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Id: biblio-1154102
Autor: Gomes, Vilessa Lilian de Araújo; Braz, Janine Karla França da Silva; Martins, Gabriel Moura; Clebis, Naianne Kelly; Oliveira, Moacir Franco de; Morais, Danielle Barbosa; Moura, Carlos Eduardo Bezerra de.
Título: Identification of telocytes in dystrophic mice testis / Identificação de telócitos em testículos de camundongos distróficos
Fonte: Einstein (Säo Paulo);19:eAI5737, 2021. graf.
Idioma: en.
Descritores: Testículo/ultraestrutura
Telócitos/ultraestrutura
Células Intersticiais do Testículo
-Testículo/citologia
Camundongos Endogâmicos mdx
Microscopia Eletrônica de Transmissão/veterinária
Telopódios/fisiologia
Telopódios/ultraestrutura
Telócitos/fisiologia
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: biblio-838959
Autor: Zhang, Tianxiang; Peng, Dong; Qi, Lei; Li, Weixuan; Fan, Mengyuan; Shen, Jiachen; Yang, Liangliang; Wang, Yihua; Wang, Wenxia; Hu, Xiaolong; Cai, Ruibo; Zhou, Ran; Wei, Yuting; Zhou, Juntong; Yang, Shuang; Hu, Defu; Liu, Shuqiang.
Título: Musk gland seasonal development and musk secretion are regulated by the testis in muskrat (Ondatra zibethicus)
Fonte: Biol. Res;50:10, 2017. graf.
Idioma: en.
Projeto: China Postdoctoral Science Foundation; . China Postdoctoral Science Foundation; . Zhangzhou Pien Tze Huang Pharmaceutical Co., Ltd.
Resumo: BACKGROUND: The muskrat is a seasonal breeder. Males secrete musk to attract females during the breeding season. The testosterone binding to the androgen receptor (AR) in musk glands of muskrat may play an important role conducting the musk secretion process. METHODS: The musk gland, testis and blood samples of musk rats are collected in both breeding and non-breeding seasons. Some part of the samples are kept in liquid nitrogen for transcriptome analysis and Western blotting test. Some part of the samples are kept in 70% alcohol for histology experiment, blood samples are kept at -20 °C for the serum testosterone measurement experiment. RESULTS: This study demonstrates that the quantity of secreted musk, the volume of the musk glands, the diameter of the gland cells and AR expression are all higher during the breeding season than at other times (p < 0.01). StAR, P450scc and 3ß-HSD expression in the Leydig cells of the testis were also higher during this season, as was serum testosterone. AR was also observed in the gland cells of two other musk-secreting animals, the musk deer and small Indian civet, in their musk glands. These results suggest that the testes and musk glands co-develop seasonally. CONCLUSION: The musk glands' seasonal development and musk secretion are regulated by the testes, and testosterone plays an important role in the seasonal development of musk glands.
Descritores: Glândulas Odoríferas/crescimento & desenvolvimento
Glândulas Odoríferas/metabolismo
Testículo/metabolismo
Ácidos Graxos Monoinsaturados/metabolismo
-Tamanho do Órgão
Valores de Referência
Reprodução/fisiologia
Glândulas Odoríferas/anatomia & histologia
Estações do Ano
Testículo/crescimento & desenvolvimento
Testosterona/sangue
Cruzamento
Ensaio de Imunoadsorção Enzimática
Ácidos Graxos Monoinsaturados/análise
Imuno-Histoquímica
Receptores Androgênicos/análise
Receptores Androgênicos/metabolismo
Western Blotting
Arvicolinae
Análise de Sequência de RNA
Células Intersticiais do Testículo/metabolismo
Limites: Animais
Masculino
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-968970
Autor: Mehanna, Mahmoud; Ferreira, André Luís Souza; Ferreira, Adelina; Paz, Regina Célia Rodrigues da; Morgado, Thaís Oliveira.
Título: Histology of the testis and the epididymal ducts from hoary fox Lycalopex vetulus (Lund, 1842) / Histologia dos testículos e ductos epididimários da raposa-do-campo Lycalopex vetulus (Lund, 1842)
Fonte: Biosci. j. (Online);34(6):1697-1705, nov.-dec. 2018. ilus.
Idioma: en.
Resumo: The hoary fox Lycalopex vetulus (Lund, 1842) is a small canid, endemic to Brazil, belonging to the Canidae family, widely distributed in the country, occurring records in different regions and habitats. The objective of this study is to describe morphologically the testicles and epididymal ducts of hoary fox. The animals, coming from the zoo of Federal University of Mato Grosso, Brazil, had died by natural causes. The male reproductive system was dissected to collect the testicles. The samples were fragmented and histologically examined. Microscopically, the testes were coated by the vaginal and albuginea tunic, formed by modeled dense connective tissue with large amount of collagen fibers. Into the organ, convoluted seminiferous tubules were surrounded by a basement membrane characterized by the presence of myoid and Sertoli cells and germinative epithelium composed by Between the seminiferous tubules, interstitial tissue composed of connective tissue, blood and lymph vessels and Leydig cells in polyhedral shape was present. The epididymal ducts showed pseudostratified columnar epithelium with secretory cells, in which stereocilia located on a basement membrane filled by myoid cells were found. The structures observed by us are very similar to those described for other mammals.(AU)

A raposa-do-campo Lycalopex vetulus (Lund, 1842) é um canídeo de pequeno porte, endêmico do Brasil, pertencente a família Canidae, com ampla distribuição no país, ocorrendo registros em várias regiões e habitats diferentes. Com base nessa informação, o objetivo deste trabalho é caracterizar morfologicamente os testículos e ductos epididimários da raposa-do-campo. O animal, oriundo do zoológico da Universidade Federal de Mato Grosso, Brasil, veio a óbito por causas naturais e o sistema reprodutor masculino foi dissecado para coleta dos testículos. As amostras retiradas foram fragmentadas e histológicamente examinadas. A partir das análises microscópicas dos testículos foram identificados: a túnica vaginal e albugínea, formada por tecido conjuntivo denso modelado, com grande quantidade de fibras colágenas; túbulos seminíferos enovelados e revestidos por epitélio germinativo e células de Sertoli, envolvidos por uma membrana basal com presença de células mioides; tecido intersticial entre os túbulos seminíferos constituído de tecido conjuntivo, vasos sanguíneos e linfáticos, e células de Leydig em formato poliédrico. Os ductos epididimários apresentaram epitélio cilíndrico pseudoestratificado com células secretoras dos quais projetam estereocílios, situados sobre uma membrana basal repleta por células mióides. As estruturas por nós observadas possuem muita semelhança com as demais descrições para mamíferos.(AU)
Descritores: Testículo
Raposas
Genitália Masculina
-Túbulos Seminíferos
Células de Sertoli
Membrana Basal
Tecido Conjuntivo
Canidae
Estereocílios
Células Intersticiais do Testículo
Responsável: BR396.1 - Biblioteca Central


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Miglino, Maria Angélica
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Id: biblio-886766
Autor: SIMÕES, LUCIANA S; SASAHARA, TAIS H C; FAVARON, PHELIPE O; MARQUES JÚNIOR, RICARDO; OLIVEIRA, MOACIR F DE; MACHADO, MÁRCIA R F; MIGLINO, MARIA ANGELICA.
Título: The quantification of testicular cells during the postnatal development in two Caviomorph rodents: the guinea pig (Cavia porcellus) and the cutia (Dasyprocta agouti)
Fonte: An. acad. bras. ciênc;89(3):1745-1751, July-Sept. 2017. tab, graf.
Idioma: en.
Resumo: ABSTRACT The germinative, Sertoli and Leydig cells of two caviomorph rodents (Cavia porcellus and Dasyprocta agouti) were counted as well as the estimation of the total volume of the testis and the total volume of seminiferous tubules and interstitium in prepubertal, pubertal and adult animals. The number of spermatogonia, spermatocytes and spermatids cells increased during the pubertal phase in both rodents, notably the spermatid cells. The spermatocyte and spermatid slightly decreased in the adult of both rodents, but the increment in spermatogonia cells number was seen, mainly in cutias. The number of Sertoli cells increased in pubertal rodents, but in the adult the number reduced. Substantial number of Leydig cells was counted in pubertal and adult guinea pigs. In cutias, the number of Leydig cells increased in pubertal phase and decline in adults. The design-based stereological method has proven to be unbiased and reliable to be applied in reproduction studies.
Descritores: Células de Sertoli/citologia
Espermatozoides/citologia
Dasyproctidae/crescimento & desenvolvimento
Células Intersticiais do Testículo/citologia
-Espermatozoides/crescimento & desenvolvimento
Contagem de Células
Cobaias
Limites: Animais
Masculino
Responsável: BR1.1 - BIREME


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Id: lil-553338
Autor: Parmigiani, Raphael Bessa.
Título: Caracterização de um novo antígeno tumoral: CTSP-1 / Characterization of a new tumor antigen: CTSP-1.
Fonte: São Paulo; s.n; 2005. 140 p. ilus, tab.
Idioma: pt.
Tese: Apresentada a Fundação Antônio Prudente para obtenção do grau de Doutor.
Resumo: A necessidade de identificar novos antígenos tumorais que possam ser utilizados no tratamento e diagnóstico do câncer, tem levado ao desenvolvimento de técnicas cada vez mais eficientes na detecção dos mesmos. Antígenos de diferentes categorias foram identificados e caracterizados, sendo os antígenos cancer-testis (CT) e os antígenos de diferenciação (CD) os de maior importância clínica, dado seu restrito padrão de expressão. Utilizando uma estratégia de alinhamento de seqüências expressas no genoma humano, identificamos um novo transcrito localizado no cromossomo 21, denominado CTSP-1, que apresenta alta similaridade com o antígeno tumoral NY-BR-1... Além disso, avaliamos seu padrão de expressão em diferentes tecidos normais, linhagens celulares tumorais e amostras de tumores de pacientes... Através de immunoblotting foi possível a identificação de uma banda específica de 22kDa, correspondente ao peso esperado da proteína CTSP-1 em extrato total de testículo normal. Em seguida, através da imunohistoquímica, verificamos uma marcação preferencial nas espermatogônias e células de Leydig de testículo normal. Nos tecidos com amostras pareadas normal/tumor (mama e próstata), apenas as amostras tumorais foram fortemente marcadas. Posteriormente, a proteína CTSP-1 recombinante foi utilizada na investigação de anticorpos específicos em plasma de pacientes com câncer. Aproximadamente 150 amostras foram analisadas, das quais 20% apresentaram resposta imune humoral contra a proteína CTSP-1. Estes resultados revelam que o CTSP-1 é um novo antígeno tumoral da categoria dos CTs, com expressão restrita a tumor e testículo e com alta imunogenicidade em pacientes com câncer...(AU)

The need to identify new tumor antigens to be used in cancer treatment and diagnosis has lead to the development of efficient techniques for this purpose. Antigens from different categories have been identified and characterized and, among those, the cancer-testis (CT) and the cancer differentiation (CD) antigens are of the greatest clinicai interest dueto their restricted expression partem. Using alignments between expressed sequences and the Human Genome Sequence, we identified a new gene located on chromosome 21, named CTSP-1. This gene has a high similarity to the tumor antigen NY-BR-1, which encodes for a tissue specific transcription factor and is a potential target for cancer immunotherapy. In order to verify ifthe CTSP-1 gene is really a new tumor antigen, we performed its complete characterization. Using different techniques, we were able to obtain the complete sequence of the CTSP-1 gene and to identify different alternative polyadenilation and splicing forms. Moreover, we analyzed the CTSP-1 expression pattern in normal tissues, tumor cell lines and tumor samples. CTSP-1 showed a restricted expression pattern, being expressed only in testis among normal tissues, in different tumor celllines (9/22) and in different tumor types (7 4/178), which matches with the expression pattern of Cancer-Testis antigens. Afterwards, the recombinant CTSP-1 protein was expressed in a heterologous system and used for the generation of polyclonal antibody in mice. This antiboby was used in immunoblotting and immunohistochemistry experiments for the detection of CTSP-1 protein in normal testis and paired normal and tumor samples from breast and prostate. Using immunoblotting, a 22kDa specific band was identified in testis total protein extract, corresponding to the expected molecular weight of the CTSP-1 protein. Using immunohistochemistry, we verified the preferential staining of germ cells and Leydig cells in normal testis. Among tissues with paired normal/tumor samples, only tumor samples were strongly stained. The CTSP-1 recombinant protein was also used in the search for specific antibodies in plasma from cancer patients. Approximately 150 samples were analyzed, of which 20% showed a humoral immune response against the CTSP-1 protein. Taken together, these results confirm that the CTSP-1 gene is a new tumor antigen from the Cancer-Testis category, with restricted expression in testis and tumors and with high immunogenicity in cancer patients (AU)
Descritores: Antígenos
Bancos de Espécimes Biológicos
Immunoblotting
Imunoterapia
Poliadenilação
Processamento Alternativo
-Imuno-Histoquímica
Células Intersticiais do Testículo
Espermatogônias
Limites: Humanos
Masculino
Responsável: BR30.1 - Biblioteca
BR30.1


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Id: biblio-886170
Autor: Neves, Bruno Vinicius Duarte; Lorenzini, Fernando; Veronez, Djanira; Miranda, Eduardo Pereira de; Neves, Gabriela Duarte; Fraga, Rogério de.
Título: Numeric and volumetric changes in Leydig cells during aging of rats
Fonte: Acta cir. bras;32(10):807-815, Oct. 2017. tab, graf.
Idioma: en.
Resumo: Abstract Purpose: To analyze the effects of aging in rats on the nuclear volume, cytoplasmic volume, and total volume of Leydig cells, as well as their number. Methods: Seventy-two Wistar rats were divided into six subgroups of 12 rats, which underwent right orchiectomy at 3, 6, 9, 12, 18, and 24 months of age. The weight and volume of the resected testicles were assessed. A stereological study of Leydig cells was conducted, which included measurements of cell number and nuclear, cytoplasmic, and total cell volumes. Results: The weight and volume of the resected testicles showed reductions with age. Only the subgroup composed of 24-month old rats showed a decrease in the nuclear volume of Leydig cells. Significant reductions in the cytoplasmic volume and total volume of Leydig cells were observed in 18- and 24-month old rats. The number of Leydig cells did not vary significantly with age. Conclusions: Aging in rats resulted in reduction of the nuclear, cytoplasmic, and total cell volumes of Leydig cells. There was no change in the total number of these cells during aging.
Descritores: Envelhecimento/fisiologia
Células Intersticiais do Testículo/patologia
-Orquiectomia
Contagem de Células
Ratos Wistar
Limites: Animais
Masculino
Ratos
Responsável: BR1.1 - BIREME


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Id: biblio-954204
Autor: Ruiz, Paul; Genovese, Patricia; Bielli, Alejandro; Herrera, Emiliano; Pautassi, Ricardo Marcos.
Título: La reserpina aumenta la expresión de BDNF y PCNA, y disminuye la de caspasa-3, en células intersticiales (células de Leydig) de ratas / Reserpine increases BDNF and PCNA expression, but decreases caspase-3, in rat interstitial cells (Leydig cells)
Fonte: Int. j. morphol;36(3):895-900, Sept. 2018. tab, graf.
Idioma: es.
Projeto: Agencia Nacional de Investigación e Innovación; . Agencia Nacional de Promoción Científica y Tecnológica de Argentina.
Resumo: La reserpina es un antipsicótico e hipotensor arterial que reduce significativamente los niveles de monoaminas centrales, y también es utilizada para modelar los cuadros depresivos humanos en animales de laboratorio. Este trabajo estudió, en ratas Wistar machos adolescentes, cómo la reserpina afecta indicadores moleculares de la función testicular, la cual se ha visto alterada en humanos deprimidos. Una semana luego de finalizado el tratamiento con reserpina (4 dosis de 0,0 o 1,0 mg/Kg, cada 2 días) la respuesta ansiosa y depresiva fue evaluada en un laberinto en cruz elevado. Posteriormente, se sacrificaron los animales y disecaron los testículos, los cuales fueron fijados e incluidos en bloques de parafina de donde se obtuvieron cortes histológicos de 6 µm de espesor. Estos se utilizaron para medir el diámetro de los túbulos seminíferos y para medir por inmunohistoquímica el porcentaje de células intersticiales (células de Leydig) positivas a (1) Factor neurotrófico derivado del cerebro, (2) antígeno nuclear de células en proliferación (BDNF y PCNA, respectivamente, por sus siglas en inglés), y a (3) caspasa-3. Se obtuvo también un índice de positividad al receptor de andrógenos en las células intersticiales. La expresión del receptor de andrógeno fue evaluada utilizando una escala semicuantitativa de escores (0, 1, 2 y 3) y el resto de las moléculas por presencia o ausencia de expresión de cada antígeno investigado en 300 células por preparado. Los resultados comportamentales indicaron alteraciones en la respuesta de ansiedad y una significativa depresión motora (e.g., mayor latencia en conductas de escape del sector blanco) en los animales tratados con reserpina. No se observaron diferencias en los diámetros de los túbulos seminíferos ni en la expresión del receptor de andrógeno, mientras que sí se encontró mayor proporción de células intersticiales positivas a BDNF y PCNA, y menor proporción de células positivas a caspasa-3, en los animales tratados. Los resultados corroboran la capacidad de la reserpina para reproducir rasgos comportamentales de la depresión. La administración de la droga, sin embargo, no parece reproducir a nivel testicular los efectos deletéreos encontrados en humanos deprimidos, e incluso los resultados sugieren que la reserpina puede mejorar algunos aspectos de la funcionalidad testicular relacionadas con la actividad de las células intersticiales en ratas.

Reserpine, a drug that depletes central monoamines, has been used as an antipsychotic and arterial hypotensive, and to model depression in animals. The present study analyzed, in adolescent male rats, the effects of chronic reserpine treatment on molecular indexes of testicular function. A week after termination of the treatment (4 doses of 0,0 or 1,0 mg/Kg/every 48 h) the animals were tested for anxiety response and depression patterns in an elevated plus maze. They were then euthanized, their testes dissected, fixed and embedded in paraffin to obtain blocks. Histological sections (6 µm) were obtained and used to measure the diameter of seminiferous tubules and the expression in Leydig cells of Brain-derived neurotrophic factor (BDNF), Proliferating cell nuclear antigen (PCNA), Caspase-3 and androgen receptors, by immunohistochemistry. Behavioral results indicated significant alterations in anxiety responses and a significant motor depression (e.g., greater latency to escape from the white sector). There were no differences between groups in the diameter of seminiferous tubules nor in the androgen receptors positivity. Reserpine-treated animals, however, exhibited more BDNF and PCNA positive cells, and less positive Caspase-3 cells in Leydig cells, than control animals. The results corroborate the efficacy of reserpine to reproduce some of the behavioral components of depression. The drug, however, does not seem to exert in rats the same effects on testicular function that have been found in humans diagnosed with depression. Furthermore the drug seems to enhance some aspects of testicular function related to Leydig cells function in rats.
Descritores: Reserpina/farmacologia
Testículo/efeitos dos fármacos
Antipsicóticos/farmacologia
Antígeno Nuclear de Célula em Proliferação/efeitos dos fármacos
Fator Neurotrófico Derivado do Encéfalo/efeitos dos fármacos
Células Intersticiais do Testículo/efeitos dos fármacos
-Testículo/citologia
Imuno-Histoquímica
Ratos Wistar
Caspase 3/efeitos dos fármacos
Limites: Animais
Masculino
Ratos
Responsável: CL1.1 - Biblioteca Central


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Id: biblio-889091
Autor: de Deus, JL; Dagostin, ALA; Varanda, WA.
Título: Nitric oxide modulates ATP-evoked currents in mouse Leydig cells
Fonte: Braz. j. med. biol. res = Rev. bras. pesqui. méd. biol;51(5):e6693, 2018. graf.
Idioma: en.
Projeto: FAPESP.
Resumo: Testosterone synthesis within Leydig cells is a calcium-dependent process. Intracellular calcium levels are regulated by different processes including ATP-activated P2X purinergic receptors, T-type Ca2+ channels modulated by the luteinizing hormone, and intracellular calcium storages recruited by a calcium-induced calcium release mechanism. On the other hand, nitric oxide (NO) is reported to have an inhibitory role in testosterone production. Based on these observations, we investigated the interaction between the purinergic and nitrergic systems in Leydig cells of adult mice. For this purpose, we recorded ATP-evoked currents in isolated Leydig cells using the whole cell patch clamp technique after treatment with L-NAME (300 μM and 1 mM), L-arginine (10, 100, 300, and 500 μM), ODQ (300 μM), and 8-Br-cGMP (100 μM). Our results show that NO produced by Leydig cells in basal conditions is insufficient to change the ATP-evoked currents and that extra NO provided by adding 300 μM L-arginine positively modulates the current through a mechanism involving the NO/cGMP signaling pathway. Thus, we report an interaction between the nitrergic and purinergic systems in Leydig cells and suggest that Ca2+ entry via the purinergic receptors can be regulated by NO.
Descritores: Trifosfato de Adenosina/fisiologia
Receptores Purinérgicos/metabolismo
Células Intersticiais do Testículo/fisiologia
Óxido Nítrico/fisiologia
-Arginina/administração & dosagem
Arginina/metabolismo
Tionucleotídeos/administração & dosagem
Tionucleotídeos/metabolismo
Potenciais de Ação
Células Cultivadas
GMP Cíclico/administração & dosagem
GMP Cíclico/análogos & derivados
GMP Cíclico/metabolismo
Técnicas de Patch-Clamp
NG-Nitroarginina Metil Éster/administração & dosagem
NG-Nitroarginina Metil Éster/metabolismo
Óxido Nítrico/biossíntese
Limites: Animais
Masculino
Camundongos
Responsável: BR1.1 - BIREME


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Id: lil-652490
Autor: Quevedo Perales, Germán; Gamez Arias, Roberto.
Título: Uso de laparoscopia para el diagnostico y tratamiento de testiculo no palpable / Use of laparoscopy for diagnosis and treatment of nonpalpable testis
Fonte: Rev. Soc. Boliv. Pediatr;48(2):85-88, 2009.
Idioma: es.
Resumo: El objetivo del presente trabajo es el de evaluar los resultados y las ventajas del uso de laparoscopía en el diagnóstico como el tratamiento de los testículos no palpables.
Descritores: Criptorquidismo
Células Intersticiais do Testículo
Testículo
Limites: Humanos
Responsável: BO2.1 - Centro de Información y Documentación


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Id: lil-651819
Autor: Martins, M. R. F. B; Silva, P. J. R; Martins, B. B; Zoca, S. M; Freitas, M. A; Batista, T. C.
Título: Morphological aspects of spermatogenic cells at different stagesof sexual maturation in black isogenic C57BL/6/Uni mice / Aspectos morfológicos de células de la espermatogénesis en diferentes etapas de maduración sexual del ratón negro isogénico C57BL/6/Uni
Fonte: Int. j. morphol;30(2):493-498, jun. 2012. ilus.
Idioma: en.
Resumo: In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and leptotene was observed, whereas in the adluminal compartment Golgi phase spermatids we observed the presence of acrosomal granule. In group II, the cells of the seminiferous epithelium were developed and it was observed in round spermatids cephalic hood phase plus many elongated spermatids in acrosome phase and Sertoli cells. In group III, 60 days old, it was found that seminiferous epithelium which was of the tubules had elongated spermatids in acrosome phase and maturation, with elongated nuclei and acrosomal system typical of spermiation in the presence of sperm and residual bodies near the tubular lumen. Therefore morphological evolution of germ cell testicular spermatids can be checked and recognized in its four phases: golgi, cap, acrosome and maturation over the age of the animal.

Con el fin de estudiar los cambios morfológicos que ocurren en las células de los testículos del ratón negro isogénico C57BL/6/Uni en tres períodos durante el proceso espermatogenético, se utilizaron 15 ratones divididos en 3 grupos de 5 animales con 40, 50 y 60 días de edad. Los ratones fueron sacrificados y pesados. Luego se pesaron y midieron los testículos, para ser finalmete procesados histológicamente y teñidos con HE, PAS y tricrómico Massom-H y evaluados bajo microscopía de luz. Se observó en el grupo I con 40 días de edad que los túbulos seminíferos tenían un lumen pequeño con escaza cantidad de células tubulares intersticiales. En el epitelio seminífero se identificaron espermatogonias tipo A, intermedio y B, que ocuparon el compartimiento adbasal y entremezcladas con estas células, se observó los espermatocitos I en paquiteno y leptoteno, mientras que en el compartimiento adluminal se observaron espermátidas de fase Golgi en presencia de gránulos acrosomales. En el grupo II, las células del epitelio seminífero se desarrollaron y fue posible observar espermátidas redondas en fase de capuz cefálico además de numerosas espermátidas elongadas en fase acrosómica y células sustentaculares. En el grupo III, de 60 días de edad, se encontró que el epitelio seminífero que revestía los túbulos presentaban espermátidas alargadas en fase acrosómica y de maduración, con sus núcleos alargados y el sistema acrosomal típico de la espermiación, con la presencia de espermatozoides y cuerpos residuales cerca del lumen tubular. Así, se puede comprobar con el paso de la edad del animal, la evolución morfológica de las células germinativas testiculares y reconocer las espermátidas en sus cuatro fases: golgi, capuchón, acrosomal y de maduración.
Descritores: Células Intersticiais do Testículo
Maturidade Sexual
Espermatogênese
Testículo/anatomia & histologia
-RATONES CONSANGUINEOS CABDOMENABDOMINAL INJURIESBL
Testículo/citologia
Testículo/ultraestrutura
Limites: Animais
Ratos
Responsável: CL1.1 - Biblioteca Central



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