioteca - Núcleo de Informação e Documentação


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Id: lil-656668
Autor: Castro, A. M. M.G; Castro Junior, F. G; Budiño, F. E. L; Baldin, C. M; Silva, S. O. S; Brandão, P. E; Richtzenhain, L. J.
Título: Detection of genetic characterization of Porcine circovirus 2 (PCV2) in Brazilian wildlife boars
Fuente: Braz. j. microbiol;43(3):1022-1025, July-Sept. 2012. ilus.
Idioma: en.
Resumen: A semi-intensive wildlife boars farm presented a clinical history of high mortality in 70 - 90 days-old pigs (> 50 %). Two 90 days-old animals with weight loss and wasting were necropsied and the samples tested for PCV2 by polymerase chain reaction (PCR). The genetic material of PCV2 was sequenced and classified into the PCV2a genotype together with PCV2 sequences obtained from samples of Poland, Brazil, Slovenia and Greece wild boars.
Responsable: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Id: lil-634468
Autor: Sarradell, J.; Pérez, A.M.; Comba, E.; Pereira, N.; Anthony, L.; Andrada, M.; Segalés, J..
Título: Hallazgos patológicos en cerdos afectados con el síndrome del desmedro multisistémico postdestete de la República Argentina / Pathological findings in pigs affected by the postweaning multisystemic wasting syndrome in Argentina
Fuente: Rev. argent. microbiol;36(3):118-124, jul.-sep. 2004. ilus, tab.
Idioma: es.
Resumen: El síndrome del desmedro multisistémico postdestete (PMWS) fue descrito por primera vez en Canadá en el año 1991 y desde entonces un número creciente de casos han sido diagnosticados en todo el mundo. En la Argentina, el PMWS fue reportado por primera vez recientemente. Se estudiaron 48 cerdos de 5 a 12 semanas de edad con signos característicos de PMWS procedentes de 19 granjas. Si bien se desconoce la distribución real del virus en nuestro país se observó desde el año 2001 un número creciente de granjas con PMWS y distribuidas en las principales provincias productoras. La histopatología fue una herramienta diagnóstica importante en casos sospechosos de PMWS con la observación de diferentes grados de lesión. En los animales estudiados las infecciones secundarias pudieron ser importantes, ya sea por patógenos oportunistas o por complicaciones bacterianas.

Postweaning multisystemic wasting syndrome was first described in Canada in 1991 and at present an increasing number of cases has been diagnosed worldwide. In Argentina the first cases of PMWS were reported recently. Forty eight 5 to 12 week old pigs with signs characteristic of PMWS from 19 farms were studied. Although the real distribution of the virus in our country is not known it was observed an increasing number of farms with PMWS distributed in the major producing provinces. The histopathology was an important tool in diagnosis of suspicious cases of PMWS with the observation of different degrees of lesion. In the studied animals, the secondary infections, either by opportunistic pathogens or secondary bacteria could be important.
Responsable: AR1.2 - Instituto de Investigaciónes Epidemiológicas


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Id: lil-607549
Autor: Silva, Fernanda Miquelitto Figueira da; Silva Júnior, Abelardo; Peternelli, Ethel Fernandes de Oliveira; Viana, Vinícius Winter; Chiarelli Neto, Orlando; Fietto, Juliana Lopes Rangel; Vargas, Marlene Izabel; Nero, Luís Augusto; Almeida, Márcia Rogéria de.
Título: Retrospective study on Porcine circovirus-2 by nested pcr and real time pcr in archived tissues from 1978 in Brazil
Fuente: Braz. j. microbiol;42(3):1156-1160, July-Sept. 2011. ilus, tab.
Idioma: en.
Resumen: Porcine circovirus-2 (PCV-2) infection is currently considered an important disease of swine. The pathogenic agent was first described in Brazil in 2000. This study detected the PCV-2 DNA in four Brazilian pig tissues collected between 1978 and 1979. This observation is the oldest description of this virus in Brazil.
Responsable: BR32.1 - Serviço de Biblioteca e Informação Biomédica


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Mores, Nelson
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Id: lil-479859
Autor: Gava, Danielle; Zanella, Eraldo L; Morés, Nelson; Ciacci-Zanella, Janice R.
Título: Transmission of porcine circovirus 2 (PCV2) by semen and viral distribution in different piglet tissues
Fuente: Pesqui. vet. bras = Braz. j. vet. res;28(1):70-76, jan. 2008. ilus, tab.
Idioma: en.
Proyecto: Embrapa; . CNPq; . CAPES.
Resumen: Porcine circovirus infections are caused by the porcine circovirus 2 (PCV2). Among six different clinical manifestations involving respiratory, enteric, nervous and reproductive signs, the postweaning multisystemic wasting syndrome (PMWS) is the most important and studied disease. However, reproductive failures associated with PCV2 have been increasingly reported. Some studies have shown the possible contamination of sows by semen of PCV2 positive boars. In order to investigate the transmission of PCV2 by contaminated semen and its ability to infect the sow and piglets, 20 PCV2 negative sows were inseminated, 10 with negative boar semen and 10 with previously nested-PCR tested positive boar semen. The sows were weekly monitored and blood samples were collected. Based on the results, 4 out 20 sows were selected (1 sow was PCR negative and inseminated with a negative semen, 2 sows were PCR negative and inseminated with a positive semen and 1 sow was PCR negative and inseminated with a positive semen, but became PCR positive around the 30 days of pregnancy). After weaning, 12 male piglets, 3 of each sow, were selected and maintained under isolation. In order to investigate which organs harbored the virus, the young pigs were necropsied around 9 months of age. Samples of serum collected monthly were tested by immunocitochemistry (ICC), and all 12 pigs serum converted. Samples of lymphoid, systemic and reproductive organs were analyzed by nested-PCR and immunohistochemistry (IHC). Evaluation of the samples by nested-PCR, revealed that several tissues were positive in 10 of 12 pigs, mainly the lymph nodes, bone marrow and spleen. Various samples were positive by IHC in 8 of 12 piglets, being the lymph nodes, tonsils and bulbourethral glands the most frequently positive. Thus, the results of testing different samples, in the 3 tests (ICC, nested-PCR and IHC) were complementary. These results show that PCV2 transmission through semen to the sows...
Responsable: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-414419
Autor: França, Ticiana do Nascimento; Ribeiro, Carlos Torres; Cunha, Bernardo Melo da; Peixoto, Paulo Vargas.
Título: Circovirose suína / Porcine circovirosis: a review
Fuente: Pesqui. vet. bras = Braz. j. vet. res;25(2):59-72, abr.-jun. 2005.
Idioma: pt.
Resumen: Por meio de revisão da literatura pertinente foram coligidos e são apresentados os principais dados relativos aos aspectos epidemiológicos, clínicos, anátomo e histopatológicos observados na infecção por Circovírus Porcino tipo 2 (PCV-2) em suínos. São abordados a Síndrome Definhante Multissistêmica dos Suínos Desmamados (SDMDS), o Tremor Congênito Suíno (TCS), a Síndrome da Nefropatia e Dermatite Porcina (SNDP), bem como outras enfermidades associadas ou correlatas, a Síndrome Respiratória e Reprodutiva Porcina (SRRP), a Pneumonia Necrotizante Proliferativa (PNP) e as falhas reprodutivas. Uma vez que a SDMSD já foi registrada na Região Sul do Brasil e no Estado do Rio de Janeiro esse estudo objetiva chamar a atenção para o especial significado dessa virose para a suinocultura brasileira, em função dos prejuízos econômicos por ela determinados.
Responsable: BR68.1 - Biblioteca Virginie Buff D'Ápice


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Id: lil-363804
Autor: Canal, Cláudio W; Ferreira, Danilo José; Macagnan, Marisa; Fallavena, Luiz C. B; Moraes, Hamilton L. S; Wald, Vera B.
Título: Prevalence of antibodies against chicken anaemia virus (CAV) in broiler breeders in Southern Brazil
Fuente: Pesqui. vet. bras = Braz. j. vet. res;24(2):89-92, Apr.-June 2004. tab, graf.
Idioma: en.
Resumen: A doença clínica causada pelo vírus da anemia das galinhas (CAV) ocorre quando os pintos são infectados durante as primeiras duas semanas de vida e pode ser prevenida se as matrizes transferirem anticorpos suficientes para a sua progênie. Em vista disso, este estudo foi realizado visando determinar a prevalência de anticorpos contra o CAV em alguns lotes de matrizes pesadas no Brasil. Buscou-se ainda verificar em que fase da vida as reprodutoras seriam infectadas e quais seriam os títulos de anticorpos nessas aves. Um total de 1709 amostras de soro de 64 lotes de reprodutoras não vacinadas e 12 lotes de reprodutoras vacinadas contra o CAV foram analisados por ELISA. Todos os lotes de aves não vacinadas apresentaram anticorpos. Dentre esses, 89 por cento dos indivíduos foram positivos, 52 por cento com títulos de anticorpos capazes de conferir proteção a sua progênie contra o CAV. Igualmente, todos os lotes de matrizes vacinadas apresentaram anticorpos contra o CAV em títulos considerados protetores para a progênie. Conclui-se que a vacinação das reprodutoras parece capaz de conferir proteção aos pintinhos contra doença associada ao CAV.
Responsable: BR68.1 - Biblioteca Virginie Buff D'Ápice



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